Moc04g04950 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g04950
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptioneukaryotic translation initiation factor 4B3-like
Locationchr4: 3256293 .. 3257636 (-)
RNA-Seq ExpressionMoc04g04950
SyntenyMoc04g04950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCAATGGTATCTCCATGGGGAAAGCCAGGCGCGTGGGCCCTCGACTCGGAGGAACACGAAGCTCAACTTCTGAAAGAAGAGCAGGAGCAGCAGAAACAAGCGGAGCTCGGCCTGGATTTCCCCTCCCTCAACGACGCCTTGGCCACCAAGCAGAAGAAGAAGGGCCAAACTCTCTCGTTGGCGGAGTTCAACGCCTATGGCGGCAGCGGCACCAGATCTTCCGCTCACACCGCTGCGCCCCAGGGCCTCACGCATGAAGATCTCATGGTGCTTCCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACCGCCTCGGCGGTGGCTTTAGGAATTACGGTACGAATGGTTCGTATGAGAGGAATAATAGGTATTCGATTGGGGATGATTCGTCTAATTCGAAGTGGGGTTCGTCTAGGGTTTCTGATGAGAATCGGAGGAACAATGGCGGGGGTAATAGGGGGGATTCGGGTAGGGAAATTGCACCTTCTCGGGCTGATGAGGTCGATAATTGGGCCTTAACTAAGAAACCGATGGCTGCAAATGGATTTGAGAGGAGGGATAGAGGAGGAGGGTTTTCGGATTTGCCGTCTTCGAAAGCCGACGAATCAGAGAGTTGGATTTCCAATAAGAGCAATGTTCCATCGGATGTGCGGAGATTTGGGACTAACGGCGGATTTGAAAGAGAGCGACGAGGAGGTTTTCCGACGGGCGGCGGAGGCGGTGCAGATTTAGAAAATTGGGGGAGGAAGAAAGAGGAAGGTAATAATGGTGGCAGCGTTGCGGGTAGGCCGAAGCTTAATTTGCAGCCACGGACACTGCCAGTGAGCGATGGGAAGTCGTCAGAGATATCAGGGACGGGGCTGGGGGCGAGGTCGAAGAGTTCAAATCCATTCGGAGAGGCGAGGCCGAGGGAACAGGTTCTGGCTGAGAAAGGACATGACTGGAAAAAACTCGACGAGCAACTGGAGTCGAAGATCAAAGACGAGCAACCAGAGAAGGTAGAAAACTCTAACAGCACTTCCTTTGGAAAGAAGAGCTTTGGCCGGGCATCTGAGGACCGAACAGAGAGGAGCTGGAGGAAGCCAGAGTCGACATCGGAGTCACTGGAATCTCGTCCCGGCAGGTTTGTTCCAGCATCCAATTTCTAGCTTTATAACATAAGTTTCTTCCCTTTTGTTGCCATAATCACATCAGCTAACTTCACAAAAGTATCTTGACATTGTTCTATATTCTGATATGATCTCACCTGATTGTTGCTTTACAGTGCGGAGGAGAATGAAAATGGAGATGTTGAAGGGGAGTGTGAAGGGGACGAAAATGGAGCTTGA

mRNA sequence

ATGGCGGCAATGGTATCTCCATGGGGAAAGCCAGGCGCGTGGGCCCTCGACTCGGAGGAACACGAAGCTCAACTTCTGAAAGAAGAGCAGGAGCAGCAGAAACAAGCGGAGCTCGGCCTGGATTTCCCCTCCCTCAACGACGCCTTGGCCACCAAGCAGAAGAAGAAGGGCCAAACTCTCTCGTTGGCGGAGTTCAACGCCTATGGCGGCAGCGGCACCAGATCTTCCGCTCACACCGCTGCGCCCCAGGGCCTCACGCATGAAGATCTCATGGTGCTTCCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACCGCCTCGGCGGTGGCTTTAGGAATTACGGTACGAATGGTTCGTATGAGAGGAATAATAGGTATTCGATTGGGGATGATTCGTCTAATTCGAAGTGGGGTTCGTCTAGGGTTTCTGATGAGAATCGGAGGAACAATGGCGGGGGTAATAGGGGGGATTCGGGTAGGGAAATTGCACCTTCTCGGGCTGATGAGGTCGATAATTGGGCCTTAACTAAGAAACCGATGGCTGCAAATGGATTTGAGAGGAGGGATAGAGGAGGAGGGTTTTCGGATTTGCCGTCTTCGAAAGCCGACGAATCAGAGAGTTGGATTTCCAATAAGAGCAATGTTCCATCGGATGTGCGGAGATTTGGGACTAACGGCGGATTTGAAAGAGAGCGACGAGGAGGTTTTCCGACGGGCGGCGGAGGCGGTGCAGATTTAGAAAATTGGGGGAGGAAGAAAGAGGAAGGTAATAATGGTGGCAGCGTTGCGGGTAGGCCGAAGCTTAATTTGCAGCCACGGACACTGCCAGTGAGCGATGGGAAGTCGTCAGAGATATCAGGGACGGGGCTGGGGGCGAGGTCGAAGAGTTCAAATCCATTCGGAGAGGCGAGGCCGAGGGAACAGGTTCTGGCTGAGAAAGGACATGACTGGAAAAAACTCGACGAGCAACTGGAGTCGAAGATCAAAGACGAGCAACCAGAGAAGGTAGAAAACTCTAACAGCACTTCCTTTGGAAAGAAGAGCTTTGGCCGGGCATCTGAGGACCGAACAGAGAGGAGCTGGAGGAAGCCAGAGTCGACATCGGAGTCACTGGAATCTCGTCCCGGCAGTGCGGAGGAGAATGAAAATGGAGATGTTGAAGGGGAGTGTGAAGGGGACGAAAATGGAGCTTGA

Coding sequence (CDS)

