Moc04g05200 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc04g05200 vs. NCBI nr
Match: XP_022136050.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Momordica charantia])

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 690/690 (100.00%), Postives = 690/690 (100.00%), Query Frame = 0

Query: 1   MEDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAK 60
           MEDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAK
Sbjct: 1   MEDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAK 60

Query: 61  NDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHD 120
           NDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHD
Sbjct: 61  NDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHD 120

Query: 121 IEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNK 180
           IEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNK
Sbjct: 121 IEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNK 180

Query: 181 DVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVND 240
           DVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVND
Sbjct: 181 DVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVND 240

Query: 241 DSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFG 300
           DSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFG
Sbjct: 241 DSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFG 300

Query: 301 ESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLG 360
           ESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLG
Sbjct: 301 ESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLG 360

Query: 361 ADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLD 420
           ADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLD
Sbjct: 361 ADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLD 420

Query: 421 FIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRID 480
           FIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRID
Sbjct: 421 FIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRID 480

Query: 481 SLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWH 540
           SLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWH
Sbjct: 481 SLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWH 540

Query: 541 RHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDT 600
           RHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDT
Sbjct: 541 RHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDT 600

Query: 601 KGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIK 660
           KGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIK
Sbjct: 601 KGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIK 660

Query: 661 ISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           ISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 ISEMLLGKVPDGFSFAPKHKSNAYDRAENG 690

BLAST of Moc04g05200 vs. NCBI nr
Match: KAA0049681.1 (tRNA-dihydrouridine(47) synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 615/692 (88.87%), Postives = 643/692 (92.92%), Query Frame = 0

Query: 2   EDSVPA---EPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPST 61
           EDS+ A    P   SA NG   TP++LVAKAIAPV P +LRPPP+RSSS N  KDA  ST
Sbjct: 3   EDSLTAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSS-NTLKDAT-ST 62

Query: 62  AKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFS 121
           A ND  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRYSDKCRFS
Sbjct: 63  ANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFS 122

Query: 122 HDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGL 181
           HDIEAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGL
Sbjct: 123 HDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGL 182

Query: 182 NKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKV 241
           NKD+QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKEDKE+ VSNDSVV DRTCCS+ 
Sbjct: 183 NKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSET 242

Query: 242 NDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKA 301
           N+D D DLEI VEV EENG KEGTFSSDESRPLKK KS++S NF SDQIDNGGACM ++ 
Sbjct: 243 NNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGGACMIDET 302

Query: 302 FGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 361
            GE    IESEVMND V+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV
Sbjct: 303 IGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 362

Query: 362 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 421
           LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV
Sbjct: 363 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 422

Query: 422 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNR 481
           LDFIDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNR
Sbjct: 423 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNR 482

Query: 482 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLD 541
           IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY D
Sbjct: 483 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 542

Query: 542 WHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 601
           W++HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS
Sbjct: 543 WNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 602

Query: 602 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADW 661
           D+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADW
Sbjct: 603 DSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 662

Query: 662 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 692

BLAST of Moc04g05200 vs. NCBI nr
Match: XP_038888663.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1238.0 bits (3202), Expect = 0.0e+00
Identity = 621/700 (88.71%), Postives = 645/700 (92.14%), Query Frame = 0

Query: 1   MEDSVPA----------EPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNN 60
           MEDSV A          EP   SA NG QLTP++LVAKAIAPVK  +LRPPPVRSSS NN
Sbjct: 1   MEDSVTAELLDDTAVSEEPVSHSADNGTQLTPQQLVAKAIAPVKREFLRPPPVRSSSSNN 60

Query: 61  AKDAAPSTAKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCR 120
            KDA  STAK+D  DK  PS LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCR
Sbjct: 61  VKDAT-STAKSDSDDKPAPSGLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCR 120

Query: 121 YSDKCRFSHDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLK 180
           YSDKCRFSHDIEAFKSQKPADL+GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK
Sbjct: 121 YSDKCRFSHDIEAFKSQKPADLDGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLK 180

Query: 181 KSSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVP 240
           K SEMNGLNKDVQKLLWKNKMKFPKADSKLKELGL   GKSKLRVKEDKE+ VSND VV 
Sbjct: 181 KCSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLLAQGKSKLRVKEDKENAVSNDIVVA 240

Query: 241 DRTCCSKVNDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNG 300
           DRTCCSKVN+D D DLEI V+V EEN  +EGTFSSDESRPLKK KS++SENF SDQ+DN 
Sbjct: 241 DRTCCSKVNNDLDADLEISVKVPEENEQEEGTFSSDESRPLKKSKSLSSENFHSDQVDN- 300

Query: 301 GACMAEKAFGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNL 360
           GA M ++  GE  T IESEVMNDSV+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNL
Sbjct: 301 GARMIDETIGEGHTPIESEVMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNL 360

Query: 361 PFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAV 420
           PFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAV
Sbjct: 361 PFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAV 420

Query: 421 ELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRT 480
           ELI+KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVE ASGIVDTPITIKVRT
Sbjct: 421 ELINKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEAASGIVDTPITIKVRT 480

Query: 481 GYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGN 540
           GYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAP+SLQVIGN
Sbjct: 481 GYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPESLQVIGN 540

Query: 541 GDVFSYLDWHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVH 600
           GDVFSY DW+RH+DE PKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVH
Sbjct: 541 GDVFSYSDWNRHRDEGPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVH 600

Query: 601 FGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLM 660
           FGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLM
Sbjct: 601 FGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLM 660

Query: 661 ASDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           +SDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 SSDSAADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of Moc04g05200 vs. NCBI nr
Match: XP_004148548.1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like [Cucumis sativus] >KGN43192.1 hypothetical protein Csa_020245 [Cucumis sativus])

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 611/689 (88.68%), Postives = 642/689 (93.18%), Query Frame = 0

Query: 2   EDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAKN 61
           + +V   P   S+ NG  LTP++LVAKAIAPVK  +LRPPP+RSSS NN KDA  STAKN
Sbjct: 13  DTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSS-NNLKDAT-STAKN 72

Query: 62  DDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDI 121
           D  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDI
Sbjct: 73  DGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDI 132

Query: 122 EAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNKD 181
           EAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGLNKD
Sbjct: 133 EAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKD 192

Query: 182 VQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVNDD 241
           +QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKED+E+ VSNDSVV DR CCS++N+D
Sbjct: 193 IQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRPCCSEMNND 252

Query: 242 SDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFGE 301
            D DLEI VEV EENG KEGTFSSDESRPLKK KS++SENF SDQIDN GACM ++  GE
Sbjct: 253 LDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-GACMIDETIGE 312

Query: 302 SDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGA 361
               IESE MNDSV+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGA
Sbjct: 313 GHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGA 372

Query: 362 DVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDF 421
           DVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDF
Sbjct: 373 DVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDF 432

Query: 422 IDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRIDS 481
           IDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDS
Sbjct: 433 IDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDS 492

Query: 482 LIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWHR 541
           LIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY DW+ 
Sbjct: 493 LIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNE 552

Query: 542 HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTK 601
           HK+ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLEHWGSD+K
Sbjct: 553 HKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSK 612

Query: 602 GVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIKI 661
           GVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADWIKI
Sbjct: 613 GVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKI 672

Query: 662 SEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           SEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 673 SEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of Moc04g05200 vs. NCBI nr
Match: XP_008448025.1 (PREDICTED: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like isoform X1 [Cucumis melo] >TYK12191.1 tRNA-dihydrouridine(47) synthase [Cucumis melo var. makuwa])

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 614/692 (88.73%), Postives = 642/692 (92.77%), Query Frame = 0

Query: 2   EDSVPA---EPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPST 61
           EDS+ A    P   SA NG   TP++LVAKAIAPV P +LRPPP+RSSS N  KDA  ST
Sbjct: 3   EDSLTAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSS-NTLKDAT-ST 62

Query: 62  AKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFS 121
           A ND  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRYSDKCRFS
Sbjct: 63  ANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFS 122

Query: 122 HDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGL 181
           HDIEAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGL
Sbjct: 123 HDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGL 182

Query: 182 NKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKV 241
           NKD+QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKEDKE+ VSNDSVV DRTCCS+ 
Sbjct: 183 NKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSET 242

Query: 242 NDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKA 301
           N+D D DLEI VEV EENG KEGTFSSDESRPLKK KS++S NF SDQIDN GACM ++ 
Sbjct: 243 NNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDN-GACMIDET 302

Query: 302 FGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 361
            GE    IESEVMND V+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV
Sbjct: 303 IGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 362

Query: 362 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 421
           LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV
Sbjct: 363 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 422

Query: 422 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNR 481
           LDFIDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNR
Sbjct: 423 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNR 482

Query: 482 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLD 541
           IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY D
Sbjct: 483 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 542

Query: 542 WHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 601
           W++HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS
Sbjct: 543 WNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 602

Query: 602 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADW 661
           D+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADW
Sbjct: 603 DSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 662

Query: 662 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of Moc04g05200 vs. ExPASy Swiss-Prot
Match: Q7XT07 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0117600 PE=2 SV=2)

HSP 1 Score: 877.5 bits (2266), Expect = 1.0e-253
Identity = 441/694 (63.54%), Postives = 528/694 (76.08%), Query Frame = 0

Query: 6   PAEPTPQS--ASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAKNDD 65
           PA+P   S  AS+  + +PEELVA+A+APVKP +LRPP               +T   D+
Sbjct: 13  PADPPDSSPAASSPPRPSPEELVARAVAPVKPAFLRPP-------------LSATPPKDE 72

Query: 66  ADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIEA 125
                  ++V EKKSKRQLKRER+QEQKS+ HLC E+ K+G+VSSC+Y D CRFSHDI+A
Sbjct: 73  GKANGGGAVVAEKKSKRQLKRERKQEQKSSSHLCIEVGKSGNVSSCKYGDSCRFSHDIDA 132

Query: 126 FKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNKDVQ 185
           + +QKPADL G CPF++ +QLCPYGL CRF GTHK+ + A S  NL +  E+N LNKD+Q
Sbjct: 133 YLAQKPADLEGTCPFTNLDQLCPYGLTCRFLGTHKD-IHAASG-NLSEKHEINALNKDIQ 192

Query: 186 KLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKED-----KEDVVSNDSVVPDRTCCSKV 245
           KLLWKNK KFPKA +++K LGL    KSK     D      +++  ND    +  C   V
Sbjct: 193 KLLWKNKYKFPKASAQIKLLGLKEVIKSKPDAANDDKKVNHDNLDGNDDENKEPLCNPPV 252

Query: 246 NDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKA 305
           N + D  L   ++ SE   L + +    E RP KK K        SD+ID  GA      
Sbjct: 253 NAECDSTLCEELDRSEGEPLIDNSIPCVEPRPTKKSK------VESDEIDKHGAGTLNTN 312

Query: 306 FGESDTQIES--EVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVC 365
               D  + +  E  N+S    TD    PH REKK+IDFR+KLYLAPLTTVGNLPFRR+C
Sbjct: 313 TESEDPNLSNGLEPSNNSSSCRTDLITTPHLREKKIIDFREKLYLAPLTTVGNLPFRRLC 372

Query: 366 KVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKE 425
           K LGAD+TCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV+R VEL+D E
Sbjct: 373 KTLGADITCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVARTVELVDNE 432

Query: 426 CVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGK 485
           C +DFIDINMGCPIDIVVNKGAGS+LLTKPMR+KS+V+ AS + + P+T+KVRT +FEG+
Sbjct: 433 CSVDFIDINMGCPIDIVVNKGAGSSLLTKPMRIKSIVQAASTVTEKPLTVKVRTAFFEGR 492

Query: 486 NRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSY 545
           NR DS+++DI +WGASA+T+HGR+RQQRYSKLADW+YI++C+  APD L V+GNGDVFS+
Sbjct: 493 NRADSIVSDIYDWGASAITVHGRSRQQRYSKLADWDYIYQCAQKAPDQLHVVGNGDVFSF 552

Query: 546 LDWHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHW 605
            DW++H   C K++T MIARGALIKPW+FTE+KEQR+WDITSGER NILKDFV FGLEHW
Sbjct: 553 TDWNKHVSGCSKISTSMIARGALIKPWIFTEVKEQRHWDITSGERFNILKDFVSFGLEHW 612

Query: 606 GSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAA 665
           GSD+KGVETTR+FLLEWLSYTCRYIPVGLLDV+PQRLNWRPPSY GRDDLETLM SDSAA
Sbjct: 613 GSDSKGVETTRYFLLEWLSYTCRYIPVGLLDVIPQRLNWRPPSYCGRDDLETLMISDSAA 672

Query: 666 DWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           DWI+ISEMLLGKVP+GF+F PKHKSNAYDRAENG
Sbjct: 673 DWIRISEMLLGKVPEGFTFTPKHKSNAYDRAENG 685

BLAST of Moc04g05200 vs. ExPASy Swiss-Prot
Match: Q9T0J6 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Arabidopsis thaliana OX=3702 GN=At4g38890 PE=1 SV=2)

HSP 1 Score: 874.8 bits (2259), Expect = 6.5e-253
Identity = 449/696 (64.51%), Postives = 547/696 (78.59%), Query Frame = 0

Query: 4   SVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAKNDD 63
           ++P + T Q +   A  T EEL+ +++AP+K  +L PPP            + S  +ND 
Sbjct: 21  ALPVQSTKQFSVYEA--TSEELIERSMAPIKKEFLCPPP-----------PSRSVKQNDA 80

Query: 64  AD-KAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIE 123
           AD +AP S LV+EKKSKRQLKRERR++  ST +LCP++A+T DV SC+Y DKCRF+HDIE
Sbjct: 81  ADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDVDSCQYKDKCRFNHDIE 140

Query: 124 AFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNKDV 183
           AFK+QK  D+ G+CPF ++   C YGL+CRF G+H++    G+S + K+ SEMN  NK+ 
Sbjct: 141 AFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNSDD-KEKSEMNFFNKET 200

Query: 184 QKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVNDDS 243
           Q+LLWKNKM F  AD+KLK LGL GH K     +E   +   N     + T  ++V  DS
Sbjct: 201 QRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNGM---NGTQATEVAVDS 260

Query: 244 DDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDN--GGACMAEK--- 303
               E   E+ ++  +  G   ++E RP+KK KS   +N ++  +     G  + E+   
Sbjct: 261 AVSSEHTSEMIQDVDI-PGPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKK 320

Query: 304 -AFGESDTQIESEVMNDSV-ILDTDASLKPHPRE-KKLIDFRDKLYLAPLTTVGNLPFRR 363
             +  S   +E E   DS+ I++TD+SLK HPRE KKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 321 NGYPTSKANVEDE---DSIKIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRR 380

Query: 364 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 423
           +CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICG+YPDTVSR VELID
Sbjct: 381 LCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGSYPDTVSRVVELID 440

Query: 424 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFE 483
           +EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VEV+S IV+TPITIKVRT +FE
Sbjct: 441 RECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFE 500

Query: 484 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVF 543
           GKNRIDSLIADIGNWGA+AVTIHGR+RQQRYSK ADW+YI++C+  A  +LQVIGNGDV+
Sbjct: 501 GKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVY 560

Query: 544 SYLDWHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 603
           SYLDW++HK +CP+L++CMIARGALIKPW+FTEIKEQR+WDITSGERLNI+KDFV FGL+
Sbjct: 561 SYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQ 620

Query: 604 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDS 663
           HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++NWRPPSYFGRDDLETLM S+S
Sbjct: 621 HWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQINWRPPSYFGRDDLETLMMSES 680

Query: 664 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           A DW++ISEMLLGKVP+GF+FAPKHKSNAYDRAENG
Sbjct: 681 AGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691

BLAST of Moc04g05200 vs. ExPASy Swiss-Prot
Match: Q7ZWS1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus laevis OX=8355 GN=dus3l PE=2 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 5.3e-146
Identity = 288/636 (45.28%), Postives = 384/636 (60.38%), Query Frame = 0

Query: 49  NNAKDAAPSTAKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSS 108
           +  +D  P TA+N    K        + KS+  +K  + +E K    LCP + +    S 
Sbjct: 89  SEGQDQPPKTAENKQEKKRARG----QNKSRPHMKHSQFEENK----LCPSVTQE-CASK 148

Query: 109 CRYSDKCRFSHDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSIN 168
           C + DKC+FSHD+  + SQKP D+   C        C YG+ CRFA +H  G    + IN
Sbjct: 149 CFFGDKCKFSHDVAKYVSQKPEDIRPNCHLYETFGKCIYGVTCRFAKSHL-GEDFKNIIN 208

Query: 169 LKKSSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSV 228
            +   E  G      K+L KN +     D  LKE         +LR K+           
Sbjct: 209 EELMKEWEG------KVLVKNSL-----DKSLKE---------QLRKKK----------- 268

Query: 229 VPDRTCCSKVNDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQID 288
                    V + SD  L++              F S +S  +K P              
Sbjct: 269 --------VVFEKSDKYLKL-------------CFKSGDSSKMKNP-------------- 328

Query: 289 NGGACMAEKAFGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVG 348
                + ++      TQ +S +     + D D  +K  P EKK IDFR+KLYLAPLTT G
Sbjct: 329 -----VVKEDSAVQVTQKDSPITTVGAVTDEDV-IKLRPCEKKTIDFRNKLYLAPLTTCG 388

Query: 349 NLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSR 408
           NLPFRR+CK LGAD+TCGEMAMCTNLLQGQ SEWALL+RH SED+FGVQ+ GA+PDT+++
Sbjct: 389 NLPFRRICKRLGADITCGEMAMCTNLLQGQPSEWALLKRHHSEDIFGVQLEGAFPDTMTK 448

Query: 409 AVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKV 468
             EL+++   +DF+DIN+GCPID+V  KG G  L+ +  + + +V+  + ++D P+T+K+
Sbjct: 449 CAELLNRTIDVDFVDINVGCPIDLVYKKGGGCGLMNRTNKFEQIVKGMNSVLDVPLTVKI 508

Query: 469 RTGYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVI 528
           RTG  E  N    LI ++ +WG S VT+HGR+R+QRY+KLA+WEYI +C+ +A   + + 
Sbjct: 509 RTGVQEKVNIAHKLIPNLRDWGVSLVTLHGRSREQRYTKLANWEYIDQCAKIA-SPVPLF 568

Query: 529 GNGDVFSYLDWHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF 588
           GNGD+ SY D +R       +A  M+ARGAL KPWLFTEIKEQR+WDI+S ER +ILKDF
Sbjct: 569 GNGDIISYEDANRALQ--TGVAGVMLARGALFKPWLFTEIKEQRHWDISSTERFDILKDF 628

Query: 589 VHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLET 648
            ++GLEHWGSD +GVE TR F+LEWLS+ CRYIPVGLL+ VPQ++N RPP Y GRD LET
Sbjct: 629 TNYGLEHWGSDCQGVEKTRKFMLEWLSFLCRYIPVGLLEHVPQKINERPPYYMGRDYLET 639

Query: 649 LMASDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 685
           LMAS +  DWIKISEMLLG VP  F+F PKHK+N+Y
Sbjct: 689 LMASQNVTDWIKISEMLLGPVPPNFTFLPKHKANSY 639

BLAST of Moc04g05200 vs. ExPASy Swiss-Prot
Match: Q28BT8 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Xenopus tropicalis OX=8364 GN=dus3l PE=2 SV=2)

HSP 1 Score: 513.5 bits (1321), Expect = 3.8e-144
Identity = 304/721 (42.16%), Postives = 407/721 (56.45%), Query Frame = 0

Query: 7   AEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPP-------------VRSSSCN---- 66
           AE    +  NG   T  + + + +A +KP YL                 V   +C+    
Sbjct: 2   AESDGSNNENGNLDTVTQKLDRGVAAIKPQYLTTKEKFHVFIDADGKEVVDKQTCSELSG 61

Query: 67  -------NAKDAAPSTAKN---DDA-----DKAPPSSLVKEKKSKRQLKRERRQEQKSTR 126
                   A+DAA   AK    DD      DK P S+  K++K + + + + R   K ++
Sbjct: 62  NDAENTVRAEDAAEPEAKRIKLDDGTGEGQDKPPTSAENKQEKKRARGQNKSRPHMKHSQ 121

Query: 127 ----HLCPEIAKTGDVSSCRYSDKCRFSHDIEAFKSQKPADLNGECPFSSAEQLCPYGLA 186
                LCP + +    S C + DKC+F HD+  + S+KP D+   C        C YG+ 
Sbjct: 122 FEENKLCPSVTQE-CASKCFFGDKCKFLHDVAKYVSEKPEDIRPNCYLYETFGKCIYGVT 181

Query: 187 CRFAGTHKEGVPAGSSIN--LKKSSE-----MNGLNKDVQKLLWKNKMKFPKADSKLKEL 246
           CRFA +H  G    + IN  L K  E      N L+K +++ L K K+ F K D  LK  
Sbjct: 182 CRFAKSHL-GDNFKNLINEELMKQWEGQVLVKNSLDKSLKEQLRKRKVVFEKTDKYLK-- 241

Query: 247 GLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVNDDSDDDLEIVVEVSEENGLKEGTF 306
                                          C+K    S D L+I   V +E        
Sbjct: 242 ------------------------------LCNK----SGDSLKIKSPVVKE-------- 301

Query: 307 SSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFGESDTQIESEVMNDSVILDTDASL 366
                                   DN    +          Q +S V     + D D  +
Sbjct: 302 ------------------------DNAAQVV----------QKDSPVTTVGAVTDEDL-V 361

Query: 367 KPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWA 426
           K  P EKK IDFR+KLYLAPLTT GNLPFRR+CK  GAD+TCGEMAMCTNLLQGQ SEWA
Sbjct: 362 KLRPCEKKTIDFRNKLYLAPLTTCGNLPFRRLCKRFGADITCGEMAMCTNLLQGQPSEWA 421

Query: 427 LLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALL 486
           LL+RH SED+FGVQ+ GA+PDT+++  EL+++   +DF+DIN+GCPID+V  KG G  L+
Sbjct: 422 LLKRHHSEDIFGVQLEGAFPDTMTKCAELLNRTIDVDFVDINVGCPIDLVYKKGGGCGLM 481

Query: 487 TKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQ 546
            +  + + +V+  + ++D P+T+K+RTG  E  N    LI ++ +WG S VT+HGR+R+Q
Sbjct: 482 NRTNKFEQIVKGMNSVLDVPLTVKIRTGVQEKINIAHKLIPNLRDWGVSLVTLHGRSREQ 541

Query: 547 RYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWHRHKDECPKLATCMIARGALIKPW 606
           RY+KLADWEYI +C+ +A   L + GNGD+ SY D +R       ++  M+ARGAL+KPW
Sbjct: 542 RYTKLADWEYIAQCADIA-SPLPLFGNGDIISYEDANRALQ--TGVSGIMLARGALLKPW 601

