Moc04g04440 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g04440
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	O-methyltransferase family protein
Location	chr4: 2864130 .. 2870570 (+)
RNA-Seq Expression	Moc04g04440
Synteny	Moc04g04440

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAAGAATGCACCGGACCAGCACCAAGCGCTGCGAAAAGGGAGAGTTGAGTGCATCAGAAGAGGAAGGCCGAGTGCACCATACGGACGTCCCCAATACAGTTAAAGGTGCCAACAAACTCACCCAACGGGCATCTGGCACGGTGTCAGAAGAGTACTCTAGGCACCGCACAGGAATAGGACACGGAATGTCAAGAGAGGTCCGAACACCCTCGATTTGTTATCCATCATGCTCTACCTCTCTCATCCACTCTCCATATGTAAGGAAAAGAGGAATCCTAGATTTTTCTTACACACAACTCTTTATGAGAATAATTCGACACACAATTGGGAGTAGACTCTCACTCTCGAAATTCCCTTCTACTCTATTTGCTTCATACCTATTTCCTCTCCATTTGAATTTTAATTTGAGTATCAAAGTGCATTCGACATAGTACTACATTAGTACTAATTTTCCTGGAGGTGTTAAGAGTTTGGACACTTACGTGACACCCATGAAGTGAAATTGCATTAACAAGTACAAAGTTGAAAAATAATAAGGAACAAAATGAAAAGAAATAAATAAAAAAAAACACATCACAAAACAAATAATTGAGATTTCATTAAGATTCTCCACTTCTTATTGTCTAGAAAAGACAAAAGAAAAAGGAAGGTTACAAAATAATTAATTTCAATCCAGCAATGATGACCTTCTACAAGGTCTAATTTTTAAACCGAAGTTTCCCACCATAGATGGCCAATTTTATTTAATTATAAAGCTCAATGATTCTTTTTCTCGTATGTTTAATAATGTAAAAGAAACGGTTCAACTAATTTACTACGGTCGGATGCTATGTTGTGGGGTTACAAAATAATTGAAAATCACTTTTTTTTCATGTTAAATTACAAAGTGTAGAGATCGAATCAACAATCTCTTGATTGAAAATATAAATCAATTACCGCTGAGTTATATTTATTTTTATCTATTAATGACAATATATCCTATCTAAATATCTCTACCATCTGATATTGGTTCGCTTTGATCATAAATCTTTACTTTTGGTTTCACCCAATCTATCGACCTTTCTCGAATCTATGTGAGATTTTGTAATACCAACATATTTTATGTGAGATGTTGATTATTTAAAATTATGGCAAAAATAAATAAATTTTTAAAGAAAATTTTAAAAATGAGTGATTTCTTGGTTTTTGATTTTCTAATTTTAAAAATTAAGTTGGGAAACACTATGTTTCTTTGTCCAGCTATATACTCTTTAAAAATGTTGTCAAAATCTAAGCAGAATTTTAAAAACCTAAAAGAATATAGTTTCTGACAAATTATTTTTTATCTTTAAATGTAACAACGAATTAATACATGATGTAAAAAACATATCTAAGAAAGAATATAAATCCAGTATAATTTTTAAAAATAGAATAATTAACGAATGGATTTAAACGAATTTAATTATGATAATATTATTTGGACGCGCCTTTTATCAAATACTAGTAAGAAAATAAGACAAGGATTGAATAGAATATAGAGTAGGTAACGTTATTTTTTTTAAGAAAAACAAAATATGACATCAAACTTCTAGCTCAATTGGGTTTAAAAAAAGACTGTAAAAAAACATTTAGTAAAATTAGTAAAAATAGTAACTATGTTTATTTACTTTTATGATGGCATAGTTCTCACGGCTTTGCTTTTGGTTTCACCCAAAAAATCTCAGTCCAATGAAGATAATTGTCCTCTTTTATATACCCATGATCATTCCCTTTCATATCGAATGTGGAACTTTGTTTGCAATCACCTTACAAACCCAACACGACTCCGACGAGTGGTGGCTTGATCTGTCGAACGCGACCTAAGTCATATTGTTGCTTATGAAGACATTGAAAACGGTCTTCTTCTCGTTGGCGACAACCCAAATCACAAAAAATAATAATAATGAAAATAAGAATTCTGTTGAGAGTGACAGAGGAGGGAGAAGCGTGGAGAAGAGGGAAATGAAGGAAAAAACAACAAATAAGTTAAACAAATAATATATATATAATAAAATATAAAACTCACTGACGCCATGTCAGCAATCTGTTACCACAGTTGTTTTGATTTTAGGGATATTTTAGTCTGCGTTAAATTTTCACTTTTTGAAATTTAGTAACCAAATAAAAACCTAGCTTCAAACTCATTGATTAAAAATGTATTTTACCTAGCTTTTTCAAATATAGTACTTTAAAAAAAATGGAGACTCAATTTTAAAATGACAACTTTACTCAAATGATATACCTCTGTAATATATGTGTGTGATATACATATGATATACTACTGATATGAGTGTCTCATATACCTACAATATGTATATGATATACAACTGATATAGGATGTGCATATAATATACTATAATACTGATATAAGTAAAAAATTTTACTAGATTTAAAAATTTTTAAATAGGTGACACATTTTGAAATAAATAAAAGATTTTTACTGCAAACCGCAATTTCACTAAAAACCATATTAATTTTGAAGACCAAAAACTTATATATATAAAATTCTTTTTAGGGACAACACATTATGCTAAATATTGAAAGTTAGTTTTTTTTTTATTTTTTCCCCTTTTTTTTTGCATCAACATCTTCTTCTTCTTCTTTTTTTTTTTTCCTTTTTTGTTCGTTTTTGAGAAAAGAATATTCTAATCAAATGAGTACAAGTACAGCTAATTCCAACTCATGTAATGAGTTATTTTGTACTGCTAAAATAATTATGCTGAAATTACAATTTACAAGCTTTCTATGGAGAAATTTCCTACGATTGAGTTGTTTCATCACTCTATTCAACCAATTAACGAAGTTTGGAGGAAGAAATGTGTATAAATACTGATCATTGTTAGCTTTTTAGATTATAATCTAAACCAATTATTCAATTTTAATGAGAACTTACTAAAATTTTCCACCTTTCAAACTCTGCTAGTTTCTCAATATTTCTTGGTTCCTCAAATTTAAATTAACTCTACTTGTTTTATTACTTTTATTTCATTTTCATATTTACCAACTTGTTATATGTATTAACTTACCTTACTTTTATTTATGTTTTAAAAAACAATGAAAACATGGAATTTCATTAAAAGTTGTTTGGCGAGAATTTTTTTAAATATGTTGATATATTTTAATTAGTTAATATATAGGGATATATTCTTATTTATTTATTTAGATGGATATATTATAATTATACATAAAGATTCGAACTATCAACTTAGCATTAAATCAGATTTAAATTGTAATGGCTTTGAAGTTTTTCAGTATAACTTTAAATTTTTTAAAATATGTTGGTATATTTTAATTAGTTAATATATAAGTATATATTCTTATTTATTTATTCATATTTTGTTTATTTAGATGGATATATTATAATTATACCTAAAGATTCATACCGAAAAGATTTTTCTGTGATTAACTATTAAATCATATTTAAATTGTAGTGTATGACATGAATTTATATAGATATTTAGATGAATATATTACAATATAGGGTAAAGATTCGAATTTTAGACTTAGAATAATTTTTTAAAGTTGTGAATCCACGTCAAACATGAATTCATGTAATATCTATTAAATATTTGTCTTCTTTCAATTATTTTATTTAGGTTAAAAAACAAATTTTGATCCTGAAAGTAACGATAAACTTTAGTTTGTCGCAATTTAGTCCTTATAATTTCACTAGAATAATGATTTCGTCACTAAACTTTAGTTCGTAACATATTATTGTACATTAAAACCAAAAGTATTTTTTAACCTTCTATATATCAATAGACTTTTGTATATTTTATGTAGCAAGACTATATTTAGTCTATAATTATTATTTTTATAGTGATATTCTATTTAATGTACAATATTATATTATGAATTCATTCTATTTTTTATAAAAATAAAAATATTATATTATATTATAAATTGTATAAGATTTTACTTTGTAACCTATTTTTAGTTTTTGTTACCTATTTTATCATTGATTGAACCAAACATAGTTTCAATAAATTCTTAATTTTATTTCTAATTTTTCAATCTACTACCAAACATATGTGAATATGTGAATAGATAGAATACCACTCTTATTTTCATTGAATCCATTATTTCAAGAATCATGCTTTCATATCACCGATCAAAAGCACCATTCACTAAACTAGTCAATAATAACATTTTTAGATATTGGATTTGAATAAAACTATCTCTCAATAAGTACTCATGTCACTATTCAATTTTTTACAGCACATCATTTTTTAAAAAATATATTCTATTTTCATATTTTTCTCTTATTTATCAAAATTTTAACATCGATTAGCACAAAATAAATGCTTACGAGTAAATATCTAATTATTTTCTTTAATTAATAAAAAAATTTAGTCGGTTTAACCTTTTCTTTTTGGATTCAAATTTCTAATCTTAAATTATACATTTCCTCAAAACAATGTTAAATAAAGCTTGGTGCCATCTTTGATAGTACTAAAATATTATCATCTATATCAAACCACTGTCTCCAATAATGGCAATGAGCAATTTTCCCTTTCCTATATAATTAAGAACTTGCAAGAACAACAAAGATAATCACTGAAGCTAGTTAGATCTCATAGTTATATCGTGATGGAAATTTCTTGTTCCAAAAAGGAACAAGAAGATCATAGCGAAGCTCGAACCCAAGTATGGAAATACATATTTGGTTTTGTCGAAATGGCGGTAATAAAATGTGCAATCGAGCTCAAGATCGACGACGCCATTGAAAGTCATGGAAGCCCCATAACACTTCCACAGCTATCATCAGCTCTAAAATGCTCTCCACCAAGCTTACACCGCGTGTTGCGGTTCCTCGTTCACCGAGGCATTTTCAAAGAAGAAGAAACTGCAATCGAAGGCGTGAAAGGCTACACTCAAACGCCAATGTCTAGGCTGCTCACTAGCAGCAGCAAAAGCAGCATGGCTCCTCTGCTGCTGCTAGAGAGCAACCCGGTAATGCTCGCGCCATGGCATAAGCTAAGTGCATTTCTGAAGGACGACGACACTCCGCCATTCGAGGCTGCCCACGGGAGGGATTTGTGGAGCTATGCTGCAGCGGATCCGTCGTTCACCATGCTGCTCAATGAGGCCATGGCTTGTTTTGCGAGGCTGGTGATTGTGCCTGCAGTGATTGAAAGGTGTGGGGAGATTTTTGAAGGGGTTGGATCCTTGGTGGACGTGGGGGGAGGAAATGGGACTTGTTTGAGCATCTTGGTTAAGGCTTTTCCTTGGATTAGAGGCATTAATTTTGACCTTCCACATGTCGTGTTGGTCTCACCAAACTATGAGGGCGTCAAACATGTTGGTGGCAACATGTTTGATTGTGTCCCTAAGGCTGATGCTGCTTTCATCATGGTAAATTTAGAACTCGTTTATAACGTCTTTATTACAAAAATAAATTAGAATGGTATGTTCGATAATTGTTTTTTTGTTTCTTATTATTTTAGCATAAATATTTGTTTTGTAACTTTTTTTTTTGTTTTGCTTCGTGTTCTCTAAAATTTTAAATTCGAAAAAGAAAAAGTTGTATATAAAAGTTAAAATTTTAAATTAAATATTCAAAAGTTTTATGTTATTATTTAATTTTTTATATTTATTATGCATTTTATAAATAAAAAATGATAAAATAATACTTTTTGGAAAATACTTAGTTCTAACTGGTGATGCCCTATCTTTTATCACAACCATTAAATGTTTGACAATATTGGTAAAAATAAAGAATTTTTCTAAAAAATGATGAGTTGTCAAACTTTCAACCTTAAATACTTTTCATGCTTTTATTTATGTTTTTGGTGAAAAACTTGAACAAAAACTTGATTTTGTTGTTTCTAAAATTTATACACAATTTTGAAAACGTTTCTTAAAAGCGAGAAACGATCAAGAAACATGAACCGCTTACCAAACGCGCACTTATGTTTTTCAAAAATTGAAAATGAAAACAAGAAACAGTAACATTACCACTTTTAGGATTAAAAACTAAAAATCTAGTTCATGGTGTTATTTAATTTTACTGTTATTTTCTATCATTCTGCCCTCATTGATGTTAATATCTCGTTCATATACATATATATATATATGCTTCCAATAATGTACAATATAAATGACTTTAAGGTGTTATGCAGGAGGTTTTACATGATTGGGACGATGAAGAATGCATCAAACTTTTGAAAAACTGCAAAGAAGCAATTCCAAAAAAGACTGGAAAAGTTATAATCGTTGAAATCGTGATCGATGAAAAGGAACAAAATAAATTGTTAGACGTAAGGTTGATGATGGATATGATAATGATGGCGCATACCAATAAAGGCAAGGAAAGAACACGTGAGGAATGGGCCTATGTTCTTCAAAAGGCTGGTTTTAGTCGATATATCATCACTCCCACTTCAACCATCCAATCTGTTATTCAAGCTTTTCCTTAA

mRNA sequence

ATGGAAAGAATGCACCGGACCAGCACCAAGCGCTGCGAAAAGGGAGAGTTGAGTGCATCAGAAGAGGAAGGCCGAGTGCACCATACGGACGTCCCCAATACAGTTAAAGGTGCCAACAAACTCACCCAACGGGCATCTGGCACGGTGTCAGAAGAGTACTCTAGGCACCGCACAGGAATAGGACACGGAATGTCAAGAGAGGAACAAGAAGATCATAGCGAAGCTCGAACCCAAGTATGGAAATACATATTTGGTTTTGTCGAAATGGCGGTAATAAAATGTGCAATCGAGCTCAAGATCGACGACGCCATTGAAAGTCATGGAAGCCCCATAACACTTCCACAGCTATCATCAGCTCTAAAATGCTCTCCACCAAGCTTACACCGCGTGTTGCGGTTCCTCGTTCACCGAGGCATTTTCAAAGAAGAAGAAACTGCAATCGAAGGCGTGAAAGGCTACACTCAAACGCCAATGTCTAGGCTGCTCACTAGCAGCAGCAAAAGCAGCATGGCTCCTCTGCTGCTGCTAGAGAGCAACCCGGTAATGCTCGCGCCATGGCATAAGCTAAGTGCATTTCTGAAGGACGACGACACTCCGCCATTCGAGGCTGCCCACGGGAGGGATTTGTGGAGCTATGCTGCAGCGGATCCGTCGTTCACCATGCTGCTCAATGAGGCCATGGCTTGTTTTGCGAGGCTGGTGATTGTGCCTGCAGTGATTGAAAGGTGTGGGGAGATTTTTGAAGGGGTTGGATCCTTGGTGGACGTGGGGGGAGGAAATGGGACTTGTTTGAGCATCTTGGTTAAGGCTTTTCCTTGGATTAGAGGCATTAATTTTGACCTTCCACATGTCGTGTTGGTCTCACCAAACTATGAGGGCGTCAAACATGTTGGTGGCAACATGTTTGATTGTGTCCCTAAGGCTGATGCTGCTTTCATCATGGAGGTTTTACATGATTGGGACGATGAAGAATGCATCAAACTTTTGAAAAACTGCAAAGAAGCAATTCCAAAAAAGACTGGAAAAGTTATAATCGTTGAAATCGTGATCGATGAAAAGGAACAAAATAAATTGTTAGACGTAAGGTTGATGATGGATATGATAATGATGGCGCATACCAATAAAGGCAAGGAAAGAACACGTGAGGAATGGGCCTATGTTCTTCAAAAGGCTGGTTTTAGTCGATATATCATCACTCCCACTTCAACCATCCAATCTGTTATTCAAGCTTTTCCTTAA

Coding sequence (CDS)

ATGGAAAGAATGCACCGGACCAGCACCAAGCGCTGCGAAAAGGGAGAGTTGAGTGCATCAGAAGAGGAAGGCCGAGTGCACCATACGGACGTCCCCAATACAGTTAAAGGTGCCAACAAACTCACCCAACGGGCATCTGGCACGGTGTCAGAAGAGTACTCTAGGCACCGCACAGGAATAGGACACGGAATGTCAAGAGAGGAACAAGAAGATCATAGCGAAGCTCGAACCCAAGTATGGAAATACATATTTGGTTTTGTCGAAATGGCGGTAATAAAATGTGCAATCGAGCTCAAGATCGACGACGCCATTGAAAGTCATGGAAGCCCCATAACACTTCCACAGCTATCATCAGCTCTAAAATGCTCTCCACCAAGCTTACACCGCGTGTTGCGGTTCCTCGTTCACCGAGGCATTTTCAAAGAAGAAGAAACTGCAATCGAAGGCGTGAAAGGCTACACTCAAACGCCAATGTCTAGGCTGCTCACTAGCAGCAGCAAAAGCAGCATGGCTCCTCTGCTGCTGCTAGAGAGCAACCCGGTAATGCTCGCGCCATGGCATAAGCTAAGTGCATTTCTGAAGGACGACGACACTCCGCCATTCGAGGCTGCCCACGGGAGGGATTTGTGGAGCTATGCTGCAGCGGATCCGTCGTTCACCATGCTGCTCAATGAGGCCATGGCTTGTTTTGCGAGGCTGGTGATTGTGCCTGCAGTGATTGAAAGGTGTGGGGAGATTTTTGAAGGGGTTGGATCCTTGGTGGACGTGGGGGGAGGAAATGGGACTTGTTTGAGCATCTTGGTTAAGGCTTTTCCTTGGATTAGAGGCATTAATTTTGACCTTCCACATGTCGTGTTGGTCTCACCAAACTATGAGGGCGTCAAACATGTTGGTGGCAACATGTTTGATTGTGTCCCTAAGGCTGATGCTGCTTTCATCATGGAGGTTTTACATGATTGGGACGATGAAGAATGCATCAAACTTTTGAAAAACTGCAAAGAAGCAATTCCAAAAAAGACTGGAAAAGTTATAATCGTTGAAATCGTGATCGATGAAAAGGAACAAAATAAATTGTTAGACGTAAGGTTGATGATGGATATGATAATGATGGCGCATACCAATAAAGGCAAGGAAAGAACACGTGAGGAATGGGCCTATGTTCTTCAAAAGGCTGGTTTTAGTCGATATATCATCACTCCCACTTCAACCATCCAATCTGTTATTCAAGCTTTTCCTTAA

Protein sequence

MERMHRTSTKRCEKGELSASEEEGRVHHTDVPNTVKGANKLTQRASGTVSEEYSRHRTGIGHGMSREEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP

Homology

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BLAST of Moc04g04440 vs. NCBI nr
Match: XP_022135686.1 ((R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia])

HSP 1 Score: 696.8 bits (1797), Expect = 1.1e-196
Identity = 346/348 (99.43%), Postives = 348/348 (100.00%), Query Frame = 0


Query: 65  SREEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP 124
           S++EQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP
Sbjct: 6   SKKEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP 65

Query: 125 PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA 184
           PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA
Sbjct: 66  PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA 125

Query: 185 PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 244
           PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG
Sbjct: 126 PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 185

Query: 245 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 304
           EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC
Sbjct: 186 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 245

Query: 305 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM 364
           VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM
Sbjct: 246 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM 305

Query: 365 MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP
Sbjct: 306 MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 353

[Close]

BLAST of Moc04g04440 vs. NCBI nr
Match: XP_004144871.1 (acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical protein Csa_020497 [Cucumis sativus])

HSP 1 Score: 531.6 bits (1368), Expect = 6.2e-147
Identity = 267/349 (76.50%), Postives = 300/349 (85.96%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLH 128
           +E+ S+ + Q+WKYIFGFVEMAVIKCAIELKI D IESHG  +TL QLSS+L CSP  LH
Sbjct: 15  EEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLH 74

Query: 129 RVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHK 188
           R+LRFLVHRGIFKEE+T     K Y+ TPMSRLLT++  +SMAPL LLES+PVM+APWH 
Sbjct: 75  RILRFLVHRGIFKEEKT----TKCYSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHN 134

Query: 189 LSAFLKDDD----TPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
           LSA +K  D    + PFE AHG DLW+YAAA+PS + L+NE+MACFARLVI+PAV+ERCG
Sbjct: 135 LSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLERCG 194

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           EIF+GVG LVDVGGGNGTCLSILVKA PWI+GINFDLPHVV VS  YEG++HVGGNMFD 
Sbjct: 195 EIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMFDS 254

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQ-NKLLDVRL 368
           VPKADAAFIMEVLHDWDDEECIK+LKNCKEAIP+KTGKVIIVE+VIDEKE+ +K  D RL
Sbjct: 255 VPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDARL 314

Query: 369 MMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           MMDMIMMAHT KGKERT EEWA VLQKAGFS Y ITPTSTIQSVIQAFP
Sbjct: 315 MMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

[Close]

BLAST of Moc04g04440 vs. NCBI nr
Match: KAA0040348.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23356.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 522.7 bits (1345), Expect = 2.9e-144
Identity = 262/350 (74.86%), Postives = 300/350 (85.71%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLH 128
           +E++S+ + QVWKYIFGFVEMAV+KCAIELKI D IESHG  ITL QLSS+L CSP  LH
Sbjct: 15  EEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLSQLSSSLSCSPLLLH 74

Query: 129 RVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHK 188
           R+LRFLVHRGIFKEE+++    K Y+ TPMSRLLT++  +SMAP+ LLES+PVM+APWH 
Sbjct: 75  RILRFLVHRGIFKEEKSS----KSYSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHN 134

Query: 189 LSAFLK----DDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
           LSA +K    ++ + PFE AHG DLW+YAAA+PS + LLNE MACFARL I+PAV+ERCG
Sbjct: 135 LSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLFILPAVLERCG 194

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           EIF+GVG LVDVGGGNGTCLSILVKA PWI+GINFDLPHVV VS  YEGV+HVGG+MFD 
Sbjct: 195 EIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGVQHVGGDMFDS 254

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQ--NKLLDVR 368
           VPKADAAFIMEVLHDWDDEECIK+L+NCKEAIP+KTGKVIIVEIVIDEKE   +K  DV+
Sbjct: 255 VPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKEDQISKYSDVK 314

Query: 369 LMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           L MDMIMMAHT KGKERT EEWA +LQKAGFS Y ITPT+TIQSVIQAFP
Sbjct: 315 LRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

[Close]

BLAST of Moc04g04440 vs. NCBI nr
Match: XP_008447905.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 521.5 bits (1342), Expect = 6.4e-144
Identity = 261/350 (74.57%), Postives = 300/350 (85.71%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLH 128
           +E++S+ + QVWKYIFGFVEMAV+KCAIELKI D IESHG  +TL QLSS+L CSP  LH
Sbjct: 15  EEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQLSSSLSCSPLLLH 74

Query: 129 RVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHK 188
           R+LRFLVHRGIFKEE+++    K Y+ TPMSRLLT++  +SMAP+ LLES+PVM+APWH 
Sbjct: 75  RILRFLVHRGIFKEEKSS----KSYSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHN 134

Query: 189 LSAFLK----DDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
           LSA +K    ++ + PFE AHG DLW+YAAA+PS + LLNE MACFARL I+PAV+ERCG
Sbjct: 135 LSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLFILPAVLERCG 194

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           EIF+GVG LVDVGGGNGTCLSILVKA PWI+GINFDLPHVV VS  YEGV+HVGG+MFD 
Sbjct: 195 EIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGVQHVGGDMFDS 254

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQ--NKLLDVR 368
           VPKADAAFIMEVLHDWDDEECIK+L+NCKEAIP+KTGKVIIVEIVIDEKE   +K  DV+
Sbjct: 255 VPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKEDQISKYSDVK 314

Query: 369 LMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           L MDMIMMAHT KGKERT EEWA +LQKAGFS Y ITPT+TIQSVIQAFP
Sbjct: 315 LRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

[Close]

BLAST of Moc04g04440 vs. NCBI nr
Match: KAG6572137.1 (Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 520.8 bits (1340), Expect = 1.1e-143
Identity = 255/346 (73.70%), Postives = 297/346 (85.84%), Query Frame = 0


Query: 68  EQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSL 127
           E+E++++ + QVWKYIFGFVEMAVIKCAIELKI D IE+HG  +TL +LSS+L CSP  L
Sbjct: 14  EEEENNDIKIQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTELSSSLNCSPLLL 73

Query: 128 HRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWH 187
           HR+LRFL+HRGIFKEE T     K Y QTPMSRLLTSS   S+AP+ +LES+ VMLAPWH
Sbjct: 74  HRILRFLIHRGIFKEEPT-----KSYAQTPMSRLLTSSGAHSLAPMHVLESSHVMLAPWH 133

Query: 188 KLSAFLKDDD--TPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCGE 247
            LSA+LK +D  + PFE AHG+DLW Y AA+ S   ++NE MA FAR+VIVPAV+ERCG 
Sbjct: 134 NLSAYLKANDGGSQPFEMAHGKDLWRYCAANSSHNKVMNEGMASFARVVIVPAVLERCGG 193

Query: 248 IFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDCV 307
           +FEGVGSLVDVGGGNG+CLS+LVKAFPWIRGINFDLPHVV VS  YEGV+HVGGNMFD V
Sbjct: 194 VFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEGVRHVGGNMFDFV 253

Query: 308 PKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLMM 367
           PKADAAFIM  LHDWDDE+CIK+LKNCKEAIP+KTGKVIIVE+VIDEK+++ + D+RLM+
Sbjct: 254 PKADAAFIMNALHDWDDEDCIKILKNCKEAIPEKTGKVIIVEVVIDEKDESMVSDIRLML 313

Query: 368 DMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAF 412
           DMIMMAHT KGKERTREEWAYVLQ+AGFSR+ ITPTSTIQS+IQ F
Sbjct: 314 DMIMMAHTIKGKERTREEWAYVLQEAGFSRHTITPTSTIQSIIQGF 354

[Close]

BLAST of Moc04g04440 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 358.6 bits (919), Expect = 9.4e-98
Identity = 181/362 (50.00%), Postives = 251/362 (69.34%), Query Frame = 0


Query: 66  REEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGS--PITLPQLSSALKCS 125
           R+  ++ ++A   +WKY+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LSSA+  S
Sbjct: 20  RKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSAS 79

Query: 126 PPSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSR--LLTSSSKSSMAPLLLLESNPV 185
           P  L R++RFLVH+GIFKE  T      GY  TP+SR  ++T     S+AP +L E+ P 
Sbjct: 80  PSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPE 139

Query: 186 MLAPWHKLSAFLKD----DDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVP 245
           MLAPW +LS+ +         PPF+A HG+D+WS+A  +P  + ++NEAMAC AR V VP
Sbjct: 140 MLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VP 199

Query: 246 AVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHV 305
            V   C  +F+GV ++VDVGGG G  + +LVK FPWI+G NFDLPHV+ V+   +GV++V
Sbjct: 200 RVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENV 259

Query: 306 GGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNK 365
            G+MFD +P  DA FI  VLHDW D++CIK+LKNCKEA+P   GKV+IVE VI E ++  
Sbjct: 260 EGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTM 319

Query: 366 LLD--------VRLMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQ 412
           ++D        VRLM+DM+MMAHT+ GKERT +EW +VL++AGF+RY +     +QS+I 
Sbjct: 320 IVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLII 379

[Close]

BLAST of Moc04g04440 vs. ExPASy Swiss-Prot
Match: Q6WUC2 ((R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=1 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 9.1e-77
Identity = 155/353 (43.91%), Postives = 219/353 (62.04%), Query Frame = 0


Query: 70  EDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPS--- 129
           E+  + + ++W+++F FV+   +KCA+EL I D I SHG P+T+ ++  +LK + PS   
Sbjct: 5   EERLKGQAEIWEHMFAFVDSMALKCAVELGIPDIINSHGRPVTISEIVDSLKTNTPSSSP 64

Query: 130 ----LHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVM 189
               L R++R LVH+ +F  E         Y  T  S+ L   SK +++PL+L E+NP++
Sbjct: 65  NIDYLTRIMRLLVHKRLFTSELHQESNQLLYNLTRSSKWLLKDSKFNLSPLVLWETNPIL 124

Query: 190 LAPWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIER 249
           L PW  L    ++  + PFE AHG ++W  A ADP F   LN AM C    +I   ++E 
Sbjct: 125 LKPWQYLGKCAQEKSS-PFERAHGCEIWDLALADPKFNNFLNGAMQCSTTTIINEMLLE- 184

Query: 250 CGEIFEGV-GSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNM 309
             + F G+ GSLVDVGGG G+ ++ +VKA P I+GINFDLPHVV  +  + GVKHVGG+M
Sbjct: 185 YKDGFSGIAGSLVDVGGGTGSIIAEIVKAHPHIQGINFDLPHVVATAAEFPGVKHVGGDM 244

Query: 310 FDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAI-PKKTGKVIIVEIVIDEKEQNKLLD 369
           F  +P+ADA  +  +LHDW DE+C  +LKNC  AI  KK GKVIIV+ V+   + N L D
Sbjct: 245 FVDIPEADAVIMKWILHDWSDEDCTIILKNCYRAIRKKKNGKVIIVDCVL-RPDGNDLFD 304

Query: 370 -VRLMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
            + L+ D++MMAHT  GKERT  EW  +L  AGF RY +  T     +I+AFP
Sbjct: 305 KMGLIFDVLMMAHTTAGKERTEAEWKILLNNAGFPRYNVIRTPAFPCIIEAFP 354

[Close]

BLAST of Moc04g04440 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 6.1e-73
Identity = 145/349 (41.55%), Postives = 218/349 (62.46%), Query Frame = 0


Query: 67  EEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP-- 126
           E ++D+  ++ ++W +I+GF E  V+KCA++L + + I + G+ +TL +LSS L   P  
Sbjct: 2   EVKKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVN 61

Query: 127 -PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVML 186
             +L+RV+R+LVH  +F   + +I+G   Y   P ++ L       M   +L  ++   +
Sbjct: 62  EDALYRVMRYLVHMKLF--TKASIDGELRYGLAPPAKYLVKGWDKCMVGSILAITDKDFM 121

Query: 187 APWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERC 246
           APWH L   L  +    FE A G ++W Y A  P    L NEAMA  +RL I+ A+++ C
Sbjct: 122 APWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRL-IMSALVKEC 181

Query: 247 GEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFD 306
           G IF G+ +LVDVGGG GT +  +  AFP I+   +DLPHV+  SP Y  V  V G+MF 
Sbjct: 182 GNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTVYDLPHVIADSPGYSEVHCVAGDMFK 241

Query: 307 CVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRL 366
            +PKADA  +  +LHDWDD+ECI++LK CKEA+P K GKVIIV+IV++ + ++    +RL
Sbjct: 242 FIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMRL 301

Query: 367 MMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
            +D+ MM +T  GKERT EEW  ++  AG+  + IT  + +QSVI+A+P
Sbjct: 302 TLDLDMMLNTG-GKERTEEEWKKLIHDAGYKGHKITQITAVQSVIEAYP 346

[Close]

BLAST of Moc04g04440 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 270.0 bits (689), Expect = 4.4e-71
Identity = 144/348 (41.38%), Postives = 216/348 (62.07%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSAL---KCSPP 128
           QE   + + Q+W  I+GF +  V++CA+E+ I D I+++   ITL QL++ L     S  
Sbjct: 13  QEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAKLPITNVSSD 72

Query: 129 SLHRVLRFLVHRGIFKEEETAIEGV-KGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA 188
            L+R++R+LVH  I  E+ET   GV K Y+  P+  LL   ++ SM P++L  +    + 
Sbjct: 73  YLYRMVRYLVHLNII-EQETCNGGVEKVYSLKPVGTLLLRDAERSMVPMILGMTQKDFMV 132

Query: 189 PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
            WH +   L +  T  FE   G D+W Y   +P  + L NE MA   RL +   +IE C 
Sbjct: 133 SWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRL-LTKTLIEDCR 192

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           + F+G+ SLVD+GGGNGT +  + +AFP I+   +DLPHVV  S +   ++ V G+MF  
Sbjct: 193 DTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTLYDLPHVVANSHDLPNIEKVPGDMFKS 252

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM 368
           VP A A  +  +LHDW DEEC+ +LK CKEAIPK+TGKVIIV++ ++E+  ++L   RL+
Sbjct: 253 VPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEESNHELTKTRLI 312

Query: 369 MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           +D+ M+ +T  G+ERT ++W  +L++AGF  + I P   IQSVI+AFP
Sbjct: 313 LDIDMLVNTG-GRERTADDWENLLKRAGFRSHKIRPIRAIQSVIEAFP 357

[Close]

BLAST of Moc04g04440 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 7.5e-71
Identity = 138/348 (39.66%), Postives = 217/348 (62.36%), Query Frame = 0


Query: 70  EDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP---PS 129
           +++  ++ ++W +I+GF +  V+K A++L + + I +HGSP+TL +LS  L   P    +
Sbjct: 6   KENLSSQAKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELSLHLPSQPVNQDA 65

Query: 130 LHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPW 189
           L+RVLR+LVH  +F   +++I+G   Y   P ++ L       M   +L  ++   +APW
Sbjct: 66  LYRVLRYLVHMKLF--TKSSIDGELRYGLAPPAKFLVKGWDKCMLGAILTITDKDFMAPW 125

Query: 190 HKLSAFLKDD--DTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 249
           H L   + +D   +  FE A G ++W Y A  P    L NE MA   RL I+ A+++ C 
Sbjct: 126 HYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDTRL-IMSALVKECS 185

Query: 250 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 309
            +F+G+ ++VDVGGG GT +  + KAFP I+   +DLPHV+  SP Y  +  + G+MF  
Sbjct: 186 SMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTVYDLPHVIADSPGYTEINSIQGDMFKY 245

Query: 310 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM 369
           +P ADA  +  +LHDWDD+ECI++LK CK+A+P+  GKVII++I++D K ++    +RL 
Sbjct: 246 IPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDIILDVKSEHPYTKMRLT 305

Query: 370 MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           +D+ MM +T  GKERT EEW  ++  AG+  Y IT  S +QSVI+A+P
Sbjct: 306 LDLDMMLNTG-GKERTEEEWKKLIHDAGYKGYKITHISAVQSVIEAYP 349

[Close]

BLAST of Moc04g04440 vs. ExPASy TrEMBL
Match: A0A6J1C1G3 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007584 PE=3 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 5.4e-197
Identity = 346/348 (99.43%), Postives = 348/348 (100.00%), Query Frame = 0


Query: 65  SREEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP 124
           S++EQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP
Sbjct: 6   SKKEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSP 65

Query: 125 PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA 184
           PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA
Sbjct: 66  PSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLA 125

Query: 185 PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 244
           PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG
Sbjct: 126 PWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 185

Query: 245 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 304
           EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC
Sbjct: 186 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 245

Query: 305 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM 364
           VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM
Sbjct: 246 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM 305

Query: 365 MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP
Sbjct: 306 MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 353

[Close]

BLAST of Moc04g04440 vs. ExPASy TrEMBL
Match: A0A0A0K0Q2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 3.0e-147
Identity = 267/349 (76.50%), Postives = 300/349 (85.96%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLH 128
           +E+ S+ + Q+WKYIFGFVEMAVIKCAIELKI D IESHG  +TL QLSS+L CSP  LH
Sbjct: 15  EEEDSDIKVQIWKYIFGFVEMAVIKCAIELKIGDVIESHGDSMTLSQLSSSLNCSPLLLH 74

Query: 129 RVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHK 188
           R+LRFLVHRGIFKEE+T     K Y+ TPMSRLLT++  +SMAPL LLES+PVM+APWH 
Sbjct: 75  RILRFLVHRGIFKEEKT----TKCYSHTPMSRLLTTTGPNSMAPLHLLESSPVMIAPWHN 134

Query: 189 LSAFLKDDD----TPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
           LSA +K  D    + PFE AHG DLW+YAAA+PS + L+NE+MACFARLVI+PAV+ERCG
Sbjct: 135 LSACVKASDHENGSQPFEMAHGMDLWTYAAANPSHSSLINESMACFARLVILPAVLERCG 194

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           EIF+GVG LVDVGGGNGTCLSILVKA PWI+GINFDLPHVV VS  YEG++HVGGNMFD 
Sbjct: 195 EIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGIQHVGGNMFDS 254

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQ-NKLLDVRL 368
           VPKADAAFIMEVLHDWDDEECIK+LKNCKEAIP+KTGKVIIVE+VIDEKE+ +K  D RL
Sbjct: 255 VPKADAAFIMEVLHDWDDEECIKILKNCKEAIPEKTGKVIIVEVVIDEKEEISKYSDARL 314

Query: 369 MMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           MMDMIMMAHT KGKERT EEWA VLQKAGFS Y ITPTSTIQSVIQAFP
Sbjct: 315 MMDMIMMAHTIKGKERTNEEWANVLQKAGFSYYTITPTSTIQSVIQAFP 359

[Close]

BLAST of Moc04g04440 vs. ExPASy TrEMBL
Match: A0A5D3DI94 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004200 PE=3 SV=1)

HSP 1 Score: 522.7 bits (1345), Expect = 1.4e-144
Identity = 262/350 (74.86%), Postives = 300/350 (85.71%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLH 128
           +E++S+ + QVWKYIFGFVEMAV+KCAIELKI D IESHG  ITL QLSS+L CSP  LH
Sbjct: 15  EEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSITLSQLSSSLSCSPLLLH 74

Query: 129 RVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHK 188
           R+LRFLVHRGIFKEE+++    K Y+ TPMSRLLT++  +SMAP+ LLES+PVM+APWH 
Sbjct: 75  RILRFLVHRGIFKEEKSS----KSYSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHN 134

Query: 189 LSAFLK----DDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
           LSA +K    ++ + PFE AHG DLW+YAAA+PS + LLNE MACFARL I+PAV+ERCG
Sbjct: 135 LSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLFILPAVLERCG 194

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           EIF+GVG LVDVGGGNGTCLSILVKA PWI+GINFDLPHVV VS  YEGV+HVGG+MFD 
Sbjct: 195 EIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGVQHVGGDMFDS 254

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQ--NKLLDVR 368
           VPKADAAFIMEVLHDWDDEECIK+L+NCKEAIP+KTGKVIIVEIVIDEKE   +K  DV+
Sbjct: 255 VPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKEDQISKYSDVK 314

Query: 369 LMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           L MDMIMMAHT KGKERT EEWA +LQKAGFS Y ITPT+TIQSVIQAFP
Sbjct: 315 LRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

[Close]

BLAST of Moc04g04440 vs. ExPASy TrEMBL
Match: A0A1S3BHY1 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490251 PE=3 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 3.1e-144
Identity = 261/350 (74.57%), Postives = 300/350 (85.71%), Query Frame = 0


Query: 69  QEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSLH 128
           +E++S+ + QVWKYIFGFVEMAV+KCAIELKI D IESHG  +TL QLSS+L CSP  LH
Sbjct: 15  EEENSDIKVQVWKYIFGFVEMAVVKCAIELKIGDVIESHGDSMTLSQLSSSLSCSPLLLH 74

Query: 129 RVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWHK 188
           R+LRFLVHRGIFKEE+++    K Y+ TPMSRLLT++  +SMAP+ LLES+PVM+APWH 
Sbjct: 75  RILRFLVHRGIFKEEKSS----KSYSHTPMSRLLTTTGPNSMAPIHLLESSPVMIAPWHN 134

Query: 189 LSAFLK----DDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCG 248
           LSA +K    ++ + PFE AHG DLW+YAAA+PS + LLNE MACFARL I+PAV+ERCG
Sbjct: 135 LSACVKANGHENGSQPFEMAHGMDLWTYAAANPSHSSLLNEGMACFARLFILPAVLERCG 194

Query: 249 EIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDC 308
           EIF+GVG LVDVGGGNGTCLSILVKA PWI+GINFDLPHVV VS  YEGV+HVGG+MFD 
Sbjct: 195 EIFDGVGCLVDVGGGNGTCLSILVKACPWIKGINFDLPHVVSVSKEYEGVQHVGGDMFDS 254

Query: 309 VPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQ--NKLLDVR 368
           VPKADAAFIMEVLHDWDDEECIK+L+NCKEAIP+KTGKVIIVEIVIDEKE   +K  DV+
Sbjct: 255 VPKADAAFIMEVLHDWDDEECIKILRNCKEAIPEKTGKVIIVEIVIDEKEDQISKYSDVK 314

Query: 369 LMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAFP 413
           L MDMIMMAHT KGKERT EEWA +LQKAGFS Y ITPT+TIQSVIQAFP
Sbjct: 315 LRMDMIMMAHTIKGKERTNEEWANLLQKAGFSYYTITPTTTIQSVIQAFP 360

[Close]

BLAST of Moc04g04440 vs. ExPASy TrEMBL
Match: A0A6J1GLV0 (xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455162 PE=3 SV=1)

HSP 1 Score: 520.4 bits (1339), Expect = 6.9e-144
Identity = 255/346 (73.70%), Postives = 296/346 (85.55%), Query Frame = 0


Query: 68  EQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLPQLSSALKCSPPSL 127
           E+E++++ + QVWKYIFGFVEMAVIKCAIELKI D IE+HG  +TL  LSS+L CSP  L
Sbjct: 14  EEEENNDIKVQVWKYIFGFVEMAVIKCAIELKIGDTIEAHGGLMTLTDLSSSLNCSPLLL 73

Query: 128 HRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWH 187
           HR+LRFL+HRGIFKEE T     K Y QTPMSRLLTSS   S+AP+ +LES+ VMLAPWH
Sbjct: 74  HRILRFLIHRGIFKEEPT-----KSYAQTPMSRLLTSSGAHSLAPMHVLESSHVMLAPWH 133

Query: 188 KLSAFLKDDD--TPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCGE 247
            LSA+LK +D  + PFE AHG+DLW Y AA+ S   ++NE MA FAR+VIVPAV+ERCG 
Sbjct: 134 NLSAYLKANDGGSQPFEMAHGKDLWRYCAANSSHNKVMNEGMASFARVVIVPAVLERCGG 193

Query: 248 IFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDCV 307
           +FEGVGSLVDVGGGNG+CLS+LVKAFPWIRGINFDLPHVV VS  YEGV+HVGGNMFD V
Sbjct: 194 VFEGVGSLVDVGGGNGSCLSVLVKAFPWIRGINFDLPHVVSVSQEYEGVRHVGGNMFDFV 253

Query: 308 PKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLMM 367
           PKADAAFIM  LHDWDDE+CIK+LKNCKEAIP+KTGKVIIVE+VIDEK+++ + D+RLM+
Sbjct: 254 PKADAAFIMNALHDWDDEDCIKILKNCKEAIPEKTGKVIIVEVVIDEKDESMVSDIRLML 313

Query: 368 DMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAF 412
           DMIMMAHT KGKERTREEWAYVLQ+AGFSR+ ITPTSTIQS+IQ F
Sbjct: 314 DMIMMAHTIKGKERTREEWAYVLQQAGFSRHTITPTSTIQSIIQGF 354

[Close]

BLAST of Moc04g04440 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 358.6 bits (919), Expect = 6.7e-99
Identity = 181/362 (50.00%), Postives = 251/362 (69.34%), Query Frame = 0


Query: 66  REEQEDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGS--PITLPQLSSALKCS 125
           R+  ++ ++A   +WKY+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LSSA+  S
Sbjct: 20  RKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLAELSSAVSAS 79

Query: 126 PPSLHRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSR--LLTSSSKSSMAPLLLLESNPV 185
           P  L R++RFLVH+GIFKE  T      GY  TP+SR  ++T     S+AP +L E+ P 
Sbjct: 80  PSHLRRIMRFLVHQGIFKEIPTKDGLATGYVNTPLSRRLMITRRDGKSLAPFVLFETTPE 139

Query: 186 MLAPWHKLSAFLKD----DDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVP 245
           MLAPW +LS+ +         PPF+A HG+D+WS+A  +P  + ++NEAMAC AR V VP
Sbjct: 140 MLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINEAMACDARRV-VP 199

Query: 246 AVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHV 305
            V   C  +F+GV ++VDVGGG G  + +LVK FPWI+G NFDLPHV+ V+   +GV++V
Sbjct: 200 RVAGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIKGFNFDLPHVIEVAEVLDGVENV 259

Query: 306 GGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNK 365
            G+MFD +P  DA FI  VLHDW D++CIK+LKNCKEA+P   GKV+IVE VI E ++  
Sbjct: 260 EGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKVLIVESVIGENKKTM 319

Query: 366 LLD--------VRLMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQ 412
           ++D        VRLM+DM+MMAHT+ GKERT +EW +VL++AGF+RY +     +QS+I 
Sbjct: 320 IVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFARYEVRDIDDVQSLII 379

[Close]

BLAST of Moc04g04440 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 303.5 bits (776), Expect = 2.5e-82
Identity = 160/352 (45.45%), Postives = 220/352 (62.50%), Query Frame = 0


Query: 70  EDHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGS--PITLPQLSSALKCSPPSL 129
           ++ ++A   +W+Y+FGF ++A  KCAI+LKI +AIE+H S  P+TL +LSSA+  SP  L
Sbjct: 10  DEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSAVSASPSHL 69

Query: 130 HRVLRFLVHRGIFKEEETAIEGVKGYTQTPMSRLLTSSSKSSMAPLLLLESNPVMLAPWH 189
            R++RFLVH+G+FKE  T      GYT TP+SR +  +                      
Sbjct: 70  RRIMRFLVHQGLFKEVPTKDGLATGYTNTPLSRRMMITK--------------------- 129

Query: 190 KLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCGEIF 249
                            HG+DLW++A  +   + L+NEAMAC AR V VP V   C  +F
Sbjct: 130 ----------------LHGKDLWAFAQDNLCHSQLINEAMACDARRV-VPRVAGACQGLF 189

Query: 250 EGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDCVPK 309
           +GV ++VDVGGG G  + ILVK FPWI+G NFDLPHV+ V+   +GV++V G+MFD +P 
Sbjct: 190 DGVATVVDVGGGTGETMGILVKEFPWIKGFNFDLPHVIEVAQVLDGVENVEGDMFDSIPA 249

Query: 310 ADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVIIVEIVIDEKE--------QNKLL 369
           +DA  I  VLHDW D++CIK+LKNCKEA+    GKV+IVE VI EK+         +KL 
Sbjct: 250 SDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKNTMIAEERDDKLE 309

Query: 370 DVRLMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTSTIQSVIQAF 412
            VRL +DM+MM HT+ GKERT +EW +VL +AGF+RY +     +QS+I A+
Sbjct: 310 HVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFARYEVRDFDDVQSLIIAY 323

[Close]

BLAST of Moc04g04440 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 190.7 bits (483), Expect = 2.4e-48
Identity = 110/311 (35.37%), Postives = 177/311 (56.91%), Query Frame = 0


Query: 92  IKCAIELKIDDAIESHGSPITLPQLSSALKC----SPPSLHRVLRFLVHRGIFKEEETAI 151
           +K A+EL + + +  +GSP++  +++S L      +P  L R+LR L    +       +
Sbjct: 36  LKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVMLDRILRLLTSYSVLTCSNRKL 95

Query: 152 EG---VKGYTQTPMSRLLTSSSKS-SMAPLLLLESNPVMLAPWHKLSAFLKDDDTPPFEA 211
            G    + Y   P+ + LT +    S+A L L+  + V++  W+ L   + D    PF  
Sbjct: 96  SGDGVERIYGLGPVCKYLTKNEDGVSIAALCLMNQDKVLMESWYHLKDAILDGGI-PFNK 155

Query: 212 AHGRDLWSYAAADPSFTMLLNEAMACFARLVIVPAVIERCGEIFEGVGSLVDVGGGNGTC 271
           A+G   + Y   DP F  + N  M+  + + +   +    G  FEG+ SLVDVGGG G  
Sbjct: 156 AYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILETYKG--FEGLTSLVDVGGGIGAT 215

Query: 272 LSILVKAFPWIRGINFDLPHVVLVSPNYEGVKHVGGNMFDCVPKADAAFIMEVLHDWDDE 331
           L ++V  +P ++GINFDLPHV+  +P++ G++HVGG+MF  VPK DA F+  + HDW DE
Sbjct: 216 LKMIVSKYPNLKGINFDLPHVIEDAPSHPGIEHVGGDMFVSVPKGDAIFMKWICHDWSDE 275

Query: 332 ECIKLLKNCKEAIPKKTGKVIIVEIVIDEKEQNKLLDVRLM-MDMIMMAHTNKGKERTRE 391
            C+K LKNC E++P + GKVI+ E ++ E   + L   +++ +D IM+AH   GKERT +
Sbjct: 276 HCVKFLKNCYESLP-EDGKVILAECILPETPDSSLSTKQVVHVDCIMLAHNPGGKERTEK 335

Query: 392 EWAYVLQKAGF 394
           E+  + + +GF
Sbjct: 336 EFEALAKASGF 342

[Close]

BLAST of Moc04g04440 vs. TAIR 10
Match: AT1G77530.1 (O-methyltransferase family protein )

HSP 1 Score: 183.0 bits (463), Expect = 5.0e-46
Identity = 132/368 (35.87%), Postives = 201/368 (54.62%), Query Frame = 0


Query: 63  GMSREEQE-DHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAI--ESHGSPITLPQLSSA 122
           G+++EEQE D      Q    +       V+K A+EL + D I   S+G+ ++  +++ +
Sbjct: 16  GLTKEEQEIDEKMVSLQAESIVNAVAFPMVLKAALELGVIDTIAAASNGTWLSPSEIAVS 75

Query: 123 LKCSPPS------LHRVLRFLVHRGIFK--EEETAIEGVKG-----YTQTPMSR--LLTS 182
           L   P +      L R+LR LV   I K    E+   G  G     Y   P+ +  L  S
Sbjct: 76  LPNKPTNPEAPVLLDRMLRLLVSHSILKCCMVESRENGQTGKIERVYAAEPICKYFLKDS 135

Query: 183 SSKSSMAPLLLLESNPVMLAPWHKL-SAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLL 242
               S++ LLLL  + V+L  W  L    L+  D   F +AH   L+ Y ++D  F+ L 
Sbjct: 136 DGSGSLSSLLLLLHSQVILKTWTNLKDVILEGKDA--FSSAHDMRLFEYISSDDQFSKLF 195

Query: 243 NEAMACFARLVIVPAVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPH 302
           + AM+  + +V+   + E  G  FE V +LVDVGGG GT L ++   +P I+G+NFDL  
Sbjct: 196 HRAMSESSTMVMKKVLEEYRG--FEDVNTLVDVGGGIGTILGLITSKYPHIKGVNFDLAQ 255

Query: 303 VVLVSPNYEGVKHVGGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKV 362
           V+  +P Y GVKHV G+MF  VPK DA F+  +LHDW DE+CIK+LKNC +++P+K GKV
Sbjct: 256 VLTQAPFYPGVKHVSGDMFIEVPKGDAIFMKWILHDWGDEDCIKILKNCWKSLPEK-GKV 315

Query: 363 IIVEIV--IDEKEQNKLLDVRLMMDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPT 410
           IIVE++  ++ K  +   +  L MD++M+   + GKER+  ++  +   +GF    I   
Sbjct: 316 IIVEMITPMEPKPNDFSCNTVLGMDLLMLTQCSGGKERSLSQFENLAFASGFLLCEIICL 375

[Close]

BLAST of Moc04g04440 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 175.3 bits (443), Expect = 1.0e-43
Identity = 125/366 (34.15%), Postives = 189/366 (51.64%), Query Frame = 0


Query: 64  MSREEQE-DHSEARTQVWKYIFGFVEMAVIKCAIELKIDDAIESHGSPITLP--QLSSAL 123
           +++EEQE D      Q    +       V+K A EL + D I + G+   L   +++ +L
Sbjct: 17  LTKEEQEVDEKMVSLQAESIVNTVAFPMVLKAAFELGVIDTIAAAGNDTWLSPCEIACSL 76

Query: 124 KCSPPS------LHRVLRFLVHRGIFKEE--ETAIEGVKG-----YTQTPMSR--LLTSS 183
              P +      L R+L  LV   I K    ET   G  G     Y   P+ +  L  S 
Sbjct: 77  PTKPTNPEAPVLLDRMLSLLVSHSILKCRMIETGENGRTGKIERVYAAEPVCKYFLRDSD 136

Query: 184 SKSSMAPLLLLESNPVMLAPWHKLSAFLKDDDTPPFEAAHGRDLWSYAAADPSFTMLLNE 243
              S+ PL +L    V    W  L   +  +    F +AHG  ++ Y  +D  F  L N 
Sbjct: 137 GTGSLVPLFMLLHTQVFFKTWTNLKDVIL-EGRDAFNSAHGMKIFEYINSDQPFAELFNR 196

Query: 244 AMACFARLVIVPAVIERCGEIFEGVGSLVDVGGGNGTCLSILVKAFPWIRGINFDLPHVV 303
           AM+  + +++   +    G  FE V +LVDVGGGNGT L ++   +P I+G+NFDL  V+
Sbjct: 197 AMSEPSTMIMKKVLDVYRG--FEDVNTLVDVGGGNGTVLGLVTSKYPHIKGVNFDLAQVL 256

Query: 304 LVSPNYEGVKHVGGNMFDCVPKADAAFIMEVLHDWDDEECIKLLKNCKEAIPKKTGKVII 363
             +P Y GV+HV G+MF  VPK DA F+  +LHDW DE+CIK+LKNC +++P+K GK+II
Sbjct: 257 TQAPFYPGVEHVSGDMFVEVPKGDAVFMKWILHDWGDEDCIKILKNCWKSLPEK-GKIII 316

Query: 364 VEIVIDEKEQNKLLDVRLM--MDMIMMAHTNKGKERTREEWAYVLQKAGFSRYIITPTST 410
           VE V  ++ +   L    +  MD++M+   + GKER+  ++  +   +GF R  I   + 
Sbjct: 317 VEFVTPKEPKGGDLSSNTVFAMDLLMLTQCSGGKERSLSQFENLAFASGFLRCEIICLAY 376

The following BLAST results are available for this feature:

BLAST of Moc04g04440 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022135686.1	1.1e-196	99.43	(R,S)-reticuline 7-O-methyltransferase-like [Momordica charantia]	[more]
XP_004144871.1	6.2e-147	76.50	acetylserotonin O-methyltransferase [Cucumis sativus] >KGN43290.1 hypothetical p...	[more]
KAA0040348.1	2.9e-144	74.86	(RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK233...	[more]
XP_008447905.1	6.4e-144	74.57	PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo]	[more]
KAG6572137.1	1.1e-143	73.70	Acetylserotonin O-methyltransferase, partial [Cucurbita argyrosperma subsp. soro...	[more]

BLAST of Moc04g04440 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	9.4e-98	50.00	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
Q6WUC2	9.1e-77	43.91	(R,S)-reticuline 7-O-methyltransferase OS=Papaver somniferum OX=3469 GN=7OMT PE=...	[more]
Q9LEL6	6.1e-73	41.55	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1	[more]
Q7XB10	4.4e-71	41.38	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu...	[more]
Q5C9L7	7.5e-71	39.66	(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=...	[more]

BLAST of Moc04g04440 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C1G3	5.4e-197	99.43	(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A0A0K0Q2	3.0e-147	76.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018720 PE=3 SV=1	[more]
A0A5D3DI94	1.4e-144	74.86	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3BHY1	3.1e-144	74.57	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A6J1GLV0	6.9e-144	73.70	xanthohumol 4'-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Moc04g04440 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	6.7e-99	50.00	O-methyltransferase family protein	[more]
AT4G35150.1	2.5e-82	45.45	O-methyltransferase family protein	[more]
AT5G54160.1	2.4e-48	35.37	O-methyltransferase 1	[more]
AT1G77530.1	5.0e-46	35.87	O-methyltransferase family protein	[more]
AT1G77520.1	1.0e-43	34.15	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 62..162 e-value: 6.1E-28 score: 98.5
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 91..135 e-value: 6.6E-9 score: 35.6
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 59..412 e-value: 4.6E-123 score: 408.6
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 66..410
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 80..412 score: 77.799507
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 186..394 e-value: 2.5E-54 score: 183.8
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 164..412 e-value: 1.5E-75 score: 255.5
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 90..410 e-value: 1.7E-89 score: 297.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 55..72
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 35..53
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..72
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 66..410
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 167..408
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 69..166

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g04440.1	Moc04g04440.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity