Moc04g10630 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g10630
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr4: 7931098 .. 7931628 (-)
RNA-Seq ExpressionMoc04g10630
SyntenyMoc04g10630
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGGCAACTATTTCAAATTCTTGTGGTAAAGAGAAATTGAAATTCGCAGATGTCAGAGATGTAGCTCTTGGAGAGGAGATTCGCATAAAGAATTCTGGTATTGCGTCTACTTCTGGTATAGATTTGAATGTAGATAGAGAAACAAATAATAACAGAGACCACAGAAAATATGGAAAGTTAAGAAATAACAGAAGTAAGTCTAAAAACATCAGACTAAAATGTTAGAATTATAGTAAGACAGGACATCTGAGGAGGAACTACAAAGCCCTAAATAAAGCTGAAGGGAAAGAAGTTGGTGCAAATTTTGTTGCTCAATAAATACATGATGCTCTAGTTCTTGCAGTTGAAGGCGCTCATAACACATGGGTGGTGGATTCAGGTGCGTCTTTTCACACTACAGGACAACGTGATATTCTTGAAAATTATGTTGCAGGAAATCATGGAAAGATGTATCTTGCTGATGGAAAGCCTTTAGACATCATTGGGATTTGTGACGTTAATTTAAAAATGGCGAACAGTTCAGTCTAG

mRNA sequence

ATGAAGGCAACTATTTCAAATTCTTGTGGTAAAGAGAAATTGAAATTCGCAGATGTCAGAGATGTAGCTCTTGGAGAGGAGATTCGCATAAAGAATTCTGGTATTGCGTCTACTTCTGGTATAGATTTGAATGTAGATAGAGAAACAAATAATAACAGAGACCACAGAAAATATGGAAAGTTAAGAAATAACAGAATTGAAGGCGCTCATAACACATGGGTGGTGGATTCAGGTGCGTCTTTTCACACTACAGGACAACGTGATATTCTTGAAAATTATGTTGCAGGAAATCATGGAAAGATGTATCTTGCTGATGGAAAGCCTTTAGACATCATTGGGATTTGTGACGTTAATTTAAAAATGGCGAACAGTTCAGTCTAG

Coding sequence (CDS)

ATGAAGGCAACTATTTCAAATTCTTGTGGTAAAGAGAAATTGAAATTCGCAGATGTCAGAGATGTAGCTCTTGGAGAGGAGATTCGCATAAAGAATTCTGGTATTGCGTCTACTTCTGGTATAGATTTGAATGTAGATAGAGAAACAAATAATAACAGAGACCACAGAAAATATGGAAAGTTAAGAAATAACAGAATTGAAGGCGCTCATAACACATGGGTGGTGGATTCAGGTGCGTCTTTTCACACTACAGGACAACGTGATATTCTTGAAAATTATGTTGCAGGAAATCATGGAAAGATGTATCTTGCTGATGGAAAGCCTTTAGACATCATTGGGATTTGTGACGTTAATTTAAAAATGGCGAACAGTTCAGTCTAG

Protein sequence

MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVDRETNNNRDHRKYGKLRNNRIEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLKMANSSV
Homology
BLAST of Moc04g10630 vs. NCBI nr
Match: XP_022152845.1 (uncharacterized protein LOC111020469 [Momordica charantia])

HSP 1 Score: 122.1 bits (305), Expect = 3.5e-24
Identity = 79/175 (45.14%), Postives = 89/175 (50.86%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVDRETNNNRDHRKYGK 60
           M+A ISNSC KEKLKF DVRD AL EEIR K+SGIA TSG  LNVDR  NNNR +   GK
Sbjct: 708 MRAAISNSCEKEKLKFEDVRDAALAEEIRRKDSGIAPTSGSVLNVDRGRNNNRGYGNRGK 767

Query: 61  LRNNR-------------------------------------------------IEGAHN 120
            +NNR                                                 +E AH+
Sbjct: 768 SKNNRSRSRNSRFECWNCGKIGHLKSNCKAPKKNEGNEADANVAEQIHDALVIAVESAHD 827

Query: 121 TWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLKMANSSV 127
           TWV+DS                  GNHGK+YLADG+PLDIIGI +VNLKMAN SV
Sbjct: 828 TWVMDS------------------GNHGKVYLADGEPLDIIGIGEVNLKMANGSV 864

BLAST of Moc04g10630 vs. NCBI nr
Match: KAG6525408.1 (hypothetical protein ZIOFF_015364 [Zingiber officinale])

HSP 1 Score: 120.2 bits (300), Expect = 1.3e-23
Identity = 65/128 (50.78%), Postives = 81/128 (63.28%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSG--IASTSGIDLNVDRETNNNRDHRKY 60
           M+A +SNS G  KL F DVRD  L EE+RI +SG  +AS S +++ VD            
Sbjct: 48  MQAVVSNSVGNTKLNFNDVRDRILAEEVRIIDSGEMLASNSALNIEVDSPI--------- 107

Query: 61  GKLRNNRIEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVN 120
                       + WV+DSGASFHTT QR+ILENYVAGNHGK+YLADG+PLDI+G   + 
Sbjct: 108 ------------DPWVLDSGASFHTTAQREILENYVAGNHGKVYLADGEPLDIVGTGSIR 154

Query: 121 LKMANSSV 127
           LKM+N  V
Sbjct: 168 LKMSNGVV 154

BLAST of Moc04g10630 vs. NCBI nr
Match: RVX07359.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera])

HSP 1 Score: 117.5 bits (293), Expect = 8.5e-23
Identity = 59/129 (45.74%), Postives = 87/129 (67.44%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVD---RETNNNRDHRK 60
           MK  +SNS GKEKLK+ D+R + L EEIR++++   S SG  LN++   R+  N  +   
Sbjct: 113 MKMAVSNSTGKEKLKYNDIRYLILAEEIRLRDASETSGSGSALNLETRSRDQANKYNAGT 172

Query: 61  YGKLRNNRIEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDV 120
            GK    +++   + W++DSGASFHTT  R+I++NYVAG+ GK+YLADG  LD++G+ DV
Sbjct: 173 VGKQVTLKVDSPLDDWILDSGASFHTTPHREIIQNYVAGDFGKVYLADGSALDVVGLGDV 232

Query: 121 NLKMANSSV 127
            + + N SV
Sbjct: 233 RISLPNGSV 241

BLAST of Moc04g10630 vs. NCBI nr
Match: KAB5561215.1 (hypothetical protein DKX38_006172 [Salix brachista])

HSP 1 Score: 117.5 bits (293), Expect = 8.5e-23
Identity = 66/141 (46.81%), Postives = 91/141 (64.54%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVDRETNNNRDHRKY-- 60
           M+ TISNS GK KL + D+RD+ LGEE+R +++G +STSG  LNV+   +   +  K   
Sbjct: 95  MRTTISNSAGKSKLNYDDIRDLILGEEVRRRDAGESSTSGSTLNVETRGHIQANCYKMKK 154

Query: 61  ---GKLRNNRIEGAH-----------NTWVVDSGASFHTTGQRDILENYVAGNHGKMYLA 120
               K  N   E  H           ++WV+DSGASFHTT  ++IL+NYVAG++G +YLA
Sbjct: 155 EDDDKSANTVAEEGHEALLLSVSSPLDSWVLDSGASFHTTPHQNILQNYVAGDYGVVYLA 214

Query: 121 DGKPLDIIGICDVNLKMANSS 126
           DG+PL IIGI DV +K+ N+S
Sbjct: 215 DGEPLRIIGIGDVEIKLPNNS 235

BLAST of Moc04g10630 vs. NCBI nr
Match: PON60333.1 (Zinc finger, CCHC-type [Parasponia andersonii])

HSP 1 Score: 116.3 bits (290), Expect = 1.9e-22
Identity = 74/181 (40.88%), Postives = 97/181 (53.59%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNV---DR--ETNNNRDH 60
           M+A +SNS GKEKL+F DVRD  L EE+R  +SG  ++S   LN+   DR  E N+NR  
Sbjct: 159 MRAAVSNSVGKEKLQFNDVRDRILAEEVRRIDSGEGASSSSALNLKNRDRSSEKNSNRGR 218

Query: 61  RKYGKLRNNR-------------------------------------------------- 120
            K  K RN R                                                  
Sbjct: 219 DK-SKSRNGRNKSRSKRRMECWNCGKTGHIKKNCRAPRKDDSRNDANAITDEVQDALLLS 278

Query: 121 IEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLKMANSS 127
           ++   ++WV+DSGASFHTT  RD+LENY+AGN+GK+YLADG+PLDI+G+ D+ LKM+N S
Sbjct: 279 VDNPIDSWVLDSGASFHTTAHRDVLENYIAGNYGKVYLADGEPLDIVGMGDIRLKMSNGS 338

BLAST of Moc04g10630 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 45.1 bits (105), Expect = 7.0e-04
Identity = 21/55 (38.18%), Postives = 31/55 (56.36%), Query Frame = 0

Query: 66  IEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLK 121
           + G  + WVVD+ AS H T  RD+   YVAG+ G + + +     I GI D+ +K
Sbjct: 287 LSGPESEWVVDTAASHHATPVRDLFCRYVAGDFGTVKMGNTSYSKIAGIGDICIK 341

BLAST of Moc04g10630 vs. ExPASy TrEMBL
Match: A0A6J1DF43 (uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020469 PE=4 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.7e-24
Identity = 79/175 (45.14%), Postives = 89/175 (50.86%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVDRETNNNRDHRKYGK 60
           M+A ISNSC KEKLKF DVRD AL EEIR K+SGIA TSG  LNVDR  NNNR +   GK
Sbjct: 708 MRAAISNSCEKEKLKFEDVRDAALAEEIRRKDSGIAPTSGSVLNVDRGRNNNRGYGNRGK 767

Query: 61  LRNNR-------------------------------------------------IEGAHN 120
            +NNR                                                 +E AH+
Sbjct: 768 SKNNRSRSRNSRFECWNCGKIGHLKSNCKAPKKNEGNEADANVAEQIHDALVIAVESAHD 827

Query: 121 TWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLKMANSSV 127
           TWV+DS                  GNHGK+YLADG+PLDIIGI +VNLKMAN SV
Sbjct: 828 TWVMDS------------------GNHGKVYLADGEPLDIIGIGEVNLKMANGSV 864

BLAST of Moc04g10630 vs. ExPASy TrEMBL
Match: A0A438JEH9 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX=29760 GN=POLX_1544 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.1e-23
Identity = 59/129 (45.74%), Postives = 87/129 (67.44%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVD---RETNNNRDHRK 60
           MK  +SNS GKEKLK+ D+R + L EEIR++++   S SG  LN++   R+  N  +   
Sbjct: 113 MKMAVSNSTGKEKLKYNDIRYLILAEEIRLRDASETSGSGSALNLETRSRDQANKYNAGT 172

Query: 61  YGKLRNNRIEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDV 120
            GK    +++   + W++DSGASFHTT  R+I++NYVAG+ GK+YLADG  LD++G+ DV
Sbjct: 173 VGKQVTLKVDSPLDDWILDSGASFHTTPHREIIQNYVAGDFGKVYLADGSALDVVGLGDV 232

Query: 121 NLKMANSSV 127
            + + N SV
Sbjct: 233 RISLPNGSV 241

BLAST of Moc04g10630 vs. ExPASy TrEMBL
Match: A0A5N5N166 (Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_006172 PE=4 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.1e-23
Identity = 66/141 (46.81%), Postives = 91/141 (64.54%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVDRETNNNRDHRKY-- 60
           M+ TISNS GK KL + D+RD+ LGEE+R +++G +STSG  LNV+   +   +  K   
Sbjct: 95  MRTTISNSAGKSKLNYDDIRDLILGEEVRRRDAGESSTSGSTLNVETRGHIQANCYKMKK 154

Query: 61  ---GKLRNNRIEGAH-----------NTWVVDSGASFHTTGQRDILENYVAGNHGKMYLA 120
               K  N   E  H           ++WV+DSGASFHTT  ++IL+NYVAG++G +YLA
Sbjct: 155 EDDDKSANTVAEEGHEALLLSVSSPLDSWVLDSGASFHTTPHQNILQNYVAGDYGVVYLA 214

Query: 121 DGKPLDIIGICDVNLKMANSS 126
           DG+PL IIGI DV +K+ N+S
Sbjct: 215 DGEPLRIIGIGDVEIKLPNNS 235

BLAST of Moc04g10630 vs. ExPASy TrEMBL
Match: A0A2P5CH01 (Zinc finger, CCHC-type OS=Parasponia andersonii OX=3476 GN=PanWU01x14_153190 PE=4 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 9.2e-23
Identity = 74/181 (40.88%), Postives = 97/181 (53.59%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNV---DR--ETNNNRDH 60
           M+A +SNS GKEKL+F DVRD  L EE+R  +SG  ++S   LN+   DR  E N+NR  
Sbjct: 159 MRAAVSNSVGKEKLQFNDVRDRILAEEVRRIDSGEGASSSSALNLKNRDRSSEKNSNRGR 218

Query: 61  RKYGKLRNNR-------------------------------------------------- 120
            K  K RN R                                                  
Sbjct: 219 DK-SKSRNGRNKSRSKRRMECWNCGKTGHIKKNCRAPRKDDSRNDANAITDEVQDALLLS 278

Query: 121 IEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLKMANSS 127
           ++   ++WV+DSGASFHTT  RD+LENY+AGN+GK+YLADG+PLDI+G+ D+ LKM+N S
Sbjct: 279 VDNPIDSWVLDSGASFHTTAHRDVLENYIAGNYGKVYLADGEPLDIVGMGDIRLKMSNGS 338

BLAST of Moc04g10630 vs. ExPASy TrEMBL
Match: A5ASH8 (gag_pre-integrs domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_040580 PE=4 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.0e-22
Identity = 57/126 (45.24%), Postives = 81/126 (64.29%), Query Frame = 0

Query: 1   MKATISNSCGKEKLKFADVRDVALGEEIRIKNSGIASTSGIDLNVDRETNNNRDHRKYGK 60
           M+  +SNS GKEKLK+ D+RD+ L EEIR ++ G  S SG DLN++              
Sbjct: 159 MRMAVSNSTGKEKLKYNDIRDLILAEEIRRRDVGETSGSGSDLNLE-------------- 218

Query: 61  LRNNRIEGAHNTWVVDSGASFHTTGQRDILENYVAGNHGKMYLADGKPLDIIGICDVNLK 120
               R++   + WV+DSGASFHTT  R+I++NYVA + GK+YLADG  LD++G+ DV + 
Sbjct: 219 -TRGRVDSPLDDWVLDSGASFHTTPHREIIQNYVADDFGKVYLADGSALDVVGLGDVRIS 269

Query: 121 MANSSV 127
           + N S+
Sbjct: 279 LPNGSI 269

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022152845.13.5e-2445.14uncharacterized protein LOC111020469 [Momordica charantia][more]
KAG6525408.11.3e-2350.78hypothetical protein ZIOFF_015364 [Zingiber officinale][more]
RVX07359.18.5e-2345.74Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Vitis vinifera][more]
KAB5561215.18.5e-2346.81hypothetical protein DKX38_006172 [Salix brachista][more]
PON60333.11.9e-2240.88Zinc finger, CCHC-type [Parasponia andersonii][more]
Match NameE-valueIdentityDescription
P109787.0e-0438.18Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
Match NameE-valueIdentityDescription
A0A6J1DF431.7e-2445.14uncharacterized protein LOC111020469 OS=Momordica charantia OX=3673 GN=LOC111020... [more]
A0A438JEH94.1e-2345.74Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Vitis vinifera OX... [more]
A0A5N5N1664.1e-2346.81Uncharacterized protein OS=Salix brachista OX=2182728 GN=DKX38_006172 PE=4 SV=1[more]
A0A2P5CH019.2e-2340.88Zinc finger, CCHC-type OS=Parasponia andersonii OX=3476 GN=PanWU01x14_153190 PE=... [more]
A5ASH82.0e-2245.24gag_pre-integrs domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_0... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g10630.1Moc04g10630.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005488 binding