Moc04g25410 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g25410
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr4: 18455517 .. 18455990 (-)
RNA-Seq ExpressionMoc04g25410
SyntenyMoc04g25410
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGGATAAGGATTGGAATGAGATGGATGAGCAAGCCGTTGCGAACAACAGAATGTCGTTGTCGATGAATGTTTGTAGTCTGGTGATGAAAGAGACTACAACGAAGGAATTGTTGAAGGCCTTGTAAGACAGGTATGAGAAACCTTCTGTCGACACTAAAATACTTCTATGGATGAAGTATTTTGATATCCACATGGAGGAGGGAACCTCGGTGAATTCCCACATTAATGAGCTTACCAATATCTTGAACAAATTAGAAGGGATGGGTGTCAAGATTGAGGAAGAGATGAAGGCTATGATGCTGTTGACATATTTCCCTGACAGTTGGGAGACGATGAAGACCGCGGTGTCGAATTCGCTAGGGGAAAATAGCTTGAAAGTTTTAGCTATTTGTGATGCCGCCTTATTTGAGGAAGCCCAGAGAAAATTAGGAAAAATGTGCATCTACTTCAGGGGTAGAAAACGGGGTTGA

mRNA sequence

ATGACGGATAAGGATTGGAATGAGATGGATGAGCAAGCCGTTGCGAACAACAGAATGTCGTATGAGAAACCTTCTGTCGACACTAAAATACTTCTATGGATGAAGTATTTTGATATCCACATGGAGGAGGGAACCTCGGTGAATTCCCACATTAATGAGCTTACCAATATCTTGAACAAATTAGAAGGGATGGGTGTCAAGATTGAGGAAGAGATGAAGGCTATGATGCTGTTGACATATTTCCCTGACAGTTGGGAGACGATGAAGACCGCGGTGTCGAATTCGCTAGGGGAAAATAGCTTGAAAGTTTTAGCTATTTGTGATGCCGCCTTATTTGAGGAAGCCCAGAGAAAATTAGGAAAAATGTGCATCTACTTCAGGGGTAGAAAACGGGGTTGA

Coding sequence (CDS)

ATGACGGATAAGGATTGGAATGAGATGGATGAGCAAGCCGTTGCGAACAACAGAATGTCGTATGAGAAACCTTCTGTCGACACTAAAATACTTCTATGGATGAAGTATTTTGATATCCACATGGAGGAGGGAACCTCGGTGAATTCCCACATTAATGAGCTTACCAATATCTTGAACAAATTAGAAGGGATGGGTGTCAAGATTGAGGAAGAGATGAAGGCTATGATGCTGTTGACATATTTCCCTGACAGTTGGGAGACGATGAAGACCGCGGTGTCGAATTCGCTAGGGGAAAATAGCTTGAAAGTTTTAGCTATTTGTGATGCCGCCTTATTTGAGGAAGCCCAGAGAAAATTAGGAAAAATGTGCATCTACTTCAGGGGTAGAAAACGGGGTTGA

Protein sequence

MTDKDWNEMDEQAVANNRMSYEKPSVDTKILLWMKYFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNSLGENSLKVLAICDAALFEEAQRKLGKMCIYFRGRKRG
Homology
BLAST of Moc04g25410 vs. NCBI nr
Match: KAG7593230.1 (Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 91.7 bits (226), Expect = 5.2e-15
Identity = 42/97 (43.30%), Postives = 64/97 (65.98%), Query Frame = 0

Query: 21  YEKPSVDTKILLWMKYFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTY 80
           YEKPS + K+ L  K F + MEEG  V +H+NE   I+N+L  + ++ ++E++A++LL  
Sbjct: 91  YEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTIVNQLSSVEIEFDDEVRALILLAS 150

Query: 81  FPDSWETMKTAVSNSLGENSLKVLAICDAALFEEAQR 118
            P+SWE M+ AVSNS+G   LK + + D  L EE +R
Sbjct: 151 LPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

BLAST of Moc04g25410 vs. NCBI nr
Match: KAG7584790.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 91.7 bits (226), Expect = 5.2e-15
Identity = 42/97 (43.30%), Postives = 64/97 (65.98%), Query Frame = 0

Query: 21  YEKPSVDTKILLWMKYFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTY 80
           YEKPS + K+ L  K F + MEEG  V +H+NE   I+N+L  + ++ ++E++A++LL  
Sbjct: 91  YEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTIVNQLSSVEIEFDDEVRALILLAS 150

Query: 81  FPDSWETMKTAVSNSLGENSLKVLAICDAALFEEAQR 118
            P+SWE M+ AVSNS+G   LK + + D  L EE +R
Sbjct: 151 LPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

BLAST of Moc04g25410 vs. NCBI nr
Match: KAG7561662.1 (Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 90.9 bits (224), Expect = 8.9e-15
Identity = 41/97 (42.27%), Postives = 64/97 (65.98%), Query Frame = 0

