Moc04g04700 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g04700
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	FAD_binding_3 domain-containing protein
Location	chr4: 3062965 .. 3064119 (+)
RNA-Seq Expression	Moc04g04700
Synteny	Moc04g04700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACATTACTCAAAACCAAGGTAAGTCCTGTGTTTTTACTCACGAGCATGGCCATGGATTTGAGAACAAGTTCATGTGGTTTTTCAGCAAAGGCCTTCGCTTCGGCGTCATGCCGTGTAGTGCCAACACGGTCTATTGGTTTATGACTTGGCGTCCTTCCAAACAAGGTTAGTCCCTCTCACTAAAAGAAATTATTCAACGTGGTCGTTTTACTGATAATTGTTGAATGTAAGTTCAAAATCTACCTGCCTCCTAATGATGGTGAGTGAGATATGAATTAAAAAACTTGCTTTTTTTAGTACAATATGTGAGGTGGGAGATCGAACTCACAAACTCTTAATTGGAGATATATGTCAATTACCGCTGATTATGCTCGCTTTGAAAAAAATATAGCTTTTAAATTGTCCAATACATTTAACACTATAAAATTATCAATCACATTGTTTCAGCATCGTTGAGTAATTTCTCCTCCTACCGGACTACAAATTCACTTTTAGCATTAACGATAGTGAGACAAGTTCATATAGAAATATTGTATTGGAATGATAAACAGAAAATGGCCAACTCTTGTTTGCCCTCACTCTTCTTAGTCACTCTATAACCACTCTATAAAAATCTAACTCAAACTCATGTTTAATGATACATATATCCTCTTGAAATGGGTTCAAACCATGGTGGTCACCTGGTTAGGATTTAATATCCTACGAATTACCATGTCAATCAAATGTAGTAGGTCAGACGGTTAGTCAGTGATTAATCGAGGTGTGCGCAAGCTGGTCCAGACATACGGATATGAAAAAAAAAAAAGAAACCCCTCTTAATATTGTTATAATGCTCATAGTATCTCCCTGTTGATATTCCTAATACATTGTGAAACTTGCAGAGGAGACTGAAGAGAACCCATCAAGACTGAAGCAACACCTTTTGAGCAAGCTTGGCAAGGTACCTGATCAAATAAGGGCTGTTGTTGAAGACACAGAAGTTGATTCTTTTGTGTCACTGCCATTGAGATATAGGCACCCTTGGCAGCTTGTCCGGAATAACTTTTCCCGCGGCAACGTTACCATCGCTGGCGATGCACTCCACCCGATGACCCCGGATCTCAGCCAAGGAGGTTGTTCTGCACTGGAAGACGGCGTCGTTCTCGCCTGA

mRNA sequence

ATGGAACATTACTCAAAACCAAGCAAAGGCCTTCGCTTCGGCGTCATGCCGTGTAGTGCCAACACGGTCTATTGGTTTATGACTTGGCGTCCTTCCAAACAAGAGGAGACTGAAGAGAACCCATCAAGACTGAAGCAACACCTTTTGAGCAAGCTTGGCAAGGTACCTGATCAAATAAGGGCTGTTGTTGAAGACACAGAAGTTGATTCTTTTGTGTCACTGCCATTGAGATATAGGCACCCTTGGCAGCTTGTCCGGAATAACTTTTCCCGCGGCAACGTTACCATCGCTGGCGATGCACTCCACCCGATGACCCCGGATCTCAGCCAAGGAGGTTGTTCTGCACTGGAAGACGGCGTCGTTCTCGCCTGA

Coding sequence (CDS)