ATGGCGGCAATGGTATCTCCATGGGGAAAGCCAGGCGCGTGGGCCCTCGACTCGGAGGAACACGAAGCTCAACTTCTGAAAGAAGAGCAGGAGCAGCAGAAACAAGCGGAGCTCGGCCTGGATTTCCCCTCCCTCAACGACGCCTTGGCCACCAAGCAGAAGAAGAAGGGCCAAACTCTCTCGTTGGCGGAGTTCAACGCCTATGGCGGCAGCGGCACCAGATCTTCCGCTCACACCGCTGCGCCCCAGGGCCTCACGCATGAAGATCTCATGGTGCTTCCCACCGGCCCTCGCCAACGGACGGCGGAGGAGCTCGACCGGAACCGCCTCGGCGGTGGCTTTAGGAATTACGGTACGAATGGTTCGTATGAGAGGAATAATAGGTATTCGATTGGGGATGATTCGTCTAATTCGAAGTGGGGTTCGTCTAGGGTTTCTGATGAGAATCGGAGGAACAATGGCGGGGGTAATAGGGGGGATTCGGGTAGGGAAATTGCACCTTCTCGGGCTGATGAGGTCGATAATTGGGCCTTAACTAAGAAACCGATGGCTGCAAATGGATTTGAGAGGAGGGATAGAGGAGGAGGGTTTTCGGATTTGCCGTCTTCGAAAGCCGACGAATCAGAGAGTTGGATTTCCAATAAGAGCAATGTTCCATCGGATGTGCGGAGATTTGGGACTAACGGCGGATTTGAAAGAGAGCGACGAGGAGGTTTTCCGACGGGCGGCGGAGGCGGTGCAGATTTAGAAAATTGGGGGAGGAAGAAAGAGGAAGGTAATAATGGTGGCAGCGTTGCGGGTAGGCCGAAGCTTAATTTGCAGCCACGGACACTGCCAGTGAGCGATGGGAAGTCGTCAGAGATATCAGGGACGGGGCTGGGGGCGAGGTCGAAGAGTTCAAATCCATTCGGAGAGGCGAGGCCGAGGGAACAGGTTCTGGCTGAGAAAGGACATGACTGGAAAAAACTCGACGAGCAACTGGAGTCGAAGATCAAAGACGAGCAACCAGAGAAGGTAGAAAACTCTAACAGCACTTCCTTTGGAAAGAAGAGCTTTGGCCGGGCATCTGAGGACCGAACAGAGAGGAGCTGGAGGAAGCCAGAGTCGACATCGGAGTCACTGGAATCTCGTCCCGGCAGTGCGGAGGAGAATGAAAATGGAGATGTTGAAGGGGAGTGTGAAGGGGACGAAAATGGAGCTTGA

Protein sequence

MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTKKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFPTGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRTERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA
Homology
BLAST of Moc04g04950 vs. NCBI nr
Match: XP_022135918.1 (eukaryotic translation initiation factor 4B3-like [Momordica charantia])

HSP 1 Score: 769.2 bits (1985), Expect = 1.7e-218
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL 60
           MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL
Sbjct: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN 120
           SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN
Sbjct: 61  SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN 120

Query: 121 GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK 180
           GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK
Sbjct: 121 GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK 180

Query: 181 KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP 240
           KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP
Sbjct: 181 KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP 240

Query: 241 TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS 300
           TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS
Sbjct: 241 TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS 300

Query: 301 NPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRT 360
           NPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRT
Sbjct: 301 NPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRT 360

Query: 361 ERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA 401
           ERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA
Sbjct: 361 ERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA 400

BLAST of Moc04g04950 vs. NCBI nr
Match: XP_038887904.1 (eukaryotic translation initiation factor 4B3-like isoform X1 [Benincasa hispida])

HSP 1 Score: 590.9 bits (1522), Expect = 8.3e-165
Identity = 330/406 (81.28%), Postives = 346/406 (85.22%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL 60
           MAAMVSPWGKPGAWALDSEEHEAQLLK EQE QKQAEL LDFPSLNDALATKQKKKGQTL
Sbjct: 1   MAAMVSPWGKPGAWALDSEEHEAQLLK-EQELQKQAELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN 120
           SLAEFN YG +G+RSS+     Q L HEDLMVLPTGPRQRTAEELDRNRLGGGFRNYG+N
Sbjct: 61  SLAEFNTYGSTGSRSSS-----QSLRHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGSN 120

Query: 121 GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK 180
           GSYERNNRYS GDDSSNSKWGSSRVSDENRRN  G NR DS +E   SRADEVDNWALTK
Sbjct: 121 GSYERNNRYSNGDDSSNSKWGSSRVSDENRRNGNGSNRADSSKEFTSSRADEVDNWALTK 180

Query: 181 KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP 240
           KPMA NGFERR+RG GFSD  SSKADESESW+SNK+NVPSD RRFG NGGF+RERRGGF 
Sbjct: 181 KPMAGNGFERRERGVGFSDSQSSKADESESWVSNKTNVPSDGRRFGANGGFDRERRGGF- 240

Query: 241 TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS 300
           T  GGGAD +NWGRKKEEGN GG +  RPKLNLQPRTLPVSDGKSSEISGT   ARSKSS
Sbjct: 241 TASGGGADSDNWGRKKEEGNTGGVIGSRPKLNLQPRTLPVSDGKSSEISGT--AARSKSS 300

Query: 301 NPFGEARPREQVLAEKGHDWKKLDEQLESKIKD-EQPEKVENSNSTSFGKKSFG----RA 360
           NPFG ARPRE+VLAEKGHDWKKLDEQLESKIK+    EK E SN+ SFGKKSFG    RA
Sbjct: 301 NPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNAISFGKKSFGNGKSRA 360

Query: 361 SEDRTERSWRKPES-TSESLESRPGSAEENENGDVEGECEGDENGA 401
            EDRTE+SWRKPES   ESLESRP S EENE  D EGE +GDENGA
Sbjct: 361 GEDRTEKSWRKPESPLQESLESRPDSGEENEIRDCEGERDGDENGA 397

BLAST of Moc04g04950 vs. NCBI nr
Match: XP_038887905.1 (eukaryotic translation initiation factor 4B3-like isoform X2 [Benincasa hispida])

HSP 1 Score: 570.9 bits (1470), Expect = 8.9e-159
Identity = 316/386 (81.87%), Postives = 331/386 (85.75%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL 60
           MAAMVSPWGKPGAWALDSEEHEAQLLK EQE QKQAEL LDFPSLNDALATKQKKKGQTL
Sbjct: 1   MAAMVSPWGKPGAWALDSEEHEAQLLK-EQELQKQAELSLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN 120
           SLAEFN YG +G+RSS+     Q L HEDLMVLPTGPRQRTAEELDRNRLGGGFRNYG+N
Sbjct: 61  SLAEFNTYGSTGSRSSS-----QSLRHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGSN 120

Query: 121 GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK 180
           GSYERNNRYS GDDSSNSKWGSSRVSDENRRN  G NR DS +E   SRADEVDNWALTK
Sbjct: 121 GSYERNNRYSNGDDSSNSKWGSSRVSDENRRNGNGSNRADSSKEFTSSRADEVDNWALTK 180

Query: 181 KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP 240
           KPMA NGFERR+RG GFSD  SSKADESESW+SNK+NVPSD RRFG NGGF+RERRGGF 
Sbjct: 181 KPMAGNGFERRERGVGFSDSQSSKADESESWVSNKTNVPSDGRRFGANGGFDRERRGGF- 240

Query: 241 TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS 300
           T  GGGAD +NWGRKKEEGN GG +  RPKLNLQPRTLPVSDGKSSEISGT   ARSKSS
Sbjct: 241 TASGGGADSDNWGRKKEEGNTGGVIGSRPKLNLQPRTLPVSDGKSSEISGT--AARSKSS 300