Query: 607 LFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPV 666
           LFTEIKEQR+WDI+S ER +ILKDF ++GLEHWGSD +GVE TR F+LEWLS+ CRYIP+
Sbjct: 602 LFTEIKEQRHWDISSTERFDILKDFTNYGLEHWGSDCQGVEKTRRFMLEWLSFLCRYIPI 638

Query: 667 GLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIKISEMLLGKVPDGFSFAPKHKSNA 685
           GLL+ VPQ++N RPP Y GRD +ETLMAS +  DWIKISEMLLG VP  FSF PKHK+N+
Sbjct: 662 GLLEHVPQKINERPPYYMGRDYMETLMASQNVTDWIKISEMLLGPVPPNFSFLPKHKANS 638

BLAST of Moc04g05200 vs. ExPASy Swiss-Prot
Match: Q96G46 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS3L PE=1 SV=2)

HSP 1 Score: 513.1 bits (1320), Expect = 5.0e-144
Identity = 278/638 (43.57%), Postives = 372/638 (58.31%), Query Frame = 0

Query: 58  TAKNDDADKAPPSSLVKEKKSKRQLKRER---RQEQKSTRHLCPEIAKTGDVSSCRYSDK 117
           TA     + A P   ++ +K  R   + R   +        LCP + +    + C + D+
Sbjct: 82  TADGQTEEAAEPGEQLQTQKRARGQNKGRPHVKPTNYDKNRLCPSLIQE-SAAKCFFGDR 141

Query: 118 CRFSHDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSI------- 177
           CRF HD+  +   KPADL   C        CPYG+ CRFAG H    P G ++       
Sbjct: 142 CRFLHDVGRYLETKPADLGPRCVLFETFGRCPYGVTCRFAGAHLR--PEGQNLVQEELAA 201

Query: 178 -NLKKSSEMNGLNKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSND 237
              +  S  NGL+K +Q+ L K +++F +A+  L+                      +  
Sbjct: 202 RGTQPPSIRNGLDKALQQQLRKREVRFERAEQALRRFSQG----------------PTPA 261

Query: 238 SVVPDRTCCSKVNDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQ 297
           + VP                             EGT +    R          EN  + Q
Sbjct: 262 AAVP-----------------------------EGTAAEGAPR---------QENCGAQQ 321

Query: 298 IDNGGACMAEKAFGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTT 357
           +  G           + T   S V     + D D  ++  P EKK +D R KLYLAPLTT
Sbjct: 322 VPAGPG---------TSTPPSSPVRTCGPLTDEDV-VRLRPCEKKRLDIRGKLYLAPLTT 381

Query: 358 VGNLPFRRVCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTV 417
            GNLPFRR+CK  GADVTCGEMA+CTNLLQGQ SEWALL+RH  ED+FGVQ+ GA+PDT+
Sbjct: 382 CGNLPFRRICKRFGADVTCGEMAVCTNLLQGQMSEWALLKRHQCEDIFGVQLEGAFPDTM 441

Query: 418 SRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITI 477
           ++  EL+ +   +DF+DIN+GCPID+V  KG G AL+ +  + + +V   + ++D P+T+
Sbjct: 442 TKCAELLSRTVEVDFVDINVGCPIDLVYKKGGGCALMNRSTKFQQIVRGMNQVLDVPLTV 501

Query: 478 KVRTGYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQ 537
           K+RTG  E  N    L+ ++ +WG + VT+HGR+R+QRY+KLADW+YI  C   A   + 
Sbjct: 502 KIRTGVQERVNLAHRLLPELRDWGVALVTLHGRSREQRYTKLADWQYIEEC-VQAASPMP 561

Query: 538 VIGNGDVFSYLDWHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILK 597
           + GNGD+ S+ D +R       +   MIARGAL+KPWLFTEIKEQR+WDI+S ERL+IL+
Sbjct: 562 LFGNGDILSFEDANRAMQ--TGVTGIMIARGALLKPWLFTEIKEQRHWDISSSERLDILR 621

Query: 598 DFVHFGLEHWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDL 657
           DF ++GLEHWGSDT+GVE TR FLLEWLS+ CRY+PVGLL+ +PQR+N RPP Y GRD L
Sbjct: 622 DFTNYGLEHWGSDTQGVEKTRRFLLEWLSFLCRYVPVGLLERLPQRINERPPYYLGRDYL 649

Query: 658 ETLMASDSAADWIKISEMLLGKVPDGFSFAPKHKSNAY 685
           ETLMAS  AADWI+ISEMLLG VP  F+F PKHK+NAY
Sbjct: 682 ETLMASQKAADWIRISEMLLGPVPPSFAFLPKHKANAY 649

BLAST of Moc04g05200 vs. ExPASy TrEMBL
Match: A0A6J1C2S3 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Momordica charantia OX=3673 GN=LOC111007837 PE=3 SV=1)

HSP 1 Score: 1399.4 bits (3621), Expect = 0.0e+00
Identity = 690/690 (100.00%), Postives = 690/690 (100.00%), Query Frame = 0

Query: 1   MEDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAK 60
           MEDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAK
Sbjct: 1   MEDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAK 60

Query: 61  NDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHD 120
           NDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHD
Sbjct: 61  NDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHD 120

Query: 121 IEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNK 180
           IEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNK
Sbjct: 121 IEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNK 180

Query: 181 DVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVND 240
           DVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVND
Sbjct: 181 DVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVND 240

Query: 241 DSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFG 300
           DSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFG
Sbjct: 241 DSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFG 300

Query: 301 ESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLG 360
           ESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLG
Sbjct: 301 ESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLG 360

Query: 361 ADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLD 420
           ADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLD
Sbjct: 361 ADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLD 420

Query: 421 FIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRID 480
           FIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRID
Sbjct: 421 FIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRID 480

Query: 481 SLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWH 540
           SLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWH
Sbjct: 481 SLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWH 540

Query: 541 RHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDT 600
           RHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDT
Sbjct: 541 RHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDT 600

Query: 601 KGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIK 660
           KGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIK
Sbjct: 601 KGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIK 660

Query: 661 ISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           ISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 661 ISEMLLGKVPDGFSFAPKHKSNAYDRAENG 690

BLAST of Moc04g05200 vs. ExPASy TrEMBL
Match: A0A5A7U7Y0 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G00190 PE=3 SV=1)

HSP 1 Score: 1241.1 bits (3210), Expect = 0.0e+00
Identity = 615/692 (88.87%), Postives = 643/692 (92.92%), Query Frame = 0

Query: 2   EDSVPA---EPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPST 61
           EDS+ A    P   SA NG   TP++LVAKAIAPV P +LRPPP+RSSS N  KDA  ST
Sbjct: 3   EDSLTAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSS-NTLKDAT-ST 62

Query: 62  AKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFS 121
           A ND  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRYSDKCRFS
Sbjct: 63  ANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFS 122

Query: 122 HDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGL 181
           HDIEAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGL
Sbjct: 123 HDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGL 182

Query: 182 NKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKV 241
           NKD+QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKEDKE+ VSNDSVV DRTCCS+ 
Sbjct: 183 NKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSET 242

Query: 242 NDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKA 301
           N+D D DLEI VEV EENG KEGTFSSDESRPLKK KS++S NF SDQIDNGGACM ++ 
Sbjct: 243 NNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDNGGACMIDET 302

Query: 302 FGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 361
            GE    IESEVMND V+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV
Sbjct: 303 IGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 362

Query: 362 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 421
           LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV
Sbjct: 363 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 422

Query: 422 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNR 481
           LDFIDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNR
Sbjct: 423 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNR 482

Query: 482 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLD 541
           IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY D
Sbjct: 483 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 542

Query: 542 WHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 601
           W++HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS
Sbjct: 543 WNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 602

Query: 602 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADW 661
           D+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADW
Sbjct: 603 DSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 662

Query: 662 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 692

BLAST of Moc04g05200 vs. ExPASy TrEMBL
Match: A0A0A0K4I1 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis sativus OX=3659 GN=Csa_7G007900 PE=3 SV=1)

HSP 1 Score: 1234.6 bits (3193), Expect = 0.0e+00
Identity = 611/689 (88.68%), Postives = 642/689 (93.18%), Query Frame = 0

Query: 2   EDSVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAKN 61
           + +V   P   S+ NG  LTP++LVAKAIAPVK  +LRPPP+RSSS NN KDA  STAKN
Sbjct: 13  DTAVSEHPLSHSSDNGTHLTPQQLVAKAIAPVKRDFLRPPPLRSSS-NNLKDAT-STAKN 72

Query: 62  DDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDI 121
           D  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDI
Sbjct: 73  DGDDKAAPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDI 132

Query: 122 EAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNKD 181
           EAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGLNKD
Sbjct: 133 EAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGLNKD 192

Query: 182 VQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVNDD 241
           +QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKED+E+ VSNDSVV DR CCS++N+D
Sbjct: 193 IQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDEENAVSNDSVVADRPCCSEMNND 252

Query: 242 SDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKAFGE 301
            D DLEI VEV EENG KEGTFSSDESRPLKK KS++SENF SDQIDN GACM ++  GE
Sbjct: 253 LDADLEISVEVPEENGEKEGTFSSDESRPLKKSKSLSSENFHSDQIDN-GACMIDETIGE 312

Query: 302 SDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGA 361
               IESE MNDSV+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGA
Sbjct: 313 GHAPIESEAMNDSVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKVLGA 372

Query: 362 DVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDF 421
           DVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDF
Sbjct: 373 DVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECVLDF 432

Query: 422 IDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRIDS 481
           IDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNRIDS
Sbjct: 433 IDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNRIDS 492

Query: 482 LIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWHR 541
           LIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY DW+ 
Sbjct: 493 LIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYTDWNE 552

Query: 542 HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTK 601
           HK+ECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDF HFGLEHWGSD+K
Sbjct: 553 HKNECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFAHFGLEHWGSDSK 612

Query: 602 GVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADWIKI 661
           GVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADWIKI
Sbjct: 613 GVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADWIKI 672

Query: 662 SEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           SEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 673 SEMLLGKVPDGFSFAPKHKSNAYDRAENG 698

BLAST of Moc04g05200 vs. ExPASy TrEMBL
Match: A0A5D3CK47 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G001380 PE=3 SV=1)

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 614/692 (88.73%), Postives = 642/692 (92.77%), Query Frame = 0

Query: 2   EDSVPA---EPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPST 61
           EDS+ A    P   SA NG   TP++LVAKAIAPV P +LRPPP+RSSS N  KDA  ST
Sbjct: 3   EDSLTAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSS-NTLKDAT-ST 62

Query: 62  AKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFS 121
           A ND  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRYSDKCRFS
Sbjct: 63  ANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFS 122

Query: 122 HDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGL 181
           HDIEAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGL
Sbjct: 123 HDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGL 182

Query: 182 NKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKV 241
           NKD+QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKEDKE+ VSNDSVV DRTCCS+ 
Sbjct: 183 NKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSET 242

Query: 242 NDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKA 301
           N+D D DLEI VEV EENG KEGTFSSDESRPLKK KS++S NF SDQIDN GACM ++ 
Sbjct: 243 NNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDN-GACMIDET 302

Query: 302 FGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 361
            GE    IESEVMND V+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV
Sbjct: 303 IGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 362

Query: 362 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 421
           LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV
Sbjct: 363 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 422

Query: 422 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNR 481
           LDFIDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNR
Sbjct: 423 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNR 482

Query: 482 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLD 541
           IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY D
Sbjct: 483 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 542

Query: 542 WHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 601
           W++HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS
Sbjct: 543 WNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 602

Query: 602 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADW 661
           D+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADW
Sbjct: 603 DSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 662

Query: 662 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of Moc04g05200 vs. ExPASy TrEMBL
Match: A0A1S3BIS3 (tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Cucumis melo OX=3656 GN=LOC103490332 PE=3 SV=1)

HSP 1 Score: 1234.2 bits (3192), Expect = 0.0e+00
Identity = 614/692 (88.73%), Postives = 642/692 (92.77%), Query Frame = 0

Query: 2   EDSVPA---EPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPST 61
           EDS+ A    P   SA NG   TP++LVAKAIAPV P +LRPPP+RSSS N  KDA  ST
Sbjct: 3   EDSLTAVSEHPLSHSADNGTHFTPQQLVAKAIAPVNPDFLRPPPLRSSS-NTLKDAT-ST 62

Query: 62  AKNDDADKAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFS 121
           A ND  DKA PS+LVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDV+SCRYSDKCRFS
Sbjct: 63  ANNDGDDKAGPSTLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVNSCRYSDKCRFS 122

Query: 122 HDIEAFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGL 181
           HDIEAFKSQKPADL GECPFS AEQLCPYGLACRFAGTHKEGVPAGSS+ LK+ SEMNGL
Sbjct: 123 HDIEAFKSQKPADLEGECPFSGAEQLCPYGLACRFAGTHKEGVPAGSSVTLKRCSEMNGL 182

Query: 182 NKDVQKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKV 241
           NKD+QKLLWKNKMKFPKADSKLKELGL GHGKSKLRVKEDKE+ VSNDSVV DRTCCS+ 
Sbjct: 183 NKDIQKLLWKNKMKFPKADSKLKELGLLGHGKSKLRVKEDKENAVSNDSVVADRTCCSET 242

Query: 242 NDDSDDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDNGGACMAEKA 301
           N+D D DLEI VEV EENG KEGTFSSDESRPLKK KS++S NF SDQIDN GACM ++ 
Sbjct: 243 NNDLDADLEIPVEVPEENGEKEGTFSSDESRPLKKSKSLSSGNFHSDQIDN-GACMIDET 302

Query: 302 FGESDTQIESEVMNDSVILDTDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 361
            GE    IESEVMND V+LD+DASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV
Sbjct: 303 IGEGHAPIESEVMNDIVLLDSDASLKPHPREKKLIDFRDKLYLAPLTTVGNLPFRRVCKV 362

Query: 362 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 421
           LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV
Sbjct: 363 LGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELIDKECV 422

Query: 422 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNR 481
           LDFIDINMGCPIDIVVNKGAGSALLTKPMRMK+VVE ASGIVDTPITIKVRTGY+EGKNR
Sbjct: 423 LDFIDINMGCPIDIVVNKGAGSALLTKPMRMKTVVEAASGIVDTPITIKVRTGYYEGKNR 482

Query: 482 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLD 541
           IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYI+RCS+LAPDSLQVIGNGDVFSY D
Sbjct: 483 IDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYINRCSSLAPDSLQVIGNGDVFSYAD 542

Query: 542 WHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 601
           W++HKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS
Sbjct: 543 WNQHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGS 602

Query: 602 DTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDSAADW 661
           D+KGVETTRHFLLEWLSYTCRYIPVGLL+VVPQRLNWRPPSYFGRDDLETLM+SDSAADW
Sbjct: 603 DSKGVETTRHFLLEWLSYTCRYIPVGLLEVVPQRLNWRPPSYFGRDDLETLMSSDSAADW 662

Query: 662 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG
Sbjct: 663 IKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691

BLAST of Moc04g05200 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 874.8 bits (2259), Expect = 4.6e-254
Identity = 449/696 (64.51%), Postives = 547/696 (78.59%), Query Frame = 0

Query: 4   SVPAEPTPQSASNGAQLTPEELVAKAIAPVKPHYLRPPPVRSSSCNNAKDAAPSTAKNDD 63
           ++P + T Q +   A  T EEL+ +++AP+K  +L PPP            + S  +ND 
Sbjct: 21  ALPVQSTKQFSVYEA--TSEELIERSMAPIKKEFLCPPP-----------PSRSVKQNDA 80

Query: 64  AD-KAPPSSLVKEKKSKRQLKRERRQEQKSTRHLCPEIAKTGDVSSCRYSDKCRFSHDIE 123
           AD +AP S LV+EKKSKRQLKRERR++  ST +LCP++A+T DV SC+Y DKCRF+HDIE
Sbjct: 81  ADVRAPQSGLVQEKKSKRQLKRERREQ--STINLCPQVARTEDVDSCQYKDKCRFNHDIE 140

Query: 124 AFKSQKPADLNGECPFSSAEQLCPYGLACRFAGTHKEGVPAGSSINLKKSSEMNGLNKDV 183
           AFK+QK  D+ G+CPF ++   C YGL+CRF G+H++    G+S + K+ SEMN  NK+ 
Sbjct: 141 AFKAQKADDIEGQCPFVASGMKCAYGLSCRFLGSHRD--ITGNSDD-KEKSEMNFFNKET 200

Query: 184 QKLLWKNKMKFPKADSKLKELGLSGHGKSKLRVKEDKEDVVSNDSVVPDRTCCSKVNDDS 243
           Q+LLWKNKM F  AD+KLK LGL GH K     +E   +   N     + T  ++V  DS
Sbjct: 201 QRLLWKNKMTFTNADAKLKSLGLLGHAKKSNAAEEITAEKTQNGM---NGTQATEVAVDS 260

Query: 244 DDDLEIVVEVSEENGLKEGTFSSDESRPLKKPKSMTSENFRSDQIDN--GGACMAEK--- 303
               E   E+ ++  +  G   ++E RP+KK KS   +N ++  +     G  + E+   
Sbjct: 261 AVSSEHTSEMIQDVDI-PGPLETEEVRPMKKAKSEDQKNSKTGDVGGVYDGVKLEEETKK 320

Query: 304 -AFGESDTQIESEVMNDSV-ILDTDASLKPHPRE-KKLIDFRDKLYLAPLTTVGNLPFRR 363
             +  S   +E E   DS+ I++TD+SLK HPRE KKLIDFRDKLYLAPLTTVGNLPFRR
Sbjct: 321 NGYPTSKANVEDE---DSIKIVETDSSLKLHPREKKKLIDFRDKLYLAPLTTVGNLPFRR 380

Query: 364 VCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGAYPDTVSRAVELID 423
           +CKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICG+YPDTVSR VELID
Sbjct: 381 LCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFGVQICGSYPDTVSRVVELID 440

Query: 424 KECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFE 483
           +EC +DFIDINMGCPID+VVNK AGSALL KP+RMK++VEV+S IV+TPITIKVRT +FE
Sbjct: 441 RECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEVSSSIVETPITIKVRTAFFE 500

Query: 484 GKNRIDSLIADIGNWGASAVTIHGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVF 543
           GKNRIDSLIADIGNWGA+AVTIHGR+RQQRYSK ADW+YI++C+  A  +LQVIGNGDV+
Sbjct: 501 GKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKSADWDYIYQCTKNATTNLQVIGNGDVY 560

Query: 544 SYLDWHRHKDECPKLATCMIARGALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLE 603
           SYLDW++HK +CP+L++CMIARGALIKPW+FTEIKEQR+WDITSGERLNI+KDFV FGL+
Sbjct: 561 SYLDWNKHKSDCPELSSCMIARGALIKPWIFTEIKEQRHWDITSGERLNIMKDFVRFGLQ 620

Query: 604 HWGSDTKGVETTRHFLLEWLSYTCRYIPVGLLDVVPQRLNWRPPSYFGRDDLETLMASDS 663
           HWGSDTKGVETTRHFLLEWLSYT RYIPVGLLDV+PQ++NWRPPSYFGRDDLETLM S+S
Sbjct: 621 HWGSDTKGVETTRHFLLEWLSYTFRYIPVGLLDVIPQQINWRPPSYFGRDDLETLMMSES 680

Query: 664 AADWIKISEMLLGKVPDGFSFAPKHKSNAYDRAENG 691
           A DW++ISEMLLGKVP+GF+FAPKHKSNAYDRAENG
Sbjct: 681 AGDWVRISEMLLGKVPEGFTFAPKHKSNAYDRAENG 691

BLAST of Moc04g05200 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 109.8 bits (273), Expect = 9.0e-24
Identity = 84/309 (27.18%), Postives = 143/309 (46.28%), Query Frame = 0

Query: 333 IDFRDKLYLAPLTTVGNLPFRRVCKVLGADVTCGE------MAMCTNLLQGQASEWALLR 392
           +D+++KL LAP+  VG L FR +    GAD+T GE      +  C   L   +     + 
Sbjct: 1   MDYQNKLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVE 60

Query: 393 RHSSEDLFGV----------QICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNK 452
           + +   +F            Q+  +      +A E++  +     IDINMGCP    +  
Sbjct: 61  KGTDNVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVAT--IDINMGCPKAFSIQG 120

Query: 453 GAGSALLTKPMRMKSVVEVASGIVDTPITIKVRTGYFEGKNRIDSLIADIGNWGASAVTI 512
           G G+ALL+KP  +  ++      +D P+T K+R    +       L   I   G  A+ +
Sbjct: 121 GMGAALLSKPELIHDILATLKRNLDVPVTCKIR--LLKSPADTVELARRIEKLGVPALAV 180

Query: 513 HGRTRQQRYSKLADWEYIHRCSTLAPDSLQVIGNGDVFSYLDWHRHKDECPKLATCMIAR 572
           HGR    R    A W+ I     +A  S+ VI NGDV  Y D+ R K      A+ M+AR
Sbjct: 181 HGRKIADRPRDPAKWDEI--ADVVAALSIPVIANGDVLEYDDFSRIK-TATGAASVMVAR 240

Query: 573 GALIKPWLFTEIKEQRNWDITSGERLNILKDFVHFGLEHWGSDTKGVETTRHFLLEWLS- 625
           GA+    +F+  K + +W+       ++ K ++   +  W +D   V++T++ + E ++ 
Sbjct: 241 GAMWNASIFSP-KGKSHWE-------DVKKKYLRKSI-LWNND---VKSTKYTIKEMIAH 290

BLAST of Moc04g05200 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 79.7 bits (195), Expect = 1.0e-14
Identity = 59/204 (28.92%), Postives = 95/204 (46.57%), Query Frame = 0

Query: 336 RDKLYLAPLTTVGNLPFRRVCKVLGADVTCGEM---AMCTNLLQGQASEWALLRRHSSED 395
           R K  +AP+     LPFR +C+  GA      M    + T   + +  E+   +      
Sbjct: 87  RPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETEKYRNQEFTTCK--EDRP 146

Query: 396 LFGVQICGAYPDTVSRAVELIDKECVLDFIDINMGCPIDIVVNKGAGSALLTKPMRMKSV 455
           LF VQ C   PDT+  A + ++  C  D++DIN+GCP  I      G+ L+     +KS+
Sbjct: 147 LF-VQFCANDPDTLLEAAKRVEPYC--DYVDINLGCPQRIARRGNYGAFLMDNLPLVKSL 206

Query: 456 VEVASGIVDTPITIKVRT-GYFEGKNRIDSLIADIGNWGASAVTIHGRTRQQRYSK--LA 515
           VE  +  ++ P++ K+R     E   +   ++ D    G S + +HGRTR ++  K   A
Sbjct: 207 VEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDA---GCSLLAVHGRTRDEKDGKKFRA 266

Query: 516 DWEYIHRCSTLAPDSLQVIGNGDV 534
           DW  I          + V+ NG+V
Sbjct: 267 DWSAIKEVKNAM--RIPVLANGNV 280