Query: 21  YEKPSVDTKILLWMKYFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTY 80
           YEKPS + K+ L  K F + MEEG  V +H+NE   I+N+L  + ++ ++E++A++L+  
Sbjct: 91  YEKPSANNKVFLMKKLFHLKMEEGGPVATHVNEFNTIVNQLSSVEIEFDDEVRALILIAS 150

Query: 81  FPDSWETMKTAVSNSLGENSLKVLAICDAALFEEAQR 118
            P+SWE M+ AVSNS+G   LK + + D  L EE +R
Sbjct: 151 LPNSWEPMRAAVSNSVGNQKLKFVDVRDRILGEEVRR 187

BLAST of Moc04g25410 vs. NCBI nr
Match: RZC29599.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Glycine soja])

HSP 1 Score: 90.9 bits (224), Expect = 8.9e-15
Identity = 52/143 (36.36%), Postives = 77/143 (53.85%), Query Frame = 0

Query: 1    MTDKDWNEMDEQAVANNRMS-------------------------YEKPSVDTKILLWMK 60
            M  ++WN +D QA+   R++                         YEKPS   K+ L  +
Sbjct: 917  MKQEEWNLLDRQALGVIRLTLAKNVAFNIVNEKTTAGLMKALSDMYEKPSAANKVYLMRR 976

Query: 61   YFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNS 119
             F++ M EG SV  HINE   IL +LE + +K E+E+KA++LL+  PDSW    TAVS+S
Sbjct: 977  LFNLKMGEGISVTDHINEFNTILAQLESVQIKFEDEVKALILLSSLPDSWAATVTAVSSS 1036

BLAST of Moc04g25410 vs. NCBI nr
Match: KAD5507982.1 (hypothetical protein E3N88_15685 [Mikania micrantha])

HSP 1 Score: 90.5 bits (223), Expect = 1.2e-14
Identity = 48/114 (42.11%), Postives = 70/114 (61.40%), Query Frame = 0

Query: 21  YEKPSVDTKILLWMKYFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTY 80
           YEKPS   K+ L  + F + M EG+SV  HIN L +IL++L  +G+K ++E KA++LL+ 
Sbjct: 92  YEKPSAANKVFLMRELFTMRMREGSSVTEHINNLNSILSRLSSVGMKFDDETKAVLLLSS 151

Query: 81  FPDSWETMKTAVSNSLGENSLKVLAICDAALFEEAQR---KLGKMCIYFRGRKR 132
            PDSW    TAV+NS+G + L    I D  L E+ +R   K G   +++ GR R
Sbjct: 152 LPDSWSGTVTAVTNSVGTDGLTFEKIRDLVLGEDVRRRGAKAGSSEMFYVGRGR 205

BLAST of Moc04g25410 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 4.0e-10
Identity = 41/143 (28.67%), Postives = 71/143 (49.65%), Query Frame = 0

Query: 1   MTDKDWNEMDEQA-----------VANNRMS--------------YEKPSVDTKILLWMK 60
           M  +DW ++DE+A           V NN +               Y   ++  K+ L  +
Sbjct: 47  MKAEDWADLDERAASAIRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQ 106

Query: 61  YFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNS 119
            + +HM EGT+  SH+N    ++ +L  +GVKIEEE KA++LL   P S++ + T + + 
Sbjct: 107 LYALHMSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILH- 166

BLAST of Moc04g25410 vs. ExPASy TrEMBL
Match: A0A0D3AEM1 (CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.7e-16
Identity = 44/97 (45.36%), Postives = 65/97 (67.01%), Query Frame = 0

Query: 21  YEKPSVDTKILLWMKYFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTY 80
           YEKPS ++K+ L  K F + MEEG  V +HINE   I+N+L  + ++ E+E++A++LL  
Sbjct: 91  YEKPSANSKVFLMKKLFHLKMEEGGLVTAHINEFNTIVNQLSSVEIEFEDEVRALILLAS 150

Query: 81  FPDSWETMKTAVSNSLGENSLKVLAICDAALFEEAQR 118
            P+SWE+M+ AVSNS+G   LK   + D  L EE +R
Sbjct: 151 LPNSWESMRVAVSNSVGTQKLKFNDVRDRILAEEVRR 187

BLAST of Moc04g25410 vs. ExPASy TrEMBL
Match: A0A2N9GTI4 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30874 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-15
Identity = 47/124 (37.90%), Postives = 72/124 (58.06%), Query Frame = 0

Query: 1   MTDKDWNEMDEQAVANNRM------SYEKPSVDTKILLWMKYFDIHMEEGTSVNSHINEL 60
           M D +W  +D Q      +       YEKPS + K+ L  K F++ M EGT+V  H+NE 
Sbjct: 47  MEDAEWTLLDRQEKTTAELMTALCGMYEKPSANNKVHLMKKLFNLKMAEGTAVAQHLNEF 106

Query: 61  TNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNSLGENSLKVLAICDAALFEE 119
             I N+L  + ++ ++E++A+++L   P+SWE M+ AVSNS G+  LK   I D  L EE
Sbjct: 107 NTITNQLSSVEIEFDDEIRALIVLASLPNSWEAMRMAVSNSAGKGKLKYNDIRDLILGEE 166

BLAST of Moc04g25410 vs. ExPASy TrEMBL
Match: A0A2N9EUG1 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6315 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-15
Identity = 47/124 (37.90%), Postives = 72/124 (58.06%), Query Frame = 0

Query: 1   MTDKDWNEMDEQAVANNRM------SYEKPSVDTKILLWMKYFDIHMEEGTSVNSHINEL 60
           M D +W  +D Q      +       YEKPS + K+ L  K F++ M EGT+V  H+NE 
Sbjct: 47  MEDAEWTLLDRQEKTTAELMTALCGMYEKPSANNKVHLMKKLFNLKMAEGTAVAQHLNEF 106

Query: 61  TNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNSLGENSLKVLAICDAALFEE 119
             I N+L  + ++ ++E++A+++L   P+SWE M+ AVSNS G+  LK   I D  L EE
Sbjct: 107 NTITNQLSSVEIEFDDEIRALIVLASLPNSWEAMRMAVSNSAGKGKLKYNDIRDLILGEE 166

BLAST of Moc04g25410 vs. ExPASy TrEMBL
Match: A0A2N9HHD8 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS39035 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-15
Identity = 47/124 (37.90%), Postives = 72/124 (58.06%), Query Frame = 0

Query: 1   MTDKDWNEMDEQAVANNRM------SYEKPSVDTKILLWMKYFDIHMEEGTSVNSHINEL 60
           M D +W  +D Q      +       YEKPS + K+ L  K F++ M EGT+V  H+NE 
Sbjct: 47  MEDAEWTLLDRQEKTTAELMTALCGMYEKPSANNKVHLMKKLFNLKMAEGTAVAQHLNEF 106

Query: 61  TNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNSLGENSLKVLAICDAALFEE 119
             I N+L  + ++ ++E++A+++L   P+SWE M+ AVSNS G+  LK   I D  L EE
Sbjct: 107 NTITNQLSSVEIEFDDEIRALIVLASLPNSWEAMRMAVSNSAGKGKLKYNDIRDLILGEE 166

BLAST of Moc04g25410 vs. ExPASy TrEMBL
Match: A0A445M280 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Glycine soja OX=3848 GN=D0Y65_001253 PE=4 SV=1)

HSP 1 Score: 90.9 bits (224), Expect = 4.3e-15
Identity = 52/143 (36.36%), Postives = 77/143 (53.85%), Query Frame = 0

Query: 1    MTDKDWNEMDEQAVANNRMS-------------------------YEKPSVDTKILLWMK 60
            M  ++WN +D QA+   R++                         YEKPS   K+ L  +
Sbjct: 917  MKQEEWNLLDRQALGVIRLTLAKNVAFNIVNEKTTAGLMKALSDMYEKPSAANKVYLMRR 976

Query: 61   YFDIHMEEGTSVNSHINELTNILNKLEGMGVKIEEEMKAMMLLTYFPDSWETMKTAVSNS 119
             F++ M EG SV  HINE   IL +LE + +K E+E+KA++LL+  PDSW    TAVS+S
Sbjct: 977  LFNLKMGEGISVTDHINEFNTILAQLESVQIKFEDEVKALILLSSLPDSWAATVTAVSSS 1036

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7593230.15.2e-1543.30Pentatricopeptide repeat [Arabidopsis thaliana x Arabidopsis arenosa][more]
KAG7584790.15.2e-1543.30Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa][more]
KAG7561662.18.9e-1542.27Zinc finger CCHC-type superfamily [Arabidopsis thaliana x Arabidopsis arenosa][more]
RZC29599.18.9e-1536.36Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Glycine soja][more]
KAD5507982.11.2e-1442.11hypothetical protein E3N88_15685 [Mikania micrantha][more]
Match NameE-valueIdentityDescription
P109784.0e-1028.67Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
A0A0D3AEM18.7e-1645.36CCHC-type domain-containing protein OS=Brassica oleracea var. oleracea OX=109376... [more]
A0A2N9GTI41.9e-1537.90Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS30874 PE=4 SV=1[more]
A0A2N9EUG11.9e-1537.90Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6315 PE=4 SV=1[more]
A0A2N9HHD81.9e-1537.90Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS39035 PE=4 SV=1[more]
A0A445M2804.3e-1536.36Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Glycine soja OX=3... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 51..71
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 20..106
e-value: 4.7E-21
score: 75.0
NoneNo IPR availablePANTHERPTHR34676:SF1ZINC FINGER, CCHC-TYPE, TUBBY C-TERMINAL-LIKE DOMAIN PROTEIN-RELATEDcoord: 21..118
NoneNo IPR availablePANTHERPTHR34676FAMILY NOT NAMEDcoord: 21..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g25410.1Moc04g25410.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0044260 cellular macromolecule metabolic process
biological_process GO:0006807 nitrogen compound metabolic process
biological_process GO:0044238 primary metabolic process
molecular_function GO:1901363 heterocyclic compound binding
molecular_function GO:0097159 organic cyclic compound binding
molecular_function GO:0016772 transferase activity, transferring phosphorus-containing groups