Query: 301 NPFGEARPREQVLAEKGHDWKKLDEQLESKIKD-EQPEKVENSNSTSFGKKSFG----RA 360
           NPFG ARPRE+VLAEKGHDWKKLDEQLESKIK+    EK E SN+ SFGKKSFG    RA
Sbjct: 301 NPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNAISFGKKSFGNGKSRA 360

Query: 361 SEDRTERSWRKPES-TSESLESRPGS 381
            EDRTE+SWRKPES   ESLESRP S
Sbjct: 361 GEDRTEKSWRKPESPLQESLESRPDS 377

BLAST of Moc04g04950 vs. NCBI nr
Match: XP_022928335.1 (eukaryotic translation initiation factor 4B3 isoform X2 [Cucurbita moschata])

HSP 1 Score: 558.9 bits (1439), Expect = 3.5e-155
Identity = 312/404 (77.23%), Postives = 340/404 (84.16%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQE------QQKQAELGLDFPSLNDALATKQK 60
           M AM SPWGKPGAWALDSEEHEA+LLKEEQE      QQK+AEL LDFPSLNDALATKQK
Sbjct: 1   MPAMASPWGKPGAWALDSEEHEAELLKEEQEQQQQQQQQKKAELSLDFPSLNDALATKQK 60

Query: 61  KKGQTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGF 120
           KKGQTLSLAEF A+ GSG++ S+     QG THED++VLPTGPR+RTAEELDRN+LGGGF
Sbjct: 61  KKGQTLSLAEFTAHAGSGSKYSS-----QGRTHEDVLVLPTGPRERTAEELDRNKLGGGF 120

Query: 121 RNYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVD 180
           RNYG+NGSYER+NRYS GDDSSNSKWGSSRVSDENRRN+GG NRGDS +E  PSRADEVD
Sbjct: 121 RNYGSNGSYERSNRYSNGDDSSNSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVD 180

Query: 181 NWALTKKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERE 240
           NWA TKKPMA NGFERRDRG GFSD  SSKADESESW+SNKSNVPSD RRFG NGGF+RE
Sbjct: 181 NWASTKKPMAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRE 240

Query: 241 RRGGFPTGGGGGADLENWGRKKEEGNNGGSVAG--RPKLNLQPRTLPVSDGKSSEISGTG 300
           RRGGFP   GGGAD + WGRKK+EGN+GG   G  RPKLNLQPRTLPV+D KSSEISGT 
Sbjct: 241 RRGGFP-ASGGGADSDTWGRKKDEGNSGGGGGGGSRPKLNLQPRTLPVNDVKSSEISGT- 300

Query: 301 LGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSF 360
             ARSKSSNPFGEARPRE+VLAEKGHDWKKLDE+LE KIK+   EK E SN  + GKKSF
Sbjct: 301 -TARSKSSNPFGEARPREEVLAEKGHDWKKLDEELELKIKEVSMEKTEKSN--AIGKKSF 360

Query: 361 G----RASEDRTERSWRKPES-TSESLESRPGSAEENENGDVEG 392
           G    RA+EDRTERSWRKPES   ES ESRP S E+NENG+ +G
Sbjct: 361 GNGTTRAAEDRTERSWRKPESPPEESPESRPDSVEDNENGEEDG 394

BLAST of Moc04g04950 vs. NCBI nr
Match: XP_022989048.1 (eukaryotic translation initiation factor 4B3-like [Cucurbita maxima])

HSP 1 Score: 558.9 bits (1439), Expect = 3.5e-155
Identity = 317/429 (73.89%), Postives = 348/429 (81.12%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQE---QQKQAELGLDFPSLNDALATKQKKKG 60
           M AM SPWGKPGAWALDSEEHEA+LLKEEQE   QQK+AEL LDFPSLNDALATKQKKKG
Sbjct: 1   MPAMASPWGKPGAWALDSEEHEAELLKEEQEQEQQQKKAELSLDFPSLNDALATKQKKKG 60

Query: 61  QTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNY 120
           QTLSLAEF A+GGSG++ S+     QG THED++VLPTGPR+RTAEELDRN+LGGGFRNY
Sbjct: 61  QTLSLAEFTAHGGSGSKYSS-----QGRTHEDVLVLPTGPRERTAEELDRNKLGGGFRNY 120

Query: 121 GTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWA 180
           G+NGSYER+NRYS GDDSS+SKWGSSRVSDENRRN+GG NRGDS +E  PSRADEVDNWA
Sbjct: 121 GSNGSYERSNRYSNGDDSSSSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVDNWA 180

Query: 181 LTKKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRG 240
            TKKPMA NGFERRDRG GFSD  SSKADESESW+SNKSNVPSD RRFG NGGF+RERRG
Sbjct: 181 STKKPMAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRERRG 240

Query: 241 GFPT--------------------GGGGGADLENWGRKKEEGNNGGSVAG-RPKLNLQPR 300
           GF                      GGGGGAD + WGRKK+EGN+GG   G RPKLNLQPR
Sbjct: 241 GFTASGGGADSDTWGRKKDEGNNGGGGGGADSDTWGRKKDEGNSGGGGGGSRPKLNLQPR 300

Query: 301 TLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQP 360
           TLPV+D KSSEISGT   ARSKSSNPFGEARPRE+VLAEKGHDWKKLDE+LE KIK+   
Sbjct: 301 TLPVNDVKSSEISGT--TARSKSSNPFGEARPREEVLAEKGHDWKKLDEELEMKIKELSM 360

Query: 361 EKVENSNSTSFGKKSF----GRASEDRTERSWRKPES-TSESLESRPGSAEENENGDVEG 401
           EK E SN  + GKKSF    G A+EDRTERSWRKPES   ES ESRP S E+NENGD EG
Sbjct: 361 EKTEKSN--AIGKKSFGDGTGHAAEDRTERSWRKPESPPEESPESRPDSVEDNENGDGEG 420

BLAST of Moc04g04950 vs. ExPASy Swiss-Prot
Match: Q9SZP8 (Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana OX=3702 GN=EIF4B3 PE=1 SV=1)

HSP 1 Score: 282.7 bits (722), Expect = 6.4e-75
Identity = 201/432 (46.53%), Postives = 263/432 (60.88%), Query Frame = 0

Query: 2   AAMVSPWGKPGAWALDSEEHEAQLLKEEQ--EQQKQAELGLDFPSLNDALATK-QKKKGQ 61
           AA+ S W KPGAWAL++EEHEA+L ++     Q+  AE   DFPSL  A  TK +KKKGQ
Sbjct: 3   AAVSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKKGQ 62

Query: 62  TLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYG 121
           T+SLAEF  YG +  + +  T   + LT  +L+ LPTGPR+R+AEELDR++LGGGFR+YG
Sbjct: 63  TISLAEFATYGTAKAKPAPQT---ERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG 122

