Moc04g24740 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g24740
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Ribonuclease H
Location	chr4: 17915392 .. 17916636 (-)
RNA-Seq Expression	Moc04g24740
Synteny	Moc04g24740

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGCGGACTTCATTAGGGGCAACTCACCACAAGACCCTAAGGAGCGCCGAAAGTTGGCAAGGCGGGCAGCTCGGTTCGTGGTCCGAGATGAGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAGATGCCTAACCCCCGAAGAGGGCCTGTACGTATTCAGAGAGATCCACGAAGGAGTGTGCGGCAATCACTCAGGCGCCCGGTCGCTGTCAGCCAAAGTAATCCGACAAGGATACTATTGGCCGACCCTCAGCCAGGACGCCAAGAAGTTCGTTAGAACTTGCGACAATTGCCAACGCTACGGAACCATAATCCACCAACCTCCCGAGCTGCTCACCCTCATCTCGGCCCCGTGGCCATTCGCGCAGTGGGGGGTAGATATCATTGGTCCTTTCCCTTTGGGCAAGGGCCAGACCAAGTTTGCTGTGGTTGCTGTGGATTACTTCACCAAGTGAGCCGAGGCCGAAGCGCTCTCCCACATAACGGAATCCAGGGTCACGTCCTTCGTATGGACGAATATCATATGTCGCTTTGGTATACCGCAGGCCATAGTGACAGACAATGGGAAGCAGTTTGACAACGCCAAGTTCAAAGACTTTTGCAGCAAACTTGGCATAAGTTATCTTAGCTCGTCCCCCGCACATCCGCAAGCAAATGGGCAGGTGGAAGCAGTCAACAAGATCATCAAGCGAGGCATCAAACTTAGACTGGACTCCAAGAAAGGCAGGTGGGCCGAGGAGCTACCAGAGGTTCTATGGTCGTACCGGACCACCCAACGAGAGTCGACGGATGAGACCCCGTTCTCCCTGGCCTTCGGCTCCGAAGCTGTAGTCCCGGTTGAGATCGGCATGCCATCTGACAGAGTAGAGCATTACGAGCCTACGACAAATGAGGATGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCGATGGCCCAGCTACGCCTGATGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAACGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCATTTGAGATCAAGGGGATAGTCCGACCTGGGACGTACTTATTGGCCGATCTGAAAGGAGATGTCCTCGCGCACCCGTGGAACGCGGAACACCTGAAGCGTTATTATCCTTGA

mRNA sequence

ATGGATATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGCGGACTTCATTAGGGGCAACTCACCACAAGACCCTAAGGAGCGCCGAAAGTTGGCAAGGCGGGCAGCTCGGTTCGTGGTCCGAGATGAGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAGATGCCTAACCCCCGAAGAGGGCCTAGTAGAGCATTACGAGCCTACGACAAATGAGGATGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCGATGGCCCAGCTACGCCTGATGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAACGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCATTTGAGATCAAGGGGATAGTCCGACCTGGGACGTACTTATTGGCCGATCTGAAAGGAGATGTCCTCGCGCACCCGTGGAACGCGGAACACCTGAAGCGTTATTATCCTTGA

Coding sequence (CDS)

ATGGATATCGGCGCTCCAGAATCCTCATGGATGGACCCGATCGCGGACTTCATTAGGGGCAACTCACCACAAGACCCTAAGGAGCGCCGAAAGTTGGCAAGGCGGGCAGCTCGGTTCGTGGTCCGAGATGAGGCATTGTACCGACGTGGCTTTTCCCTGCCTCTATTGAGATGCCTAACCCCCGAAGAGGGCCTAGTAGAGCATTACGAGCCTACGACAAATGAGGATGAGCTGCTCCTCAACCTCGACTTGTTGGAAGAAAGAAGAGCGATGGCCCAGCTACGCCTGATGGAATATCAGGGCAGAATGGCCAGACATTACAATGCCCGCGTTCGACCTCGGGCCTTTCAGGTCGGACATCTGGTCTTAAGGAGGGTCCAAACGCATGTGGGTGCCCTTGATCCGGCCTGGGAGGGCCCATTTGAGATCAAGGGGATAGTCCGACCTGGGACGTACTTATTGGCCGATCTGAAAGGAGATGTCCTCGCGCACCCGTGGAACGCGGAACACCTGAAGCGTTATTATCCTTGA

Protein sequence

MDIGAPESSWMDPIADFIRGNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCLTPEEGLVEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPRAFQVGHLVLRRVQTHVGALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHPWNAEHLKRYYP

Homology

[Close]

BLAST of Moc04g24740 vs. NCBI nr
Match: XP_022159003.1 (uncharacterized protein LOC111025448 [Momordica charantia])

HSP 1 Score: 176.8 bits (447), Expect = 1.6e-40
Identity = 81/111 (72.97%), Postives = 94/111 (84.68%), Query Frame = 0


Query: 66  VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           +E YEP  NE ELLLNLDLLE +R  AQLRL+E Q RMARHYN R++PR+FQVGHLV  +
Sbjct: 519 IEQYEPLRNEAELLLNLDLLEGKRETAQLRLVECQNRMARHYNTRIQPRSFQVGHLVSSK 578

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHPWNAEHLKRYYP 177
           +Q+HVG LDP+WE  FE+KGIVRPGTY+LADL+  VLAHPWNAEHLKRYYP
Sbjct: 579 IQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc04g24740 vs. NCBI nr
Match: GFY92947.1 (hypothetical protein Acr_08g0013430 [Actinidia rufa])

HSP 1 Score: 153.7 bits (387), Expect = 1.5e-33
Identity = 90/206 (43.69%), Postives = 116/206 (56.31%), Query Frame = 0


Query: 9   SWMDPIADFIR-GNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCLTPEEG--L 68
           +W+D I D++R G  P+D  + R+L  R+ARF + +  LY+R FS PLLRCL PEE   +
Sbjct: 200 TWLDEIIDYLRKGILPKDKLQARRLQYRSARFCIFEGRLYKRSFSGPLLRCLRPEEAKYV 259

Query: 69  VEHY---------EP----------------------TTNEDELLLNLDLLEERRAMAQL 128
            E Y         EP                        NE EL LNLDLL+E+R  A+L
Sbjct: 260 YEKYTKAYAGITPEPDRWQRKLFAKDILADHGTRCGYICNEGELRLNLDLLDEKRESAEL 319

Query: 129 RLMEYQGRMARHYNARVRPRAFQVGHLVLRRV-----QTHVGALDPAWEGPFEIKGIVRP 176
           R   Y+GR+A++YN RV  R+F  G LV RRV         G L P WEGP+++  + RP
Sbjct: 320 RQAAYKGRIAKYYNERVEHRSFLPGDLVWRRVTLSTRDPSAGKLGPTWEGPYKVIKVSRP 379

[Close]

BLAST of Moc04g24740 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 151.8 bits (382), Expect = 5.7e-33
Identity = 69/100 (69.00%), Postives = 87/100 (87.00%), Query Frame = 0


Query: 66  VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           VE+YE   NE+ELLL LDL++E+R  AQLRL EYQ +MARHYN+RVR R+FQ+G+LVLR+
Sbjct: 251 VEYYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARHYNSRVRSRSFQIGYLVLRK 310

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHP 166
           VQ HV  L+P+WEGPFE+KGIVRPGTY+LADL+G +L+HP
Sbjct: 311 VQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc04g24740 vs. NCBI nr
Match: XP_022156070.1 (uncharacterized protein K02A2.6-like [Momordica charantia])

HSP 1 Score: 63.9 bits (154), Expect = 1.6e-06
Identity = 28/54 (51.85%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 11 MDPIADFIRGNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCLTPEEG 65
          MDPI DF+ G    D KE +++AR+ AR+++R+  LYR GFSLPLL+C+  E+G
Sbjct: 1  MDPIKDFLEGKVSTDLKETKRMARKEARYMLRNGTLYRCGFSLPLLKCIFKEDG 54

HSP 2 Score: 150.2 bits (378), Expect = 1.7e-32
Identity = 90/254 (35.43%), Postives = 124/254 (48.82%), Query Frame = 0


Query: 1   MDIGAPESSWMDPIADF-IRGNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCL 60
           M +   + +WMD I +F + G+ P DP + RKL  ++A ++++++ L++RG+SLPLLRCL
Sbjct: 1   MKVQLAQPTWMDRIKNFLVSGSVPTDPSQARKLRCQSAHYLMQEDKLFKRGYSLPLLRCL 60

Query: 61  TPE-----------------------EGL------------------------------- 120
            PE                        GL                               
Sbjct: 61  DPEVASSSPAHLQSNGQIEAVNKIIKRGLKTRLESLKDRWAEELPEVLWSYRTIPRPSTG 120

Query: 121 ------------------------VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQG 176
                                   VE++   TN + LL+NLDLL E+R+ +QLRL EY  
Sbjct: 121 ETPYDLALGSEAVIPVEIGMLTPRVENFNEQTNSEALLVNLDLLAEKRSHSQLRLAEYHN 180

