Moc04g01100 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g01100
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionKetol-acid reductoisomerase
Locationchr4: 723764 .. 728337 (+)
RNA-Seq ExpressionMoc04g01100
SyntenyMoc04g01100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCGCCACATCTTTCTCCCCCGCTATCTCAGCCTCATCATCCAAAACCCTAAAGAAGGCTGCTCTCAAGCCCCCGGCTCCGGCCAGCTTAGGGTTCTTGTCGTCATCTTCTTCCATGGGATCGAAGGCTCTCAAGGCTGTTGGCGTCAGTGGTGCTAATGCCGACGGCTCGGCTCTTGGTGCTCGCATGGTGTCCGTGCCTGCGATTAAGCCCTTAACTTCGCTCGATTTTGACACATCTGTCTTCAAGAAGGAGAAAGTCAATCTCGCCGGCCACGACGAGGTTTTTAGTCTGTTGTTCATTTGTTTGTTACTGGATTATTGAGTGTTATATGGCGTTGGGGGAAGTGAGTTGAGAGTGAGTGTGAATAGGTTAACGTGCATTCATCTCCTAAGATCTGCATGCGTTTTCTTAACATATCTGGAACGTTCTGCTTTGGGGTCTTGAAGTATGGCATTTCTTGACGGAAATGTTTTTGGATTGGGAATTAGAGTTCAGTTGTACCATTTCTTATTGTTTGCCTTCGGCTTGCAGTATATTGTGAGAGGAGGTAGGGATCTTTTTCCCTTGCTGCCGGAAGCATTCAAGGGGATCAAGCAAATTGGTGTCATTGGCTGGGGTTCTCAGGTAATGTTATCTCACACGGCGTCTTTCTTCCGCAATTTTATATTTGTCATTTTTTGTTGCCTCCATATTTTGATGGTAAATAACAGTCCAATGGGTTGAGTAAATTGCTACGCTATATATATATATATATTTTTTTTTAATTTGAAATCATAAACCGCACATTTGGAAATCGTTTGTTTTACCTTAAGAGTTTATTTTTCTGTTAATACTAATAGTAAAAAATTGATGTGATATTTTACGTAATGAAGGATAAACTGGAAAACTATATTTTAAACTTTCAGCCTGCTTGCTTAGATTATCTTTGTCACGTAAGCATCTCCATTGTTATTTGCTAAAATGTTATGCGATAAAAATTAAAATGTGTAATTTCCTACGGACTAGTGGCGAGTCAACTAAGTGACAAACAGGGTACTAAAAATTTCATTTTTATAACGTAACTTTTTCAGATGTGTTAAGGAGATTATAGTTTTGAATTTTTTTGATAAATTTAGCTGATGCGTGCATCTTAATGTAGCGTGTGAGTATGGTCTAGTTCAATAAAAAAAATTTCCTGAGAAAACGAGATTATAGTATTGTTTAATTGACAGTGTTGTTGAGGTGAGCCCAGGTGCTCTCCTATGGTCTGGTGAGGTGAGACCTCAGCGCCTTGAAGGTGTCCATGGCAAACACCTTCAACGGGGCCATGCGCCATTTAGTGCCATCTCCATTAAGCAAGGCGAGTGACTTAGATGGGTACAAAGGGTTGTCTTAAAAAGGAAAAAAAGGGTTAAAGTTAGAATAATAAAAGGAAGAGTCGAAAAATGAAGAAAAAGAATGAGAATGAGAAGAAGTGACAGAATTGAAGAAGAAAGAGAAAAATAATTAGAAGAGACAGAAGAGGAAGAAGGAAAAAAAAGGGTGATGGCTGCTGCATGCAGCATTTTTTCTCCATGTTAGACAGATGACGATTCTCTTATAAATAAAAGTCTCGCAGTATTTTCCATGATTAACAGATGGCAGGAAGTTGTATTGGTGGCCAGGAGGTCGTATCTAAAGTTTTGTTTAATCTTTAGTCGTAACACTATCTTTTTTTAACCATATTAAAAAAAAAATCTCTATTATCTTTTTTCCTGAAAATCTCTACTTTTTCCTAATTATTATTTAAGTAAATTTTAATATAAATATTAAATAGTTTTGTCAATAAACTGAAGTATTTGTTTTTTACCTATACTTATTATGGATAATACTTATTTAGTATTTTATTAGGTGCATCTTGCTTCACTTAGGCGATGCCTTTTTGTCACCTCTCATCTTGAGGTGATTAGAAGACTTGCTGCCATAAGGTGCGCCTTGCGCTTTGAAAACATTGATTTTTGAGAGACATTCAGATGCATCTGAATGTAGCATGTGAATATAGCTCTAGTTCAATGAAACTTTTTCTTGAGAAAAGCATTCTGGATCTTTACTTTTTTCTCAGGAAGACAGAAATTTTTCTGAGCCTTTTTTATTGATCCTGTAGTGTACTGACCATAGTTTTATGCATGTGCATTCATTTGTTGGATCAGGGACCTGCACAGGCTCAGAATTTGAGGGACTCGCTTGCTGAAGCTAAGTCTGATATTGTGGTTAAGGTGATTTTACTTTTTCGTACATTACAATTTACTCATAAATCTATTGTACATGATTCTTCATTGGAGTGTTTATTTCCTATTGCATTGTAGGTTGTTATGTCGTATTATTGATTAATCACCTGTATAAACGTGTCTTTTCCATGTTGATTTTTGTTTATGCAGATTGGGCTGAGGAAGGGCTCCCGTTCTTTTGCAGAAGCTCGTGCAGCTGGTTTCTCTGAAGAGAATGGGACTTTGGGTGATATCTATGAAACTGTTTCGGGAAGTGATCTTGTCTTGCTTCTGATTTCTGATTCTGCACAGGTCAGTTAGTCAAATTATCTGTACTCTGTTGCTTCTGGAACTATTAGGCTGGAATCAGAGTGTCTGCGCTGGAGTTTCTATAAACTGCTTGTGATAGTAAATATAGATGTATGCTTGGTTGCCTCTATCCTTCTGATATTGAGGACATAATTCTCCCTATATATGTGTGTATCCTTCAATGTAAACTTTCAATTATGGGCAAACTGATGACAACTTTTTCATGGTATATGCATTATATTACCTACTTCTTTCCCTTATGCTTGCCTTCTTTCATGTTTTTGATAAACTGTGAAGTTCTTAATGTTGTTTTACTTTGCAGGCTGATAATTATGAAAAAATATTCTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGGCATTTGCAGTCATTGGGGCTTGATTTCCCTAAGAATTTTAGCGTGATTGCTGTTTGTCCAAAGGGGATGGGTCCATCTGTGAGGAGACTTTACGTTCAAGGAAAAGAGATAAATGGTGCAGGAATTAATTCTAGTTTTGCAGTTCACCAGGTAATCTCGCATTCTTCACTTTAAGGTGGATATTTATCAATATACTTGGTTGTTAATTACATACAGTGTACAGGATGTTGATGGTAGAGCCACAGATGTTGCGTTGGGATGGTCTGTTGCCCTTGGCTCTCCATTCACATTTGCTACGTCTCTAGAGCAAGAATACAAGAGTGACATCTTTGGCGAGCGAGGTAAACTCACTCTACTTCTTGACTTCTTGTACCAGTTAAATCTGCATAAATTCTGAATACCCAACTTCTGCTGCTGTGGTCAACAGGCATTCTACTTGGTGCTGTTCATGGAATTGTGGAGTCCTTGTTTAGAAGGTACACTGAAAATGGAATGGCTGAAGAGCTGGCTTATAAGAACACTGTTGAATCCATAACAGGGATTATATCAAAGACCATTTCCACTAAGGTTGTATCTGCTGGCTAGATTTAACTGCAGTAACATTCACACTTGCTTCTTTATTAATGGCTGTTTGTTTTTTATGTGTAGGGAATGTTGGCGGTGTACAATTCTTTCTCTCCTGATGAGAAAAAGGAATTTGAGAAAGCATATAGTGCATCATATTATCCCTGTATGGATATTCTCTACGAATGTTATGAAGATGTAGCTAGTGGAAGTGAGATTCGCAGTGTTGTGCTTGCTGGACGGCGATTTTACGTATGTTGACATTCCTTTTTTTTTTTTCATCGCAGCAATAATATCTGATGAAATCAATAGTTTGTTGTTTAAACGAGCTATTTTACATTGATCTTTTTAGTTCTAGTTGGATAATTGTCGTACTGCTTGTTATACTTCACAACCTTATCTGCTTTTTGTTGGGTTTTTTTTCACATTATAGGAAAAGGAAGGTTTACCAGCTTTCCCAATGGGGAAAATTGACCAAACCCGAATGTGGAAGGTCGGTGAACGTGTCCGCCAAGTACGTCCTGCAGGTGACCTTGGCCCGCTACATCCTTTCACTGCTGGTGTATATGTGGCATTGATGATGGCGCAGGTATGAGATTTCCCTCCTAAACCTTAAACTTTATTCCAGTGCAACTTAAGTGTTCATCAAAATAAAGCAGCTGTCTAACGCATACTTCATGTCAAACTTCTATTTCCAGATTGAGATACTGAGGAAAAAGGGACACTCTTACTCTGAAATCATCAATGAAAGCGTGATCGAATCAGTGGACTCTCTGAACCCATTCATGCATGCTCGTGGGGTTTCATTTATGGTCGACAACTGCTCCACAACCGCACGATTGGGATCACGAAAATGGGCTCCACGTTTTGATTACATTGTCACCCAACAAGCCCTGGTCGCCATCGACAACGGTTCAGCTGTTAACCAGGACCTCATCAGCAACTTCCTGTCTGACCCAGTTCATGGAGCAATTGAAACATGCGCCCAGTTGAGACCTACCGTCGACATTTCTGTTCCACCTGATGCCGATTTTGTTCGCCCAGAGCTTCGTCAGTCTGGCAACTAG

mRNA sequence

ATGGCGGCCGCCACATCTTTCTCCCCCGCTATCTCAGCCTCATCATCCAAAACCCTAAAGAAGGCTGCTCTCAAGCCCCCGGCTCCGGCCAGCTTAGGGTTCTTGTCGTCATCTTCTTCCATGGGATCGAAGGCTCTCAAGGCTGTTGGCGTCAGTGGTGCTAATGCCGACGGCTCGGCTCTTGGTGCTCGCATGGTGTCCGTGCCTGCGATTAAGCCCTTAACTTCGCTCGATTTTGACACATCTGTCTTCAAGAAGGAGAAAGTCAATCTCGCCGGCCACGACGAGTATATTGTGAGAGGAGGTAGGGATCTTTTTCCCTTGCTGCCGGAAGCATTCAAGGGGATCAAGCAAATTGGTGTCATTGGCTGGGGTTCTCAGGGACCTGCACAGGCTCAGAATTTGAGGGACTCGCTTGCTGAAGCTAAGTCTGATATTGTGGTTAAGATTGGGCTGAGGAAGGGCTCCCGTTCTTTTGCAGAAGCTCGTGCAGCTGGTTTCTCTGAAGAGAATGGGACTTTGGGTGATATCTATGAAACTGTTTCGGGAAGTGATCTTGTCTTGCTTCTGATTTCTGATTCTGCACAGGCTGATAATTATGAAAAAATATTCTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGGCATTTGCAGTCATTGGGGCTTGATTTCCCTAAGAATTTTAGCGTGATTGCTGTTTGTCCAAAGGGGATGGGTCCATCTGTGAGGAGACTTTACGTTCAAGGAAAAGAGATAAATGGTGCAGGAATTAATTCTAGTTTTGCAGTTCACCAGGATGTTGATGGTAGAGCCACAGATGTTGCGTTGGGATGGTCTGTTGCCCTTGGCTCTCCATTCACATTTGCTACGTCTCTAGAGCAAGAATACAAGAGTGACATCTTTGGCGAGCGAGGCATTCTACTTGGTGCTGTTCATGGAATTGTGGAGTCCTTGTTTAGAAGGTACACTGAAAATGGAATGGCTGAAGAGCTGGCTTATAAGAACACTGTTGAATCCATAACAGGGATTATATCAAAGACCATTTCCACTAAGGGAATGTTGGCGGTGTACAATTCTTTCTCTCCTGATGAGAAAAAGGAATTTGAGAAAGCATATAGTGCATCATATTATCCCTGTATGGATATTCTCTACGAATGTTATGAAGATGTAGCTAGTGGAAGTGAGATTCGCAGTGTTGTGCTTGCTGGACGGCGATTTTACGAAAAGGAAGGTTTACCAGCTTTCCCAATGGGGAAAATTGACCAAACCCGAATGTGGAAGGTCGGTGAACGTGTCCGCCAAGTACGTCCTGCAGGTGACCTTGGCCCGCTACATCCTTTCACTGCTGGTGTATATGTGGCATTGATGATGGCGCAGATTGAGATACTGAGGAAAAAGGGACACTCTTACTCTGAAATCATCAATGAAAGCGTGATCGAATCAGTGGACTCTCTGAACCCATTCATGCATGCTCGTGGGGTTTCATTTATGGTCGACAACTGCTCCACAACCGCACGATTGGGATCACGAAAATGGGCTCCACGTTTTGATTACATTGTCACCCAACAAGCCCTGGTCGCCATCGACAACGGTTCAGCTGTTAACCAGGACCTCATCAGCAACTTCCTGTCTGACCCAGTTCATGGAGCAATTGAAACATGCGCCCAGTTGAGACCTACCGTCGACATTTCTGTTCCACCTGATGCCGATTTTGTTCGCCCAGAGCTTCGTCAGTCTGGCAACTAG

