Moc04g06330 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g06330
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	salicylic acid-binding protein 2-like
Location	chr4: 4367225 .. 4369138 (+)
RNA-Seq Expression	Moc04g06330
Synteny	Moc04g06330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGAAGAACATGGGAAGAACACAGAAGCAATATTTGCATTTGCATAATCTGCTGCCATTTTTGCTTCTCCTCATTCTCCTCTCTCTCTCTTCTCCGACCCAGAGCTCGAGTCTCGGACTCCATAGTCAACCTCAGTCCTCGTCGAAAAAACTACACTTCGTGCTCGTCCATGGCGCCTGTCTCGGCGCCTGGTCGTGGTACAAGGTCACCACATACCTGCAATTGGCTGGCCACAATGTGACTGCCCTTGACATGGCTGGCGCCGGAATCAACCCGACGCAGCCCAAGTCGCTGTCTTCGCTCACCGATTACTATGAGCCATTGTTGAGGTTCATGGACGCGCTGCCCTCTGATGAGAAGGTCGTCCTGGTCGGGCACAGTCTCGGCGGGCTTGGAATCTCAATGGCCATGGAGGAGTTCCCCGAGAAGATCTCTGTTGCCATTTTTGTCACTGCAGCCATGCCTGGTCCAATCTCCAATTTAGAGGAAAAGGTTTCTAAAATCCAATCCCATTTTCTTCAATTTTCCACTCTCTCGTAAATCTAAAAGCTTAAGTTTGAGTTTGGATAAATTTAATTATATATAAATATCCCTAACACTTTTTTTCTTCTTTGATAACCAAATTAGAAGAAGATACTTGAAATGTATGGCACTTTCAACAAAAAGGATGAAGAACGATCCAAAGTTTCTGATGCAAAACTTTTTATTTTCACCGAAGAACAATTAGCAACCAAACTTTTCCCGCTCTCCCCGCCTGAGGTTCGATTCGCTAAACCGCTAGCTCACATATTATTTAGTATGTTGTAAAGAGAGTTTCGATCCATTTAGCCATTGATATGTTTGATATTTTTTTAATTTATATATTCTTGGTGAATGTTAGAGTTAATGATCGATAAACTTAGGTTGTTAACGAGTCTATAAAAGTTTATTATCAATTAATTTTAATATTCACATATAAGTATAAAATGTTAAAACATAAAAAAAACTAAAATAAAATAAAAAAGATACTTTGGACTAAAATATATAATTATTTCTTTTATATATATATATATATATACACACACACACACATAGATACATGTGTATATATTTTTCGTCTTAAAAAAGCTAAATAGCTAATTTAAATCTTATTTTTTGATCTCTAAATTGGAATGTTTGTTCTATTTTGATCTCAAAATGTTTAAATTAAGTTCTATTTTGATCTTTAAACTCTCAAAATATTTAGTTTTAGAACTTTTAAGATTAACAACTCTTTTATAATTCTTACCTTTATTTTTCCTTTTAACAAATTGATAAAATATAACTTCAAATATCTATACAAATAATTAATATATTAATAGGAGCATGGTGGGAGACTCCCAAATTAGAAACAAATATCACGTAACTGATCAATATTAGGTGGAAAAATTATATTCCCGAAGATCAAAACTATTACTTTTGGAAGTTTAGGACACACGTTTAAAAGTTCTGAGATTAAAATGAACAATGAGAATGAATAAGACCTTGTTTTGATGTCATTTCAAACATCTTAAATTATCAATGAATCCAAAGGCTTGTTACTGGAGTAGTGTAAATTTAATCATTTATAGATATCTTAACATTGTATTGCAGGATTTGAGATTAGCGACTACTCTAGTGAGGCCGCAGCCAATGTTCGATCTGATAAAATCGATGGAGGAGCTGGATCTGACCCGAGACCATTACGGGTCGGTCAAACGGGCCTTCATCATATCCGAGGACGACGAGATGGCCCTCGAGATCATGGTGTGGGCCATGCTCGTCTTGAACAAGCCGGATCGTGTGGCTGAGATCAAAGGATCAGATCACATGGTGATGGCTTCTAAGCCGCTTGATCTTGCCCAACAACTCTCCACCATCGCTCTAGAATTTGCTTCTTCCACTTCTATCTAA

mRNA sequence

ATGAAGAAGAACATGGGAAGAACACAGAAGCAATATTTGCATTTGCATAATCTGCTGCCATTTTTGCTTCTCCTCATTCTCCTCTCTCTCTCTTCTCCGACCCAGAGCTCGAGTCTCGGACTCCATAGTCAACCTCAGTCCTCGTCGAAAAAACTACACTTCGTGCTCGTCCATGGCGCCTGTCTCGGCGCCTGGTCGTGGTACAAGGTCACCACATACCTGCAATTGGCTGGCCACAATGTGACTGCCCTTGACATGGCTGGCGCCGGAATCAACCCGACGCAGCCCAAGTCGCTGTCTTCGCTCACCGATTACTATGAGCCATTGTTGAGGTTCATGGACGCGCTGCCCTCTGATGAGAAGGTCGTCCTGGTCGGGCACAGTCTCGGCGGGCTTGGAATCTCAATGGCCATGGAGGAGTTCCCCGAGAAGATCTCTGTTGCCATTTTTGTCACTGCAGCCATGCCTGGTCCAATCTCCAATTTAGAGGAAAAGAAGAAGATACTTGAAATGTATGGCACTTTCAACAAAAAGGATGAAGAACGATCCAAAGTTTCTGATGCAAAACTTTTTATTTTCACCGAAGAACAATTAGCAACCAAACTTTTCCCGCTCTCCCCGCCTGAGGATTTGAGATTAGCGACTACTCTAGTGAGGCCGCAGCCAATGTTCGATCTGATAAAATCGATGGAGGAGCTGGATCTGACCCGAGACCATTACGGGTCGGTCAAACGGGCCTTCATCATATCCGAGGACGACGAGATGGCCCTCGAGATCATGGTGTGGGCCATGCTCGTCTTGAACAAGCCGGATCGTGTGGCTGAGATCAAAGGATCAGATCACATGGTGATGGCTTCTAAGCCGCTTGATCTTGCCCAACAACTCTCCACCATCGCTCTAGAATTTGCTTCTTCCACTTCTATCTAA