ATGGAACATTACTCAAAACCAAGCAAAGGCCTTCGCTTCGGCGTCATGCCGTGTAGTGCCAACACGGTCTATTGGTTTATGACTTGGCGTCCTTCCAAACAAGAGGAGACTGAAGAGAACCCATCAAGACTGAAGCAACACCTTTTGAGCAAGCTTGGCAAGGTACCTGATCAAATAAGGGCTGTTGTTGAAGACACAGAAGTTGATTCTTTTGTGTCACTGCCATTGAGATATAGGCACCCTTGGCAGCTTGTCCGGAATAACTTTTCCCGCGGCAACGTTACCATCGCTGGCGATGCACTCCACCCGATGACCCCGGATCTCAGCCAAGGAGGTTGTTCTGCACTGGAAGACGGCGTCGTTCTCGCCTGA

Protein sequence

MEHYSKPSKGLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA

Homology

[Close]

BLAST of Moc04g04700 vs. NCBI nr
Match: XP_004144855.1 (monooxygenase 2 [Cucumis sativus] >KGN43264.1 hypothetical protein Csa_020371 [Cucumis sativus])

HSP 1 Score: 213.0 bits (541), Expect = 1.5e-51
Identity = 98/116 (84.48%), Postives = 107/116 (92.24%), Query Frame = 0


Query: 9   KGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDTE 68
           KGLRFGVMPC++NTVYWF TWRPSKQ EE EENP++LK+H+L KLGKVPDQ RAVVEDTE
Sbjct: 206 KGLRFGVMPCNSNTVYWFATWRPSKQEEEIEENPTKLKEHILRKLGKVPDQARAVVEDTE 265

Query: 69  VDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           VDSFVSLPLRYRHPW+LV NNFSR N+TIAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 266 VDSFVSLPLRYRHPWELVCNNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILA 321

[Close]

BLAST of Moc04g04700 vs. NCBI nr
Match: XP_038887729.1 (monooxygenase 2-like [Benincasa hispida])

HSP 1 Score: 211.1 bits (536), Expect = 5.5e-51
Identity = 98/117 (83.76%), Postives = 109/117 (93.16%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC+++TVYWF TWRPS+Q EE EENP RLKQH+LSKLGKVPDQIRAVVEDT
Sbjct: 202 SKGLRFGVMPCNSDTVYWFSTWRPSEQEEEIEENPVRLKQHILSKLGKVPDQIRAVVEDT 261

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           EVDSF+SLPLR+R PW+LV NNFSRGN++IAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 262 EVDSFMSLPLRFRRPWELVWNNFSRGNISIAGDALHSMTPDLGQGGCSALEDGVILA 318

[Close]

BLAST of Moc04g04700 vs. NCBI nr
Match: XP_008447940.1 (PREDICTED: FAD-dependent urate hydroxylase-like [Cucumis melo] >KAA0049767.1 FAD-dependent urate hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 209.1 bits (531), Expect = 2.1e-50
Identity = 97/117 (82.91%), Postives = 108/117 (92.31%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC++NTVYWF TW PSKQ EE EENP++LK+H+LSKLGKVPDQ RAVVEDT
Sbjct: 204 SKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAVVEDT 263

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VD+FVSLPLRYR PW+LV NNFSRGNV+IAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 264 QVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVILA 320

[Close]

BLAST of Moc04g04700 vs. NCBI nr
Match: TYK12110.1 (FAD-dependent urate hydroxylase-like [Cucumis melo var. makuwa])

HSP 1 Score: 209.1 bits (531), Expect = 2.1e-50
Identity = 97/117 (82.91%), Postives = 108/117 (92.31%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC++NTVYWF TW PSKQ EE EENP++LK+H+LSKLGKVPDQ RAVVEDT
Sbjct: 184 SKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAVVEDT 243

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VD+FVSLPLRYR PW+LV NNFSRGNV+IAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 244 QVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVILA 300

[Close]

BLAST of Moc04g04700 vs. NCBI nr
Match: XP_022968891.1 (monooxygenase 2-like, partial [Cucurbita maxima])

HSP 1 Score: 204.5 bits (519), Expect = 5.2e-49
Identity = 97/117 (82.91%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC+ANTVYWF TW  SKQ +E EENP RLKQH+LSKLGKVPD++RAVVED+
Sbjct: 225 SKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAVVEDS 284

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VDSFVSLPLR RHPW+LV N+FSRGNV IAGDALH MTPDL QGGCSALEDGVVLA
Sbjct: 285 KVDSFVSLPLRCRHPWELVWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLA 341

[Close]

BLAST of Moc04g04700 vs. ExPASy Swiss-Prot
Match: O81816 (Monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MO2 PE=2 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.5e-30
Identity = 60/114 (52.63%), Postives = 83/114 (72.81%), Query Frame = 0


Query: 10  GLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVD 69
           G+R G +PC  NTVYWF+T   S   + E N   LK+ +L+K+  +P+ I+ VVE T++D
Sbjct: 203 GVRSGFIPCDHNTVYWFLT-HTSTDIDEETNSEILKEFVLNKIKDLPENIKNVVETTDLD 262

Query: 70  SFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           S V   L+YR PW+L+ +N ++ NV +AGDALHPMTPD+ QGGCSA+EDGV+LA
Sbjct: 263 SMVMSQLKYRPPWELLWSNITKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILA 315

[Close]

BLAST of Moc04g04700 vs. ExPASy Swiss-Prot
Match: Q9FLC2 (Monooxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=MO3 PE=2 SV=1)

HSP 1 Score: 131.7 bits (330), Expect = 5.6e-30
Identity = 57/114 (50.00%), Postives = 84/114 (73.68%), Query Frame = 0


Query: 10  GLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVD 69
           G+R G + C  NTVYWF+T   +  +  ++N  ++KQ +L+K+  +PD I++++E T++D
Sbjct: 203 GVRSGFISCDQNTVYWFLT--HTSTDLDKKNHQKIKQFVLTKIKDLPDNIKSILETTDLD 262

Query: 70  SFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           S V  PL YR PW+L+  N ++ NV +AGDALHPMTPD+ QGGCSA+EDGV+LA
Sbjct: 263 SLVMNPLMYRPPWELLWANIAKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILA 314

[Close]

BLAST of Moc04g04700 vs. ExPASy Swiss-Prot
Match: O81815 (Monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MO1 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.4e-12
Identity = 39/110 (35.45%), Postives = 59/110 (53.64%), Query Frame = 0


Query: 14  GVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVDSFVS 73
           G +P + N V+WF+          ++  S +          + +  + +V+   V+S   
Sbjct: 203 GRLPLTDNQVFWFLVHMQDNNHNGKDQES-IANLCRKWADDLSEDWKEMVKICNVESLTL 262

Query: 74  LPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
             LRYR P +++   F RG VT+AGDA+H M P L+QGG +ALED VVLA
Sbjct: 263 THLRYRAPSEIMLGKFRRGTVTVAGDAMHVMGPFLAQGGSAALEDAVVLA 311

[Close]

BLAST of Moc04g04700 vs. ExPASy Swiss-Prot
Match: H1ZZA4 (Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 1.5e-11
Identity = 39/114 (34.21%), Postives = 59/114 (51.75%), Query Frame = 0


Query: 10  GLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVD 69
           G+RFGV+P      YWF T   +  E   ++P   +  LL +       I  ++E+T   
Sbjct: 203 GMRFGVVPIGEGQTYWFAT--ATAPEGGVDHPD-ARTELLQRFSGWHAPIPQLIENTPSS 262

Query: 70  SFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           + +   +  R P +     + +G   + GDA HPMTP++ QGGC A+ED VVLA
Sbjct: 263 AIMRTDIHDRVPIR----QWVQGRAVLLGDAAHPMTPNMGQGGCQAVEDAVVLA 309

[Close]

BLAST of Moc04g04700 vs. ExPASy Swiss-Prot
Match: Q6F6Y2 (FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1) OX=62977 GN=hpxO PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.3e-07
Identity = 33/115 (28.70%), Postives = 55/115 (47.83%), Query Frame = 0


Query: 9   KGLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEV 68
           +G R  +MP + N  Y+F+      +   E +  + K+ L S       Q++ ++E  + 
Sbjct: 201 EGKRASLMPVADNRFYFFLD--VPLEAGLENDKCKYKETLQSYFKGWCPQVQTLIERLDP 260

Query: 69  DSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
                + +    P+      F +G V + GDA H  TPD+ QGGC A+ED + LA
Sbjct: 261 QKTNRVEICDIEPFA----QFYKGRVVLVGDAAHSTTPDIGQGGCQAMEDAIYLA 309

[Close]

BLAST of Moc04g04700 vs. ExPASy TrEMBL
Match: A0A0A0K2K7 (FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G014500 PE=3 SV=1)

HSP 1 Score: 213.0 bits (541), Expect = 7.0e-52
Identity = 98/116 (84.48%), Postives = 107/116 (92.24%), Query Frame = 0


Query: 9   KGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDTE 68
           KGLRFGVMPC++NTVYWF TWRPSKQ EE EENP++LK+H+L KLGKVPDQ RAVVEDTE
Sbjct: 206 KGLRFGVMPCNSNTVYWFATWRPSKQEEEIEENPTKLKEHILRKLGKVPDQARAVVEDTE 265

Query: 69  VDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           VDSFVSLPLRYRHPW+LV NNFSR N+TIAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 266 VDSFVSLPLRYRHPWELVCNNFSRSNITIAGDALHSMTPDLGQGGCSALEDGVILA 321

[Close]

BLAST of Moc04g04700 vs. ExPASy TrEMBL
Match: A0A5A7U5X4 (FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold76G001200 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-50
Identity = 97/117 (82.91%), Postives = 108/117 (92.31%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC++NTVYWF TW PSKQ EE EENP++LK+H+LSKLGKVPDQ RAVVEDT
Sbjct: 204 SKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAVVEDT 263

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VD+FVSLPLRYR PW+LV NNFSRGNV+IAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 264 QVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVILA 320

[Close]

BLAST of Moc04g04700 vs. ExPASy TrEMBL
Match: A0A5D3CL17 (FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00440 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-50
Identity = 97/117 (82.91%), Postives = 108/117 (92.31%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC++NTVYWF TW PSKQ EE EENP++LK+H+LSKLGKVPDQ RAVVEDT
Sbjct: 184 SKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAVVEDT 243

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VD+FVSLPLRYR PW+LV NNFSRGNV+IAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 244 QVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVILA 300

[Close]

BLAST of Moc04g04700 vs. ExPASy TrEMBL
Match: A0A1S3BIK9 (FAD-dependent urate hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103490274 PE=3 SV=1)

HSP 1 Score: 209.1 bits (531), Expect = 1.0e-50
Identity = 97/117 (82.91%), Postives = 108/117 (92.31%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC++NTVYWF TW PSKQ EE EENP++LK+H+LSKLGKVPDQ RAVVEDT
Sbjct: 204 SKGLRFGVMPCNSNTVYWFATWCPSKQEEEIEENPTKLKEHILSKLGKVPDQARAVVEDT 263

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VD+FVSLPLRYR PW+LV NNFSRGNV+IAGDALH MTPDL QGGCSALEDGV+LA
Sbjct: 264 QVDAFVSLPLRYRRPWELVCNNFSRGNVSIAGDALHSMTPDLGQGGCSALEDGVILA 320

[Close]

BLAST of Moc04g04700 vs. ExPASy TrEMBL
Match: A0A6J1HW45 (monooxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111468075 PE=3 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 2.5e-49
Identity = 97/117 (82.91%), Postives = 106/117 (90.60%), Query Frame = 0


Query: 8   SKGLRFGVMPCSANTVYWFMTWRPSKQ-EETEENPSRLKQHLLSKLGKVPDQIRAVVEDT 67
           SKGLRFGVMPC+ANTVYWF TW  SKQ +E EENP RLKQH+LSKLGKVPD++RAVVED+
Sbjct: 225 SKGLRFGVMPCNANTVYWFSTWHSSKQGKEIEENPVRLKQHILSKLGKVPDRVRAVVEDS 284

Query: 68  EVDSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           +VDSFVSLPLR RHPW+LV N+FSRGNV IAGDALH MTPDL QGGCSALEDGVVLA
Sbjct: 285 KVDSFVSLPLRCRHPWELVWNDFSRGNVCIAGDALHSMTPDLGQGGCSALEDGVVLA 341

[Close]

BLAST of Moc04g04700 vs. TAIR 10
Match: AT4G38540.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 133.7 bits (335), Expect = 1.0e-31
Identity = 60/114 (52.63%), Postives = 83/114 (72.81%), Query Frame = 0


Query: 10  GLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVD 69
           G+R G +PC  NTVYWF+T   S   + E N   LK+ +L+K+  +P+ I+ VVE T++D
Sbjct: 203 GVRSGFIPCDHNTVYWFLT-HTSTDIDEETNSEILKEFVLNKIKDLPENIKNVVETTDLD 262

Query: 70  SFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           S V   L+YR PW+L+ +N ++ NV +AGDALHPMTPD+ QGGCSA+EDGV+LA
Sbjct: 263 SMVMSQLKYRPPWELLWSNITKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILA 315

[Close]

BLAST of Moc04g04700 vs. TAIR 10
Match: AT5G05320.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 131.7 bits (330), Expect = 4.0e-31
Identity = 57/114 (50.00%), Postives = 84/114 (73.68%), Query Frame = 0


Query: 10  GLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEVD 69
           G+R G + C  NTVYWF+T   +  +  ++N  ++KQ +L+K+  +PD I++++E T++D
Sbjct: 203 GVRSGFISCDQNTVYWFLT--HTSTDLDKKNHQKIKQFVLTKIKDLPDNIKSILETTDLD 262

Query: 70  SFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           S V  PL YR PW+L+  N ++ NV +AGDALHPMTPD+ QGGCSA+EDGV+LA
Sbjct: 263 SLVMNPLMYRPPWELLWANIAKDNVCVAGDALHPMTPDIGQGGCSAMEDGVILA 314

[Close]

BLAST of Moc04g04700 vs. TAIR 10
Match: AT2G29720.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 87.8 bits (216), Expect = 6.6e-18
Identity = 42/115 (36.52%), Postives = 65/115 (56.52%), Query Frame = 0


Query: 9   KGLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEV 68
           +GLR G +P SA  VYWF+T+          +P+ L++     +   P+ ++ +++ T  
Sbjct: 239 RGLRAGYVPVSATKVYWFITFNSPSLGPQMMDPAILRKEAKELVSTWPEDLQNLIDLTPD 298

Query: 69  DSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           ++    PL  R  W  +  + S+G V + GDA HPMTP+L QG C ALED V+LA
Sbjct: 299 EAISRTPLADRWLWPGIAPSASKGRVVLVGDAWHPMTPNLGQGACCALEDSVLLA 353

[Close]

BLAST of Moc04g04700 vs. TAIR 10
Match: AT2G35660.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 84.7 bits (208), Expect = 5.6e-17
Identity = 42/115 (36.52%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 9   KGLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEV 68
           KG+R G +P S   VYWF+ +          +P+ LK+     +   P+ ++ +++ T  
Sbjct: 241 KGIRAGYVPVSTTKVYWFICFNSPSLGPKITDPAILKKQAKELVSTWPEDLQNLIDLTPD 300

Query: 69  DSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           ++    PL  R  W  +    S+G V + GDA HPMTP+L QG C ALED VVLA
Sbjct: 301 ETISRTPLVDRWLWPGIAPPASKGRVVLVGDAWHPMTPNLGQGACCALEDSVVLA 355

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BLAST of Moc04g04700 vs. TAIR 10
Match: AT2G35660.2 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 84.7 bits (208), Expect = 5.6e-17
Identity = 42/115 (36.52%), Postives = 62/115 (53.91%), Query Frame = 0


Query: 9   KGLRFGVMPCSANTVYWFMTWRPSKQEETEENPSRLKQHLLSKLGKVPDQIRAVVEDTEV 68
           KG+R G +P S   VYWF+ +          +P+ LK+     +   P+ ++ +++ T  
Sbjct: 127 KGIRAGYVPVSTTKVYWFICFNSPSLGPKITDPAILKKQAKELVSTWPEDLQNLIDLTPD 186

Query: 69  DSFVSLPLRYRHPWQLVRNNFSRGNVTIAGDALHPMTPDLSQGGCSALEDGVVLA 124
           ++    PL  R  W  +    S+G V + GDA HPMTP+L QG C ALED VVLA
Sbjct: 187 ETISRTPLVDRWLWPGIAPPASKGRVVLVGDAWHPMTPNLGQGACCALEDSVVLA 241

The following BLAST results are available for this feature:

BLAST of Moc04g04700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144855.1	1.5e-51	84.48	monooxygenase 2 [Cucumis sativus] >KGN43264.1 hypothetical protein Csa_020371 [C...	[more]
XP_038887729.1	5.5e-51	83.76	monooxygenase 2-like [Benincasa hispida]	[more]
XP_008447940.1	2.1e-50	82.91	PREDICTED: FAD-dependent urate hydroxylase-like [Cucumis melo] >KAA0049767.1 FAD...	[more]
TYK12110.1	2.1e-50	82.91	FAD-dependent urate hydroxylase-like [Cucumis melo var. makuwa]	[more]
XP_022968891.1	5.2e-49	82.91	monooxygenase 2-like, partial [Cucurbita maxima]	[more]

BLAST of Moc04g04700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81816	1.5e-30	52.63	Monooxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MO2 PE=2 SV=1	[more]
Q9FLC2	5.6e-30	50.00	Monooxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=MO3 PE=2 SV=1	[more]
O81815	1.4e-12	35.45	Monooxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MO1 PE=2 SV=1	[more]
H1ZZA4	1.5e-11	34.21	Aurachin C monooxygenase/isomerase OS=Stigmatella aurantiaca OX=41 GN=auaG PE=1 ...	[more]
Q6F6Y2	1.3e-07	28.70	FAD-dependent urate hydroxylase OS=Acinetobacter baylyi (strain ATCC 33305 / BD4...	[more]

BLAST of Moc04g04700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0K2K7	7.0e-52	84.48	FAD_binding_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G0145...	[more]
A0A5A7U5X4	1.0e-50	82.91	FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A5D3CL17	1.0e-50	82.91	FAD-dependent urate hydroxylase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E...	[more]
A0A1S3BIK9	1.0e-50	82.91	FAD-dependent urate hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103490274 PE=...	[more]
A0A6J1HW45	2.5e-49	82.91	monooxygenase 2-like OS=Cucurbita maxima OX=3661 GN=LOC111468075 PE=3 SV=1	[more]

BLAST of Moc04g04700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38540.1	1.0e-31	52.63	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT5G05320.1	4.0e-31	50.00	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G29720.1	6.6e-18	36.52	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G35660.1	5.6e-17	36.52	FAD/NAD(P)-binding oxidoreductase family protein	[more]
AT2G35660.2	5.6e-17	36.52	FAD/NAD(P)-binding oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002938	FAD-binding domain	PFAM	PF01494	FAD_binding_3	coord: 7..123 e-value: 6.8E-8 score: 32.1
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 1..123 e-value: 1.9E-20 score: 75.4
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 78..123
None	No IPR available	PANTHER	PTHR45934:SF20	MONOOXYGENASE 2-RELATED	coord: 8..123
IPR044560	Monooxygenase/2-heptyl-3-hydroxy-4(1H)-quinolone synthase	PANTHER	PTHR45934	FAD/NAD(P)-BINDING OXIDOREDUCTASE FAMILY PROTEIN	coord: 8..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g04700.1	Moc04g04700.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0050790	regulation of catalytic activity
biological_process	GO:0042176	regulation of protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000502	proteasome complex
molecular_function	GO:0030234	enzyme regulator activity
molecular_function	GO:0071949	FAD binding
molecular_function	GO:0004497	monooxygenase activity