Coding sequence (CDS)

ATGGCGGCCGCCACATCTTTCTCCCCCGCTATCTCAGCCTCATCATCCAAAACCCTAAAGAAGGCTGCTCTCAAGCCCCCGGCTCCGGCCAGCTTAGGGTTCTTGTCGTCATCTTCTTCCATGGGATCGAAGGCTCTCAAGGCTGTTGGCGTCAGTGGTGCTAATGCCGACGGCTCGGCTCTTGGTGCTCGCATGGTGTCCGTGCCTGCGATTAAGCCCTTAACTTCGCTCGATTTTGACACATCTGTCTTCAAGAAGGAGAAAGTCAATCTCGCCGGCCACGACGAGTATATTGTGAGAGGAGGTAGGGATCTTTTTCCCTTGCTGCCGGAAGCATTCAAGGGGATCAAGCAAATTGGTGTCATTGGCTGGGGTTCTCAGGGACCTGCACAGGCTCAGAATTTGAGGGACTCGCTTGCTGAAGCTAAGTCTGATATTGTGGTTAAGATTGGGCTGAGGAAGGGCTCCCGTTCTTTTGCAGAAGCTCGTGCAGCTGGTTTCTCTGAAGAGAATGGGACTTTGGGTGATATCTATGAAACTGTTTCGGGAAGTGATCTTGTCTTGCTTCTGATTTCTGATTCTGCACAGGCTGATAATTATGAAAAAATATTCTCTCACATGAAACCAAACAGCATTCTTGGGCTTTCCCATGGGTTTCTTCTTGGGCATTTGCAGTCATTGGGGCTTGATTTCCCTAAGAATTTTAGCGTGATTGCTGTTTGTCCAAAGGGGATGGGTCCATCTGTGAGGAGACTTTACGTTCAAGGAAAAGAGATAAATGGTGCAGGAATTAATTCTAGTTTTGCAGTTCACCAGGATGTTGATGGTAGAGCCACAGATGTTGCGTTGGGATGGTCTGTTGCCCTTGGCTCTCCATTCACATTTGCTACGTCTCTAGAGCAAGAATACAAGAGTGACATCTTTGGCGAGCGAGGCATTCTACTTGGTGCTGTTCATGGAATTGTGGAGTCCTTGTTTAGAAGGTACACTGAAAATGGAATGGCTGAAGAGCTGGCTTATAAGAACACTGTTGAATCCATAACAGGGATTATATCAAAGACCATTTCCACTAAGGGAATGTTGGCGGTGTACAATTCTTTCTCTCCTGATGAGAAAAAGGAATTTGAGAAAGCATATAGTGCATCATATTATCCCTGTATGGATATTCTCTACGAATGTTATGAAGATGTAGCTAGTGGAAGTGAGATTCGCAGTGTTGTGCTTGCTGGACGGCGATTTTACGAAAAGGAAGGTTTACCAGCTTTCCCAATGGGGAAAATTGACCAAACCCGAATGTGGAAGGTCGGTGAACGTGTCCGCCAAGTACGTCCTGCAGGTGACCTTGGCCCGCTACATCCTTTCACTGCTGGTGTATATGTGGCATTGATGATGGCGCAGATTGAGATACTGAGGAAAAAGGGACACTCTTACTCTGAAATCATCAATGAAAGCGTGATCGAATCAGTGGACTCTCTGAACCCATTCATGCATGCTCGTGGGGTTTCATTTATGGTCGACAACTGCTCCACAACCGCACGATTGGGATCACGAAAATGGGCTCCACGTTTTGATTACATTGTCACCCAACAAGCCCTGGTCGCCATCGACAACGGTTCAGCTGTTAACCAGGACCTCATCAGCAACTTCCTGTCTGACCCAGTTCATGGAGCAATTGAAACATGCGCCCAGTTGAGACCTACCGTCGACATTTCTGTTCCACCTGATGCCGATTTTGTTCGCCCAGAGCTTCGTCAGTCTGGCAACTAG

