Moc04g06750 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g06750
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionDiacylglycerol O-acyltransferase
Locationchr4: 4735727 .. 4739898 (+)
RNA-Seq ExpressionMoc04g06750
SyntenyMoc04g06750
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCGTTCAAGTTCGAGAGCGACGAGCCACTGACGCCGGCCGGCCGCCTCTTCATCCGCCCGGAAATCAACCAGATTATTCACTGCGTCGTCGGAGTGCGGAACTCGCTCGATGTCGATTCGGTCAAATCTCAAATCGCCGACTCCGTCATGATTCGGCACCCTAGGTTTTCGAGCCTCTTGATTCGCGACCCGAGCGGCGCCGAGTACTGGCGCCGGACCTCCATCGATGTCGACCGCCACGTCATCGTGGTCGCCGATCATGTGACGGACGGCGCTGGCGACGACGAGAAGGCGGTGAATCAGTATTTGGCGGATTTGGCGATTTCTCCGTCGATGGACGCCGATAAGCCTCTTTGGGAGATTCACTTGCTATTGGCGCACAACTGCGTCATCTTCCGAATCCACCACGCGCTCGGCGACGGGATCTCGCTGATGTCGATGTTCTTGACTTGCTGCCGGAAGGCAGACGATCCGGACGCGCTGCCGACCATCGTTTCGGATTCGAAGGCGGCGAGGAAGACCGGGGGGCGGAGGTGGGCGGAGGAAGTACCGAAGATATTGTTGGAGTTCTTGATGACGGTGTGGTTCAGCTTCTTGTTTGTGACGGAGTTTATTCTGAGGGCTCTGTGGGTTTCTGACCGGAAAACGCCGATCTCCGGCGGCGCTGGGGTGGAGCTCTGGCCGAGGAAAGTGGCCACGGCCAAGTTCGCGGTGGACGATATGAAAGCGGTAAAGAGTGCTGTTCCCAACGCGGTAAGTTTTAAAAAAAACAAAAATATTGAACCGTGAATTTGGCTTTTTGTGAAAATTGGTTTTCTGGTTATTATTATTATTATTATTTTAAAACTATTTACGCGATAGTGAATTAAATTTAAGCTTATATCAACGACAAAATTTAAACGCTCTTTGAGTTTTTTTTTCCCTTTTTTCTTGAGAAAAAATAAAAATCCGATTCTGGGCTAGGTGTGTAGTGTGTACGTTGTTGTTGTTAGGTGAGACAATTTTTATGGGTTTTAATTCTTGTGGCCGTAATAAATGTTCTATTCTGGTTGGACTCACAGGAAAAAAAAAGTTTATTTTTGGTCGAATCTCGTCACTCAGATTTTGGTTATACTTCAAAACCTTTATTATTATTATTATTATTTTTTTATGTGGCCGTGTTAAATGTTTTATGTTGGTTGAACTCACAGGAAAAAAATAGTTTTATTTTTGGTCGAATCTGGTCATTTCGGTTGTACTTCAAAACCTTTATTTATTTATTTTGTATATGTTTAATCCACACTCAACTAAAATGAATTAAAATGTACTAAAGTGTTGAAAGTAAAAGGACTAAAATGGAAAAAAAGAAAAATATAAGAAAATAGTATTTTAACCTTTGTGGAGTTTATGGCTAGATTTGAGTGGTTTTTGTCACTGATAATTTGGATCTATTGATTTTTTTAAAAGGAAATTTTCTTGTCTTGATTTTAAAAGGTGAATTAATTTTGCCTGTATAAAGTTTCAATACTGGGTTAAAATCAATTCAGTCAATGGCCACATAGATTTCCTATTTCCTTTCTAAGAAACATTTTGTTTTTATTGTGTTTTCATCTAATCTATTTGTATTGCTTATAAAAAAAAAAAAACAGAAAAAGAAGTTTCGCCAATATGCATATATTAAGCAACAAGTTATATTTATCCAAAAAAAAAAAAAAAAAAAGAGAAAAGAGGCTTCAAAGTGGTCAAATTATTTGGTTTCTTGTCATGAGTTTCGTTTTAATTTGTTTACTTTAAATAAATTTATTTATGTCATTTTCCTTTCCTTTAAATAATAATGGAGCCCGAATTGGCAAGTAGTATGATATATGTTTTTCCTCCTTTAGATTGACTTTTCAAAGAATCCCAATTAGAAAAGGAGGCAACTATTTTTTGTTTAATGTGTAATTTCTGTTCTTGTTTTGGTAATATTATGTTTTGGTAACATAAAATATTTTTTGTCAAAAGAAAAGGCAGATAACATATTTTAAGGCATCAAAACAACATAAAAAGTGTTCATAAAAGAAGCCTTTTTAACCAGAACCAAATTTTCTATAGATGTCAACATTTTGGTTAGAGACAAAATAAGTAAAAATTAAAATGAGTTAAGAAGAATTTATGTTTTCTGCTGTTTTCTAATTTATAAAAAATCATCCTTCCAATCAAGTTCTATTATATAAAAAAAACTATCTTTTGAGCCAACGTTAGTTTAGTGTAATTATGTTTAGTTTAGTGTAATTATGTTTTCTGCTTTTAGACACGTTTTCAAGAGGTGATAACAAATCCAAAAAAATTATAAACTTTGGTTCTGGGTGGGTTGGAAAATTAAATTTAAATTTAAAAAGTGTTTTTGTGTAAGCAAAAACTAATGAAGAGAAAAAATGTGTGGGTGCAGACAATAAATGACGTTCTGTTTAGTGTGATCGGGGCAGGCTTTTCCAGGTACTTGGAGCACAGGCAGACAAAGGGTACGTCCTCTGTCTCTCCCAAATTCCACTTTTTGGTCACATATTCCTAAATCTCTTTTTAATTTCTTCTTTAATATATTCCAATTTTCCAAAACTTTTCAATTAGATGTGGCATTAAAGTATGGTTTTATTAATATATTTGAATTAATAAACATGTTATTAAGAAGAAATTAAAATTTTTTTTGGATAGGTGGGTTGGGTTGAGCTATATATGTTCAAAAAAAAAAAAAGTGTTCAGGTTGACACCAGACTACTAATGCTTTAATGATTTCTTTTAGTTAGGACTCGATGGGAGGAGTTGAGTTATGTTTAGATTAAATTTATATGTTCACGGTTGAATAATTAAGAGTACTGGTATGTTTTTTTTTTTTTTACGGTAAACAAAATGGTTATCGAATGAGATTTTCAAACACATTTTTAGCATCTCCAACCTTGTATTTGTAGGTTTGAAAGAAAGCCTTCAATTAACGGGAGTAGCCATGGTTAATCTTAGAGCTCAACCAGGATTGCAGGTTTTTACACTCTTTTTAACTTTTTCTTTTTTTCTTTTTCTCTTCTATTTTCTTTCATGGTTAACTCTTTATGATGTGTATCGTGCAGGAACTAACTGATATGATGAAAGGAAATGGAGGATCTCGATGGGGTAACAAGCTAGGGATCCTTCTTCTTCCTGTTAACTATAGCAAGAAGGCATCTAACCCCCTTCAATACATGATAAGAACTAAAAAGATGATCGATCGAAAAAAACGCACTCTCGAAGCACATTTTTCATACGGAATGGGCAAGTTTGTAATGTCGTGCTTCGGACCCAAGGTAAGAACATAGAAGTTGAAAAATTAAAGACCTATTTGGTAACGTTCTCGTTTCTTGTTTTTGCTTCATCGTTTCTATTAAAGAAAAATAAAATTGCATAATAAAATAAAATCTTTATTTACATCGGAACAAATTAGAAACGAGAACCAGAAACAAGAAACTTATTTTTATTGTTTCCAAAATTTTTATACAATTTTGAGATTGTTTCTCACTAAAAAAACATAAATAGTTGTCAAAATGTTTAGATTTCTTAATAAAACTGGAAATGAAAAACGAAAAATGAGAACATTACCAAACATGTCTTAGACACCCAATATTAAGCAACATTTTTAATGTTTTATGCTTTTATTTTTCATTTGTTTGAAACGTTTTCAAAATTGTGTACAAATTATGCAGGTAGCTTGCATGCTTAATTACAGAATTGTTTGCAATACCACATTCACAATCTCTAATGTGATTGGCCCAAGAGAGGAGATTACCATTGGAGGCAATCCCATTACATACATTAGAGCTACTTCAACAAGCTTGTCCCATGTACTACTCCCTTCCCTATTCTTTACTTCATTCCTAGTTTTAAAAGTATGATAAGAAACGGTTAAATTCATCTAACTCAAACGTTTAAATTGATGGATTGAGTAAATTTAATTCATATTTGAAGTATCTCATTAGAAATTAATATAAATATTCGATACTTTCTTACTCGTCAATTTTATGTAGGCAATAACGATGCATATGATGAGCTATGCAGGAAGAGCCGAAATGCAAATTCTTGTGGCAAAAGATATCATCCCTGACCCTGAGTTTCTTGCTCAATGCTTTGAAAATGCATTGCTTCAAATGAAGATGGCAACAACATTGGATACCAAATAA

