Moc04g25070 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc04g25070 vs. NCBI nr
Match: XP_022152854.1 (uncharacterized protein LOC111020479 [Momordica charantia])

HSP 1 Score: 955.7 bits (2469), Expect = 2.5e-274
Identity = 526/753 (69.85%), Postives = 573/753 (76.10%), Query Frame = 0

Query: 5   MVQPANSTNTADRRTLAASDAHQREVGAAVVEGQGHDGLATEPLRRSARITAPVLPPAHP 64
           MVQPANSTNTADRR LAA+  HQREVGA VVEGQGH+ L TEPL RSARIT PVLPPAH 
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAH- 60

Query: 65  PRTSKATRGRGGTSKKGARGPAPAPTSENLDALQREMEAMRTKMRSMEEMYNEMILVAGA 124
           P+ SKA                                         E  YN        
Sbjct: 61  PKPSKA-----------------------------------------ESSYN-------- 120

Query: 125 GSRSENRVTRVGIREQRGSHLGPVEEEHPEDNESEGHTRQREDLRVITREEFNQLRGQLD 184
                                 P+                     VITREEF+QL+ + D
Sbjct: 121 ----------------------PITP------------------GVITREEFDQLKSKFD 180

Query: 185 AKVEALKAKCEQKEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEV 244
           A+VEALKA+CE+KE S +DGDLGE  F+SD+LEA IPPKFK PT+KPYDGSKDPKDYVEV
Sbjct: 181 AQVEALKARCEKKESSFDDGDLGELSFSSDILEALIPPKFKTPTMKPYDGSKDPKDYVEV 240

Query: 245 FEGLMDFQAASDAIKCRAFQIALTGSARLWYRRLPARLISTYSQLRREFLAQFSSRHYDK 304
           FE LMDFQAA+DAIKC AFQIALTGSARLWYRRLPARLISTYSQLR+EF++QFSSRHYD+
Sbjct: 241 FESLMDFQAATDAIKCCAFQIALTGSARLWYRRLPARLISTYSQLRKEFISQFSSRHYDR 300

Query: 305 KTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKIGEEA 364
           KT THLATIRQKEGETLREYVTRF EEQLKVAHCSDDSAMCYFLTGLADE LTVK+ EEA
Sbjct: 301 KTPTHLATIRQKEGETLREYVTRFPEEQLKVAHCSDDSAMCYFLTGLADETLTVKLREEA 360

Query: 365 PATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGKDIEKADPKSKDKG-SFSSGRA 424
           PATFAEVLQK KKVI+GQELLRTKTGRPE+ I +GR+GKD  KAD KS+DKG S SS R 
Sbjct: 361 PATFAEVLQKTKKVIDGQELLRTKTGRPEKNIDQGRAGKDKGKADSKSRDKGPSSSSSRV 420

Query: 425 EYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKLLKRPEKLRGAPERRNKDKYC 484
           +YRR+ +   +SRPYE +T TTIPI +ILTNIEE+GMEKLLKRPEKLRG PE+RN DKYC
Sbjct: 421 DYRRSNSSHNQSRPYEHYTPTTIPIFEILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYC 480

Query: 485 RFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEEQKRSRTPPRRTDRP 544
           RFHR+H HNTS+  ELKRQIEDLIQDGYFKKFVGKPR++S EKKEE+KR RTPPRR DRP
Sbjct: 481 RFHRDHGHNTSNYWELKRQIEDLIQDGYFKKFVGKPRSNSVEKKEERKRLRTPPRRDDRP 540

Query: 545 AVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFHGADLEEVHLPHNDAL 604
           AVIN             K+KELAR ARREVCIIREQRPT  I F+ ADLE VHLPHNDAL
Sbjct: 541 AVIN-------------KKKELAREARREVCIIREQRPTSSIAFNHADLEGVHLPHNDAL 600

Query: 605 VIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQLKKSPTPLVGFSGESVIPEGY 664
           VIAPLID V+VRR+LVDGGASANILSL TY+ALGWTRSQLKKSPTPLVGFSGES+  EG 
Sbjct: 601 VIAPLIDLVLVRRILVDGGASANILSLSTYLALGWTRSQLKKSPTPLVGFSGESISLEGC 650

Query: 665 IDLPVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRALPSTLHQVLKYSTPNGV 724
           IDLPV++ QD TQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRA+PSTLHQVLKYST NGV
Sbjct: 661 IDLPVSIRQDDTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTLNGV 650

