Moc04g06190 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g06190
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionallene oxide synthase 3-like
Locationchr4: 4247714 .. 4249432 (-)
RNA-Seq ExpressionMoc04g06190
SyntenyMoc04g06190
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTTCCTCTTCTTCTTCCTCCGAACGGCCACTGAAACCCATTCCCGGCGGCTACGGCTTCTCCATTTTTGGGCCCATCAAGGATCGCTACGACTACTTCTACATCCAAGGCCGAGAAAAGTTCTTCCGAACCCGGATGAGTAAATACGGGTCAACCGTTTTCCGGGTCAACATGCCGCCAGGTCCCTTCATCTCCTCCGATTCCAGAGTCATCGTCCTCCTCGACGCTGTCAGTTTTCCGATCCTCTTCGACACCACCAAGGTCGAGAAACGCAACGTTCTTGACGGAACTTACATGCCGTCGGTGGCCTTCACCGGCAATATTCGCACGTGCGCTTTTCTTGACCCGTCGGAGCCAGAGCACTCTGCTCTCAAGCGCCTGTTCCTCTCTTTTCTCGCCTCTCGCCACGACAAGTTTGTTCCCCTGTTCCGGAGCTCCTTGTCGGATATGTTCGATAAGCTGGACGGGAAATTGGAGGAAAAGAACGCCGCTGCCGCCGATTTCAATCCGATGAGTGACGCGGCGGCGTTTGACTTCATCTTCCGGCTGCTCACCGATCGGAGCCCCGACGAGAAATTGGGATCCGAGGGGCCGGGTCTTGTCGATAAATGGCTGGCCATGCAGCTCGCGCCGTTGGCTTCTGCCGGCCTCCCCAAATTCCTCAGCTGCTTCGAAGATCTCGTCATCCACACTTTTCCATTGCCCTTCTTCCCCGTCAAAAGTTCCTACAGGTGAGAATGATTAGTACATGAAATTCAATTTGTAAGTAATAATCGTATTCATTTGATTGAAACCGTATCAATATTTACATCTCAAGACCAATTAGTAGCAGCCCATTAGGATCTTTAACTATGAATTCTATTTTTAGACTGAATACTTGTTGTATGATACTATATCAATATTCATATTCATAGGTTATATCTCAAATTAATTAACAACGAGAAGAGCATTGAGGTCTATTATAAACTATTTCGAGATCTTTTATCGTGGAGTTGTGATTTCAGGAAGCTGTACGATGCGTTTTACGCATCCTCTGTTTCGTTTCTGGACGAAGCAGAGAGACAGGGGATAGACAGAGAGGAAGCCTGCCACAATTTAGTGTTCCTGGCCGGATTCAACGCGTACGCCGGAATGAAGGTGATGCTTCCGATTCTGCTCAATTGGGTCGGGTCCGCCGGCGAAGGGCTCCACCGGGAGCTCGCCGAGGAGGTCAGAACCGTCGTCAAGGAAGAAGGGGGCTTGACTTTGGGCGCAATAGAGAAGATGAGGCTGCTGAAATCCGTCGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAGTACGCGCGGGCGAAAGAAGATATCGTGATCCAAAGCCACGATTTCTCCTACAAGATAAAGAAAGGGGAGATGATTTTTGGGTATCAGCCGATCGCGACCAGAGACCCGAAGGTTTTCGAGAATCCGGAGAAGTTCGTCGGCGACAGGTTCGTCGGAGAGAAAGGGGAGAAGCTTCTGAATTATGTTTACTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGCGAAGGATCTGGTGGTGCTGGTGGCCAGGCTAATTCTGGTGGAGCTCTTCCTCCGTTACGACTCGTTTACCGTCGAGGGGACTCAGTCGTCGCTCGGATGGACAGTGAAGATCAAATCGTTGACCAAATCGACCGCTAAGGTTTGA

mRNA sequence

ATGAGTTCCTCTTCTTCTTCCTCCGAACGGCCACTGAAACCCATTCCCGGCGGCTACGGCTTCTCCATTTTTGGGCCCATCAAGGATCGCTACGACTACTTCTACATCCAAGGCCGAGAAAAGTTCTTCCGAACCCGGATGAGTAAATACGGGTCAACCGTTTTCCGGGTCAACATGCCGCCAGGTCCCTTCATCTCCTCCGATTCCAGAGTCATCGTCCTCCTCGACGCTGTCAGTTTTCCGATCCTCTTCGACACCACCAAGGTCGAGAAACGCAACGTTCTTGACGGAACTTACATGCCGTCGGTGGCCTTCACCGGCAATATTCGCACGTGCGCTTTTCTTGACCCGTCGGAGCCAGAGCACTCTGCTCTCAAGCGCCTGTTCCTCTCTTTTCTCGCCTCTCGCCACGACAAGTTTGTTCCCCTGTTCCGGAGCTCCTTGTCGGATATGTTCGATAAGCTGGACGGGAAATTGGAGGAAAAGAACGCCGCTGCCGCCGATTTCAATCCGATGAGTGACGCGGCGGCGTTTGACTTCATCTTCCGGCTGCTCACCGATCGGAGCCCCGACGAGAAATTGGGATCCGAGGGGCCGGGTCTTGTCGATAAATGGCTGGCCATGCAGCTCGCGCCGTTGGCTTCTGCCGGCCTCCCCAAATTCCTCAGCTGCTTCGAAGATCTCGTCATCCACACTTTTCCATTGCCCTTCTTCCCCGTCAAAAGTTCCTACAGGAAGCTGTACGATGCGTTTTACGCATCCTCTGTTTCGTTTCTGGACGAAGCAGAGAGACAGGGGATAGACAGAGAGGAAGCCTGCCACAATTTAGTGTTCCTGGCCGGATTCAACGCGTACGCCGGAATGAAGGTGATGCTTCCGATTCTGCTCAATTGGGTCGGGTCCGCCGGCGAAGGGCTCCACCGGGAGCTCGCCGAGGAGGTCAGAACCGTCGTCAAGGAAGAAGGGGGCTTGACTTTGGGCGCAATAGAGAAGATGAGGCTGCTGAAATCCGTCGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAGTACGCGCGGGCGAAAGAAGATATCGTGATCCAAAGCCACGATTTCTCCTACAAGATAAAGAAAGGGGAGATGATTTTTGGGTATCAGCCGATCGCGACCAGAGACCCGAAGGTTTTCGAGAATCCGGAGAAGTTCGTCGGCGACAGGTTCGTCGGAGAGAAAGGGGAGAAGCTTCTGAATTATGTTTACTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGCGAAGGATCTGGTGGTGCTGGTGGCCAGGCTAATTCTGGTGGAGCTCTTCCTCCGTTACGACTCGTTTACCGTCGAGGGGACTCAGTCGTCGCTCGGATGGACAGTGAAGATCAAATCGTTGACCAAATCGACCGCTAAGGTTTGA

Coding sequence (CDS)

