Moc04g07190 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g07190
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionLipase
Locationchr4: 5095057 .. 5100728 (-)
RNA-Seq ExpressionMoc04g07190
SyntenyMoc04g07190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAGCCATTGCTTGCTCTGATGTTTCTTCTCTACTTCGCTCTTCACGCCACCGATGATTCTTCCGATCGTTCCACCGAGCATTCGATTCTCCATCGCGCTTCGTCGGAGCAGAGGACCTTGTGCTCTCAACTCGTCGAACCTGCTGGCTATCCTTGCGAGGAGCATAAGGTACTGTGATTCTCTGTTCTCGCTGCTGATTATTAGGAATTGTTTGTGAAATGAGACAGTATTAGTGATCGCTCTGTTCGTAGTGGAGTAACTGGAAAGGATTTAGTCAACTAATTTGCTGCAATGCTTCGTGTTCGAGAGTTATTTCTTGTCCGTGATTGAATGATTTGATTTGTTTATCTGTCGAGTCTGGACAAACTGATTCATTTATATCGATCTTTGTGTTCATTGTGTTTATTAGCAATTCTGAGCTCGACGCATTTGGTATACTTGAGCACTCTTTATATGGTTGTGCAGTGCTGACGGACATGATTTTCTCATAAATTTCTTAGATTCAAACGAAGGATGGCTTTTTGTTGGGTCTTCAACGTGTATCTTCATACGATGGAGATTTGAAAAAAATAAAAGGCCCTCCAATTTTGCTCCTCCATGGACTTCTCATGGTGAGCCTTCTTACAGTTTTAAAATATTGTAACTGTATTTGTCTAATCAATTTACTCTTTGTTTTATCAGCAGTAGCTAGAAACTCGACAAGGTTTCCTGAGTTTTGCATTCTTTATTTTATTTTTAATTTCTCATTAGCAATATCAAACTCCTGTTTTTAACAAAAGGATTGACGACATCTGATATTTGTTATACTATTGAGGCTTTTTTTTGTATCTATTTTCATTGTAATTTCAACTGCACCAATCTCTTGATATTGCAGTAAAATGATTATTTTCCCGGAAAAAGTTATGTGAGGGTTCTGTATTAGGTCAGTACTAACCTTCTTGACTACTACAGTCCGGTCATTGTCACTTCAAAAATTGAAGACCTCAAGTTTGCAAGCGAATGTTATTTAAAGGAGATTATAATTTGGAGTATAATTGCTGTGCTGACAATGCGCTAAATATGAGTTAATCTTAACGAATTCTTGATTAGCTCAAAGTCTGGCTGATGCTGAAATAATTGCGGAAGTGGTTAGTAAAATATTGAAGAGAGGAGGGAGAAATAACTAATTGCAAAGGGGGAATCAGAGGAATGTAGGGAAGGGTGAGACTGGTGAGCTTTGAGTTCTTTATGGATTTTCATTAAAGTTGTGGGAGAGCTAAGGGACTCTTTGAATTTCTCCCATTTTTCCTTTCCTAAATAGAAGTAATTAGAGCTTTCAGCTCTATCAACTACCTAGCCGGTTTAACTATTATGCAATCCATCACTGCTGGTAATTATTATGCAATCCATCACTGCTGGAAGCATGTGATGAACACTCACAAATTTAGAATTAACAAGACTTGACTGAGAATAAACAACTTTACTTGTATTGAAATTTCTCACAAAATACACAATTGGTTTACCAATCTCTCTTGTTTTCTTCTCCATACACTTGTTTGAAATACATCAAAACATCATCCTATTTATAGGACTCCATGGAAGAATCTAGATCCATAAATTCTCATCTTATGAACCTATACATACACGAACATATGATTAATTCATCTTAAAAAACTAAATTCATGAACATAAAATTAATTACTAGTTCATGTTAATGTATCATGTACTTAGGGATGTACATGTTTAATTTTCAACAATCACGCACACTGTGTATATGTCTGTTTATATGTTTATGTTGTTCCTTTAATCAAGCCTTCTATTTTCTTCTCCTTCCAAGTGAGATTCTTTTCAAATTTTTGCTACCCATCTGAAACAATCTGACAAATCTAACTATCCTTAAAATTTTCTTGGGTCCATGACATCAATAGCTGGATCTTGAAACATGAATTGTAAAATTTGAATTATTTCGTGGACTGGAATCATGCTCATAGTTGAGGGACGTGGTTCCTACTACTAGCTATTTCAAACCTTTCATTGTCTAAGTTGGTGCAAATGCAGGCAGGCGATGCATGGTTTTTGAATTCTGCGGAACAATCTTTAGGCTTCATCCTTGCTGAAAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTCATGGGCATATATCGCTATCGGAGCATGATAAGGTATTAATAGATTAGTTTATTATAAAATGCCTAAGATGGATAGGGTGTTAATTAGATGAAAAGCGAACATATTAATAGAAAGGGTTTCTCCAGTGTGTTGTGGGTGCATTGCATGTATCTAGAAAATTTAATAATGATGCAAATATGCTGCTGCTCATATATGCTCAATGCATTGAGGTAGAGAGTATCATGCAGTTGATCGTAAAGGTTCAAACAAATAGAGGTGAGGTTTTATGGAGTTAAGCCGTTACATGACAGTTTATCTTAAATAGACCATTCGTTACATGCTATTTGTGTCCGTTTACTAAAGCCTTTTCTGCCTCAAGTCTCTCCACTATGTTGTCTGTTGGATTACTGGTTCATAAGTATCTTATGTCAATCTTAAAAGTAATGTTAACATAAATTGCATTTGTTAAGTTTCTGTTTATTGGTGTTTGATTCTAGTATATTCTTATTGTAGAAATTTTGGAATTGGAGTTGGGAAGATTTGGCTCTGTATGATCTCGCAGAAATGATTGACTACATAAATTCATTAACAGACAGGAAAGTTTATGTTGTTGGGCATTCACAGGTCCCATATCTCTTCTCATACAGGATTCTTCGTCTGAAAATTTTCCTTCTTGCTCTTGTGTCATCTCCTGTCTTGTTTGCAGGGAACAATCATATCTTTTGTTGCTCTTACCCAACCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTCTTAGTCCTATATCGTACTTGGACCATATCACTGCTCCATTAGTACGTCTAATGGTTGACTTGCATCTTGATACGGTATTCCTTGCTTGTTATTGTTTGATTAGCATTGTTAAATGATCGTACAGATTTTAGTAATAAGCTTATTCTCTGTAGTTTTTTTTCGGCAGATGATTCTTACAATGGGATTTCATCAGCTTAACTTTAAAAGGTTGTCATTCCATTATCCAATTTGATTTGTAATTGGTTGCATTTTTAGCTTCAGTGTTGTTTGTAAAACTGATGCCGATGTTTCTTTACACTACTTCAGTGATTGGGGAACAAACCTTTTAGATATGCTTTGTGATCAGCTCGTTGACTGCAACAATATTCTAAGTTCAATAACAGGTTATCTCCACAACTCGCTTCTTTTCCCCCACCCCTTCTTACTTTTTTTCTTTACCTTCCAAGCCATTGTGCATAGGAAATTGTTGCCAGAATGCTTTCTTCTAGTTCTTTCTTGTTTTGTTTTTTTCTCTCTTTTGCAATCTTAGAATTTGTGATTCAATATTATTAATTTCTGTTGTCTCCATCCCAGTTGGCAAGGGCGTGGAATCTCTTAGTCATACTGATTTAAAAGTCTCAATAATACCAAAAATTCTTAATGTCTTCCAGGTCCGGGTCAACCCTGATTATAGGGGAGCAAAGCTCCGACTCCTTATTATTAAAAAAAAAAAAGTTGCTAATCTCTGTCAATTACTCTGATGCATGTTGACATTGATCATTATTAACCATGCACGCTTACATTGATCATTATTAACTGTATATGTTCCTCTTTATATTCTGGCATTGATCATTATTAAGTCCTATTTTTTGGATAGGAGGATTATTAAGTACCAAGTTCTCTCATGAGTACTAAGTTCTCCGCGAAACCTTTTAATTTTATCATTTTATGACTTGTAAATCAATCTGTTACTCTTTTGTCTTGGAAATACAGCGTTGAACATAGATGAGAGAAAATAAATAAATATATTCACATGACAATTGGCATAGAGCTATAAGAAATTATACTGAGTTTTCAATACTCTTGTGGTATCAGCCATGTTCTCCTCTGACATGTGCTTGGCGGGAAAGTGGTTGGGAGCAGACATTCTGTAGTTTAGGTGGAGTTTTTTGTCGTGCCTATAAATCTTCATGTAATGTTGTTCATAGCAAATAGGTTCCAACAAAAATAAGACAACAAATTATACAGCCTGATAAAATCTTACTGTATCTGCACTGTAAGTCGATTACTCCATGTATTTCCCACCAAAGTCTGTATCATAGTTTTCACGATATCTTGCTGAAGCCTGCCTAAAGATAAGATCTCATTTCACTAGACTCTAGCTACACAGGTAATTTAAAGTATTCTGTGCCTAGTGACAATCGAACGGACCCTAACATGAAGATGGATGGCGTTTATATATTTATAATGTCATGTTTCTTGTTTTCAGAAGAAAATTGGGTAGAACTAGAAAAAGAGGATCGATAATGAATTGTTGTTATTGGAATGATGGTCTGAAATATTGCTTTATCTTTACAGGGAAGTATCAATTTTGAATGTTCAATCTATTCAAATACTCAGAAACTTGAATTTCCTAACAAGGAATTCTTATAATGCTGCAGGGAAGAACTGTTACCTCAATGGCTCACGTTTTGACTTCTTTTTTGAATTCGAACCACATCCAACATCTGCAAAAAACCTACACCATCTTTTCCAGAGTACGCTCATGATCTTCTCTCAGCTTTATGTTCTATTATACTATGACTGATGAATCTATAAATTCTTTACCTGCATTTTATGCATAGTCTAATACAAATCCTTGAACATCCAATTGTGTAACTTGGGCGTCATACATTTTTATTCTAGCAAGTGACTTTCCTTTCTCGCAAAGGAAACCAACTTTTAAGAAGTTACCAAGTCCAAAACTCCTTTAGGAGCATACAAAAATCACCCCGTTTATCAAGAAAATAGTAAAATTATGAGAACAACCCTTGGTAAACTACAAAGAAAACATCCCCACTAGAAAAGAACTCGAAAATTGATCTTCTTCAAAACTTTTCCAAGAAACCTCCTAGAGATGCTAAAAAGCCCATCAAGTTTTCTTCAACAACCTCTTTCACAGGAAAAATGCCAAGTTTTCCTCTTCCATTGTTGCACTTCAAAACGATTACCTAAGCTCCCCATTAATGCTAAAGTTCCTCTTCAACTAAAGTCTATTTACTACTTATATTACTACTGCAGCCATCAGAACTGTAGCCGAAAGTATCCCCAAAATCACACCGCTGTGATTTCCAGTTGAATCTAAAACCTCTTTACATAGAAATTGCATGCCAATAATATCTTTGGAAATATATGACCACTTCACTAAATTCTCCCGGGAAACTGTTCAAGCCAGGCTGGTTTAACCGCTTTCTTTTTCTCCAGTGATCCGCAAGGGTACTTTTTCCAGGTATGACTATGGATTGTTAAACAATCTGAGAGTGTATGGTCGTTCAAAGCCTCCTGCATTCGATCTCCGCCGTATTCCAAAGTCGTTGCCGCTGTGGATGGCGTATGGTGGCAATGACGAGTTGTCAGATTGGACAGATCTGCAGCACACCATCAAGGAGTTGGATTCGGTACCAGAATTGGTTTACCTTGAGAATTATGGTCATGTAGACTTCATTTTGAGTGTGAGAGCCAAGGAAGATGTGTATGATCCTATGATCAAATTCTTTAAATCCTTGGGGAAGTCCAGTAGTTTGTAA

mRNA sequence

ATGGCGAAGCCATTGCTTGCTCTGATGTTTCTTCTCTACTTCGCTCTTCACGCCACCGATGATTCTTCCGATCGTTCCACCGAGCATTCGATTCTCCATCGCGCTTCGTCGGAGCAGAGGACCTTGTGCTCTCAACTCGTCGAACCTGCTGGCTATCCTTGCGAGGAGCATAAGATTCAAACGAAGGATGGCTTTTTGTTGGGTCTTCAACGTGTATCTTCATACGATGGAGATTTGAAAAAAATAAAAGGCCCTCCAATTTTGCTCCTCCATGGACTTCTCATGGCAGGCGATGCATGGTTTTTGAATTCTGCGGAACAATCTTTAGGCTTCATCCTTGCTGAAAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTCATGGGCATATATCGCTATCGGAGCATGATAAGAAATTTTGGAATTGGAGTTGGGAAGATTTGGCTCTGTATGATCTCGCAGAAATGATTGACTACATAAATTCATTAACAGACAGGAAAGTTTATGTTGTTGGGCATTCACAGGGAACAATCATATCTTTTGTTGCTCTTACCCAACCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTCTTAGTCCTATATCGTACTTGGACCATATCACTGCTCCATTAGTACGTCTAATGGTTGACTTGCATCTTGATACGATGATTCTTACAATGGGATTTCATCAGCTTAACTTTAAAAGTGATTGGGGAACAAACCTTTTAGATATGCTTTGTGATCAGCTCGTTGACTGCAACAATATTCTAAGTTCAATAACAGGGAAGAACTGTTACCTCAATGGCTCACGTTTTGACTTCTTTTTTGAATTCGAACCACATCCAACATCTGCAAAAAACCTACACCATCTTTTCCAGATGATCCGCAAGGGTACTTTTTCCAGGTATGACTATGGATTGTTAAACAATCTGAGAGTGTATGGTCGTTCAAAGCCTCCTGCATTCGATCTCCGCCGTATTCCAAAGTCGTTGCCGCTGTGGATGGCGTATGGTGGCAATGACGAGTTGTCAGATTGGACAGATCTGCAGCACACCATCAAGGAGTTGGATTCGGTACCAGAATTGGTTTACCTTGAGAATTATGGTCATGTAGACTTCATTTTGAGTGTGAGAGCCAAGGAAGATGTGTATGATCCTATGATCAAATTCTTTAAATCCTTGGGGAAGTCCAGTAGTTTGTAA

Coding sequence (CDS)

ATGGCGAAGCCATTGCTTGCTCTGATGTTTCTTCTCTACTTCGCTCTTCACGCCACCGATGATTCTTCCGATCGTTCCACCGAGCATTCGATTCTCCATCGCGCTTCGTCGGAGCAGAGGACCTTGTGCTCTCAACTCGTCGAACCTGCTGGCTATCCTTGCGAGGAGCATAAGATTCAAACGAAGGATGGCTTTTTGTTGGGTCTTCAACGTGTATCTTCATACGATGGAGATTTGAAAAAAATAAAAGGCCCTCCAATTTTGCTCCTCCATGGACTTCTCATGGCAGGCGATGCATGGTTTTTGAATTCTGCGGAACAATCTTTAGGCTTCATCCTTGCTGAAAATGGATTTGATGTGTGGATAGGAAATGTGCGTGGAACACGGTGGAGTCATGGGCATATATCGCTATCGGAGCATGATAAGAAATTTTGGAATTGGAGTTGGGAAGATTTGGCTCTGTATGATCTCGCAGAAATGATTGACTACATAAATTCATTAACAGACAGGAAAGTTTATGTTGTTGGGCATTCACAGGGAACAATCATATCTTTTGTTGCTCTTACCCAACCAGACATAGCCAAGAAAGTTGAAGCAGCTGCTCTTCTTAGTCCTATATCGTACTTGGACCATATCACTGCTCCATTAGTACGTCTAATGGTTGACTTGCATCTTGATACGATGATTCTTACAATGGGATTTCATCAGCTTAACTTTAAAAGTGATTGGGGAACAAACCTTTTAGATATGCTTTGTGATCAGCTCGTTGACTGCAACAATATTCTAAGTTCAATAACAGGGAAGAACTGTTACCTCAATGGCTCACGTTTTGACTTCTTTTTTGAATTCGAACCACATCCAACATCTGCAAAAAACCTACACCATCTTTTCCAGATGATCCGCAAGGGTACTTTTTCCAGGTATGACTATGGATTGTTAAACAATCTGAGAGTGTATGGTCGTTCAAAGCCTCCTGCATTCGATCTCCGCCGTATTCCAAAGTCGTTGCCGCTGTGGATGGCGTATGGTGGCAATGACGAGTTGTCAGATTGGACAGATCTGCAGCACACCATCAAGGAGTTGGATTCGGTACCAGAATTGGTTTACCTTGAGAATTATGGTCATGTAGACTTCATTTTGAGTGTGAGAGCCAAGGAAGATGTGTATGATCCTATGATCAAATTCTTTAAATCCTTGGGGAAGTCCAGTAGTTTGTAA

Protein sequence

MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL
Homology
BLAST of Moc04g07190 vs. NCBI nr
Match: XP_022148780.1 (triacylglycerol lipase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 835.5 bits (2157), Expect = 2.0e-238
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ
Sbjct: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
           TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV
Sbjct: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240
           TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK
Sbjct: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240

Query: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300
           SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI
Sbjct: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300

Query: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360
           RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE
Sbjct: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360

Query: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 405

BLAST of Moc04g07190 vs. NCBI nr
Match: XP_022148777.1 (triacylglycerol lipase 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 782.7 bits (2020), Expect = 1.5e-222
Identity = 377/405 (93.09%), Postives = 392/405 (96.79%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDS DRS +HSIL R SSEQ+TLCSQLVEPAGYPCEEHKIQ
Sbjct: 1   MAKPLLALMFLLYFALHATDDSYDRSADHSILPRGSSEQKTLCSQLVEPAGYPCEEHKIQ 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
           TKDGFLLGLQRVSSYDGDLK+I+GPPILLLHGLLMAGDAWFLNSA+QSLGFILA+NGFDV
Sbjct: 61  TKDGFLLGLQRVSSYDGDLKEIQGPPILLLHGLLMAGDAWFLNSADQSLGFILADNGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240
           TI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPLVRLMVDLHLDTMIL MGFH+LNFK
Sbjct: 181 TIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPLVRLMVDLHLDTMILAMGFHELNFK 240

Query: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300
           SDWGT+LLDMLCDQLV+C +ILS ITGKNCYLNGSRFD FFE+EPHPTSAKNL HLFQMI
Sbjct: 241 SDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGSRFDIFFEYEPHPTSAKNLRHLFQMI 300

Query: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360
           RKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE
Sbjct: 301 RKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360

Query: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF KSLGK SSL
Sbjct: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFIKSLGKFSSL 405

BLAST of Moc04g07190 vs. NCBI nr
Match: XP_022148776.1 (triacylglycerol lipase 1-like isoform X1 [Momordica charantia])

HSP 1 Score: 778.1 bits (2008), Expect = 3.7e-221
Identity = 377/406 (92.86%), Postives = 392/406 (96.55%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDS DRS +HSIL R SSEQ+TLCSQLVEPAGYPCEEHKIQ
Sbjct: 1   MAKPLLALMFLLYFALHATDDSYDRSADHSILPRGSSEQKTLCSQLVEPAGYPCEEHKIQ 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
           TKDGFLLGLQRVSSYDGDLK+I+GPPILLLHGLLMAGDAWFLNSA+QSLGFILA+NGFDV
Sbjct: 61  TKDGFLLGLQRVSSYDGDLKEIQGPPILLLHGLLMAGDAWFLNSADQSLGFILADNGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDT-MILTMGFHQLNF 240
           TI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPLVRLMVDLHLDT MIL MGFH+LNF
Sbjct: 181 TIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPLVRLMVDLHLDTQMILAMGFHELNF 240

Query: 241 KSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQM 300
           KSDWGT+LLDMLCDQLV+C +ILS ITGKNCYLNGSRFD FFE+EPHPTSAKNL HLFQM
Sbjct: 241 KSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGSRFDIFFEYEPHPTSAKNLRHLFQM 300

Query: 301 IRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK 360
           IRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK
Sbjct: 301 IRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK 360

Query: 361 ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF KSLGK SSL
Sbjct: 361 ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFIKSLGKFSSL 406

BLAST of Moc04g07190 vs. NCBI nr
Match: XP_022148781.1 (triacylglycerol lipase 1-like isoform X2 [Momordica charantia])

HSP 1 Score: 742.3 bits (1915), Expect = 2.3e-210
Identity = 368/405 (90.86%), Postives = 368/405 (90.86%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHK  
Sbjct: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHK-- 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
                                              AGDAWFLNSAEQSLGFILAENGFDV
Sbjct: 61  -----------------------------------AGDAWFLNSAEQSLGFILAENGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240
           TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK
Sbjct: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240

Query: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300
           SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI
Sbjct: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300

Query: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360
           RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE
Sbjct: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360

Query: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 368

BLAST of Moc04g07190 vs. NCBI nr
Match: XP_022952782.1 (triacylglycerol lipase 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 706.4 bits (1822), Expect = 1.4e-199
Identity = 339/406 (83.50%), Postives = 374/406 (92.12%), Query Frame = 0

Query: 1   MAKPLLALMFLL-YFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKI 60
           MAKPL+A++FLL +FALH+ DDSS RS E+SIL R  S+Q++LCSQLV+PAGYPC EH+I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFD 120
           QTKDGFLLGLQRVSS DGDL+K KGPPILLLHGL M GDAWFLNSA +SLGFILA+NGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSE +K+FW+WSWE+LALYDLA MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNF 240
           GTI+SF ALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+IL  GFH+LNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQM 300
           KSDWGT LLD LCD+LV+C N+LSSITG+NC LNGSRFD FFE+EPHP+SAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK 360
           IRKGTFSRYDYGLL NLRVYG+ KPPAFDL RIPKSLPLWMAYGG+DELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           EL SVPELVYLENYGHVDFILS++AKEDVYDPMIKF KSLGKS +L
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406

BLAST of Moc04g07190 vs. ExPASy Swiss-Prot
Match: Q71DJ5 (Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 5.2e-141
Identity = 229/378 (60.58%), Postives = 297/378 (78.57%), Query Frame = 0

Query: 27  TEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKIKGPP 86
           T+  +LH   S   +LC+ L+ PA Y C EH IQTKDG++L LQRV+S    L+   GPP
Sbjct: 19  TQSHLLH--GSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQ--SGPP 78

Query: 87  ILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWN 146
           +LL HGL MAGD WFLNS ++SLGFILA++GFDVW+GNVRGTR+S+GH++LS+ DK+FW+
Sbjct: 79  VLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWD 138

Query: 147 WSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPI 206
           WSW+DLA+YDLAEMI Y+ S+++ K+++VGHSQGTI+SF ALTQP +A+ VEAAALL PI
Sbjct: 139 WSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPI 198

Query: 207 SYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSIT 266
           SYLDH+TAPLV  MV +HLD M++ +G HQ+NF+SD    L+D LC+  +DC + L+SIT
Sbjct: 199 SYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSIT 258

Query: 267 GKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPA 326
           G NC  N S+ +++ ++EPHP+S KN+ HLFQMIRKGTF++YDYG   NLR YG SKPP 
Sbjct: 259 GTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPE 318

Query: 327 FDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKE 386
           F L  IP SLP+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   AKE
Sbjct: 319 FILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKE 378

Query: 387 DVYDPMIKFFKSLGKSSS 405
           DVY  MI+FF++  KSSS
Sbjct: 379 DVYKHMIQFFRAKVKSSS 392

BLAST of Moc04g07190 vs. ExPASy Swiss-Prot
Match: Q67ZU1 (Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1)

HSP 1 Score: 289.7 bits (740), Expect = 5.3e-77
Identity = 155/408 (37.99%), Postives = 242/408 (59.31%), Query Frame = 0

Query: 6   LALMFLLYFALHATDDSSDRSTEHSILHRASSEQRT----LCSQLVEPAGYPCEEHKIQT 65
           LAL  L++FAL +      R T   +       QRT    +C+  V   GY CEEH + T
Sbjct: 14  LALSVLIFFAL-SLKTLEARGTFGRL--AGQPPQRTAAGGICASSVHIFGYKCEEHDVVT 73

Query: 66  KDGFLLGLQRVSSYDGDLKKIKG-----PPILLLHGLLMAGDAWFLNSAEQSLGFILAEN 125
           +DG++L +QR+   +G    + G      P+L+ HG+L+ G +W LN A+Q+L  ILA+ 
Sbjct: 74  QDGYILNMQRIP--EGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPADQNLPLILADQ 133

Query: 126 GFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVG 185
           GFDVW+GN RGTR+S  H  L+   + FWNW+W++L  YDL  M D+I+ LT +K++ +G
Sbjct: 134 GFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPAMFDHIHGLTGQKIHYLG 193

Query: 186 HSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQ 245
           HS GT+I F + ++  +  +V +AA+LSP++YL H+T  +  +     L      +G+ +
Sbjct: 194 HSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPE 253

Query: 246 LNFKSDWGTNLLDMLCDQL-VDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHH 305
            N KS    + +  +C +  +DC +++S ITGKNC LN S  D F   EP  TS KN+ H
Sbjct: 254 FNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIH 313

Query: 306 LFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDL 365
           L Q +R     +Y+YG  + N++ YG++ PPA+++  IP  LPL+ +YGG D L+D  D+
Sbjct: 314 LAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDV 373

Query: 366 -----QHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFK 398
                Q    ++D +  + ++++Y H DFI+ V AK+ VY+ +  FFK
Sbjct: 374 EFLLDQFKYHDIDKM-NVQFVKDYAHADFIMGVTAKDVVYNQVATFFK 415

BLAST of Moc04g07190 vs. ExPASy Swiss-Prot
Match: P04634 (Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.1e-50
Identity = 119/365 (32.60%), Postives = 198/365 (54.25%), Query Frame = 0

Query: 44  SQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKI-KGPPILLLHGLLMAGDAWFL 103
           SQ++   GYPC+E+++ T+DG++LG+ R+     + + I K P + L HGL+ +   W  
Sbjct: 35  SQMITYWGYPCQEYEVVTEDGYILGVYRIPHGKNNSENIGKRPVVYLQHGLIASATNWIA 94

Query: 104 NSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMID 163
           N    SL F+LA+ G+DVW+GN RG  WS  ++  S    +FW +S++++A YDL   I+
Sbjct: 95  NLPNNSLAFMLADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATIN 154

Query: 164 YINSLT-DRKVYVVGHSQGTIISFVAL-TQPDIAKKVEAAALLSPISYLDHITAPLVRLM 223
           +I   T   K++ VGHSQGT I F+A  T P +AKK++    L+P++ + +  +PL ++ 
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPTLAKKIKTFYALAPVATVKYTQSPLKKIS 214

Query: 224 VDLHLDTMILTMGFHQ------LNFKSDWGT-----NLLDMLCDQLVDCNNILSSITGKN 283
               + T +  + F +        F    GT      +LD+LC   +    I      KN
Sbjct: 215 ---FIPTFLFKLMFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTL---FIFCGFDKKN 274

Query: 284 CYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGL-LNNLRVYGRSKPPAFD 343
             LN SRFD +    P  TS ++  H  Q++R G F  +++G    N+  Y +  PP +D
Sbjct: 275 --LNVSRFDVYLGHNPAGTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYD 334

Query: 344 LRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKEDV 394
           +  +   + +W   GGND L+D  D+   + +L ++     +  Y H+DFI ++ A ++V
Sbjct: 335 VSAMTVPVAVW--NGGNDILADPQDVAMLLPKLSNLLFHKEILAYNHLDFIWAMDAPQEV 389

BLAST of Moc04g07190 vs. ExPASy Swiss-Prot
Match: Q9CPP7 (Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 2.1e-49
Identity = 123/365 (33.70%), Postives = 197/365 (53.97%), Query Frame = 0

Query: 44  SQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKI-KGPPILLLHGLLMAGDAWFL 103
           SQ++   GYP EE+++ T+DG++LG+ R+     + + I K P   L HGL+ +   W  
Sbjct: 35  SQMITYWGYPSEEYEVVTEDGYILGVYRIPYGKKNSENIGKRPVAYLQHGLIASATNWIT 94

Query: 104 NSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMID 163
           N    SL FILA+ G+DVW+GN RG  WS  ++  S    +FW +S++++A YDL   ID
Sbjct: 95  NLPNNSLAFILADAGYDVWLGNSRGNTWSRKNVYYSPDSVEFWAFSFDEMAKYDLPATID 154

Query: 164 YINSLT-DRKVYVVGHSQGTIISFVAL-TQPDIAKKVEAAALLSPISYLDHITAPL--VR 223
           +I   T   K++ VGHSQGT I F+A  T P +AKK++    L+P++ + +  +P   + 
Sbjct: 155 FIVQKTGQEKIHYVGHSQGTTIGFIAFSTNPALAKKIKRFYALAPVATVKYTESPFKKIS 214

Query: 224 LMVDLHLDTMILTMGFHQLNFKSDW-GT-----NLLDMLCDQLVDCNNILSSITGKNCYL 283
           L+    L  +     F   N+   + GT      LLD+LC   +    I      KN  L
Sbjct: 215 LIPKFLLKVIFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNAL---FIFCGFDKKN--L 274

Query: 284 NGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGL-LNNLRVYGRSKPPAFDLRR 343
           N SRFD +    P  TS ++L H  Q+ + G    Y++G  L N+  Y +  PP +D+  
Sbjct: 275 NVSRFDVYLGHNPAGTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSA 334

Query: 344 IPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLEN---YGHVDFILSVRAKEDV 394
           +   + +W   GG+D L+D  D+   + +L   P L+Y +    Y H+DFI ++ A ++V
Sbjct: 335 MTVPIAVW--NGGHDILADPQDVAMLLPKL---PNLLYHKEILPYNHLDFIWAMDAPQEV 389

BLAST of Moc04g07190 vs. ExPASy Swiss-Prot
Match: Q5VXJ0 (Lipase member K OS=Homo sapiens OX=9606 GN=LIPK PE=2 SV=2)

HSP 1 Score: 198.0 bits (502), Expect = 2.1e-49
Identity = 111/369 (30.08%), Postives = 201/369 (54.47%), Query Frame = 0

Query: 33  HRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDG-DLKKIKGPPILLLH 92
           + A+ E     SQ++   GYP EE+ + TKDG++LG+ R+    G   +    P + L H
Sbjct: 25  NNANPEANMNISQIISYWGYPYEEYDVTTKDGYILGIYRIPHGRGCPGRTAPKPAVYLQH 84

Query: 93  GLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWED 152
           GL+ +   W  N    SL F+LA++G+DVW+GN RG  WS  H+ LS    ++W +S ++
Sbjct: 85  GLIASASNWICNLPNNSLAFLLADSGYDVWLGNSRGNTWSRKHLKLSPKSPEYWAFSLDE 144

Query: 153 LALYDLAEMIDYINSLTDRK-VYVVGHSQGTIISFVAL-TQPDIAKKVEAAALLSPISYL 212
           +A YDL   I++I   T +K +Y VGHSQGT I+F+A  T P++AKK++    L+P+  +
Sbjct: 145 MAKYDLPATINFIIEKTGQKRLYYVGHSQGTTIAFIAFSTNPELAKKIKIFFALAPVVTV 204

Query: 213 DHITAPLVRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLV---DCNNILSSIT 272
            +  +P+ +L   L    + +  G    +  + +   +   +C++ +    C+N L +++
Sbjct: 205 KYTQSPMKKL-TTLSRRVVKVLFGDKMFHPHTLFDQFIATKVCNRKLFRRICSNFLFTLS 264

Query: 273 GKNCY-LNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKP 332
           G +   LN SR D +    P  TS +N+ H  Q +  G    +D+G  + N+  + +  P
Sbjct: 265 GFDPQNLNMSRLDVYLSHNPAGTSVQNMLHWAQAVNSGQLQAFDWGNSDQNMMHFHQLTP 324

Query: 333 PAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRA 392
           P +++ ++     +W   GG D ++D  D+++ + ++ ++     + +Y HVDF L   A
Sbjct: 325 PLYNITKMEVPTAIW--NGGQDIVADPKDVENLLPQIANLIYYKLIPHYNHVDFYLGEDA 384

Query: 393 KEDVYDPMI 394
            +++Y  +I
Sbjct: 385 PQEIYQDLI 390

BLAST of Moc04g07190 vs. ExPASy TrEMBL
Match: A0A6J1D3W4 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017353 PE=3 SV=1)

HSP 1 Score: 835.5 bits (2157), Expect = 9.5e-239
Identity = 405/405 (100.00%), Postives = 405/405 (100.00%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ
Sbjct: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
           TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV
Sbjct: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240
           TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK
Sbjct: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240

Query: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300
           SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI
Sbjct: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300

Query: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360
           RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE
Sbjct: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360

Query: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 405

BLAST of Moc04g07190 vs. ExPASy TrEMBL
Match: A0A6J1D5Z8 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1)

HSP 1 Score: 782.7 bits (2020), Expect = 7.3e-223
Identity = 377/405 (93.09%), Postives = 392/405 (96.79%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDS DRS +HSIL R SSEQ+TLCSQLVEPAGYPCEEHKIQ
Sbjct: 1   MAKPLLALMFLLYFALHATDDSYDRSADHSILPRGSSEQKTLCSQLVEPAGYPCEEHKIQ 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
           TKDGFLLGLQRVSSYDGDLK+I+GPPILLLHGLLMAGDAWFLNSA+QSLGFILA+NGFDV
Sbjct: 61  TKDGFLLGLQRVSSYDGDLKEIQGPPILLLHGLLMAGDAWFLNSADQSLGFILADNGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240
           TI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPLVRLMVDLHLDTMIL MGFH+LNFK
Sbjct: 181 TIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPLVRLMVDLHLDTMILAMGFHELNFK 240

Query: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300
           SDWGT+LLDMLCDQLV+C +ILS ITGKNCYLNGSRFD FFE+EPHPTSAKNL HLFQMI
Sbjct: 241 SDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGSRFDIFFEYEPHPTSAKNLRHLFQMI 300

Query: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360
           RKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE
Sbjct: 301 RKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360

Query: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF KSLGK SSL
Sbjct: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFIKSLGKFSSL 405

BLAST of Moc04g07190 vs. ExPASy TrEMBL
Match: A0A6J1D6E7 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 1.8e-221
Identity = 377/406 (92.86%), Postives = 392/406 (96.55%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDS DRS +HSIL R SSEQ+TLCSQLVEPAGYPCEEHKIQ
Sbjct: 1   MAKPLLALMFLLYFALHATDDSYDRSADHSILPRGSSEQKTLCSQLVEPAGYPCEEHKIQ 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
           TKDGFLLGLQRVSSYDGDLK+I+GPPILLLHGLLMAGDAWFLNSA+QSLGFILA+NGFDV
Sbjct: 61  TKDGFLLGLQRVSSYDGDLKEIQGPPILLLHGLLMAGDAWFLNSADQSLGFILADNGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEH+KKFWNWSWE+LALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHEKKFWNWSWEELALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDT-MILTMGFHQLNF 240
           TI+SFVAL+QPDIAKKVEAAALL PISYLDHITAPLVRLMVDLHLDT MIL MGFH+LNF
Sbjct: 181 TIMSFVALSQPDIAKKVEAAALLGPISYLDHITAPLVRLMVDLHLDTQMILAMGFHELNF 240

Query: 241 KSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQM 300
           KSDWGT+LLDMLCDQLV+C +ILS ITGKNCYLNGSRFD FFE+EPHPTSAKNL HLFQM
Sbjct: 241 KSDWGTDLLDMLCDQLVNCIDILSLITGKNCYLNGSRFDIFFEYEPHPTSAKNLRHLFQM 300

Query: 301 IRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK 360
           IRKGT SRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK
Sbjct: 301 IRKGTLSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK 360

Query: 361 ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKF KSLGK SSL
Sbjct: 361 ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFIKSLGKFSSL 406

BLAST of Moc04g07190 vs. ExPASy TrEMBL
Match: A0A6J1D6F2 (Lipase OS=Momordica charantia OX=3673 GN=LOC111017353 PE=3 SV=1)

HSP 1 Score: 742.3 bits (1915), Expect = 1.1e-210
Identity = 368/405 (90.86%), Postives = 368/405 (90.86%), Query Frame = 0

Query: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQ 60
           MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHK  
Sbjct: 1   MAKPLLALMFLLYFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHK-- 60

Query: 61  TKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDV 120
                                              AGDAWFLNSAEQSLGFILAENGFDV
Sbjct: 61  -----------------------------------AGDAWFLNSAEQSLGFILAENGFDV 120

Query: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180
           WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG
Sbjct: 121 WIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQG 180

Query: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240
           TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK
Sbjct: 181 TIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFK 240

Query: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300
           SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI
Sbjct: 241 SDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMI 300

Query: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360
           RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE
Sbjct: 301 RKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKE 360

Query: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL
Sbjct: 361 LDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 368

BLAST of Moc04g07190 vs. ExPASy TrEMBL
Match: A0A6J1GLC0 (Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 6.7e-200
Identity = 339/406 (83.50%), Postives = 374/406 (92.12%), Query Frame = 0

Query: 1   MAKPLLALMFLL-YFALHATDDSSDRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKI 60
           MAKPL+A++FLL +FALH+ DDSS RS E+SIL R  S+Q++LCSQLV+PAGYPC EH+I
Sbjct: 1   MAKPLMAMVFLLCFFALHSADDSSYRSLEYSILPRGLSDQKSLCSQLVQPAGYPCAEHQI 60

Query: 61  QTKDGFLLGLQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFD 120
           QTKDGFLLGLQRVSS DGDL+K KGPPILLLHGL M GDAWFLNSA +SLGFILA+NGFD
Sbjct: 61  QTKDGFLLGLQRVSSCDGDLEKQKGPPILLLHGLFMGGDAWFLNSARESLGFILADNGFD 120

Query: 121 VWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQ 180
           VWIGNVRGTRWSHGH SLSE +K+FW+WSWE+LALYDLA MIDYINSLT RK+YVVGHSQ
Sbjct: 121 VWIGNVRGTRWSHGHTSLSEDEKEFWDWSWEELALYDLAGMIDYINSLTKRKLYVVGHSQ 180

Query: 181 GTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQLNF 240
           GTI+SF ALTQPDIA+KVEAAALLSPISYL+HITAPLVRLMVD HLDT+IL  GFH+LNF
Sbjct: 181 GTIMSFAALTQPDIAEKVEAAALLSPISYLEHITAPLVRLMVDTHLDTIILAAGFHELNF 240

Query: 241 KSDWGTNLLDMLCDQLVDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQM 300
           KSDWGT LLD LCD+LV+C N+LSSITG+NC LNGSRFD FFE+EPHP+SAKNLHHLFQM
Sbjct: 241 KSDWGTVLLDDLCDRLVNCINVLSSITGENCCLNGSRFDLFFEYEPHPSSAKNLHHLFQM 300

Query: 301 IRKGTFSRYDYGLLNNLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIK 360
           IRKGTFSRYDYGLL NLRVYG+ KPPAFDL RIPKSLPLWMAYGG+DELSDWTDLQHTIK
Sbjct: 301 IRKGTFSRYDYGLLKNLRVYGQRKPPAFDLSRIPKSLPLWMAYGGSDELSDWTDLQHTIK 360

Query: 361 ELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFKSLGKSSSL 406
           EL SVPELVYLENYGHVDFILS++AKEDVYDPMIKF KSLGKS +L
Sbjct: 361 ELKSVPELVYLENYGHVDFILSMKAKEDVYDPMIKFLKSLGKSGNL 406

BLAST of Moc04g07190 vs. TAIR 10
Match: AT2G15230.1 (lipase 1 )

HSP 1 Score: 502.3 bits (1292), Expect = 3.7e-142
Identity = 229/378 (60.58%), Postives = 297/378 (78.57%), Query Frame = 0

Query: 27  TEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKIKGPP 86
           T+  +LH   S   +LC+ L+ PA Y C EH IQTKDG++L LQRV+S    L+   GPP
Sbjct: 19  TQSHLLH--GSPVNSLCADLIHPANYSCTEHSIQTKDGYILALQRVASLGPRLQ--SGPP 78

Query: 87  ILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWN 146
           +LL HGL MAGD WFLNS ++SLGFILA++GFDVW+GNVRGTR+S+GH++LS+ DK+FW+
Sbjct: 79  VLLQHGLFMAGDVWFLNSPKESLGFILADHGFDVWVGNVRGTRYSYGHVTLSDTDKEFWD 138

Query: 147 WSWEDLALYDLAEMIDYINSLTDRKVYVVGHSQGTIISFVALTQPDIAKKVEAAALLSPI 206
           WSW+DLA+YDLAEMI Y+ S+++ K+++VGHSQGTI+SF ALTQP +A+ VEAAALL PI
Sbjct: 139 WSWQDLAMYDLAEMIQYLYSISNSKIFLVGHSQGTIMSFAALTQPHVAEMVEAAALLCPI 198

Query: 207 SYLDHITAPLVRLMVDLHLDTMILTMGFHQLNFKSDWGTNLLDMLCDQLVDCNNILSSIT 266
           SYLDH+TAPLV  MV +HLD M++ +G HQ+NF+SD    L+D LC+  +DC + L+SIT
Sbjct: 199 SYLDHVTAPLVERMVFMHLDQMVVALGLHQINFRSDMLVKLVDSLCEGHMDCTDFLTSIT 258

Query: 267 GKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLNNLRVYGRSKPPA 326
           G NC  N S+ +++ ++EPHP+S KN+ HLFQMIRKGTF++YDYG   NLR YG SKPP 
Sbjct: 259 GTNCCFNASKIEYYLDYEPHPSSVKNIRHLFQMIRKGTFAQYDYGYFKNLRTYGLSKPPE 318

Query: 327 FDLRRIPKSLPLWMAYGGNDELSDWTDLQHTIKELDSVPELVYLENYGHVDFILSVRAKE 386
           F L  IP SLP+WM YGG D L+D TD++HT+ EL S PEL+YLE+YGH+DF+L   AKE
Sbjct: 319 FILSHIPASLPMWMGYGGTDGLADVTDVEHTLAELPSSPELLYLEDYGHIDFVLGSSAKE 378

Query: 387 DVYDPMIKFFKSLGKSSS 405
           DVY  MI+FF++  KSSS
Sbjct: 379 DVYKHMIQFFRAKVKSSS 392

BLAST of Moc04g07190 vs. TAIR 10
Match: AT5G14180.1 (Myzus persicae-induced lipase 1 )

HSP 1 Score: 289.7 bits (740), Expect = 3.7e-78
Identity = 155/408 (37.99%), Postives = 242/408 (59.31%), Query Frame = 0

Query: 6   LALMFLLYFALHATDDSSDRSTEHSILHRASSEQRT----LCSQLVEPAGYPCEEHKIQT 65
           LAL  L++FAL +      R T   +       QRT    +C+  V   GY CEEH + T
Sbjct: 14  LALSVLIFFAL-SLKTLEARGTFGRL--AGQPPQRTAAGGICASSVHIFGYKCEEHDVVT 73

Query: 66  KDGFLLGLQRVSSYDGDLKKIKG-----PPILLLHGLLMAGDAWFLNSAEQSLGFILAEN 125
           +DG++L +QR+   +G    + G      P+L+ HG+L+ G +W LN A+Q+L  ILA+ 
Sbjct: 74  QDGYILNMQRIP--EGRAGAVAGDGGKRQPVLIQHGILVDGMSWLLNPADQNLPLILADQ 133

Query: 126 GFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSLTDRKVYVVG 185
           GFDVW+GN RGTR+S  H  L+   + FWNW+W++L  YDL  M D+I+ LT +K++ +G
Sbjct: 134 GFDVWMGNTRGTRFSRRHKYLNPSQRAFWNWTWDELVSYDLPAMFDHIHGLTGQKIHYLG 193

Query: 186 HSQGTIISFVALTQPDIAKKVEAAALLSPISYLDHITAPLVRLMVDLHLDTMILTMGFHQ 245
           HS GT+I F + ++  +  +V +AA+LSP++YL H+T  +  +     L      +G+ +
Sbjct: 194 HSLGTLIGFASFSEKGLVDQVRSAAMLSPVAYLSHMTTVIGDIAAKTFLAEATSILGWPE 253

Query: 246 LNFKSDWGTNLLDMLCDQL-VDCNNILSSITGKNCYLNGSRFDFFFEFEPHPTSAKNLHH 305
            N KS    + +  +C +  +DC +++S ITGKNC LN S  D F   EP  TS KN+ H
Sbjct: 254 FNPKSGLVGDFIKAICLKAGIDCYDLVSVITGKNCCLNASTIDLFLANEPQSTSTKNMIH 313

Query: 306 LFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFDLRRIPKSLPLWMAYGGNDELSDWTDL 365
           L Q +R     +Y+YG  + N++ YG++ PPA+++  IP  LPL+ +YGG D L+D  D+
Sbjct: 314 LAQTVRDKELRKYNYGSSDRNIKHYGQAIPPAYNISAIPHELPLFFSYGGLDSLADVKDV 373

Query: 366 -----QHTIKELDSVPELVYLENYGHVDFILSVRAKEDVYDPMIKFFK 398
                Q    ++D +  + ++++Y H DFI+ V AK+ VY+ +  FFK
Sbjct: 374 EFLLDQFKYHDIDKM-NVQFVKDYAHADFIMGVTAKDVVYNQVATFFK 415

BLAST of Moc04g07190 vs. TAIR 10
Match: AT1G73920.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 6.9e-16
Identity = 93/401 (23.19%), Postives = 163/401 (40.65%), Query Frame = 0

Query: 32  LHRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKIKGPPILLLH 91
           L+ + +     C  ++   GYP E  ++ T DG++L L+R+   D          + L H
Sbjct: 285 LYNSMNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDA------RKAVFLQH 344

Query: 92  GLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWED 151
           G+L +   W  N    S  F   + G+DV++GN RG   S  H++ +   K+FW +S  +
Sbjct: 345 GVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINE 404

Query: 152 LALYDLAEMIDYINSLTDR-------------------KVYVVGHSQGTIISFVALTQPD 211
               D+  MI+ I+ +                      K+  + HS G     + +    
Sbjct: 405 HGTEDIPAMIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRK 464

Query: 212 IAKK---VEAAALLSPISYLD--------------HITAPLVRLMVDLHLDTMILTMGFH 271
           I +K   +    LLSP  + +               I+  L R++   ++ T    M  +
Sbjct: 465 IKEKPHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLN 524

Query: 272 QL-----NFKSDWGTNLLDMLCDQLV--DCNNILSSITGKNCYLNGSRFDFFFEFEPHPT 331
           +L     N+ +  G  L+  L   +V  D +N +  +   +  +N          +    
Sbjct: 525 KLARDFHNYPALGG--LVQTLMSYVVGGDSSNWVGVLGLPHYNMN----------DMPAV 584

Query: 332 SAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFDLRRIPK--SLPLWMAYGGN 384
           S +   HL Q+   G F  YDYG  + N+ VYG  +P   DL    K   +P+ +  G N
Sbjct: 585 SFRVAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRN 644

BLAST of Moc04g07190 vs. TAIR 10
Match: AT1G73920.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 6.9e-16
Identity = 93/401 (23.19%), Postives = 163/401 (40.65%), Query Frame = 0

Query: 32  LHRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLGLQRVSSYDGDLKKIKGPPILLLH 91
           L+ + +     C  ++   GYP E  ++ T DG++L L+R+   D          + L H
Sbjct: 216 LYNSMNTDTRTCQDVITELGYPYEAIRVITSDGYVLVLERIPRRDA------RKAVFLQH 275

Query: 92  GLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGTRWSHGHISLSEHDKKFWNWSWED 151
           G+L +   W  N    S  F   + G+DV++GN RG   S  H++ +   K+FW +S  +
Sbjct: 276 GVLDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL-VSRDHVNKNISSKEFWRYSINE 335

Query: 152 LALYDLAEMIDYINSLTDR-------------------KVYVVGHSQGTIISFVALTQPD 211
               D+  MI+ I+ +                      K+  + HS G     + +    
Sbjct: 336 HGTEDIPAMIEKIHEIKTTELKLCQPNIDEEINQEEPYKLCAICHSLGGAAILMYVITRK 395

Query: 212 IAKK---VEAAALLSPISYLD--------------HITAPLVRLMVDLHLDTMILTMGFH 271
           I +K   +    LLSP  + +               I+  L R++   ++ T    M  +
Sbjct: 396 IKEKPHRLSRLILLSPAGFHEDSNLGFTIVEYIFLFISPVLARIVPAFYIPTRFFRMLLN 455

Query: 272 QL-----NFKSDWGTNLLDMLCDQLV--DCNNILSSITGKNCYLNGSRFDFFFEFEPHPT 331
           +L     N+ +  G  L+  L   +V  D +N +  +   +  +N          +    
Sbjct: 456 KLARDFHNYPALGG--LVQTLMSYVVGGDSSNWVGVLGLPHYNMN----------DMPAV 515

Query: 332 SAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKPPAFDLRRIPK--SLPLWMAYGGN 384
           S +   HL Q+   G F  YDYG  + N+ VYG  +P   DL    K   +P+ +  G N
Sbjct: 516 SFRVAQHLAQIKHTGKFRMYDYGSRSANMEVYGSPEP--LDLGESYKFIDVPVDLVAGRN 575

BLAST of Moc04g07190 vs. TAIR 10
Match: AT1G18460.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 75.9 bits (185), Expect = 8.5e-14
Identity = 103/423 (24.35%), Postives = 178/423 (42.08%), Query Frame = 0

Query: 11  LLYFALHATDDSS--DRSTEHSILHRASSEQRTLCSQLVEPAGYPCEEHKIQTKDGFLLG 70
           ++  A+   +DSS  +R T  S+    +++ RT C  ++   GYP E  ++ T DG+ L 
Sbjct: 264 IIQTAILGDNDSSLTERRTTTSLY---NTDTRT-CQDVITELGYPYEAIRVVTSDGYGLL 323

Query: 71  LQRVSSYDGDLKKIKGPPILLLHGLLMAGDAWFLNSAEQSLGFILAENGFDVWIGNVRGT 130
           L+R+   D          + L HG++ +   W  N    S  F   + G+DV++GN RG 
Sbjct: 324 LERIPRRDA------RKAVYLQHGVMDSSMGWVSNGVVGSPAFAAYDQGYDVFLGNFRGL 383

Query: 131 RWSHGHISLSEHDKKFWNWSWEDLALYDLAEMIDYINSL--TDRKVY------------- 190
             S  H+  +   K FW +S  + A  D+  MI+ I+ +  ++ K+Y             
Sbjct: 384 -VSRDHVKKNISSKDFWRYSINEHATEDIPAMIEKIHEIKTSELKLYQPTMEEVVNEDQP 443

Query: 191 ----VVGHSQGTIISFVALTQPDIAKK---VEAAALLSP-------------ISYLDHIT 250
               VV HS G     + +    I +K   +    LLSP             + Y     
Sbjct: 444 YKLCVVSHSLGGAAVLMYVITRKIEEKPHRLSRLILLSPAGFHYDSNMCFTLMEYTFLFL 503

Query: 251 AP-LVRLMVDLHLDTMILTMGFHQL-----NFKSDWGTNLLDMLCDQLV--DCNNILSSI 310
            P L R++   ++ T    M  ++L     N+ +  G  L+  L   +V  D +N +  +
Sbjct: 504 GPVLSRIVPAFYIPTKFFRMLLNKLARDFHNYPAVGG--LVQTLMSYVVGGDSSNWVGVM 563

Query: 311 TGKNCYLNGSRFDFFFEFEPHPTSAKNLHHLFQMIRKGTFSRYDYGLLN-NLRVYGRSKP 370
              +  +N          +    S +   HL Q+   G F  +DYG  + N+ VYG  +P
Sbjct: 564 GLPHYNMN----------DMPGISFRVAQHLAQIKHSGKFKMFDYGSSSANMDVYGSPEP 623

Query: 371 PAFDLRRIPK--SLPLWMAYGGNDELSDWTDLQHTIKEL-DSVPELVYLE-NYGHVDFIL 384
              DL        +P+ +  G  D++   + ++   + + DS  ++ Y E  Y H+DF  
Sbjct: 624 --LDLGEFYGLIDVPVDLVAGKKDKVIRPSMVRKHYRVMRDSGVDVSYNEFEYAHLDFTF 661

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022148780.12.0e-238100.00triacylglycerol lipase 1-like isoform X1 [Momordica charantia][more]
XP_022148777.11.5e-22293.09triacylglycerol lipase 1-like isoform X2 [Momordica charantia][more]
XP_022148776.13.7e-22192.86triacylglycerol lipase 1-like isoform X1 [Momordica charantia][more]
XP_022148781.12.3e-21090.86triacylglycerol lipase 1-like isoform X2 [Momordica charantia][more]
XP_022952782.11.4e-19983.50triacylglycerol lipase 1 isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q71DJ55.2e-14160.58Triacylglycerol lipase 1 OS=Arabidopsis thaliana OX=3702 GN=LIP1 PE=1 SV=1[more]
Q67ZU15.3e-7737.99Triacylglycerol lipase 2 OS=Arabidopsis thaliana OX=3702 GN=LIP2 PE=2 SV=1[more]
P046341.1e-5032.60Gastric triacylglycerol lipase OS=Rattus norvegicus OX=10116 GN=Lipf PE=1 SV=1[more]
Q9CPP72.1e-4933.70Gastric triacylglycerol lipase OS=Mus musculus OX=10090 GN=Lipf PE=2 SV=1[more]
Q5VXJ02.1e-4930.08Lipase member K OS=Homo sapiens OX=9606 GN=LIPK PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D3W49.5e-239100.00Lipase OS=Momordica charantia OX=3673 GN=LOC111017353 PE=3 SV=1[more]
A0A6J1D5Z87.3e-22393.09Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1[more]
A0A6J1D6E71.8e-22192.86Lipase OS=Momordica charantia OX=3673 GN=LOC111017352 PE=3 SV=1[more]
A0A6J1D6F21.1e-21090.86Lipase OS=Momordica charantia OX=3673 GN=LOC111017353 PE=3 SV=1[more]
A0A6J1GLC06.7e-20083.50Lipase OS=Cucurbita moschata OX=3662 GN=LOC111455376 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G15230.13.7e-14260.58lipase 1 [more]
AT5G14180.13.7e-7837.99Myzus persicae-induced lipase 1 [more]
AT1G73920.16.9e-1623.19alpha/beta-Hydrolases superfamily protein [more]
AT1G73920.26.9e-1623.19alpha/beta-Hydrolases superfamily protein [more]
AT1G18460.18.5e-1424.35alpha/beta-Hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006693Partial AB-hydrolase lipase domainPFAMPF04083Abhydro_lipasecoord: 44..103
e-value: 4.9E-18
score: 64.5
IPR022742Serine aminopeptidase, S33PFAMPF12146Hydrolase_4coord: 108..220
e-value: 2.1E-6
score: 27.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 30..398
e-value: 2.0E-114
score: 384.3
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 43..397
IPR025483Lipase, eukaryoticPIRSFPIRSF000862Steryl_ester_lipcoord: 3..401
e-value: 4.4E-125
score: 415.6
NoneNo IPR availablePANTHERPTHR11005LYSOSOMAL ACID LIPASE-RELATEDcoord: 29..399
NoneNo IPR availablePANTHERPTHR11005:SF118TRIACYLGLYCEROL LIPASE 1coord: 29..399

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g07190.1Moc04g07190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016042 lipid catabolic process
biological_process GO:2000033 regulation of seed dormancy process
biological_process GO:0010029 regulation of seed germination
biological_process GO:0009739 response to gibberellin
biological_process GO:0006629 lipid metabolic process
molecular_function GO:0004806 triglyceride lipase activity
molecular_function GO:0016788 hydrolase activity, acting on ester bonds