Protein sequence

MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN
Homology
BLAST of Moc04g01100 vs. NCBI nr
Match: XP_022147921.1 (ketol-acid reductoisomerase, chloroplastic [Momordica charantia])

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA 60
           MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA
Sbjct: 1   MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA 60

Query: 61  LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG 120
           LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG
Sbjct: 61  LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG 120

Query: 121 VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET 180
           VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET
Sbjct: 121 VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET 180

Query: 181 VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV 240
           VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV
Sbjct: 181 VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV 240

Query: 241 CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE 300
           CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE
Sbjct: 241 CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE 300

Query: 301 QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM 360
           QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM
Sbjct: 301 QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM 360

Query: 361 LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP 420
           LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP
Sbjct: 361 LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP 420

Query: 421 AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI 480
           AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI
Sbjct: 421 AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI 480

Query: 481 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA 540
           INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA
Sbjct: 481 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA 540

Query: 541 VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN
Sbjct: 541 VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 588

BLAST of Moc04g01100 vs. NCBI nr
Match: XP_038888114.1 (ketol-acid reductoisomerase, chloroplastic [Benincasa hispida])

HSP 1 Score: 1085.1 bits (2805), Expect = 0.0e+00
Identity = 551/589 (93.55%), Postives = 570/589 (96.77%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKK-AALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGS 60
           MAAAT F+P+IS SS+KTLK+ AAL+P  P+SLGFLSSSSS GS ALKAVG+ GAN  GS
Sbjct: 1   MAAATYFAPSISVSSTKTLKQAAALQPLTPSSLGFLSSSSSSGSNALKAVGMGGANGVGS 60

Query: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQI 120
           ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+I
Sbjct: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI 120

Query: 121 GVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYE 180
           GVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIYE
Sbjct: 121 GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIYE 180

Query: 181 TVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIA 240
           T+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVIA
Sbjct: 181 TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVIA 240

Query: 241 VCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSL 300
           VCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT+L
Sbjct: 241 VCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATTL 300

Query: 301 EQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKG 360
           EQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIISKTISTKG
Sbjct: 301 EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTKG 360

Query: 361 MLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420
           MLAVYNSFSPDEKKEFE+AYSASYYPCMDILYECYEDVA+GSEIRSVVLAGRRFYEKEGL
Sbjct: 361 MLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVATGSEIRSVVLAGRRFYEKEGL 420

Query: 421 PAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSE 480
           PAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYSE
Sbjct: 421 PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE 480

Query: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGS 540
           IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNGS
Sbjct: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS 540

Query: 541 AVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+NQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSNN 589

BLAST of Moc04g01100 vs. NCBI nr
Match: XP_008455269.1 (PREDICTED: ketol-acid reductoisomerase, chloroplastic [Cucumis melo] >KAA0031534.1 ketol-acid reductoisomerase [Cucumis melo var. makuwa] >TYK06987.1 ketol-acid reductoisomerase [Cucumis melo var. makuwa])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 551/589 (93.55%), Postives = 571/589 (96.94%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKK-AALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGS 60
           MAAAT F+P+ISASS+KTLK+ AAL+P +P+SLGFLSSSSS G  ALKAVG+ GAN  GS
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFLSSSSS-GLYALKAVGICGANGVGS 60

Query: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQI 120
           ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+I
Sbjct: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI 120

Query: 121 GVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYE 180
           GVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIYE
Sbjct: 121 GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIYE 180

Query: 181 TVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIA 240
           T+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVIA
Sbjct: 181 TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVIA 240

Query: 241 VCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSL 300
           VCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTFAT+L
Sbjct: 241 VCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTFATTL 300

Query: 301 EQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKG 360
           EQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIIS+TISTKG
Sbjct: 301 EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISRTISTKG 360

Query: 361 MLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420
           MLAVYNSFSPDEKKEFE+AYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL
Sbjct: 361 MLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420

Query: 421 PAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSE 480
           PAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYSE
Sbjct: 421 PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE 480

Query: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGS 540
           IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNGS
Sbjct: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS 540

Query: 541 AVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+NQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of Moc04g01100 vs. NCBI nr
Match: XP_022952496.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 555/590 (94.07%), Postives = 568/590 (96.27%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAA-LKPPAPASLGFLSSSS-SMGSKALKAVGVSGANADG 60
           MAAAT FSP+ISAS SKTLK+AA L P  P+SLGFLSSSS S GS ALKAVG+ G NA G
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIY 180
           IGVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ET+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATS 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT+
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTK 360
           LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFE+AYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           SAVNQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of Moc04g01100 vs. NCBI nr
Match: XP_023554080.1 (ketol-acid reductoisomerase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 554/590 (93.90%), Postives = 568/590 (96.27%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAA-LKPPAPASLGFLSSSS-SMGSKALKAVGVSGANADG 60
           MAAAT FSP+ISAS SKTLK+AA L P  P+SLGFLSSSS S GS ALKAVG+ G NA G
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIY 180
           IGVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ET+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATS 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT+
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTK 360
           LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFE+AYSAS+YPCMDILYECYEDVA+GSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVATGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           SAVNQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of Moc04g01100 vs. ExPASy Swiss-Prot
Match: Q01292 (Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=AHRI PE=1 SV=1)

HSP 1 Score: 973.8 bits (2516), Expect = 8.8e-283
Identity = 496/591 (83.93%), Postives = 541/591 (91.54%), Query Frame = 0

Query: 3   AATSFSPAISASSSKTLKKAALKPPAPA--SLGFLSSSSSM-GSKALKAVGV--SGANAD 62
           AAT+FS + S+S+S    KA  + P P+  +LGFL SSS++   ++LKA  V  SGAN  
Sbjct: 5   AATTFSLSSSSSTSAAASKALKQSPKPSALNLGFLGSSSTIKACRSLKAARVLPSGANGG 64

Query: 63  GSALGARMVSVPAIK--PLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKG 122
           GSAL A+MVS P+I     T+ DFD+SVFKKEKV L+GHDEYIVRGGR+LFPLLP+AFKG
Sbjct: 65  GSALSAQMVSAPSINTPSATTFDFDSSVFKKEKVTLSGHDEYIVRGGRNLFPLLPDAFKG 124

Query: 123 IKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLG 182
           IKQIGVIGWGSQ PAQAQNL+DSL EAKSD+VVKIGLRKGS SFAEARAAGFSEENGTLG
Sbjct: 125 IKQIGVIGWGSQAPAQAQNLKDSLTEAKSDVVVKIGLRKGSNSFAEARAAGFSEENGTLG 184