ATGAGTTCCTCTTCTTCTTCCTCCGAACGGCCACTGAAACCCATTCCCGGCGGCTACGGCTTCTCCATTTTTGGGCCCATCAAGGATCGCTACGACTACTTCTACATCCAAGGCCGAGAAAAGTTCTTCCGAACCCGGATGAGTAAATACGGGTCAACCGTTTTCCGGGTCAACATGCCGCCAGGTCCCTTCATCTCCTCCGATTCCAGAGTCATCGTCCTCCTCGACGCTGTCAGTTTTCCGATCCTCTTCGACACCACCAAGGTCGAGAAACGCAACGTTCTTGACGGAACTTACATGCCGTCGGTGGCCTTCACCGGCAATATTCGCACGTGCGCTTTTCTTGACCCGTCGGAGCCAGAGCACTCTGCTCTCAAGCGCCTGTTCCTCTCTTTTCTCGCCTCTCGCCACGACAAGTTTGTTCCCCTGTTCCGGAGCTCCTTGTCGGATATGTTCGATAAGCTGGACGGGAAATTGGAGGAAAAGAACGCCGCTGCCGCCGATTTCAATCCGATGAGTGACGCGGCGGCGTTTGACTTCATCTTCCGGCTGCTCACCGATCGGAGCCCCGACGAGAAATTGGGATCCGAGGGGCCGGGTCTTGTCGATAAATGGCTGGCCATGCAGCTCGCGCCGTTGGCTTCTGCCGGCCTCCCCAAATTCCTCAGCTGCTTCGAAGATCTCGTCATCCACACTTTTCCATTGCCCTTCTTCCCCGTCAAAAGTTCCTACAGGAAGCTGTACGATGCGTTTTACGCATCCTCTGTTTCGTTTCTGGACGAAGCAGAGAGACAGGGGATAGACAGAGAGGAAGCCTGCCACAATTTAGTGTTCCTGGCCGGATTCAACGCGTACGCCGGAATGAAGGTGATGCTTCCGATTCTGCTCAATTGGGTCGGGTCCGCCGGCGAAGGGCTCCACCGGGAGCTCGCCGAGGAGGTCAGAACCGTCGTCAAGGAAGAAGGGGGCTTGACTTTGGGCGCAATAGAGAAGATGAGGCTGCTGAAATCCGTCGTGTACGAGGTTCTGAGGATCGACCCGCCGGTGCCGTACCAGTACGCGCGGGCGAAAGAAGATATCGTGATCCAAAGCCACGATTTCTCCTACAAGATAAAGAAAGGGGAGATGATTTTTGGGTATCAGCCGATCGCGACCAGAGACCCGAAGGTTTTCGAGAATCCGGAGAAGTTCGTCGGCGACAGGTTCGTCGGAGAGAAAGGGGAGAAGCTTCTGAATTATGTTTACTGGTCGAACGGCCGGGAGACGGAGGAGCCGACGGCGGAGAACAAGCAGTGTCCGGCGAAGGATCTGGTGGTGCTGGTGGCCAGGCTAATTCTGGTGGAGCTCTTCCTCCGTTACGACTCGTTTACCGTCGAGGGGACTCAGTCGTCGCTCGGATGGACAGTGAAGATCAAATCGTTGACCAAATCGACCGCTAAGGTTTGA

Protein sequence

MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAKV
Homology
[Close]
BLAST of Moc04g06190 vs. NCBI nr
Match: XP_022136336.1 (allene oxide synthase 3-like [Momordica charantia])

HSP 1 Score: 961.1 bits (2483), Expect = 3.7e-276
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0 

Query: 1   MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP 60
           MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP
Sbjct: 1   MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP 60

Query: 61  PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP 120
           PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP
Sbjct: 61  PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP 120

Query: 121 EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF 180
           EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF
Sbjct: 121 EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF 180

Query: 181 IFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPV 240
           IFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPV
Sbjct: 181 IFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPV 240

Query: 241 KSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVG 300
           KSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVG
Sbjct: 241 KSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVG 300

Query: 301 SAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDI 360
           SAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDI
Sbjct: 301 SAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDI 360

Query: 361 VIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGR 420
           VIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGR
Sbjct: 361 VIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGR 420

Query: 421 ETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAK 480
           ETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAK
Sbjct: 421 ETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAK 480

Query: 481 V 482
           V
Sbjct: 481 V 481

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BLAST of Moc04g06190 vs. NCBI nr
Match: XP_038888826.1 (allene oxide synthase 3-like [Benincasa hispida])

HSP 1 Score: 786.9 bits (2031), Expect = 9.5e-224
Identity = 379/483 (78.47%), Postives = 440/483 (91.10%), Query Frame = 0 

Query: 1   MSSSSSSSE----RPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFR 60
           M+SS SS +     PLKPIPG YGF IFGPIKDRYDYFYIQGREKFFRTRM+KY STVFR
Sbjct: 1   MTSSPSSEQPLIPLPLKPIPGSYGFPIFGPIKDRYDYFYIQGREKFFRTRMAKYDSTVFR 60

Query: 61  VNMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLD 120
            NMPPGPFISS+S+VIVLLDA+SFPILFDTTKVEKRNVLDGTYMPS+AFTG IRTCAFLD
Sbjct: 61  TNMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLD 120

Query: 121 PSEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAA 180
           PSE EHS LKR FL FLASRH +F+PLFRSS+S+MF+KLD +LE+     ADFNP+SD+A
Sbjct: 121 PSETEHSLLKRHFLKFLASRHHQFIPLFRSSVSEMFNKLDRELEKNK--VADFNPVSDSA 180

Query: 181 AFDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLP 240
           +FDF+FRLL+DRSPD+ L S+GPGLVD+WLA+QLAPLA+ GLPKFLSCFED++IHTF LP
Sbjct: 181 SFDFVFRLLSDRSPDKSLSSKGPGLVDRWLALQLAPLATLGLPKFLSCFEDIIIHTFRLP 240

Query: 241 FFPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILL 300
           F   +S+Y KLY++FY SS SFLDEAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPI+L
Sbjct: 241 FALARSAYSKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIIL 300

Query: 301 NWVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARA 360
           NWVGSAGEGLHR+LA E+R  VKE+GG+T  A+EKM LLKSV+YEVLRIDPPVPYQYA+A
Sbjct: 301 NWVGSAGEGLHRKLAVEIRAAVKEDGGVTFSALEKMSLLKSVIYEVLRIDPPVPYQYAKA 360

Query: 361 KEDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYW 420
           K+DIVI+SHD ++KIKKGEMIFGYQPIAT+DP+VFENPE+F+GDRFVGEKGEKLL YVYW
Sbjct: 361 KQDIVIESHDSAFKIKKGEMIFGYQPIATKDPRVFENPEEFIGDRFVGEKGEKLLKYVYW 420

Query: 421 SNGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTK 480
           SNGRETEEPTAENKQC A+DLVVLV+R++LVELFLRYD+F VEGTQSSLGW+VK+KSL+K
Sbjct: 421 SNGRETEEPTAENKQCAARDLVVLVSRVVLVELFLRYDTFAVEGTQSSLGWSVKVKSLSK 480

[Close]
BLAST of Moc04g06190 vs. NCBI nr
Match: XP_023554237.1 (allene oxide synthase 3-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 779.2 bits (2011), Expect = 2.0e-221
Identity = 380/480 (79.17%), Postives = 430/480 (89.58%), Query Frame = 0 

Query: 3   SSSSSSERP-----LKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 62
           +SSSSSE P     LKPIPGGYG   FGPIK RYDYFY QGREKFFR+RM++YGSTVFR 
Sbjct: 2   TSSSSSEHPPAPLSLKPIPGGYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRT 61

Query: 63  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 122
           NMPPGPFISSDSRV+VLLDA SFPILFDTTKVEKRNVLDGTYMPSV+FTG IRTCAFLDP
Sbjct: 62  NMPPGPFISSDSRVVVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDP 121

Query: 123 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 182
           SE EHS LKR FL FLASRHDKF+PLFR+S+S+MF KL+ +LE+ N  AADFN +SDAA+
Sbjct: 122 SETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNN--AADFNSVSDAAS 181

Query: 183 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 242
           FDFIFRLL+D  PDEKLGS+G  LVD+WL MQLAPLA+ GLPKFLSCFEDL+IHTFPLPF
Sbjct: 182 FDFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPF 241

