Moc04g02670 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc04g02670
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase III domain-containing protein
Location	chr4: 1688648 .. 1691433 (-)
RNA-Seq Expression	Moc04g02670
Synteny	Moc04g02670

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGAACACGGAGTCGAGCTCTTTGAACAACTCCGCCTGCAGGCACAAGGCGCAGTGGTCAATGACGACGTGCGGCGGAATCCGCCGGAGAGTAGTGAGTCGCCGCCGAGTCTGGAGGAAGTGGAGGCGATTCTCGGCTACGAGTTCCGGGATAAGCGGTTGCTCGAAGAGGCTTTCACGGATTCCTCGTATTCGCCGGAAAATTGCTTCTCCTTCGAGCGATTGGAGTACGTCGGCGACTCGGTGTTGAATTTCTTGATCACGAGAGAGCAGTACTTCTCGAATCCGAGTTTATCGCCCGGGTCGCTGACTCGACTCAGAGCTGCGAATGTTGACACGGAAAAACTCGCTCGAGTTGCGATAACGCACGGATTCCATCGGTATCTTCGCCACAACAAATCCGATCTCGACAAGAAAGTAAGTGATTTCATTCTTGTTTATGAATTATGATCCACTCTCATTAACTTCGAATTTAACTCCTCCTCCTCGGGACTCTGCGCAAATTCTAGGTTTTATTCATCTGTTTATTTAACCCCAATTTGATTGATAATCATTTTTTTTTTAGCTTTCAGATTTTTAAATTTTAGCTTAGTTTTTTCCTGGACGTTTTATATAAATATGGTAGGAAAATTATACTAAATGGCATAATAAATATATATTTATAAAAGTTCCATTTTTTTTTAAATCATAAAAGTGAGTTTAGTTAATCCCAATGTTCTTTACATTTTATAAATATTTCAAAAATATCTTTTGAATAAATACATGTTCATATTTTGATATTAATGAAAAATTAGGCGTGTCAAAATATGAAACGGGATAAGTCAACATTAGGTACACAATCTCATTACTATCGCTTCAGATTTTTCCATGACAACTATTGAAAAAGTTATTTTTGAAACTTTTATCTAATATTAGAATTTTAGGATTGGGGTATTTTTCAAACAAATGGTAAAAGTAATTAGTTTTTTTTTTGTAATTTAGCCTATTTTATTTATACTTTTCAAGTTTTTTAAAAATGAAATTTGGTAGGAATACCTCATTTTCCTAAGATAATTATTTGAAAATTTTGTTTGACAATTTTTTTTAATAAATTCACATATCTCTAAAACTGAATAAATATGATTTATGCTACAATATGTTTTAAACAAAGATTCTTTGATATTTTTTGGTCCAAAATTGGTTATTGAAAACAAAGATTTTAGTAGGAAAAAGAAGATTTTTTTTCCCTTTTTGGAAAACATAATTGGTTGTAAATGGAAAACTTTAAGTTTATTTATTTTCTAAGAAAATAAATAAGGTGTTTAATAGAAAGAATACGTTTCTAAAGGAAAATAAATGTAATATAATTATTTTTAGTTATTGTAAATAGTAAATTGATTGTTTCTCTTGTTTTTTTTTTAGTATAATAGTAAATTGATTGCTAACAATATTTAAGAGTTACTTGTGAAAATAGGCGTGTCAGTTGTCACTGTTTTCACAATATTTTATGTTTTTAAAACATTAAATTTAAAAAAAAAATCAAGACCTTATTAAAGTAGTGGTAAAACCATATATTCAAATAATTATGTCCTTTAAAGTTAGTTAATATTTGTTTAACACTTTACGCACGAAAGTAAATTAAATAGGCAGGATGAAATATCGAAGCCTAGTGAAAATTGAATATGGTTAATCCACTTTAACCTTTTTTTATTTATTTTTTGGTTGAAATCCTTAAAAGGTTTGGACTTCCACTTTGATTCTTTTGAATGATATGATAAAGGAATTAAAGTTTATTTTTGTGGATCAGATTGCAATTTCAATTGTCAAGTTTGTAAGTCAACGTTCTCCTTACTATAAAAAGAAAATAATTTTTTTAAATAACATAAAAGGCTAATGACAACATCAATGACGTGTGATTGTTTTGAGTATATATTTTATATTTATTAAAATAATATATACATATATATTTCTAAGTTGTGTATTTTTATTTCTTCATTTGTCTCGTTGCTCATTATCTATCACTATCTAGTTATTGAGTAGAAGTTTGATGAATAATTTGGTACAGATTGGTCTGTATCATCACAATTAATGTTTGACAAAACAAGAGAAATAAAACACTATGGCAGAGATATTAGGTATCTTTAATTCATCAGCTTAGCTTATATCGTTGAGTTGATCAGTAATAATTTAACGTATCAAAACAATAAGTCATATGTTTAAACTCCTATAATGTTATTTCCTCGCGGCATTTAACATTGATTACTCGTTGGACTTTGAAAAAATTGATAGATAGAAGAGTTTGCAGAAGGGATGAAGGAGTATCCACTACATTCCAATGGACTAATCAAAGCTCCAAAAATTCTAGCGGACATTGTGGAATCAACTATTGGAGCTGTTTTCACCGACAGTAACTCTATGGACACAGTTTGGACGGTAAATATATCAATGAGAACAAAACTTGTATTATATTCTTATTGAATCATCATTATCAGTTTTGGTAACGAATGATTTAAACTGTAATGTAGATATTTAGAGGTCTAATGGAGCCATTGATAACTTTGAAGACTATAAAAAGACATCCAATGACGGAGTTAACAGAAATTTGCCAAAAAAGAAATCTGAAGCTTGAATTTGTGGACTCATGGGAGAAAACAAAGGAGGTTCAAGTTTTCATAGACAAACAGCTCGTGGGAAAAGGGAGTTACAGGAAAAAGCTTATTGCCCAAAATCGAGCTGCCAAAGATGCTTTGAACAACTTGGACACACTTCTTGTACAAATTAATCAACCTTGTCAACCTATGTAA