Query: 122 TNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRG-DSGREIAPSRADEVDNWA 181
                    RY  GD+SS+S+WGSSRVS++  R  GG NR  +  R+  PSRADE DNWA
Sbjct: 123 -------GGRY--GDESSSSRWGSSRVSEDGERRGGGFNRDREPSRDSGPSRADEDDNWA 182

Query: 182 LTKKPMAANGFERRDRGGG---FSDLPSSKADESESWISNKSNVPSDVRRFGTN---GGF 241
             KKP++ NGFERR+RG G   F     SKADE +SW+S K   PS+ RRF ++   GG 
Sbjct: 183 AAKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTK---PSEPRRFVSSNGGGGD 242

Query: 242 ERERRGGFPT---------GGGGGADLENWGRKKEE-----GNNGGSVAGRPKLNLQPRT 301
             E+RG F +         GGGGG++ + WGR++EE     G+   S   RP+L LQPRT
Sbjct: 243 RFEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQPRT 302

Query: 302 LPVS--DGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQ 361
           LPV+  +    E     +  + K +NPFG ARPRE+VLAEKG DWK++DE+LE++   + 
Sbjct: 303 LPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKLKDI 362

Query: 362 PEKVENSNSTSFGKKSF----GRASEDRTERSWRKPESTSE--SLESRP----GSAEENE 398
              +E  N  S GK  F    GR  E+R ERSWRK    SE  + E  P       EE E
Sbjct: 363 AAAMEKPNEKSTGKMGFGLGNGRKDEERIERSWRKSTEHSEEDAQEEEPAVEGAKKEETE 419

BLAST of Moc04g04950 vs. ExPASy Swiss-Prot
Match: Q9AUJ7 (Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum OX=4565 GN=EIF4B PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 5.8e-36
Identity = 140/387 (36.18%), Postives = 185/387 (47.80%), Query Frame = 0

Query: 4   MVSPWGKPGAWALDS----EEHEAQLLKEEQEQQKQAELGLDFPSLNDALAT----KQKK 63
           M  PWG  GAWALD+    EE E        +    A     FPSL +A+      ++KK
Sbjct: 1   MAKPWGGVGAWALDAEREDEEREHAAAFPAPDPPAAAGGAASFPSLKEAVVAGGGKQKKK 60

Query: 64  KGQTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFR 123
           KG TLSL+EF  YG +G       A P+GLT +++M+LPTGPR+R+ +ELDR+R   GFR
Sbjct: 61  KGTTLSLSEFTTYGAAGAPRRVAPAEPKGLTPQEMMMLPTGPRERSEDELDRSR---GFR 120

Query: 124 NYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDN 183
           +YG     +R  R    DD       SSR SD +                 PSRADE DN
Sbjct: 121 SYGG----DREPRGGGFDDDRR----SSRDSDLD----------------MPSRADESDN 180

Query: 184 WALTKK-PMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERE 243
           W   K    A     RRDR  G S  P  ++D+ ++W  +K  +PS     GT GGF   
Sbjct: 181 WGKNKSFSPAPTDSGRRDRLSGPS--PLGRSDDIDNWSRDKKPLPSRYPSLGTGGGFRES 240

Query: 244 RRGGFPTGGGGG---------------ADLENWGRKKEEG---NNGGSVAGRPKLNLQPR 303
             GGF    GGG               +D + W R        NNG     RP+LNL P 
Sbjct: 241 SGGGFRESSGGGFRESSGGGFRDSPGPSDSDRWVRGAVPAPMTNNG----DRPRLNLNP- 300

Query: 304 TLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIK---- 358
             P  D  ++ +    + ARS+ S PFG A+PRE+VLAEKG DW+K++ ++E K      
Sbjct: 301 --PKRDPSATPVPAAEV-ARSRPS-PFGAAKPREEVLAEKGLDWRKMEGEIEKKTSRPTS 349

BLAST of Moc04g04950 vs. ExPASy Swiss-Prot
Match: Q9SAD7 (Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana OX=3702 GN=EIF4B2 PE=1 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 6.4e-27
Identity = 126/379 (33.25%), Postives = 171/379 (45.12%), Query Frame = 0

Query: 4   MVSPWGKPGAWALDSEEHEAQLLKEEQ--EQQKQAELGLDFPSLNDALATKQKKKGQTLS 63
           M  PWG  GAWA      EA+   EEQ  E    A     FPSL +A   K  KK + ++
Sbjct: 1   MSKPWGGIGAWA-----DEAERADEEQAAEATATAADSQSFPSLKEAATAKSSKKKKKMT 60

Query: 64  LAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTNG 123
           L+EF       T+ +    +  GLT E ++ LPTGPRQR+ +E+   RLGGGF +YG   
Sbjct: 61  LSEF-------TKGAYTAPSSAGLTREQMLQLPTGPRQRSEDEMQPGRLGGGFSSYGGGR 120

Query: 124 SYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGG---GNRGDSGREI---APSRADEVDN 183
           S     R S   + S+  WG        RR+ GG     RG + R       SRADE D+
Sbjct: 121 SSGPPGRMSRDREDSDGSWGG---GGGGRRSYGGFDDDQRGSNSRVSDFPQVSRADEDDD 180

Query: 184 WALTKKPM--------------------AANGFERRDRGGGFSDLPS------SKADESE 243
           W   KK +                       G      GGG +   S      SKADE +
Sbjct: 181 WGKGKKSLPSFDQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSKADEVD 240

Query: 244 SWISNKSNVPSDVRRFGTNGGF-ERERRGGFPTGGGGGADLENWGRKKEEGNNGGSVAGR 303
           +W + K+   +    F  +G   +R  RG  P+GGG           +EE         R
Sbjct: 241 NWAAGKAKSSTFGSGFRESGPEPDRWARGVLPSGGG----------VQEE---------R 300

Query: 304 PKLNLQPRTLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLE 347
            +L  +PR         +E+S T    ++   +PFG ARPREQVLAEKG DWKKLD  +E
Sbjct: 301 RRLVFEPRK------ADTEVSETPTAVKTSKPSPFGAARPREQVLAEKGLDWKKLDSDIE 339

BLAST of Moc04g04950 vs. ExPASy Swiss-Prot
Match: Q9LIN5 (Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana OX=3702 GN=EIF4B1 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.7e-25
Identity = 127/377 (33.69%), Postives = 170/377 (45.09%), Query Frame = 0

Query: 4   MVSPWG--KPGAWALDSEEHEAQLLKEEQEQQKQAELG--LDFPSLNDALA----TKQKK 63
           M   WG    GAWA      EA+   EEQ  +  A       FPSL +A A    + + +
Sbjct: 1   MSKAWGGIGIGAWA-----DEAERADEEQAAEATAATADTQSFPSLREAAAATATSGKSR 60

Query: 64  KGQTLSLAEF--NAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGG 123
           K + +SL+EF   AY   G R+S       GLT ++++ LPTGPRQR+ EE+   RLGGG
Sbjct: 61  KMKKMSLSEFTTGAYTAPGGRNSV------GLTQQEILQLPTGPRQRSEEEMQPGRLGGG 120

Query: 124 FRNYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGG----NRGDSGREI---A 183
           F +YG         R     D S+  W S       RR  GGG     RG+  R      
Sbjct: 121 FSSYGGRS----GGRIGRDRDDSDGSW-SGGGGGGGRRPYGGGFDDDRRGNQSRVSDFPQ 180

Query: 184 PSRADEVDNWALTKKPMAANGFERRDR----GGGFSDLPSSKADESESWISNKSNVPSDV 243
           PSRADEVD+W   KKP+ +    R+ R    GGGF                         
Sbjct: 181 PSRADEVDDWGKEKKPLPSFDQGRQGRYSGDGGGFG------------------------ 240

Query: 244 RRFGTNGGFERERRGGFPTGGGGGA----DLENWGRKKEEGNNGGSVAG----------R 303
              G   GF     GG   GGGGG     D++NWG  K +     S  G          R
Sbjct: 241 ---GGGSGF-----GGGGGGGGGGLSRADDVDNWGAGKRQAPVRSSTFGSSFGDSGQEER 300

Query: 304 PKLNLQPRTLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLE 346
            +L L+PR +     ++  +       ++   NPFG ARPRE VLAEKG DWKK+D ++E
Sbjct: 301 RRLVLEPRKVESGGSETPPVV-----EKTSKPNPFGAARPREDVLAEKGLDWKKIDSEIE 324

BLAST of Moc04g04950 vs. ExPASy TrEMBL
Match: A0A6J1C673 (eukaryotic translation initiation factor 4B3-like OS=Momordica charantia OX=3673 GN=LOC111007756 PE=4 SV=1)

HSP 1 Score: 769.2 bits (1985), Expect = 8.3e-219
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL 60
           MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL
Sbjct: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQTL 60

Query: 61  SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN 120
           SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN
Sbjct: 61  SLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTN 120

Query: 121 GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK 180
           GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK
Sbjct: 121 GSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALTK 180

Query: 181 KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP 240
           KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP
Sbjct: 181 KPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGFP 240

Query: 241 TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS 300
           TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS
Sbjct: 241 TGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKSS 300

Query: 301 NPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRT 360
           NPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRT
Sbjct: 301 NPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSFGRASEDRT 360

Query: 361 ERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA 401
           ERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA
Sbjct: 361 ERSWRKPESTSESLESRPGSAEENENGDVEGECEGDENGA 400

BLAST of Moc04g04950 vs. ExPASy TrEMBL
Match: A0A6J1JP41 (eukaryotic translation initiation factor 4B3-like OS=Cucurbita maxima OX=3661 GN=LOC111486238 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.7e-155
Identity = 317/429 (73.89%), Postives = 348/429 (81.12%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQE---QQKQAELGLDFPSLNDALATKQKKKG 60
           M AM SPWGKPGAWALDSEEHEA+LLKEEQE   QQK+AEL LDFPSLNDALATKQKKKG
Sbjct: 1   MPAMASPWGKPGAWALDSEEHEAELLKEEQEQEQQQKKAELSLDFPSLNDALATKQKKKG 60

Query: 61  QTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNY 120
           QTLSLAEF A+GGSG++ S+     QG THED++VLPTGPR+RTAEELDRN+LGGGFRNY
Sbjct: 61  QTLSLAEFTAHGGSGSKYSS-----QGRTHEDVLVLPTGPRERTAEELDRNKLGGGFRNY 120

Query: 121 GTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWA 180
           G+NGSYER+NRYS GDDSS+SKWGSSRVSDENRRN+GG NRGDS +E  PSRADEVDNWA
Sbjct: 121 GSNGSYERSNRYSNGDDSSSSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVDNWA 180

Query: 181 LTKKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRG 240
            TKKPMA NGFERRDRG GFSD  SSKADESESW+SNKSNVPSD RRFG NGGF+RERRG
Sbjct: 181 STKKPMAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRERRG 240

Query: 241 GFPT--------------------GGGGGADLENWGRKKEEGNNGGSVAG-RPKLNLQPR 300
           GF                      GGGGGAD + WGRKK+EGN+GG   G RPKLNLQPR
Sbjct: 241 GFTASGGGADSDTWGRKKDEGNNGGGGGGADSDTWGRKKDEGNSGGGGGGSRPKLNLQPR 300

Query: 301 TLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQP 360
           TLPV+D KSSEISGT   ARSKSSNPFGEARPRE+VLAEKGHDWKKLDE+LE KIK+   
Sbjct: 301 TLPVNDVKSSEISGT--TARSKSSNPFGEARPREEVLAEKGHDWKKLDEELEMKIKELSM 360

Query: 361 EKVENSNSTSFGKKSF----GRASEDRTERSWRKPES-TSESLESRPGSAEENENGDVEG 401
           EK E SN  + GKKSF    G A+EDRTERSWRKPES   ES ESRP S E+NENGD EG
Sbjct: 361 EKTEKSN--AIGKKSFGDGTGHAAEDRTERSWRKPESPPEESPESRPDSVEDNENGDGEG 420

BLAST of Moc04g04950 vs. ExPASy TrEMBL
Match: A0A6J1ERF5 (eukaryotic translation initiation factor 4B3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111435193 PE=4 SV=1)

HSP 1 Score: 558.9 bits (1439), Expect = 1.7e-155
Identity = 312/404 (77.23%), Postives = 340/404 (84.16%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQE------QQKQAELGLDFPSLNDALATKQK 60
           M AM SPWGKPGAWALDSEEHEA+LLKEEQE      QQK+AEL LDFPSLNDALATKQK
Sbjct: 1   MPAMASPWGKPGAWALDSEEHEAELLKEEQEQQQQQQQQKKAELSLDFPSLNDALATKQK 60

Query: 61  KKGQTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGF 120
           KKGQTLSLAEF A+ GSG++ S+     QG THED++VLPTGPR+RTAEELDRN+LGGGF
Sbjct: 61  KKGQTLSLAEFTAHAGSGSKYSS-----QGRTHEDVLVLPTGPRERTAEELDRNKLGGGF 120

Query: 121 RNYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVD 180
           RNYG+NGSYER+NRYS GDDSSNSKWGSSRVSDENRRN+GG NRGDS +E  PSRADEVD
Sbjct: 121 RNYGSNGSYERSNRYSNGDDSSNSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVD 180

Query: 181 NWALTKKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERE 240
           NWA TKKPMA NGFERRDRG GFSD  SSKADESESW+SNKSNVPSD RRFG NGGF+RE
Sbjct: 181 NWASTKKPMAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRE 240

Query: 241 RRGGFPTGGGGGADLENWGRKKEEGNNGGSVAG--RPKLNLQPRTLPVSDGKSSEISGTG 300
           RRGGFP   GGGAD + WGRKK+EGN+GG   G  RPKLNLQPRTLPV+D KSSEISGT 
Sbjct: 241 RRGGFP-ASGGGADSDTWGRKKDEGNSGGGGGGGSRPKLNLQPRTLPVNDVKSSEISGT- 300

Query: 301 LGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQPEKVENSNSTSFGKKSF 360
             ARSKSSNPFGEARPRE+VLAEKGHDWKKLDE+LE KIK+   EK E SN  + GKKSF
Sbjct: 301 -TARSKSSNPFGEARPREEVLAEKGHDWKKLDEELELKIKEVSMEKTEKSN--AIGKKSF 360

Query: 361 G----RASEDRTERSWRKPES-TSESLESRPGSAEENENGDVEG 392
           G    RA+EDRTERSWRKPES   ES ESRP S E+NENG+ +G
Sbjct: 361 GNGTTRAAEDRTERSWRKPESPPEESPESRPDSVEDNENGEEDG 394

BLAST of Moc04g04950 vs. ExPASy TrEMBL
Match: A0A6J1EKJ0 (eukaryotic translation initiation factor 4B3 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111435193 PE=4 SV=1)

HSP 1 Score: 555.8 bits (1431), Expect = 1.4e-154
Identity = 314/425 (73.88%), Postives = 342/425 (80.47%), Query Frame = 0

Query: 1   MAAMVSPWGKPGAWALDSEEHEAQLLKEEQE------QQKQAELGLDFPSLNDALATKQK 60
           M AM SPWGKPGAWALDSEEHEA+LLKEEQE      QQK+AEL LDFPSLNDALATKQK
Sbjct: 1   MPAMASPWGKPGAWALDSEEHEAELLKEEQEQQQQQQQQKKAELSLDFPSLNDALATKQK 60

Query: 61  KKGQTLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGF 120
           KKGQTLSLAEF A+ GSG++ S+     QG THED++VLPTGPR+RTAEELDRN+LGGGF
Sbjct: 61  KKGQTLSLAEFTAHAGSGSKYSS-----QGRTHEDVLVLPTGPRERTAEELDRNKLGGGF 120

Query: 121 RNYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVD 180
           RNYG+NGSYER+NRYS GDDSSNSKWGSSRVSDENRRN+GG NRGDS +E  PSRADEVD
Sbjct: 121 RNYGSNGSYERSNRYSNGDDSSNSKWGSSRVSDENRRNDGGFNRGDSSKEFVPSRADEVD 180

Query: 181 NWALTKKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERE 240
           NWA TKKPMA NGFERRDRG GFSD  SSKADESESW+SNKSNVPSD RRFG NGGF+RE
Sbjct: 181 NWASTKKPMAGNGFERRDRGLGFSDSQSSKADESESWVSNKSNVPSDARRFGANGGFDRE 240

Query: 241 RRGGFPT---------------------GGGGGADLENWGRKKEEGNNGGSVAG--RPKL 300
           RRGGFP                      GGGGGAD + WGRKK+EGN+GG   G  RPKL
Sbjct: 241 RRGGFPASGGGADSDTWGRKKDEGNIGGGGGGGADSDTWGRKKDEGNSGGGGGGGSRPKL 300

Query: 301 NLQPRTLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKI 360
           NLQPRTLPV+D KSSEISGT   ARSKSSNPFGEARPRE+VLAEKGHDWKKLDE+LE KI
Sbjct: 301 NLQPRTLPVNDVKSSEISGT--TARSKSSNPFGEARPREEVLAEKGHDWKKLDEELELKI 360

Query: 361 KDEQPEKVENSNSTSFGKKSFG----RASEDRTERSWRKPES-TSESLESRPGSAEENEN 392
           K+   EK E SN  + GKKSFG    RA+EDRTERSWRKPES   ES ESRP S E+NEN
Sbjct: 361 KEVSMEKTEKSN--AIGKKSFGNGTTRAAEDRTERSWRKPESPPEESPESRPDSVEDNEN 416

BLAST of Moc04g04950 vs. ExPASy TrEMBL
Match: A0A5A7U3A1 (Eukaryotic translation initiation factor 4B3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00800 PE=4 SV=1)

HSP 1 Score: 536.6 bits (1381), Expect = 9.0e-149
Identity = 312/410 (76.10%), Postives = 332/410 (80.98%), Query Frame = 0

Query: 1   MAAMV-SPWGKPGAWALDSEEHEAQLLKEEQEQQKQAELGLDFPSLNDALATKQKKKGQT 60
           MAAMV SPWGKPGAWALDSEEHEAQLLK EQEQQKQ EL LDFPSLNDALATKQKKKGQT
Sbjct: 1   MAAMVSSPWGKPGAWALDSEEHEAQLLK-EQEQQKQPELSLDFPSLNDALATKQKKKGQT 60

Query: 61  LSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGT 120
           LSLAEFN YG +G+RS            ED MVLPTGPRQRTAEELDRNRLGGGFRNYG+
Sbjct: 61  LSLAEFNTYGTTGSRS------------EDFMVLPTGPRQRTAEELDRNRLGGGFRNYGS 120

Query: 121 NGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRGDSGREIAPSRADEVDNWALT 180
           NGSYERNNRYS  DDSSNSKWGSSRVSDENRR++ G NR DS +E   SRADEVDNWALT
Sbjct: 121 NGSYERNNRYSNADDSSNSKWGSSRVSDENRRSSNGSNRADSSKEFTSSRADEVDNWALT 180

Query: 181 KKPMAANGFERRDRGGGFSDLPSSKADESESWISNKSNVPSDVRRFGTNGGFERERRGGF 240
           KKPMA NGFERR+RG GFSD  SSKADESESW+SNKSN+PS  RR      F+RERRGGF
Sbjct: 181 KKPMAGNGFERRERGLGFSDSQSSKADESESWVSNKSNIPSGGRR------FDRERRGGF 240

Query: 241 PTGGGGGADLENWGRKKEEGNNGGSVAGRPKLNLQPRTLPVSDGKSSEISGTGLGARSKS 300
            T  GGGAD +NWGRKKEEGN GG +  RPKL+LQPRTLPV DGKSSEIS T   ARSKS
Sbjct: 241 -TASGGGADSDNWGRKKEEGNAGGVIGSRPKLSLQPRTLPVGDGKSSEISAT--AARSKS 300

Query: 301 SNPFGEARPREQVLAEKGHDWKKLDEQLESKIKD-EQPEKVENSNSTSFGKKSFG----R 360
           SNPFG ARPRE+VLAEKGHDWKKLDEQLESKIK+    EK E SN+ SF K+SFG    R
Sbjct: 301 SNPFGAARPREEVLAEKGHDWKKLDEQLESKIKEVSVEEKTEKSNAISFRKRSFGNGNSR 360

