Moc04g07330 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g07330
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	GRF-type domain-containing protein
Location	chr4: 5190425 .. 5190982 (+)
RNA-Seq Expression	Moc04g07330
Synteny	Moc04g07330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTACTTCGGAAATTTTTGAAGAATCTGAACTAGAATGTTACTGTGGACTGCCTGCAAAACTGAGAACATCTCAGACACCCAAAAATCCATTCAGATTATTCTACAACTGTCCAAAGGAAATATCTCAAGTAAGTATGTATATTATACCCATGCTCCATTTGCTCATCCAATTTAAGAACTATTTCTCGTTGCTATACTGTACTTAGAGTTTATGTCCTGCACACTACCTGCAGCAATGTGGTTTTTTTCATTGGGCTGATGAACCCGAACCATCTGATGATAGACACGTTGAGGAACTAAACTTAATCCGTAATGTGTGCCTTCATCTACAAGATAGATTAGACGAAATCGAAGAGGAACATGATAATGAGAAAGCTGAGTGGGAAAGGGACAAAGAAGAGCTAACATTGCAACTAGTAACGCTGCAATCTCAGCTTGATGATATTCAGCATAGAATAAGGATGGCAAATGAATCTTTTTCAATGCCTCCCTTGGATGCACTTTCCATTAGGGATGACGAGGGTGACGATGCATTAGTTATATATACTTTGTAA

mRNA sequence

ATGTCTACTTCGGAAATTTTTGAAGAATCTGAACTAGAATGTTACTGTGGACTGCCTGCAAAACTGAGAACATCTCAGACACCCAAAAATCCATTCAGATTATTCTACAACTGTCCAAAGGAAATATCTCAACAATGTGGTTTTTTTCATTGGGCTGATGAACCCGAACCATCTGATGATAGACACGTTGAGGAACTAAACTTAATCCGTAATGTGTGCCTTCATCTACAAGATAGATTAGACGAAATCGAAGAGGAACATGATAATGAGAAAGCTGAGTGGGAAAGGGACAAAGAAGAGCTAACATTGCAACTAGTAACGCTGCAATCTCAGCTTGATGATATTCAGCATAGAATAAGGATGGCAAATGAATCTTTTTCAATGCCTCCCTTGGATGCACTTTCCATTAGGGATGACGAGGGTGACGATGCATTAGTTATATATACTTTGTAA

Coding sequence (CDS)

ATGTCTACTTCGGAAATTTTTGAAGAATCTGAACTAGAATGTTACTGTGGACTGCCTGCAAAACTGAGAACATCTCAGACACCCAAAAATCCATTCAGATTATTCTACAACTGTCCAAAGGAAATATCTCAACAATGTGGTTTTTTTCATTGGGCTGATGAACCCGAACCATCTGATGATAGACACGTTGAGGAACTAAACTTAATCCGTAATGTGTGCCTTCATCTACAAGATAGATTAGACGAAATCGAAGAGGAACATGATAATGAGAAAGCTGAGTGGGAAAGGGACAAAGAAGAGCTAACATTGCAACTAGTAACGCTGCAATCTCAGCTTGATGATATTCAGCATAGAATAAGGATGGCAAATGAATCTTTTTCAATGCCTCCCTTGGATGCACTTTCCATTAGGGATGACGAGGGTGACGATGCATTAGTTATATATACTTTGTAA

Protein sequence

MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDDRHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIRMANESFSMPPLDALSIRDDEGDDALVIYTL

Homology

[Close]

BLAST of Moc04g07330 vs. NCBI nr
Match: XP_022148840.1 (uncharacterized protein LOC111017399 [Momordica charantia])

HSP 1 Score: 310.5 bits (794), Expect = 8.1e-81
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD
Sbjct: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR
Sbjct: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120

Query: 121 MANESFSMPPLDALSIRDDEGDDALVIYTL 151
           MANESFSMPPLDALSIRDDEGDDALVIYTL
Sbjct: 121 MANESFSMPPLDALSIRDDEGDDALVIYTL 150

[Close]

BLAST of Moc04g07330 vs. NCBI nr
Match: XP_004136954.1 (uncharacterized protein LOC101219463 [Cucumis sativus] >XP_031745287.1 uncharacterized protein LOC101219463 [Cucumis sativus])

HSP 1 Score: 236.1 bits (601), Expect = 2.0e-58
Identity = 111/150 (74.00%), Postives = 130/150 (86.67%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MS+SEI+EES LECYCG+ AKLR S T K PFRLFYNCPKEISQQCGFFHWADE EPS+D
Sbjct: 1   MSSSEIYEESYLECYCGVAAKLRMSHTEKGPFRLFYNCPKEISQQCGFFHWADEREPSND 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RH  EL+LIRNVC+ L +RLDEI EEH++EK EWER+K ELTL+L TLQ+QLDDI +R+R
Sbjct: 61  RHANELDLIRNVCIRLTERLDEIVEEHEDEKEEWEREKAELTLKLSTLQTQLDDIHNRVR 120

Query: 121 MANESFSMPPLDALSIRDDEGDDALVIYTL 151
           + NESFSMPP ++LSIRDD+ D+ LVIYTL
Sbjct: 121 ITNESFSMPPFESLSIRDDDDDNTLVIYTL 150

[Close]

BLAST of Moc04g07330 vs. NCBI nr
Match: XP_008455003.1 (PREDICTED: uncharacterized protein LOC103495284 [Cucumis melo] >XP_008455004.1 PREDICTED: uncharacterized protein LOC103495284 [Cucumis melo] >XP_008455006.1 PREDICTED: uncharacterized protein LOC103495284 [Cucumis melo] >XP_016901682.1 PREDICTED: uncharacterized protein LOC103495284 [Cucumis melo] >KAA0031344.1 uncharacterized protein E6C27_scaffold139G00980 [Cucumis melo var. makuwa] >TYK06797.1 uncharacterized protein E5676_scaffold13G00990 [Cucumis melo var. makuwa])

HSP 1 Score: 235.7 bits (600), Expect = 2.5e-58
Identity = 112/151 (74.17%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MS+SEI+EES LECYCG  AKLR S T KNPFRLFYNCPKE+SQQCGFFHWADEPEPSDD
Sbjct: 1   MSSSEIYEESYLECYCGAAAKLRMSHTEKNPFRLFYNCPKELSQQCGFFHWADEPEPSDD 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RH  EL+LIRNVC+ L +RLDEI EEH++EK EWER+K ELTL+L TLQ+QLDDI +RIR
Sbjct: 61  RHANELDLIRNVCIRLTERLDEIIEEHEDEKEEWEREKAELTLKLSTLQTQLDDINNRIR 120

Query: 121 MANESFSMPPLDALSIR-DDEGDDALVIYTL 151
           + NESFSMPP ++LSIR DD+ +D LV+Y L
Sbjct: 121 ITNESFSMPPFESLSIRDDDDNNDTLVVYAL 151

[Close]

BLAST of Moc04g07330 vs. NCBI nr
Match: KAE8645848.1 (hypothetical protein Csa_017077 [Cucumis sativus])

HSP 1 Score: 226.9 bits (577), Expect = 1.2e-55
Identity = 106/147 (72.11%), Postives = 126/147 (85.71%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MS+SEI+EES LECYCG+ AKLR S T K PFRLFYNCPKEISQQCGFFHWADE EPS+D
Sbjct: 1   MSSSEIYEESYLECYCGVAAKLRMSHTEKGPFRLFYNCPKEISQQCGFFHWADEREPSND 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RH  EL+LIRNVC+ L +RLDEI EEH++EK EWER+K ELTL+L TLQ+QLDDI +R+R
Sbjct: 61  RHANELDLIRNVCIRLTERLDEIVEEHEDEKEEWEREKAELTLKLSTLQTQLDDIHNRVR 120

Query: 121 MANESFSMPPLDALSIRDDEGDDALVI 148
           + NESFSMPP ++LSIRDD+ D+ + I
Sbjct: 121 ITNESFSMPPFESLSIRDDDDDNTVTI 147

[Close]

BLAST of Moc04g07330 vs. NCBI nr
Match: KAF2294607.1 (hypothetical protein GH714_013386 [Hevea brasiliensis])

HSP 1 Score: 196.8 bits (499), Expect = 1.3e-46
Identity = 89/143 (62.24%), Postives = 113/143 (79.02%), Query Frame = 0


Query: 8   EESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDDRHVEELN 67
           E+SE+ C CGLPAKLR SQTP+NPFRLFYNCP+  + QCGFF W DEP P+ DRH +E+N
Sbjct: 25  EDSEVRCSCGLPAKLRVSQTPRNPFRLFYNCPRSCNFQCGFFRWWDEPAPTGDRHTDEVN 84

Query: 68  LIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIRMANESFS 127
           LIRN C  LQ RLDEIE+ H+N+++ WER+K EL L+L T+Q++LD+I+ R+R  NES  
Sbjct: 85  LIRNECARLQGRLDEIEQRHENDRSVWEREKSELMLKLSTVQTELDEIKERLRTVNESDL 144

Query: 128 MPPLDALSIRDDEGDDALVIYTL 151
           MPPLD L I DDEGDD + I+T+
Sbjct: 145 MPPLDNLLIADDEGDDVIEIHTI 167

[Close]

BLAST of Moc04g07330 vs. ExPASy TrEMBL
Match: A0A6J1D427 (uncharacterized protein LOC111017399 OS=Momordica charantia OX=3673 GN=LOC111017399 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 3.9e-81
Identity = 150/150 (100.00%), Postives = 150/150 (100.00%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD
Sbjct: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR
Sbjct: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120

Query: 121 MANESFSMPPLDALSIRDDEGDDALVIYTL 151
           MANESFSMPPLDALSIRDDEGDDALVIYTL
Sbjct: 121 MANESFSMPPLDALSIRDDEGDDALVIYTL 150

[Close]

BLAST of Moc04g07330 vs. ExPASy TrEMBL
Match: A0A0A0K2H6 (GRF-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071640 PE=4 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 9.5e-59
Identity = 111/150 (74.00%), Postives = 130/150 (86.67%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MS+SEI+EES LECYCG+ AKLR S T K PFRLFYNCPKEISQQCGFFHWADE EPS+D
Sbjct: 1   MSSSEIYEESYLECYCGVAAKLRMSHTEKGPFRLFYNCPKEISQQCGFFHWADEREPSND 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RH  EL+LIRNVC+ L +RLDEI EEH++EK EWER+K ELTL+L TLQ+QLDDI +R+R
Sbjct: 61  RHANELDLIRNVCIRLTERLDEIVEEHEDEKEEWEREKAELTLKLSTLQTQLDDIHNRVR 120

Query: 121 MANESFSMPPLDALSIRDDEGDDALVIYTL 151
           + NESFSMPP ++LSIRDD+ D+ LVIYTL
Sbjct: 121 ITNESFSMPPFESLSIRDDDDDNTLVIYTL 150

[Close]

BLAST of Moc04g07330 vs. ExPASy TrEMBL
Match: A0A1S4E0B8 (uncharacterized protein LOC103495284 OS=Cucumis melo OX=3656 GN=LOC103495284 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.2e-58
Identity = 112/151 (74.17%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MS+SEI+EES LECYCG  AKLR S T KNPFRLFYNCPKE+SQQCGFFHWADEPEPSDD
Sbjct: 1   MSSSEIYEESYLECYCGAAAKLRMSHTEKNPFRLFYNCPKELSQQCGFFHWADEPEPSDD 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RH  EL+LIRNVC+ L +RLDEI EEH++EK EWER+K ELTL+L TLQ+QLDDI +RIR
Sbjct: 61  RHANELDLIRNVCIRLTERLDEIIEEHEDEKEEWEREKAELTLKLSTLQTQLDDINNRIR 120

Query: 121 MANESFSMPPLDALSIR-DDEGDDALVIYTL 151
           + NESFSMPP ++LSIR DD+ +D LV+Y L
Sbjct: 121 ITNESFSMPPFESLSIRDDDDNNDTLVVYAL 151

[Close]

BLAST of Moc04g07330 vs. ExPASy TrEMBL
Match: A0A5A7SJM5 (GRF-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00990 PE=4 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 1.2e-58
Identity = 112/151 (74.17%), Postives = 130/151 (86.09%), Query Frame = 0


Query: 1   MSTSEIFEESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDD 60
           MS+SEI+EES LECYCG  AKLR S T KNPFRLFYNCPKE+SQQCGFFHWADEPEPSDD
Sbjct: 1   MSSSEIYEESYLECYCGAAAKLRMSHTEKNPFRLFYNCPKELSQQCGFFHWADEPEPSDD 60

Query: 61  RHVEELNLIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIR 120
           RH  EL+LIRNVC+ L +RLDEI EEH++EK EWER+K ELTL+L TLQ+QLDDI +RIR
Sbjct: 61  RHANELDLIRNVCIRLTERLDEIIEEHEDEKEEWEREKAELTLKLSTLQTQLDDINNRIR 120

Query: 121 MANESFSMPPLDALSIR-DDEGDDALVIYTL 151
           + NESFSMPP ++LSIR DD+ +D LV+Y L
Sbjct: 121 ITNESFSMPPFESLSIRDDDDNNDTLVVYAL 151

[Close]

BLAST of Moc04g07330 vs. ExPASy TrEMBL
Match: A0A6A6L2I8 (GRF-type domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_013230 PE=4 SV=1)

HSP 1 Score: 196.8 bits (499), Expect = 6.4e-47
Identity = 89/143 (62.24%), Postives = 113/143 (79.02%), Query Frame = 0


Query: 8   EESELECYCGLPAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADEPEPSDDRHVEELN 67
           E+SE+ C CGLPAKLR SQTP+NPFRLFYNCP+  + QCGFF W DEP P+ DRH +E+N
Sbjct: 33  EDSEVRCSCGLPAKLRVSQTPRNPFRLFYNCPRSCNFQCGFFRWWDEPAPTGDRHTDEVN 92

Query: 68  LIRNVCLHLQDRLDEIEEEHDNEKAEWERDKEELTLQLVTLQSQLDDIQHRIRMANESFS 127
           LIRN C  LQ RLDEIE+ H+N+++ WER+K EL L+L T+Q++LD+I+ R+R  NES  
Sbjct: 93  LIRNECARLQGRLDEIEQRHENDRSVWEREKSELMLKLSTVQTELDEIKERLRTVNESDL 152

Query: 128 MPPLDALSIRDDEGDDALVIYTL 151
           MPPLD L I DDEGDD + I+T+
Sbjct: 153 MPPLDNLLIADDEGDDVIEIHTI 175

[Close]

BLAST of Moc04g07330 vs. TAIR 10
Match: AT3G42860.1 (zinc knuckle (CCHC-type) family protein )

HSP 1 Score: 49.3 bits (116), Expect = 3.2e-06
Identity = 24/50 (48.00%), Postives = 29/50 (58.00%), Query Frame = 0


Query: 11  ELECYCGLP-AKLRTSQTPKNPFRLFYNCP-KEISQQCGFFHWADEPEPS 59
           E  C CG+    + TS TPKNP R FY CP +E +  CGFF W D  + S
Sbjct: 93  EKSCPCGVGICLILTSNTPKNPGRKFYKCPNREENGGCGFFQWCDAVQSS 142

[Close]

BLAST of Moc04g07330 vs. TAIR 10
Match: AT1G54930.1 (GRF zinc finger / Zinc knuckle protein )

HSP 1 Score: 42.7 bits (99), Expect = 3.0e-04
Identity = 19/45 (42.22%), Postives = 24/45 (53.33%), Query Frame = 0


Query: 11 ELECYCGL-PAKLRTSQTPKNPFRLFYNCPKEISQQCGFFHWADE 55
          ++ C C   P    TS+T KNP R FY CP      CG+F W D+
Sbjct: 37 DIHCPCNAGPCNTVTSKTEKNPNRRFYTCP-----SCGYFKWCDQ 76

The following BLAST results are available for this feature:

BLAST of Moc04g07330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148840.1	8.1e-81	100.00	uncharacterized protein LOC111017399 [Momordica charantia]	[more]
XP_004136954.1	2.0e-58	74.00	uncharacterized protein LOC101219463 [Cucumis sativus] >XP_031745287.1 uncharact...	[more]
XP_008455003.1	2.5e-58	74.17	PREDICTED: uncharacterized protein LOC103495284 [Cucumis melo] >XP_008455004.1 P...	[more]
KAE8645848.1	1.2e-55	72.11	hypothetical protein Csa_017077 [Cucumis sativus]	[more]
KAF2294607.1	1.3e-46	62.24	hypothetical protein GH714_013386 [Hevea brasiliensis]	[more]

BLAST of Moc04g07330 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc04g07330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1D427	3.9e-81	100.00	uncharacterized protein LOC111017399 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A0A0K2H6	9.5e-59	74.00	GRF-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G071640 PE...	[more]
A0A1S4E0B8	1.2e-58	74.17	uncharacterized protein LOC103495284 OS=Cucumis melo OX=3656 GN=LOC103495284 PE=...	[more]
A0A5A7SJM5	1.2e-58	74.17	GRF-type domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6A6L2I8	6.4e-47	62.24	GRF-type domain-containing protein OS=Hevea brasiliensis OX=3981 GN=GH714_013230...	[more]

BLAST of Moc04g07330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G42860.1	3.2e-06	48.00	zinc knuckle (CCHC-type) family protein	[more]
AT1G54930.1	3.0e-04	42.22	GRF zinc finger / Zinc knuckle protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 66..125
None	No IPR available	PANTHER	PTHR33248	ZINC ION-BINDING PROTEIN	coord: 5..139
None	No IPR available	PANTHER	PTHR33248:SF19	RETICULOCYTE-BINDING PROTEIN 2 HOMOLOG A-LIKE	coord: 5..139
IPR010666	Zinc finger, GRF-type	PFAM	PF06839	zf-GRF	coord: 14..54 e-value: 1.4E-13 score: 50.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g07330.1	Moc04g07330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0008270	zinc ion binding