mRNA sequence

ATGGAAGAACACGGAGTCGAGCTCTTTGAACAACTCCGCCTGCAGGCACAAGGCGCAGTGGTCAATGACGACGTGCGGCGGAATCCGCCGGAGAGTAGTGAGTCGCCGCCGAGTCTGGAGGAAGTGGAGGCGATTCTCGGCTACGAGTTCCGGGATAAGCGGTTGCTCGAAGAGGCTTTCACGGATTCCTCGTATTCGCCGGAAAATTGCTTCTCCTTCGAGCGATTGGAGTACGTCGGCGACTCGGTGTTGAATTTCTTGATCACGAGAGAGCAGTACTTCTCGAATCCGAGTTTATCGCCCGGGTCGCTGACTCGACTCAGAGCTGCGAATGTTGACACGGAAAAACTCGCTCGAGTTGCGATAACGCACGGATTCCATCGGTATCTTCGCCACAACAAATCCGATCTCGACAAGAAAATAGAAGAGTTTGCAGAAGGGATGAAGGAGTATCCACTACATTCCAATGGACTAATCAAAGCTCCAAAAATTCTAGCGGACATTGTGGAATCAACTATTGGAGCTGTTTTCACCGACAGTAACTCTATGGACACAGTTTGGACGATATTTAGAGGTCTAATGGAGCCATTGATAACTTTGAAGACTATAAAAAGACATCCAATGACGGAGTTAACAGAAATTTGCCAAAAAAGAAATCTGAAGCTTGAATTTGTGGACTCATGGGAGAAAACAAAGGAGGTTCAAGTTTTCATAGACAAACAGCTCGTGGGAAAAGGGAGTTACAGGAAAAAGCTTATTGCCCAAAATCGAGCTGCCAAAGATGCTTTGAACAACTTGGACACACTTCTTGTACAAATTAATCAACCTTGTCAACCTATGTAA

Coding sequence (CDS)

ATGGAAGAACACGGAGTCGAGCTCTTTGAACAACTCCGCCTGCAGGCACAAGGCGCAGTGGTCAATGACGACGTGCGGCGGAATCCGCCGGAGAGTAGTGAGTCGCCGCCGAGTCTGGAGGAAGTGGAGGCGATTCTCGGCTACGAGTTCCGGGATAAGCGGTTGCTCGAAGAGGCTTTCACGGATTCCTCGTATTCGCCGGAAAATTGCTTCTCCTTCGAGCGATTGGAGTACGTCGGCGACTCGGTGTTGAATTTCTTGATCACGAGAGAGCAGTACTTCTCGAATCCGAGTTTATCGCCCGGGTCGCTGACTCGACTCAGAGCTGCGAATGTTGACACGGAAAAACTCGCTCGAGTTGCGATAACGCACGGATTCCATCGGTATCTTCGCCACAACAAATCCGATCTCGACAAGAAAATAGAAGAGTTTGCAGAAGGGATGAAGGAGTATCCACTACATTCCAATGGACTAATCAAAGCTCCAAAAATTCTAGCGGACATTGTGGAATCAACTATTGGAGCTGTTTTCACCGACAGTAACTCTATGGACACAGTTTGGACGATATTTAGAGGTCTAATGGAGCCATTGATAACTTTGAAGACTATAAAAAGACATCCAATGACGGAGTTAACAGAAATTTGCCAAAAAAGAAATCTGAAGCTTGAATTTGTGGACTCATGGGAGAAAACAAAGGAGGTTCAAGTTTTCATAGACAAACAGCTCGTGGGAAAAGGGAGTTACAGGAAAAAGCTTATTGCCCAAAATCGAGCTGCCAAAGATGCTTTGAACAACTTGGACACACTTCTTGTACAAATTAATCAACCTTGTCAACCTATGTAA

Protein sequence

MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM

Homology

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BLAST of Moc04g02670 vs. NCBI nr
Match: XP_022136054.1 (ribonuclease 3-like protein 3 [Momordica charantia])

HSP 1 Score: 556.2 bits (1432), Expect = 1.6e-154
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF 60
           MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF
Sbjct: 1   MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF 60

Query: 61  TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV 120
           TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV
Sbjct: 61  TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV 120

Query: 121 AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS 180
           AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS
Sbjct: 121 AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS 180

Query: 181 NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK 240
           NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK
Sbjct: 181 NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK 240

Query: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 281
           QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM
Sbjct: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 280

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BLAST of Moc04g02670 vs. NCBI nr
Match: XP_038888348.1 (ribonuclease 3-like protein 3 [Benincasa hispida])

HSP 1 Score: 426.0 bits (1094), Expect = 2.5e-115
Identity = 216/279 (77.42%), Postives = 236/279 (84.59%), Query Frame = 0


Query: 2   EEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAFT 61
           EE  V   E+      G  VN           +S  SL+ +EAIL YEFRDKRLLEEAFT
Sbjct: 4   EEQRVVFLEEEGSSGGGEAVN---------GGDSAASLDGIEAILNYEFRDKRLLEEAFT 63

Query: 62  DSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARVA 121
           D+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP+LSPGSLTRLRAANVDTEKLARVA
Sbjct: 64  DASYSPENCSSFERLEYVGDSVLNFLITREQYFSNPNLSPGSLTRLRAANVDTEKLARVA 123

Query: 122 ITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDSN 181
           ITHGFHRYLRHNK DL  KIEEF + +++YPLHSNG+IKAPK+LADIVESTIGAVF DSN
Sbjct: 124 ITHGFHRYLRHNKPDLHPKIEEFIKSIEDYPLHSNGMIKAPKVLADIVESTIGAVFADSN 183

Query: 182 SMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDKQ 241
           S++TVW IF+ LMEPLITLKT+K+HPMTELTEICQKRNL LEFVD WE+ KEVQVFIDKQ
Sbjct: 184 SIETVWKIFKDLMEPLITLKTMKKHPMTELTEICQKRNLNLEFVDCWEERKEVQVFIDKQ 243

Query: 242 LVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 281
           LVGKGSYRKKLIAQNRAAKDALNNLD +L + NQPCQPM
Sbjct: 244 LVGKGSYRKKLIAQNRAAKDALNNLDRVLGEFNQPCQPM 273

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BLAST of Moc04g02670 vs. NCBI nr
Match: XP_023554575.1 (ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 424.9 bits (1091), Expect = 5.5e-115
Identity = 209/243 (86.01%), Postives = 228/243 (93.83%), Query Frame = 0


Query: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217
           LIKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263

Query: 278 QPM 281
           QPM
Sbjct: 264 QPM 266

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BLAST of Moc04g02670 vs. NCBI nr
Match: XP_022969213.1 (ribonuclease 3-like protein 3 [Cucurbita maxima])

HSP 1 Score: 424.1 bits (1089), Expect = 9.4e-115
Identity = 208/243 (85.60%), Postives = 228/243 (93.83%), Query Frame = 0


Query: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217
           +IKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 MIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263

Query: 278 QPM 281
           QPM
Sbjct: 264 QPM 266

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BLAST of Moc04g02670 vs. NCBI nr
Match: KAG6572281.1 (Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 423.7 bits (1088), Expect = 1.2e-114
Identity = 208/243 (85.60%), Postives = 228/243 (93.83%), Query Frame = 0


Query: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157
           +LSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 84  NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217
           LIKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263

Query: 278 QPM 281
           QPM
Sbjct: 264 QPM 266

[Close]

BLAST of Moc04g02670 vs. ExPASy Swiss-Prot
Match: Q69KJ0 (Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0358800 PE=2 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 4.8e-61
Identity = 123/242 (50.83%), Postives = 169/242 (69.83%), Query Frame = 0


Query: 36  PPSLEEVEAILGYEFRDKRLLEEAFTDSS--YSPENCFSFERLEYVGDSVLNFLITREQY 95
           P  +  VE++LGYEFRDK L+EEA T  S  Y      ++ERLEY+GD+VL  +++RE +
Sbjct: 36  PGEVAAVESLLGYEFRDKALVEEALTHGSFYYPYRPGVTYERLEYLGDAVLTCVVSREVF 95

Query: 96  FSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPL 155
            +   L PG LTRLRAANVD EKLARVA+ HG H +LRH   +LD +I +F E +  YP+
Sbjct: 96  LTYGQLQPGPLTRLRAANVDKEKLARVAVVHGLHHFLRHKAPNLDGQITDFIEELSMYPI 155

Query: 156 HSNGLIKAPKILADIVESTIGAVFTDSN-SMDTVWTIFRGLMEPLITLKTIKRHPMTELT 215
           HSNGL+  PK+L D+VES IGA++ DSN + + VW +F+ L +PLI+L+T+ +HP++EL 
Sbjct: 156 HSNGLLDPPKVLCDVVESLIGAIYCDSNFNQEIVWQVFQKLADPLISLETLGKHPVSELF 215

Query: 216 EICQKRNLKLEFV-DSWEKTKEVQVFIDKQLVGKGSY-RKKLIAQNRAAKDALNNLDTLL 273
           E CQK    ++ V D W+K   V+V ID ++VG+ +Y +KK IAQNRAAK AL+ L   L
Sbjct: 216 EFCQKTRRGVKIVKDEWDKNLTVEVLIDGEMVGRATYAQKKEIAQNRAAKAALDKLKETL 275

[Close]

BLAST of Moc04g02670 vs. ExPASy Swiss-Prot
Match: Q6ATG6 (Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0271300 PE=2 SV=2)

HSP 1 Score: 179.9 bits (455), Expect = 4.1e-44
Identity = 107/261 (41.00%), Postives = 154/261 (59.00%), Query Frame = 0


Query: 17  QGAVVNDDVRRNPPE-SSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFER 76
           +G    D V   PP   ++   +   VE +L Y+FRD RLLEEA T  S++ ++  S++R
Sbjct: 14  RGPPAPDPVELPPPGFVADRAEAAARVERLLRYQFRDGRLLEEALTHQSFA-DDAVSYQR 73

Query: 77  LEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKS 136
           LE+VGDS L    +   Y +NP+L PG L+ LRAAN+ TEKLARVA+ H  +  LR N  
Sbjct: 74  LEFVGDSALGLAFSNFLYLTNPTLGPGPLSTLRAANISTEKLARVAVRHDLYPLLRRNCP 133

Query: 137 DLDKKIEEFAEGMKEYP-------LHSNGLIKAPKILADIVESTIGAVFTDSN-SMDTVW 196
            LD  + +F E +K+ P        +   ++KAPK+LADIVE+   AV+ D    ++ +W
Sbjct: 134 RLDLLVGQFIETVKQEPEDDLSTVPYGGSVVKAPKVLADIVEAIAAAVYVDCKFDLEKLW 193

Query: 197 TIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEK--TKEVQVFIDKQLVGK 256
            + R L EP+IT +TI   P+T L E+CQK     +F  +W+K     V VF+  +LVG 
Sbjct: 194 KVTRWLFEPIITAETIDEQPVTMLHELCQKHGKMAQF-KTWQKGGMTVVNVFVAGELVGI 253

Query: 257 GSYRKKLIAQNRAAKDALNNL 267
           GS  +K+IA+  AA+DA   L
Sbjct: 254 GSSEQKVIAKLNAARDATRKL 272

[Close]

BLAST of Moc04g02670 vs. ExPASy Swiss-Prot
Match: Q9LTQ0 (Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1)

HSP 1 Score: 175.6 bits (444), Expect = 7.7e-43
Identity = 101/245 (41.22%), Postives = 155/245 (63.27%), Query Frame = 0


Query: 30  PESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLIT 89
           P SSE   S+E VE IL Y+F +K LL+EA T +S +  +  S+ERLE++GDS +   I+
Sbjct: 52  PVSSEM-ESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAIS 111

Query: 90  REQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGM- 149
              Y + PSL P  L+ LRAANV TEKLARV++ HG + +LR N   LD+K++EF+E + 
Sbjct: 112 NYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVG 171

Query: 150 --KEYPLHSNGLIKAPKILADIVESTIGAVFTDSN-SMDTVWTIFRGLMEPLITLKTIKR 209
              +  +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IFRGL+EP++TL  +++
Sbjct: 172 KEDDLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQK 231

Query: 210 --HPMTELTEICQKRNLKLEFVDSWE--KTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKD 267
              P++ L ++C K   +++ + +W+        +++D +L+  G    K IA+  AAK+
Sbjct: 232 QPQPVSMLFKLCHKHKKRID-IKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKE 291

[Close]

BLAST of Moc04g02670 vs. ExPASy Swiss-Prot
Match: Q9SP32 (Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV=2)

HSP 1 Score: 172.6 bits (436), Expect = 6.5e-42
Identity = 108/247 (43.72%), Postives = 144/247 (58.30%), Query Frame = 0


Query: 28   NPPESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFL 87
            N PES         +E  L YEF++K LL EA T +S        ++RLE+VGD+VL+ L
Sbjct: 1548 NVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHL 1607

Query: 88   ITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEG 147
            ITR  +F+  SL PG LT LRAA V+ E  ARVA+ H  H YLRH  S L+K+I EF   
Sbjct: 1608 ITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREF--- 1667

Query: 148  MKEYPLHSN---------GLIKAPKILADIVESTIGAVFTDSNSMDT-VWTIFRGLMEPL 207
            +KE    S+         G  KAPK+L DIVES  GA+F DS    T  W +F+ L++P+
Sbjct: 1668 VKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPM 1727

Query: 208  ITLKTIKRHPMTELTEICQKRNLKLEFVDSWE-KTKEVQVFIDKQLVGKGSYRKKLIAQN 264
            +T +T+  HP+ EL E CQ++   LE+  S    T  V+VFID   VG     +K +AQ 
Sbjct: 1728 VTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQK 1787

[Close]

BLAST of Moc04g02670 vs. ExPASy Swiss-Prot
Match: Q8LMR2 (Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL1 PE=3 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 2.7e-40
Identity = 101/242 (41.74%), Postives = 145/242 (59.92%), Query Frame = 0


Query: 30   PESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLIT 89
            PES     + + ++ +LG EF++K LL EA T +S        ++RLE+VGD+VL+ LIT
Sbjct: 1529 PESIMRSINFDTLKGVLGIEFQNKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHLIT 1588

Query: 90   REQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMK 149
            R  +F+   L PG LT LRAA V+ E  ARVA+ H  H +LRH  S L+ +I EF + ++
Sbjct: 1589 RHLFFTYTDLPPGRLTDLRAAAVNNENFARVAVKHKLHVHLRHGSSALETQIREFVKDVQ 1648

Query: 150  EYPL----HSNGL--IKAPKILADIVESTIGAVFTDSN-SMDTVWTIFRGLMEPLITLKT 209
            E  L    +S GL   KAPK+L DIVES  GA+F DS      VW +F+ L+ P++T +T
Sbjct: 1649 EELLKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGYDTSVVWKVFQPLLHPMVTPET 1708

Query: 210  IKRHPMTELTEICQKRNLKLEFVDSWE-KTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKD 264
            +  HP+ EL E CQ++   LE+  S       V+VF+D   +G     +K +AQ  AA++
Sbjct: 1709 LPMHPVRELQERCQQQAEGLEYKASRAGNIATVEVFVDGVQIGVAQNPQKKMAQKLAARN 1768

[Close]

BLAST of Moc04g02670 vs. ExPASy TrEMBL
Match: A0A6J1C375 (ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=4 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 7.7e-155
Identity = 280/280 (100.00%), Postives = 280/280 (100.00%), Query Frame = 0


Query: 1   MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF 60
           MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF
Sbjct: 1   MEEHGVELFEQLRLQAQGAVVNDDVRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAF 60

Query: 61  TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV 120
           TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV
Sbjct: 61  TDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARV 120

Query: 121 AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS 180
           AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS
Sbjct: 121 AITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDS 180

Query: 181 NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK 240
           NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK
Sbjct: 181 NSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDK 240

Query: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 281
           QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM
Sbjct: 241 QLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPCQPM 280

[Close]

BLAST of Moc04g02670 vs. ExPASy TrEMBL
Match: A0A6J1I0C1 (ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 4.6e-115
Identity = 208/243 (85.60%), Postives = 228/243 (93.83%), Query Frame = 0


Query: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 24  SLDGMEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157
           +LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 84  NLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217
           +IKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 MIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263

Query: 278 QPM 281
           QPM
Sbjct: 264 QPM 266

[Close]

BLAST of Moc04g02670 vs. ExPASy TrEMBL
Match: A0A6J1GKQ4 (ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4 SV=1)

HSP 1 Score: 422.9 bits (1086), Expect = 1.0e-114
Identity = 208/243 (85.60%), Postives = 228/243 (93.83%), Query Frame = 0


Query: 38  SLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNP 97
           SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQYFSNP
Sbjct: 24  SLDGMEAILKYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQYFSNP 83

Query: 98  SLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNG 157
           +LSPG+LTRLRAANVDTEKLARVAITHGFHRYLRHNK+DLD+KIEEF++ ++EYPLHSNG
Sbjct: 84  NLSPGTLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLDQKIEEFSKNIEEYPLHSNG 143

Query: 158 LIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQK 217
           LIKAPKILADIVES IGAVFTDSNS+DTVWTIF+GLMEPLITLKT+KRHPMTELTE+CQK
Sbjct: 144 LIKAPKILADIVESCIGAVFTDSNSIDTVWTIFKGLMEPLITLKTMKRHPMTELTEMCQK 203

Query: 218 RNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQINQPC 277
           RNLK EFVD WE+ KE+ V IDKQLVGKGSY KKLIAQNRAAKDAL+N  T+L Q N+PC
Sbjct: 204 RNLKWEFVDLWEERKEIHVLIDKQLVGKGSYGKKLIAQNRAAKDALDNFHTILGQFNEPC 263

Query: 278 QPM 281
           QPM
Sbjct: 264 QPM 266

[Close]

BLAST of Moc04g02670 vs. ExPASy TrEMBL
Match: A0A0A0K1J8 (RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 5.1e-114
Identity = 205/248 (82.66%), Postives = 228/248 (91.94%), Query Frame = 0


Query: 33  SESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQ 92
           S+SP SL+ +EAIL YEFRDKRLLEEAFTD+SYSPENC SFERLEYVGDSVLNFLITREQ
Sbjct: 28  SDSPASLDGIEAILNYEFRDKRLLEEAFTDASYSPENCSSFERLEYVGDSVLNFLITREQ 87

Query: 93  YFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYP 152
           YFSNP+LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNK+DL +KIEEF   ++EYP
Sbjct: 88  YFSNPNLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKADLHQKIEEFGRSIEEYP 147

Query: 153 LHSNGLIKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELT 212
           LHSNG+IKAPK+LADIVESTIGAVF DSN +DTVW IF+ LMEPLITLKT+K+HPMTELT
Sbjct: 148 LHSNGMIKAPKVLADIVESTIGAVFADSNFIDTVWKIFKELMEPLITLKTMKKHPMTELT 207

Query: 213 EICQKRNLKLEFVDSWEKTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKDALNNLDTLLVQ 272
           E+CQKR+LKLEF D WE+TKE++V I+KQ+VGKGSYRKKLIAQNRAAK+AL NLDT   Q
Sbjct: 208 EMCQKRSLKLEFRDRWEETKEIEVLIEKQVVGKGSYRKKLIAQNRAAKNALENLDTFFPQ 267

Query: 273 INQPCQPM 281
            NQPCQPM
Sbjct: 268 FNQPCQPM 275

[Close]

BLAST of Moc04g02670 vs. ExPASy TrEMBL
Match: A0A6A1UL56 (Ribonuclease 3-like protein 3 OS=Morella rubra OX=262757 GN=CJ030_MR0G005267 PE=4 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 1.8e-79
Identity = 165/256 (64.45%), Postives = 194/256 (75.78%), Query Frame = 0


Query: 21  VNDDVRRNPPES--SESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEY 80
           + +D R    ES  +ES   L EVE ILGYEF++K LLEEAFT SS+S E+CFS+ERLEY
Sbjct: 21  LQEDERELDAESTQTESLRDLHEVEEILGYEFKNKSLLEEAFTHSSFS-EDCFSYERLEY 80

Query: 81  VGDSVLNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLD 140
           VGD+VLN L T+EQYF  P L PG LTRLRAANVDTEKLAR AI HG HRYLRH K  L 
Sbjct: 81  VGDAVLNLLFTKEQYFLYPDLQPGPLTRLRAANVDTEKLARAAIKHGLHRYLRHKKPLLH 140

Query: 141 KKIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDSN-SMDTVWTIFRGLMEPL 200
            +I +F  G+ EYPLHSNGLI APK LADIVES+IGAVF DSN S+DT W +F+GL+EP+
Sbjct: 141 DQIHKFTLGISEYPLHSNGLIDAPKALADIVESSIGAVFIDSNSSIDTAWEVFKGLLEPI 200

Query: 201 ITLKTIKRHPMTELTEICQKRNLKLEFVDSWEKTKEVQVFIDKQLVGKGSY-RKKLIAQN 260
           I   TI RHP+TEL E+CQKRNLKL+FVD WE+T    V ID Q +G+G Y  KK IA N
Sbjct: 201 INPVTIGRHPVTELYEVCQKRNLKLKFVDLWEETTAFDVIIDDQHMGRGVYGLKKDIAHN 260

Query: 261 RAAKDALNNLDTLLVQ 273
           RAAK+AL N+  LL++
Sbjct: 261 RAAKNALENIRRLLLE 275

[Close]

BLAST of Moc04g02670 vs. TAIR 10
Match: AT4G15417.1 (RNAse II-like 1 )

HSP 1 Score: 239.6 bits (610), Expect = 3.1e-63
Identity = 123/228 (53.95%), Postives = 163/228 (71.49%), Query Frame = 0


Query: 39  LEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLITREQYFSNPS 98
           +E +E IL Y+F+DK LL +AFTD+SY  +   S+E LE +GDS+LN  I  +     P 
Sbjct: 29  IESLEKILNYKFKDKSLLLKAFTDASYVDDKSESYELLELLGDSILNMGIIYDFIKLYPK 88

Query: 99  LSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGMKEYPLHSNGL 158
            +PG LT+LRA NVDTEKLARVA+ H  + YLRH K  L+++I EF E M++YPLHSNGL
Sbjct: 89  EAPGPLTKLRAVNVDTEKLARVAVNHQLYSYLRHKKPLLEEQILEFVEAMEKYPLHSNGL 148

Query: 159 IKAPKILADIVESTIGAVFTDSNSMDTVWTIFRGLMEPLITLKTIKRHPMTELTEICQKR 218
           +K PK+LADIVESTIGA+F D NS +TVW + + L+EP+I L  +K HPMTEL E+CQKR
Sbjct: 149 LKVPKVLADIVESTIGAIFMDCNSTETVWKVIKPLLEPIIHLDKMKSHPMTELNEMCQKR 208

Query: 219 NLKLEFVDSWEKTKEVQVFIDKQLVGKGSYR-KKLIAQNRAAKDALNN 266
           NLKL   D+WE+ +     I+ + VG G +  KK  A+N AAK+A++N
Sbjct: 209 NLKLTSKDTWEENQTYCFHIEDKFVGCGHHPVKKETARNFAAKNAIDN 256

[Close]

BLAST of Moc04g02670 vs. TAIR 10
Match: AT3G20420.1 (RNAse THREE-like protein 2 )

HSP 1 Score: 175.6 bits (444), Expect = 5.4e-44
Identity = 101/245 (41.22%), Postives = 155/245 (63.27%), Query Frame = 0


Query: 30  PESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFLIT 89
           P SSE   S+E VE IL Y+F +K LL+EA T +S +  +  S+ERLE++GDS +   I+
Sbjct: 52  PVSSEM-ESMEAVEKILNYKFSNKSLLKEAITHTSCT--DFPSYERLEFIGDSAIGLAIS 111

Query: 90  REQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEGM- 149
              Y + PSL P  L+ LRAANV TEKLARV++ HG + +LR N   LD+K++EF+E + 
Sbjct: 112 NYLYLTYPSLEPHDLSLLRAANVSTEKLARVSLNHGLYSFLRRNAPSLDEKVKEFSEAVG 171

Query: 150 --KEYPLHSNGLIKAPKILADIVESTIGAVFTDSN-SMDTVWTIFRGLMEPLITLKTIKR 209
              +  +   GL+KAPK+LAD+ ES  GAV+ D N  +  +W IFRGL+EP++TL  +++
Sbjct: 172 KEDDLSVSYGGLVKAPKVLADLFESLAGAVYVDVNFDLQRLWVIFRGLLEPIVTLDDLQK 231

Query: 210 --HPMTELTEICQKRNLKLEFVDSWE--KTKEVQVFIDKQLVGKGSYRKKLIAQNRAAKD 267
              P++ L ++C K   +++ + +W+        +++D +L+  G    K IA+  AAK+
Sbjct: 232 QPQPVSMLFKLCHKHKKRID-IKNWKDGNVSIAVIYLDDELLASGRAENKDIARLIAAKE 291

[Close]

BLAST of Moc04g02670 vs. TAIR 10
Match: AT1G01040.1 (dicer-like 1 )

HSP 1 Score: 172.6 bits (436), Expect = 4.6e-43
Identity = 108/247 (43.72%), Postives = 144/247 (58.30%), Query Frame = 0


Query: 28   NPPESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFL 87
            N PES         +E  L YEF++K LL EA T +S        ++RLE+VGD+VL+ L
Sbjct: 1548 NVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHL 1607

Query: 88   ITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEG 147
            ITR  +F+  SL PG LT LRAA V+ E  ARVA+ H  H YLRH  S L+K+I EF   
Sbjct: 1608 ITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREF--- 1667

Query: 148  MKEYPLHSN---------GLIKAPKILADIVESTIGAVFTDSNSMDT-VWTIFRGLMEPL 207
            +KE    S+         G  KAPK+L DIVES  GA+F DS    T  W +F+ L++P+
Sbjct: 1668 VKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPM 1727

Query: 208  ITLKTIKRHPMTELTEICQKRNLKLEFVDSWE-KTKEVQVFIDKQLVGKGSYRKKLIAQN 264
            +T +T+  HP+ EL E CQ++   LE+  S    T  V+VFID   VG     +K +AQ 
Sbjct: 1728 VTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQK 1787

[Close]

BLAST of Moc04g02670 vs. TAIR 10
Match: AT1G01040.2 (dicer-like 1 )

HSP 1 Score: 172.6 bits (436), Expect = 4.6e-43
Identity = 108/247 (43.72%), Postives = 144/247 (58.30%), Query Frame = 0


Query: 28   NPPESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSYSPENCFSFERLEYVGDSVLNFL 87
            N PES         +E  L YEF++K LL EA T +S        ++RLE+VGD+VL+ L
Sbjct: 1549 NVPESVLKSIDFVGLERALKYEFKEKGLLVEAITHASRPSSGVSCYQRLEFVGDAVLDHL 1608

Query: 88   ITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYLRHNKSDLDKKIEEFAEG 147
            ITR  +F+  SL PG LT LRAA V+ E  ARVA+ H  H YLRH  S L+K+I EF   
Sbjct: 1609 ITRHLFFTYTSLPPGRLTDLRAAAVNNENFARVAVKHKLHLYLRHGSSALEKQIREF--- 1668

Query: 148  MKEYPLHSN---------GLIKAPKILADIVESTIGAVFTDSNSMDT-VWTIFRGLMEPL 207
            +KE    S+         G  KAPK+L DIVES  GA+F DS    T  W +F+ L++P+
Sbjct: 1669 VKEVQTESSKPGFNSFGLGDCKAPKVLGDIVESIAGAIFLDSGKDTTAAWKVFQPLLQPM 1728

Query: 208  ITLKTIKRHPMTELTEICQKRNLKLEFVDSWE-KTKEVQVFIDKQLVGKGSYRKKLIAQN 264
            +T +T+  HP+ EL E CQ++   LE+  S    T  V+VFID   VG     +K +AQ 
Sbjct: 1729 VTPETLPMHPVRELQERCQQQAEGLEYKASRSGNTATVEVFIDGVQVGVAQNPQKKMAQK 1788

[Close]

BLAST of Moc04g02670 vs. TAIR 10
Match: AT3G03300.1 (dicer-like 2 )

HSP 1 Score: 137.1 bits (344), Expect = 2.1e-32
Identity = 87/253 (34.39%), Postives = 138/253 (54.55%), Query Frame = 0


Query: 25   VRRNPPESSESPPSLEEVEAILGYEFRDKRLLEEAFTDSSY-SPENCFSFERLEYVGDSV 84
            ++R+ P  +E   ++  +E++L Y F DK LL EA T  SY  PE    ++RLE++GDSV
Sbjct: 1135 IQRDSPIQAEKLVNVGYMESLLNYSFEDKSLLVEALTHGSYMMPEIPRCYQRLEFLGDSV 1194

Query: 85   LNFLITREQYFSNPSLSPGSLTRLRAANVDTEKLARVAITHGFHRYL----RHNKSDLDK 144
            L++LIT+  Y   P LSPG LT +R+A+V+ E  A VA+    H+++     H    + +
Sbjct: 1195 LDYLITKHLYDKYPCLSPGLLTDMRSASVNNECYALVAVKANLHKHILYASHHLHKHISR 1254

Query: 145  KIEEFAEGMKEYPLHSNGLIKAPKILADIVESTIGAVFTDSN-SMDTVWTIFRGLMEPLI 204
             + EF +   +        I  PK+L D++ES  GA+F DS  + + V+   + L+  +I
Sbjct: 1255 TVSEFEQSSLQSTFGWESDISFPKVLGDVIESLAGAIFVDSGYNKEVVFASIKPLLGCMI 1314

Query: 205  TLKTIKRHPMTELTEICQKRNLKLEFVDSWEK-TKEVQVFIDKQLVGKGSYRKKLIAQNR 264
            T +T+K HP+ ELTE+CQK   +L     ++  T EV+   +           K +A+  
Sbjct: 1315 TPETVKLHPVRELTELCQKWQFELSKAKDFDSFTVEVKA-KEMSFAHTAKASDKKMAKKL 1374

Query: 265  AAKDALNNLDTLL 271
            A K+ LN L   L
Sbjct: 1375 AYKEVLNLLKNSL 1386

The following BLAST results are available for this feature:

BLAST of Moc04g02670 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022136054.1	1.6e-154	100.00	ribonuclease 3-like protein 3 [Momordica charantia]	[more]
XP_038888348.1	2.5e-115	77.42	ribonuclease 3-like protein 3 [Benincasa hispida]	[more]
XP_023554575.1	5.5e-115	86.01	ribonuclease 3-like protein 3 [Cucurbita pepo subsp. pepo]	[more]
XP_022969213.1	9.4e-115	85.60	ribonuclease 3-like protein 3 [Cucurbita maxima]	[more]
KAG6572281.1	1.2e-114	85.60	Endoribonuclease Dicer-like 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Moc04g02670 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q69KJ0	4.8e-61	50.83	Ribonuclease 3-like protein 3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0...	[more]
Q6ATG6	4.1e-44	41.00	Ribonuclease 3-like protein 2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0...	[more]
Q9LTQ0	7.7e-43	41.22	Ribonuclease 3-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=RTL2 PE=1 SV=1	[more]
Q9SP32	6.5e-42	43.72	Endoribonuclease Dicer homolog 1 OS=Arabidopsis thaliana OX=3702 GN=DCL1 PE=1 SV...	[more]
Q8LMR2	2.7e-40	41.74	Endoribonuclease Dicer homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=DCL...	[more]

BLAST of Moc04g02670 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C375	7.7e-155	100.00	ribonuclease 3-like protein 3 OS=Momordica charantia OX=3673 GN=LOC111007843 PE=...	[more]
A0A6J1I0C1	4.6e-115	85.60	ribonuclease 3-like protein 3 OS=Cucurbita maxima OX=3661 GN=LOC111468279 PE=4 S...	[more]
A0A6J1GKQ4	1.0e-114	85.60	ribonuclease 3-like protein 3 OS=Cucurbita moschata OX=3662 GN=LOC111455247 PE=4...	[more]
A0A0A0K1J8	5.1e-114	82.66	RNase III domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G041940 P...	[more]
A0A6A1UL56	1.8e-79	64.45	Ribonuclease 3-like protein 3 OS=Morella rubra OX=262757 GN=CJ030_MR0G005267 PE=...	[more]

BLAST of Moc04g02670 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G15417.1	3.1e-63	53.95	RNAse II-like 1	[more]
AT3G20420.1	5.4e-44	41.22	RNAse THREE-like protein 2	[more]
AT1G01040.1	4.6e-43	43.72	dicer-like 1	[more]
AT1G01040.2	4.6e-43	43.72	dicer-like 1	[more]
AT3G03300.1	2.1e-32	34.39	dicer-like 2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000999	Ribonuclease III domain	SMART	SM00535	riboneu5	coord: 54..203 e-value: 1.2E-30 score: 117.8
IPR000999	Ribonuclease III domain	PFAM	PF00636	Ribonuclease_3	coord: 74..180 e-value: 1.2E-21 score: 77.3
IPR000999	Ribonuclease III domain	PROSITE	PS00517	RNASE_3_1	coord: 74..82
IPR000999	Ribonuclease III domain	PROSITE	PS50142	RNASE_3_2	coord: 39..181 score: 34.434845
IPR000999	Ribonuclease III domain	CDD	cd00593	RIBOc	coord: 54..199 e-value: 1.83691E-39 score: 132.351
None	No IPR available	GENE3D	3.30.160.20		coord: 207..275 e-value: 1.6E-9 score: 39.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 20..39
None	No IPR available	PANTHER	PTHR14950:SF54	RNASE II-LIKE 1	coord: 27..270
None	No IPR available	PANTHER	PTHR14950	DICER-RELATED	coord: 27..270
None	No IPR available	SUPERFAMILY	54768	dsRNA-binding domain-like	coord: 163..266
IPR036389	Ribonuclease III, endonuclease domain superfamily	GENE3D	1.10.1520.10	Ribonuclease III domain	coord: 36..206 e-value: 1.2E-49 score: 170.6
IPR036389	Ribonuclease III, endonuclease domain superfamily	SUPERFAMILY	69065	RNase III domain-like	coord: 38..200
IPR014720	Double-stranded RNA-binding domain	PFAM	PF00035	dsrm	coord: 207..266 e-value: 5.6E-6 score: 27.0

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc04g02670.1	Moc04g02670.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030422	production of siRNA involved in RNA interference
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0006396	RNA processing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0004525	ribonuclease III activity
molecular_function	GO:0003723	RNA binding