Query: 183 DIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNF 242
           D++ET+SGSDLVLLLISDSAQADNYEK+FSHMKPNSILGLSHGFLLGHLQSLG DFPKN 
Sbjct: 185 DMWETISGSDLVLLLISDSAQADNYEKVFSHMKPNSILGLSHGFLLGHLQSLGQDFPKNI 244

Query: 243 SVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTF 302
           SVIAVCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTF
Sbjct: 245 SVIAVCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTF 304

Query: 303 ATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTI 362
           AT+LEQEYKSDIFGERGILLGAVHGIVE LFRRYTE+GM+E+LAYKNTVE ITG+ISKTI
Sbjct: 305 ATTLEQEYKSDIFGERGILLGAVHGIVECLFRRYTESGMSEDLAYKNTVECITGVISKTI 364

Query: 363 STKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYE 422
           STKGMLA+YNS S + KK+F+ AYSASYYP MDILYECYEDVASGSEIRSVVLAGRRFYE
Sbjct: 365 STKGMLALYNSLSEEGKKDFQAAYSASYYPSMDILYECYEDVASGSEIRSVVLAGRRFYE 424

Query: 423 KEGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGH 482
           KEGLPAFPMGKIDQTRMWKVGE+VR VRPAGDLGPL+PFTAGVYVALMMAQIEILRKKGH
Sbjct: 425 KEGLPAFPMGKIDQTRMWKVGEKVRSVRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGH 484

Query: 483 SYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAI 542
           SYSEIINESVIE+VDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI++QQALVA+
Sbjct: 485 SYSEIINESVIEAVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILSQQALVAV 544

Query: 543 DNGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQS 587
           DNG+ +NQDLISNFLSDPVH AI  CAQLRP+VDISV  DADFVRPELRQ+
Sbjct: 545 DNGAPINQDLISNFLSDPVHEAIGVCAQLRPSVDISVTADADFVRPELRQA 595

BLAST of Moc04g01100 vs. ExPASy Swiss-Prot
Match: Q05758 (Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At3g58610 PE=1 SV=2)

HSP 1 Score: 973.0 bits (2514), Expect = 1.5e-282
Identity = 499/595 (83.87%), Postives = 545/595 (91.60%), Query Frame = 0

Query: 1   MAAAT-SFSPAIS----ASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAV--GVSG 60
           MAAAT S +P++S    +SSSKTL  +  +  A  ++GFLSSS    SK+L+++   V+G
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKARTLALPNIGFLSSS----SKSLRSLTATVAG 60

Query: 61  ANADGSALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS  A+K   SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  NGATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGT 180
           KGIKQIGVIGWGSQGPAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARAAGF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ET++GSDLVLLLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISK 360
           TFAT+LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMG IDQTRMWKVGERVR+ RPAGDLGPL+PFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+D+G+A+N+DLISNF SDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

BLAST of Moc04g01100 vs. ExPASy Swiss-Prot
Match: O82043 (Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum OX=3888 GN=PGAAIR PE=2 SV=1)

HSP 1 Score: 969.5 bits (2505), Expect = 1.7e-281
Identity = 492/588 (83.67%), Postives = 530/588 (90.14%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA 60
           MAA TS      ++SSKTL K      AP +L F S  S    +A +++ V      GSA
Sbjct: 1   MAAVTSSCSTAISASSKTLAKPVAASFAPTNLSF-SKLSPQSIRARRSITV------GSA 60

Query: 61  LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG 120
           LGA  VS P      SLDF+TSVFKKE+VNLAGH+EYIVRGGRDLF LLP+AFKGIKQIG
Sbjct: 61  LGATKVSAPPATHPVSLDFETSVFKKERVNLAGHEEYIVRGGRDLFHLLPDAFKGIKQIG 120

Query: 121 VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET 180
           VIGWGSQGPAQAQNLRDSL EAKSDIVVK+GLRKGS SF EAR AGFSEE GTLGDI+ET
Sbjct: 121 VIGWGSQGPAQAQNLRDSLVEAKSDIVVKVGLRKGSSSFNEAREAGFSEEKGTLGDIWET 180