Query: 725 GTVRGEPTASRECYASALKGSSVCALETLASRD 757
           GTVRGE   SRECYAS  K SSVCALE    RD
Sbjct: 721 GTVRGELKTSRECYASVPKRSSVCALEEQTIRD 650

BLAST of Moc04g25070 vs. NCBI nr
Match: XP_022137317.1 (uncharacterized protein LOC111008813 [Momordica charantia])

HSP 1 Score: 953.0 bits (2462), Expect = 1.6e-273
Identity = 488/515 (94.76%), Postives = 499/515 (96.89%), Query Frame = 0

Query: 170 VITREEFNQLRGQLDAKVEALKAKCEQKEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTV 229
           VITREEF+QLRGQLDA+VEALKAKCEQKEG LNDGDLGES FTSDVLEAPIPPKFKAPTV
Sbjct: 16  VITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPIPPKFKAPTV 75

Query: 230 KPYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLWYRRLPARLISTYSQL 289
           KPYDGSKDPKDYVEVFE LMDFQAASDAIKCRAF+IALTGSARLWYRRLPA  ISTYSQL
Sbjct: 76  KPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWYRRLPAXSISTYSQL 135

Query: 290 RREFLAQFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT 349
           RREFLA FSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT
Sbjct: 136 RREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT 195

Query: 350 GLADEALTVKIGEEAPATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGKDIEKAD 409
           GLADEALTVK+GEEAPATFAEVLQKAKKVI+GQELLRTKTGRPERKIGRGRSGKDIE AD
Sbjct: 196 GLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKIGRGRSGKDIENAD 255

Query: 410 PKSKDKGSFSSGRAEYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKLLKRPEK 469
           PKSKDKGSFSSGRAEYRRAENG TRSRPYERFT TTIPIS+ILTNIEESGMEKLLKRPEK
Sbjct: 256 PKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKLLKRPEK 315

Query: 470 LRGAPERRNKDKYCRFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEE 529
           LRGAPERR+KDKYCRFHREH HNTSD  ELKRQIE+LIQDGYFKKFVGKPRTSSAEKKEE
Sbjct: 316 LRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKFVGKPRTSSAEKKEE 375

Query: 530 QKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFHG 589
           +KRSRTPPRRTDRPAVINTIFGGPSGGQSG KRKELARAARREVCIIREQRPTCPITF G
Sbjct: 376 RKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCIIREQRPTCPITFDG 435

Query: 590 ADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQLKKSPTP 649
           ADLEEVHLPHNDALVIAPLIDHVVV RVLVDGG SANILSLPTY+ALGWTRSQLKKSPTP
Sbjct: 436 ADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLALGWTRSQLKKSPTP 495

Query: 650 LVGFSGESVIPEGYIDLPVTLGQDQTQVTQMAEFV 685
           LVGFSGESVIPEG+IDLPVTLGQDQTQVTQMAEFV
Sbjct: 496 LVGFSGESVIPEGFIDLPVTLGQDQTQVTQMAEFV 530

BLAST of Moc04g25070 vs. NCBI nr
Match: XP_022150760.1 (uncharacterized protein LOC111018823 [Momordica charantia])

HSP 1 Score: 934.9 bits (2415), Expect = 4.6e-268
Identity = 504/614 (82.08%), Postives = 520/614 (84.69%), Query Frame = 0

Query: 170 VITREEFNQLRGQLDAKVEALKAKCEQKEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTV 229
           VITREEF+QLRG+L+A+VEALKAKCEQKEG LNDGDLGES FTSDVLE        APTV
Sbjct: 19  VITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLE--------APTV 78

Query: 230 KPYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLWYRRLPARLISTYSQL 289
           K YDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLW               
Sbjct: 79  KSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLW--------------- 138

Query: 290 RREFLAQFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT 349
                                                 FQE+QLKVA  SDDSAMCYFLT
Sbjct: 139 --------------------------------------FQEDQLKVAQSSDDSAMCYFLT 198

Query: 350 GLADEALTVKIGEEAPATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGKDIEKAD 409
           GLADEALTVK+G+EAPATFAEVLQKAKKVI+GQELLRTKTGRPER I RGRSGKD EKAD
Sbjct: 199 GLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELLRTKTGRPERGIDRGRSGKD-EKAD 258