Query: 361 ASEDRTERSWRKPES--TSESLESRPGSAEENENGDVEG--ECEGDENGA 401
           A+EDRTE+SWRKPES    ES ESRP S EEN NGD EG  E +GDENGA
Sbjct: 361 AAEDRTEKSWRKPESPPPQESPESRPESGEENVNGDCEGKHEHDGDENGA 388

BLAST of Moc04g04950 vs. TAIR 10
Match: AT4G38710.2 (glycine-rich protein )

HSP 1 Score: 285.0 bits (728), Expect = 9.1e-77
Identity = 198/421 (47.03%), Postives = 259/421 (61.52%), Query Frame = 0

Query: 2   AAMVSPWGKPGAWALDSEEHEAQLLKEEQ--EQQKQAELGLDFPSLNDALATK-QKKKGQ 61
           AA+ S W KPGAWAL++EEHEA+L ++     Q+  AE   DFPSL  A  TK +KKKGQ
Sbjct: 3   AAVSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKKGQ 62

Query: 62  TLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYG 121
           T+SLAEF  YG +  + +  T   + LT  +L+ LPTGPR+R+AEELDR++LGGGFR+YG
Sbjct: 63  TISLAEFATYGTAKAKPAPQT---ERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG 122

Query: 122 TNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRG-DSGREIAPSRADEVDNWA 181
                    RY  GD+SS+S+WGSSRVS++  R  GG NR  +  R+  PSRADE DNWA
Sbjct: 123 -------GGRY--GDESSSSRWGSSRVSEDGERRGGGFNRDREPSRDSGPSRADEDDNWA 182

Query: 182 LTKKPMAANGFERRDRGGG---FSDLPSSKADESESWISNKSNVPSDVRRFGTN---GGF 241
             KKP++ NGFERR+RG G   F     SKADE +SW+S K   PS+ RRF ++   GG 
Sbjct: 183 AAKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTK---PSEPRRFVSSNGGGGD 242

Query: 242 ERERRGGFPT---------GGGGGADLENWGRKKEE-----GNNGGSVAGRPKLNLQPRT 301
             E+RG F +         GGGGG++ + WGR++EE     G+   S   RP+L LQPRT
Sbjct: 243 RFEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQPRT 302

Query: 302 LPVS--DGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQ 361
           LPV+  +    E     +  + K +NPFG ARPRE+VLAEKG DWK++DE+LE++   + 
Sbjct: 303 LPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKLKDI 362

Query: 362 PEKVENSNSTSFGKKSF----GRASEDRTERSWRKPESTSESLESRPGSAEENENGDVEG 393
              +E  N  S GK  F    GR  E+R ERSWRK  S    +E    + E +E    E 
Sbjct: 363 AAAMEKPNEKSTGKMGFGLGNGRKDEERIERSWRKSFSLHSYMEVDVLNTEHSEEDAQEE 408

BLAST of Moc04g04950 vs. TAIR 10
Match: AT4G38710.1 (glycine-rich protein )

HSP 1 Score: 282.7 bits (722), Expect = 4.5e-76
Identity = 201/432 (46.53%), Postives = 263/432 (60.88%), Query Frame = 0

Query: 2   AAMVSPWGKPGAWALDSEEHEAQLLKEEQ--EQQKQAELGLDFPSLNDALATK-QKKKGQ 61
           AA+ S W KPGAWAL++EEHEA+L ++     Q+  AE   DFPSL  A  TK +KKKGQ
Sbjct: 3   AAVSSVWAKPGAWALEAEEHEAELKQQPSPTNQKSSAEDSSDFPSLAAAATTKTKKKKGQ 62

Query: 62  TLSLAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYG 121
           T+SLAEF  YG +  + +  T   + LT  +L+ LPTGPR+R+AEELDR++LGGGFR+YG
Sbjct: 63  TISLAEFATYGTAKAKPAPQT---ERLTQAELVALPTGPRERSAEELDRSKLGGGFRSYG 122

Query: 122 TNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGGNRG-DSGREIAPSRADEVDNWA 181
                    RY  GD+SS+S+WGSSRVS++  R  GG NR  +  R+  PSRADE DNWA
Sbjct: 123 -------GGRY--GDESSSSRWGSSRVSEDGERRGGGFNRDREPSRDSGPSRADEDDNWA 182

Query: 182 LTKKPMAANGFERRDRGGG---FSDLPSSKADESESWISNKSNVPSDVRRFGTN---GGF 241
             KKP++ NGFERR+RG G   F     SKADE +SW+S K   PS+ RRF ++   GG 
Sbjct: 183 AAKKPISGNGFERRERGSGGGFFESQSQSKADEVDSWVSTK---PSEPRRFVSSNGGGGD 242

Query: 242 ERERRGGFPT---------GGGGGADLENWGRKKEE-----GNNGGSVAGRPKLNLQPRT 301
             E+RG F +         GGGGG++ + WGR++EE     G+   S   RP+L LQPRT
Sbjct: 243 RFEKRGSFESLSRNRDSQYGGGGGSESDTWGRRREESGAANGSPPPSGGSRPRLVLQPRT 302

Query: 302 LPVS--DGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLESKIKDEQ 361
           LPV+  +    E     +  + K +NPFG ARPRE+VLAEKG DWK++DE+LE++   + 
Sbjct: 303 LPVAVVEVVKPESPVLVIVEKPKGANPFGNARPREEVLAEKGQDWKEIDEKLEAEKLKDI 362

Query: 362 PEKVENSNSTSFGKKSF----GRASEDRTERSWRKPESTSE--SLESRP----GSAEENE 398
              +E  N  S GK  F    GR  E+R ERSWRK    SE  + E  P       EE E
Sbjct: 363 AAAMEKPNEKSTGKMGFGLGNGRKDEERIERSWRKSTEHSEEDAQEEEPAVEGAKKEETE 419

BLAST of Moc04g04950 vs. TAIR 10
Match: AT1G13020.1 (eukaryotic initiation factor 4B2 )

HSP 1 Score: 123.2 bits (308), Expect = 4.6e-28
Identity = 126/379 (33.25%), Postives = 171/379 (45.12%), Query Frame = 0

Query: 4   MVSPWGKPGAWALDSEEHEAQLLKEEQ--EQQKQAELGLDFPSLNDALATKQKKKGQTLS 63
           M  PWG  GAWA      EA+   EEQ  E    A     FPSL +A   K  KK + ++
Sbjct: 1   MSKPWGGIGAWA-----DEAERADEEQAAEATATAADSQSFPSLKEAATAKSSKKKKKMT 60

Query: 64  LAEFNAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGGFRNYGTNG 123
           L+EF       T+ +    +  GLT E ++ LPTGPRQR+ +E+   RLGGGF +YG   
Sbjct: 61  LSEF-------TKGAYTAPSSAGLTREQMLQLPTGPRQRSEDEMQPGRLGGGFSSYGGGR 120

Query: 124 SYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGG---GNRGDSGREI---APSRADEVDN 183
           S     R S   + S+  WG        RR+ GG     RG + R       SRADE D+
Sbjct: 121 SSGPPGRMSRDREDSDGSWGG---GGGGRRSYGGFDDDQRGSNSRVSDFPQVSRADEDDD 180

Query: 184 WALTKKPM--------------------AANGFERRDRGGGFSDLPS------SKADESE 243
           W   KK +                       G      GGG +   S      SKADE +
Sbjct: 181 WGKGKKSLPSFDQGRQGSRYGGGGGSFGGGGGGGAGSYGGGGAGAGSGGGGGFSKADEVD 240

Query: 244 SWISNKSNVPSDVRRFGTNGGF-ERERRGGFPTGGGGGADLENWGRKKEEGNNGGSVAGR 303
           +W + K+   +    F  +G   +R  RG  P+GGG           +EE         R
Sbjct: 241 NWAAGKAKSSTFGSGFRESGPEPDRWARGVLPSGGG----------VQEE---------R 300

Query: 304 PKLNLQPRTLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLE 347
            +L  +PR         +E+S T    ++   +PFG ARPREQVLAEKG DWKKLD  +E
Sbjct: 301 RRLVFEPRK------ADTEVSETPTAVKTSKPSPFGAARPREQVLAEKGLDWKKLDSDIE 339

BLAST of Moc04g04950 vs. TAIR 10
Match: AT3G26400.1 (eukaryotic translation initiation factor 4B1 )

HSP 1 Score: 117.9 bits (294), Expect = 1.9e-26
Identity = 127/377 (33.69%), Postives = 170/377 (45.09%), Query Frame = 0

Query: 4   MVSPWG--KPGAWALDSEEHEAQLLKEEQEQQKQAELG--LDFPSLNDALA----TKQKK 63
           M   WG    GAWA      EA+   EEQ  +  A       FPSL +A A    + + +
Sbjct: 1   MSKAWGGIGIGAWA-----DEAERADEEQAAEATAATADTQSFPSLREAAAATATSGKSR 60

Query: 64  KGQTLSLAEF--NAYGGSGTRSSAHTAAPQGLTHEDLMVLPTGPRQRTAEELDRNRLGGG 123
           K + +SL+EF   AY   G R+S       GLT ++++ LPTGPRQR+ EE+   RLGGG
Sbjct: 61  KMKKMSLSEFTTGAYTAPGGRNSV------GLTQQEILQLPTGPRQRSEEEMQPGRLGGG 120

Query: 124 FRNYGTNGSYERNNRYSIGDDSSNSKWGSSRVSDENRRNNGGG----NRGDSGREI---A 183
           F +YG         R     D S+  W S       RR  GGG     RG+  R      
Sbjct: 121 FSSYGGRS----GGRIGRDRDDSDGSW-SGGGGGGGRRPYGGGFDDDRRGNQSRVSDFPQ 180

Query: 184 PSRADEVDNWALTKKPMAANGFERRDR----GGGFSDLPSSKADESESWISNKSNVPSDV 243
           PSRADEVD+W   KKP+ +    R+ R    GGGF                         
Sbjct: 181 PSRADEVDDWGKEKKPLPSFDQGRQGRYSGDGGGFG------------------------ 240

Query: 244 RRFGTNGGFERERRGGFPTGGGGGA----DLENWGRKKEEGNNGGSVAG----------R 303
              G   GF     GG   GGGGG     D++NWG  K +     S  G          R
Sbjct: 241 ---GGGSGF-----GGGGGGGGGGLSRADDVDNWGAGKRQAPVRSSTFGSSFGDSGQEER 300

Query: 304 PKLNLQPRTLPVSDGKSSEISGTGLGARSKSSNPFGEARPREQVLAEKGHDWKKLDEQLE 346
            +L L+PR +     ++  +       ++   NPFG ARPRE VLAEKG DWKK+D ++E
Sbjct: 301 RRLVLEPRKVESGGSETPPVV-----EKTSKPNPFGAARPREDVLAEKGLDWKKIDSEIE 324

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022135918.11.7e-218100.00eukaryotic translation initiation factor 4B3-like [Momordica charantia][more]
XP_038887904.18.3e-16581.28eukaryotic translation initiation factor 4B3-like isoform X1 [Benincasa hispida][more]
XP_038887905.18.9e-15981.87eukaryotic translation initiation factor 4B3-like isoform X2 [Benincasa hispida][more]
XP_022928335.13.5e-15577.23eukaryotic translation initiation factor 4B3 isoform X2 [Cucurbita moschata][more]
XP_022989048.13.5e-15573.89eukaryotic translation initiation factor 4B3-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SZP86.4e-7546.53Eukaryotic translation initiation factor 4B3 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9AUJ75.8e-3636.18Eukaryotic translation initiation factor 4B1 OS=Triticum aestivum OX=4565 GN=EIF... [more]
Q9SAD76.4e-2733.25Eukaryotic translation initiation factor 4B2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9LIN52.7e-2533.69Eukaryotic translation initiation factor 4B1 OS=Arabidopsis thaliana OX=3702 GN=... [more]
Match NameE-valueIdentityDescription
A0A6J1C6738.3e-219100.00eukaryotic translation initiation factor 4B3-like OS=Momordica charantia OX=3673... [more]
A0A6J1JP411.7e-15573.89eukaryotic translation initiation factor 4B3-like OS=Cucurbita maxima OX=3661 GN... [more]
A0A6J1ERF51.7e-15577.23eukaryotic translation initiation factor 4B3 isoform X2 OS=Cucurbita moschata OX... [more]
A0A6J1EKJ01.4e-15473.88eukaryotic translation initiation factor 4B3 isoform X1 OS=Cucurbita moschata OX... [more]
A0A5A7U3A19.0e-14976.10Eukaryotic translation initiation factor 4B3 OS=Cucumis melo var. makuwa OX=1194... [more]
Match NameE-valueIdentityDescription
AT4G38710.29.1e-7747.03glycine-rich protein [more]
AT4G38710.14.5e-7646.53glycine-rich protein [more]
AT1G13020.14.6e-2833.25eukaryotic initiation factor 4B2 [more]
AT3G26400.11.9e-2633.69eukaryotic translation initiation factor 4B1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 16..36
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..400
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 278..298
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 352..384
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 203..223
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 119..156
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 385..400
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 309..339
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 67..82
NoneNo IPR availablePANTHERPTHR32091:SF17EUKARYOTIC TRANSLATION INITIATION FACTOR 4B3coord: 1..372
IPR010433Plant specific eukaryotic initiation factor 4BPFAMPF06273eIF-4Bcoord: 4..352
e-value: 6.3E-57
score: 193.8
IPR010433Plant specific eukaryotic initiation factor 4BPANTHERPTHR32091EUKARYOTIC TRANSLATION INITIATION FACTOR 4Bcoord: 1..372

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g04950.1Moc04g04950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006413 translational initiation
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003743 translation initiation factor activity