Moc04g25290 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc04g25290 vs. NCBI nr
Match: XP_022147189.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111016200 [Momordica charantia])

HSP 1 Score: 2336.6 bits (6054), Expect = 0.0e+00
Identity = 1292/1940 (66.60%), Postives = 1317/1940 (67.89%), Query Frame = 0

Query: 71   MPQYTTYNPLYDVPVGQYPHPFVKGAQQIPTNIIFREPEQMMSPPTVLYLGGLPAKTDLV 130
            MPQYTTYNPLYDVPVGQY HPFVKGAQQIPTNIIFREPEQMMSPPTVL LG L AKTD V
Sbjct: 1    MPQYTTYNPLYDVPVGQYLHPFVKGAQQIPTNIIFREPEQMMSPPTVLNLGDLLAKTDPV 60

Query: 131  GQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGLVILPKF-------------- 190
            GQNAPSNEKFEVL+ERLRAIE TDVFGNIDASQLC VSGLVI PK               
Sbjct: 61   GQNAPSNEKFEVLKERLRAIERTDVFGNIDASQLCSVSGLVIPPKLKVPEFEKYNGSSCP 120

Query: 191  ---------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQLDSSHVGSWKNQADSFLKQYK 250
                     KMAAYVQNDKLLIHCFQDSLS PASRWYMQLDSSHVGSWKN ADSFLKQYK
Sbjct: 121  KNHLXMYCRKMAAYVQNDKLLIHCFQDSLSGPASRWYMQLDSSHVGSWKNLADSFLKQYK 180

Query: 251  HNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPPLTDKELSAMFINTLKHPFYD 310
            HNIDMAPDRLDLQRMEKKST+SFKEYAQRWRDT AQ+QPPL DKELSAMFINTLKHPFYD
Sbjct: 181  HNIDMAPDRLDLQRMEKKSTKSFKEYAQRWRDTAAQVQPPLIDKELSAMFINTLKHPFYD 240

Query: 311  RMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAKKASHSKKKEGEVQMVGADRH 370
            RMIGSASTNFSDIMTIGERIEYGVRHGRITST DEPLAAKKASHSKKKEGEVQMVGADRH
Sbjct: 241  RMIGSASTNFSDIMTIGERIEYGVRHGRITSTTDEPLAAKKASHSKKKEGEVQMVGADRH 300

Query: 371  SWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYASQNFRPPA---------SQN 430
            SWKQQPY RTP+Y+PYYYPTPYGYNQPFVNNATSHY PYASQNFRPPA         SQN
Sbjct: 301  SWKQQPYRRTPQYSPYYYPTPYGYNQPFVNNATSHYYPYASQNFRPPASQNFQLTPTSQN 360

Query: 431  FQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY 490
            FQPRGQQHNTFYTQGQQ NRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY
Sbjct: 361  FQPRGQQHNTFYTQGQQNNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY 420

Query: 491  PRW---------------------------VQALIKVGWLNFKKENGPDVSKNPLPNHQN 550
            PRW                           VQALIK GW NFKKENG DVSK  L NHQN
Sbjct: 421  PRWYDANARCDYHAGAIXHSTENCTXLKYRVQALIKAGWXNFKKENGXDVSKXXLXNHQN 480

Query: 551  VQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFH 610
            VQINAIECQ IESKSKVA+ITTPM ELFEILLGSGY+SVEYLCP  KYKGYDESLTC FH
Sbjct: 481  VQINAIECQGIESKSKVABITTPMXELFEILLGSGYISVEYLCP--KYKGYDESLTCXFH 540

Query: 611  AGAKG--------------------------------INIVEDILFVEGSSDSLKPKLLT 670
             GAKG                                 N+VEDIL  EGSSDSLKPK LT
Sbjct: 541  XGAKGHSLEQCNXFRMKVQELLDSKILTXANSHXKKXTNVVEDILVAEGSSDSLKPKPLT 600

Query: 671  VFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECKVTVGQDISFPSLPVDNITGV 730
            +FYREKPDA SCSRKP  I +P PFEYKSSKAVPWKYECKVTVGQD+S PSLPVDNITGV
Sbjct: 601  IFYREKPDAPSCSRKPXXITVPXPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGV 660

Query: 731  G----------------------------------------------------------- 790
            G                                                           
Sbjct: 661  GGLTRTGRCYTPDSLLKRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDARDELVEA 720

Query: 791  --------------EETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAHQNTLLE 850
                          EETQEFLKLVKQSEYKVIEQLGRTPA ISILSLLLSSEAHQN LLE
Sbjct: 721  IVVKDVSPKQPMSEEETQEFLKLVKQSEYKVIEQLGRTPANISILSLLLSSEAHQNALLE 780

Query: 851  ALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHISVKCKNFLIAK 910
            ALKQAFVSQDITVDNLSNVVGNIT SSSI+FTDEEIPPEGTGHTKALHISVKCKNFLIAK
Sbjct: 781  ALKQAFVSQDITVDNLSNVVGNITASSSISFTDEEIPPEGTGHTKALHISVKCKNFLIAK 840

Query: 911  VLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEIPIQIGPCTFD 970
            VLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAV GDIEIPIQIGPCTFD
Sbjct: 841  VLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQIGPCTFD 900

Query: 971  ITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILVSRLASM 1030
            ITFQVMDITS YSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILVSR ASM
Sbjct: 901  ITFQVMDITSAYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILVSRFASM 960

Query: 1031 PYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDKLLRMAKNTKK 1090
             YVE AEEAFESSFQSFEIANATTLHGKFGRPKP+LLETAFKGDNGSLDKLLRMAKNTKK
Sbjct: 961  SYVEVAEEAFESSFQSFEIANATTLHGKFGRPKPRLLETAFKGDNGSLDKLLRMAKNTKK 1020

Query: 1091 FGLGYKPSRGDIIRVRSLEKAKRLLRFENEELDYPRRTVPPLSHSFRKVGTIHQEYDESS 1150
            FGLGYKPSRGDIIRVRSLEKAKRL RFENEE DYPRR VPPL+HSFR  GTIHQEYDESS
Sbjct: 1021 FGLGYKPSRGDIIRVRSLEKAKRLSRFENEERDYPRRIVPPLTHSFRSAGTIHQEYDESS 1080

Query: 1151 VVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDNDSKYELDTPI 1210
            VVAAVT+EREQV PFVY CPDGFELSNWSVI+LPSFVNNKSNNTEIECDNDSKYELDTPI
Sbjct: 1081 VVAAVTEEREQVGPFVYLCPDGFELSNWSVIKLPSFVNNKSNNTEIECDNDSKYELDTPI 1140

Query: 1211 YNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETINLGSQAEAKEIKIGTHMSSESR 1270
            Y IESDEEIDDEPSAELLRMLEEEEKMLGPHEELTET+NLGSQAEAKEIKIGTHMSSESR
Sbjct: 1141 YIIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETLNLGSQAEAKEIKIGTHMSSESR 1200

Query: 1271 KKLIELLHD------------LGCSTSKTIH----------------------------- 1330
            KKLIELLH+            LG  T   +H                             
Sbjct: 1201 KKLIELLHEYADVFAWSYXDMLGLDTDIVVHKLPINPEFKPMRQKLRKMRPDMLIKIKNE 1260

Query: 1331 ------------------------------------------------------------ 1390
                                                                        
Sbjct: 1261 VRKQIDAGFLTVSNYPEWVANIVPVPKKNGQVRMCVDYRDLNRASPKDNFPLPHIDVLVD 1320

Query: 1391 -----------------------------------------------------------V 1450
                                                                       V
Sbjct: 1321 NTAGFSTFSFMDGFSGYNQIKMAPEDREKTTFITLWGTFCYKVMSFGLKNAGATYQRAMV 1380

Query: 1451 VLYH--------------MAHFKDG----------------------------------- 1465
             L+H              +A  K G                                   
Sbjct: 1381 TLFHDLMHKEIEVYVDDMIAKSKQGEEHTTILRKLFDRLRKFKLKLNSNKCIFGATTGKL 1440

BLAST of Moc04g25290 vs. NCBI nr
Match: XP_022143495.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111013372 [Momordica charantia])

HSP 1 Score: 1979.1 bits (5126), Expect = 0.0e+00
Identity = 1139/1886 (60.39%), Postives = 1163/1886 (61.66%), Query Frame = 0

Query: 121  GGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGLVILPKF---- 180
            GGLPAKTD VGQNAPSNEKFEVL+ERLRAIEGTDVFGNIDASQLCLVS LVI PKF    
Sbjct: 62   GGLPAKTDPVGQNAPSNEKFEVLKERLRAIEGTDVFGNIDASQLCLVSRLVIPPKFKVPE 121

Query: 181  -------------------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQLDSSHVGSWKN 240
                               KMAAYVQNDKLLIHCFQDSLS PASRWYMQLDSSHVGSWKN
Sbjct: 122  FEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSSPASRWYMQLDSSHVGSWKN 181

Query: 241  QADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPPLTDKELSAMF 300
             ADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDT AQ+QPPLTDKELS MF
Sbjct: 182  LADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTAAQVQPPLTDKELSXMF 241

Query: 301  INTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAKKASHSKKKEG 360
            INTLKHPFYDRM+GSASTNFSDIM IGERIEYGVRHGRITSTADEPLAAKK SHSKKKEG
Sbjct: 242  INTLKHPFYDRMVGSASTNFSDIMAIGERIEYGVRHGRITSTADEPLAAKKTSHSKKKEG 301

Query: 361  EVQMVGADRHSWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYASQNFRPPASQ 420
            E                                                           
Sbjct: 302  E----------------------------------------------------------- 361

Query: 421  NFQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPP 480
                                                            LA VPVDPIQPP
Sbjct: 362  ------------------------------------------------LAHVPVDPIQPP 421

Query: 481  YPRW---------------------------VQALIKVGWLNFKKENGPDVSKNPLPNHQ 540
            YPRW                           VQALIK GWLNFKKENGP+VS NPLPNH 
Sbjct: 422  YPRWCDANARCDYHTGAIGHSIENCTALKYRVQALIKAGWLNFKKENGPBVSNNPLPNHX 481

Query: 541  NVQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPF 600
            NVQINAIECQ IESKSKVA+ITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPF
Sbjct: 482  NVQINAIECQEIESKSKVADITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPF 541

Query: 601  HAGA--------------------------------KGINIVEDI-----LFVEGSSDSL 660
            HAGA                                KGIN+VED+        EGSSD+L
Sbjct: 542  HAGAKGHALEQCNSFRMIVQELLDSKILTVANSHQKKGINVVEDVSVAEGSIAEGSSDAL 601

Query: 661  KPKLLTVFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECKVTVGQDISFPSLPV 720
            KPK LT+FY EKPDA +CSRKPITI +PAPFEYKSSKAVPWKYECKVTVGQD+S P LPV
Sbjct: 602  KPKRLTIFYSEKPDAPNCSRKPITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPPLPV 661

Query: 721  DNITGVG----------------------------------------------------- 780
            DNITGVG                                                     
Sbjct: 662  DNITGVGGLTXTGRCYTPDSLLKRVSETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVH 721

Query: 781  --------------------EETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAH 840
                                EE QEFLKLVKQSEYKV EQLGRTPAKISILSLLLSSEAH
Sbjct: 722  DELVEAIVVKDVSPKQHVFEEEIQEFLKLVKQSEYKVTEQLGRTPAKISILSLLLSSEAH 781

Query: 841  QNTLLEALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHISVKCK 900
            +NTLLE LKQAFVSQDITVDNLSNVVGNIT SSSITFTDEEIPPEGTGHTKALHISVKCK
Sbjct: 782  RNTLLEXLKQAFVSQDITVDNLSNVVGNITASSSITFTDEEIPPEGTGHTKALHISVKCK 841

Query: 901  NFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEIPIQI 960
            NFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAV GDIEIPIQI
Sbjct: 842  NFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQI 901

Query: 961  GPCTFDITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILV 1020
            GPCTFDITFQVMDITSTYSFLLGR WIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILV
Sbjct: 902  GPCTFDITFQVMDITSTYSFLLGRLWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILV 961

Query: 1021 SRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDKLLRM 1080
            SRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKP+LLETAFKGDN SLDKLLRM
Sbjct: 962  SRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPRLLETAFKGDNESLDKLLRM 1021

Query: 1081 AKNTKKFGLGYKPSRGDIIRVRSLEKAKRLLRFENEELDYPRRTVPPLSHSFRKVGTIHQ 1140
            AKNTKKFGLGYKPSRGDIIRVRSLEKAKRL RFENEE DYPRRTVPPLSHSFR  GTIHQ
Sbjct: 1022 AKNTKKFGLGYKPSRGDIIRVRSLEKAKRLSRFENEERDYPRRTVPPLSHSFRSAGTIHQ 1081

Query: 1141 EYDESSVVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDNDSKY 1200
            EYD SSVVAAVT+EREQV PFVYPCPDGFELSNWSV            NTEIECDNDSKY
Sbjct: 1082 EYDGSSVVAAVTEEREQVRPFVYPCPDGFELSNWSV------------NTEIECDNDSKY 1141

Query: 1201 ELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETINLGSQAEAKEIKIGTH 1260
            ELDTPIYNIESD+EIDDEPSAELLRMLEEEEKMLGPHEELTET+NLGSQAEAKEIKIGTH
Sbjct: 1142 ELDTPIYNIESDKEIDDEPSAELLRMLEEEEKMLGPHEELTETLNLGSQAEAKEIKIGTH 1201

Query: 1261 MSSESRKKLIELLHDL------------GCSTSKTIH----------------------- 1320
            MSSESRKKLIELLH+             G  T   +H                       
Sbjct: 1202 MSSESRKKLIELLHEYADVFAWSYQDMPGLDTDIVVHKLQINPKFKPVRQKLRKMRPDML 1261

Query: 1321 ------------------------------------------------------------ 1380
                                                                        
Sbjct: 1262 IKIKDEVRKQIDAGFLTISNYPEWVANIVPVPKKNGQVRMCVDYRDLNRASPKDNFPLPH 1321

Query: 1381 ------------------------------------------------------------ 1440
                                                                        
Sbjct: 1322 IDVLVDNTAGFSTFSFMDGFSGYNQIKMAPEDREKTTFITLWGTFYYKVMXFGLKNAGAT 1381

Query: 1441 -----VVLYH--------------MAHFKDG----------------------------- 1465
                 V L+H              +A  K G                             
Sbjct: 1382 YQRAMVTLFHDLMHKEIEVYVDDMIAKSKQGEKHTTILRKLFDRLRKFKLKLNPNKCIFG 1441

BLAST of Moc04g25290 vs. NCBI nr
Match: XP_022158986.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111025431 [Momordica charantia])

HSP 1 Score: 1908.3 bits (4942), Expect = 0.0e+00
Identity = 1056/1561 (67.65%), Postives = 1125/1561 (72.07%), Query Frame = 0

Query: 111  MMSPPTVLYLGGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGL 170
            MMSPPTVL LGGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGT VFGNIDASQLCLVSGL
Sbjct: 1    MMSPPTVLNLGGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTYVFGNIDASQLCLVSGL 60

Query: 171  VILPKF-----------------------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQL 230
            VI PKF                       KMAAYVQNDKLLIHCFQDSLS PASRWYMQL
Sbjct: 61   VIPPKFKVPEFEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSGPASRWYMQL 120

Query: 231  DSSHVGSWKNQADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPP 290
            DSS+VGSWKN ADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDT AQ+QPP
Sbjct: 121  DSSNVGSWKNLADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTAAQVQPP 180

Query: 291  LTDKELSAMFINTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAK 350
            LTDKELSAMFINTLKHPFYDRMIG+ASTNFSDIMTIGERIEYGVRHGRITST DEPLAAK
Sbjct: 181  LTDKELSAMFINTLKHPFYDRMIGNASTNFSDIMTIGERIEYGVRHGRITSTVDEPLAAK 240

Query: 351  KASHSKKKEGEVQMVGADRHSWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYA 410
            KASHSKKKEGEVQMVGADRHSWKQQPY RTPRYTPYYYPTPYGYNQPFVNNATSHYSPY 
Sbjct: 241  KASHSKKKEGEVQMVGADRHSWKQQPYSRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYT 300

Query: 411  SQNFRP---------PASQNFQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLP 470
             QNFRP         PASQNFQPRGQQHNT YTQ QQTNRGARKQTQFDPIPMTYTELLP
Sbjct: 301  FQNFRPPASQNFQPTPASQNFQPRGQQHNTLYTQEQQTNRGARKQTQFDPIPMTYTELLP 360

Query: 471  QLFQNNQLAPVPVDPIQPPYPRW---------------------------VQALIKVGWL 530
            QLFQNNQLAPVPVDPIQPPYPRW                           VQALIK GWL
Sbjct: 361  QLFQNNQLAPVPVDPIQPPYPRWYDTNARCDYHAGAIGHSTENCTALKYRVQALIKAGWL 420

Query: 531  NFKKENGPDVSKNPLPNHQNVQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVE 590
            NFKKENGPDVSKNPLPNHQNVQINAIECQ IESKSKVA+I TPM ELFEILLGSGYVSVE
Sbjct: 421  NFKKENGPDVSKNPLPNHQNVQINAIECQEIESKSKVADIRTPMVELFEILLGSGYVSVE 480

Query: 591  YLCPNLKYKGYDESLTCPFHAGA--------------------------------KGINI 650
            YLCPNLKYKGYDESLTCPFHAGA                                KGINI
Sbjct: 481  YLCPNLKYKGYDESLTCPFHAGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINI 540

Query: 651  VEDILFVEGSSDSLKPKLLTVFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECK 710
            VED+   EGSSD+LKPK LT+FY EKP+A +CSRKPITI +PAPFEYKSSKAVPWKY+CK
Sbjct: 541  VEDVSVAEGSSDALKPKCLTIFYSEKPNAPNCSRKPITITVPAPFEYKSSKAVPWKYQCK 600

Query: 711  VTVGQDISFPSLPVDNITGVG--------------------------------------- 770
            VTVGQD+S P LP+DNITGVG                                       
Sbjct: 601  VTVGQDVSSPPLPIDNITGVGGLTRTGRCYTPDSLLKCVNETTSEKNKEKASEKKKEKVE 660

Query: 771  ----------------------------------EETQEFLKLVKQSEYKVIEQLGRTPA 830
                                              EETQE LKLVKQSEYKVIEQLGRTPA
Sbjct: 661  EDKKGKAKLHEDVHDELVEAIVVKDVSPKQPMSEEETQEILKLVKQSEYKVIEQLGRTPA 720

Query: 831  KISILSLLLSSEAHQNTLLEALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEG 890
            KISILSLLLSSEAH+N LLEALKQAFVSQDITVDNLSNVVGNI+ +SSITFTDEEIPPEG
Sbjct: 721  KISILSLLLSSEAHRNALLEALKQAFVSQDITVDNLSNVVGNISXASSITFTDEEIPPEG 780

Query: 891  TGHTKALHISVKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGA 950
            TGHTKALHIS+KCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGA
Sbjct: 781  TGHTKALHISIKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGA 840

Query: 951  RSAVFGDIEIPIQIGPCTFDITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVD 1010
            RSAV GDIEIPIQIGPCTFDITFQVMDITS YSFLLGRPWIHSAGAVPSTLHQKIKFAVD
Sbjct: 841  RSAVVGDIEIPIQIGPCTFDITFQVMDITSAYSFLLGRPWIHSAGAVPSTLHQKIKFAVD 900

Query: 1011 QKLVIISGQEDILVSRLASMPYVEAAEE--------AFESSFQSF-------EIANATTL 1070
            Q  V              S+P+++   +        +F   F  +       E    TTL
Sbjct: 901  QN-VDYRDLNRASPKDNFSLPHIDVLVDNTTGFSTFSFMDGFSGYNQIKMAPEDREKTTL 960

Query: 1071 HGKFGRPKPQLLETAFKGDNGSLDKLL------RMAKNTKKF--GLGYKPSRGDIIRVRS 1130
               +G    +++    K    +  + +       M K  + +   +  K  +G+      
Sbjct: 961  ITLWGTFCYKVMPFGLKNAGATYQRAMVTLFHDLMHKEIEVYVDDMIAKSKQGEEHTTIL 1020

Query: 1131 LEKAKRLLRFE---NEELDYPRRTVPPLSHSFRKVGTIHQEYDESSVVAAVTKEREQVEP 1190
             +   RL +F+   N        T   L         I  + D+   +  +   + Q E 
Sbjct: 1021 RKLFDRLRKFKLKLNPNKCIFGATTGKLLGFVVSQEDIKVDLDKVKAILEMPPPQTQKEV 1080

Query: 1191 FVYPCPDGF---ELSNWSVIELPSFVNNKSNNTEI---EC----DNDSKYELDTPIYNIE 1250
              +     +    +S+ +    P F   + NN  +   +C    D   +Y  D PI    
Sbjct: 1081 REFLGRLNYIARLISHLTATCEPIFKLLRKNNDGVWSEDCQAAFDKIKQYLQDPPIL--- 1140

Query: 1251 SDEEIDDEPSAELLRMLEEEEK----MLGPHEELTETINLGSQAEAKEIKIGTHMSSESR 1310
                +   P   L+  L   E     +LG H++       G + +A           E+R
Sbjct: 1141 ----VPPTPGRPLILYLTVTENSMGCVLGQHDD------SGRKEQAIYYLSKKFTDCETR 1200

Query: 1311 KKLIE-LLHDLGCSTSKTIHVVLYHMAHF--KDGPIKYIFEKPSLSGRIARWQVLLSEYD 1370
               +E     L  +T +    +LY+      K  PIKYIFEKPSLSGRIARWQVLLSEYD
Sbjct: 1201 YSQVEKTCCALAWATRRLRQYMLYYTTWLISKMDPIKYIFEKPSLSGRIARWQVLLSEYD 1260

Query: 1371 IVYVTQKAIKGSALVDYLAQQPINDYIPVKFDFPDEYISTITASEEILDPQTWTMMFDGA 1430
            IVYVT+KAIKGSAL DYLAQQPINDYIPVKFDFPDEYISTITASEE LDPQTWTMMFDGA
Sbjct: 1261 IVYVTRKAIKGSALADYLAQQPINDYIPVKFDFPDEYISTITASEESLDPQTWTMMFDGA 1320

Query: 1431 SNELGHGIGAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVDMKVKKLKVFGD 1465
            SNELGHGIG ILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQ AVDMKVKKLKVFGD
Sbjct: 1321 SNELGHGIGGILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQAAVDMKVKKLKVFGD 1380

BLAST of Moc04g25290 vs. NCBI nr
Match: XP_022157796.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111024415 [Momordica charantia])

HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 872/1572 (55.47%), Postives = 970/1572 (61.70%), Query Frame = 0

Query: 2    MEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAPDPATSSNPVHEPQETPPYPPGYGP 61
            MEDQKAEQEKTRKDIEELREKLD I L LEKGKA  DPATSSNP+HEPQETPPYPPGYGP
Sbjct: 1    MEDQKAEQEKTRKDIEELREKLDXIXLXLEKGKATADPATSSNPIHEPQETPPYPPGYGP 60

Query: 62   PLRPVAEGFMPQYTTYNPLYDVPVGQYPHPFVKGAQQIPTNIIFREPEQMMSPPTVLYLG 121
            PLRPVAEGFMPQYTTYNPLYDVP+GQYPH FVKGAQQIPTNIIFREPEQMMSPPTV  LG
Sbjct: 61   PLRPVAEGFMPQYTTYNPLYDVPIGQYPHTFVKGAQQIPTNIIFREPEQMMSPPTVXNLG 120

Query: 122  GLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGLVILPKF----- 181
            G PAKTD V QNA S EK EVLEERLRAIEGTDVFGNIDASQLCLVSGLVI PKF     
Sbjct: 121  GXPAKTDPVXQNAXSXEKXEVLEERLRAIEGTDVFGNIDASQLCLVSGLVIPPKFKVPEF 180

Query: 182  ------------------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQLDSSHVGSWKNQ 241
                              KM AYVQN KLLIHCFQDSL   ASRWYMQLDSSHVGSWKN 
Sbjct: 181  EKYDGSSCPKNHLIMYCRKMXAYVQNXKLLIHCFQDSLXGXASRWYMQLDSSHVGSWKNL 240

Query: 242  ADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPPLTDKELSAMFI 301
            ADSFLKQYKHNIDMAPDRLDLQRMEK STESFKEYAQRWRDT AQ+QPPLTDKELSAMFI
Sbjct: 241  ADSFLKQYKHNIDMAPDRLDLQRMEKNSTESFKEYAQRWRDTAAQVQPPLTDKELSAMFI 300

Query: 302  NTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAKKASHSKKKEGE 361
            NTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRH RITSTADEPLAAKKASHSKKKEGE
Sbjct: 301  NTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHKRITSTADEPLAAKKASHSKKKEGE 360

Query: 362  VQMVGADRHSWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYASQNFRPPASQN 421
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 422  FQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY 481
                                                           L PVPVDPIQP Y
Sbjct: 421  -----------------------------------------------LTPVPVDPIQPLY 480

Query: 482  PRW---------------------------VQALIKVGWLNFKKENGPDVSKNPLPNHQN 541
            PRW                           VQAL+K GWLNFKKEN PDVSKNPL NHQN
Sbjct: 481  PRWYDANARCDYHAGAIGHSTENCTALKYRVQALLKAGWLNFKKENEPDVSKNPLSNHQN 540

Query: 542  VQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFH 601
            VQINAIECQ IESKSKVA+I TP EELFEILLGSGYVSVEYLCPNLKYK YDESLTCPFH
Sbjct: 541  VQINAIECQGIESKSKVADIRTPKEELFEILLGSGYVSVEYLCPNLKYKEYDESLTCPFH 600

Query: 602  AGA--------------------------------KGINIVEDILFVEGSSDSLKPKLLT 661
            AGA                                KGIN+VED+   EGSSD+LKPK LT
Sbjct: 601  AGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINVVEDVSVAEGSSDALKPKRLT 660

Query: 662  VFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECKVTVGQDISFPSLPVDNITGV 721
            +FY EKPDA SCS+KPITI +PAPFEYKSSKAVPWKY+CKVTVGQD+S P LPVDNIT V
Sbjct: 661  IFYSEKPDAPSCSQKPITITVPAPFEYKSSKAVPWKYQCKVTVGQDVSSPPLPVDNITEV 720

Query: 722  GEETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAHQNTLLEALKQ-----AFVS 781
            G+       L  Q+E K I+           +   +SSE+ +  L+E L +     A+  
Sbjct: 721  GD-------LGSQAEAKEIK-----------IGTHMSSESRKK-LIELLHEYADVFAWSY 780

Query: 782  QDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVDNGSS 841
            QD+   +   VV        ++   E  P           + +K K+  + K +     +
Sbjct: 781  QDMPGLDTDIVV------HKLSINPEFKPVRQKLWKMRPDMLIKIKD-EVRKQIDAGFLT 840

Query: 842  LNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEIPIQIGPCTFDITFQVMDI 901
            ++  P      +PV   + +   + V   D  R++   +  +P        D+       
Sbjct: 841  VSNYPEWVANIVPVPKKNGQ-VRMCVDYRDLNRASPKDNFPLP------HIDVLVDNTAG 900

Query: 902  TSTYSFLLGRPWIHSAGAVPSTLHQKIKF----AVDQKLVIISGQEDILVSRLASMPYVE 961
             ST+SF+ G    +     P    +K  F          V+  G +++  +   +M  V 
Sbjct: 901  FSTFSFMGGFSGXNXIKMAPED-REKTTFITLWGTFCYKVMPFGLKNVGATYQRAM--VT 960

Query: 962  AAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDKL----LRMAKNTKK 1021
               +      + +   +      K G     +L   F       D+L    L++  N   
Sbjct: 961  LFHDLMHKEIEVY--VDDMIAKSKQGEEHTTILRKLF-------DRLRKFKLKLNPNKCI 1020

Query: 1022 FGLGYKPSRGDIIRVRSL----EKAKRLLRFENEELDYPRRTVPPLSHSFRKVGTIHQEY 1081
            FG       G ++    +    +K K +L             +PP        G + +  
Sbjct: 1021 FGATTGKLLGFVVSQEGIKVDPDKVKAILE------------MPPPQTQKEVRGFLGRLN 1080

Query: 1082 DESSVVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDNDSKYEL 1141
              +  ++ +T   E +   +    DG     W            S + +   +   +Y  
Sbjct: 1081 YIARFISHLTATCEPIFKLLRKNNDGV----W------------SEDCQAAFNKIKQYLQ 1140

Query: 1142 DTPIYNIESDEEIDDEPSAELLRMLEEEEK----MLGPHEELTETINLGSQAEAKEIKIG 1201
            D PI        +   P   L+  L   E     +LG H++       G + +A      
Sbjct: 1141 DPPIL-------VPPTPGRPLILYLTVTENSMGCVLGQHDD------SGRKEQAIYYLSK 1200

Query: 1202 THMSSESRKKLIELLHDLGCSTSKTIHVVLYHMAHF------KDGPIKYIFEKPSLSGRI 1261
                 E+R   +E      C+ +     +  +M ++      K  PIKYIFEKPSLSGRI
Sbjct: 1201 KFTDCETRYSQVE---KTCCALAWVARRLRQYMLYYTTWLISKMDPIKYIFEKPSLSGRI 1260

Query: 1262 ARWQVLLSEYDIVYVTQKAIKGSALVDYLAQQPINDYIPVKFDFPDEYISTITASEEILD 1321
            ARWQVLLSEYDIVYVTQKAIKGSAL DYLAQQPINDYIPVKFDFPDEYISTITASEE LD
Sbjct: 1261 ARWQVLLSEYDIVYVTQKAIKGSALADYLAQQPINDYIPVKFDFPDEYISTITASEESLD 1320

Query: 1322 PQTWTMMFDGASNELGHGIGAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVD 1381
            PQTWTMMFDGASNELGH IGAILISPKGELYPLT +LCFDCTHNMAEYEACSMGVQ A+D
Sbjct: 1321 PQTWTMMFDGASNELGHEIGAILISPKGELYPLTTKLCFDCTHNMAEYEACSMGVQAAID 1376

Query: 1382 MKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVA 1441
            MKVKK KVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVA
Sbjct: 1381 MKVKKFKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVA 1376

Query: 1442 DALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPDSNPWFHDIKQYIKSKEYPPN 1465
            DALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPD NPWFHDIKQYIKSKEY PN
Sbjct: 1441 DALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPDGNPWFHDIKQYIKSKEYQPN 1376

BLAST of Moc04g25290 vs. NCBI nr
Match: XP_022150030.1 (LOW QUALITY PROTEIN: uncharacterized protein LOC111018303 [Momordica charantia])

HSP 1 Score: 1441.4 bits (3730), Expect = 0.0e+00
Identity = 823/1335 (61.65%), Postives = 847/1335 (63.45%), Query Frame = 0

Query: 555  KGINIVEDILFVEGSSDSLKPKLLTVFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPW 614
            K  N+VEDIL  EGSSDS+KPK LT+FYREKPDA SCSRKPITI +PAPFEYKSSKAVPW
Sbjct: 21   KRTNVVEDILVAEGSSDSIKPKRLTIFYREKPDAPSCSRKPITITVPAPFEYKSSKAVPW 80

Query: 615  KYECKVTVGQDISFPSLPVDNITGVG---------------------------------- 674
            KYECKVTVGQD+S PSLPVDNITGVG                                  
Sbjct: 81   KYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLLKRVNETTSEKNKEKASEKK 140

Query: 675  ------------------------EETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLS 734
                                    EETQEFLKLVKQ+EYKVIEQLGRTPAKISILSLLLS
Sbjct: 141  KEKVEEDKKGKAKLHEDVHDELVEEETQEFLKLVKQNEYKVIEQLGRTPAKISILSLLLS 200

Query: 735  SEAHQNTLLEALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHIS 794
            SEAH+N LLEALKQAFVSQDITVDNLSNVVGNI  SS ITFTDEEIPPEGTGHTKALHIS
Sbjct: 201  SEAHRNALLEALKQAFVSQDITVDNLSNVVGNIMASSCITFTDEEIPPEGTGHTKALHIS 260

Query: 795  VKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEI 854
            VKCKNFLIAKVLV NGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGAR+AV GDIEI
Sbjct: 261  VKCKNFLIAKVLVGNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARNAVVGDIEI 320

Query: 855  PIQIGPCTFDITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQE 914
            PIQIG CTFDITFQVMDITS YSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQE
Sbjct: 321  PIQIGLCTFDITFQVMDITSAYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQE 380

Query: 915  DILVSRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDK 974
            DILVSRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKP+LLE AFK +NGSLDK
Sbjct: 381  DILVSRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPRLLEAAFKVNNGSLDK 440

Query: 975  LLRMAKNTKKFGLGYKPSRGDIIRVRSLEKAKRLLRFENEELDYPRRTVPPLSHSFRKVG 1034
            LLRMAKNT++FGLGYKP+RGDIIRVRS+EKAKRL RFEN E DY RRTVPPLSHS R  G
Sbjct: 441  LLRMAKNTRRFGLGYKPNRGDIIRVRSMEKAKRLSRFENGERDYSRRTVPPLSHSLRSAG 500

Query: 1035 TIHQEYDESSVVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDN 1094
            TIHQEYDESSV AAVT+EREQVEPFVYPCPDGF+LSNWSV            NTEIECDN
Sbjct: 501  TIHQEYDESSVAAAVTEEREQVEPFVYPCPDGFKLSNWSV------------NTEIECDN 560

Query: 1095 DSKYELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETINLGSQAEAKEIK 1154
            DSKYELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTET+NLGSQAEAKEIK
Sbjct: 561  DSKYELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETLNLGSQAEAKEIK 620

Query: 1155 IGTHMSSESRKKLIELLHDL------------GCSTSKTIH------------------- 1214
            IGTHMSSESRKKLIELLH+             G  T   +H                   
Sbjct: 621  IGTHMSSESRKKLIELLHEYADVFAWSYQDMPGLDTDIVVHKLPTNPKFKPVRQKLRKMR 680

Query: 1215 ------------------------------------------------------------ 1274
                                                                        
Sbjct: 681  PDMLIKIKDEVRKQIDAGFLTVSNYPEWVANIVPVPKKNGQVRMCVDYRDLNRASPKDNF 740

Query: 1275 ------------------------------------------------------------ 1334
                                                                        
Sbjct: 741  PLPHIDVLVDNTAWFSTFSFMDGFSGYNQIKMALEDREKTTFITLWGTFCYKVMSFGLKN 800

Query: 1335 ---------VVLYH--------------MAHFKDG------------------------- 1394
                     V L+H              +A  K G                         
Sbjct: 801  AGATYQRAMVTLFHDLMHKEIEVYVDDMIAKSKQGEEHTTILRKLFDRLRKFKLKLNPNK 860

Query: 1395 ------------------------------------------------------------ 1454
                                                                        
Sbjct: 861  CIFGATTRKLLGFVVSQEGIKVDPDKVKGFLGRLNYIARFISHLTATCEPIFKLLHKNND 920

Query: 1455 ------------------------------------------------------------ 1465
                                                                        
Sbjct: 921  GVWSEDCQASFDKIKQYLQDPPILVPPTPGRPLILYLTVTENSIGCVLGQHDDSGRKEQA 980

BLAST of Moc04g25290 vs. ExPASy Swiss-Prot
Match: Q9HSF6 (Ribonuclease HI OS=Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) OX=64091 GN=rnhA PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-10
Identity = 44/123 (35.77%), Postives = 62/123 (50.41%), Query Frame = 0

Query: 1220 FDGAS--NELGHGIGAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVDMKVKK 1279
            FDGAS  N     +G +L+S  G +           T+N AEY+A    ++ A D     
Sbjct: 74   FDGASRGNPGPAAVGWVLVSGDGGIVAEGGDTIGRATNNQAEYDALIAALEAAADFGFDD 133

Query: 1280 LKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVADALAT 1339
            +++ GDS LV  QL G W+T D  L   +    EL   FD+ S  ++PR  N+ ADALA 
Sbjct: 134  IELRGDSQLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERADALAN 193

Query: 1340 LAV 1341
             A+
Sbjct: 194  EAL 196

BLAST of Moc04g25290 vs. ExPASy Swiss-Prot
Match: P9WLH5 (Bifunctional protein Rv2228c OS=Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv) OX=83332 GN=Rv2228c PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.5e-07
Identity = 34/111 (30.63%), Postives = 54/111 (48.65%), Query Frame = 0

Query: 1254 THNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELS 1313
            T+N+AEY     G+  AV +   +  V  DS LV+ Q+ G W+ +   LL        L+
Sbjct: 44   TNNVAEYRGLIAGLDDAVKLGATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALA 103

Query: 1314 QEFDEISFDYLPRENNQVADALATLAVMFNLE-LNEDVRPIKVGRRDVPAS 1364
             +F  I+++++PR  N  AD LA  A+    +    D  P K+   + P S
Sbjct: 104  SQFRRINYEWVPRARNTYADRLANDAMDAAAQSAAADADPAKIVATESPTS 154

BLAST of Moc04g25290 vs. ExPASy Swiss-Prot
Match: P9WLH4 (Uncharacterized protein MT2287 OS=Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh) OX=83331 GN=MT2287 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.5e-07
Identity = 34/111 (30.63%), Postives = 54/111 (48.65%), Query Frame = 0

Query: 1254 THNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELS 1313
            T+N+AEY     G+  AV +   +  V  DS LV+ Q+ G W+ +   LL        L+
Sbjct: 44   TNNVAEYRGLIAGLDDAVKLGATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALA 103

Query: 1314 QEFDEISFDYLPRENNQVADALATLAVMFNLE-LNEDVRPIKVGRRDVPAS 1364
             +F  I+++++PR  N  AD LA  A+    +    D  P K+   + P S
Sbjct: 104  SQFRRINYEWVPRARNTYADRLANDAMDAAAQSAAADADPAKIVATESPTS 154

BLAST of Moc04g25290 vs. ExPASy Swiss-Prot
Match: P64956 (Uncharacterized protein Mb2253c OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=BQ2027_MB2253C PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 5.5e-07
Identity = 34/111 (30.63%), Postives = 54/111 (48.65%), Query Frame = 0

Query: 1254 THNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELS 1313
            T+N+AEY     G+  AV +   +  V  DS LV+ Q+ G W+ +   LL        L+
Sbjct: 44   TNNVAEYRGLIAGLDDAVKLGATEAAVLMDSKLVVEQMSGRWKVKHPDLLKLYVQAQALA 103

Query: 1314 QEFDEISFDYLPRENNQVADALATLAVMFNLE-LNEDVRPIKVGRRDVPAS 1364
             +F  I+++++PR  N  AD LA  A+    +    D  P K+   + P S
Sbjct: 104  SQFRRINYEWVPRARNTYADRLANDAMDAAAQSAAADADPAKIVATESPTS 154

BLAST of Moc04g25290 vs. ExPASy Swiss-Prot
Match: F9VN79 (Ribonuclease HI OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=rnhA PE=1 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.1e-04
Identity = 26/86 (30.23%), Postives = 51/86 (59.30%), Query Frame = 0

Query: 1254 THNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELS 1313
            T+N+AEY      ++T + + +    + GDS LVI Q+ GE++ +  +++P  +   EL 
Sbjct: 47   TNNVAEYSGLICLMETMLRLGISSPIIKGDSQLVIKQMNGEYKVKAKRIIPLYEKAIELK 106

Query: 1314 QEFDEISFDYLPRENNQVADALATLA 1340
            ++ +  +  ++PRE N+ AD L+ +A
Sbjct: 107  KKLN-ATLIWVPREENKEADRLSRVA 131

BLAST of Moc04g25290 vs. ExPASy TrEMBL
Match: A0A6J1D099 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111016200 PE=4 SV=1)

HSP 1 Score: 2337.0 bits (6055), Expect = 0.0e+00
Identity = 1292/1940 (66.60%), Postives = 1317/1940 (67.89%), Query Frame = 0

Query: 71   MPQYTTYNPLYDVPVGQYPHPFVKGAQQIPTNIIFREPEQMMSPPTVLYLGGLPAKTDLV 130
            MPQYTTYNPLYDVPVGQY HPFVKGAQQIPTNIIFREPEQMMSPPTVL LG L AKTD V
Sbjct: 1    MPQYTTYNPLYDVPVGQYLHPFVKGAQQIPTNIIFREPEQMMSPPTVLNLGDLLAKTDPV 60

Query: 131  GQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGLVILPKF-------------- 190
            GQNAPSNEKFEVL+ERLRAIE TDVFGNIDASQLC VSGLVI PK               
Sbjct: 61   GQNAPSNEKFEVLKERLRAIERTDVFGNIDASQLCSVSGLVIPPKLKVPEFEKYNGSSCP 120

Query: 191  ---------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQLDSSHVGSWKNQADSFLKQYK 250
                     KMAAYVQNDKLLIHCFQDSLS PASRWYMQLDSSHVGSWKN ADSFLKQYK
Sbjct: 121  KNHLXMYCRKMAAYVQNDKLLIHCFQDSLSGPASRWYMQLDSSHVGSWKNLADSFLKQYK 180

Query: 251  HNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPPLTDKELSAMFINTLKHPFYD 310
            HNIDMAPDRLDLQRMEKKST+SFKEYAQRWRDT AQ+QPPL DKELSAMFINTLKHPFYD
Sbjct: 181  HNIDMAPDRLDLQRMEKKSTKSFKEYAQRWRDTAAQVQPPLIDKELSAMFINTLKHPFYD 240

Query: 311  RMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAKKASHSKKKEGEVQMVGADRH 370
            RMIGSASTNFSDIMTIGERIEYGVRHGRITST DEPLAAKKASHSKKKEGEVQMVGADRH
Sbjct: 241  RMIGSASTNFSDIMTIGERIEYGVRHGRITSTTDEPLAAKKASHSKKKEGEVQMVGADRH 300

Query: 371  SWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYASQNFRPPA---------SQN 430
            SWKQQPY RTP+Y+PYYYPTPYGYNQPFVNNATSHY PYASQNFRPPA         SQN
Sbjct: 301  SWKQQPYRRTPQYSPYYYPTPYGYNQPFVNNATSHYYPYASQNFRPPASQNFQLTPTSQN 360

Query: 431  FQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY 490
            FQPRGQQHNTFYTQGQQ NRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY
Sbjct: 361  FQPRGQQHNTFYTQGQQNNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY 420

Query: 491  PRW---------------------------VQALIKVGWLNFKKENGPDVSKNPLPNHQN 550
            PRW                           VQALIK GW NFKKENG DVSK  L NHQN
Sbjct: 421  PRWYDANARCDYHAGAIXHSTENCTXLKYRVQALIKAGWXNFKKENGXDVSKXXLXNHQN 480

Query: 551  VQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFH 610
            VQINAIECQ IESKSKVA+ITTPM ELFEILLGSGY+SVEYLCP  KYKGYDESLTC FH
Sbjct: 481  VQINAIECQGIESKSKVADITTPMXELFEILLGSGYISVEYLCP--KYKGYDESLTCXFH 540

Query: 611  AGAKG--------------------------------INIVEDILFVEGSSDSLKPKLLT 670
             GAKG                                 N+VEDIL  EGSSDSLKPK LT
Sbjct: 541  XGAKGHSLEQCNXFRMKVQELLDSKILTXANSHXKKXTNVVEDILVAEGSSDSLKPKPLT 600

Query: 671  VFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECKVTVGQDISFPSLPVDNITGV 730
            +FYREKPDA SCSRKP  I +P PFEYKSSKAVPWKYECKVTVGQD+S PSLPVDNITGV
Sbjct: 601  IFYREKPDAPSCSRKPXXITVPXPFEYKSSKAVPWKYECKVTVGQDVSSPSLPVDNITGV 660

Query: 731  G----------------------------------------------------------- 790
            G                                                           
Sbjct: 661  GGLTRTGRCYTPDSLLKRVNETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDARDELVEA 720

Query: 791  --------------EETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAHQNTLLE 850
                          EETQEFLKLVKQSEYKVIEQLGRTPA ISILSLLLSSEAHQN LLE
Sbjct: 721  IVVKDVSPKQPMSEEETQEFLKLVKQSEYKVIEQLGRTPANISILSLLLSSEAHQNALLE 780

Query: 851  ALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHISVKCKNFLIAK 910
            ALKQAFVSQDITVDNLSNVVGNIT SSSI+FTDEEIPPEGTGHTKALHISVKCKNFLIAK
Sbjct: 781  ALKQAFVSQDITVDNLSNVVGNITASSSISFTDEEIPPEGTGHTKALHISVKCKNFLIAK 840

Query: 911  VLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEIPIQIGPCTFD 970
            VLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAV GDIEIPIQIGPCTFD
Sbjct: 841  VLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQIGPCTFD 900

Query: 971  ITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILVSRLASM 1030
            ITFQVMDITS YSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILVSR ASM
Sbjct: 901  ITFQVMDITSAYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILVSRFASM 960

Query: 1031 PYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDKLLRMAKNTKK 1090
             YVE AEEAFESSFQSFEIANATTLHGKFGRPKP+LLETAFKGDNGSLDKLLRMAKNTKK
Sbjct: 961  SYVEVAEEAFESSFQSFEIANATTLHGKFGRPKPRLLETAFKGDNGSLDKLLRMAKNTKK 1020

Query: 1091 FGLGYKPSRGDIIRVRSLEKAKRLLRFENEELDYPRRTVPPLSHSFRKVGTIHQEYDESS 1150
            FGLGYKPSRGDIIRVRSLEKAKRL RFENEE DYPRR VPPL+HSFR  GTIHQEYDESS
Sbjct: 1021 FGLGYKPSRGDIIRVRSLEKAKRLSRFENEERDYPRRIVPPLTHSFRSAGTIHQEYDESS 1080

Query: 1151 VVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDNDSKYELDTPI 1210
            VVAAVT+EREQV PFVY CPDGFELSNWSVI+LPSFVNNKSNNTEIECDNDSKYELDTPI
Sbjct: 1081 VVAAVTEEREQVGPFVYLCPDGFELSNWSVIKLPSFVNNKSNNTEIECDNDSKYELDTPI 1140

Query: 1211 YNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETINLGSQAEAKEIKIGTHMSSESR 1270
            Y IESDEEIDDEPSAELLRMLEEEEKMLGPHEELTET+NLGSQAEAKEIKIGTHMSSESR
Sbjct: 1141 YIIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETLNLGSQAEAKEIKIGTHMSSESR 1200

Query: 1271 KKLIELLHD------------LGCSTSKTIH----------------------------- 1330
            KKLIELLH+            LG  T   +H                             
Sbjct: 1201 KKLIELLHEYADVFAWSYXDMLGLDTDIVVHKLPINPEFKPMRQKLRKMRPDMLIKIKNE 1260

Query: 1331 ------------------------------------------------------------ 1390
                                                                        
Sbjct: 1261 VRKQIDAGFLTVSNYPEWVANIVPVPKKNGQVRMCVDYRDLNRASPKDNFPLPHIDVLVD 1320

Query: 1391 -----------------------------------------------------------V 1450
                                                                       V
Sbjct: 1321 NTAGFSTFSFMDGFSGYNQIKMAPEDREKTTFITLWGTFCYKVMSFGLKNAGATYQRAMV 1380

Query: 1451 VLYH--------------MAHFKDG----------------------------------- 1465
             L+H              +A  K G                                   
Sbjct: 1381 TLFHDLMHKEIEVYVDDMIAKSKQGEEHTTILRKLFDRLRKFKLKLNSNKCIFGATTGKL 1440

BLAST of Moc04g25290 vs. ExPASy TrEMBL
Match: A0A6J1CNY7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111013372 PE=4 SV=1)

HSP 1 Score: 1979.9 bits (5128), Expect = 0.0e+00
Identity = 1140/1886 (60.45%), Postives = 1163/1886 (61.66%), Query Frame = 0

Query: 121  GGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGLVILPKF---- 180
            GGLPAKTD VGQNAPSNEKFEVL+ERLRAIEGTDVFGNIDASQLCLVS LVI PKF    
Sbjct: 62   GGLPAKTDPVGQNAPSNEKFEVLKERLRAIEGTDVFGNIDASQLCLVSRLVIPPKFKVPE 121

Query: 181  -------------------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQLDSSHVGSWKN 240
                               KMAAYVQNDKLLIHCFQDSLS PASRWYMQLDSSHVGSWKN
Sbjct: 122  FEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSSPASRWYMQLDSSHVGSWKN 181

Query: 241  QADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPPLTDKELSAMF 300
             ADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDT AQ+QPPLTDKELS MF
Sbjct: 182  LADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTAAQVQPPLTDKELSXMF 241

Query: 301  INTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAKKASHSKKKEG 360
            INTLKHPFYDRM+GSASTNFSDIM IGERIEYGVRHGRITSTADEPLAAKK SHSKKKEG
Sbjct: 242  INTLKHPFYDRMVGSASTNFSDIMAIGERIEYGVRHGRITSTADEPLAAKKTSHSKKKEG 301

Query: 361  EVQMVGADRHSWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYASQNFRPPASQ 420
            E                                                           
Sbjct: 302  E----------------------------------------------------------- 361

Query: 421  NFQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPP 480
                                                            LA VPVDPIQPP
Sbjct: 362  ------------------------------------------------LAHVPVDPIQPP 421

Query: 481  YPRW---------------------------VQALIKVGWLNFKKENGPDVSKNPLPNHQ 540
            YPRW                           VQALIK GWLNFKKENGPDVS NPLPNH 
Sbjct: 422  YPRWCDANARCDYHTGAIGHSIENCTALKYRVQALIKAGWLNFKKENGPDVSNNPLPNHX 481

Query: 541  NVQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPF 600
            NVQINAIECQ IESKSKVA+ITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPF
Sbjct: 482  NVQINAIECQEIESKSKVADITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPF 541

Query: 601  HAGA--------------------------------KGINIVEDI-----LFVEGSSDSL 660
            HAGA                                KGIN+VED+        EGSSD+L
Sbjct: 542  HAGAKGHALEQCNSFRMIVQELLDSKILTVANSHQKKGINVVEDVSVAEGSIAEGSSDAL 601

Query: 661  KPKLLTVFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECKVTVGQDISFPSLPV 720
            KPK LT+FY EKPDA +CSRKPITI +PAPFEYKSSKAVPWKYECKVTVGQD+S P LPV
Sbjct: 602  KPKRLTIFYSEKPDAPNCSRKPITITVPAPFEYKSSKAVPWKYECKVTVGQDVSSPPLPV 661

Query: 721  DNITGVG----------------------------------------------------- 780
            DNITGVG                                                     
Sbjct: 662  DNITGVGGLTXTGRCYTPDSLLKRVSETTSEKNKEKASEKKKEKVEEDKKGKAKLHEDVH 721

Query: 781  --------------------EETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAH 840
                                EE QEFLKLVKQSEYKV EQLGRTPAKISILSLLLSSEAH
Sbjct: 722  DELVEAIVVKDVSPKQHVFEEEIQEFLKLVKQSEYKVTEQLGRTPAKISILSLLLSSEAH 781

Query: 841  QNTLLEALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHISVKCK 900
            +NTLLE LKQAFVSQDITVDNLSNVVGNIT SSSITFTDEEIPPEGTGHTKALHISVKCK
Sbjct: 782  RNTLLEXLKQAFVSQDITVDNLSNVVGNITASSSITFTDEEIPPEGTGHTKALHISVKCK 841

Query: 901  NFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEIPIQI 960
            NFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAV GDIEIPIQI
Sbjct: 842  NFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVVGDIEIPIQI 901

Query: 961  GPCTFDITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILV 1020
            GPCTFDITFQVMDITSTYSFLLGR WIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILV
Sbjct: 902  GPCTFDITFQVMDITSTYSFLLGRLWIHSAGAVPSTLHQKIKFAVDQKLVIISGQEDILV 961

Query: 1021 SRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDKLLRM 1080
            SRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKP+LLETAFKGDN SLDKLLRM
Sbjct: 962  SRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPRLLETAFKGDNESLDKLLRM 1021

Query: 1081 AKNTKKFGLGYKPSRGDIIRVRSLEKAKRLLRFENEELDYPRRTVPPLSHSFRKVGTIHQ 1140
            AKNTKKFGLGYKPSRGDIIRVRSLEKAKRL RFENEE DYPRRTVPPLSHSFR  GTIHQ
Sbjct: 1022 AKNTKKFGLGYKPSRGDIIRVRSLEKAKRLSRFENEERDYPRRTVPPLSHSFRSAGTIHQ 1081

Query: 1141 EYDESSVVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDNDSKY 1200
            EYD SSVVAAVT+EREQV PFVYPCPDGFELSNWSV            NTEIECDNDSKY
Sbjct: 1082 EYDGSSVVAAVTEEREQVRPFVYPCPDGFELSNWSV------------NTEIECDNDSKY 1141

Query: 1201 ELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETINLGSQAEAKEIKIGTH 1260
            ELDTPIYNIESD+EIDDEPSAELLRMLEEEEKMLGPHEELTET+NLGSQAEAKEIKIGTH
Sbjct: 1142 ELDTPIYNIESDKEIDDEPSAELLRMLEEEEKMLGPHEELTETLNLGSQAEAKEIKIGTH 1201

Query: 1261 MSSESRKKLIELLHDL------------GCSTSKTIH----------------------- 1320
            MSSESRKKLIELLH+             G  T   +H                       
Sbjct: 1202 MSSESRKKLIELLHEYADVFAWSYQDMPGLDTDIVVHKLQINPKFKPVRQKLRKMRPDML 1261

Query: 1321 ------------------------------------------------------------ 1380
                                                                        
Sbjct: 1262 IKIKDEVRKQIDAGFLTISNYPEWVANIVPVPKKNGQVRMCVDYRDLNRASPKDNFPLPH 1321

Query: 1381 ------------------------------------------------------------ 1440
                                                                        
Sbjct: 1322 IDVLVDNTAGFSTFSFMDGFSGYNQIKMAPEDREKTTFITLWGTFYYKVMXFGLKNAGAT 1381

Query: 1441 -----VVLYH--------------MAHFKDG----------------------------- 1465
                 V L+H              +A  K G                             
Sbjct: 1382 YQRAMVTLFHDLMHKEIEVYVDDMIAKSKQGEKHTTILRKLFDRLRKFKLKLNPNKCIFG 1441

BLAST of Moc04g25290 vs. ExPASy TrEMBL
Match: A0A6J1E2J7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111025431 PE=4 SV=1)

HSP 1 Score: 1908.3 bits (4942), Expect = 0.0e+00
Identity = 1056/1561 (67.65%), Postives = 1125/1561 (72.07%), Query Frame = 0

Query: 111  MMSPPTVLYLGGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGL 170
            MMSPPTVL LGGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGT VFGNIDASQLCLVSGL
Sbjct: 1    MMSPPTVLNLGGLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTYVFGNIDASQLCLVSGL 60

Query: 171  VILPKF-----------------------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQL 230
            VI PKF                       KMAAYVQNDKLLIHCFQDSLS PASRWYMQL
Sbjct: 61   VIPPKFKVPEFEKYDGSSCPKNHLIMYCRKMAAYVQNDKLLIHCFQDSLSGPASRWYMQL 120

Query: 231  DSSHVGSWKNQADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPP 290
            DSS+VGSWKN ADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDT AQ+QPP
Sbjct: 121  DSSNVGSWKNLADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTAAQVQPP 180

Query: 291  LTDKELSAMFINTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAK 350
            LTDKELSAMFINTLKHPFYDRMIG+ASTNFSDIMTIGERIEYGVRHGRITST DEPLAAK
Sbjct: 181  LTDKELSAMFINTLKHPFYDRMIGNASTNFSDIMTIGERIEYGVRHGRITSTVDEPLAAK 240

Query: 351  KASHSKKKEGEVQMVGADRHSWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYA 410
            KASHSKKKEGEVQMVGADRHSWKQQPY RTPRYTPYYYPTPYGYNQPFVNNATSHYSPY 
Sbjct: 241  KASHSKKKEGEVQMVGADRHSWKQQPYSRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYT 300

Query: 411  SQNFRP---------PASQNFQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLP 470
             QNFRP         PASQNFQPRGQQHNT YTQ QQTNRGARKQTQFDPIPMTYTELLP
Sbjct: 301  FQNFRPPASQNFQPTPASQNFQPRGQQHNTLYTQEQQTNRGARKQTQFDPIPMTYTELLP 360

Query: 471  QLFQNNQLAPVPVDPIQPPYPRW---------------------------VQALIKVGWL 530
            QLFQNNQLAPVPVDPIQPPYPRW                           VQALIK GWL
Sbjct: 361  QLFQNNQLAPVPVDPIQPPYPRWYDTNARCDYHAGAIGHSTENCTALKYRVQALIKAGWL 420

Query: 531  NFKKENGPDVSKNPLPNHQNVQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVE 590
            NFKKENGPDVSKNPLPNHQNVQINAIECQ IESKSKVA+I TPM ELFEILLGSGYVSVE
Sbjct: 421  NFKKENGPDVSKNPLPNHQNVQINAIECQEIESKSKVADIRTPMVELFEILLGSGYVSVE 480

Query: 591  YLCPNLKYKGYDESLTCPFHAGA--------------------------------KGINI 650
            YLCPNLKYKGYDESLTCPFHAGA                                KGINI
Sbjct: 481  YLCPNLKYKGYDESLTCPFHAGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINI 540

Query: 651  VEDILFVEGSSDSLKPKLLTVFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECK 710
            VED+   EGSSD+LKPK LT+FY EKP+A +CSRKPITI +PAPFEYKSSKAVPWKY+CK
Sbjct: 541  VEDVSVAEGSSDALKPKCLTIFYSEKPNAPNCSRKPITITVPAPFEYKSSKAVPWKYQCK 600

Query: 711  VTVGQDISFPSLPVDNITGVG--------------------------------------- 770
            VTVGQD+S P LP+DNITGVG                                       
Sbjct: 601  VTVGQDVSSPPLPIDNITGVGGLTRTGRCYTPDSLLKCVNETTSEKNKEKASEKKKEKVE 660

Query: 771  ----------------------------------EETQEFLKLVKQSEYKVIEQLGRTPA 830
                                              EETQE LKLVKQSEYKVIEQLGRTPA
Sbjct: 661  EDKKGKAKLHEDVHDELVEAIVVKDVSPKQPMSEEETQEILKLVKQSEYKVIEQLGRTPA 720

Query: 831  KISILSLLLSSEAHQNTLLEALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEG 890
            KISILSLLLSSEAH+N LLEALKQAFVSQDITVDNLSNVVGNI+ +SSITFTDEEIPPEG
Sbjct: 721  KISILSLLLSSEAHRNALLEALKQAFVSQDITVDNLSNVVGNISXASSITFTDEEIPPEG 780

Query: 891  TGHTKALHISVKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGA 950
            TGHTKALHIS+KCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGA
Sbjct: 781  TGHTKALHISIKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGA 840

Query: 951  RSAVFGDIEIPIQIGPCTFDITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVD 1010
            RSAV GDIEIPIQIGPCTFDITFQVMDITS YSFLLGRPWIHSAGAVPSTLHQKIKFAVD
Sbjct: 841  RSAVVGDIEIPIQIGPCTFDITFQVMDITSAYSFLLGRPWIHSAGAVPSTLHQKIKFAVD 900

Query: 1011 QKLVIISGQEDILVSRLASMPYVEAAEE--------AFESSFQSF-------EIANATTL 1070
            Q  V              S+P+++   +        +F   F  +       E    TTL
Sbjct: 901  QN-VDYRDLNRASPKDNFSLPHIDVLVDNTTGFSTFSFMDGFSGYNQIKMAPEDREKTTL 960

Query: 1071 HGKFGRPKPQLLETAFKGDNGSLDKLL------RMAKNTKKF--GLGYKPSRGDIIRVRS 1130
               +G    +++    K    +  + +       M K  + +   +  K  +G+      
Sbjct: 961  ITLWGTFCYKVMPFGLKNAGATYQRAMVTLFHDLMHKEIEVYVDDMIAKSKQGEEHTTIL 1020

Query: 1131 LEKAKRLLRFE---NEELDYPRRTVPPLSHSFRKVGTIHQEYDESSVVAAVTKEREQVEP 1190
             +   RL +F+   N        T   L         I  + D+   +  +   + Q E 
Sbjct: 1021 RKLFDRLRKFKLKLNPNKCIFGATTGKLLGFVVSQEDIKVDLDKVKAILEMPPPQTQKEV 1080

Query: 1191 FVYPCPDGF---ELSNWSVIELPSFVNNKSNNTEI---EC----DNDSKYELDTPIYNIE 1250
              +     +    +S+ +    P F   + NN  +   +C    D   +Y  D PI    
Sbjct: 1081 REFLGRLNYIARLISHLTATCEPIFKLLRKNNDGVWSEDCQAAFDKIKQYLQDPPIL--- 1140

Query: 1251 SDEEIDDEPSAELLRMLEEEEK----MLGPHEELTETINLGSQAEAKEIKIGTHMSSESR 1310
                +   P   L+  L   E     +LG H++       G + +A           E+R
Sbjct: 1141 ----VPPTPGRPLILYLTVTENSMGCVLGQHDD------SGRKEQAIYYLSKKFTDCETR 1200

Query: 1311 KKLIE-LLHDLGCSTSKTIHVVLYHMAHF--KDGPIKYIFEKPSLSGRIARWQVLLSEYD 1370
               +E     L  +T +    +LY+      K  PIKYIFEKPSLSGRIARWQVLLSEYD
Sbjct: 1201 YSQVEKTCCALAWATRRLRQYMLYYTTWLISKMDPIKYIFEKPSLSGRIARWQVLLSEYD 1260

Query: 1371 IVYVTQKAIKGSALVDYLAQQPINDYIPVKFDFPDEYISTITASEEILDPQTWTMMFDGA 1430
            IVYVT+KAIKGSAL DYLAQQPINDYIPVKFDFPDEYISTITASEE LDPQTWTMMFDGA
Sbjct: 1261 IVYVTRKAIKGSALADYLAQQPINDYIPVKFDFPDEYISTITASEESLDPQTWTMMFDGA 1320

Query: 1431 SNELGHGIGAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVDMKVKKLKVFGD 1465
            SNELGHGIG ILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQ AVDMKVKKLKVFGD
Sbjct: 1321 SNELGHGIGGILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQAAVDMKVKKLKVFGD 1380

BLAST of Moc04g25290 vs. ExPASy TrEMBL
Match: A0A6J1DZ90 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111024415 PE=4 SV=1)

HSP 1 Score: 1450.6 bits (3754), Expect = 0.0e+00
Identity = 872/1572 (55.47%), Postives = 970/1572 (61.70%), Query Frame = 0

Query: 2    MEDQKAEQEKTRKDIEELREKLDAILLALEKGKAAPDPATSSNPVHEPQETPPYPPGYGP 61
            MEDQKAEQEKTRKDIEELREKLD I L LEKGKA  DPATSSNP+HEPQETPPYPPGYGP
Sbjct: 1    MEDQKAEQEKTRKDIEELREKLDXIXLXLEKGKATADPATSSNPIHEPQETPPYPPGYGP 60

Query: 62   PLRPVAEGFMPQYTTYNPLYDVPVGQYPHPFVKGAQQIPTNIIFREPEQMMSPPTVLYLG 121
            PLRPVAEGFMPQYTTYNPLYDVP+GQYPH FVKGAQQIPTNIIFREPEQMMSPPTV  LG
Sbjct: 61   PLRPVAEGFMPQYTTYNPLYDVPIGQYPHTFVKGAQQIPTNIIFREPEQMMSPPTVXNLG 120

Query: 122  GLPAKTDLVGQNAPSNEKFEVLEERLRAIEGTDVFGNIDASQLCLVSGLVILPKF----- 181
            G PAKTD V QNA S EK EVLEERLRAIEGTDVFGNIDASQLCLVSGLVI PKF     
Sbjct: 121  GXPAKTDPVXQNAXSXEKXEVLEERLRAIEGTDVFGNIDASQLCLVSGLVIPPKFKVPEF 180

Query: 182  ------------------KMAAYVQNDKLLIHCFQDSLSDPASRWYMQLDSSHVGSWKNQ 241
                              KM AYVQN KLLIHCFQDSL   ASRWYMQLDSSHVGSWKN 
Sbjct: 181  EKYDGSSCPKNHLIMYCRKMXAYVQNXKLLIHCFQDSLXGXASRWYMQLDSSHVGSWKNL 240

Query: 242  ADSFLKQYKHNIDMAPDRLDLQRMEKKSTESFKEYAQRWRDTTAQLQPPLTDKELSAMFI 301
            ADSFLKQYKHNIDMAPDRLDLQRMEK STESFKEYAQRWRDT AQ+QPPLTDKELSAMFI
Sbjct: 241  ADSFLKQYKHNIDMAPDRLDLQRMEKNSTESFKEYAQRWRDTAAQVQPPLTDKELSAMFI 300

Query: 302  NTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHGRITSTADEPLAAKKASHSKKKEGE 361
            NTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRH RITSTADEPLAAKKASHSKKKEGE
Sbjct: 301  NTLKHPFYDRMIGSASTNFSDIMTIGERIEYGVRHKRITSTADEPLAAKKASHSKKKEGE 360

Query: 362  VQMVGADRHSWKQQPYGRTPRYTPYYYPTPYGYNQPFVNNATSHYSPYASQNFRPPASQN 421
                                                                        
Sbjct: 361  ------------------------------------------------------------ 420

Query: 422  FQPRGQQHNTFYTQGQQTNRGARKQTQFDPIPMTYTELLPQLFQNNQLAPVPVDPIQPPY 481
                                                           L PVPVDPIQP Y
Sbjct: 421  -----------------------------------------------LTPVPVDPIQPLY 480

Query: 482  PRW---------------------------VQALIKVGWLNFKKENGPDVSKNPLPNHQN 541
            PRW                           VQAL+K GWLNFKKEN PDVSKNPL NHQN
Sbjct: 481  PRWYDANARCDYHAGAIGHSTENCTALKYRVQALLKAGWLNFKKENEPDVSKNPLSNHQN 540

Query: 542  VQINAIECQRIESKSKVAEITTPMEELFEILLGSGYVSVEYLCPNLKYKGYDESLTCPFH 601
            VQINAIECQ IESKSKVA+I TP EELFEILLGSGYVSVEYLCPNLKYK YDESLTCPFH
Sbjct: 541  VQINAIECQGIESKSKVADIRTPKEELFEILLGSGYVSVEYLCPNLKYKEYDESLTCPFH 600

Query: 602  AGA--------------------------------KGINIVEDILFVEGSSDSLKPKLLT 661
            AGA                                KGIN+VED+   EGSSD+LKPK LT
Sbjct: 601  AGAKGHSLEQCNSFRMKVQELLDSKILTVANSHQKKGINVVEDVSVAEGSSDALKPKRLT 660

Query: 662  VFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPWKYECKVTVGQDISFPSLPVDNITGV 721
            +FY EKPDA SCS+KPITI +PAPFEYKSSKAVPWKY+CKVTVGQD+S P LPVDNIT V
Sbjct: 661  IFYSEKPDAPSCSQKPITITVPAPFEYKSSKAVPWKYQCKVTVGQDVSSPPLPVDNITEV 720

Query: 722  GEETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLSSEAHQNTLLEALKQ-----AFVS 781
            G+       L  Q+E K I+           +   +SSE+ +  L+E L +     A+  
Sbjct: 721  GD-------LGSQAEAKEIK-----------IGTHMSSESRKK-LIELLHEYADVFAWSY 780

Query: 782  QDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHISVKCKNFLIAKVLVDNGSS 841
            QD+   +   VV        ++   E  P           + +K K+  + K +     +
Sbjct: 781  QDMPGLDTDIVV------HKLSINPEFKPVRQKLWKMRPDMLIKIKD-EVRKQIDAGFLT 840

Query: 842  LNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEIPIQIGPCTFDITFQVMDI 901
            ++  P      +PV   + +   + V   D  R++   +  +P        D+       
Sbjct: 841  VSNYPEWVANIVPVPKKNGQ-VRMCVDYRDLNRASPKDNFPLP------HIDVLVDNTAG 900

Query: 902  TSTYSFLLGRPWIHSAGAVPSTLHQKIKF----AVDQKLVIISGQEDILVSRLASMPYVE 961
             ST+SF+ G    +     P    +K  F          V+  G +++  +   +M  V 
Sbjct: 901  FSTFSFMGGFSGXNXIKMAPED-REKTTFITLWGTFCYKVMPFGLKNVGATYQRAM--VT 960

Query: 962  AAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDKL----LRMAKNTKK 1021
               +      + +   +      K G     +L   F       D+L    L++  N   
Sbjct: 961  LFHDLMHKEIEVY--VDDMIAKSKQGEEHTTILRKLF-------DRLRKFKLKLNPNKCI 1020

Query: 1022 FGLGYKPSRGDIIRVRSL----EKAKRLLRFENEELDYPRRTVPPLSHSFRKVGTIHQEY 1081
            FG       G ++    +    +K K +L             +PP        G + +  
Sbjct: 1021 FGATTGKLLGFVVSQEGIKVDPDKVKAILE------------MPPPQTQKEVRGFLGRLN 1080

Query: 1082 DESSVVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDNDSKYEL 1141
              +  ++ +T   E +   +    DG     W            S + +   +   +Y  
Sbjct: 1081 YIARFISHLTATCEPIFKLLRKNNDGV----W------------SEDCQAAFNKIKQYLQ 1140

Query: 1142 DTPIYNIESDEEIDDEPSAELLRMLEEEEK----MLGPHEELTETINLGSQAEAKEIKIG 1201
            D PI        +   P   L+  L   E     +LG H++       G + +A      
Sbjct: 1141 DPPIL-------VPPTPGRPLILYLTVTENSMGCVLGQHDD------SGRKEQAIYYLSK 1200

Query: 1202 THMSSESRKKLIELLHDLGCSTSKTIHVVLYHMAHF------KDGPIKYIFEKPSLSGRI 1261
                 E+R   +E      C+ +     +  +M ++      K  PIKYIFEKPSLSGRI
Sbjct: 1201 KFTDCETRYSQVE---KTCCALAWVARRLRQYMLYYTTWLISKMDPIKYIFEKPSLSGRI 1260

Query: 1262 ARWQVLLSEYDIVYVTQKAIKGSALVDYLAQQPINDYIPVKFDFPDEYISTITASEEILD 1321
            ARWQVLLSEYDIVYVTQKAIKGSAL DYLAQQPINDYIPVKFDFPDEYISTITASEE LD
Sbjct: 1261 ARWQVLLSEYDIVYVTQKAIKGSALADYLAQQPINDYIPVKFDFPDEYISTITASEESLD 1320

Query: 1322 PQTWTMMFDGASNELGHGIGAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVD 1381
            PQTWTMMFDGASNELGH IGAILISPKGELYPLT +LCFDCTHNMAEYEACSMGVQ A+D
Sbjct: 1321 PQTWTMMFDGASNELGHEIGAILISPKGELYPLTTKLCFDCTHNMAEYEACSMGVQAAID 1376

Query: 1382 MKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVA 1441
            MKVKK KVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVA
Sbjct: 1381 MKVKKFKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVA 1376

Query: 1442 DALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPDSNPWFHDIKQYIKSKEYPPN 1465
            DALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPD NPWFHDIKQYIKSKEY PN
Sbjct: 1441 DALATLAVMFNLELNEDVRPIKVGRRDVPASCMSIEEEPDGNPWFHDIKQYIKSKEYQPN 1376

BLAST of Moc04g25290 vs. ExPASy TrEMBL
Match: A0A6J1D7C7 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111018303 PE=4 SV=1)

HSP 1 Score: 1441.4 bits (3730), Expect = 0.0e+00
Identity = 823/1335 (61.65%), Postives = 847/1335 (63.45%), Query Frame = 0

Query: 555  KGINIVEDILFVEGSSDSLKPKLLTVFYREKPDASSCSRKPITIRIPAPFEYKSSKAVPW 614
            K  N+VEDIL  EGSSDS+KPK LT+FYREKPDA SCSRKPITI +PAPFEYKSSKAVPW
Sbjct: 21   KRTNVVEDILVAEGSSDSIKPKRLTIFYREKPDAPSCSRKPITITVPAPFEYKSSKAVPW 80

Query: 615  KYECKVTVGQDISFPSLPVDNITGVG---------------------------------- 674
            KYECKVTVGQD+S PSLPVDNITGVG                                  
Sbjct: 81   KYECKVTVGQDVSSPSLPVDNITGVGGLTRTGRCYTPDSLLKRVNETTSEKNKEKASEKK 140

Query: 675  ------------------------EETQEFLKLVKQSEYKVIEQLGRTPAKISILSLLLS 734
                                    EETQEFLKLVKQ+EYKVIEQLGRTPAKISILSLLLS
Sbjct: 141  KEKVEEDKKGKAKLHEDVHDELVEEETQEFLKLVKQNEYKVIEQLGRTPAKISILSLLLS 200

Query: 735  SEAHQNTLLEALKQAFVSQDITVDNLSNVVGNITTSSSITFTDEEIPPEGTGHTKALHIS 794
            SEAH+N LLEALKQAFVSQDITVDNLSNVVGNI  SS ITFTDEEIPPEGTGHTKALHIS
Sbjct: 201  SEAHRNALLEALKQAFVSQDITVDNLSNVVGNIMASSCITFTDEEIPPEGTGHTKALHIS 260

Query: 795  VKCKNFLIAKVLVDNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARSAVFGDIEI 854
            VKCKNFLIAKVLV NGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGAR+AV GDIEI
Sbjct: 261  VKCKNFLIAKVLVGNGSSLNIMPRSTLEKLPVDMSHMRPSTVIVRAFDGARNAVVGDIEI 320

Query: 855  PIQIGPCTFDITFQVMDITSTYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQE 914
            PIQIG CTFDITFQVMDITS YSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQE
Sbjct: 321  PIQIGLCTFDITFQVMDITSAYSFLLGRPWIHSAGAVPSTLHQKIKFAVDQKLVIISGQE 380

Query: 915  DILVSRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPQLLETAFKGDNGSLDK 974
            DILVSRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKP+LLE AFK +NGSLDK
Sbjct: 381  DILVSRLASMPYVEAAEEAFESSFQSFEIANATTLHGKFGRPKPRLLEAAFKVNNGSLDK 440

Query: 975  LLRMAKNTKKFGLGYKPSRGDIIRVRSLEKAKRLLRFENEELDYPRRTVPPLSHSFRKVG 1034
            LLRMAKNT++FGLGYKP+RGDIIRVRS+EKAKRL RFEN E DY RRTVPPLSHS R  G
Sbjct: 441  LLRMAKNTRRFGLGYKPNRGDIIRVRSMEKAKRLSRFENGERDYSRRTVPPLSHSLRSAG 500

Query: 1035 TIHQEYDESSVVAAVTKEREQVEPFVYPCPDGFELSNWSVIELPSFVNNKSNNTEIECDN 1094
            TIHQEYDESSV AAVT+EREQVEPFVYPCPDGF+LSNWSV            NTEIECDN
Sbjct: 501  TIHQEYDESSVAAAVTEEREQVEPFVYPCPDGFKLSNWSV------------NTEIECDN 560

Query: 1095 DSKYELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETINLGSQAEAKEIK 1154
            DSKYELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTET+NLGSQAEAKEIK
Sbjct: 561  DSKYELDTPIYNIESDEEIDDEPSAELLRMLEEEEKMLGPHEELTETLNLGSQAEAKEIK 620

Query: 1155 IGTHMSSESRKKLIELLHDL------------GCSTSKTIH------------------- 1214
            IGTHMSSESRKKLIELLH+             G  T   +H                   
Sbjct: 621  IGTHMSSESRKKLIELLHEYADVFAWSYQDMPGLDTDIVVHKLPTNPKFKPVRQKLRKMR 680

Query: 1215 ------------------------------------------------------------ 1274
                                                                        
Sbjct: 681  PDMLIKIKDEVRKQIDAGFLTVSNYPEWVANIVPVPKKNGQVRMCVDYRDLNRASPKDNF 740

Query: 1275 ------------------------------------------------------------ 1334
                                                                        
Sbjct: 741  PLPHIDVLVDNTAWFSTFSFMDGFSGYNQIKMALEDREKTTFITLWGTFCYKVMSFGLKN 800

Query: 1335 ---------VVLYH--------------MAHFKDG------------------------- 1394
                     V L+H              +A  K G                         
Sbjct: 801  AGATYQRAMVTLFHDLMHKEIEVYVDDMIAKSKQGEEHTTILRKLFDRLRKFKLKLNPNK 860

Query: 1395 ------------------------------------------------------------ 1454
                                                                        
Sbjct: 861  CIFGATTRKLLGFVVSQEGIKVDPDKVKGFLGRLNYIARFISHLTATCEPIFKLLHKNND 920

Query: 1455 ------------------------------------------------------------ 1465
                                                                        
Sbjct: 921  GVWSEDCQASFDKIKQYLQDPPILVPPTPGRPLILYLTVTENSIGCVLGQHDDSGRKEQA 980

BLAST of Moc04g25290 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.6e-12
Identity = 48/169 (28.40%), Postives = 76/169 (44.97%), Query Frame = 0

Query: 1174 GSALVDYLAQQPINDYIPVKFDFPDEYISTITASEEILDPQTWTMMFDGAS--NELGHGI 1233
            GS      +  P    + ++ D      S++     I    + T+ FDGAS  N    G 
Sbjct: 113  GSGESGSFSPSPPQKQLKIENDMLRRIPSSLLTRTPIRQNDSCTIEFDGASKGNPGKAGA 172

Query: 1234 GAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQL 1293
            GA+L +    +         + T+N+AEY A  +G+++A+D   K + V GDS LV  Q+
Sbjct: 173  GAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKGFKNVHVLGDSMLVCMQV 232

Query: 1294 RGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVADALATLAV 1341
            +G W+T   K+    +   EL   F      ++ RE N  AD  A  A+
Sbjct: 233  QGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADKQANSAI 281

BLAST of Moc04g25290 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 2.6e-12
Identity = 48/169 (28.40%), Postives = 76/169 (44.97%), Query Frame = 0

Query: 1174 GSALVDYLAQQPINDYIPVKFDFPDEYISTITASEEILDPQTWTMMFDGAS--NELGHGI 1233
            GS      +  P    + ++ D      S++     I    + T+ FDGAS  N    G 
Sbjct: 113  GSGESGSFSPSPPQKQLKIENDMLRRIPSSLLTRTPIRQNDSCTIEFDGASKGNPGKAGA 172

Query: 1234 GAILISPKGELYPLTARLCFDCTHNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQL 1293
            GA+L +    +         + T+N+AEY A  +G+++A+D   K + V GDS LV  Q+
Sbjct: 173  GAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDKGFKNVHVLGDSMLVCMQV 232

Query: 1294 RGEWETRDVKLLPYKQLITELSQEFDEISFDYLPRENNQVADALATLAV 1341
            +G W+T   K+    +   EL   F      ++ RE N  AD  A  A+
Sbjct: 233  QGAWKTNHPKMAELCKQAKELMNSFKTFDIKHIAREKNSEADKQANSAI 281

BLAST of Moc04g25290 vs. TAIR 10
Match: AT1G24090.1 (RNase H family protein )

HSP 1 Score: 62.4 bits (150), Expect = 3.5e-09
Identity = 47/146 (32.19%), Postives = 69/146 (47.26%), Query Frame = 0

Query: 1197 PDEYISTITASEEILDPQTWTMMFDGAS--NELGHGIGAILISPKGELYPLTARLCFDCT 1256
            P E +S +  S  I D +T  + FDGAS  N    G  A+L +  G L     +     T
Sbjct: 199  PLEKLSKVEPSAYISD-ETCFIEFDGASKGNPGLSGAAAVLKTEDGSLICRVRQGLGIAT 258

Query: 1257 HNMAEYEACSMGVQTAVDMKVKKLKVFGDSTLVIHQLRGEWETRDVKLLPYKQLITELSQ 1316
            +N AEY A  +G++ A++   K +KV GDS LV  Q++G+W+     L    +    L  
Sbjct: 259  NNAAEYHALILGLKYAIEKGYKNIKVKGDSKLVCMQIKGQWKVNHEVLAKLHKEAKLLCN 318

Query: 1317 EFDEISFDYLPRENNQVADALATLAV 1341
            +       ++ R  N  AD  A LAV
Sbjct: 319  KCVSFEISHVLRNLNADADEQANLAV 343

BLAST of Moc04g25290 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 4.0e-05
Identity = 40/138 (28.99%), Postives = 65/138 (47.10%), Query Frame = 0

Query: 1213 PQTWTMMFDGASNELGH---GIGAILISPKGELYPLTARLCFDCTHNM--AEYEACSMGV 1272
            P  W      A+ +L +   GIG IL +  G +  + AR     T N+  AE EA    V
Sbjct: 141  PYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGAR-ALPRTKNVLEAELEALRWAV 200

Query: 1273 QTAVDMKVKKLKVFGDSTLVIHQLRGE--WETRDVKLLPYKQLITELSQEFDEISFDYLP 1332
             T      K++    D+  +++ L  +  W T    L P  + I +L   F+E+ F++ P
Sbjct: 201  LTMSRFNYKRIIFESDAQALVNLLNSDDFWPT----LQPALEDIQQLLHHFEEVKFEFTP 260

Query: 1333 RENNQVADALATLAVMFN 1344
            R  N+VAD +A  ++ F+
Sbjct: 261  RGGNKVADRIARESISFS 273