Query: 243 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 302
             VK++YRKLYDAFY SS +FL+EAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPI+LN
Sbjct: 242 ALVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILN 301

Query: 303 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 362
           WVGSAG+ LHR+LA E+R  VKE  GLT  AIE M LLKSVVYEVLRIDPPVPYQYA+AK
Sbjct: 302 WVGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAK 361

Query: 363 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 422
           +DI+I+SHD +++IKKGEMIFGYQPIATRDPKVFE PE+F+GDRFVGE+GEKLL YVYWS
Sbjct: 362 QDIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWS 421

Query: 423 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKS 478
           NGRETEEPT ENKQCPA+DLVVLV+R++L ELFLRYD+F VEGTQSSLGW+VK+KSLTK+
Sbjct: 422 NGRETEEPTTENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTKA 479

[Close]
BLAST of Moc04g06190 vs. NCBI nr
Match: XP_022952977.1 (allene oxide synthase 3-like [Cucurbita moschata])

HSP 1 Score: 778.9 bits (2010), Expect = 2.6e-221
Identity = 381/479 (79.54%), Postives = 429/479 (89.56%), Query Frame = 0 

Query: 3   SSSSSSER-----PLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 62
           +SSSSSE      PLKPIPG YG   FGPIK RYDYFY QGREKFFR+RM++YGSTVFR 
Sbjct: 2   TSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRT 61

Query: 63  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 122
           NMPPGPFISSDSRVIVLLDA SFPILFDTTKVEKRNVLDGTYMPSV+FTG IRTCAFLDP
Sbjct: 62  NMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDP 121

Query: 123 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 182
           SE EHS LKR FL FLASRHDKF+PLFR+S+S+MF KL+ +LE+ N  AADFN +SDAA+
Sbjct: 122 SETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNN--AADFNSVSDAAS 181

Query: 183 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 242
           FDFIFRLL+D  PDEKLGS+G  LVD+WL MQLAPLA+ GLPKFLSCFEDL+IHTFPLPF
Sbjct: 182 FDFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPF 241

Query: 243 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 302
             VK++YRKLYDAFY SS +FL+EAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPI+LN
Sbjct: 242 ALVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILN 301

Query: 303 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 362
           WVGSAG+ LH++LA E+R  VKE  GLT  AIE M LLKSVVYEVLRIDPPVPYQYA+AK
Sbjct: 302 WVGSAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAK 361

Query: 363 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 422
           +DI+I+SHD ++KIKKGEMIFGYQPIATRDPKVFE PE+F+GDRFVGE+GEKLL YVYWS
Sbjct: 362 QDIIIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWS 421

Query: 423 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTK 477
           NGRETEEPTAENKQCPA+DLVVLV+R++L ELFLRYD+F VEGTQSSLGW+VK+KSLTK
Sbjct: 422 NGRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

[Close]
BLAST of Moc04g06190 vs. NCBI nr
Match: KAG6572010.1 (Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 778.5 bits (2009), Expect = 3.4e-221
Identity = 381/480 (79.38%), Postives = 430/480 (89.58%), Query Frame = 0 

Query: 3   SSSSSSER-----PLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 62
           +SSSSSE      PLKPIPG YG   FGPIK RYDYFY QGREKFFR+RM++YGSTVFR 
Sbjct: 2   TSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRT 61

Query: 63  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 122
           NMPPGPFISSDSRVIVLLDA SFPILFDTTKVEKRNVLDGTYMPSV+FTG IRTCAFLDP
Sbjct: 62  NMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDP 121

Query: 123 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 182
           SE EHS LKR FL FLASRHDKF+PLFR+S+S+MF KL+ +LE+ N  AADFN +SDAA+
Sbjct: 122 SETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNN--AADFNSVSDAAS 181

Query: 183 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 242
           FDFIFRLL+D  PDEKLGS+G  LVD+WL MQLAPLA+ GLPKFLSCFEDL+IHTFPLPF
Sbjct: 182 FDFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPF 241

Query: 243 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 302
             VK++YRKLYDAFY SS +FL+EAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPI+LN
Sbjct: 242 AFVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILN 301

Query: 303 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 362
           WVGSAG+ LHR+LA E+R  VKE  GLT  AIE M LLKSVVYEVLRIDPPVPYQYA+AK
Sbjct: 302 WVGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAK 361

Query: 363 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 422
           +DI+I+SHD +++IKKGEMIFGYQPIATRDPKVFE PE+F+GDRFVGE+GEKLL YVYWS
Sbjct: 362 QDIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWS 421

Query: 423 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKS 478
           NGRETEEPTAENKQCPA+DLVVLV+R++L ELFLRYD+F VEGTQSSLGW+VK+KSLTK+
Sbjct: 422 NGRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTKA 479

[Close]
BLAST of Moc04g06190 vs. ExPASy Swiss-Prot
Match: Q8GZP5 (Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1)

HSP 1 Score: 544.3 bits (1401), Expect = 1.4e-153
Identity = 263/480 (54.79%), Postives = 353/480 (73.54%), Query Frame = 0 

Query: 2   SSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMPP 61
           +  SS    P+K IPG YG   FG IKDRYD+ Y QG ++FFR+RM KY STVFR N+PP
Sbjct: 15  TKESSIPSLPMKEIPGDYGVPFFGAIKDRYDFHYNQGADEFFRSRMKKYDSTVFRTNVPP 74

Query: 62  GPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEPE 121
           GPF + +S+V+VL+DAVS+PILFD ++V+K N  +GT+M S +F G  + C FL  S+P+
Sbjct: 75  GPFNARNSKVVVLVDAVSYPILFDNSQVDKENYFEGTFMSSPSFNGGYKVCGFLGTSDPK 134

Query: 122 HSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDFI 181
           H+ LK LFLS L   HDKF+P+F +S++ MF  L+ +L EK  +   FNP+ D  +F+F+
Sbjct: 135 HTTLKGLFLSTLTRLHDKFIPIFTTSITSMFTSLEKELSEKGTSY--FNPIGDNLSFEFL 194

Query: 182 FRLLTD-RSP-DEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFP 241
           FRL  + ++P D  +G  GP +VDKW+ +QLAPL S GL    +  EDLV+HTFPLP+  
Sbjct: 195 FRLFCEGKNPIDTSVGPNGPKIVDKWVFLQLAPLISLGLKFVPNFLEDLVLHTFPLPYIL 254

Query: 242 VKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWV 301
           VK  ++KLY+AFY S    LDEAE+ G+ R+EACHN VFLAGFN+Y G+KV  P L+ W+
Sbjct: 255 VKRDHQKLYNAFYNSMKDILDEAEKLGVKRDEACHNFVFLAGFNSYGGLKVFFPSLIKWI 314

Query: 302 GSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKED 361
           G++G  LH  L +E+RT VKE GG+TL AI+KM L+KSVVYE LR+DPPVP+Q  +A+++
Sbjct: 315 GTSGPSLHARLVKEIRTAVKEAGGVTLSAIDKMPLVKSVVYETLRMDPPVPFQTVKARKN 374

Query: 362 IVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNG 421
           I+I +H+ S+ IKK E+IFGYQP+AT+D KVF+N E+F  DRFVG  GEKLL YVYWSNG
Sbjct: 375 IIITNHESSFLIKKDELIFGYQPLATKDSKVFKNAEEFNPDRFVG-GGEKLLKYVYWSNG 434

Query: 422 RETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTA 480
           +E + P+  +KQCP KDL+VL+ RL++VE F+RYD+F VE  +  LG  V  KSLTK+T+
Sbjct: 435 KEIDNPSVNDKQCPGKDLIVLMGRLLVVEFFMRYDTFEVEFGKLLLGSKVTFKSLTKATS 491

[Close]
BLAST of Moc04g06190 vs. ExPASy Swiss-Prot
Match: K4BV52 (Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 PE=2 SV=1)

HSP 1 Score: 514.2 bits (1323), Expect = 1.6e-144
Identity = 256/483 (53.00%), Postives = 345/483 (71.43%), Query Frame = 0 

Query: 2   SSSSS----SSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 61
           SSSSS     ++ P + +PG YG  + GP KDR DYFY QG+ +FF++R+ K+ STVFR 
Sbjct: 56  SSSSSPPVKQAKLPAQKVPGDYGLPLVGPWKDRLDYFYNQGKNEFFKSRIQKHQSTVFRT 115

Query: 62  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 121
           NMPPGPFIS +  V+VLLD  SFP+LFD +KVEK+++  GT+MPS   TG  R  ++LDP
Sbjct: 116 NMPPGPFISFNPNVVVLLDGKSFPVLFDVSKVEKKDLFTGTFMPSTDLTGGYRVLSYLDP 175

Query: 122 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 181
           SEP H+ LK+L    L+SR ++ +P F +S S++F+ L+ +L  K  A    N  +D AA
Sbjct: 176 SEPNHAKLKKLMFYLLSSRRNEVIPEFHNSYSELFETLENELSTKGKAG--LNAANDQAA 235

Query: 182 FDFIFRLLTDRSP-DEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLP 241
           F+F+ R L   +P D +LG++GP L+ KW+  QL PL   GLPK L   EDLV+HTF LP
Sbjct: 236 FNFLARSLYGINPQDTELGTDGPKLIGKWVLFQLHPLLILGLPKVL---EDLVMHTFRLP 295

Query: 242 FFPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILL 301
              VK  Y++LY+ FY +S S LDEAE+ GI REEACHNL+F   FN++ G+K+  P +L
Sbjct: 296 PALVKKDYQRLYNFFYENSTSVLDEAEKIGISREEACHNLLFATCFNSFGGIKIFFPNML 355

Query: 302 NWVGSAGEGLHRELAEEVRTVVKEEGG-LTLGAIEKMRLLKSVVYEVLRIDPPVPYQYAR 361
            W+G AG  LH +LA+E+R+V+    G +T+ A+EKM L+KSVVYE LRI+PPV  QY R
Sbjct: 356 KWIGRAGAKLHSQLAQEIRSVISSNSGKVTMAAMEKMPLMKSVVYESLRIEPPVASQYGR 415

Query: 362 AKEDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVY 421
           AK D+VI+SHD S++IK+GE+++GYQP AT+DPK+F+  E+FV DRF+GE+GEKLL +V 
Sbjct: 416 AKHDMVIESHDASFEIKEGELLYGYQPFATKDPKIFDRSEEFVADRFIGEEGEKLLKHVL 475

Query: 422 WSNGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLT 479
           WSNG ETE  +  NKQC  KD VVLV+RL+LVELFLRYDSF +E   S LG  + + SL 
Sbjct: 476 WSNGSETENASINNKQCAGKDFVVLVSRLLLVELFLRYDSFEIEVGASPLGAAITLTSLR 533

[Close]
BLAST of Moc04g06190 vs. ExPASy Swiss-Prot
Match: Q96242 (Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A PE=1 SV=3)

HSP 1 Score: 503.4 bits (1295), Expect = 2.8e-141
Identity = 250/480 (52.08%), Postives = 336/480 (70.00%), Query Frame = 0 

Query: 1   MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP 60
           +++ + S + P++ IPG YG  I GPIKDR+DYFY QG E+FF++R+ KY STV+RVNMP
Sbjct: 43  VATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMP 102

Query: 61  PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP 120
           PG FI+ + +V+ LLD  SFP+LFD  KVEK+++  GTYMPS   TG  R  ++LDPSEP
Sbjct: 103 PGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEP 162

Query: 121 EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF 180
           +H  LK L    L S  ++  P F+++ S++FD L+ +L  K    ADF   SD  AF+F
Sbjct: 163 KHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLK--GKADFGGSSDGTAFNF 222

Query: 181 IFRLLTDRSP-DEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFP 240
           + R     +P D KL ++ PGL+ KW+   L PL S GLP+ +   E+ +IHTF LP   
Sbjct: 223 LARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVI---EEPLIHTFSLPPAL 282

Query: 241 VKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWV 300
           VKS Y++LY+ F  S+   L EA++ GI REEA HNL+F   FN + GMK++ P ++  +
Sbjct: 283 VKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRI 342

Query: 301 GSAGEGLHRELAEEVRTVVKEEGG-LTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKE 360
           G AG  +H  LAEE+R+V+K  GG LT+GAIEKM L KSVVYE LR +PPV  QY RAK+
Sbjct: 343 GRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKK 402

Query: 361 DIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSN 420
           D+VI+SHD ++K+K GEM++GYQP+ATRDPK+F+  ++FV +RFVGE+GEKLL +V WSN
Sbjct: 403 DLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSN 462

Query: 421 GRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKST 479
           G ETE PT  NKQC  KD VVLVARL ++E+F RYDSF +E   S LG +V   SL K++
Sbjct: 463 GPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

[Close]
BLAST of Moc04g06190 vs. ExPASy Swiss-Prot
Match: Q7XYS3 (Allene oxide synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP74A2 PE=2 SV=2)

HSP 1 Score: 503.1 bits (1294), Expect = 3.6e-141
Identity = 251/472 (53.18%), Postives = 327/472 (69.28%), Query Frame = 0 

Query: 11  PLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMPPGPFISSDSR 70
           P +P+PG YG      ++DR D++Y+QG++K+F +R  +YGSTV R+N+PPGPF++ D R
Sbjct: 8   PRRPVPGSYGVPFVSAVRDRLDFYYLQGQDKYFESRAERYGSTVVRINVPPGPFMARDPR 67

Query: 71  VIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEPEHSALKRLFL 130
           V+ LLDA SFP+LFD  KVEKR+V  GT+MPS + TG  R CA+LDPSEP H+ +K+L L
Sbjct: 68  VVALLDAKSFPVLFDVAKVEKRDVFTGTFMPSTSLTGGYRVCAYLDPSEPNHAKIKQLLL 127

Query: 131 SFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDFIFRLLTDRSP 190
           S L SR D FVP+FRS+   + D ++ +L       +DF  ++DA +F+FI        P
Sbjct: 128 SLLVSRKDAFVPVFRSNFGALLDTVESQL-ASGGGKSDFTALNDATSFEFIGEAYFGVRP 187

Query: 191 --DEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPVKSSYRKLY 250
                LG+ GP     WL  QLAPL + GLP  +   ED ++HT PLP F + S Y+ LY
Sbjct: 188 SASSSLGTGGPTKAALWLLWQLAPLTTLGLPMII---EDPLLHTLPLPPFLISSDYKALY 247

Query: 251 DAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVGSAGEGLHR 310
             F A++   LD AE  G+ REEACHNL+F   FN+Y G K++LP +L+ V  AGE LH 
Sbjct: 248 AYFAAAASQALDAAEGLGLSREEACHNLLFATVFNSYGGFKLLLPQILSRVAQAGEKLHE 307

Query: 311 ELAEEVRTVVKEEGG-LTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDIVIQSHDF 370
            LA E+R+ V + GG +TL A+EKM L +SVV+E LR+DPPV +QY RAK D+ I+SHD 
Sbjct: 308 RLAAEIRSAVADAGGNVTLAALEKMELTRSVVWEALRLDPPVRFQYGRAKADLEIESHDA 367

Query: 371 SYKIKKGEMIFGYQPIATRDPKVF-ENPEKFVGDRFVGEKGEKLLNYVYWSNGRETEEPT 430
           S+ IKKGEM+FGYQP ATRDP+VF     +FVGDRFVGE+G KLL YVYWSNGRETE P+
Sbjct: 368 SFAIKKGEMLFGYQPCATRDPRVFGATAREFVGDRFVGEEGRKLLQYVYWSNGRETENPS 427

Query: 431 AENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKST 479
            +NKQCP K+LVVLV RL+LVELFLRYD+FT E      G  V I  +TK++
Sbjct: 428 VDNKQCPGKNLVVLVGRLLLVELFLRYDTFTAEA-----GKKVVITGVTKAS 470

[Close]
BLAST of Moc04g06190 vs. ExPASy Swiss-Prot
Match: Q9LLB0 (Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 PE=1 SV=1)

HSP 1 Score: 501.9 bits (1291), Expect = 8.0e-141
Identity = 244/467 (52.25%), Postives = 330/467 (70.66%), Query Frame = 0 

Query: 13  KPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMPPGPFISSDSRVI 72
           + IPG YG    GP KDR DYFY QG+  FF +R++KY ST+FR NMPPGPFI+S+ +VI
Sbjct: 49  RTIPGDYGLPGIGPWKDRLDYFYNQGKNDFFESRIAKYKSTIFRTNMPPGPFITSNPKVI 108

Query: 73  VLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEPEHSALKRLFLSF 132
           VLLD  SFP+LFD +KVEK+++  GT++PS   TG  R  ++LDPSEP H  LK+L    
Sbjct: 109 VLLDGKSFPVLFDASKVEKKDLFTGTFVPSTELTGGYRILSYLDPSEPNHEKLKKLMFFL 168

Query: 133 LASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDFIFRLLTDRSPDE 192
           L+SR D  +P F  + +++F+ LD ++EEK      FN  SD AAF+F+ R L   +P E
Sbjct: 169 LSSRRDHVIPEFHETYTELFETLDKEMEEKGTVG--FNSGSDQAAFNFLARSLFGVNPVE 228

Query: 193 -KLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPVKSSYRKLYDAF 252
            KLG++GP L+ KW+ +QL P+ + GLPKFL   +D+++HTF LP   VK  Y++LYD F
Sbjct: 229 TKLGTDGPALIGKWILLQLHPVITLGLPKFL---DDVLLHTFRLPPILVKKDYQRLYDFF 288

Query: 253 YASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVGSAGEGLHRELA 312
           Y +S +   EAE+ GI ++EACHNL+F   FN++ GMK+  P +L  +  AG  +H  LA
Sbjct: 289 YTNSANLFIEAEKLGISKDEACHNLLFATCFNSFGGMKIFFPNMLKSIAKAGVEIHTRLA 348

Query: 313 EEVRTVVKEEGG-LTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDIVIQSHDFSYK 372
            E+R+ VK  GG +T+ A+EKM L+KSVVYE LR+DPPV  QY RAK+D+ I+SHD  ++
Sbjct: 349 NEIRSEVKSAGGKITMSAMEKMPLMKSVVYEALRVDPPVASQYGRAKQDLKIESHDAVFE 408

Query: 373 IKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGRETEEPTAENK 432
           +KKGE++FGYQP AT+DPK+F+ P +FV DRFVGE+GEKLL +V WSNG ETE PT  NK
Sbjct: 409 VKKGEILFGYQPFATKDPKIFDRPGEFVADRFVGEEGEKLLKHVLWSNGPETESPTVGNK 468

Query: 433 QCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKS 478
           QC  KD VV+V+RL + E FLRY +  V+   S+LG ++ I SL K+
Sbjct: 469 QCAGKDFVVMVSRLFVTEFFLRYGTLNVDVGTSALGSSITITSLKKA 510

[Close]
BLAST of Moc04g06190 vs. ExPASy TrEMBL
Match: A0A6J1C386 (allene oxide synthase 3-like OS=Momordica charantia OX=3673 GN=LOC111008047 PE=4 SV=1)

HSP 1 Score: 961.1 bits (2483), Expect = 1.8e-276
Identity = 481/481 (100.00%), Postives = 481/481 (100.00%), Query Frame = 0 

Query: 1   MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP 60
           MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP
Sbjct: 1   MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP 60

Query: 61  PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP 120
           PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP
Sbjct: 61  PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP 120

Query: 121 EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF 180
           EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF
Sbjct: 121 EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF 180

Query: 181 IFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPV 240
           IFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPV
Sbjct: 181 IFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFPV 240

Query: 241 KSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVG 300
           KSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVG
Sbjct: 241 KSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVG 300

Query: 301 SAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDI 360
           SAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDI
Sbjct: 301 SAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDI 360

Query: 361 VIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGR 420
           VIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGR
Sbjct: 361 VIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGR 420

Query: 421 ETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAK 480
           ETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAK
Sbjct: 421 ETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKSTAK 480

Query: 481 V 482
           V
Sbjct: 481 V 481

[Close]
BLAST of Moc04g06190 vs. ExPASy TrEMBL
Match: A0A6J1GLX0 (allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.3e-221
Identity = 381/479 (79.54%), Postives = 429/479 (89.56%), Query Frame = 0 

Query: 3   SSSSSSER-----PLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 62
           +SSSSSE      PLKPIPG YG   FGPIK RYDYFY QGREKFFR+RM++YGSTVFR 
Sbjct: 2   TSSSSSEHPPAPLPLKPIPGSYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRT 61

Query: 63  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 122
           NMPPGPFISSDSRVIVLLDA SFPILFDTTKVEKRNVLDGTYMPSV+FTG IRTCAFLDP
Sbjct: 62  NMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDP 121

Query: 123 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 182
           SE EHS LKR FL FLASRHDKF+PLFR+S+S+MF KL+ +LE+ N  AADFN +SDAA+
Sbjct: 122 SETEHSVLKRHFLKFLASRHDKFIPLFRNSVSEMFSKLEKELEKNN--AADFNSVSDAAS 181

Query: 183 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 242
           FDFIFRLL+D  PDEKLGS+G  LVD+WL MQLAPLA+ GLPKFLSCFEDL+IHTFPLPF
Sbjct: 182 FDFIFRLLSDGKPDEKLGSDGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPF 241

Query: 243 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 302
             VK++YRKLYDAFY SS +FL+EAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPI+LN
Sbjct: 242 ALVKNAYRKLYDAFYDSSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILN 301

Query: 303 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 362
           WVGSAG+ LH++LA E+R  VKE  GLT  AIE M LLKSVVYEVLRIDPPVPYQYA+AK
Sbjct: 302 WVGSAGKELHKKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAK 361

Query: 363 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 422
           +DI+I+SHD ++KIKKGEMIFGYQPIATRDPKVFE PE+F+GDRFVGE+GEKLL YVYWS
Sbjct: 362 QDIIIESHDSAFKIKKGEMIFGYQPIATRDPKVFEKPEEFIGDRFVGEEGEKLLKYVYWS 421

Query: 423 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTK 477
           NGRETEEPTAENKQCPA+DLVVLV+R++L ELFLRYD+F VEGTQSSLGW+VK+KSLTK
Sbjct: 422 NGRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFAVEGTQSSLGWSVKVKSLTK 478

[Close]
BLAST of Moc04g06190 vs. ExPASy TrEMBL
Match: A0A6J1IB84 (allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.1e-221
Identity = 381/480 (79.38%), Postives = 430/480 (89.58%), Query Frame = 0 

Query: 3   SSSSSSERP-----LKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 62
           +SSSSSE P     LKPIPGGYG   FGPIK RYDYFY QGREKFFR+RM++YGSTVFR 
Sbjct: 2   TSSSSSEHPPAPPSLKPIPGGYGLPFFGPIKSRYDYFYFQGREKFFRSRMAQYGSTVFRT 61

Query: 63  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 122
           NMPPGPFISSDSRVIVLLDA SFPILFDTTKVEKRNVLDGTYMPSV+FTG IRTCAFLDP
Sbjct: 62  NMPPGPFISSDSRVIVLLDARSFPILFDTTKVEKRNVLDGTYMPSVSFTGGIRTCAFLDP 121

Query: 123 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 182
           SE EHS LKR FL FLASRHDKF+PLFR+S+S+MF KL+ +LE+ N  AADFN +SDAA+
Sbjct: 122 SETEHSVLKRHFLKFLASRHDKFIPLFRTSVSEMFSKLEKELEKTN--AADFNSVSDAAS 181

Query: 183 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 242
           FDFIFRLL+D  P EKLGS G  LVD+WL MQLAPLA+ GLPKFLSCFEDL+IHTFPLPF
Sbjct: 182 FDFIFRLLSDGKPGEKLGSNGHSLVDRWLIMQLAPLATLGLPKFLSCFEDLIIHTFPLPF 241

Query: 243 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 302
             VK++YRKLYDAFY SS +FL+EAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPI+LN
Sbjct: 242 ALVKNAYRKLYDAFYESSETFLNEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPIILN 301

Query: 303 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 362
           WVGSAG+ LHR+LA E+R  VKE  GLT  AIE M LLKSVVYEVLRIDPPVPYQYA+AK
Sbjct: 302 WVGSAGKELHRKLAGEIRAAVKENRGLTFTAIENMSLLKSVVYEVLRIDPPVPYQYAKAK 361

Query: 363 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 422
           +DI+I+SHD +++IKKGEMIFGYQPIATRDPKVFE PE+F+G+RFVGE+GEKLL YVYWS
Sbjct: 362 QDIIIESHDSAFEIKKGEMIFGYQPIATRDPKVFEKPEEFIGERFVGEEGEKLLKYVYWS 421

Query: 423 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKS 478
           NGRETEEPTAENKQCPA+DLVVLV+R++L ELFLRYD+FTVEGTQSSLGW+VK+KSLTK+
Sbjct: 422 NGRETEEPTAENKQCPARDLVVLVSRVVLAELFLRYDTFTVEGTQSSLGWSVKVKSLTKA 479

[Close]
BLAST of Moc04g06190 vs. ExPASy TrEMBL
Match: A0A0A0K2U8 (Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4 SV=1)

HSP 1 Score: 770.8 bits (1989), Expect = 3.4e-219
Identity = 375/480 (78.12%), Postives = 430/480 (89.58%), Query Frame = 0 

Query: 1   MSSSSSSSER---PLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 60
           M+SSSS   +   PLKPIPG YGF IFGPI DRY YFYIQGRE FFR+RM+KY STVFR 
Sbjct: 1   MTSSSSEHPQIPLPLKPIPGSYGFPIFGPIIDRYHYFYIQGRETFFRSRMAKYNSTVFRT 60

Query: 61  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 120
           NMPPGPFISS+S+VIVLLDA++FPILFDTTKVEKRNVLDGTYMPS+AFTG IRTCAFLDP
Sbjct: 61  NMPPGPFISSNSKVIVLLDALTFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 120

Query: 121 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 180
           SE EHS LKR FL FLASRH +F+PLFR+S+S+MFDKL+ +L+  N   A+FNP+SD A+
Sbjct: 121 SETEHSVLKRHFLKFLASRHHQFIPLFRTSISEMFDKLEKELQNNN--VANFNPISDYAS 180

Query: 181 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 240
           FDFIFRLL+DRSPD+   SEGPGLVD+WL MQLAPLA+ GLPK  SCFEDL+IHTF LPF
Sbjct: 181 FDFIFRLLSDRSPDKNFSSEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPF 240

Query: 241 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 300
             VKS+YRKLY++FY SS SFLDEAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+LPILLN
Sbjct: 241 ALVKSAYRKLYESFYESSGSFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLLPILLN 300

Query: 301 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 360
           WVGSAGE LHR+L  E+R  VK +GG+T GA+EKM LLKSVVYEVLRIDPPVPYQYA+AK
Sbjct: 301 WVGSAGEELHRKLVGEIRAAVKIDGGITFGALEKMSLLKSVVYEVLRIDPPVPYQYAKAK 360

Query: 361 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 420
           +DIVI+SHD +++IKKGEMIFGYQPIAT+DPKVFEN E+FVG+RFVGEKGEKLL YVYWS
Sbjct: 361 QDIVIESHDSAFEIKKGEMIFGYQPIATKDPKVFENAEEFVGERFVGEKGEKLLKYVYWS 420

Query: 421 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKS 478
           NGRETEEPTAENKQCPA+DLVVL+ R++LVELFLRYD+FTVEGT+SSLGW+VK+KSLTK+
Sbjct: 421 NGRETEEPTAENKQCPARDLVVLMCRVVLVELFLRYDTFTVEGTRSSLGWSVKVKSLTKA 478

[Close]
BLAST of Moc04g06190 vs. ExPASy TrEMBL
Match: A0A5A7SKU9 (Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G00100 PE=4 SV=1)

HSP 1 Score: 765.4 bits (1975), Expect = 1.4e-217
Identity = 374/480 (77.92%), Postives = 430/480 (89.58%), Query Frame = 0 

Query: 3   SSSSSSER-----PLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRV 62
           +SSSS E      PLKPIPG  GF IFGPIKDRY YFYIQGRE FFR+RM+KY STVFR 
Sbjct: 2   TSSSSPEHPHIPLPLKPIPGTNGFPIFGPIKDRYHYFYIQGRETFFRSRMAKYNSTVFRT 61

Query: 63  NMPPGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDP 122
           NMPPGPFISS+S+VIVLLDA+SFPILFDTTKVEKRNVLDGTYMPS+AFTG IRTCAFLDP
Sbjct: 62  NMPPGPFISSNSKVIVLLDALSFPILFDTTKVEKRNVLDGTYMPSLAFTGGIRTCAFLDP 121

Query: 123 SEPEHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAA 182
           SE EHS LKR FL+FLASRH +F+PLFR+S+S+MFDKL+ +L  KN   A+FNP+SD+A+
Sbjct: 122 SETEHSVLKRHFLNFLASRHHQFIPLFRTSISEMFDKLEKEL--KNNNVANFNPVSDSAS 181

Query: 183 FDFIFRLLTDRSPDEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPF 242
           FDFIFRLL+DRSP++   SEGPGLVD+WL MQLAPLA+ GLPK  SCFEDL+IHTF LPF
Sbjct: 182 FDFIFRLLSDRSPNKNFISEGPGLVDRWLTMQLAPLATLGLPKIFSCFEDLIIHTFRLPF 241

Query: 243 FPVKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLN 302
             VKS+YRKLY++FY SS +FLDEAE+QGI+RE+ACHNLVFLAGFNAYAGMKV+ PILLN
Sbjct: 242 ALVKSAYRKLYESFYESSGTFLDEAEKQGINREKACHNLVFLAGFNAYAGMKVLFPILLN 301

Query: 303 WVGSAGEGLHRELAEEVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAK 362
           WVGSAGE LHR+L  E+R  VK++ G+T GA+EKM LLKSVVYEVLRIDPPVPYQYARAK
Sbjct: 302 WVGSAGEELHRKLVSEIRAAVKKDEGVTFGALEKMSLLKSVVYEVLRIDPPVPYQYARAK 361

Query: 363 EDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWS 422
           +DIVI++HD ++KIKKGEMIFGYQPIAT+DPKVFEN E+FVGDRFVGEKGEKLL YVYWS
Sbjct: 362 QDIVIENHDSAFKIKKGEMIFGYQPIATKDPKVFENAEEFVGDRFVGEKGEKLLKYVYWS 421

Query: 423 NGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKS 478
           NGRETEEPTAENKQCPA+DLVVL++R++LVELFLRYD+F VEGT+SSLGW+VK+KSLTK+
Sbjct: 422 NGRETEEPTAENKQCPARDLVVLMSRVVLVELFLRYDTFAVEGTRSSLGWSVKVKSLTKA 479

[Close]
BLAST of Moc04g06190 vs. TAIR 10
Match: AT5G42650.1 (allene oxide synthase )

HSP 1 Score: 503.4 bits (1295), Expect = 2.0e-142
Identity = 250/480 (52.08%), Postives = 336/480 (70.00%), Query Frame = 0 

Query: 1   MSSSSSSSERPLKPIPGGYGFSIFGPIKDRYDYFYIQGREKFFRTRMSKYGSTVFRVNMP 60
           +++ + S + P++ IPG YG  I GPIKDR+DYFY QG E+FF++R+ KY STV+RVNMP
Sbjct: 43  VATRTGSKDLPIRNIPGNYGLPIVGPIKDRWDYFYDQGAEEFFKSRIRKYNSTVYRVNMP 102

Query: 61  PGPFISSDSRVIVLLDAVSFPILFDTTKVEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEP 120
           PG FI+ + +V+ LLD  SFP+LFD  KVEK+++  GTYMPS   TG  R  ++LDPSEP
Sbjct: 103 PGAFIAENPQVVALLDGKSFPVLFDVDKVEKKDLFTGTYMPSTELTGGYRILSYLDPSEP 162

Query: 121 EHSALKRLFLSFLASRHDKFVPLFRSSLSDMFDKLDGKLEEKNAAAADFNPMSDAAAFDF 180
           +H  LK L    L S  ++  P F+++ S++FD L+ +L  K    ADF   SD  AF+F
Sbjct: 163 KHEKLKNLLFFLLKSSRNRIFPEFQATYSELFDSLEKELSLK--GKADFGGSSDGTAFNF 222

Query: 181 IFRLLTDRSP-DEKLGSEGPGLVDKWLAMQLAPLASAGLPKFLSCFEDLVIHTFPLPFFP 240
           + R     +P D KL ++ PGL+ KW+   L PL S GLP+ +   E+ +IHTF LP   
Sbjct: 223 LARAFYGTNPADTKLKADAPGLITKWVLFNLHPLLSIGLPRVI---EEPLIHTFSLPPAL 282

Query: 241 VKSSYRKLYDAFYASSVSFLDEAERQGIDREEACHNLVFLAGFNAYAGMKVMLPILLNWV 300
           VKS Y++LY+ F  S+   L EA++ GI REEA HNL+F   FN + GMK++ P ++  +
Sbjct: 283 VKSDYQRLYEFFLESAGEILVEADKLGISREEATHNLLFATCFNTWGGMKILFPNMVKRI 342

Query: 301 GSAGEGLHRELAEEVRTVVKEEGG-LTLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKE 360
           G AG  +H  LAEE+R+V+K  GG LT+GAIEKM L KSVVYE LR +PPV  QY RAK+
Sbjct: 343 GRAGHQVHNRLAEEIRSVIKSNGGELTMGAIEKMELTKSVVYECLRFEPPVTAQYGRAKK 402

Query: 361 DIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSN 420
           D+VI+SHD ++K+K GEM++GYQP+ATRDPK+F+  ++FV +RFVGE+GEKLL +V WSN
Sbjct: 403 DLVIESHDAAFKVKAGEMLYGYQPLATRDPKIFDRADEFVPERFVGEEGEKLLRHVLWSN 462

Query: 421 GRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSLGWTVKIKSLTKST 479
           G ETE PT  NKQC  KD VVLVARL ++E+F RYDSF +E   S LG +V   SL K++
Sbjct: 463 GPETETPTVGNKQCAGKDFVVLVARLFVIEIFRRYDSFDIEVGTSPLGSSVNFSSLRKAS 517

[Close]
BLAST of Moc04g06190 vs. TAIR 10
Match: AT4G15440.1 (hydroperoxide lyase 1 )

HSP 1 Score: 284.3 bits (726), Expect = 1.9e-76
Identity = 154/388 (39.69%), Postives = 229/388 (59.02%), Query Frame = 0 

Query: 89  VEKRNVLDGTYMPSVAFTGNIRTCAFLDPSEPEHSALKRLFLSFLASRHDKFVPLFRSSL 148
           V+KR+VL G + PS+ F G +R   +LD +EP+H+ +K   +  L      ++   RS+L
Sbjct: 4   VDKRDVLIGDFRPSLGFYGGVRVGVYLDTTEPKHAKIKGFAMETLKRSSKVWLQELRSNL 63

Query: 149 SDMFDKLDGKLEEKNAAAADFNPMSDAAAFDFIFRLL--TDRSPDEKLGSEGPGLVDKWL 208
           +  +  ++ ++  KN AA+   P+     F F+   L   D S    +   G   ++ WL
Sbjct: 64  NIFWGTIESEI-SKNGAASYIFPL-QRCIFSFLCASLAGVDASVSPDIAENGWKTINTWL 123

Query: 209 AMQLAPLASAG-LPKFLSCFEDLVIHTFPLPFFPVKSSYRKLYDAFYASSVSFLDEAERQ 268
           A+Q+ P A  G +P+ L   E++++HT+P P   +  +Y+KLY+    ++   L   + +
Sbjct: 124 ALQVIPTAKLGVVPQPL---EEILLHTWPYPSLLIAGNYKKLYNFIDENAGDCLRLGQEE 183

Query: 269 -GIDREEACHNLVFLAGFNAYAGMKVMLPILLNWVGSAGEGLHRELAEEVRTVVKEEGGL 328
            G+ R+EA  NL+F+ GFNAY G  V LP L+  +     GL   +  EVR V      L
Sbjct: 184 FGLTRDEAIQNLLFVLGFNAYGGFSVFLPSLIGRITGDNSGLQERIRTEVRRVCGSGSDL 243

Query: 329 TLGAIEKMRLLKSVVYEVLRIDPPVPYQYARAKEDIVIQSHDFSYKIKKGEMIFGYQPIA 388
               + +M L+KSVVYE LR  PPVP Q+ARA++D  I SHD  +++KKGE++ GYQP+ 
Sbjct: 244 NFKTVNEMELVKSVVYETLRFSPPVPLQFARARKDFQISSHDAVFEVKKGELLCGYQPLV 303

Query: 389 TRDPKVFENPEKFVGDRFVGEKGEKLLNYVYWSNGRETEEPTAENKQCPAKDLVVLVARL 448
            RD  VF+ PE+F  DR+VGE G +LLNY+YWSNG +T  P+A NKQC AKD+V L A L
Sbjct: 304 MRDANVFDEPEEFKPDRYVGETGSELLNYLYWSNGPQTGTPSASNKQCAAKDIVTLTASL 363

Query: 449 ILVELFLRYDSFTVEGTQSSLGWTVKIK 473
           ++ +LFLRYD  T+ G   S+   VK K
Sbjct: 364 LVADLFLRYD--TITGDSGSIKAVVKAK 384

[Close]
BLAST of Moc04g06190 vs. TAIR 10
Match: AT5G25180.1 (cytochrome P450, family 71, subfamily B, polypeptide 14 )

HSP 1 Score: 57.8 bits (138), Expect = 2.8e-08
Identity = 47/150 (31.33%), Postives = 73/150 (48.67%), Query Frame = 0 

Query: 313 EVRTVVKEEGGLTLGAIEKMRLLKSVVYEVLRIDPPVPYQYAR-AKEDIVIQSHDFSYKI 372
           EVR V+K +  +T   IE++  LK V+ E LRI+P VP    R A + I I  +D    I
Sbjct: 326 EVREVIKNKDDITEEDIERLEYLKMVIKETLRINPLVPLLIPREASKYIKIGGYD----I 385

Query: 373 KKGEMIFGYQPIATRDPKVFENPEKFVGDRFV-GEKGEKLLNYVYWSNGRETEEPTAENK 432
            K   I+       R+P V+++PE F+ +RF+  E   K +++     G       +  +
Sbjct: 386 PKKTWIYVNIWAVQRNPNVWKDPEVFIPERFMHSEIDYKGVDFELLPFG-------SGRR 445

Query: 433 QCPAKDLVVLVARLILVELFLRYDSFTVEG 461
            CP   L + +  L L+ L  R+D    EG
Sbjct: 446 MCPGMGLGMALVHLTLINLLYRFDWKLPEG 464

[Close]
BLAST of Moc04g06190 vs. TAIR 10
Match: AT5G25140.1 (cytochrome P450, family 71, subfamily B, polypeptide 13 )

HSP 1 Score: 57.4 bits (137), Expect = 3.7e-08
Identity = 54/188 (28.72%), Postives = 86/188 (45.74%), Query Frame = 0 

Query: 290 VMLPILLNWVGSAGEGL-------------HRELAEEVRTVVKEEGGLTLGAIEKMRLLK 349
           ++L ILL  V ++G  +              ++   EVR V+K +  +T   IE +  LK
Sbjct: 290 ILLDILLAGVDTSGHTITWVMTHLIKNPRVMKKAQAEVREVIKNKDNITEEDIEGLEYLK 349

Query: 350 SVVYEVLRIDPPVPYQYAR-AKEDIVIQSHDFSYKIKKGEMIFGYQPIATRDPKVFENPE 409
            VV E LRI+P VP    R A +D+ I      Y I K   I        R+P V+++PE
Sbjct: 350 MVVKETLRINPLVPLLTPREASKDVKIG----GYNIPKKTWIHVNIWAIHRNPNVWKDPE 409

Query: 410 KFVGDRFVGEKGE-KLLNYVYWSNGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYD 463
            F+ +RF+  + + K LN+     G       +  + CP   + + +  L L+ L  R+D
Sbjct: 410 AFIPERFMDNQIDYKGLNFELLPFG-------SGRRICPGIGMGMALIHLTLINLLYRFD 466

[Close]
BLAST of Moc04g06190 vs. TAIR 10
Match: AT1G64930.1 (cytochrome P450, family 87, subfamily A, polypeptide 7 )

HSP 1 Score: 52.4 bits (124), Expect = 1.2e-06
Identity = 58/195 (29.74%), Postives = 88/195 (45.13%), Query Frame = 0 

Query: 295 LLNWVGS---AGEGLHRELAEEVRTVVKEEGGLTLGA-IEKMRLLKSVVYEVLRIDPP-- 354
           +L W+ +     + +   L EE+  VV EE  +      +KM  LK+VV E LR  PP  
Sbjct: 319 VLQWIMANLVKNQEIQERLYEEITNVVGEEAKVVEEKDTQKMPYLKAVVMEALRRHPPGN 378

Query: 355 --VPYQYARAKEDIVIQSHDFSYKI-KKGEMIFGYQPIATRDPKVFENPEKFVGDRFVGE 414
             +P+      ED V+      YK+ KKG + F    I  RDPKV+E P  F  +RF+GE
Sbjct: 379 TVLPHS---VTEDTVLG----GYKVPKKGTINFLVAEIG-RDPKVWEEPMAFKPERFMGE 438

Query: 415 KGEKLLNYVYWSNGRETEEPTAENKQCPAKDLVVLVARLILVELFLRYDSFTVEGTQSSL 474
             E+ ++ +  S G +     A  + CP   L +L     +  +   +    VEG +  L
Sbjct: 439 --EEAVD-ITGSRGIKMMPFGAGRRICPGIGLAMLHLEYYVANMVREFQWKEVEGHEVDL 498

Query: 475 GWTVKIKSLTKSTAK 481
              V+   + K   K
Sbjct: 499 TEKVEFTVIMKHPLK 502

The following BLAST results are available for this feature:
BLAST of Moc04g06190 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022136336.13.7e-276100.00allene oxide synthase 3-like [Momordica charantia][more]
XP_038888826.19.5e-22478.47allene oxide synthase 3-like [Benincasa hispida][more]
XP_023554237.12.0e-22179.17allene oxide synthase 3-like [Cucurbita pepo subsp. pepo][more]
XP_022952977.12.6e-22179.54allene oxide synthase 3-like [Cucurbita moschata][more]
KAG6572010.13.4e-22179.38Allene oxide synthase 3, partial [Cucurbita argyrosperma subsp. sororia][more]
BLAST of Moc04g06190 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8GZP51.4e-15354.79Allene oxide synthase 3 OS=Solanum lycopersicum OX=4081 GN=AOS3 PE=1 SV=1[more]
K4BV521.6e-14453.00Allene oxide synthase 1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS1 P... [more]
Q962422.8e-14152.08Allene oxide synthase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CYP74A P... [more]
Q7XYS33.6e-14153.18Allene oxide synthase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP74A2 PE=2... [more]
Q9LLB08.0e-14152.25Allene oxide synthase 2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=AOS2 P... [more]
BLAST of Moc04g06190 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1C3861.8e-276100.00allene oxide synthase 3-like OS=Momordica charantia OX=3673 GN=LOC111008047 PE=4... [more]
A0A6J1GLX01.3e-22179.54allene oxide synthase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111455491 PE=4 ... [more]
A0A6J1IB842.1e-22179.38allene oxide synthase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111470925 PE=4 SV... [more]
A0A0A0K2U83.4e-21978.13Fatty acid 9-hydroperoxide lyase OS=Cucumis sativus OX=3659 GN=Csa_7G075600 PE=4... [more]
A0A5A7SKU91.4e-21777.92Allene oxide synthase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sca... [more]
BLAST of Moc04g06190 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G42650.12.0e-14252.08allene oxide synthase [more]
AT4G15440.11.9e-7639.69hydroperoxide lyase 1 [more]
AT5G25180.12.8e-0831.33cytochrome P450, family 71, subfamily B, polypeptide 14 [more]
AT5G25140.13.7e-0828.72cytochrome P450, family 71, subfamily B, polypeptide 13 [more]
AT1G64930.11.2e-0629.74cytochrome P450, family 87, subfamily A, polypeptide 7 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002403Cytochrome P450, E-class, group IVPRINTSPR00465EP450IVcoord: 332..348
score: 37.1
coord: 385..403
score: 31.38
coord: 369..383
score: 20.0
IPR001128Cytochrome P450PFAMPF00067p450coord: 308..415
e-value: 1.8E-18
score: 66.6
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 6..479
e-value: 5.2E-112
score: 377.1
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 10..464
NoneNo IPR availablePANTHERPTHR24286:SF302HYDROPEROXIDE LYASEcoord: 11..458
NoneNo IPR availablePANTHERPTHR24286CYTOCHROME P450 26coord: 11..458

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g06190.1Moc04g06190.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016125 sterol metabolic process
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen