Moc04g08300 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g08300
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionL-galactose dehydrogenase
Locationchr4: 5982340 .. 5985626 (+)
RNA-Seq ExpressionMoc04g08300
SyntenyMoc04g08300
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCATCGCATCCCAAGCTCGAGCTCCGCAAGTTGGGAAACACCGGCCTCAAAATCAGCAGTGTGGGCTTCGGTGCCTCGCCTCTTGGCAGCGTCTTCGGCCCCGTTTCTGAGGACGATGCCGTCGCCTCCGTTCGCCAAGCCTTTCGCCTCGGCATTAACTTCTTTGACACCTCCCCGTACGTAATTTCTTTCCCTTTCAATTTGGGGTCCGAATTTTCGCTCTGTTTCTTAACTGGGAAAATCTTGTTGGAGGAATTGGATTCGTTGTTATTGTGTTTGGAGTGAATTTTTAGCCTTAGCTTTCAATTTCTACTTTCTGTTTGAAGAACTATTGAGATGTCTACTGCAAAATTGGTGAGTTTGGGAATTATCCAATGCTTGATCTTCTGCATTCATTTTGTTGCTAGTGTCTAGCTCTAGCACTTAATTTGGGTTTCCCACACTAAACATATGTAAAGGTACTATGGAGGGACGTTATCAGAAAAGATGCTTGGTAAGGGACTGAAGGCTCTAGGAGTTCCAAGGAGTGAGTATGTAGTGTCAACCAAGTGTGGGAGATATGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACTAGGAGCATTGATGAGAGTTTGGCAAGGCTACAACTCGATTACGTCGATATACTGCAATGCCATGATATCGAATTTGGATCTCTCGATCAGGTTGTGACTTATCTGATGCTTTGTTATTTATCTTTAAGGATTGATTGATTTGATTGATTTGATTGACCATTTGTTTCTGTGTTATCAACATGGTTATTTTCATTTATAGTACAAAAGCAAATGTAGATCTTCAGCTGATTAAGTTTATTGCAGGTCGTGAATGAGACGATTCCTGCACTTCAAATGCTGAAGGAATCTGGGAAGATCCATTTCATTGGTATTACAGGGCTTCCATTGGAAATTTTTACGTATGTGCTTGATCGAGTACCTCCTGGCTCCGTTGATGTGATTCTTTCATATTGTCACTACAGCATTAACGATTCAACATTGTTAGATCTGGTACCTTACTTGAAGAGTAAGGGAATTGGAGTAATCACTGCTTCTCCTCTTGCAATGGGACTTCTAACTGATAGTGGTCCTCCAGAATGGCACCCAGCTTCGCCAGAATTGAAGGTTCCATTTCTCCTGCACTTCTCTTTGATTTTGATGCAAAGCAACTACAGTTTCCATAAAAATTTGTTTCATTAGCTTCAAAGTTTAAACTTAACAAGATAGAGCATGGAAAGCTCTCATTCTCAATTGACCAATGTCATGTAAAGAAGGCATCTGTTTGGGGGAGTTCATTTCTCATTCTATCATGGAAATTTTATCTCTTGGAAGAAAATTAAGTTTCTAGGTATTGATAATAGTATGTGGTAAATACTAGGTTGTTTGACCACACAAGCAAACCCAAAACCATTTGCTCTACTTGTGTCTGTGATTTAATCTTTTATTTTTCCCACTCTGTTTTGTTTGAGCTTTAACTTTATACTGAAAGTAATATTGTTCTCTTATTTAAAATTGCTACAGTCTGCATGTCAAGCTGCAGCTGCTCATTGTAAAAAGAAAGGAAAAAATATTTCAAAGTTAGCCCTCCAATACAGCTTGTCAAACAAAGATATTTCAACAGTGCTGGTTGGCATGAACTCTGTCAGACAGGTATTTCAAGAATCTCATTGCTTTTTATTCTTTTTCCATATTACTTTAAATATCTTGAGTTCAATTTTCTTTTCTGAAATTAGTGTATTTTCTCTTTTACGTCTTATACTAATTCCGTATCCATCAGTTGGTAATTTCCTCTTCTAAGCTTATACTTTTGTGCCTATATTATTGTGGCAAAGTATTATCGGGATGCACTTATTTATTACATGTAATAAAAATAAACCAATAATAGTAGTCCATGTAAGAAAGTAAGGAGGAGCTCCTGCTTATAGTAAGGAGATATGTTTTGTTCAATTATGTGAGTTCTACTCAAACAACTACAAAAACAAAAAAAAAAACAAAACGACCTCATAGCAAATTGGAAGGCGTTTTTGTAACTTGCTTATAAGCCCTTTTGTATATTTCATATCATCAATGAAATACATGTTTCTTAATAAAAAAAAAAACAAACAAAACAAGACTTTATTTATCTATATCTTATTCAGCAAAAGACTTTGAATCCTGTCAATGGGTGAGTTTTACAAAGATACTGAATGGAAAGATTAATAAAAAAGAGAACTAGTTGTATCATGCCTACAAGTTCATGGCGCTTCAATAGACCTTGGGATTTCCAGCTTCTTTTTTTGGTAGGAAATAAAAATATTATTAGAAAGAATGACTCAAAGAAGAAAAGTTAAGGAATCGTCCCAGACCAAAGCCTCTAAGGAGATTGCATAGTGACAGTCCATTCCTATTGGCACTGATCAAGGTTGAAGCGCAATTGCAAAAATGTTTGGAATTTAGAAAGAGAGTTCCAAGGAGAGGTTAATAGAGTTTCTTGGTGTATGAGAAGCTTTTTTTTTTTTCTTTTTTTAACCTTAAAGAAGTATGAGGATATTTTCTAAAAGGGATTTTCTCATACTAACAGTGTCCAAACTGGCTCCTGATGAGCCTCCTTTGATGGATCCTGTAAACATCAATCTTTGTGAGAAGGTTGTATCTGTAGGTTTCAACAGGCAGGGATTTTCTTTGTTTTAATTACTGAATGCAAAATACTGACTGATGCCAAGGTTTTCAATATCATGTTGATTGAAATGTCAAGGTCTCAATTTCATGAAAATATCGATAAAATGTTGGTGTCAATGGATATTTCTGTAAAAGTTAACGAAATTAAAAAATCGAAAAAATATTTAAATTAACAAATAAATATTTTTCTATTTCCAATTATTCGTATTAATATTATGTATATATTAGTGACATTTTGTTGATATTTTTCATATTTTGTCGATTTTTTTGTAATATGATAGAAATGTTGATTTACCTTCGTGTCGATGTCAAACCCATGGATACATGGAAACGTCGATGGACATGTCAACGTGTCGATGGATATTTAATATCATGACTGATGCCATGAATTGATAGTAGCCGAACACAACTTTTTCTCTCATGCTCAAAATCTAAAGAGAATGCATGTTAACCAAGTTGTAGCAATTTGAAATCAGGTGGAGCAAAATGTTGCAGCTGCTGAAGAACTTGCAACATTTGGGAGGGACGAGGAAACTCTATCAGAGGTTGAAGCTATCCTTGAACCTGTCAAGAACCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

mRNA sequence

ATGGCGGCATCGCATCCCAAGCTCGAGCTCCGCAAGTTGGGAAACACCGGCCTCAAAATCAGCAGTGTGGGCTTCGGTGCCTCGCCTCTTGGCAGCGTCTTCGGCCCCGTTTCTGAGGACGATGCCGTCGCCTCCGTTCGCCAAGCCTTTCGCCTCGGCATTAACTTCTTTGACACCTCCCCGTACTATGGAGGGACGTTATCAGAAAAGATGCTTGGTAAGGGACTGAAGGCTCTAGGAGTTCCAAGGAGTGAGTATGTAGTGTCAACCAAGTGTGGGAGATATGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACTAGGAGCATTGATGAGAGTTTGGCAAGGCTACAACTCGATTACGTCGATATACTGCAATGCCATGATATCGAATTTGGATCTCTCGATCAGGTCGTGAATGAGACGATTCCTGCACTTCAAATGCTGAAGGAATCTGGGAAGATCCATTTCATTGGTATTACAGGGCTTCCATTGGAAATTTTTACGTATGTGCTTGATCGAGTACCTCCTGGCTCCGTTGATGTGATTCTTTCATATTGTCACTACAGCATTAACGATTCAACATTGTTAGATCTGGTACCTTACTTGAAGAGTAAGGGAATTGGAGTAATCACTGCTTCTCCTCTTGCAATGGGACTTCTAACTGATAGTGGTCCTCCAGAATGGCACCCAGCTTCGCCAGAATTGAAGTCTGCATGTCAAGCTGCAGCTGCTCATTGTAAAAAGAAAGGAAAAAATATTTCAAAGTTAGCCCTCCAATACAGCTTGTCAAACAAAGATATTTCAACAGTGCTGGTTGGCATGAACTCTGTCAGACAGGTGGAGCAAAATGTTGCAGCTGCTGAAGAACTTGCAACATTTGGGAGGGACGAGGAAACTCTATCAGAGGTTGAAGCTATCCTTGAACCTGTCAAGAACCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

Coding sequence (CDS)

ATGGCGGCATCGCATCCCAAGCTCGAGCTCCGCAAGTTGGGAAACACCGGCCTCAAAATCAGCAGTGTGGGCTTCGGTGCCTCGCCTCTTGGCAGCGTCTTCGGCCCCGTTTCTGAGGACGATGCCGTCGCCTCCGTTCGCCAAGCCTTTCGCCTCGGCATTAACTTCTTTGACACCTCCCCGTACTATGGAGGGACGTTATCAGAAAAGATGCTTGGTAAGGGACTGAAGGCTCTAGGAGTTCCAAGGAGTGAGTATGTAGTGTCAACCAAGTGTGGGAGATATGCTGAGGGTTTTGATTTCAGTGCTGAGAGGGTGACTAGGAGCATTGATGAGAGTTTGGCAAGGCTACAACTCGATTACGTCGATATACTGCAATGCCATGATATCGAATTTGGATCTCTCGATCAGGTCGTGAATGAGACGATTCCTGCACTTCAAATGCTGAAGGAATCTGGGAAGATCCATTTCATTGGTATTACAGGGCTTCCATTGGAAATTTTTACGTATGTGCTTGATCGAGTACCTCCTGGCTCCGTTGATGTGATTCTTTCATATTGTCACTACAGCATTAACGATTCAACATTGTTAGATCTGGTACCTTACTTGAAGAGTAAGGGAATTGGAGTAATCACTGCTTCTCCTCTTGCAATGGGACTTCTAACTGATAGTGGTCCTCCAGAATGGCACCCAGCTTCGCCAGAATTGAAGTCTGCATGTCAAGCTGCAGCTGCTCATTGTAAAAAGAAAGGAAAAAATATTTCAAAGTTAGCCCTCCAATACAGCTTGTCAAACAAAGATATTTCAACAGTGCTGGTTGGCATGAACTCTGTCAGACAGGTGGAGCAAAATGTTGCAGCTGCTGAAGAACTTGCAACATTTGGGAGGGACGAGGAAACTCTATCAGAGGTTGAAGCTATCCTTGAACCTGTCAAGAACCAGACATGGCCAAGTGGAATCCAACAGAGCTGA

Protein sequence

MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSVDVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSACQAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEETLSEVEAILEPVKNQTWPSGIQQS
Homology
BLAST of Moc04g08300 vs. NCBI nr
Match: XP_022155328.1 (L-galactose dehydrogenase [Momordica charantia])

HSP 1 Score: 636.0 bits (1639), Expect = 1.8e-178
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS
Sbjct: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV
Sbjct: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAILEPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILEPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. NCBI nr
Match: KAG7011583.1 (L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 601.3 bits (1549), Expect = 5.0e-168
Identity = 302/323 (93.50%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 47  MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 106

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 107 PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 166

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQVVNETIPALQ L+E+GKI FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 167 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 226

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 227 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 286

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAAHCKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 287 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 346

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 347 LSEVEAILHPVKNQTWPSGIQQS 369

BLAST of Moc04g08300 vs. NCBI nr
Match: XP_022952291.1 (L-galactose dehydrogenase [Cucurbita moschata])

HSP 1 Score: 601.3 bits (1549), Expect = 5.0e-168
Identity = 302/323 (93.50%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQVVNETIPALQ L+E+GKI FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAAHCKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. NCBI nr
Match: KAG6571895.1 (L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 598.6 bits (1542), Expect = 3.2e-167
Identity = 301/323 (93.19%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQVVNETIPALQ L+E+GKI FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
            AAAAHCKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 LAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. NCBI nr
Match: XP_023511604.1 (L-galactose dehydrogenase isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597.0 bits (1538), Expect = 9.4e-167
Identity = 301/323 (93.19%), Postives = 314/323 (97.21%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQVVNETIPALQ L+E+GKI FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAA CKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAAALCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. ExPASy Swiss-Prot
Match: O81884 (L-galactose dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=LGALDH PE=1 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 2.0e-151
Identity = 263/317 (82.97%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 7   KLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGGT 66
           K+ELR LGNTGLK+S+VGFGASPLGSVFGPV+EDDAVA+VR+AFRLGINFFDTSPYYGGT
Sbjct: 3   KIELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGT 62

Query: 67  LSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILQ 126
           LSEKMLGKGLKAL VPRS+Y+V+TKCGRY EGFDFSAERV +SIDESL RLQLDYVDIL 
Sbjct: 63  LSEKMLGKGLKALQVPRSDYIVATKCGRYKEGFDFSAERVRKSIDESLERLQLDYVDILH 122

Query: 127 CHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSVDVILSY 186
           CHDIEFGSLDQ+V+ETIPALQ LK+ GK  FIGITGLPL+IFTYVLDRVPPG+VDVILSY
Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182

Query: 187 CHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSACQAAAAH 246
           CHY +NDSTLLDL+PYLKSKG+GVI+ASPLAMGLLT+ GPPEWHPASPELKSA +AA AH
Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242

Query: 247 CKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEETLSEVEA 306
           CK KGK I+KLALQYSL+NK+IS+VLVGM+SV QVE+NVAA  EL + G D+ETLSEVEA
Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302

Query: 307 ILEPVKNQTWPSGIQQS 324
           ILEPVKN TWPSGI Q+
Sbjct: 303 ILEPVKNLTWPSGIHQN 319

BLAST of Moc04g08300 vs. ExPASy Swiss-Prot
Match: Q04212 (D-arabinose 1-dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ARA2 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 9.5e-29
Identity = 92/292 (31.51%), Postives = 156/292 (53.42%), Query Frame = 0

Query: 46  VRQAFRLGINFFDTSPYYGGTLSEKMLGKGLKAL--GVPRSEYVVSTKCGRY-AEGFDFS 105
           ++ AF  GIN  DTSPYYG   SE + G+ L  L    PR  Y + TK GR  AE F++S
Sbjct: 41  IKYAFSHGINAIDTSPYYGP--SEVLYGRALSNLRNEFPRDTYFICTKVGRIGAEEFNYS 100

Query: 106 AERVTRSIDESLARLQLDYVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITG 165
            + V  S+  S  RL   Y+D++  HD+EF     ++ E +  L+ LK  G I   GI+G
Sbjct: 101 RDFVRFSVHRSCERLHTTYLDLVYLHDVEFVKFPDIL-EALKELRTLKNKGVIKNFGISG 160

Query: 166 LPLEIFTYVLDRVPP-----GSVDVILSYCHYSINDSTLLDLVPYL--KSKGIGVITASP 225
            P++  T++ +         GS+D +LSYC+ ++ ++ LL+    L   +K   V  AS 
Sbjct: 161 YPIDFITWLAEYCSTEESDIGSLDAVLSYCNLNLQNNKLLNFRERLLRNAKLKMVCNASI 220

Query: 226 LAMGLLTDSGPPEWHPASPELKSACQAAAAHCKKKGKNISKLALQYSLSN-KDISTVLVG 285
           L+M LL      ++HP S EL+     AA +C+++  +++ LA +Y++S       V++G
Sbjct: 221 LSMSLLRSQETRQFHPCSHELRECASQAAKYCQEQNVDLADLATRYAISEWVGKGPVVLG 280