Query: 410 PKSKDKGSFSSGRAEYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKLLKRPEK 469
            KSKDKGSFSSGRAE+RRA NG TRSRPYERFT TTIPIS+ILTNIEESGMEKLLKRPEK
Sbjct: 259 LKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTIPISEILTNIEESGMEKLLKRPEK 318

Query: 470 LRGAPERRNKDKYCRFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEE 529
           LRGAPERRNKDKYCRFHREH HNTSD  ELKRQIEDLIQD YFKKFVGKPRTSSAEKKEE
Sbjct: 319 LRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDLIQDDYFKKFVGKPRTSSAEKKEE 378

Query: 530 QKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFHG 589
           +K SRTP RR DRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITF  
Sbjct: 379 RKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFDS 438

Query: 590 ADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQLKKSPTP 649
           ADLEEVHLPHNDALVIAPLIDHVVVRRVLVD G SANI+SL TY+ALGWTRSQLKKS TP
Sbjct: 439 ADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSANIVSLLTYLALGWTRSQLKKSTTP 498

Query: 650 LVGFSGESVIPEGYIDLPVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRALPS 709
           LVGFS ESVIPEG IDLPVTLG DQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRA+PS
Sbjct: 499 LVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRAIPS 558

Query: 710 TLHQVLKYSTPNGVGTVRGEPTASRECYASALKGSSVCALETLASRDGTLEFEADLPRRE 769
           TLHQVLKYSTPNGVG VRGE  ASRECYASALKGSSVCALETL SRDGTLEF+A+LPRRE
Sbjct: 559 TLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSVCALETLVSRDGTLEFKANLPRRE 570

Query: 770 FAAPTEGLELVPLL 784
           FAAPTE LELVPLL
Sbjct: 619 FAAPTEELELVPLL 570

BLAST of Moc04g25070 vs. NCBI nr
Match: XP_022152110.1 (uncharacterized protein LOC111019899 [Momordica charantia])

HSP 1 Score: 781.2 bits (2016), Expect = 8.5e-222
Identity = 402/444 (90.54%), Postives = 417/444 (93.92%), Query Frame = 0

Query: 344 MCYFLTGLADEALTVKIGEEAPATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGK 403
           MCYFLTGLADEALTVK+ EEAPATFAEVLQKAKKVI+GQELLRT       KIG+GRSGK
Sbjct: 1   MCYFLTGLADEALTVKLVEEAPATFAEVLQKAKKVIDGQELLRT-------KIGQGRSGK 60

Query: 404 DIEKADPKSKDKGSFSSGRAEYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKL 463
           D+E  DPKSKDKGSFS+GRAEYRRAENG TRSRPYERFT TTIPIS+ILTNIEESGMEKL
Sbjct: 61  DMENTDPKSKDKGSFSNGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 120

Query: 464 LKRPEKLRGAPERRNKDKYCRFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSS 523
           LKRPEKLRGAPERR+KDKYCRFHREH HNTSD  ELK QIEDLIQDGYFKKFVGKPRTSS
Sbjct: 121 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSS 180

Query: 524 AEKKEEQKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTC 583
           AEKKEE+KRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRK+LARAARREVCIIREQRPTC
Sbjct: 181 AEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTC 240

Query: 584 PITFHGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQL 643
           PITF  ADL EVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTY+ALGWTRSQL
Sbjct: 241 PITFDXADLXEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQL 300

Query: 644 KKSPTPLVGFSGESVIPEGYIDLPVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHS 703
           KKSPTPLVGFSGESV+PEG IDLPVTLGQDQT+VTQMAEFVV+DGRSAYNAIFGRPIIHS
Sbjct: 301 KKSPTPLVGFSGESVVPEGCIDLPVTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHS 360

Query: 704 FRALPSTLHQVLKYSTPNGVGTVRGEPTASRECYASALKGSSVCALETLASRDGTLEFEA 763
           FRA+PSTLHQVLKYSTPNGVGTVRGE TASRECYAS LKG+SVCALETL SRDGTLEFEA
Sbjct: 361 FRAIPSTLHQVLKYSTPNGVGTVRGEQTASRECYASXLKGTSVCALETLTSRDGTLEFEA 420

Query: 764 DLPRREFAAPTEGLELVPLLSPEK 788
           DLP REFAAP E LELVPLLS EK
Sbjct: 421 DLPXREFAAPXEELELVPLLSXEK 437

BLAST of Moc04g25070 vs. NCBI nr
Match: XP_022150613.1 (uncharacterized protein LOC111018708, partial [Momordica charantia])

HSP 1 Score: 771.2 bits (1990), Expect = 8.8e-219
Identity = 393/422 (93.13%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 197 KEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFQAASD 256
           K+ SLNDGDLGES FTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDF AASD
Sbjct: 44  KDDSLNDGDLGESSFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFHAASD 103

Query: 257 AIKCRAFQIALTGSARLWYRRLPARLISTYSQLRREFLAQFSSRHYDKKTATHLATIRQK 316
           AIKCRAFQIALTGSARLWYRRLPAR ISTYSQLRREFLAQFSSR Y KKT THLATIRQK
Sbjct: 104 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRQYGKKTETHLATIRQK 163

Query: 317 EGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKIGEEAPATFAEVLQKAK 376
           EG TLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVK+GE+AP TFAEVLQKAK
Sbjct: 164 EGGTLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEKAPTTFAEVLQKAK 223

Query: 377 KVINGQELLRTKTGRPERKIGRGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGLTRSR 436
           KVI+GQELLRTKTGRP+RKIGRGRSGKD+E+ADPKSKDKGSFSSGRAEYRRAE+G T+SR
Sbjct: 224 KVIDGQELLRTKTGRPDRKIGRGRSGKDVERADPKSKDKGSFSSGRAEYRRAESGPTKSR 283

Query: 437 PYERFTSTTIPISKILTNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHCHNTSDC 496
           PYERFT TTIPIS+ILTNIEESGMEKLLKRPEKLRGAPERR+KDKYCRFHREH HNTSDC
Sbjct: 284 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 343

Query: 497 LELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEEQKRSRTPPRRTDRPAVINTIFGGPSGG 556
            ELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEE+KRSRTPPRRTDRPAVINTIFGGPSGG
Sbjct: 344 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGG 403

Query: 557 QSGHKRKELARAARREVCIIREQRPTCPITFHGADLEEVHLPHNDALVIAPLIDHVVVRR 616
           QSGHKRKELARAARREVCIIREQ PTCPITF GAD EEVHLPHNDA VIAPLIDHVVVRR
Sbjct: 404 QSGHKRKELARAARREVCIIREQGPTCPITFDGADSEEVHLPHNDARVIAPLIDHVVVRR 463

Query: 617 VL 619
           VL
Sbjct: 464 VL 465

BLAST of Moc04g25070 vs. ExPASy TrEMBL
Match: A0A6J1DHB3 (uncharacterized protein LOC111020479 OS=Momordica charantia OX=3673 GN=LOC111020479 PE=4 SV=1)

HSP 1 Score: 955.7 bits (2469), Expect = 1.2e-274
Identity = 526/753 (69.85%), Postives = 573/753 (76.10%), Query Frame = 0

Query: 5   MVQPANSTNTADRRTLAASDAHQREVGAAVVEGQGHDGLATEPLRRSARITAPVLPPAHP 64
           MVQPANSTNTADRR LAA+  HQREVGA VVEGQGH+ L TEPL RSARIT PVLPPAH 
Sbjct: 1   MVQPANSTNTADRRALAANHGHQREVGAEVVEGQGHEDLGTEPLCRSARITTPVLPPAH- 60

Query: 65  PRTSKATRGRGGTSKKGARGPAPAPTSENLDALQREMEAMRTKMRSMEEMYNEMILVAGA 124
           P+ SKA                                         E  YN        
Sbjct: 61  PKPSKA-----------------------------------------ESSYN-------- 120

Query: 125 GSRSENRVTRVGIREQRGSHLGPVEEEHPEDNESEGHTRQREDLRVITREEFNQLRGQLD 184
                                 P+                     VITREEF+QL+ + D
Sbjct: 121 ----------------------PITP------------------GVITREEFDQLKSKFD 180

Query: 185 AKVEALKAKCEQKEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEV 244
           A+VEALKA+CE+KE S +DGDLGE  F+SD+LEA IPPKFK PT+KPYDGSKDPKDYVEV
Sbjct: 181 AQVEALKARCEKKESSFDDGDLGELSFSSDILEALIPPKFKTPTMKPYDGSKDPKDYVEV 240

Query: 245 FEGLMDFQAASDAIKCRAFQIALTGSARLWYRRLPARLISTYSQLRREFLAQFSSRHYDK 304
           FE LMDFQAA+DAIKC AFQIALTGSARLWYRRLPARLISTYSQLR+EF++QFSSRHYD+
Sbjct: 241 FESLMDFQAATDAIKCCAFQIALTGSARLWYRRLPARLISTYSQLRKEFISQFSSRHYDR 300

Query: 305 KTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKIGEEA 364
           KT THLATIRQKEGETLREYVTRF EEQLKVAHCSDDSAMCYFLTGLADE LTVK+ EEA
Sbjct: 301 KTPTHLATIRQKEGETLREYVTRFPEEQLKVAHCSDDSAMCYFLTGLADETLTVKLREEA 360

Query: 365 PATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGKDIEKADPKSKDKG-SFSSGRA 424
           PATFAEVLQK KKVI+GQELLRTKTGRPE+ I +GR+GKD  KAD KS+DKG S SS R 
Sbjct: 361 PATFAEVLQKTKKVIDGQELLRTKTGRPEKNIDQGRAGKDKGKADSKSRDKGPSSSSSRV 420

Query: 425 EYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKLLKRPEKLRGAPERRNKDKYC 484
           +YRR+ +   +SRPYE +T TTIPI +ILTNIEE+GMEKLLKRPEKLRG PE+RN DKYC
Sbjct: 421 DYRRSNSSHNQSRPYEHYTPTTIPIFEILTNIEETGMEKLLKRPEKLRGDPEKRNTDKYC 480

Query: 485 RFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEEQKRSRTPPRRTDRP 544
           RFHR+H HNTS+  ELKRQIEDLIQDGYFKKFVGKPR++S EKKEE+KR RTPPRR DRP
Sbjct: 481 RFHRDHGHNTSNYWELKRQIEDLIQDGYFKKFVGKPRSNSVEKKEERKRLRTPPRRDDRP 540

Query: 545 AVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFHGADLEEVHLPHNDAL 604
           AVIN             K+KELAR ARREVCIIREQRPT  I F+ ADLE VHLPHNDAL
Sbjct: 541 AVIN-------------KKKELAREARREVCIIREQRPTSSIAFNHADLEGVHLPHNDAL 600

Query: 605 VIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQLKKSPTPLVGFSGESVIPEGY 664
           VIAPLID V+VRR+LVDGGASANILSL TY+ALGWTRSQLKKSPTPLVGFSGES+  EG 
Sbjct: 601 VIAPLIDLVLVRRILVDGGASANILSLSTYLALGWTRSQLKKSPTPLVGFSGESISLEGC 650

Query: 665 IDLPVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRALPSTLHQVLKYSTPNGV 724
           IDLPV++ QD TQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRA+PSTLHQVLKYST NGV
Sbjct: 661 IDLPVSIRQDDTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRAVPSTLHQVLKYSTLNGV 650

Query: 725 GTVRGEPTASRECYASALKGSSVCALETLASRD 757
           GTVRGE   SRECYAS  K SSVCALE    RD
Sbjct: 721 GTVRGELKTSRECYASVPKRSSVCALEEQTIRD 650

BLAST of Moc04g25070 vs. ExPASy TrEMBL
Match: A0A6J1C7X5 (uncharacterized protein LOC111008813 OS=Momordica charantia OX=3673 GN=LOC111008813 PE=4 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 7.9e-274
Identity = 488/515 (94.76%), Postives = 499/515 (96.89%), Query Frame = 0

Query: 170 VITREEFNQLRGQLDAKVEALKAKCEQKEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTV 229
           VITREEF+QLRGQLDA+VEALKAKCEQKEG LNDGDLGES FTSDVLEAPIPPKFKAPTV
Sbjct: 16  VITREEFDQLRGQLDAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLEAPIPPKFKAPTV 75

Query: 230 KPYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLWYRRLPARLISTYSQL 289
           KPYDGSKDPKDYVEVFE LMDFQAASDAIKCRAF+IALTGSARLWYRRLPA  ISTYSQL
Sbjct: 76  KPYDGSKDPKDYVEVFESLMDFQAASDAIKCRAFRIALTGSARLWYRRLPAXSISTYSQL 135

Query: 290 RREFLAQFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT 349
           RREFLA FSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT
Sbjct: 136 RREFLAXFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT 195

Query: 350 GLADEALTVKIGEEAPATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGKDIEKAD 409
           GLADEALTVK+GEEAPATFAEVLQKAKKVI+GQELLRTKTGRPERKIGRGRSGKDIE AD
Sbjct: 196 GLADEALTVKLGEEAPATFAEVLQKAKKVIDGQELLRTKTGRPERKIGRGRSGKDIENAD 255

Query: 410 PKSKDKGSFSSGRAEYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKLLKRPEK 469
           PKSKDKGSFSSGRAEYRRAENG TRSRPYERFT TTIPIS+ILTNIEESGMEKLLKRPEK
Sbjct: 256 PKSKDKGSFSSGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKLLKRPEK 315

Query: 470 LRGAPERRNKDKYCRFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEE 529
           LRGAPERR+KDKYCRFHREH HNTSD  ELKRQIE+LIQDGYFKKFVGKPRTSSAEKKEE
Sbjct: 316 LRGAPERRSKDKYCRFHREHGHNTSDYWELKRQIENLIQDGYFKKFVGKPRTSSAEKKEE 375

Query: 530 QKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFHG 589
           +KRSRTPPRRTDRPAVINTIFGGPSGGQSG KRKELARAARREVCIIREQRPTCPITF G
Sbjct: 376 RKRSRTPPRRTDRPAVINTIFGGPSGGQSGRKRKELARAARREVCIIREQRPTCPITFDG 435

Query: 590 ADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQLKKSPTP 649
           ADLEEVHLPHNDALVIAPLIDHVVV RVLVDGG SANILSLPTY+ALGWTRSQLKKSPTP
Sbjct: 436 ADLEEVHLPHNDALVIAPLIDHVVVXRVLVDGGTSANILSLPTYLALGWTRSQLKKSPTP 495

Query: 650 LVGFSGESVIPEGYIDLPVTLGQDQTQVTQMAEFV 685
           LVGFSGESVIPEG+IDLPVTLGQDQTQVTQMAEFV
Sbjct: 496 LVGFSGESVIPEGFIDLPVTLGQDQTQVTQMAEFV 530

BLAST of Moc04g25070 vs. ExPASy TrEMBL
Match: A0A6J1D9E1 (uncharacterized protein LOC111018823 OS=Momordica charantia OX=3673 GN=LOC111018823 PE=4 SV=1)

HSP 1 Score: 934.9 bits (2415), Expect = 2.2e-268
Identity = 504/614 (82.08%), Postives = 520/614 (84.69%), Query Frame = 0

Query: 170 VITREEFNQLRGQLDAKVEALKAKCEQKEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTV 229
           VITREEF+QLRG+L+A+VEALKAKCEQKEG LNDGDLGES FTSDVLE        APTV
Sbjct: 19  VITREEFDQLRGKLNAQVEALKAKCEQKEGPLNDGDLGESPFTSDVLE--------APTV 78

Query: 230 KPYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLWYRRLPARLISTYSQL 289
           K YDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLW               
Sbjct: 79  KSYDGSKDPKDYVEVFEGLMDFQAASDAIKCRAFQIALTGSARLW--------------- 138

Query: 290 RREFLAQFSSRHYDKKTATHLATIRQKEGETLREYVTRFQEEQLKVAHCSDDSAMCYFLT 349
                                                 FQE+QLKVA  SDDSAMCYFLT
Sbjct: 139 --------------------------------------FQEDQLKVAQSSDDSAMCYFLT 198

Query: 350 GLADEALTVKIGEEAPATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGKDIEKAD 409
           GLADEALTVK+G+EAPATFAEVLQKAKKVI+GQELLRTKTGRPER I RGRSGKD EKAD
Sbjct: 199 GLADEALTVKLGKEAPATFAEVLQKAKKVIDGQELLRTKTGRPERGIDRGRSGKD-EKAD 258

Query: 410 PKSKDKGSFSSGRAEYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKLLKRPEK 469
            KSKDKGSFSSGRAE+RRA NG TRSRPYERFT TTIPIS+ILTNIEESGMEKLLKRPEK
Sbjct: 259 LKSKDKGSFSSGRAEFRRAVNGPTRSRPYERFTPTTIPISEILTNIEESGMEKLLKRPEK 318

Query: 470 LRGAPERRNKDKYCRFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEE 529
           LRGAPERRNKDKYCRFHREH HNTSD  ELKRQIEDLIQD YFKKFVGKPRTSSAEKKEE
Sbjct: 319 LRGAPERRNKDKYCRFHREHDHNTSDRWELKRQIEDLIQDDYFKKFVGKPRTSSAEKKEE 378

Query: 530 QKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFHG 589
           +K SRTP RR DRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITF  
Sbjct: 379 RKLSRTPLRRIDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTCPITFDS 438

Query: 590 ADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQLKKSPTP 649
           ADLEEVHLPHNDALVIAPLIDHVVVRRVLVD G SANI+SL TY+ALGWTRSQLKKS TP
Sbjct: 439 ADLEEVHLPHNDALVIAPLIDHVVVRRVLVDEGVSANIVSLLTYLALGWTRSQLKKSTTP 498

Query: 650 LVGFSGESVIPEGYIDLPVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRALPS 709
           LVGFS ESVIPEG IDLPVTLG DQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRA+PS
Sbjct: 499 LVGFSRESVIPEGCIDLPVTLGHDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHSFRAIPS 558

Query: 710 TLHQVLKYSTPNGVGTVRGEPTASRECYASALKGSSVCALETLASRDGTLEFEADLPRRE 769
           TLHQVLKYSTPNGVG VRGE  ASRECYASALKGSSVCALETL SRDGTLEF+A+LPRRE
Sbjct: 559 TLHQVLKYSTPNGVGMVRGEQIASRECYASALKGSSVCALETLVSRDGTLEFKANLPRRE 570

Query: 770 FAAPTEGLELVPLL 784
           FAAPTE LELVPLL
Sbjct: 619 FAAPTEELELVPLL 570

BLAST of Moc04g25070 vs. ExPASy TrEMBL
Match: A0A6J1DD03 (uncharacterized protein LOC111019899 OS=Momordica charantia OX=3673 GN=LOC111019899 PE=4 SV=1)

HSP 1 Score: 781.2 bits (2016), Expect = 4.1e-222
Identity = 402/444 (90.54%), Postives = 417/444 (93.92%), Query Frame = 0

Query: 344 MCYFLTGLADEALTVKIGEEAPATFAEVLQKAKKVINGQELLRTKTGRPERKIGRGRSGK 403
           MCYFLTGLADEALTVK+ EEAPATFAEVLQKAKKVI+GQELLRT       KIG+GRSGK
Sbjct: 1   MCYFLTGLADEALTVKLVEEAPATFAEVLQKAKKVIDGQELLRT-------KIGQGRSGK 60

Query: 404 DIEKADPKSKDKGSFSSGRAEYRRAENGLTRSRPYERFTSTTIPISKILTNIEESGMEKL 463
           D+E  DPKSKDKGSFS+GRAEYRRAENG TRSRPYERFT TTIPIS+ILTNIEESGMEKL
Sbjct: 61  DMENTDPKSKDKGSFSNGRAEYRRAENGPTRSRPYERFTPTTIPISEILTNIEESGMEKL 120

Query: 464 LKRPEKLRGAPERRNKDKYCRFHREHCHNTSDCLELKRQIEDLIQDGYFKKFVGKPRTSS 523
           LKRPEKLRGAPERR+KDKYCRFHREH HNTSD  ELK QIEDLIQDGYFKKFVGKPRTSS
Sbjct: 121 LKRPEKLRGAPERRSKDKYCRFHREHGHNTSDYWELKSQIEDLIQDGYFKKFVGKPRTSS 180

Query: 524 AEKKEEQKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKELARAARREVCIIREQRPTC 583
           AEKKEE+KRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRK+LARAARREVCIIREQRPTC
Sbjct: 181 AEKKEERKRSRTPPRRTDRPAVINTIFGGPSGGQSGHKRKKLARAARREVCIIREQRPTC 240

Query: 584 PITFHGADLEEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYIALGWTRSQL 643
           PITF  ADL EVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTY+ALGWTRSQL
Sbjct: 241 PITFDXADLXEVHLPHNDALVIAPLIDHVVVRRVLVDGGASANILSLPTYLALGWTRSQL 300

Query: 644 KKSPTPLVGFSGESVIPEGYIDLPVTLGQDQTQVTQMAEFVVIDGRSAYNAIFGRPIIHS 703
           KKSPTPLVGFSGESV+PEG IDLPVTLGQDQT+VTQMAEFVV+DGRSAYNAIFGRPIIHS
Sbjct: 301 KKSPTPLVGFSGESVVPEGCIDLPVTLGQDQTRVTQMAEFVVVDGRSAYNAIFGRPIIHS 360

Query: 704 FRALPSTLHQVLKYSTPNGVGTVRGEPTASRECYASALKGSSVCALETLASRDGTLEFEA 763
           FRA+PSTLHQVLKYSTPNGVGTVRGE TASRECYAS LKG+SVCALETL SRDGTLEFEA
Sbjct: 361 FRAIPSTLHQVLKYSTPNGVGTVRGEQTASRECYASXLKGTSVCALETLTSRDGTLEFEA 420

Query: 764 DLPRREFAAPTEGLELVPLLSPEK 788
           DLP REFAAP E LELVPLLS EK
Sbjct: 421 DLPXREFAAPXEELELVPLLSXEK 437

BLAST of Moc04g25070 vs. ExPASy TrEMBL
Match: A0A6J1D9W7 (uncharacterized protein LOC111018708 OS=Momordica charantia OX=3673 GN=LOC111018708 PE=4 SV=1)

HSP 1 Score: 771.2 bits (1990), Expect = 4.3e-219
Identity = 393/422 (93.13%), Postives = 405/422 (95.97%), Query Frame = 0

Query: 197 KEGSLNDGDLGESRFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFQAASD 256
           K+ SLNDGDLGES FTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDF AASD
Sbjct: 44  KDDSLNDGDLGESSFTSDVLEAPIPPKFKAPTVKPYDGSKDPKDYVEVFEGLMDFHAASD 103

Query: 257 AIKCRAFQIALTGSARLWYRRLPARLISTYSQLRREFLAQFSSRHYDKKTATHLATIRQK 316
           AIKCRAFQIALTGSARLWYRRLPAR ISTYSQLRREFLAQFSSR Y KKT THLATIRQK
Sbjct: 104 AIKCRAFQIALTGSARLWYRRLPARSISTYSQLRREFLAQFSSRQYGKKTETHLATIRQK 163

Query: 317 EGETLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKIGEEAPATFAEVLQKAK 376
           EG TLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVK+GE+AP TFAEVLQKAK
Sbjct: 164 EGGTLREYVTRFQEEQLKVAHCSDDSAMCYFLTGLADEALTVKLGEKAPTTFAEVLQKAK 223

Query: 377 KVINGQELLRTKTGRPERKIGRGRSGKDIEKADPKSKDKGSFSSGRAEYRRAENGLTRSR 436
           KVI+GQELLRTKTGRP+RKIGRGRSGKD+E+ADPKSKDKGSFSSGRAEYRRAE+G T+SR
Sbjct: 224 KVIDGQELLRTKTGRPDRKIGRGRSGKDVERADPKSKDKGSFSSGRAEYRRAESGPTKSR 283

Query: 437 PYERFTSTTIPISKILTNIEESGMEKLLKRPEKLRGAPERRNKDKYCRFHREHCHNTSDC 496
           PYERFT TTIPIS+ILTNIEESGMEKLLKRPEKLRGAPERR+KDKYCRFHREH HNTSDC
Sbjct: 284 PYERFTPTTIPISEILTNIEESGMEKLLKRPEKLRGAPERRSKDKYCRFHREHGHNTSDC 343

Query: 497 LELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEEQKRSRTPPRRTDRPAVINTIFGGPSGG 556
            ELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEE+KRSRTPPRRTDRPAVINTIFGGPSGG
Sbjct: 344 WELKRQIEDLIQDGYFKKFVGKPRTSSAEKKEERKRSRTPPRRTDRPAVINTIFGGPSGG 403

Query: 557 QSGHKRKELARAARREVCIIREQRPTCPITFHGADLEEVHLPHNDALVIAPLIDHVVVRR 616
           QSGHKRKELARAARREVCIIREQ PTCPITF GAD EEVHLPHNDA VIAPLIDHVVVRR
Sbjct: 404 QSGHKRKELARAARREVCIIREQGPTCPITFDGADSEEVHLPHNDARVIAPLIDHVVVRR 463

Query: 617 VL 619
           VL
Sbjct: 464 VL 465