[Close]

BLAST of Moc04g24740 vs. NCBI nr
Match: XP_023876159.1 (uncharacterized protein LOC111988600 [Quercus suber])

HSP 1 Score: 142.5 bits (358), Expect = 3.4e-30
Identity = 78/186 (41.94%), Postives = 107/186 (57.53%), Query Frame = 0


Query: 7    ESSWMDPIADFIR-GNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCLTPEEG- 66
            E +WM PI  +++ G  P+   E RKL  R+AR+++ DE LY+RGFS P LRCL P+E  
Sbjct: 834  EENWMTPIVSYLKDGRLPEGKDEARKLRVRSARYILMDEVLYKRGFSQPYLRCLAPDEAN 893

Query: 67   --LVEHYEPTTNE--------DELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPR 126
              L E +E              ++ LNLDL++E R  A+ R+  Y+  MAR ++A V+PR
Sbjct: 894  YLLREVHEGACGNHSGARSLVHKIRLNLDLIDEVRMDAEQRVARYKNLMARQHDAMVKPR 953

Query: 127  AFQVGHLVLRRVQTHV-----GALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHPWNAE 176
             F +G LVL++V           L P WEGP+ +    RPG+Y L  L    L HPWN E
Sbjct: 954  RFNIGDLVLKKVSLATKNPAHEKLGPNWEGPYRVINSKRPGSYYLEALDRRKLEHPWNVE 1013

[Close]

BLAST of Moc04g24740 vs. ExPASy TrEMBL
Match: A0A6J1DXL9 (uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025448 PE=4 SV=1)

HSP 1 Score: 176.8 bits (447), Expect = 8.0e-41
Identity = 81/111 (72.97%), Postives = 94/111 (84.68%), Query Frame = 0


Query: 66  VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           +E YEP  NE ELLLNLDLLE +R  AQLRL+E Q RMARHYN R++PR+FQVGHLV  +
Sbjct: 519 IEQYEPLRNEAELLLNLDLLEGKRETAQLRLVECQNRMARHYNTRIQPRSFQVGHLVSSK 578

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHPWNAEHLKRYYP 177
           +Q+HVG LDP+WE  FE+KGIVRPGTY+LADL+  VLAHPWNAEHLKRYYP
Sbjct: 579 IQSHVGTLDPSWEELFEVKGIVRPGTYMLADLERSVLAHPWNAEHLKRYYP 629

[Close]

BLAST of Moc04g24740 vs. ExPASy TrEMBL
Match: A0A7J0F4U0 (Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_08g0013430 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 7.2e-34
Identity = 90/206 (43.69%), Postives = 116/206 (56.31%), Query Frame = 0


Query: 9   SWMDPIADFIR-GNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCLTPEEG--L 68
           +W+D I D++R G  P+D  + R+L  R+ARF + +  LY+R FS PLLRCL PEE   +
Sbjct: 200 TWLDEIIDYLRKGILPKDKLQARRLQYRSARFCIFEGRLYKRSFSGPLLRCLRPEEAKYV 259

Query: 69  VEHY---------EP----------------------TTNEDELLLNLDLLEERRAMAQL 128
            E Y         EP                        NE EL LNLDLL+E+R  A+L
Sbjct: 260 YEKYTKAYAGITPEPDRWQRKLFAKDILADHGTRCGYICNEGELRLNLDLLDEKRESAEL 319

Query: 129 RLMEYQGRMARHYNARVRPRAFQVGHLVLRRV-----QTHVGALDPAWEGPFEIKGIVRP 176
           R   Y+GR+A++YN RV  R+F  G LV RRV         G L P WEGP+++  + RP
Sbjct: 320 RQAAYKGRIAKYYNERVEHRSFLPGDLVWRRVTLSTRDPSAGKLGPTWEGPYKVIKVSRP 379

[Close]

BLAST of Moc04g24740 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 151.8 bits (382), Expect = 2.8e-33
Identity = 69/100 (69.00%), Postives = 87/100 (87.00%), Query Frame = 0


Query: 66  VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           VE+YE   NE+ELLL LDL++E+R  AQLRL EYQ +MARHYN+RVR R+FQ+G+LVLR+
Sbjct: 251 VEYYERVRNEEELLLYLDLIDEKREAAQLRLAEYQNQMARHYNSRVRSRSFQIGYLVLRK 310

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHP 166
           VQ HV  L+P+WEGPFE+KGIVRPGTY+LADL+G +L+HP
Sbjct: 311 VQKHVEVLEPSWEGPFEVKGIVRPGTYMLADLEGKLLSHP 350

[Close]

BLAST of Moc04g24740 vs. ExPASy TrEMBL
Match: A0A6J1DP99 (uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023037 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 7.5e-07
Identity = 28/54 (51.85%), Postives = 40/54 (74.07%), Query Frame = 0


Query: 11 MDPIADFIRGNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCLTPEEG 65
          MDPI DF+ G    D KE +++AR+ AR+++R+  LYR GFSLPLL+C+  E+G
Sbjct: 1  MDPIKDFLEGKVSTDLKETKRMARKEARYMLRNGTLYRCGFSLPLLKCIFKEDG 54

HSP 2 Score: 150.2 bits (378), Expect = 8.0e-33
Identity = 90/254 (35.43%), Postives = 124/254 (48.82%), Query Frame = 0


Query: 1   MDIGAPESSWMDPIADF-IRGNSPQDPKERRKLARRAARFVVRDEALYRRGFSLPLLRCL 60
           M +   + +WMD I +F + G+ P DP + RKL  ++A ++++++ L++RG+SLPLLRCL
Sbjct: 1   MKVQLAQPTWMDRIKNFLVSGSVPTDPSQARKLRCQSAHYLMQEDKLFKRGYSLPLLRCL 60

Query: 61  TPE-----------------------EGL------------------------------- 120
            PE                        GL                               
Sbjct: 61  DPEVASSSPAHLQSNGQIEAVNKIIKRGLKTRLESLKDRWAEELPEVLWSYRTIPRPSTG 120

Query: 121 ------------------------VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQG 176
                                   VE++   TN + LL+NLDLL E+R+ +QLRL EY  
Sbjct: 121 ETPYDLALGSEAVIPVEIGMLTPRVENFNEQTNSEALLVNLDLLAEKRSHSQLRLAEYHN 180

[Close]

BLAST of Moc04g24740 vs. ExPASy TrEMBL
Match: A0A6J1DWM2 (Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1)

HSP 1 Score: 141.7 bits (356), Expect = 2.8e-30
Identity = 65/110 (59.09%), Postives = 84/110 (76.36%), Query Frame = 0


Query: 66  VEHYEPTTNEDELLLNLDLLEERRAMAQLRLMEYQGRMARHYNARVRPRAFQVGHLVLRR 125
           VE+++   N + LL+NLDLLEE+R+ +QLRL EYQ RMAR+YNARV+ R F+   LVL++
Sbjct: 735 VENFDEQANSEALLVNLDLLEEKRSHSQLRLAEYQNRMARYYNARVKVRTFKPSDLVLKK 794

Query: 126 VQTHVGALDPAWEGPFEIKGIVRPGTYLLADLKGDVLAHPWNAEHLKRYY 176
           V  HVGAL+P WEGP+ +  I+RP  YLL+DL G  L HPWNAEHL+ YY
Sbjct: 795 VMQHVGALEPNWEGPYRVLKILRPRAYLLSDLNGRQLPHPWNAEHLRVYY 844

The following BLAST results are available for this feature:

BLAST of Moc04g24740 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022159003.1	1.6e-40	72.97	uncharacterized protein LOC111025448 [Momordica charantia]	[more]
GFY92947.1	1.5e-33	43.69	hypothetical protein Acr_08g0013430 [Actinidia rufa]	[more]
XP_022156070.1	5.7e-33	69.00	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]
XP_022156070.1	1.6e-06	51.85	uncharacterized protein K02A2.6-like [Momordica charantia]	[more]
XP_023876159.1	3.4e-30	41.94	uncharacterized protein LOC111988600 [Quercus suber]	[more]

BLAST of Moc04g24740 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc04g24740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DXL9	8.0e-41	72.97	uncharacterized protein LOC111025448 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A7J0F4U0	7.2e-34	43.69	Uncharacterized protein OS=Actinidia rufa OX=165716 GN=Acr_08g0013430 PE=4 SV=1	[more]
A0A6J1DP99	2.8e-33	69.00	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DP99	7.5e-07	51.85	uncharacterized protein K02A2.6-like OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1DWM2	2.8e-30	59.09	Ribonuclease H OS=Momordica charantia OX=3673 GN=LOC111023816 PE=4 SV=1	[more]

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BLAST of Moc04g24740 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 82..102

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g24740.1	Moc04g24740.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015074	DNA integration
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
cellular_component	GO:0030430	host cell cytoplasm
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity