Moc04g24310 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc04g24310 vs. NCBI nr
Match: XP_038907006.1 (aspartyl protease family protein 2 [Benincasa hispida])

HSP 1 Score: 706.8 bits (1823), Expect = 1.2e-199
Identity = 360/450 (80.00%), Postives = 386/450 (85.78%), Query Frame = 0

Query: 10  PFPQLFPLLLLLLFSNATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRN 69
           PF     L   L   NATAA  +LKLPLLH  PFSSPS AL+ D HRLS+LFS     R 
Sbjct: 6   PFFHFIFLFFFLTNFNATAA-DYLKLPLLHKPPFSSPSQALSSDTHRLSLLFS-----RP 65

Query: 70  RNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSS 129
              +KSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCS CRNCSHH PS+
Sbjct: 66  NPTLKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPST 125

Query: 130 AFLPRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDT 189
           AFLPRHSSSFSP HCFDP CRL+PHA   LCNHTR HSPC + YSYADGSLS+GFFSK+T
Sbjct: 126 AFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRFHSPCRFLYSYADGSLSSGFFSKET 185

Query: 190 TTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGN 249
           TTLKTLSG+E  L  LSFGCGFRISGPSVSG+ F+GARGVMGLGRG ISFSSQLGRRFGN
Sbjct: 186 TTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFSGARGVMGLGRGSISFSSQLGRRFGN 245

Query: 250 KFSYCLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAV 309
           KFSYCLMDYTLSPPPTSYLMI G  RS  V+NA++ISYTPLQINPLSPTFYYI + SI V
Sbjct: 246 KFSYCLMDYTLSPPPTSYLMIGGGHRSLPVTNATKISYTPLQINPLSPTFYYIAIHSITV 305

Query: 310 DGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPG 369
           DGV LPINPAVWA+D+ GNGGTVVDSGTTLT+LA+ AYD+VL A RR+VKLP  +ELTPG
Sbjct: 306 DGVKLPINPAVWAMDKQGNGGTVVDSGTTLTYLAKAAYDEVLKAVRRRVKLPSASELTPG 365

Query: 370 FDLCLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSV 429
           FDLC+NAS  S RPSLPRL FRL GGAV +PPPRNYFLETEERVMCLAIRPVDSGNGFSV
Sbjct: 366 FDLCVNASDSSRRPSLPRLRFRLGGGAVFAPPPRNYFLETEERVMCLAIRPVDSGNGFSV 425

Query: 430 IGNLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           IGNLMQQGFLLEFDKDA+RLGFSRRGCGLP
Sbjct: 426 IGNLMQQGFLLEFDKDAARLGFSRRGCGLP 449

BLAST of Moc04g24310 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 704.1 bits (1816), Expect = 7.7e-199
Identity = 356/446 (79.82%), Postives = 386/446 (86.55%), Query Frame = 0

Query: 19  LLLLFS-----NATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRNRNAV 78
           +LLLFS     NA AA  +LKLPLLH NPFSSPS AL+ D HRLS+LFS+  R  N   +
Sbjct: 15  ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPN-PTL 74

Query: 79  KSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFLP 138
           KSPLISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCS CRNCSHH PSSAFLP
Sbjct: 75  KSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLP 134

Query: 139 RHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTLK 198
           RHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + Y+YADGS S+GFFSK+TTTLK
Sbjct: 135 RHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLK 194

Query: 199 TLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFSY 258
           TLSG+E RL +LSFGCGFRISGPSVSG+ FNGARGVMGLGRGPISFS+QLGRRFGNKFSY
Sbjct: 195 TLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSY 254

Query: 259 CLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDGVT 318
           CLMDYTLSPPPTSYLMI G  RS  V+NA++ISYTPL INPLSPTFYYI VKSI VDGV 
Sbjct: 255 CLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVK 314

Query: 319 LPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPGFDLC 378
           LPINP VWAIDE GNGGTVVDSGTTLT+LAE AY +VL A R++VKLP  AELTPGFDLC
Sbjct: 315 LPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAAELTPGFDLC 374

Query: 379 LNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVIGNL 438
           +N S ES RPSLPR+ F+L  GAV +PP RNYFLETEE VMCL+IR V+ GNGFSVIGNL
Sbjct: 375 VNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSIRAVEGGNGFSVIGNL 434

Query: 439 MQQGFLLEFDKDASRLGFSRRGCGLP 456
           MQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 435 MQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Moc04g24310 vs. NCBI nr
Match: XP_022928946.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 703.4 bits (1814), Expect = 1.3e-198
Identity = 357/448 (79.69%), Postives = 387/448 (86.38%), Query Frame = 0

Query: 18  LLLLLFS-----NATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRNRNA 77
           L+LLLFS     NA AA  +LKLPLLH NPFSSPS AL+ D HRLS+LFS+  R  N   
Sbjct: 14  LILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPN-PT 73

Query: 78  VKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFL 137
           +KSPLISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCS CRNCSHH PSSAFL
Sbjct: 74  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 133

Query: 138 PRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTL 197
           PRHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + Y+YADGS S+GFFSK+TTTL
Sbjct: 134 PRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTL 193

Query: 198 KTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFS 257
           KTL+G+E RL +LSFGCGFRISGPSVSG+ FNGARGVMGLGRGPISFS+QLGRRFGNKFS
Sbjct: 194 KTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFS 253

Query: 258 YCLMDYTLSPPPTSYLMIGA--RRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDG 317
           YCLMDYTLSPPPTSYLMIG   RR P V+NA++ISYTPL INPLSPTFYYI VKSI VDG
Sbjct: 254 YCLMDYTLSPPPTSYLMIGGGLRRLP-VTNATKISYTPLLINPLSPTFYYIAVKSITVDG 313

Query: 318 VTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPGFD 377
           V LPINP +WAIDE GNGGTVVDSGTTLT+LAE AY +VL A R++VKLP  AELTPGFD
Sbjct: 314 VKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFD 373

Query: 378 LCLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVIG 437
           LC+N S ES RPSLPR+ F+L  GAV  PP RNYFLETEE VMCLAIR V+ GNGFSVIG
Sbjct: 374 LCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNGFSVIG 433

Query: 438 NLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           NLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 434 NLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Moc04g24310 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 703.4 bits (1814), Expect = 1.3e-198
Identity = 357/447 (79.87%), Postives = 386/447 (86.35%), Query Frame = 0

Query: 18  LLLLLFS-----NATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRNRNA 77
           L+LLLFS     NA AA  +LKLPLLH NPFSSPS AL+ D HRLS+LFS+  R  N   
Sbjct: 14  LILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPN-PT 73

Query: 78  VKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFL 137
           +KSPLISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCS CRNCSHH PSSAFL
Sbjct: 74  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 133

Query: 138 PRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTL 197
           PRHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + Y+YADGS S+GFFSK+TTTL
Sbjct: 134 PRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTL 193

Query: 198 KTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFS 257
           KTLSG+E RL +LSFGCGFRISGPSVSG+ FNGARGVMGLGRGPISFS+QLGRRFGNKFS
Sbjct: 194 KTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFS 253

Query: 258 YCLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDGV 317
           YCLMDYTLSPPPTSYLMI G  RS  V+NA++ISYTPL INPLSPTFYYI VKSI VDGV
Sbjct: 254 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGV 313

Query: 318 TLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPGFDL 377
            LPINP VWAIDE GNGGTVVDSGTTLT+LAE AY +VL A R++VKLP  AELTPGFDL
Sbjct: 314 KLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAAELTPGFDL 373

Query: 378 CLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVIGN 437
           C+N S ES RPSLPR+ FR+  GAV +PP RNYFLET E VMCLAIR V+ GNGFSVIGN
Sbjct: 374 CVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEGGNGFSVIGN 433

Query: 438 LMQQGFLLEFDKDASRLGFSRRGCGLP 456
           LMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 434 LMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Moc04g24310 vs. NCBI nr
Match: KAG7017139.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 702.2 bits (1811), Expect = 2.9e-198
Identity = 355/446 (79.60%), Postives = 386/446 (86.55%), Query Frame = 0

Query: 19  LLLLFS-----NATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRNRNAV 78
           +LLLFS     NA AA  +LKLPLLH NPFSSPS AL+ D HRLS+LFS+  R  N   +
Sbjct: 11  ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPN-PTL 70

Query: 79  KSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFLP 138
           KSPLISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCS CRNCSHH PSSAFLP
Sbjct: 71  KSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLP 130

Query: 139 RHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTLK 198
           RHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + Y+YADGS S+GFFSK+TTTLK
Sbjct: 131 RHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLK 190

Query: 199 TLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFSY 258
           TLSG+E RL +LSFGCGFRISGPSVSG+ FNGARGVMGLGRGPISFS+QLGRRFGNKFSY
Sbjct: 191 TLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSY 250

Query: 259 CLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDGVT 318
           CLMDYTLSPPPTSYLMI G  RS  V+NA++ISYTPL INPLSPTFYYI VKSI VDGV 
Sbjct: 251 CLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVK 310

Query: 319 LPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPGFDLC 378
           LPINP VWAI+E GNGGTVVDSGTTLT+LAE AY +VL A R++VKLP  AELTPGFDLC
Sbjct: 311 LPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLC 370

Query: 379 LNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVIGNL 438
           +N S ES RPSLPR+ F+L  GAV +PP RNYFL+TEE VMCLAIR V+ GNGFSVIGNL
Sbjct: 371 VNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGGNGFSVIGNL 430

Query: 439 MQQGFLLEFDKDASRLGFSRRGCGLP 456
           MQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 431 MQQGFLLEFDKEASRLGFSRRGCGLP 455

BLAST of Moc04g24310 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 2.4e-62
Identity = 154/418 (36.84%), Postives = 223/418 (53.35%), Query Frame = 0

Query: 47  SHALAFDAHRLSILFSSRNRNRNRNAVKSP--------LISGASTGSGQYFVDLRLGTPP 106
           S  L  D+ R+  + +   +   RN   +P        ++SG S GSG+YF  L +GTP 
Sbjct: 93  SSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPA 152

Query: 107 QSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFLPRHSSSFSPHHCFDPACRLVPHARLC 166
           + + +V DTGSD+VW++C+PCR C +      F PR S +++   C  P CR +  A  C
Sbjct: 153 RYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAG-C 212

Query: 167 NHTRLHSPCPYHYSYADGSLSAGFFSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSG 226
           N  R    C Y  SY DGS + G FS +T T +       R+  ++ GCG    G     
Sbjct: 213 NTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRVKGVALGCGHDNEG----- 272

Query: 227 STFNGARGVMGLGRGPISFSSQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSN 286
             F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S  P+S +   A    AVS 
Sbjct: 273 -LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNA----AVSR 332

Query: 287 ASRISYTPLQINPLSPTFYYIRVKSIAVDGVTLP-INPAVWAIDELGNGGTVVDSGTTLT 346
            +R  +TPL  NP   TFYY+ +  I+V G  +P +  +++ +D++GNGG ++DSGT++T
Sbjct: 333 IAR--FTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVT 392

Query: 347 FLAEPAYDQVLAAFRRQVKLPRPAELTPGFDLCLNASGESRPSLPR--LSFRLAGGAVLS 406
            L  PAY  +  AFR   K  + A     FD C + S  +   +P   L FR   GA +S
Sbjct: 393 RLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR---GADVS 452

Query: 407 PPPRNYFLETEER-VMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDKDASRLGFSRRGC 453
            P  NY +  +     C A     +  G S+IGN+ QQGF + +D  +SR+GF+  GC
Sbjct: 453 LPATNYLIPVDTNGKFCFAF--AGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Moc04g24310 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 4.9e-55
Identity = 164/453 (36.20%), Postives = 228/453 (50.33%), Query Frame = 0

Query: 14  LFPLLLLLLFSNATAATQFLKLPLLHSN-----PFSSPSHALAFDAHRLSILFSSRNRNR 73
           L   LLL+  +++   T  ++L L H +     P S     +  D  R S++  SR RN 
Sbjct: 31  LITTLLLITVADSMKDTS-VRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLI--SRKRN- 90

Query: 74  NRNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPS 133
           +   VK  L SG   G+ QYF ++R+GTP +   +V DTGS+L WV      NC + A  
Sbjct: 91  STVGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWV------NCRYRARG 150

Query: 134 S----AFLPRHSSSFSPHHCFDPACR--LVPHARLCNHTRLHSPCPYHYSYADGSLSAGF 193
                 F    S SF    C    C+  L+    L       +PC Y Y YADGS + G 
Sbjct: 151 KDNRRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGV 210

Query: 194 FSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLG 253
           F+K+T T+   +G  ARLP    GC       S +G +F GA GV+GL     SF+S   
Sbjct: 211 FAKETITVGLTNGRMARLPGHLIGC-----SSSFTGQSFQGADGVLGLAFSDFSFTSTAT 270

Query: 254 RRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLSPTFYYIRVK 313
             +G KFSYCL+D+  +   ++YL+ G+ RS     A R + TPL +  + P FY I V 
Sbjct: 271 SLYGAKFSYCLVDHLSNKNVSNYLIFGSSRS--TKTAFRRT-TPLDLTRI-PPFYAINVI 330

Query: 314 SIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQ-VKLPRPA 373
            I++    L I   VW  D    GGT++DSGT+LT LA+ AY QV+    R  V+L R  
Sbjct: 331 GISLGYDMLDIPSQVW--DATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRVK 390

Query: 374 ELTPGFDLCLN-ASGESRPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSG 433
                 + C +  SG +   LP+L+F L GGA   P  ++Y ++    V CL    V +G
Sbjct: 391 PEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGF--VSAG 450

Query: 434 N-GFSVIGNLMQQGFLLEFDKDASRLGFSRRGC 453
               +VIGN+MQQ +L EFD  AS L F+   C
Sbjct: 451 TPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

BLAST of Moc04g24310 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 4.6e-53
Identity = 140/392 (35.71%), Postives = 203/392 (51.79%), Query Frame = 0

Query: 66  RNRNRNAV---KSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNC 125
           R R+ NA+    S + +    G G+Y +++ +GTP  S   + DTGSDL+W +C PC  C
Sbjct: 71  RMRSINAMLQSSSGIETPVYAGDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC 130

Query: 126 SHHAPSSAFLPRHSSSFSPHHCFDPACRLVPHARLCNHTRLHSPCPYHYSYADGSLSAGF 185
               P+  F P+ SSSFS   C    C+ +P +  CN    ++ C Y Y Y DGS + G+
Sbjct: 131 -FSQPTPIFNPQDSSSFSTLPCESQYCQDLP-SETCN----NNECQYTYGYGDGSTTQGY 190

Query: 186 FSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLG 245
            + +T T +T S     +PN++FGCG    G        NGA G++G+G GP+S  SQLG
Sbjct: 191 MATETFTFETSS-----VPNIAFGCGEDNQGFGQG----NGA-GLIGMGWGPLSLPSQLG 250

Query: 246 RRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLSPTFYYIRVK 305
                +FSYC+  Y  S P T  L   A   P  S +     T L  + L+PT+YYI ++
Sbjct: 251 ---VGQFSYCMTSYGSSSPSTLALGSAASGVPEGSPS-----TTLIHSSLNPTYYYITLQ 310

Query: 306 SIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAE 365
            I V G  L I  + + + + G GG ++DSGTTLT+L + AY+ V  AF  Q+ LP   E
Sbjct: 311 GITVGGDNLGIPSSTFQLQDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDE 370

Query: 366 LTPGFDLCL-NASGESRPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGN 425
            + G   C    S  S   +P +S +  GG VL+   +N  +   E V+CLA+    S  
Sbjct: 371 SSSGLSTCFQQPSDGSTVQVPEISMQFDGG-VLNLGEQNILISPAEGVICLAMGS-SSQL 430

Query: 426 GFSVIGNLMQQGFLLEFDKDASRLGFSRRGCG 454
           G S+ GN+ QQ   + +D     + F    CG
Sbjct: 431 GISIFGNIQQQETQVLYDLQNLAVSFVPTQCG 436

BLAST of Moc04g24310 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 209.5 bits (532), Expect = 7.8e-53
Identity = 132/384 (34.38%), Postives = 196/384 (51.04%), Query Frame = 0

Query: 71  NAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSA 130
           N   S ++SG   GSG+YFV + +G+PP+   +V D+GSD+VWV+C PC+ C +      
Sbjct: 114 NDFGSDIVSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPV 173

Query: 131 FLPRHSSSFSPHHCFDPACRLVPHARLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTL 190
           F P  S S++   C    C      R+ N       C Y   Y DGS     ++K T  L
Sbjct: 174 FDPAKSGSYTGVSCGSSVC-----DRIENSGCHSGGCRYEVMYGDGS-----YTKGTLAL 233

Query: 191 KTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFS 250
           +TL+ A+  + N++ GCG R  G       F GA G++G+G G +SF  QL  + G  F 
Sbjct: 234 ETLTFAKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFG 293

Query: 251 YCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDGVT 310
           YCL+  +     T  L+ G    P  +     S+ PL  NP +P+FYY+ +K + V GV 
Sbjct: 294 YCLV--SRGTDSTGSLVFGREALPVGA-----SWVPLVRNPRAPSFYYVGLKGLGVGGVR 353

Query: 311 LPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQ-VKLPRPAELTPGFDL 370
           +P+   V+ + E G+GG V+D+GT +T L   AY      F+ Q   LPR + ++  FD 
Sbjct: 354 IPLPDGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDT 413

Query: 371 CLNASGESRPSLPRLSFRLAGGAVLSPPPRNYFLETEER-VMCLAIRPVDSGNGFSVIGN 430
           C + SG     +P +SF    G VL+ P RN+ +  ++    C A     S  G S+IGN
Sbjct: 414 CYDLSGFVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAF--AASPTGLSIIGN 470

Query: 431 LMQQGFLLEFDKDASRLGFSRRGC 453
           + Q+G  + FD     +GF    C
Sbjct: 474 IQQEGIQVSFDGANGFVGFGPNVC 470

BLAST of Moc04g24310 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-52
Identity = 140/397 (35.26%), Postives = 199/397 (50.13%), Query Frame = 0

Query: 63  SRNRNRNRNAVKSPLISGAST----GSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSP 122
           SR   R    +  P  SG  T    G G+Y ++L +GTP Q    + DTGSDL+W +C P
Sbjct: 68  SRRLQRLEAMLNGP--SGVETSVYAGDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQP 127

Query: 123 CRNCSHHAPSSAFLPRHSSSFSPHHCFDPACRLVPHARLCNHTRLHSPCPYHYSYADGSL 182
           C  C + + +  F P+ SSSFS   C    C+      L + T  ++ C Y Y Y DGS 
Sbjct: 128 CTQCFNQS-TPIFNPQGSSSFSTLPCSSQLCQ-----ALSSPTCSNNFCQYTYGYGDGSE 187

Query: 183 SAGFFSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFS 242
           + G    +T T  ++S     +PN++FGCG    G        NGA G++G+GRGP+S  
Sbjct: 188 TQGSMGTETLTFGSVS-----IPNITFGCGENNQGFGQG----NGA-GLVGMGRGPLSLP 247

Query: 243 SQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLSPTFYY 302
           SQL      KFSYC+     S P  S L++G+  +   + +     T L  +   PTFYY
Sbjct: 248 SQLD---VTKFSYCMTPIGSSTP--SNLLLGSLANSVTAGSPN---TTLIQSSQIPTFYY 307

Query: 303 IRVKSIAVDGVTLPINPAVWAID-ELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKL 362
           I +  ++V    LPI+P+ +A++   G GG ++DSGTTLT+    AY  V   F  Q+ L
Sbjct: 308 ITLNGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINL 367

Query: 363 PRPAELTPGFDLCLNA-SGESRPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRP 422
           P     + GFDLC    S  S   +P       GG  L  P  NYF+     ++CLA+  
Sbjct: 368 PVVNGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGD-LELPSENYFISPSNGLICLAMG- 427

Query: 423 VDSGNGFSVIGNLMQQGFLLEFDKDASRLGFSRRGCG 454
             S  G S+ GN+ QQ  L+ +D   S + F+   CG
Sbjct: 428 -SSSQGMSIFGNIQQQNMLVVYDTGNSVVSFASAQCG 435

BLAST of Moc04g24310 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 6.3e-199
Identity = 357/447 (79.87%), Postives = 386/447 (86.35%), Query Frame = 0

Query: 18  LLLLLFS-----NATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRNRNA 77
           L+LLLFS     NA AA  +LKLPLLH NPFSSPS AL+ D HRLS+LFS+  R  N   
Sbjct: 14  LILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPN-PT 73

Query: 78  VKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFL 137
           +KSPLISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCS CRNCSHH PSSAFL
Sbjct: 74  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 133

Query: 138 PRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTL 197
           PRHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + Y+YADGS S+GFFSK+TTTL
Sbjct: 134 PRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTL 193

Query: 198 KTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFS 257
           KTLSG+E RL +LSFGCGFRISGPSVSG+ FNGARGVMGLGRGPISFS+QLGRRFGNKFS
Sbjct: 194 KTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFS 253

Query: 258 YCLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDGV 317
           YCLMDYTLSPPPTSYLMI G  RS  V+NA++ISYTPL INPLSPTFYYI VKSI VDGV
Sbjct: 254 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGV 313

Query: 318 TLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPGFDL 377
            LPINP VWAIDE GNGGTVVDSGTTLT+LAE AY +VL A R++VKLP  AELTPGFDL
Sbjct: 314 KLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKLPAAAELTPGFDL 373

Query: 378 CLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVIGN 437
           C+N S ES RPSLPR+ FR+  GAV +PP RNYFLET E VMCLAIR V+ GNGFSVIGN
Sbjct: 374 CVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRAVEGGNGFSVIGN 433

Query: 438 LMQQGFLLEFDKDASRLGFSRRGCGLP 456
           LMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 434 LMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Moc04g24310 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 703.4 bits (1814), Expect = 6.3e-199
Identity = 357/448 (79.69%), Postives = 387/448 (86.38%), Query Frame = 0

Query: 18  LLLLLFS-----NATAATQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRNRNRNA 77
           L+LLLFS     NA AA  +LKLPLLH NPFSSPS AL+ D HRLS+LFS+  R  N   
Sbjct: 14  LILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPN-PT 73

Query: 78  VKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFL 137
           +KSPLISGASTGSGQYFVDLR+GTPPQSLLLVADTGSDLVWVKCS CRNCSHH PSSAFL
Sbjct: 74  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 133

Query: 138 PRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTL 197
           PRHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + Y+YADGS S+GFFSK+TTTL
Sbjct: 134 PRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTL 193

Query: 198 KTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKFS 257
           KTL+G+E RL +LSFGCGFRISGPSVSG+ FNGARGVMGLGRGPISFS+QLGRRFGNKFS
Sbjct: 194 KTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFS 253

Query: 258 YCLMDYTLSPPPTSYLMIGA--RRSPAVSNASRISYTPLQINPLSPTFYYIRVKSIAVDG 317
           YCLMDYTLSPPPTSYLMIG   RR P V+NA++ISYTPL INPLSPTFYYI VKSI VDG
Sbjct: 254 YCLMDYTLSPPPTSYLMIGGGLRRLP-VTNATKISYTPLLINPLSPTFYYIAVKSITVDG 313

Query: 318 VTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELTPGFD 377
           V LPINP +WAIDE GNGGTVVDSGTTLT+LAE AY +VL A R++VKLP  AELTPGFD
Sbjct: 314 VKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFD 373

Query: 378 LCLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVIG 437
           LC+N S ES RPSLPR+ F+L  GAV  PP RNYFLETEE VMCLAIR V+ GNGFSVIG
Sbjct: 374 LCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRAVEGGNGFSVIG 433

Query: 438 NLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           NLMQQGFLLEFDK+ASRLGFSRRGCGLP
Sbjct: 434 NLMQQGFLLEFDKEASRLGFSRRGCGLP 459

BLAST of Moc04g24310 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 2.3e-196
Identity = 354/454 (77.97%), Postives = 385/454 (84.80%), Query Frame = 0

Query: 18  LLLLLFS---------NATAA---TQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRN 77
           LL+LLFS         NATA      FLKLPLLH  PFSSPS +L+ D HRLS+LFS   
Sbjct: 11  LLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLSLLFS--- 70

Query: 78  RNRNRNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHH 137
             R    +KSPLISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKCS CRNCSHH
Sbjct: 71  --RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSACRNCSHH 130

Query: 138 APSSAFLPRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYADGSLSAGFF 197
            PSSAFLPRHSSSFSP HCFDP CRL+PHA   LCNHTRLHSPC + YSYADGSLS+GFF
Sbjct: 131 PPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGSLSSGFF 190

Query: 198 SKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGR 257
           SK+TTTLK+LSG+E  L  LSFGCGFRISGPSVSG+ FNGARGVMGLGRG ISFSSQLGR
Sbjct: 191 SKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISFSSQLGR 250

Query: 258 RFGNKFSYCLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVK 317
           RFGNKFSYCLMDYTLSPPPTS+LMI G   S  ++NA++ISYTPLQINPLSPTFYYI + 
Sbjct: 251 RFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTFYYITIH 310

Query: 318 SIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAE 377
           SI +DGV LPINPAVW IDE GNGGTVVDSGTTLT+L + AY++VL + RR+VKLP  AE
Sbjct: 311 SITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVKLPNAAE 370

Query: 378 LTPGFDLCLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGN 437
           LTPGFDLC+NASGES RPSLPRL FRL GGAV +PPPRNYFLETEE VMCLAIR V+SGN
Sbjct: 371 LTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIRAVESGN 430

Query: 438 GFSVIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           GFSVIGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 431 GFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Moc04g24310 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 3.3e-195
Identity = 357/463 (77.11%), Postives = 385/463 (83.15%), Query Frame = 0

Query: 5   PTLSF-PFPQLFPLLLLLLF----SNAT---AATQFLKLPLLHSNPFSSPSHALAFDAHR 64
           P LS  PF  L PL    L      NAT   AA  FLKLPLLH  PFSSPS +L+ D HR
Sbjct: 2   PMLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHR 61

Query: 65  LSILFSSRNRNRNRNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKC 124
           LS+LFS     R    +KSPLISGASTGSGQYFVD+RLGTPPQSLLLVADTGSDLVWVKC
Sbjct: 62  LSLLFS-----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKC 121

Query: 125 SPCRNCSHHAPSSAFLPRHSSSFSPHHCFDPACRLVPHA--RLCNHTRLHSPCPYHYSYA 184
           S CRNCSHH PSSAF PRHSSSFSP HCFDP CRL+PHA    CNHT LHSPC + YSYA
Sbjct: 122 SACRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYA 181

Query: 185 DGSLSAGFFSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGP 244
           DGSLS+GFFSK+TTTLKTLSG+E  L  LSFGCGFRISGPSVSG+ FNGARGVMGLGRG 
Sbjct: 182 DGSLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGS 241

Query: 245 ISFSSQLGRRFGNKFSYCLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLS 304
           ISFSSQLGRRFGNKFSYCLMDYTLSPPPTS+LMI G   S  V+NA++ISYTPLQINPLS
Sbjct: 242 ISFSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLS 301

Query: 305 PTFYYIRVKSIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRR 364
           PTFYYI + SI +DGV LPINPAVW IDE GNGGTVVDSGTTLT+L + AY++VL + RR
Sbjct: 302 PTFYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRR 361

Query: 365 QVKLPRPAELTPGFDLCLNASGES-RPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCL 424
           +VKLP  AELTPGFDLC+NASGES RPSLPRL FRL GGAV +PPPRNYFLETEE VMCL
Sbjct: 362 RVKLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCL 421

Query: 425 AIRPVDSGNGFSVIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           AIR V+SGNGFSVIGNLMQQGFLLEFDK+ SRLGF+RRGCGLP
Sbjct: 422 AIRAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

BLAST of Moc04g24310 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 679.5 bits (1752), Expect = 9.8e-192
Identity = 346/454 (76.21%), Postives = 381/454 (83.92%), Query Frame = 0

Query: 12  PQLFPLLLLLLFSNATAA----TQFLKLPLLHSNPFSSPSHALAFDAHRLSILFSSRNRN 71
           PQ   LLLLLL S A  +    +Q+LK PLLH+NPFSSPS AL+ D HRLS+LFS+   +
Sbjct: 5   PQFLLLLLLLLSSLADLSNAIPSQYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSA---H 64

Query: 72  RNRNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAP 131
           R+   +KSPLISGASTGSGQYFV+L LGTPPQSLLLV DTGSDLVWVKCSPCRNCSHH P
Sbjct: 65  RHSPTLKSPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCSHHPP 124

Query: 132 SSAFLPRHSSSFSPHHCFDPACRLVPH--ARLCNHTRLHSPCPYHYSYADGSLSAGFFSK 191
           SSAF PRHSSSFSP HCFDP CRL+PH  +  CNHT LHSPC + YSYAD SLS+GFFSK
Sbjct: 125 SSAFFPRHSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSGFFSK 184

Query: 192 DTTTLKTLSG--AEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGR 251
           D TT  T SG   + RL +LSFGCGFRISGPSVSG+ F GARGVMGLGRGPISFSSQLG 
Sbjct: 185 DVTTFNTFSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSSQLGH 244

Query: 252 RFGNKFSYCLMDYTLSPPPTSYLMI-GARRSPAVSNASRISYTPLQINPLSPTFYYIRVK 311
           RFGN FSYCLMDYTLSPPPTSYLMI G  RS  V+NAS+ISYTPLQINPLSPTFYYI VK
Sbjct: 245 RFGNTFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVK 304

Query: 312 SIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAE 371
           SI VDGV LPINP VWAIDE GNGGTVVDSGTTLT+LAE AY++VL A RR+VKLPR  +
Sbjct: 305 SITVDGVKLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQ 364

Query: 372 LTPGFDLCLNASGESR-PSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGN 431
           L+PGFDLC+NAS ESR  SLP++ FR+ GG V +PP RNYF+ETEE VMCLAIRPVDSGN
Sbjct: 365 LSPGFDLCVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGN 424

Query: 432 GFSVIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           GFSVIGNLMQQGFLLEFD++ SR+GFSRRGCGLP
Sbjct: 425 GFSVIGNLMQQGFLLEFDREKSRMGFSRRGCGLP 455

BLAST of Moc04g24310 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 597.4 bits (1539), Expect = 9.4e-171
Identity = 301/458 (65.72%), Postives = 357/458 (77.95%), Query Frame = 0

Query: 11  FPQLFPL-----LLLLLFSNATAAT---QFLKLPLLHSNPFSSPSHALAFDAHRLSILFS 70
           FP +F L     L LL  SN  A +   ++LKLPLL  +PF SP+ ALA D  RL  L  
Sbjct: 2   FPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFL-- 61

Query: 71  SRNRNRNRNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNC 130
              R +    VKSP++SGA++GSGQYFVDLR+G PPQSLLL+ADTGSDLVWVKCS CRNC
Sbjct: 62  -SLRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNC 121

Query: 131 SHHAPSSAFLPRHSSSFSPHHCFDPACRLVP---HARLCNHTRLHSPCPYHYSYADGSLS 190
           SHH+P++ F PRHSS+FSP HC+DP CRLVP    A +CNHTR+HS C Y Y YADGSL+
Sbjct: 122 SHHSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLT 181

Query: 191 AGFFSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSS 250
           +G F+++TT+LKT SG EARL +++FGCGFRISG SVSG++FNGA GVMGLGRGPISF+S
Sbjct: 182 SGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFAS 241

Query: 251 QLGRRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLSPTFYYI 310
           QLGRRFGNKFSYCLMDYTLSPPPTSYL+IG          S++ +TPL  NPLSPTFYY+
Sbjct: 242 QLGRRFGNKFSYCLMDYTLSPPPTSYLIIG----NGGDGISKLFFTPLLTNPLSPTFYYV 301

Query: 311 RVKSIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPR 370
           ++KS+ V+G  L I+P++W ID+ GNGGTVVDSGTTL FLAEPAY  V+AA RR+VKLP 
Sbjct: 302 KLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPI 361

Query: 371 PAELTPGFDLCLNASGESRPS--LPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPV 430
              LTPGFDLC+N SG ++P   LPRL F  +GGAV  PPPRNYF+ETEE++ CLAI+ V
Sbjct: 362 ADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSV 421

Query: 431 DSGNGFSVIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           D   GFSVIGNLMQQGFL EFD+D SRLGFSRRGC LP
Sbjct: 422 DPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

BLAST of Moc04g24310 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 409.1 bits (1050), Expect = 4.8e-114
Identity = 231/458 (50.44%), Postives = 271/458 (59.17%), Query Frame = 0

Query: 11  FPQLFPL-----LLLLLFSNATAAT---QFLKLPLLHSNPFSSPSHALAFDAHRLSILFS 70
           FP +F L     L LL  SN  A +   ++LKLPLL  +PF SP+ ALA D  RL  L  
Sbjct: 2   FPLIFFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLHFL-- 61

Query: 71  SRNRNRNRNAVKSPLISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNC 130
              R +    VKSP++SGA++GSGQYFVDLR+G PPQSLLL+ADTGSDLVWVKCS CRNC
Sbjct: 62  -SLRRKPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNC 121

Query: 131 SHHAPSSAFLPRHSSSFSPHHCFDPACRLVP---HARLCNHTRLHSPCPYHYSYADGSLS 190
           SHH+P++ F PRHSS+FSP HC+DP CRLVP    A +CNHTR+HS C Y Y YADGSL+
Sbjct: 122 SHHSPATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSLT 181

Query: 191 AGFFSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSS 250
           +G F+++TT+LKT SG EARL +++FGCGFRISG SVS                      
Sbjct: 182 SGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS---------------------- 241

Query: 251 QLGRRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLSPTFYYI 310
                                                                       
Sbjct: 242 ------------------------------------------------------------ 301

Query: 311 RVKSIAVDGVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPR 370
                                   GNGGTVVDSGTTL FLAEPAY  V+AA RR+VKLP 
Sbjct: 302 ------------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPI 350

Query: 371 PAELTPGFDLCLNASGESRPS--LPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPV 430
              LTPGFDLC+N SG ++P   LPRL F  +GGAV  PPPRNYF+ETEE++ CLAI+ V
Sbjct: 362 ADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSV 350

Query: 431 DSGNGFSVIGNLMQQGFLLEFDKDASRLGFSRRGCGLP 456
           D   GFSVIGNLMQQGFL EFD+D SRLGFSRRGC LP
Sbjct: 422 DPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

BLAST of Moc04g24310 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 243.4 bits (620), Expect = 3.5e-64
Identity = 152/388 (39.18%), Postives = 211/388 (54.38%), Query Frame = 0

Query: 77  LISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFLPRHS 136
           L SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C PC +C  H     + P+ S
Sbjct: 149 LESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDC-FHQNGMFYDPKTS 208

Query: 137 SSFSPHHCFDPACRLVPHAR---LCNHTRLHSPCPYHYSYADGSLSAGFFSKDTTT--LK 196
           +SF    C DP C L+        C     +  CPY Y Y D S + G F+ +T T  L 
Sbjct: 209 ASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETFTVNLT 268

Query: 197 TLSG--AEARLPNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNKF 256
           T  G  +E ++ N+ FGCG    G       F+GA G++GLGRGP+SFSSQL   +G+ F
Sbjct: 269 TTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLYGHSF 328

Query: 257 SYCLMDYTLSPPPTSYLMIGARRSPAVSNASRISYTPLQINPLS--PTFYYIRVKSIAVD 316
           SYCL+D   +   +S L+ G  +   + N + +++T       +   TFYYI++KSI V 
Sbjct: 329 SYCLVDRNSNTNVSSKLIFGEDKD--LLNHTNLNFTSFVNGKENSVETFYYIQIKSILVG 388

Query: 317 GVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPA-ELTPG 376
           G  L I    W I   G+GGT++DSGTTL++ AEPAY+ +   F  ++K   P     P 
Sbjct: 389 GKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRDFPV 448

Query: 377 FDLCLNASG--ESRPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFS 436
            D C N SG  E+   LP L      G V + P  N F+   E ++CLAI        FS
Sbjct: 449 LDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKST-FS 508

Query: 437 VIGNLMQQGFLLEFDKDASRLGFSRRGC 453
           +IGN  QQ F + +D   SRLGF+   C
Sbjct: 509 IIGNYQQQNFHILYDTKRSRLGFTPTKC 524

BLAST of Moc04g24310 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.1 bits (614), Expect = 1.7e-63
Identity = 154/418 (36.84%), Postives = 223/418 (53.35%), Query Frame = 0

Query: 47  SHALAFDAHRLSILFSSRNRNRNRNAVKSP--------LISGASTGSGQYFVDLRLGTPP 106
           S  L  D+ R+  + +   +   RN   +P        ++SG S GSG+YF  L +GTP 
Sbjct: 93  SSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPA 152

Query: 107 QSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFLPRHSSSFSPHHCFDPACRLVPHARLC 166
           + + +V DTGSD+VW++C+PCR C +      F PR S +++   C  P CR +  A  C
Sbjct: 153 RYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPRKSKTYATIPCSSPHCRRLDSAG-C 212

Query: 167 NHTRLHSPCPYHYSYADGSLSAGFFSKDTTTLKTLSGAEARLPNLSFGCGFRISGPSVSG 226
           N  R    C Y  SY DGS + G FS +T T +       R+  ++ GCG    G     
Sbjct: 213 NTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRVKGVALGCGHDNEG----- 272

Query: 227 STFNGARGVMGLGRGPISFSSQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGARRSPAVSN 286
             F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S  P+S +   A    AVS 
Sbjct: 273 -LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASSKPSSVVFGNA----AVSR 332

Query: 287 ASRISYTPLQINPLSPTFYYIRVKSIAVDGVTLP-INPAVWAIDELGNGGTVVDSGTTLT 346
            +R  +TPL  NP   TFYY+ +  I+V G  +P +  +++ +D++GNGG ++DSGT++T
Sbjct: 333 IAR--FTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTSVT 392

Query: 347 FLAEPAYDQVLAAFRRQVKLPRPAELTPGFDLCLNASGESRPSLPR--LSFRLAGGAVLS 406
            L  PAY  +  AFR   K  + A     FD C + S  +   +P   L FR   GA +S
Sbjct: 393 RLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFR---GADVS 452

Query: 407 PPPRNYFLETEER-VMCLAIRPVDSGNGFSVIGNLMQQGFLLEFDKDASRLGFSRRGC 453
            P  NY +  +     C A     +  G S+IGN+ QQGF + +D  +SR+GF+  GC
Sbjct: 453 LPATNYLIPVDTNGKFCFAF--AGTMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGC 484

BLAST of Moc04g24310 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 236.5 bits (602), Expect = 4.2e-62
Identity = 150/386 (38.86%), Postives = 210/386 (54.40%), Query Frame = 0

Query: 77  LISGASTGSGQYFVDLRLGTPPQSLLLVADTGSDLVWVKCSPCRNCSHHAPSSAFL-PRH 136
           L SG + GSG+YF+D+ +G+PP+   L+ DTGSDL W++C PC +C     + AF  P+ 
Sbjct: 159 LESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQ--NGAFYDPKA 218

Query: 137 SSSFSPHHCFDPACRLV--PHARL-CNHTRLHSPCPYHYSYADGSLSAGFFSKDTTTLK- 196
           S+S+    C D  C LV  P   + C     +  CPY+Y Y D S + G F+ +T T+  
Sbjct: 219 SASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETFTVNL 278

Query: 197 TLSGAEARL---PNLSFGCGFRISGPSVSGSTFNGARGVMGLGRGPISFSSQLGRRFGNK 256
           T +G  + L    N+ FGCG    G       F+GA G++GLGRGP+SFSSQL   +G+ 
Sbjct: 279 TTNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSLYGHS 338

Query: 257 FSYCLMDYTLSPPPTSYLMIGARRS-PAVSNASRISYTPLQINPLSPTFYYIRVKSIAVD 316
           FSYCL+D       +S L+ G  +   +  N +  S+   + N L  TFYY+++KSI V 
Sbjct: 339 FSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKSILVA 398

Query: 317 GVTLPINPAVWAIDELGNGGTVVDSGTTLTFLAEPAYDQVLAAFRRQVKLPRPAELT-PG 376
           G  L I    W I   G GGT++DSGTTL++ AEPAY+ +      + K   P     P 
Sbjct: 399 GEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYRDFPI 458

Query: 377 FDLCLNASGESRPSLPRLSFRLAGGAVLSPPPRNYFLETEERVMCLAIRPVDSGNGFSVI 436
            D C N SG     LP L    A GAV + P  N F+   E ++CLA+      + FS+I
Sbjct: 459 LDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTPK-SAFSII 518

Query: 437 GNLMQQGFLLEFDKDASRLGFSRRGC 453
           GN  QQ F + +D   SRLG++   C
Sbjct: 519 GNYQQQNFHILYDTKRSRLGYAPTKC 532