mRNA sequence

ATGAGTTCGTTCAAGTTCGAGAGCGACGAGCCACTGACGCCGGCCGGCCGCCTCTTCATCCGCCCGGAAATCAACCAGATTATTCACTGCGTCGTCGGAGTGCGGAACTCGCTCGATGTCGATTCGGTCAAATCTCAAATCGCCGACTCCGTCATGATTCGGCACCCTAGGTTTTCGAGCCTCTTGATTCGCGACCCGAGCGGCGCCGAGTACTGGCGCCGGACCTCCATCGATGTCGACCGCCACGTCATCGTGGTCGCCGATCATGTGACGGACGGCGCTGGCGACGACGAGAAGGCGGTGAATCAGTATTTGGCGGATTTGGCGATTTCTCCGTCGATGGACGCCGATAAGCCTCTTTGGGAGATTCACTTGCTATTGGCGCACAACTGCGTCATCTTCCGAATCCACCACGCGCTCGGCGACGGGATCTCGCTGATGTCGATGTTCTTGACTTGCTGCCGGAAGGCAGACGATCCGGACGCGCTGCCGACCATCGTTTCGGATTCGAAGGCGGCGAGGAAGACCGGGGGGCGGAGGTGGGCGGAGGAAGTACCGAAGATATTGTTGGAGTTCTTGATGACGGTGTGGTTCAGCTTCTTGTTTGTGACGGAGTTTATTCTGAGGGCTCTGTGGGTTTCTGACCGGAAAACGCCGATCTCCGGCGGCGCTGGGGTGGAGCTCTGGCCGAGGAAAGTGGCCACGGCCAAGTTCGCGGTGGACGATATGAAAGCGGTAAAGAGTGCTGTTCCCAACGCGACAATAAATGACGTTCTGTTTAGTGTGATCGGGGCAGGCTTTTCCAGGTACTTGGAGCACAGGCAGACAAAGGGTTTGAAAGAAAGCCTTCAATTAACGGGAGTAGCCATGGTTAATCTTAGAGCTCAACCAGGATTGCAGGAACTAACTGATATGATGAAAGGAAATGGAGGATCTCGATGGGGTAACAAGCTAGGGATCCTTCTTCTTCCTGTTAACTATAGCAAGAAGGCATCTAACCCCCTTCAATACATGATAAGAACTAAAAAGATGATCGATCGAAAAAAACGCACTCTCGAAGCACATTTTTCATACGGAATGGGCAAGTTTGTAATGTCGTGCTTCGGACCCAAGGTAGCTTGCATGCTTAATTACAGAATTGTTTGCAATACCACATTCACAATCTCTAATGTGATTGGCCCAAGAGAGGAGATTACCATTGGAGGCAATCCCATTACATACATTAGAGCTACTTCAACAAGCTTGTCCCATGCAATAACGATGCATATGATGAGCTATGCAGGAAGAGCCGAAATGCAAATTCTTGTGGCAAAAGATATCATCCCTGACCCTGAGTTTCTTGCTCAATGCTTTGAAAATGCATTGCTTCAAATGAAGATGGCAACAACATTGGATACCAAATAA

Coding sequence (CDS)

ATGAGTTCGTTCAAGTTCGAGAGCGACGAGCCACTGACGCCGGCCGGCCGCCTCTTCATCCGCCCGGAAATCAACCAGATTATTCACTGCGTCGTCGGAGTGCGGAACTCGCTCGATGTCGATTCGGTCAAATCTCAAATCGCCGACTCCGTCATGATTCGGCACCCTAGGTTTTCGAGCCTCTTGATTCGCGACCCGAGCGGCGCCGAGTACTGGCGCCGGACCTCCATCGATGTCGACCGCCACGTCATCGTGGTCGCCGATCATGTGACGGACGGCGCTGGCGACGACGAGAAGGCGGTGAATCAGTATTTGGCGGATTTGGCGATTTCTCCGTCGATGGACGCCGATAAGCCTCTTTGGGAGATTCACTTGCTATTGGCGCACAACTGCGTCATCTTCCGAATCCACCACGCGCTCGGCGACGGGATCTCGCTGATGTCGATGTTCTTGACTTGCTGCCGGAAGGCAGACGATCCGGACGCGCTGCCGACCATCGTTTCGGATTCGAAGGCGGCGAGGAAGACCGGGGGGCGGAGGTGGGCGGAGGAAGTACCGAAGATATTGTTGGAGTTCTTGATGACGGTGTGGTTCAGCTTCTTGTTTGTGACGGAGTTTATTCTGAGGGCTCTGTGGGTTTCTGACCGGAAAACGCCGATCTCCGGCGGCGCTGGGGTGGAGCTCTGGCCGAGGAAAGTGGCCACGGCCAAGTTCGCGGTGGACGATATGAAAGCGGTAAAGAGTGCTGTTCCCAACGCGACAATAAATGACGTTCTGTTTAGTGTGATCGGGGCAGGCTTTTCCAGGTACTTGGAGCACAGGCAGACAAAGGGTTTGAAAGAAAGCCTTCAATTAACGGGAGTAGCCATGGTTAATCTTAGAGCTCAACCAGGATTGCAGGAACTAACTGATATGATGAAAGGAAATGGAGGATCTCGATGGGGTAACAAGCTAGGGATCCTTCTTCTTCCTGTTAACTATAGCAAGAAGGCATCTAACCCCCTTCAATACATGATAAGAACTAAAAAGATGATCGATCGAAAAAAACGCACTCTCGAAGCACATTTTTCATACGGAATGGGCAAGTTTGTAATGTCGTGCTTCGGACCCAAGGTAGCTTGCATGCTTAATTACAGAATTGTTTGCAATACCACATTCACAATCTCTAATGTGATTGGCCCAAGAGAGGAGATTACCATTGGAGGCAATCCCATTACATACATTAGAGCTACTTCAACAAGCTTGTCCCATGCAATAACGATGCATATGATGAGCTATGCAGGAAGAGCCGAAATGCAAATTCTTGTGGCAAAAGATATCATCCCTGACCCTGAGTTTCTTGCTCAATGCTTTGAAAATGCATTGCTTCAAATGAAGATGGCAACAACATTGGATACCAAATAA

Protein sequence

MSSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRWAEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATTLDTK
Homology
BLAST of Moc04g06750 vs. NCBI nr
Match: XP_038888558.1 (O-acyltransferase WSD1-like [Benincasa hispida])

HSP 1 Score: 796.6 bits (2056), Expect = 1.2e-226
Identity = 390/465 (83.87%), Postives = 430/465 (92.47%), Query Frame = 0

Query: 5   KFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIR 64
           KFE+DEPLTPAGRLF+RPEINQIIHCVVG++NS+D+DSVKSQIADSVMI+HPRFSSLL+R
Sbjct: 29  KFEADEPLTPAGRLFLRPEINQIIHCVVGLQNSIDIDSVKSQIADSVMIQHPRFSSLLVR 88

Query: 65  DPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEIH 124
           D +G EYWRRTSI++DRHVIVVA+ V+D   DDEKAVNQYLADLAISPSMDADKPLWEIH
Sbjct: 89  DRNGVEYWRRTSIEIDRHVIVVAERVSDEGVDDEKAVNQYLADLAISPSMDADKPLWEIH 148

Query: 125 LLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRR-WAE 184
           LLLAHNC +FRIHHALGDGISLMSMFLTCCR+ADDP+ALPTIVS+SKA R+   +R WA+
Sbjct: 149 LLLAHNCAVFRIHHALGDGISLMSMFLTCCRRADDPNALPTIVSNSKAVRRNMRQRSWAD 208

Query: 185 EVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVDDM 244
           E+PK+LLEFL  VWFSF FV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+DM
Sbjct: 209 EIPKMLLEFLFAVWFSFWFVAEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVEDM 268

Query: 245 KAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQELT 304
           KAVK AVPNATIND+LFSVIGAG SRYLEHRQ KGLKE LQLTGVAMVNLR QPGLQ+LT
Sbjct: 269 KAVKKAVPNATINDILFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLT 328

Query: 305 DMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMGKF 364
           +MMKGN GSRWGNKLG+LLLPVN  KK  +PLQY+ +TKK++DRKKRTLEAHFSYG+GK 
Sbjct: 329 NMMKGNRGSRWGNKLGVLLLPVNCHKKMLDPLQYVKKTKKLLDRKKRTLEAHFSYGIGKL 388

Query: 365 VMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITMHM 424
           VMSC GPKVAC+LNYRIVCNT+FTISNVIGPREEITIGGNP+TYIRATSTSLSHA+TMHM
Sbjct: 389 VMSCLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRATSTSLSHALTMHM 448

Query: 425 MSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMK-MATTLDTK 468
           MSYAGRAEMQILVAKDIIPDPEFLA+CFENALL+MK  A   +TK
Sbjct: 449 MSYAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTSAEVFNTK 493

BLAST of Moc04g06750 vs. NCBI nr
Match: XP_022988766.1 (O-acyltransferase WSD1-like [Cucurbita maxima])

HSP 1 Score: 781.9 bits (2018), Expect = 3.0e-222
Identity = 385/467 (82.44%), Postives = 425/467 (91.01%), Query Frame = 0

Query: 2   SSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSL 61
           SS K ESD+PLTPAGRLF+RPEINQIIHCV+G+RNS+DVDS+KSQIADSVMI+HPRFSSL
Sbjct: 3   SSSKLESDQPLTPAGRLFLRPEINQIIHCVIGLRNSIDVDSIKSQIADSVMIQHPRFSSL 62

Query: 62  LIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLW 121
           L+RDP+G EYWRRTSI+VDRHVIVV+D V+D    +E AVN YLADL+ S +MD +KPLW
Sbjct: 63  LVRDPNGVEYWRRTSIEVDRHVIVVSDPVSDDGASNENAVNDYLADLSTSSTMDINKPLW 122

Query: 122 EIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRW 181
           EIHLLLAHNCVIFRIHHALGDGISLMS+FLTCCR+ADDPDALPTIVSDSK ARK   +RW
Sbjct: 123 EIHLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDSKTARKARQKRW 182

Query: 182 AEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVD 241
           AEE+PK+LL  LMTVWFSFLFV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+
Sbjct: 183 AEELPKMLLALLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVE 242

Query: 242 DMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQE 301
           DMKAVK+AV NATINDVLFSVIGAG SRYLEHRQ   LKE LQLTGVAM+NLR QPGLQ+
Sbjct: 243 DMKAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQD 302

Query: 302 LTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMG 361
           L DMMKGN GSRWGNKLGILLLPV Y KK S+PLQYM RTKKMIDRKKR+LE++FSYG+G
Sbjct: 303 LADMMKGNNGSRWGNKLGILLLPVYYHKKMSDPLQYMKRTKKMIDRKKRSLESYFSYGIG 362

Query: 362 KFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITM 421
           KFVMS  G KVAC+LNYRI+CNT+FTISNVIGP+EEI++GGNPITYIRATSTSL HAITM
Sbjct: 363 KFVMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITM 422

Query: 422 HMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKM-ATTLDTK 468
           HMMSYAGRA+MQILVAKDII DPEFLA+CFENAL +MKM ATTL+ K
Sbjct: 423 HMMSYAGRADMQILVAKDIIHDPEFLAKCFENALFEMKMAATTLNVK 469

BLAST of Moc04g06750 vs. NCBI nr
Match: KAG7022457.1 (O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 779.6 bits (2012), Expect = 1.5e-221
Identity = 380/462 (82.25%), Postives = 424/462 (91.77%), Query Frame = 0

Query: 2   SSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSL 61
           SS K ESD+PLTPAGRLF+RPEINQIIHCV+G++NS+DVDS+KSQIADSVMI+HPRFSSL
Sbjct: 3   SSSKLESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSL 62

Query: 62  LIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLW 121
           L+RDP+G EYWRRTSI+VDRHVIVV+D V++    +E AVN YLADL+ S +MD +KPLW
Sbjct: 63  LVRDPNGVEYWRRTSIEVDRHVIVVSDPVSNDGTGNENAVNDYLADLSTSSTMDMNKPLW 122

Query: 122 EIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRW 181
           EIHLLLAHNCVIFRIHHALGDGISLMS+FLTCCR+ADDP+ALPTIVSDSK ARK+  +RW
Sbjct: 123 EIHLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRW 182

Query: 182 AEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVD 241
           AEE+PK+LLE LMTVWFSFLFV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+
Sbjct: 183 AEELPKMLLELLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVE 242

Query: 242 DMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQE 301
           DMKAVK+AV NATINDVLFSVIGAG SRYLEHRQ   LKE LQLTGVAM+NLR QPGLQ+
Sbjct: 243 DMKAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQD 302

Query: 302 LTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMG 361
           L DMMKGN GSRWGNKLGILLLPV Y KK S+PLQY+ RTKKMIDRKKR+LE++FSYG+G
Sbjct: 303 LADMMKGNNGSRWGNKLGILLLPVYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIG 362

Query: 362 KFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITM 421
           KFVMS  G KVAC+LNYRI+CNT+FTISNVIGP+EEI++GGNPITYIRATSTSL HAITM
Sbjct: 363 KFVMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITM 422

Query: 422 HMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATT 464
           HMMSYAGRA+MQILVAKDIIPDPEFLA+CFENAL +MKMA T
Sbjct: 423 HMMSYAGRADMQILVAKDIIPDPEFLAECFENALFEMKMAAT 464

BLAST of Moc04g06750 vs. NCBI nr
Match: XP_022927903.1 (O-acyltransferase WSD1-like [Cucurbita moschata])

HSP 1 Score: 778.5 bits (2009), Expect = 3.3e-221
Identity = 378/462 (81.82%), Postives = 423/462 (91.56%), Query Frame = 0

Query: 2   SSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSL 61
           SS K ESD+PLTPAGRLF+RPEINQIIHCV+G++NS+DVDS+KSQIADSVMI+HPRFSSL
Sbjct: 3   SSSKLESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSL 62

Query: 62  LIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLW 121
           L+RDP+G EYWRRTSI+VDRHVIVV+D V+D    +E AVN YLADL+ S +MD +KPLW
Sbjct: 63  LVRDPNGVEYWRRTSIEVDRHVIVVSDPVSDDGTGNENAVNDYLADLSTSSTMDMNKPLW 122

Query: 122 EIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRW 181
           EIHLLLAHNCVIFRIHHALGDGISLMS+FLTCCR+ADDP+ALPTIVSDSK ARK+  +RW
Sbjct: 123 EIHLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRW 182

Query: 182 AEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVD 241
           AEE+PK+LLE LMT+WFSFLFV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+
Sbjct: 183 AEELPKMLLELLMTLWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVE 242

Query: 242 DMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQE 301
           DMKAVK+AV NATINDVLFSVIGAG SRYLEHRQ   LKE LQLTGVAM+NLR QPGLQ+
Sbjct: 243 DMKAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQD 302

Query: 302 LTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMG 361
           L DMMKGN GSRWGNKLGILLLP+ Y KK S+PLQY+ RTKKMIDRKKR+LE++FSYG+G
Sbjct: 303 LADMMKGNNGSRWGNKLGILLLPIYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIG 362

Query: 362 KFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITM 421
           KFVMS  G KVAC+LNYRI+CNT+FTISNVIGP+EEI++GGNPITYIRATSTSL HAITM
Sbjct: 363 KFVMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITM 422

Query: 422 HMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATT 464
           HMMSYAGRA+MQILVAKDIIPDPEFL +CFENAL +MKMA T
Sbjct: 423 HMMSYAGRADMQILVAKDIIPDPEFLVECFENALFEMKMAAT 464

BLAST of Moc04g06750 vs. NCBI nr
Match: KAG6588670.1 (O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 778.1 bits (2008), Expect = 4.3e-221
Identity = 379/462 (82.03%), Postives = 423/462 (91.56%), Query Frame = 0

Query: 2   SSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSL 61
           SS K ESD+PLTPAGRLF+RPEINQIIHCV+G++NS+DVDS+KSQIADSVMI+HPRFSSL
Sbjct: 3   SSSKLESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSL 62

Query: 62  LIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLW 121
           L+RDP+G EYWRRTSI+VDRHVIVV+D V++    +E AVN YLADL+ S +MD +KPLW
Sbjct: 63  LVRDPNGVEYWRRTSIEVDRHVIVVSDPVSNDGTGNENAVNDYLADLSTSSTMDMNKPLW 122

Query: 122 EIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRW 181
           EIHLLLAHNCVIFRIHHALGDGISLMS+FLTCCR+ADDP+ALPTIVSDSK ARK+  +RW
Sbjct: 123 EIHLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRW 182

Query: 182 AEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVD 241
           AEE+PK+LLE LMTVWFSFLFV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+
Sbjct: 183 AEELPKMLLELLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVE 242

Query: 242 DMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQE 301
           DMKAVK+AV NATINDVLFSVIGAG SRYLEHRQ   LKE LQLTGVAM+NLR QPGLQ+
Sbjct: 243 DMKAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQD 302

Query: 302 LTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMG 361
           L DMMKGN GSRWGNKLGILLLPV Y KK S+PLQY+ RTKKMIDRKKR+LE++FSYG+G
Sbjct: 303 LADMMKGNNGSRWGNKLGILLLPVYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIG 362

Query: 362 KFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITM 421
           KFVMS  G KVAC+LNYRI+CNT+FTISNVIGP+EEI++GGNPITYIRATSTSL HAITM
Sbjct: 363 KFVMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITM 422

Query: 422 HMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATT 464
           HMMSYAGRA+MQILVAKDIIPDPEFLA+CFEN L +MKMA T
Sbjct: 423 HMMSYAGRADMQILVAKDIIPDPEFLAECFENVLFEMKMAAT 464

BLAST of Moc04g06750 vs. ExPASy Swiss-Prot
Match: Q9M3B2 (Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3702 GN=WSD5 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.0e-71
Identity = 163/482 (33.82%), Postives = 264/482 (54.77%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDP 66
           E ++PL+PA RLF  PE N  I  VVG++N ++ D +   I  ++M RHPRFSS L+ + 
Sbjct: 23  EEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNC 82

Query: 67  SG---AEYWRRTSIDVDRHVI---VVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPL 126
           +     + W RT++ V+ HVI   +   H+ +   D    +  Y++DL   P +D  KPL
Sbjct: 83  NNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANAD--VFLESYVSDLTTIP-LDTSKPL 142

Query: 127 WEIHLL-----LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARK 186
           WE+HLL      A N  + RIHH+LGDG+S+MS+ L C RK  +P+ LP++   ++ +  
Sbjct: 143 WEVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSG 202

Query: 187 TGGRRWAEEVPKILLEFLMTVWFSFLFV-------TEFILRALWVSDRKTPISGGAGVEL 246
           +   + +          +M +W + L V        EFI  AL++ D +TPI G   +  
Sbjct: 203 SSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFKLSK 262

Query: 247 WPRKVATAK-FAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEH---------RQTKG 306
             R     +  ++DD+K +K+A+   T+NDV+  V  AG S+YL+          R +  
Sbjct: 263 GKRMCMVHRTVSLDDIKLIKNAM-KMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSN 322

Query: 307 LKESLQLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYM 366
           + + ++L    +VNLR   G+Q+L DMM      RWGN +G ++ P + +    +PL+++
Sbjct: 323 IPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFPFSIA-LCDDPLKHL 382

Query: 367 IRTKKMIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEI 426
            R K  IDRKK +LEA  ++ +GK +++  G + A  +  R + NTT + SN++GP EEI
Sbjct: 383 RRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSFSNLVGPVEEI 442

Query: 427 TIGGNPITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQM 461
           +  G+ +TYI  +     HA+TMH  SY  +  + + V   +I DP  L   +E +L  +
Sbjct: 443 SFYGHTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHKLCDDWEESLRSI 498

BLAST of Moc04g06750 vs. ExPASy Swiss-Prot
Match: Q9M3B1 (Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3702 GN=WSD6 PE=1 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 1.0e-71
Identity = 162/491 (32.99%), Postives = 265/491 (53.97%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDP 66
           E ++PL+PA R+F  PE N  +  V+G++  +D D +   +  + +IRHPRFSS ++   
Sbjct: 23  EEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQT-LIRHPRFSSKMVSTS 82

Query: 67  SG-----AEYWRRTSIDVDRHVIVVADHVTDGA--GDDEKAVNQYLADLAISPSMDADKP 126
            G      + W RT++ V  HVI V+D  T     G+ +  +  Y+++L   P +D  KP
Sbjct: 83  VGNKKRQTQSWVRTNVVVTDHVI-VSDIQTQNIENGNADAFLETYVSNLTTVP-LDISKP 142

Query: 127 LWEIHLL-----LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAAR 186
           LW++HLL      A N  + + HH+LGDG+SLM++ L C RK  +PD LP++ + ++++ 
Sbjct: 143 LWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSSS 202

Query: 187 KTGGRRWAEEVPKILLEFLMTVWFSFLFV-------TEFILRALWVSDRKTPISGGAGVE 246
           ++             L  +M +W + + V        EFI   +++ D +TPI G     
Sbjct: 203 RSSRLMAGSRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIKGDFRFS 262

Query: 247 LWPRKVATAK-FAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEH------------- 306
              R     +  ++DD+K +K+ +   T+NDV+  V  AG S+YL+              
Sbjct: 263 KSKRMCLVHRTVSLDDIKLIKNTM-KMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGEDQ 322

Query: 307 ---RQTKGLKESLQLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKK 366
              R+   + + ++L    +VNLR   G+Q+L DMM      RWGN +G ++ P +   +
Sbjct: 323 DSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFPFSIGLR 382

Query: 367 ASNPLQYMIRTKKMIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISN 426
             +PLQ++ R K++IDRKK +LEA  ++  GKF++  FG +VA  +  R + NTT + SN
Sbjct: 383 -DDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIINRALSNTTMSFSN 442

Query: 427 VIGPREEITIGGNPITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQC 462
           +IGP EEI+  G+PITY+  +     HA+TMH  SY  +  + + V   +I DP  L   
Sbjct: 443 LIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTVISDPHRLLDD 502

BLAST of Moc04g06750 vs. ExPASy Swiss-Prot
Match: Q5KS41 (Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=3702 GN=WSD11 PE=1 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.4e-70
Identity = 164/468 (35.04%), Postives = 252/468 (53.85%), Query Frame = 0

Query: 9   DEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDPSG 68
           +EPL+P  RLF  P+ N  I   +G +   D  ++   + +  ++ HPRFSS+L  +   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGL-EHTLVNHPRFSSILEMNNGK 73

Query: 69  AEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEIHLL-- 128
              W RT + V+ HVIV    V     + ++ +  Y++ L   P MD  KPLWE+HLL  
Sbjct: 74  KPRWVRTKVKVEEHVIV--PDVDPDIENPDQYLEDYISKLTTIP-MDLSKPLWEMHLLGV 133

Query: 129 ---LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRWAE 188
               A +  I +IHH+LGDG+SLMS+ L C RK  DP+ALPT+    K    +    +  
Sbjct: 134 KTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFN 193

Query: 189 EVPKIL--LEFLMTVWF-SFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAV 248
           ++  +   L F++ + F +F+ +  F L    + D +TP+    G EL P++      + 
Sbjct: 194 KIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHRIISF 253

Query: 249 DDMKAVKSAVPNATINDVLFSVIGAGFSRYL----EHRQTKGLKES---LQLTGVAMVNL 308
           DD+K VK+A+   T+NDVL  V  AG SRYL    +   T   KES   ++L    M+NL
Sbjct: 254 DDVKLVKNAM-KMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINL 313

Query: 309 RAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLE 368
           R   G++ L DMM      RWGN  G +LLP +   + ++PL+Y+ + K  IDRKK +LE
Sbjct: 314 RPNAGIEALADMMAKKSKCRWGNLFGYILLPFSVGLE-TDPLEYVRQAKATIDRKKHSLE 373

Query: 369 AHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATST 428
           A FS    K ++   G K + +L  +++ +TT + SNV+GP+EEIT  G+P+ YI     
Sbjct: 374 AVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYISPCVF 433

Query: 429 SLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMA 462
              HA+T+H  +YA +  + +     +IPDP  +      +L  +K A
Sbjct: 434 GHPHALTLHFQTYANKVIISVTADPTVIPDPHKMCDDLVESLKMIKAA 475

BLAST of Moc04g06750 vs. ExPASy Swiss-Prot
Match: Q9M3B3 (Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3702 GN=WSD4 PE=2 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.6e-69
Identity = 162/480 (33.75%), Postives = 259/480 (53.96%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDP 66
           E ++PL+PA RLF  PE N  I  V+G ++ LD         +S  IRHPRFSS L+ D 
Sbjct: 15  EEEQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKES-FIRHPRFSSKLVTDE 74

Query: 67  SGA-EYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEIHL 126
           +G  + W RT++ V+ H  +V         +    +  Y++DL   P +D  +PLWE+HL
Sbjct: 75  NGQNQRWVRTNVVVEDH-FIVPKIKPQNIENSNAFLEDYVSDLMKIP-LDTSRPLWELHL 134

Query: 127 L-----LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRR 186
           L      A N  + +IHH++GDG+S+MS+ L C RK  +PD LP++    +++ ++    
Sbjct: 135 LDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLT 194

Query: 187 WAEEVPKILLEFLMTVWFSFLF-------VTEFILRALWVSDRKTPISGG-AGVELWPRK 246
                   LL  +  +W + +          EFI+  L+V D +TPI G     +    +
Sbjct: 195 TGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKSKQLR 254

Query: 247 VATAKFAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYL---------EHRQTKGLKESL 306
           +     ++DD+K  K+A+ N TINDV+  V  AG SRYL         ++R+ K L + +
Sbjct: 255 LVHRTVSLDDIKLTKNAM-NMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRI 314

Query: 307 QLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKK 366
           +L    +VNLR   G+Q+L DMM+     RWGN  G ++ P + + +  +PL+++   +K
Sbjct: 315 RLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFPFSIALR-DDPLEHLEIAQK 374

Query: 367 MIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGN 426
            I RKK +  A  +Y   + ++   G KVA  +  R+V NTT T SN++GP EE++  G+
Sbjct: 375 TISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVSFYGH 434

Query: 427 PITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATT 464
           PITY  +++    HA+T+H  SY  +  + ++V   +I DP  L   +E +L  +K A T
Sbjct: 435 PITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRSIKAAVT 489

BLAST of Moc04g06750 vs. ExPASy Swiss-Prot
Match: Q9C7H4 (Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=WSD2 PE=2 SV=1)

HSP 1 Score: 248.8 bits (634), Expect = 1.2e-64
Identity = 163/473 (34.46%), Postives = 244/473 (51.59%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIR-- 66
           E++EP++P  RLF  P ++      +G +   +  ++   I ++ +I HPRFSS+L+   
Sbjct: 9   EAEEPVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNT-LINHPRFSSILVTGH 68

Query: 67  -DPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEI 126
            +  G   W  T I+V+ HVIV    +     + ++ +  Y +++A+SP MD  KPLWE 
Sbjct: 69  GEHKGKARWIPTKINVEEHVIV--PDIDPNIENPDEFLEDYTSNMALSP-MDMSKPLWEF 128

Query: 127 HLLL-----AHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKT-- 186
           HLL      A    + R HH+LGDG+SLMS+ L C RK  DP+A PT V+  K   K   
Sbjct: 129 HLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKKNKAKNVC 188

Query: 187 -GGRRWAEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVAT 246
                W   + +++    + V  S +F+          SD    I G  G  L   K   
Sbjct: 189 FSLVAWLWFIVRLMFHTCVEVIKSIVFICR-------ASDTSAHIMGKPGATLSANKFIH 248

Query: 247 AKFAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEHR-----QTKGLK--ESLQLTGV 306
              ++DD+K VK+A+ N T+NDVLF ++ AG SRYL  R      +K  K   ++ L GV
Sbjct: 249 RIISLDDVKMVKNAM-NMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHGV 308

Query: 307 AMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRK 366
              NLR    +++L  MM      RWGN +G +L+P+   K   +  +Y+ + K ++D K
Sbjct: 309 VFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLG-MKPQDDVFEYVRQAKTIMDGK 368

Query: 367 KRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYI 426
           K +LE  FSYG+ K  M  FG +    L  RI  +TT   SNV+GP EEI+  G+ I YI
Sbjct: 369 KHSLEPLFSYGLLKVTMEVFGLRGLKTLVKRIFGSTTMIFSNVVGPDEEISFFGHRIAYI 428

Query: 427 RATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMA 462
            A++  +  A+ + + SY  +  + I V  D+IPDP  L      AL  M  A
Sbjct: 429 AASTFGVPQALNICIQSYVDKLIINIGVDVDVIPDPHHLCDLIIEALRMMNSA 468

BLAST of Moc04g06750 vs. ExPASy TrEMBL
Match: A0A6J1JI63 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486010 PE=4 SV=1)

HSP 1 Score: 781.9 bits (2018), Expect = 1.4e-222
Identity = 385/467 (82.44%), Postives = 425/467 (91.01%), Query Frame = 0

Query: 2   SSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSL 61
           SS K ESD+PLTPAGRLF+RPEINQIIHCV+G+RNS+DVDS+KSQIADSVMI+HPRFSSL
Sbjct: 3   SSSKLESDQPLTPAGRLFLRPEINQIIHCVIGLRNSIDVDSIKSQIADSVMIQHPRFSSL 62

Query: 62  LIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLW 121
           L+RDP+G EYWRRTSI+VDRHVIVV+D V+D    +E AVN YLADL+ S +MD +KPLW
Sbjct: 63  LVRDPNGVEYWRRTSIEVDRHVIVVSDPVSDDGASNENAVNDYLADLSTSSTMDINKPLW 122

Query: 122 EIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRW 181
           EIHLLLAHNCVIFRIHHALGDGISLMS+FLTCCR+ADDPDALPTIVSDSK ARK   +RW
Sbjct: 123 EIHLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDSKTARKARQKRW 182

Query: 182 AEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVD 241
           AEE+PK+LL  LMTVWFSFLFV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+
Sbjct: 183 AEELPKMLLALLMTVWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVE 242

Query: 242 DMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQE 301
           DMKAVK+AV NATINDVLFSVIGAG SRYLEHRQ   LKE LQLTGVAM+NLR QPGLQ+
Sbjct: 243 DMKAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQD 302

Query: 302 LTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMG 361
           L DMMKGN GSRWGNKLGILLLPV Y KK S+PLQYM RTKKMIDRKKR+LE++FSYG+G
Sbjct: 303 LADMMKGNNGSRWGNKLGILLLPVYYHKKMSDPLQYMKRTKKMIDRKKRSLESYFSYGIG 362

Query: 362 KFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITM 421
           KFVMS  G KVAC+LNYRI+CNT+FTISNVIGP+EEI++GGNPITYIRATSTSL HAITM
Sbjct: 363 KFVMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITM 422

Query: 422 HMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKM-ATTLDTK 468
           HMMSYAGRA+MQILVAKDII DPEFLA+CFENAL +MKM ATTL+ K
Sbjct: 423 HMMSYAGRADMQILVAKDIIHDPEFLAKCFENALFEMKMAATTLNVK 469

BLAST of Moc04g06750 vs. ExPASy TrEMBL
Match: A0A6J1EMC4 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434667 PE=4 SV=1)

HSP 1 Score: 778.5 bits (2009), Expect = 1.6e-221
Identity = 378/462 (81.82%), Postives = 423/462 (91.56%), Query Frame = 0

Query: 2   SSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSL 61
           SS K ESD+PLTPAGRLF+RPEINQIIHCV+G++NS+DVDS+KSQIADSVMI+HPRFSSL
Sbjct: 3   SSSKLESDQPLTPAGRLFLRPEINQIIHCVIGLQNSIDVDSIKSQIADSVMIQHPRFSSL 62

Query: 62  LIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLW 121
           L+RDP+G EYWRRTSI+VDRHVIVV+D V+D    +E AVN YLADL+ S +MD +KPLW
Sbjct: 63  LVRDPNGVEYWRRTSIEVDRHVIVVSDPVSDDGTGNENAVNDYLADLSTSSTMDMNKPLW 122

Query: 122 EIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRW 181
           EIHLLLAHNCVIFRIHHALGDGISLMS+FLTCCR+ADDP+ALPTIVSDSK ARK+  +RW
Sbjct: 123 EIHLLLAHNCVIFRIHHALGDGISLMSLFLTCCRRADDPNALPTIVSDSKTARKSRRQRW 182

Query: 182 AEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVD 241
           AEE+PK+LLE LMT+WFSFLFV EFILRALWVSDRKTPISGG GVELWPRKVATAKFAV+
Sbjct: 183 AEELPKMLLELLMTLWFSFLFVGEFILRALWVSDRKTPISGGDGVELWPRKVATAKFAVE 242

Query: 242 DMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQE 301
           DMKAVK+AV NATINDVLFSVIGAG SRYLEHRQ   LKE LQLTGVAM+NLR QPGLQ+
Sbjct: 243 DMKAVKNAVSNATINDVLFSVIGAGLSRYLEHRQPNSLKEGLQLTGVAMINLREQPGLQD 302

Query: 302 LTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMG 361
           L DMMKGN GSRWGNKLGILLLP+ Y KK S+PLQY+ RTKKMIDRKKR+LE++FSYG+G
Sbjct: 303 LADMMKGNNGSRWGNKLGILLLPIYYHKKTSDPLQYLKRTKKMIDRKKRSLESYFSYGIG 362

Query: 362 KFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITM 421
           KFVMS  G KVAC+LNYRI+CNT+FTISNVIGP+EEI++GGNPITYIRATSTSL HAITM
Sbjct: 363 KFVMSWLGAKVACILNYRIMCNTSFTISNVIGPKEEISVGGNPITYIRATSTSLPHAITM 422

Query: 422 HMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATT 464
           HMMSYAGRA+MQILVAKDIIPDPEFL +CFENAL +MKMA T
Sbjct: 423 HMMSYAGRADMQILVAKDIIPDPEFLVECFENALFEMKMAAT 464

BLAST of Moc04g06750 vs. ExPASy TrEMBL
Match: A0A6J1GLG7 (Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455065 PE=4 SV=1)

HSP 1 Score: 762.3 bits (1967), Expect = 1.2e-216
Identity = 378/468 (80.77%), Postives = 416/468 (88.89%), Query Frame = 0

Query: 1   MSSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSS 60
           M S KFE+DEPLTPAGRLF+RPEINQIIHCV+GVR+ +DVDSVKSQIA SVM++HPRFSS
Sbjct: 1   MDSSKFETDEPLTPAGRLFLRPEINQIIHCVIGVRDLIDVDSVKSQIAGSVMVQHPRFSS 60