Query: 286 MNSVRQVEQNVAAAEELATFG-----RDEETLSEVEA-ILEPVKNQTWPSGI 321
           ++S+ +++  +   E + + G     +D + +  ++  I +   N+ W SGI
Sbjct: 281 VSSMEELKLALDNYEIVKSNGNRLSSKDGQLVEYIQKNIFKEHFNEEWSSGI 329

BLAST of Moc04g08300 vs. ExPASy Swiss-Prot
Match: Q52472 (D-threo-aldose 1-dehydrogenase OS=Pseudomonas sp. OX=306 GN=fdh PE=1 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.2e-26
Identity = 91/302 (30.13%), Postives = 143/302 (47.35%), Query Frame = 0

Query: 17  GLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGGTLSEKMLGKGL 76
           GL I ++G+GA+ +G++F  +S+D+A A +  A+  GI ++DT+P+YG  LSEK LG  L
Sbjct: 12  GLAIPALGYGAANVGNLFRALSDDEAWAVLEAAWDAGIRYYDTAPHYGLGLSEKRLGAFL 71

Query: 77  KALGVPRSEYVVSTKCGR------------------------YAEGFDFSAERVTRSIDE 136
           +    PR E+VVSTK GR                            +DF+ + +  SI E
Sbjct: 72  QT--KPRDEFVVSTKAGRLLRPNPERRPSGLDTDNDFHVPDDLRREWDFTEQGIRASIAE 131

Query: 137 SLARLQLDYVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVL 196
           S  RL LD +D+L  HD E   LD  +    PAL+ ++  G +  IGI  +  +  T   
Sbjct: 132 SQERLGLDRIDLLYLHDPERHDLDLALASAFPALEKVRAEGVVKAIGIGSMVSDALTRA- 191

Query: 197 DRVPPGSVDVILSYCHYS-INDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPP---- 256
             V    +D+I+    Y+ +      +++P       G++ AS    GLL  S P     
Sbjct: 192 --VREADLDLIMVAGRYTLLEQPAATEVLPACAENATGIVAASVFNSGLLAQSEPKRDGR 251

Query: 257 -EWHPASPELKSACQAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVA 289
            E+     EL       AA C+     +   A+Q+ L +  + +V+VG +   Q+ QN  
Sbjct: 252 YEYGQLPDELWDRLVRIAAICRNHDVPLPAAAIQFPLQSALVRSVVVGGSRPAQLTQNAE 308

BLAST of Moc04g08300 vs. ExPASy Swiss-Prot
Match: Q76KC2 (Pyridoxal 4-dehydrogenase OS=Microbacterium luteolum OX=69367 GN=pld1 PE=1 SV=3)

HSP 1 Score: 102.1 bits (253), Expect = 1.2e-20
Identity = 87/325 (26.77%), Postives = 143/325 (44.00%), Query Frame = 0

Query: 9   ELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGGTLS 68
           E R LG TGL ++++G G +PLG ++ PVS  DA A +   +  GI +FD++P YG    
Sbjct: 7   EKRALGRTGLTVTALGLGTAPLGGLYAPVSRADADALLEAGWDSGIRYFDSAPMYGYGRC 66

Query: 69  EKMLGKGLKALGVPRSEYVVSTKCGR-----------------------------YAEGF 128
           E +LG  L+         V+STK GR                             + E F
Sbjct: 67  EHLLGDMLRE---KPERAVISTKVGRLMTNERAGRTLPPAPPKNPLDSGWHNGLNFREVF 126

Query: 129 DFSAERVTRSIDESLARLQLDYVDILQCHDI---------EFGSLDQVVNETIPALQMLK 188
           D+S + V RS D+S  RL    +D+L  HDI         EF            AL  L+
Sbjct: 127 DYSYDGVMRSFDDSQQRLGFPEIDLLYVHDIGRVTHADRHEFHWNALTRGGGFRALTELR 186

Query: 189 ESGKIHFIGITGLPLEIFTYVLDRVPPGSVDVILSYCHYSINDS-TLLDLVPYLKSKGIG 248
            +G I      GL +  +  + D +    +D  L    YS+ D  +  + +P  + +G+ 
Sbjct: 187 AAGNIKGF---GLGVNEWQIIRDALEEADLDCSLLAGRYSLLDQVSEKEFLPLAQKRGMA 246

Query: 249 VITASPLAMGLLT--DSGPPEWHPASPELKSACQAAAAH--CKKKGKNISKLALQYSLSN 291
           ++ A     G+L     G  ++  A    +   +    H  C +    ++  A+Q+ L +
Sbjct: 247 LVIAGVFNSGILAAPRGGEQKFDYADAPAEIIARTNRLHDICDEYHVPLAAAAMQFPLRH 306

BLAST of Moc04g08300 vs. ExPASy Swiss-Prot
Match: M2YMU7 (Aldo-keto reductase MYCFIDRAFT_87468 OS=Pseudocercospora fijiensis (strain CIRAD86) OX=383855 GN=MYCFIDRAFT_87468 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 2.3e-19
Identity = 90/330 (27.27%), Postives = 153/330 (46.36%), Query Frame = 0

Query: 11  RKLGNTGLKISSVGFGASPLGS---VFGPVSEDDAVASVRQAFRLGINFFDTSPYYGGTL 70
           ++LG TGL++S    G   LG    V   + ED A+  ++ AF LG+N +DT+  Y    
Sbjct: 38  KRLGKTGLRVSVPILGCMSLGDKHWVPWALEEDKALPLLKGAFDLGLNCWDTANVYSNGK 97

Query: 71  SEKMLGKGLKALGVPRSEYVVSTKCG------------RYAEGFDFSAERVTR------- 130
           SE+++GK +K   +PR + V+ +KCG            RY      S + V +       
Sbjct: 98  SEEIIGKAIKTYQLPRHKLVLLSKCGGYVGEAPNVKGDRYENAISRSKDYVNQGGLSRSA 157

Query: 131 ---SIDESLARLQLDYVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPL 190
               ++ SL RL   Y+D+LQ H  +    +  + ET+ AL  L   GK+ ++G   +  
Sbjct: 158 IFEQVEASLRRLDTTYLDVLQIHRFD---PNTPIEETMKALHDLVSIGKVRYLGACSM-- 217

Query: 191 EIFTYVLDRV-----PPGSVDVILSYCHYS-INDSTLLDLVPYLKSKGIGVITASPLAMG 250
             +TY   ++       G    +    HYS +      D++ +    G+G+I  +PLA G
Sbjct: 218 --YTYQFAQMQFVAEKNGWTKFVSMQNHYSLLYREEERDMIRFCNETGVGLIPWAPLASG 277

Query: 251 LLTDSGPPEWHPASPELKSACQAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVR 310
           +LT     +       + +  Q  A   +K+G  +S +AL  S  NK +S  ++  + + 
Sbjct: 278 MLTRPATQKVPEGDRAIINRVQEVA---EKRGWRMSYVAL--SWINKRVSAPVIDFDRID 337

BLAST of Moc04g08300 vs. ExPASy TrEMBL
Match: A0A6J1DMP0 (L-galactose dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111022462 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 8.8e-179
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS
Sbjct: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV
Sbjct: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAILEPVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILEPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. ExPASy TrEMBL
Match: A0A6J1GJU5 (L-galactose dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111455013 PE=4 SV=1)

HSP 1 Score: 601.3 bits (1549), Expect = 2.4e-168
Identity = 302/323 (93.50%), Postives = 315/323 (97.52%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFGPVSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGPVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQVVNETIPALQ L+E+GKI FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLREAGKIRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAAHCKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. ExPASy TrEMBL
Match: A0A6J1IA20 (L-galactose dehydrogenase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471025 PE=4 SV=1)

HSP 1 Score: 596.7 bits (1537), Expect = 5.9e-167
Identity = 301/323 (93.19%), Postives = 313/323 (96.90%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MAAS+PKL+LRKLGNTGLK SSVGFGASPLGSVFG VSE+DAVASVR+AFRLGINFFDTS
Sbjct: 1   MAASYPKLQLRKLGNTGLKTSSVGFGASPLGSVFGLVSEEDAVASVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYGGTLSEKMLGKGLKAL VPRSEY+VSTKCGRYAEGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGGTLSEKMLGKGLKALDVPRSEYIVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQVVNETIPALQ LKE+GK  FIGITGLPLEIFTYVLDRVPPGS+
Sbjct: 121 YVDILHCHDIEFGSLDQVVNETIPALQKLKEAGKTRFIGITGLPLEIFTYVLDRVPPGSI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSK IG+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKEIGIISASPLAMGLLTDYGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAAAHCKKKGK+ISKLALQYSLSNKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAAAHCKKKGKHISKLALQYSLSNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQQS
Sbjct: 301 LSEVEAILHPVKNQTWPSGIQQS 323

BLAST of Moc04g08300 vs. ExPASy TrEMBL
Match: A0A1S3C0W3 (L-galactose dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103495364 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 287/323 (88.85%), Postives = 307/323 (95.05%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MA S+PKL LR+LGNTGLK SSVGFGASPLGSVF PVSE+DAVA+VR+AFRLGINFFDTS
Sbjct: 1   MAVSYPKLPLRELGNTGLKTSSVGFGASPLGSVFSPVSEEDAVAAVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG  LSEKMLGKGLKALGVPRSEY+VSTKCGRY EGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGRNLSEKMLGKGLKALGVPRSEYIVSTKCGRYGEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQ+VNETIPALQ LKE+GK  F+GITGLPLEIFTYVLDRVPPG++
Sbjct: 121 YVDILHCHDIEFGSLDQIVNETIPALQKLKEAGKTRFLGITGLPLEIFTYVLDRVPPGTI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSKG+G+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKGVGIISASPLAMGLLTDQGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAA HC+KKG+NI+KLA+QYSL NKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAADHCRKKGRNITKLAIQYSLVNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQ S
Sbjct: 301 LSEVEAILNPVKNQTWPSGIQNS 323

BLAST of Moc04g08300 vs. ExPASy TrEMBL
Match: A0A5A7SPF2 (L-galactose dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001760 PE=4 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.6e-162
Identity = 287/323 (88.85%), Postives = 307/323 (95.05%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTS 60
           MA S+PKL LR+LGNTGLK SSVGFGASPLGSVF PVSE+DAVA+VR+AFRLGINFFDTS
Sbjct: 1   MAVSYPKLPLRELGNTGLKTSSVGFGASPLGSVFSPVSEEDAVAAVREAFRLGINFFDTS 60

Query: 61  PYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLD 120
           PYYG  LSEKMLGKGLKALGVPRSEY+VSTKCGRY EGFDFSAERVTRSIDESLARLQLD
Sbjct: 61  PYYGRNLSEKMLGKGLKALGVPRSEYIVSTKCGRYGEGFDFSAERVTRSIDESLARLQLD 120

Query: 121 YVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSV 180
           YVDIL CHDIEFGSLDQ+VNETIPALQ LKE+GK  F+GITGLPLEIFTYVLDRVPPG++
Sbjct: 121 YVDILHCHDIEFGSLDQIVNETIPALQKLKEAGKTRFLGITGLPLEIFTYVLDRVPPGTI 180

Query: 181 DVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSAC 240
           DVILSYCHYSINDSTLLDL+PYLKSKG+G+I+ASPLAMGLLTD GPPEWHPASPELKSAC
Sbjct: 181 DVILSYCHYSINDSTLLDLLPYLKSKGVGIISASPLAMGLLTDQGPPEWHPASPELKSAC 240

Query: 241 QAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEET 300
           QAAA HC+KKG+NI+KLA+QYSL NKDISTVLVGMNSVRQVE+NVAAAEELATFGRDEET
Sbjct: 241 QAAADHCRKKGRNITKLAIQYSLVNKDISTVLVGMNSVRQVEENVAAAEELATFGRDEET 300

Query: 301 LSEVEAILEPVKNQTWPSGIQQS 324
           LSEVEAIL PVKNQTWPSGIQ S
Sbjct: 301 LSEVEAILNPVKNQTWPSGIQNS 323

BLAST of Moc04g08300 vs. TAIR 10
Match: AT4G33670.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 536.6 bits (1381), Expect = 1.4e-152
Identity = 263/317 (82.97%), Postives = 293/317 (92.43%), Query Frame = 0

Query: 7   KLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSPYYGGT 66
           K+ELR LGNTGLK+S+VGFGASPLGSVFGPV+EDDAVA+VR+AFRLGINFFDTSPYYGGT
Sbjct: 3   KIELRALGNTGLKVSAVGFGASPLGSVFGPVAEDDAVATVREAFRLGINFFDTSPYYGGT 62

Query: 67  LSEKMLGKGLKALGVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLARLQLDYVDILQ 126
           LSEKMLGKGLKAL VPRS+Y+V+TKCGRY EGFDFSAERV +SIDESL RLQLDYVDIL 
Sbjct: 63  LSEKMLGKGLKALQVPRSDYIVATKCGRYKEGFDFSAERVRKSIDESLERLQLDYVDILH 122

Query: 127 CHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVPPGSVDVILSY 186
           CHDIEFGSLDQ+V+ETIPALQ LK+ GK  FIGITGLPL+IFTYVLDRVPPG+VDVILSY
Sbjct: 123 CHDIEFGSLDQIVSETIPALQKLKQEGKTRFIGITGLPLDIFTYVLDRVPPGTVDVILSY 182

Query: 187 CHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASPELKSACQAAAAH 246
           CHY +NDSTLLDL+PYLKSKG+GVI+ASPLAMGLLT+ GPPEWHPASPELKSA +AA AH
Sbjct: 183 CHYGVNDSTLLDLLPYLKSKGVGVISASPLAMGLLTEQGPPEWHPASPELKSASKAAVAH 242

Query: 247 CKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEETLSEVEA 306
           CK KGK I+KLALQYSL+NK+IS+VLVGM+SV QVE+NVAA  EL + G D+ETLSEVEA
Sbjct: 243 CKSKGKKITKLALQYSLANKEISSVLVGMSSVSQVEENVAAVTELESLGMDQETLSEVEA 302

Query: 307 ILEPVKNQTWPSGIQQS 324
           ILEPVKN TWPSGI Q+
Sbjct: 303 ILEPVKNLTWPSGIHQN 319

BLAST of Moc04g08300 vs. TAIR 10
Match: AT1G60750.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 100.5 bits (249), Expect = 2.6e-21
Identity = 94/317 (29.65%), Postives = 145/317 (45.74%), Query Frame = 0

Query: 1   MAASHPKLELRKLGNTGLKISSVGFGASPLGSVFG-PVSEDDAVASVRQAFRLGINFFDT 60
           MA    ++   KLG+ GL++S+ G G   L   +G P  E +AVA +R A   G+ F DT
Sbjct: 1   MAEEACQVRRMKLGSQGLEVSAQGLGCMGLSDFYGAPTPETNAVALLRHAINAGVTFLDT 60

Query: 61  SPYYGGTLSEKMLGKGLKALGVPRSEYVVSTKCGRYAE-----GFDFSAERVTRSIDESL 120
           S  YG   +E +LGK LK  G+ R +  ++TK G  A      GF    E V  + + SL
Sbjct: 61  SDIYGPETNELLLGKALKD-GL-RDKVELATKFGITASEDGKFGFRGDPEYVRIACEASL 120

Query: 121 ARLQLDYVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDR 180
            RL +  +D+   H I+       +  TI  L+ L E GKI +IG++             
Sbjct: 121 KRLGVTCIDLYYQHRID---TTLPIEITIGELKKLVEEGKIKYIGLSEASASTIRRAHAV 180

Query: 181 VPPGSVDVILSYCHYSINDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASP 240
            P  +V +  S     + +    D++P  +  GIG++  SPL  G L   G P +   + 
Sbjct: 181 HPITAVQIEWSLWSRDVEE----DIIPTCRELGIGIVAYSPLGRGFL---GLPRFQQENL 240

Query: 241 E-LKSACQAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELAT 300
           E  K   +   A   KK    ++LAL +     D    + G + ++ + QN+ A     T
Sbjct: 241 ENNKILYEKVQAMATKKSCTPAQLALAWVHHQGDDVCPIPGTSKIQNLNQNIGALSVKLT 300

Query: 301 FGRDEETLSEVEAILEP 311
                E + E+EAI +P
Sbjct: 301 ----PEEMVELEAIAQP 301

BLAST of Moc04g08300 vs. TAIR 10
Match: AT1G04690.1 (potassium channel beta subunit 1 )

HSP 1 Score: 95.1 bits (235), Expect = 1.1e-19
Identity = 81/311 (26.05%), Postives = 142/311 (45.66%), Query Frame = 0

Query: 8   LELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFR-LGINFFDTSPYYGGT 67
           ++ + LG +GLK+S++ FGA      FG   +     S+ Q  R  G+NFFD +  Y   
Sbjct: 1   MQYKNLGKSGLKVSTLSFGA---WVTFGNQLDVKEAKSILQCCRDHGVNFFDNAEVYANG 60

Query: 68  LSEKMLGKGLKALGVPRSEYVVSTKC---GRYAEGFDFSAERVTRSIDESLARLQLDYVD 127
            +E+++G+ ++ LG  RS+ V+STK    G        S + +      SL RL +DYVD
Sbjct: 61  RAEEIMGQAIRELGWRRSDIVISTKIFWGGPGPNDKGLSRKHIVEGTKASLKRLDMDYVD 120

Query: 128 ILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYV------LDRVPP 187
           +L CH  +  +    + ET+ A+  + + G   + G +    +  T        LD V P
Sbjct: 121 VLYCHRPDAST---PIEETVRAMNYVIDKGWAFYWGTSEWSAQQITEAWGAADRLDLVGP 180

Query: 188 GSVDVILSYCHYSI--NDSTLLDLVPYLKSKGIGVITASPLAMGLLT-----DSGPPEWH 247
                I+    Y++        + +P   + GIG+ T SPLA G+LT      + P +  
Sbjct: 181 -----IVEQPEYNMFARHKVETEFLPLYTNHGIGLTTWSPLASGVLTGKYNKGAIPSDSR 240

Query: 248 PASPELKSACQAAAAH------------CKKKGKNISKLALQYSLSNKDISTVLVGMNSV 290
            A    K+    +                 + G  +++LA+ +  SN ++S+V+ G    
Sbjct: 241 FALENYKNLANRSLVDDVLRKVSGLKPIADELGVTLAQLAIAWCASNPNVSSVITGATRE 300

BLAST of Moc04g08300 vs. TAIR 10
Match: AT1G04420.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 63.9 bits (154), Expect = 2.7e-10
Identity = 89/374 (23.80%), Postives = 162/374 (43.32%), Query Frame = 0

Query: 2   AASHPKLELRKLGNTGLKISSVGFGASPLGSVFGPVSEDDAVASVRQAFRLGINFFDTSP 61
           A++   +E RKLG++ L IS V  G    G      +E ++   +  A   GIN  DT+ 
Sbjct: 50  ASTKNAMEYRKLGDSDLNISEVTMGTMTFGE---QNTEKESHEMLSYAIEEGINCIDTAE 109

Query: 62  YYGGTLSEKMLGK-------GLKALGVPRSEYVVSTKCGRYAEGFDF----------SAE 121
            Y   + ++  GK        LK+    R + V++TK   Y+E   +           A 
Sbjct: 110 AYPIPMKKETQGKTDLYISSWLKS--QQRDKIVLATKVCGYSERSAYIRDSGEILRVDAA 169

Query: 122 RVTRSIDESLARLQLDYVDILQCHDIE-----FGSLDQVVN---------ETIPALQMLK 181
            +  S+++SL RL  DY+D+LQ H  +     FG      +         E + A Q L 
Sbjct: 170 NIKESVEKSLKRLGTDYIDLLQIHWPDRYVPLFGDFYYETSKWRPSVPFAEQLRAFQDLI 229

Query: 182 ESGKIHFIGIT-----GLPLEIFTYVLDRVPPGSVDV-----ILSYCHYSINDSTLLDLV 241
             GK+ +IG++     G+   + T  L+ +P   V +     +L  C Y ++    L  V
Sbjct: 230 VEGKVRYIGVSNETSYGVTEFVNTAKLEGLPK-IVSIQNGYSLLVRCRYEVD----LVEV 289

Query: 242 PYLKSKGIGVITASPLAMG------LLTDS------------GPPEWHPASPELKSACQA 301
            + K+  +G++  SPL  G      L TD             G  E +  S   ++  Q 
Sbjct: 290 CHPKNCNVGLLAYSPLGGGSLSGKYLATDQEATKNARLNLFPGYMERYKGSLAKEATIQY 349

Query: 302 AAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAAAEELATFGRDEETLS 317
                KK G    +LAL +      +++ ++G  SV+Q+++++ A   +      +E ++
Sbjct: 350 VEV-AKKYGLTPVELALGFVRDRPFVTSTIIGATSVKQLKEDIDAF-LMTERPFSQEVMA 409

BLAST of Moc04g08300 vs. TAIR 10
Match: AT1G06690.1 (NAD(P)-linked oxidoreductase superfamily protein )

HSP 1 Score: 63.2 bits (152), Expect = 4.5e-10
Identity = 79/325 (24.31%), Postives = 138/325 (42.46%), Query Frame = 0

Query: 12  KLGNTGLKISSVGFGASPLG--SVFGPVSEDD-AVASVRQAFRL----GINFFDTSPYYG 71
           KLG + LK++ +G G    G  S +     DD  + + + AF      GI+FFDT+  YG
Sbjct: 50  KLGGSDLKVTKLGIGVWSWGDNSYWNDFQWDDRKLKAAKGAFDTSLDNGIDFFDTAEVYG 109

Query: 72  GTL------SEKMLGKGLKAL--GVPRSEYVVSTKCGRYAEGFDFSAERVTRSIDESLAR 131
                    SE +LG+ ++      P +E  V+TK    A  + F  E V  ++ +SL+R
Sbjct: 110 SKFSLGAISSETLLGRFIRERKERYPGAEVSVATKFA--ALPWRFGRESVVTALKDSLSR 169

Query: 132 LQLDYVDILQCHDIEFGSLDQVVNETIPALQMLKESGKIHFIGITGLPLEIFTYVLDRVP 191
           L+L  VD+ Q H       +  ++    A+    E G +  +G++    +      +R+ 
Sbjct: 170 LELSSVDLYQLHWPGLWGNEGYLDGLGDAV----EQGLVKAVGVSNYSEKRLRDAYERLK 229

Query: 192 PGSVDVILSYCHYSI--NDSTLLDLVPYLKSKGIGVITASPLAMGLLTDSGPPEWHPASP 251
              + +  +  +YS+         +       G+ +I  SP+A G LT    PE  P+ P
Sbjct: 230 KRGIPLASNQVNYSLIYRAPEQTGVKAACDELGVTLIAYSPIAQGALTGKYTPENPPSGP 289

Query: 252 -------ELKSACQAAAAHCKKKGKNISKLALQYSLSNKDISTVLVGMNSVRQVEQNVAA 311
                  E  +  Q      K+ G+N SK   Q +L+       ++ +   +  EQ    
Sbjct: 290 RGRIYTREFLTKLQPLLNRIKQIGENYSKTPTQIALNWLVAQGNVIPIPGAKNAEQAKEF 349

Query: 312 AEELATFGRDEETLSEVEAILEPVK 313
           A  +     D E +SE+ ++   +K
Sbjct: 350 AGAIGWSLTDNE-VSELRSLASEIK 367

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155328.11.8e-178100.00L-galactose dehydrogenase [Momordica charantia][more]
KAG7011583.15.0e-16893.50L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022952291.15.0e-16893.50L-galactose dehydrogenase [Cucurbita moschata][more]
KAG6571895.13.2e-16793.19L-galactose dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023511604.19.4e-16793.19L-galactose dehydrogenase isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
O818842.0e-15182.97L-galactose dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=LGALDH PE=1 SV=1[more]
Q042129.5e-2931.51D-arabinose 1-dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S2... [more]
Q524721.2e-2630.13D-threo-aldose 1-dehydrogenase OS=Pseudomonas sp. OX=306 GN=fdh PE=1 SV=1[more]
Q76KC21.2e-2026.77Pyridoxal 4-dehydrogenase OS=Microbacterium luteolum OX=69367 GN=pld1 PE=1 SV=3[more]
M2YMU72.3e-1927.27Aldo-keto reductase MYCFIDRAFT_87468 OS=Pseudocercospora fijiensis (strain CIRAD... [more]
Match NameE-valueIdentityDescription
A0A6J1DMP08.8e-179100.00L-galactose dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111022462 PE=4 SV... [more]
A0A6J1GJU52.4e-16893.50L-galactose dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111455013 PE=4 SV=... [more]
A0A6J1IA205.9e-16793.19L-galactose dehydrogenase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471025... [more]
A0A1S3C0W32.6e-16288.85L-galactose dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103495364 PE=4 SV=1[more]
A0A5A7SPF22.6e-16288.85L-galactose dehydrogenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
Match NameE-valueIdentityDescription
AT4G33670.11.4e-15282.97NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G60750.12.6e-2129.65NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G04690.11.1e-1926.05potassium channel beta subunit 1 [more]
AT1G04420.12.7e-1023.80NAD(P)-linked oxidoreductase superfamily protein [more]
AT1G06690.14.5e-1024.31NAD(P)-linked oxidoreductase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023210NADP-dependent oxidoreductase domainPFAMPF00248Aldo_ket_redcoord: 23..289
e-value: 2.3E-57
score: 194.4
IPR036812NADP-dependent oxidoreductase domain superfamilyGENE3D3.20.20.100coord: 8..319
e-value: 7.7E-76
score: 257.1
IPR036812NADP-dependent oxidoreductase domain superfamilySUPERFAMILY51430NAD(P)-linked oxidoreductasecoord: 8..309
NoneNo IPR availablePIRSRPIRSR000097-1PIRSR000097-1coord: 18..218
e-value: 2.0E-17
score: 60.9
NoneNo IPR availablePIRSRPIRSR000097-2PIRSR000097-2coord: 18..218
e-value: 2.0E-17
score: 60.9
NoneNo IPR availablePIRSRPIRSR000097-3PIRSR000097-3coord: 18..218
e-value: 2.0E-17
score: 60.9
IPR020471Aldo-keto reductasePANTHERPTHR42686GH17980P-RELATEDcoord: 6..320
IPR044479L-galactose dehydrogenase-likeCDDcd19163AKR_galDHcoord: 8..290
e-value: 1.04741E-180
score: 499.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g08300.1Moc04g08300.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
molecular_function GO:0047834 D-threo-aldose 1-dehydrogenase activity
molecular_function GO:0010349 L-galactose dehydrogenase activity
molecular_function GO:0050235 pyridoxal 4-dehydrogenase activity
molecular_function GO:0016491 oxidoreductase activity