Coding sequence (CDS)

ATGAAGAAGAACATGGGAAGAACACAGAAGCAATATTTGCATTTGCATAATCTGCTGCCATTTTTGCTTCTCCTCATTCTCCTCTCTCTCTCTTCTCCGACCCAGAGCTCGAGTCTCGGACTCCATAGTCAACCTCAGTCCTCGTCGAAAAAACTACACTTCGTGCTCGTCCATGGCGCCTGTCTCGGCGCCTGGTCGTGGTACAAGGTCACCACATACCTGCAATTGGCTGGCCACAATGTGACTGCCCTTGACATGGCTGGCGCCGGAATCAACCCGACGCAGCCCAAGTCGCTGTCTTCGCTCACCGATTACTATGAGCCATTGTTGAGGTTCATGGACGCGCTGCCCTCTGATGAGAAGGTCGTCCTGGTCGGGCACAGTCTCGGCGGGCTTGGAATCTCAATGGCCATGGAGGAGTTCCCCGAGAAGATCTCTGTTGCCATTTTTGTCACTGCAGCCATGCCTGGTCCAATCTCCAATTTAGAGGAAAAGAAGAAGATACTTGAAATGTATGGCACTTTCAACAAAAAGGATGAAGAACGATCCAAAGTTTCTGATGCAAAACTTTTTATTTTCACCGAAGAACAATTAGCAACCAAACTTTTCCCGCTCTCCCCGCCTGAGGATTTGAGATTAGCGACTACTCTAGTGAGGCCGCAGCCAATGTTCGATCTGATAAAATCGATGGAGGAGCTGGATCTGACCCGAGACCATTACGGGTCGGTCAAACGGGCCTTCATCATATCCGAGGACGACGAGATGGCCCTCGAGATCATGGTGTGGGCCATGCTCGTCTTGAACAAGCCGGATCGTGTGGCTGAGATCAAAGGATCAGATCACATGGTGATGGCTTCTAAGCCGCTTGATCTTGCCCAACAACTCTCCACCATCGCTCTAGAATTTGCTTCTTCCACTTCTATCTAA

Protein sequence

MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTSI

Homology

[Close]

BLAST of Moc04g06330 vs. NCBI nr
Match: XP_022136055.1 (methylesterase 3-like [Momordica charantia])

HSP 1 Score: 597.4 bits (1539), Expect = 6.9e-167
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGA 60
           MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGA
Sbjct: 1   MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGA 60

Query: 61  CLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDE 120
           CLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDE
Sbjct: 61  CLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDE 120

Query: 121 KVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDE 180
           KVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDE
Sbjct: 121 KVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDE 180

Query: 181 ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHY 240
           ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHY
Sbjct: 181 ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHY 240

Query: 241 GSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIAL 300
           GSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIAL
Sbjct: 241 GSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIAL 300

Query: 301 EFASSTSI 309
           EFASSTSI
Sbjct: 301 EFASSTSI 308

[Close]

BLAST of Moc04g06330 vs. NCBI nr
Match: XP_038887398.1 (probable esterase PIR7A [Benincasa hispida])

HSP 1 Score: 392.9 bits (1008), Expect = 2.6e-105
Identity = 209/296 (70.61%), Postives = 242/296 (81.76%), Query Frame = 0


Query: 15  LHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYL 74
           +  LL    LLI L   S TQSSS+ LH   Q S  K H VLVHGACLGAWSWYKVTT L
Sbjct: 1   MQKLLLLFFLLIFLCFCSQTQSSSV-LHDDSQGS--KSHIVLVHGACLGAWSWYKVTTLL 60

Query: 75  QLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGI 134
           Q AGHNVTALDMA +GINPTQ +SL+SLT+YY+PLL FM+AL  DEK+VLVGHSLGGLGI
Sbjct: 61  QTAGHNVTALDMAASGINPTQAESLTSLTEYYQPLLSFMEALAPDEKIVLVGHSLGGLGI 120

Query: 135 SMAMEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVSDAKLF 194
           SMAME FPEKISVAIFV+AAMPGPI   S++EE+KKILEMY  F    EE+SK S+  LF
Sbjct: 121 SMAMERFPEKISVAIFVSAAMPGPILGLSSIEEQKKILEMYSAF--YTEEKSKASNPDLF 180

Query: 195 IFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISE 254
           +F++E L TKLFPLSPPEDL LATTLVRPQ MF L++SM+EL L+++ YGSVKRAFIISE
Sbjct: 181 LFSQEDLETKLFPLSPPEDLTLATTLVRPQAMFGLLESMKELQLSQEKYGSVKRAFIISE 240

Query: 255 DDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
            D+M  +IMVWAML+ NKPDRVAE+KGSDHMVM SKPL+L QQLSTIA +F+SS++
Sbjct: 241 KDQMTTKIMVWAMLIFNKPDRVAEVKGSDHMVMTSKPLELTQQLSTIAQDFSSSST 291

[Close]

BLAST of Moc04g06330 vs. NCBI nr
Match: KAG6588637.1 (Methylesterase 8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 387.9 bits (995), Expect = 8.2e-104
Identity = 208/296 (70.27%), Postives = 239/296 (80.74%), Query Frame = 0


Query: 15  LHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYL 74
           +  LL   L+LIL+  SSPT+SS        QS S   HFVLVHGACLGAWSWYKV T L
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSSD-------QSESTYSHFVLVHGACLGAWSWYKVITLL 60

Query: 75  QLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGI 134
           Q AGH VTALDMA AGIN TQP+SL+SLT+Y+EPLL FM+ALPSDE++VLVGHSLGGLGI
Sbjct: 61  QTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGI 120

Query: 135 SMAMEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVSDAKLF 194
           SMAME FPEK+SVAIFVTAAMPGPI     +EE  KILEM   F    EE SK SD+KLF
Sbjct: 121 SMAMERFPEKVSVAIFVTAAMPGPIVGSFTVEELMKILEMLRAF--YSEEDSKASDSKLF 180

Query: 195 IFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISE 254
           +F++E+L TKLFPLSPP+D+ LATTL RPQ MF +++SM+EL LTRD YGSVKRAFIISE
Sbjct: 181 LFSQEELKTKLFPLSPPQDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISE 240

Query: 255 DDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
            DEM  +IMVWAML  NKPDR AE+KGSDHMVMASKPL+LAQQL+TIA +FASS++
Sbjct: 241 KDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSST 287

[Close]

BLAST of Moc04g06330 vs. NCBI nr
Match: XP_022931632.1 (methylesterase 2-like [Cucurbita moschata])

HSP 1 Score: 386.3 bits (991), Expect = 2.4e-103
Identity = 207/296 (69.93%), Postives = 238/296 (80.41%), Query Frame = 0


Query: 15  LHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYL 74
           +  LL   L+LIL+  SSPT+SS        QS S   HFVLVHGACLGAWSWYKV T L
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSSD-------QSESTYSHFVLVHGACLGAWSWYKVITLL 60

Query: 75  QLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGI 134
           Q AGH VTALDMA AGIN TQP+SL+SLT+Y+EPLL FM+ALPSDE++VLVGHSLGGLGI
Sbjct: 61  QTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGI 120

Query: 135 SMAMEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVSDAKLF 194
           SMAME FPEK+SVAIFVTAAMPGPI     +EE  KILEM   F    EE SK SD+ LF
Sbjct: 121 SMAMESFPEKVSVAIFVTAAMPGPIVGSFTIEELMKILEMLRAF--YSEEDSKASDSNLF 180

Query: 195 IFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISE 254
           +F++E+L TKLFPLSPP+D+ LATTL RPQ MF +++SM+EL LTRD YGSVKRAFIISE
Sbjct: 181 LFSQEELKTKLFPLSPPQDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISE 240

Query: 255 DDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
            DEM  +IMVWAML  NKPDR AE+KGSDHMVMASKPL+LAQQL+TIA +FASS++
Sbjct: 241 KDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSST 287

[Close]

BLAST of Moc04g06330 vs. NCBI nr
Match: XP_008454899.1 (PREDICTED: methylesterase 2-like [Cucumis melo])

HSP 1 Score: 383.6 bits (984), Expect = 1.6e-102
Identity = 200/294 (68.03%), Postives = 243/294 (82.65%), Query Frame = 0


Query: 18  LLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYLQLA 77
           LLP   LLI LS S+ TQ+S   LH    S S K HFVLVHGACLGAWSWYKVTT+LQ A
Sbjct: 5   LLPLFFLLIFLSFSTQTQTSFDLLHHH-YSQSTKSHFVLVHGACLGAWSWYKVTTFLQTA 64

Query: 78  GHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGISMA 137
           GHNVTALDMA AGI+PTQP+SL ++TDY++PLL FM+AL +DEK+VLVGHSLGGLGISMA
Sbjct: 65  GHNVTALDMAAAGIDPTQPQSLKTITDYFQPLLNFMEALQADEKIVLVGHSLGGLGISMA 124

Query: 138 MEEFPEKISVAIFVTAAMPGPIS---NLEEKKKILEMYGTFNKKDEERSKVSD-AKLFIF 197
           ME FPEKISVAIFVTAAMPGPI    ++EE++K+L MYG F  +++ +SK SD + LF+F
Sbjct: 125 MERFPEKISVAIFVTAAMPGPIPWLFSMEEQRKVLGMYGGFYTEEDFKSKGSDNSNLFMF 184

Query: 198 TEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISEDD 257
           +EE+L TKLFPLSPPEDL LA TLVRPQ MF L +S++EL L++++YGSVKRAFIISE D
Sbjct: 185 SEEELETKLFPLSPPEDLTLAKTLVRPQAMFGLFESVKELKLSKENYGSVKRAFIISEKD 244

Query: 258 EMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
           +MA +IMVWAM++ N+P  V E+ GSDHMVM SKPL+LA+QLSTIA +FA++ S
Sbjct: 245 KMATKIMVWAMIISNRPHHVGEVHGSDHMVMTSKPLELARQLSTIAQDFAAAAS 297

[Close]

BLAST of Moc04g06330 vs. ExPASy Swiss-Prot
Match: O80477 (Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 1.1e-45
Identity = 115/267 (43.07%), Postives = 156/267 (58.43%), Query Frame = 0


Query: 50  KKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQP-KSLSSLTDYYEP 109
           +K H VLVHGAC GAW WYKV   L+ +GH VTA+D+A +GI+ T+    +S+   Y EP
Sbjct: 6   RKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEP 65

Query: 110 LLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMP----------GP 169
           L++ M +LP DEKVVLVGHSLGGL ++MAM+ FP KISV++FVTA MP            
Sbjct: 66  LMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDK 125

Query: 170 ISNLEEKKKILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLV 229
           +     +++ L+   T  K D        ++ +IF  E +A  L+ LSP +DL LA  LV
Sbjct: 126 LRKETSREEWLDTVFTSEKPD------FPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLV 185

Query: 230 RPQPMFDLIKSM-EELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIK 289
           R  P+  + K M E    + + YGSV R FI+   D ++ E    +M+    P  V EIK
Sbjct: 186 RANPL--IKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIK 245

Query: 290 GSDHMVMASKPLDLAQQLSTIALEFAS 305
            +DHM M SKP    QQL  + LE A+
Sbjct: 246 DADHMPMFSKP----QQLCALLLEIAN 260

[Close]

BLAST of Moc04g06330 vs. ExPASy Swiss-Prot
Match: Q9SE93 (Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 6.9e-45
Identity = 110/267 (41.20%), Postives = 157/267 (58.80%), Query Frame = 0


Query: 41  LHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLS 100
           +HS   +  +K HFVLVHG CLGAW WYK+   L+ AGH VTA+D++ AGINP +   + 
Sbjct: 1   MHSAANAKQQK-HFVLVHGGCLGAWIWYKLKPLLESAGHKVTAVDLSAAGINPRRLDEIH 60

Query: 101 SLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPIS 160
           +  DY EPL+  M ++P DEKVVL+GHS GG+ + +AME +PEKISVA+F++A MP P  
Sbjct: 61  TFRDYSEPLMEVMASIPPDEKVVLLGHSFGGMSLGLAMETYPEKISVAVFMSAMMPDPNH 120

Query: 161 NLEEK-KKILEMYGTFNKKDEERSKVSDAK----LFIFTEEQLATKLFPLSPPEDLRLAT 220
           +L    +K  E        D + S   + +      I   + +A K+F     EDL LA 
Sbjct: 121 SLTYPFEKYNEKCPADMMLDSQFSTYGNPENPGMSMILGPQFMALKMFQNCSVEDLELAK 180

Query: 221 TLVRPQPMF--DLIKSMEELDLTRDHYGSVKRAFII-SEDDEMALEIMVWAMLVLNKPDR 280
            L RP  +F  DL K+ +    + + YGSVKRA+I  +ED    +E   W +  +   D+
Sbjct: 181 MLTRPGSLFFQDLAKAKK---FSTERYGSVKRAYIFCNEDKSFPVEFQKWFVESVG-ADK 240

Query: 281 VAEIKGSDHMVMASKPLDLAQQLSTIA 300
           V EIK +DHM M S+P ++ + L  I+
Sbjct: 241 VKEIKEADHMGMLSQPREVCKCLLDIS 262

[Close]

BLAST of Moc04g06330 vs. ExPASy Swiss-Prot
Match: O80475 (Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.4e-44
Identity = 106/254 (41.73%), Postives = 151/254 (59.45%), Query Frame = 0


Query: 51  KLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLL 110
           K HFVLVHG+CLGAW WYKV   L+ +GH VTALD+A  GI+      +S+   Y EPL+
Sbjct: 25  KQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLI 84

Query: 111 RFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILE 170
           + M +LP+DEKVVLVGHS GGL +++AM++FP+KISV++FVT+ MP           +LE
Sbjct: 85  QLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMP---DTKNSPSFVLE 144

Query: 171 MY-GTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKS 230
            +  T   +D   S++      +F+ E    ++  LSP EDL L   L RP  +F L   
Sbjct: 145 KFASTMTPEDWMGSELE--PYVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLF-LNDL 204

Query: 231 MEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPL 290
               + +   YGSV RA+I+S+DD    E     M+    P+ V E++G+DH+ +  KP 
Sbjct: 205 SRMKNFSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKPQ 264

Query: 291 DLAQQLSTIALEFA 304
            L+  L  IA +F+
Sbjct: 265 LLSDHLLAIADKFS 272

[Close]

BLAST of Moc04g06330 vs. ExPASy Swiss-Prot
Match: Q6RYA0 (Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.7e-43
Identity = 106/263 (40.30%), Postives = 150/263 (57.03%), Query Frame = 0


Query: 53  HFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRF 112
           HFVLVHGAC G WSWYK+   L+ AGH VTALD+A +G +  + + L +L DY  PL+  
Sbjct: 6   HFVLVHGACHGGWSWYKLKPLLEAAGHKVTALDLAASGTDLRKIEELRTLYDYTLPLMEL 65

Query: 113 MDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMY 172
           M++L +DEKV+LVGHSLGG+ + +AME++P+KI  A+F+ A MP  + N      +LE Y
Sbjct: 66  MESLSADEKVILVGHSLGGMNLGLAMEKYPQKIYAAVFLAAFMPDSVHN---SSFVLEQY 125

Query: 173 GTFNKKDE--------ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMF 232
                 +           S         F  + LA KL+ L  PEDL LA++LVRP  +F
Sbjct: 126 NERTPAENWLDTQFLPYGSPEEPLTSMFFGPKFLAHKLYQLCSPEDLALASSLVRPSSLF 185

Query: 233 DLIKSMEELD----LTRDHYGSVKRAFII-SEDDEMALEIMVWAMLVLNKPDRVAEIKGS 292
                ME+L      T + +GSVKR +I+ +ED  +  E   W +  +   + + EIKG+
Sbjct: 186 -----MEDLSKAKYFTDERFGSVKRVYIVCTEDKGIPEEFQRWQIDNIGVTEAI-EIKGA 245

Query: 293 DHMVMASKPLDLAQQLSTIALEF 303
           DHM M  +P  L   L  IA ++
Sbjct: 246 DHMAMLCEPQKLCASLLEIAHKY 259

[Close]

BLAST of Moc04g06330 vs. ExPASy Swiss-Prot
Match: O80476 (Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1)

HSP 1 Score: 177.6 bits (449), Expect = 2.2e-43
Identity = 108/259 (41.70%), Postives = 153/259 (59.07%), Query Frame = 0


Query: 50  KKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQP-KSLSSLTDYYEP 109
           +K HFVLVHGAC GAW WYKV   L+  GH VTALD+A +GI+ T+    +S+   Y EP
Sbjct: 6   RKQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEP 65

Query: 110 LLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPG---PISNLEEK 169
           L++ M +LP+DEKVVLVGHS GGL +++AM++FP+KISV++FVTA MP      S +EEK
Sbjct: 66  LMQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEK 125

Query: 170 -KKILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMF 229
               +   G    + E     +      F+ + +  +L+ LSP EDL L   L RP  +F
Sbjct: 126 FASSMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSLF 185

Query: 230 -DLIKSMEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMV 289
            + +  ME  + +   YGSV RA+I+ ++D +  E     M+     + V E++ +DHM 
Sbjct: 186 INELSKME--NFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMP 245

Query: 290 MASKPLDLAQQLSTIALEF 303
           M  KP  L+  L  IA  F
Sbjct: 246 MFCKPQLLSDHLLAIADNF 262

[Close]

BLAST of Moc04g06330 vs. ExPASy TrEMBL
Match: A0A6J1C2S8 (methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111007844 PE=4 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.3e-167
Identity = 308/308 (100.00%), Postives = 308/308 (100.00%), Query Frame = 0


Query: 1   MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGA 60
           MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGA
Sbjct: 1   MKKNMGRTQKQYLHLHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGA 60

Query: 61  CLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDE 120
           CLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDE
Sbjct: 61  CLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDE 120

Query: 121 KVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDE 180
           KVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDE
Sbjct: 121 KVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILEMYGTFNKKDE 180

Query: 181 ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHY 240
           ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHY
Sbjct: 181 ERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHY 240

Query: 241 GSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIAL 300
           GSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIAL
Sbjct: 241 GSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIAL 300

Query: 301 EFASSTSI 309
           EFASSTSI
Sbjct: 301 EFASSTSI 308

[Close]

BLAST of Moc04g06330 vs. ExPASy TrEMBL
Match: A0A6J1EU76 (methylesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437792 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 1.2e-103
Identity = 207/296 (69.93%), Postives = 238/296 (80.41%), Query Frame = 0


Query: 15  LHNLLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYL 74
           +  LL   L+LIL+  SSPT+SS        QS S   HFVLVHGACLGAWSWYKV T L
Sbjct: 1   MQKLLLSFLVLILVLFSSPTRSSD-------QSESTYSHFVLVHGACLGAWSWYKVITLL 60

Query: 75  QLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGI 134
           Q AGH VTALDMA AGIN TQP+SL+SLT+Y+EPLL FM+ALPSDE++VLVGHSLGGLGI
Sbjct: 61  QTAGHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGI 120

Query: 135 SMAMEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVSDAKLF 194
           SMAME FPEK+SVAIFVTAAMPGPI     +EE  KILEM   F    EE SK SD+ LF
Sbjct: 121 SMAMESFPEKVSVAIFVTAAMPGPIVGSFTIEELMKILEMLRAF--YSEEDSKASDSNLF 180

Query: 195 IFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISE 254
           +F++E+L TKLFPLSPP+D+ LATTL RPQ MF +++SM+EL LTRD YGSVKRAFIISE
Sbjct: 181 LFSQEELKTKLFPLSPPQDVALATTLERPQAMFGILESMKELRLTRDKYGSVKRAFIISE 240

Query: 255 DDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
            DEM  +IMVWAML  NKPDR AE+KGSDHMVMASKPL+LAQQL+TIA +FASS++
Sbjct: 241 KDEMTSKIMVWAMLTFNKPDRQAEVKGSDHMVMASKPLELAQQLTTIAQDFASSST 287

[Close]

BLAST of Moc04g06330 vs. ExPASy TrEMBL
Match: A0A1S3C0U9 (methylesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103495205 PE=4 SV=1)

HSP 1 Score: 383.6 bits (984), Expect = 7.5e-103
Identity = 200/294 (68.03%), Postives = 243/294 (82.65%), Query Frame = 0


Query: 18  LLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYLQLA 77
           LLP   LLI LS S+ TQ+S   LH    S S K HFVLVHGACLGAWSWYKVTT+LQ A
Sbjct: 5   LLPLFFLLIFLSFSTQTQTSFDLLHHH-YSQSTKSHFVLVHGACLGAWSWYKVTTFLQTA 64

Query: 78  GHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGISMA 137
           GHNVTALDMA AGI+PTQP+SL ++TDY++PLL FM+AL +DEK+VLVGHSLGGLGISMA
Sbjct: 65  GHNVTALDMAAAGIDPTQPQSLKTITDYFQPLLNFMEALQADEKIVLVGHSLGGLGISMA 124

Query: 138 MEEFPEKISVAIFVTAAMPGPIS---NLEEKKKILEMYGTFNKKDEERSKVSD-AKLFIF 197
           ME FPEKISVAIFVTAAMPGPI    ++EE++K+L MYG F  +++ +SK SD + LF+F
Sbjct: 125 MERFPEKISVAIFVTAAMPGPIPWLFSMEEQRKVLGMYGGFYTEEDFKSKGSDNSNLFMF 184

Query: 198 TEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISEDD 257
           +EE+L TKLFPLSPPEDL LA TLVRPQ MF L +S++EL L++++YGSVKRAFIISE D
Sbjct: 185 SEEELETKLFPLSPPEDLTLAKTLVRPQAMFGLFESVKELKLSKENYGSVKRAFIISEKD 244

Query: 258 EMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
           +MA +IMVWAM++ N+P  V E+ GSDHMVM SKPL+LA+QLSTIA +FA++ S
Sbjct: 245 KMATKIMVWAMIISNRPHHVGEVHGSDHMVMTSKPLELARQLSTIAQDFAAAAS 297

[Close]

BLAST of Moc04g06330 vs. ExPASy TrEMBL
Match: A0A6J1JNJ9 (probable esterase PIR7A OS=Cucurbita maxima OX=3661 GN=LOC111486088 PE=4 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 1.3e-102
Identity = 208/293 (70.99%), Postives = 236/293 (80.55%), Query Frame = 0


Query: 18  LLPFLLLLILLSLSSPTQSSSLGLHSQPQSSSKKLHFVLVHGACLGAWSWYKVTTYLQLA 77
           LLPF L+LIL+S SSPT SS        QS S   HFVLVHGACLGAWSWYKV T LQ A
Sbjct: 14  LLPF-LVLILVSFSSPTWSSD-------QSESTYSHFVLVHGACLGAWSWYKVITLLQTA 73

Query: 78  GHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGLGISMA 137
           GH VTALDMA AGIN TQP+SL+SLT+Y+EPLL FM+ALPSDE++VLVGHSLGGLGISMA
Sbjct: 74  GHKVTALDMAAAGINQTQPESLASLTEYFEPLLSFMEALPSDERIVLVGHSLGGLGISMA 133

Query: 138 MEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVSDAKLFIFT 197
           ME FPEK+SVAIFVTAAMPGPI     +E+  KIL+M   F    EE SK S++ LF+F+
Sbjct: 134 MERFPEKVSVAIFVTAAMPGPIVGSFTIEKLMKILQMLRAF--YSEEDSKASNSNLFLFS 193

Query: 198 EEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAFIISEDDE 257
           +E+L  KLFPLSPPEDL LATTLVRPQ MF +++SM+EL LTRD YGSVKRAFIISE DE
Sbjct: 194 QEELEAKLFPLSPPEDLTLATTLVRPQAMFGILESMKELRLTRDKYGSVKRAFIISEKDE 253

Query: 258 MALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
           M   IMVWAML  NKPDR A +KGSDHMVMASKPL LAQQL+TIA +FASS++
Sbjct: 254 MTSMIMVWAMLTFNKPDRQAVVKGSDHMVMASKPLQLAQQLTTIAQDFASSST 296

[Close]

BLAST of Moc04g06330 vs. ExPASy TrEMBL
Match: A0A0A0K4V8 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074970 PE=4 SV=1)

HSP 1 Score: 373.6 bits (958), Expect = 7.8e-100
Identity = 198/300 (66.00%), Postives = 242/300 (80.67%), Query Frame = 0


Query: 18  LLPFLLLLILLSLSSPTQSSSLGLHSQ-----PQSSSKKLHFVLVHGACLGAWSWYKVTT 77
           LLPF  LLI LS S+ TQ+S L  H         S S K HFVLVHGACLGAWSWY+VTT
Sbjct: 5   LLPF-FLLIFLSFSTQTQTSFLFHHGHHDNDGHDSQSNKSHFVLVHGACLGAWSWYQVTT 64

Query: 78  YLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRFMDALPSDEKVVLVGHSLGGL 137
           +LQ AGH VTA+DMA AGI+PTQP+SL+SLTDY++PLL F +AL +D+K+VLVGHSLGGL
Sbjct: 65  FLQTAGHKVTAVDMAAAGIDPTQPESLTSLTDYFQPLLNFTEALQADDKIVLVGHSLGGL 124

Query: 138 GISMAMEEFPEKISVAIFVTAAMPGPI---SNLEEKKKILEMYGTFNKKDEERSKVS--D 197
           GISMAME FPEKISVAIFVTAAMPGPI    ++EE+K  L MY  F+K D+ +S+VS   
Sbjct: 125 GISMAMERFPEKISVAIFVTAAMPGPIIGFQSIEEQKTTLGMYKAFHKGDDTKSEVSKKP 184

Query: 198 AKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKSMEELDLTRDHYGSVKRAF 257
            +LF+F+EE+L TKLFPLSPP+DL LA TLVRPQ MF L++SM+EL L++++YGSVKRAF
Sbjct: 185 PRLFMFSEEELETKLFPLSPPQDLTLARTLVRPQAMFGLLESMKELRLSKENYGSVKRAF 244

Query: 258 IISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPLDLAQQLSTIALEFASSTS 308
           IIS++D+M  + MVWAML+LNKPDRV E+ GSDHMVM SKPL+LAQ L TIA ++A+ +S
Sbjct: 245 IISQNDKMTSKFMVWAMLLLNKPDRVEEVHGSDHMVMTSKPLELAQLLGTIAQDYAAFSS 303

[Close]

BLAST of Moc04g06330 vs. TAIR 10
Match: AT2G23610.1 (methyl esterase 3 )

HSP 1 Score: 185.3 bits (469), Expect = 7.6e-47
Identity = 115/267 (43.07%), Postives = 156/267 (58.43%), Query Frame = 0


Query: 50  KKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQP-KSLSSLTDYYEP 109
           +K H VLVHGAC GAW WYKV   L+ +GH VTA+D+A +GI+ T+    +S+   Y EP
Sbjct: 6   RKQHVVLVHGACHGAWCWYKVKPQLEASGHRVTAVDLAASGIDMTRSITDISTCEQYSEP 65

Query: 110 LLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMP----------GP 169
           L++ M +LP DEKVVLVGHSLGGL ++MAM+ FP KISV++FVTA MP            
Sbjct: 66  LMQLMTSLPDDEKVVLVGHSLGGLSLAMAMDMFPTKISVSVFVTAMMPDTKHSPSFVWDK 125

Query: 170 ISNLEEKKKILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLV 229
           +     +++ L+   T  K D        ++ +IF  E +A  L+ LSP +DL LA  LV
Sbjct: 126 LRKETSREEWLDTVFTSEKPD------FPSEFWIFGPEFMAKNLYQLSPVQDLELAKMLV 185

Query: 230 RPQPMFDLIKSM-EELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIK 289
           R  P+  + K M E    + + YGSV R FI+   D ++ E    +M+    P  V EIK
Sbjct: 186 RANPL--IKKDMAERRSFSEEGYGSVTRIFIVCGKDLVSPEDYQRSMISNFPPKEVMEIK 245

Query: 290 GSDHMVMASKPLDLAQQLSTIALEFAS 305
            +DHM M SKP    QQL  + LE A+
Sbjct: 246 DADHMPMFSKP----QQLCALLLEIAN 260

[Close]

BLAST of Moc04g06330 vs. TAIR 10
Match: AT2G23590.1 (methyl esterase 8 )

HSP 1 Score: 180.3 bits (456), Expect = 2.4e-45
Identity = 106/254 (41.73%), Postives = 151/254 (59.45%), Query Frame = 0


Query: 51  KLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLL 110
           K HFVLVHG+CLGAW WYKV   L+ +GH VTALD+A  GI+      +S+   Y EPL+
Sbjct: 25  KQHFVLVHGSCLGAWCWYKVKPLLEASGHRVTALDLAACGIDTRSITDISTCEQYSEPLI 84

Query: 111 RFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPGPISNLEEKKKILE 170
           + M +LP+DEKVVLVGHS GGL +++AM++FP+KISV++FVT+ MP           +LE
Sbjct: 85  QLMTSLPNDEKVVLVGHSYGGLTLAIAMDKFPDKISVSVFVTSFMP---DTKNSPSFVLE 144

Query: 171 MY-GTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLIKS 230
            +  T   +D   S++      +F+ E    ++  LSP EDL L   L RP  +F L   
Sbjct: 145 KFASTMTPEDWMGSELE--PYVVFSAEFTKHRILQLSPIEDLELRLLLKRPGSLF-LNDL 204

Query: 231 MEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASKPL 290
               + +   YGSV RA+I+S+DD    E     M+    P+ V E++G+DH+ +  KP 
Sbjct: 205 SRMKNFSEKGYGSVPRAYIVSKDDHTISEEYQRWMIDNYPPNLVIEMEGTDHLPLFCKPQ 264

Query: 291 DLAQQLSTIALEFA 304
            L+  L  IA +F+
Sbjct: 265 LLSDHLLAIADKFS 272

[Close]

BLAST of Moc04g06330 vs. TAIR 10
Match: AT2G23600.1 (acetone-cyanohydrin lyase )

HSP 1 Score: 177.6 bits (449), Expect = 1.6e-44
Identity = 108/259 (41.70%), Postives = 153/259 (59.07%), Query Frame = 0


Query: 50  KKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQP-KSLSSLTDYYEP 109
           +K HFVLVHGAC GAW WYKV   L+  GH VTALD+A +GI+ T+    +S+   Y EP
Sbjct: 6   RKQHFVLVHGACHGAWCWYKVKPLLEALGHRVTALDLAASGIDTTRSITDISTCEQYSEP 65

Query: 110 LLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMPG---PISNLEEK 169
           L++ M +LP+DEKVVLVGHS GGL +++AM++FP+KISV++FVTA MP      S +EEK
Sbjct: 66  LMQLMTSLPNDEKVVLVGHSFGGLSLALAMDKFPDKISVSVFVTAFMPDTKHSPSFVEEK 125

Query: 170 -KKILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMF 229
               +   G    + E     +      F+ + +  +L+ LSP EDL L   L RP  +F
Sbjct: 126 FASSMTPEGWMGSELETYGSDNSGLSVFFSTDFMKHRLYQLSPVEDLELGLLLKRPSSLF 185

Query: 230 -DLIKSMEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMV 289
            + +  ME  + +   YGSV RA+I+ ++D +  E     M+     + V E++ +DHM 
Sbjct: 186 INELSKME--NFSEKGYGSVPRAYIVCKEDNIISEDHQRWMIHNYPANLVIEMEETDHMP 245

Query: 290 MASKPLDLAQQLSTIALEF 303
           M  KP  L+  L  IA  F
Sbjct: 246 MFCKPQLLSDHLLAIADNF 262

[Close]

BLAST of Moc04g06330 vs. TAIR 10
Match: AT2G23620.1 (methyl esterase 1 )

HSP 1 Score: 170.2 bits (430), Expect = 2.5e-42
Identity = 104/260 (40.00%), Postives = 147/260 (56.54%), Query Frame = 0


Query: 50  KKLHFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQP-KSLSSLTDYYEP 109
           +K HFVLVHG+C GAW WYKV   L+  GH VTA+D+A +GI+ T+    + +   Y EP
Sbjct: 6   RKQHFVLVHGSCHGAWCWYKVKPLLEAVGHRVTAVDLAASGIDTTRSITDIPTCEQYSEP 65

Query: 110 LLRFMDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMP----GPISNLEE 169
           L + + +LP+DEKVVLVGHS GGL +++AME+FPEKISVA+F+TA MP     P   L++
Sbjct: 66  LTKLLTSLPNDEKVVLVGHSFGGLNLAIAMEKFPEKISVAVFLTAFMPDTEHSPSFVLDK 125

Query: 170 KKKILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMF 229
               +        + E     +      F+ + +   L+ LSP EDL L   L+RP  +F
Sbjct: 126 FGSNMPQEAWMGTEFEPYGSDNSGLSMFFSPDFMKLGLYQLSPVEDLELGLLLMRPGSLF 185

Query: 230 --DLIKSMEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHM 289
             DL K     D   + YGSV R FI+ ++D+   E     M+     + V E++ +DHM
Sbjct: 186 INDLSKMKNFSD---EGYGSVPRVFIVCKEDKAIPEERQRWMIDNFPVNLVMEMEETDHM 245

Query: 290 VMASKPLDLAQQLSTIALEF 303
            M  KP  L+     IA +F
Sbjct: 246 PMFCKPQQLSDYFLKIADKF 262

[Close]

BLAST of Moc04g06330 vs. TAIR 10
Match: AT3G50440.1 (methyl esterase 10 )

HSP 1 Score: 170.2 bits (430), Expect = 2.5e-42
Identity = 99/255 (38.82%), Postives = 147/255 (57.65%), Query Frame = 0


Query: 53  HFVLVHGACLGAWSWYKVTTYLQLAGHNVTALDMAGAGINPTQPKSLSSLTDYYEPLLRF 112
           HFV VHG+C GAW W+K+   L+L GH VTA+D+ G+G++  Q   +  ++ Y EPL+ F
Sbjct: 34  HFVFVHGSCHGAWCWFKLAAKLKLDGHRVTAIDLGGSGVDTRQLHEVRLVSAYLEPLMSF 93

Query: 113 MDALPSDEKVVLVGHSLGGLGISMAMEEFPEKISVAIFVTAAMP---GPISNL--EEKKK 172
           M++LP +EKVVLVGHS GG+G S+AME FP K+SV IF++A MP    P + L  E   +
Sbjct: 94  MESLPENEKVVLVGHSYGGIGTSLAMERFPTKVSVGIFLSAYMPHHDSPPAVLIQEYFTR 153

Query: 173 ILEMYGTFNKKDEERSKVSDAKLFIFTEEQLATKLFPLSPPEDLRLATTLVRPQPMFDLI 232
           + E +    +   E          +F    L  K +     EDL LA  L++P  ++   
Sbjct: 154 LPEGFAMDCEFTFEEGLEHPPSSVLFGTSFLKEKAYSNCQLEDLELAMALMKPSWLYTKE 213

Query: 233 KSMEELDLTRDHYGSVKRAFIISEDDEMALEIMVWAMLVLNKPDRVAEIKGSDHMVMASK 292
              E+L +T++ YGS KR FI+ E D +  E +   M+   +P  V  I+ + HM M +K
Sbjct: 214 MGGEDL-ITKERYGSGKRVFIVCEGDNVVPEEIQKWMISNYEPHEVKRIEEAGHMAMLTK 273

Query: 293 PLDLAQQLSTIALEF 303
           P +L+Q L  IA ++
Sbjct: 274 PHELSQLLQEIAAKY 287

The following BLAST results are available for this feature:

BLAST of Moc04g06330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136055.1	6.9e-167	100.00	methylesterase 3-like [Momordica charantia]	[more]
XP_038887398.1	2.6e-105	70.61	probable esterase PIR7A [Benincasa hispida]	[more]
KAG6588637.1	8.2e-104	70.27	Methylesterase 8, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022931632.1	2.4e-103	69.93	methylesterase 2-like [Cucurbita moschata]	[more]
XP_008454899.1	1.6e-102	68.03	PREDICTED: methylesterase 2-like [Cucumis melo]	[more]

BLAST of Moc04g06330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80477	1.1e-45	43.07	Methylesterase 3 OS=Arabidopsis thaliana OX=3702 GN=MES3 PE=2 SV=1	[more]
Q9SE93	6.9e-45	41.20	Polyneuridine-aldehyde esterase OS=Rauvolfia serpentina OX=4060 GN=PNAE PE=1 SV=...	[more]
O80475	3.4e-44	41.73	Methylesterase 8 OS=Arabidopsis thaliana OX=3702 GN=MES8 PE=3 SV=1	[more]
Q6RYA0	1.7e-43	40.30	Salicylic acid-binding protein 2 OS=Nicotiana tabacum OX=4097 GN=SABP2 PE=1 SV=1	[more]
O80476	2.2e-43	41.70	Methylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=MES2 PE=1 SV=1	[more]

BLAST of Moc04g06330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C2S8	3.3e-167	100.00	methylesterase 3-like OS=Momordica charantia OX=3673 GN=LOC111007844 PE=4 SV=1	[more]
A0A6J1EU76	1.2e-103	69.93	methylesterase 2-like OS=Cucurbita moschata OX=3662 GN=LOC111437792 PE=4 SV=1	[more]
A0A1S3C0U9	7.5e-103	68.03	methylesterase 2-like OS=Cucumis melo OX=3656 GN=LOC103495205 PE=4 SV=1	[more]
A0A6J1JNJ9	1.3e-102	70.99	probable esterase PIR7A OS=Cucurbita maxima OX=3661 GN=LOC111486088 PE=4 SV=1	[more]
A0A0A0K4V8	7.8e-100	66.00	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G074...	[more]

BLAST of Moc04g06330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G23610.1	7.6e-47	43.07	methyl esterase 3	[more]
AT2G23590.1	2.4e-45	41.73	methyl esterase 8	[more]
AT2G23600.1	1.6e-44	41.70	acetone-cyanohydrin lyase	[more]
AT2G23620.1	2.5e-42	40.00	methyl esterase 1	[more]
AT3G50440.1	2.5e-42	38.82	methyl esterase 10	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 46..303 e-value: 2.7E-57 score: 196.5
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 49..295
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF12697	Abhydrolase_6	coord: 54..292 e-value: 2.0E-17 score: 64.5
None	No IPR available	PANTHER	PTHR10992	METHYLESTERASE FAMILY MEMBER	coord: 40..300
None	No IPR available	PANTHER	PTHR10992:SF1002	ESTERASE PIR7A-RELATED	coord: 40..300

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g06330.1	Moc04g06330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009694	jasmonic acid metabolic process
biological_process	GO:0009696	salicylic acid metabolic process
molecular_function	GO:0080030	methyl indole-3-acetate esterase activity
molecular_function	GO:0080032	methyl jasmonate esterase activity
molecular_function	GO:0080031	methyl salicylate esterase activity