Query: 61  LLIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPL 120
           LL+RD +G EYWRRTSI+VDRHVIVVA+ V D    DEKAVN+YLADLAI+ SMDADKPL
Sbjct: 61  LLVRDQNGVEYWRRTSIEVDRHVIVVAEPVLDDGASDEKAVNEYLADLAINSSMDADKPL 120

Query: 121 WEIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRR 180
           WEIHLLLAH C IFRIHHALGDGISLMSMFLTCCR+ADDP+ALPTIV DS++ARKT   R
Sbjct: 121 WEIHLLLAHKCAIFRIHHALGDGISLMSMFLTCCRRADDPNALPTIVLDSRSARKTRRWR 180

Query: 181 WAEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAV 240
           WAEE+PK LL     VWF+FLFV EFILRALWVSDRK+PISGG GVELWPRK+ATAKFAV
Sbjct: 181 WAEEIPKTLL----AVWFNFLFVVEFILRALWVSDRKSPISGGDGVELWPRKMATAKFAV 240

Query: 241 DDMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQ 300
           +DMKAVK AV N T+NDVLFSVIGAG SRYLEHRQ KGLKE LQLTGVAMVNLR Q GLQ
Sbjct: 241 EDMKAVKKAVANVTMNDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQRGLQ 300

Query: 301 ELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGM 360
           ELT+MMKGN G  WGNKLGILLLPVNY KK  +PLQ ++RTKKM+DRKKR+ EAHFSYG+
Sbjct: 301 ELTEMMKGNRGFGWGNKLGILLLPVNYEKKTLDPLQCVMRTKKMLDRKKRSFEAHFSYGV 360

Query: 361 GKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAIT 420
           GKF+MSC GPKVA +LNYRIVCNT+FTISNVIGPREEITIGGNPITYIR+TSTSL H IT
Sbjct: 361 GKFIMSCLGPKVASILNYRIVCNTSFTISNVIGPREEITIGGNPITYIRSTSTSLPHTIT 420

Query: 421 MHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMK-MATTLDTK 468
           MHM+SYAGRAEMQILVAKDIIPDPEFLA+C EN LL+MK  ATT +TK
Sbjct: 421 MHMVSYAGRAEMQILVAKDIIPDPEFLAECLENVLLEMKTAATTFETK 464

BLAST of Moc04g06750 vs. ExPASy TrEMBL
Match: A0A0A0K4Q2 (Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G073550 PE=4 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.6e-216
Identity = 379/463 (81.86%), Postives = 415/463 (89.63%), Query Frame = 0

Query: 8   SDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDPS 67
           SDEPLTPAGRLF+RPEINQIIHC+VG++NS+DVDSVKSQIADS+MI+HPRFSSLL+RD +
Sbjct: 3   SDEPLTPAGRLFLRPEINQIIHCLVGLKNSIDVDSVKSQIADSIMIQHPRFSSLLVRDRN 62

Query: 68  GAEYWRRTSIDVDRHVIVVADHVTDGAG--DDEKAVNQYLADLAISPSMDADKPLWEIHL 127
           G EYWRRTSI+VDRHVIVV+D V+D  G  +DEKA N+YLADLAIS SMD  KPLWEIHL
Sbjct: 63  GVEYWRRTSIEVDRHVIVVSDPVSDDVGGVNDEKAANEYLADLAISSSMDYSKPLWEIHL 122

Query: 128 LLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRWAEEV 187
           LLAHNC +FRIHHALGDGISLMS+FLTCCR+ADDPDALPTIVSD KA R   G R     
Sbjct: 123 LLAHNCAVFRIHHALGDGISLMSLFLTCCRRADDPDALPTIVSDLKAVRT--GNRGRRSC 182

Query: 188 PKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAVDDMKA 247
            +++LEFL+TVWFS LFV EFI+RALWV DRKTPISGG GVELWPRKVATAKFA++DMKA
Sbjct: 183 GEMMLEFLLTVWFSLLFVLEFIVRALWVCDRKTPISGGDGVELWPRKVATAKFALEDMKA 242

Query: 248 VKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQELTDM 307
           VK  VPNATINDVLFSVIGAG SRYLEHRQ KGLKE LQLTGVAMVNLR QPGLQ+L+DM
Sbjct: 243 VKKGVPNATINDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQPGLQDLSDM 302

Query: 308 MKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGMGKFVM 367
           MKGN GSRWGNKLGILLLPVNY  KA +PLQY+ RTKKM+DRKKRT EAHFSYG+GK VM
Sbjct: 303 MKGNKGSRWGNKLGILLLPVNYYTKALDPLQYVKRTKKMLDRKKRTFEAHFSYGIGKLVM 362

Query: 368 SCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAITMHMMS 427
           S  GPKVAC+LNYRIVCNT+FTISNVIGPREEITIGGNP+TYIR TSTSLSHA+TMHMMS
Sbjct: 363 SFLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPVTYIRVTSTSLSHALTMHMMS 422

Query: 428 YAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMA-TTLDTK 468
           YAGRAEMQILVAKDIIPDPEFLA+CFENALL+MK A  TL TK
Sbjct: 423 YAGRAEMQILVAKDIIPDPEFLAECFENALLEMKTAGATLTTK 463

BLAST of Moc04g06750 vs. ExPASy TrEMBL
Match: A0A6J1I646 (Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471038 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.7e-216
Identity = 375/464 (80.82%), Postives = 412/464 (88.79%), Query Frame = 0

Query: 1   MSSFKFESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSS 60
           M S  FE+DEPLTPAGRLF+RPEINQIIHCV+GVR+S+DVDSVKSQIA SVM++HPRFSS
Sbjct: 1   MDSSTFETDEPLTPAGRLFLRPEINQIIHCVIGVRDSIDVDSVKSQIAGSVMVQHPRFSS 60

Query: 61  LLIRDPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPL 120
           LL+RD +G EYWRRTSI+VDRHVIVVA+ V D    DEKAVN+YLADLAI+ SMDADKPL
Sbjct: 61  LLVRDRNGVEYWRRTSIEVDRHVIVVAEPVLDDGASDEKAVNEYLADLAINSSMDADKPL 120

Query: 121 WEIHLLLAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRR 180
           WEIHLLLAH C IFRIHHALGDGISLMSMFLTCCR+ADDP+ALPTIV DS+ ARKT   R
Sbjct: 121 WEIHLLLAHKCAIFRIHHALGDGISLMSMFLTCCRRADDPNALPTIVLDSRPARKTRRWR 180

Query: 181 WAEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAV 240
           WAEE+PK+LL     VWF+FLFV EFILRALWVSDRK+PISGG GVELWPRK+ATAKFAV
Sbjct: 181 WAEEIPKMLL----AVWFNFLFVAEFILRALWVSDRKSPISGGDGVELWPRKMATAKFAV 240

Query: 241 DDMKAVKSAVPNATINDVLFSVIGAGFSRYLEHRQTKGLKESLQLTGVAMVNLRAQPGLQ 300
           +DMKAVK AV N T+NDVLFSVIGAG SRYLEHRQ KGLKE LQLTGVAMVNLR Q GLQ
Sbjct: 241 EDMKAVKKAVANVTMNDVLFSVIGAGLSRYLEHRQPKGLKEGLQLTGVAMVNLREQRGLQ 300

Query: 301 ELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLEAHFSYGM 360
           ELT MMKGN G  WGNKLGILLLPVNY KK  +PLQ ++RTKKM+DRKKR+ EAHFSYG+
Sbjct: 301 ELTQMMKGNRGFGWGNKLGILLLPVNYEKKTLDPLQCVVRTKKMLDRKKRSFEAHFSYGV 360

Query: 361 GKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATSTSLSHAIT 420
           GK +MS  GPKVAC+LNYRIVCNT+FTISNVIGPREEITIGGNPITYIR+TSTSL H IT
Sbjct: 361 GKLIMSYLGPKVACILNYRIVCNTSFTISNVIGPREEITIGGNPITYIRSTSTSLPHTIT 420

Query: 421 MHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATTL 465
           MHM+SYAGRAEMQILVAKDIIPDPEFLA+C ENALL+MK A T+
Sbjct: 421 MHMVSYAGRAEMQILVAKDIIPDPEFLAECLENALLEMKTAATI 460

BLAST of Moc04g06750 vs. TAIR 10
Match: AT3G49210.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 272.3 bits (695), Expect = 7.1e-73
Identity = 162/491 (32.99%), Postives = 265/491 (53.97%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDP 66
           E ++PL+PA R+F  PE N  +  V+G++  +D D +   +  + +IRHPRFSS ++   
Sbjct: 23  EEEQPLSPAARVFHAPEFNCYVISVIGIKKKIDPDVIIEGLKQT-LIRHPRFSSKMVSTS 82

Query: 67  SG-----AEYWRRTSIDVDRHVIVVADHVTDGA--GDDEKAVNQYLADLAISPSMDADKP 126
            G      + W RT++ V  HVI V+D  T     G+ +  +  Y+++L   P +D  KP
Sbjct: 83  VGNKKRQTQSWVRTNVVVTDHVI-VSDIQTQNIENGNADAFLETYVSNLTTVP-LDISKP 142

Query: 127 LWEIHLL-----LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAAR 186
           LW++HLL      A N  + + HH+LGDG+SLM++ L C RK  +PD LP++ + ++++ 
Sbjct: 143 LWQLHLLDLKTSDAENVAVLKFHHSLGDGMSLMALVLACMRKTSNPDELPSLPNQNRSSS 202

Query: 187 KTGGRRWAEEVPKILLEFLMTVWFSFLFV-------TEFILRALWVSDRKTPISGGAGVE 246
           ++             L  +M +W + + V        EFI   +++ D +TPI G     
Sbjct: 203 RSSRLMAGSRGDSRFLWLVMVIWSAIMLVLNTVCDALEFIATTMFLKDTETPIKGDFRFS 262

Query: 247 LWPRKVATAK-FAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEH------------- 306
              R     +  ++DD+K +K+ +   T+NDV+  V  AG S+YL+              
Sbjct: 263 KSKRMCLVHRTVSLDDIKLIKNTM-KMTVNDVVLGVSQAGLSQYLDRRYGEKKKKVGEDQ 322

Query: 307 ---RQTKGLKESLQLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKK 366
              R+   + + ++L    +VNLR   G+Q+L DMM      RWGN +G ++ P +   +
Sbjct: 323 DSKRKATDMPKRIRLRSALLVNLRPNTGIQDLADMMAKGSTCRWGNWIGYIVFPFSIGLR 382

Query: 367 ASNPLQYMIRTKKMIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISN 426
             +PLQ++ R K++IDRKK +LEA  ++  GKF++  FG +VA  +  R + NTT + SN
Sbjct: 383 -DDPLQHLRRAKRIIDRKKNSLEAALTFVAGKFILKTFGVQVAAKIINRALSNTTMSFSN 442

Query: 427 VIGPREEITIGGNPITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQC 462
           +IGP EEI+  G+PITY+  +     HA+TMH  SY  +  + + V   +I DP  L   
Sbjct: 443 LIGPIEEISFYGHPITYMAPSVYGHPHALTMHFQSYMNQMTISLTVDPTVISDPHRLLDD 502

BLAST of Moc04g06750 vs. TAIR 10
Match: AT3G49200.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 272.3 bits (695), Expect = 7.1e-73
Identity = 163/482 (33.82%), Postives = 264/482 (54.77%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDP 66
           E ++PL+PA RLF  PE N  I  VVG++N ++ D +   I  ++M RHPRFSS L+ + 
Sbjct: 23  EEEQPLSPAARLFHAPEFNCYIISVVGLKNKIEPDMIIEGIKQTLM-RHPRFSSKLVNNC 82

Query: 67  SG---AEYWRRTSIDVDRHVI---VVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPL 126
           +     + W RT++ V+ HVI   +   H+ +   D    +  Y++DL   P +D  KPL
Sbjct: 83  NNNRQEQKWVRTNVVVEDHVIIPKIQTQHIENANAD--VFLESYVSDLTTIP-LDTSKPL 142

Query: 127 WEIHLL-----LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARK 186
           WE+HLL      A N  + RIHH+LGDG+S+MS+ L C RK  +P+ LP++   ++ +  
Sbjct: 143 WEVHLLDLKTSDAENVAVLRIHHSLGDGMSMMSLVLACTRKTSNPNELPSLPYQNRPSSG 202

Query: 187 TGGRRWAEEVPKILLEFLMTVWFSFLFV-------TEFILRALWVSDRKTPISGGAGVEL 246
           +   + +          +M +W + L V        EFI  AL++ D +TPI G   +  
Sbjct: 203 SSSLKTSSRCYSRFFWLVMVLWSAALLVLNTVCDALEFIATALFLKDTETPIKGDFKLSK 262

Query: 247 WPRKVATAK-FAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEH---------RQTKG 306
             R     +  ++DD+K +K+A+   T+NDV+  V  AG S+YL+          R +  
Sbjct: 263 GKRMCMVHRTVSLDDIKLIKNAM-KMTVNDVVLGVSQAGLSQYLKRRYGEQEESKRNSSN 322

Query: 307 LKESLQLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYM 366
           + + ++L    +VNLR   G+Q+L DMM      RWGN +G ++ P + +    +PL+++
Sbjct: 323 IPKGIRLRAALLVNLRPTTGIQDLADMMTKGSKCRWGNWIGYIIFPFSIA-LCDDPLKHL 382

Query: 367 IRTKKMIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEI 426
            R K  IDRKK +LEA  ++ +GK +++  G + A  +  R + NTT + SN++GP EEI
Sbjct: 383 RRAKSTIDRKKNSLEAVLTFVVGKILLNTLGVQRAANVLNRALSNTTMSFSNLVGPVEEI 442

Query: 427 TIGGNPITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQM 461
           +  G+ +TYI  +     HA+TMH  SY  +  + + V   +I DP  L   +E +L  +
Sbjct: 443 SFYGHTVTYIAPSVYGHPHALTMHFQSYMNKLTISLTVDPTVISDPHKLCDDWEESLRSI 498

BLAST of Moc04g06750 vs. TAIR 10
Match: AT5G53390.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-71
Identity = 164/468 (35.04%), Postives = 252/468 (53.85%), Query Frame = 0

Query: 9   DEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDPSG 68
           +EPL+P  RLF  P+ N  I   +G +   D  ++   + +  ++ HPRFSS+L  +   
Sbjct: 14  EEPLSPCSRLFNSPDFNCAIIVTMGSKVKGDTPAIIHGL-EHTLVNHPRFSSILEMNNGK 73

Query: 69  AEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEIHLL-- 128
              W RT + V+ HVIV    V     + ++ +  Y++ L   P MD  KPLWE+HLL  
Sbjct: 74  KPRWVRTKVKVEEHVIV--PDVDPDIENPDQYLEDYISKLTTIP-MDLSKPLWEMHLLGV 133

Query: 129 ---LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRRWAE 188
               A +  I +IHH+LGDG+SLMS+ L C RK  DP+ALPT+    K    +    +  
Sbjct: 134 KTSNAESYAILKIHHSLGDGMSLMSLLLACTRKTSDPEALPTVAVHKKRFGPSCNSGFFN 193

Query: 189 EVPKIL--LEFLMTVWF-SFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVATAKFAV 248
           ++  +   L F++ + F +F+ +  F L    + D +TP+    G EL P++      + 
Sbjct: 194 KIWWLFVGLWFILRLLFNTFVDILMFALTIFVLRDTETPLLAKPGSELIPKRFVHRIISF 253

Query: 249 DDMKAVKSAVPNATINDVLFSVIGAGFSRYL----EHRQTKGLKES---LQLTGVAMVNL 308
           DD+K VK+A+   T+NDVL  V  AG SRYL    +   T   KES   ++L    M+NL
Sbjct: 254 DDVKLVKNAM-KMTVNDVLLGVTQAGLSRYLSRKYDQEATPKSKESMRRIRLRSAIMINL 313

Query: 309 RAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRKKRTLE 368
           R   G++ L DMM      RWGN  G +LLP +   + ++PL+Y+ + K  IDRKK +LE
Sbjct: 314 RPNAGIEALADMMAKKSKCRWGNLFGYILLPFSVGLE-TDPLEYVRQAKATIDRKKHSLE 373

Query: 369 AHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYIRATST 428
           A FS    K ++   G K + +L  +++ +TT + SNV+GP+EEIT  G+P+ YI     
Sbjct: 374 AVFSMAFFKLILKVLGLKASVVLVRKVIHSTTLSFSNVVGPKEEITFHGHPLNYISPCVF 433

Query: 429 SLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMA 462
              HA+T+H  +YA +  + +     +IPDP  +      +L  +K A
Sbjct: 434 GHPHALTLHFQTYANKVIISVTADPTVIPDPHKMCDDLVESLKMIKAA 475

BLAST of Moc04g06750 vs. TAIR 10
Match: AT3G49190.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 265.0 bits (676), Expect = 1.1e-70
Identity = 162/480 (33.75%), Postives = 259/480 (53.96%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIRDP 66
           E ++PL+PA RLF  PE N  I  V+G ++ LD         +S  IRHPRFSS L+ D 
Sbjct: 15  EEEQPLSPAARLFHAPEFNCNIISVIGFKSKLDPCVFIRGFKES-FIRHPRFSSKLVTDE 74

Query: 67  SGA-EYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEIHL 126
           +G  + W RT++ V+ H  +V         +    +  Y++DL   P +D  +PLWE+HL
Sbjct: 75  NGQNQRWVRTNVVVEDH-FIVPKIKPQNIENSNAFLEDYVSDLMKIP-LDTSRPLWELHL 134

Query: 127 L-----LAHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKTGGRR 186
           L      A N  + +IHH++GDG+S+MS+ L C RK  +PD LP++    +++ ++    
Sbjct: 135 LDLKTSDAENVAVLKIHHSVGDGMSIMSLVLACMRKTSNPDELPSLPYQYRSSSRSSLLT 194

Query: 187 WAEEVPKILLEFLMTVWFSFLF-------VTEFILRALWVSDRKTPISGG-AGVELWPRK 246
                   LL  +  +W + +          EFI+  L+V D +TPI G     +    +
Sbjct: 195 TGSRSDSRLLWLVKVIWTAVILGLNTVCDALEFIVTTLFVKDTETPIKGDFLSTKSKQLR 254

Query: 247 VATAKFAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYL---------EHRQTKGLKESL 306
           +     ++DD+K  K+A+ N TINDV+  V  AG SRYL         ++R+ K L + +
Sbjct: 255 LVHRTVSLDDIKLTKNAM-NMTINDVVLGVTQAGLSRYLARRYGEEETKNRKQKKLPKRI 314

Query: 307 QLTGVAMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKK 366
           +L    +VNLR   G+Q+L DMM+     RWGN  G ++ P + + +  +PL+++   +K
Sbjct: 315 RLRSALLVNLRPTTGIQDLADMMEKGSKCRWGNWFGYVVFPFSIALR-DDPLEHLEIAQK 374

Query: 367 MIDRKKRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGN 426
            I RKK +  A  +Y   + ++   G KVA  +  R+V NTT T SN++GP EE++  G+
Sbjct: 375 TISRKKNSYGAMLTYIFCRIIVKLLGIKVAASIINRMVSNTTMTFSNMVGPVEEVSFYGH 434

Query: 427 PITYIRATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMATT 464
           PITY  +++    HA+T+H  SY  +  + ++V   +I DP  L   +E +L  +K A T
Sbjct: 435 PITYFASSAYGHPHALTIHCQSYMNKMTITLIVDPTVISDPHRLCDDWEESLRSIKAAVT 489

BLAST of Moc04g06750 vs. TAIR 10
Match: AT1G72110.1 (O-acyltransferase (WSD1-like) family protein )

HSP 1 Score: 248.8 bits (634), Expect = 8.4e-66
Identity = 163/473 (34.46%), Postives = 244/473 (51.59%), Query Frame = 0

Query: 7   ESDEPLTPAGRLFIRPEINQIIHCVVGVRNSLDVDSVKSQIADSVMIRHPRFSSLLIR-- 66
           E++EP++P  RLF  P ++      +G +   +  ++   I ++ +I HPRFSS+L+   
Sbjct: 9   EAEEPVSPFARLFSLPGLDVFNIVTIGCKTEGNASTIVEGIKNT-LINHPRFSSILVTGH 68

Query: 67  -DPSGAEYWRRTSIDVDRHVIVVADHVTDGAGDDEKAVNQYLADLAISPSMDADKPLWEI 126
            +  G   W  T I+V+ HVIV    +     + ++ +  Y +++A+SP MD  KPLWE 
Sbjct: 69  GEHKGKARWIPTKINVEEHVIV--PDIDPNIENPDEFLEDYTSNMALSP-MDMSKPLWEF 128

Query: 127 HLLL-----AHNCVIFRIHHALGDGISLMSMFLTCCRKADDPDALPTIVSDSKAARKT-- 186
           HLL      A    + R HH+LGDG+SLMS+ L C RK  DP+A PT V+  K   K   
Sbjct: 129 HLLKLKTSHAEAVTVARFHHSLGDGMSLMSLLLACTRKTCDPEAFPTFVAPKKNKAKNVC 188

Query: 187 -GGRRWAEEVPKILLEFLMTVWFSFLFVTEFILRALWVSDRKTPISGGAGVELWPRKVAT 246
                W   + +++    + V  S +F+          SD    I G  G  L   K   
Sbjct: 189 FSLVAWLWFIVRLMFHTCVEVIKSIVFICR-------ASDTSAHIMGKPGATLSANKFIH 248

Query: 247 AKFAVDDMKAVKSAVPNATINDVLFSVIGAGFSRYLEHR-----QTKGLK--ESLQLTGV 306
              ++DD+K VK+A+ N T+NDVLF ++ AG SRYL  R      +K  K   ++ L GV
Sbjct: 249 RIISLDDVKMVKNAM-NMTVNDVLFGMVQAGLSRYLNQRYDLETSSKSRKNLHNIGLHGV 308

Query: 307 AMVNLRAQPGLQELTDMMKGNGGSRWGNKLGILLLPVNYSKKASNPLQYMIRTKKMIDRK 366
              NLR    +++L  MM      RWGN +G +L+P+   K   +  +Y+ + K ++D K
Sbjct: 309 VFFNLRPNRNIEDLAKMMAKGSKCRWGNSIGYVLIPLG-MKPQDDVFEYVRQAKTIMDGK 368

Query: 367 KRTLEAHFSYGMGKFVMSCFGPKVACMLNYRIVCNTTFTISNVIGPREEITIGGNPITYI 426
           K +LE  FSYG+ K  M  FG +    L  RI  +TT   SNV+GP EEI+  G+ I YI
Sbjct: 369 KHSLEPLFSYGLLKVTMEVFGLRGLKTLVKRIFGSTTMIFSNVVGPDEEISFFGHRIAYI 428

Query: 427 RATSTSLSHAITMHMMSYAGRAEMQILVAKDIIPDPEFLAQCFENALLQMKMA 462
            A++  +  A+ + + SY  +  + I V  D+IPDP  L      AL  M  A
Sbjct: 429 AASTFGVPQALNICIQSYVDKLIINIGVDVDVIPDPHHLCDLIIEALRMMNSA 468

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038888558.11.2e-22683.87O-acyltransferase WSD1-like [Benincasa hispida][more]
XP_022988766.13.0e-22282.44O-acyltransferase WSD1-like [Cucurbita maxima][more]
KAG7022457.11.5e-22182.25O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022927903.13.3e-22181.82O-acyltransferase WSD1-like [Cucurbita moschata][more]
KAG6588670.14.3e-22182.03O-acyltransferase WSD1, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q9M3B21.0e-7133.82Wax ester synthase/diacylglycerol acyltransferase 5 OS=Arabidopsis thaliana OX=3... [more]
Q9M3B11.0e-7132.99Wax ester synthase/diacylglycerol acyltransferase 6 OS=Arabidopsis thaliana OX=3... [more]
Q5KS411.4e-7035.04Wax ester synthase/diacylglycerol acyltransferase 11 OS=Arabidopsis thaliana OX=... [more]
Q9M3B31.6e-6933.75Wax ester synthase/diacylglycerol acyltransferase 4 OS=Arabidopsis thaliana OX=3... [more]
Q9C7H41.2e-6434.46Wax ester synthase/diacylglycerol acyltransferase 2 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A6J1JI631.4e-22282.44Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111486010 PE=... [more]
A0A6J1EMC41.6e-22181.82Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111434667 P... [more]
A0A6J1GLG71.2e-21680.77Diacylglycerol O-acyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111455065 P... [more]
A0A0A0K4Q22.6e-21681.86Diacylglycerol O-acyltransferase OS=Cucumis sativus OX=3659 GN=Csa_7G073550 PE=4... [more]
A0A6J1I6467.7e-21680.82Diacylglycerol O-acyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111471038 PE=... [more]
Match NameE-valueIdentityDescription
AT3G49210.17.1e-7332.99O-acyltransferase (WSD1-like) family protein [more]
AT3G49200.17.1e-7333.82O-acyltransferase (WSD1-like) family protein [more]
AT5G53390.11.0e-7135.04O-acyltransferase (WSD1-like) family protein [more]
AT3G49190.11.1e-7033.75O-acyltransferase (WSD1-like) family protein [more]
AT1G72110.18.4e-6634.46O-acyltransferase (WSD1-like) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR009721O-acyltransferase WSD1, C-terminalPFAMPF06974WS_DGAT_Ccoord: 314..459
e-value: 2.0E-42
score: 144.5
IPR023213Chloramphenicol acetyltransferase-like domain superfamilyGENE3D3.30.559.10coord: 11..165
e-value: 1.2E-6
score: 30.5
IPR004255O-acyltransferase, WSD1, N-terminalPFAMPF03007WES_acyltransfcoord: 67..258
e-value: 1.5E-10
score: 41.3
NoneNo IPR availablePANTHERPTHR31650:SF41O-ACYLTRANSFERASE WSD1-LIKE ISOFORM X1coord: 7..463
NoneNo IPR availableSUPERFAMILY52777CoA-dependent acyltransferasescoord: 10..150
IPR045034O-acyltransferase WSD1-likePANTHERPTHR31650O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEINcoord: 7..463

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g06750.1Moc04g06750.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019432 triglyceride biosynthetic process
biological_process GO:0045017 glycerolipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004144 diacylglycerol O-acyltransferase activity
molecular_function GO:0047196 long-chain-alcohol O-fatty-acyltransferase activity
molecular_function GO:0008374 O-acyltransferase activity