Query: 181 VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV 240
           +SGSDLVLLLISDSAQADNYEKIFSH+KPNSILGLSHGFLLGHLQS+GLDFPKNFSVIAV
Sbjct: 181 ISGSDLVLLLISDSAQADNYEKIFSHLKPNSILGLSHGFLLGHLQSIGLDFPKNFSVIAV 240

Query: 241 CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE 300
           CPKGMGPSVRRLYVQGKEINGAGINSSF VHQDVDGRAT+VALGWSVALGSPFTFAT+LE
Sbjct: 241 CPKGMGPSVRRLYVQGKEINGAGINSSFGVHQDVDGRATNVALGWSVALGSPFTFATTLE 300

Query: 301 QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM 360
           QEYKSDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVESITG+ISKTIST+GM
Sbjct: 301 QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVESITGVISKTISTQGM 360

Query: 361 LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP 420
           LAVYN+ S D KKEFEKAYSAS+YPCM+ILYECYEDVASGSEIRSVVLAGRRFYEKEGLP
Sbjct: 361 LAVYNALSEDGKKEFEKAYSASFYPCMEILYECYEDVASGSEIRSVVLAGRRFYEKEGLP 420

Query: 421 AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI 480
           AFPMGKIDQTRMWKVGERVR  RPAGDLGPL+PFTAGV+VA+MMAQIE+LRKKGHSYSEI
Sbjct: 421 AFPMGKIDQTRMWKVGERVRSTRPAGDLGPLYPFTAGVFVAMMMAQIEVLRKKGHSYSEI 480

Query: 481 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA 540
           INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVA+D+G+ 
Sbjct: 481 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAVDSGAP 540

Query: 541 VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           +NQDLISNF+SDPVHGAI+ CA+LRPT+DISVP  ADFVRPELRQ  N
Sbjct: 541 INQDLISNFVSDPVHGAIQVCAELRPTLDISVPAAADFVRPELRQCSN 581

BLAST of Moc04g01100 vs. ExPASy Swiss-Prot
Match: Q65XK0 (Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0573700 PE=1 SV=1)

HSP 1 Score: 943.7 bits (2438), Expect = 9.8e-274
Identity = 485/590 (82.20%), Postives = 520/590 (88.14%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAAL----KPPAPASLGFLSSSSSMGSKALKAVGVSGANA 60
           MAA+T+    ++ S  KTL  AA      P APA++ F  S ++    A +   V+   A
Sbjct: 1   MAASTT----LALSHPKTLAAAAAAAPKAPTAPAAVSFPVSHAACAPLAARRRAVTAMVA 60

Query: 61  DGSALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGI 120
              A+GA M          SLDFDTSVF KEKV+LAGH+EYIVRGGR+LFPLLPEAFKGI
Sbjct: 61  APPAVGAAM---------PSLDFDTSVFNKEKVSLAGHEEYIVRGGRNLFPLLPEAFKGI 120

Query: 121 KQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGD 180
           KQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGS+SF EARAAGF+EE+GTLGD
Sbjct: 121 KQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSKSFDEARAAGFTEESGTLGD 180

Query: 181 IYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFS 240
           I+ETVSGSDLVLLLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPKN S
Sbjct: 181 IWETVSGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSAGLDFPKNIS 240

Query: 241 VIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFA 300
           VIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFA
Sbjct: 241 VIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFA 300

Query: 301 TSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTIS 360
           T+LEQEYKSDIFGERGILLGAVHGIVE+LFRRYTE GM EE+AYKNTVE ITGIISKTIS
Sbjct: 301 TTLEQEYKSDIFGERGILLGAVHGIVEALFRRYTEQGMDEEMAYKNTVEGITGIISKTIS 360

Query: 361 TKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEK 420
            KGML VYNS + + KKEF KAYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEK
Sbjct: 361 KKGMLEVYNSLTEEGKKEFNKAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEK 420

Query: 421 EGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHS 480
           EGLPAFPMG IDQTRMWKVGE+VR  RP  DLGPLHPFTAGVYVALMMAQIE+LRKKGHS
Sbjct: 421 EGLPAFPMGNIDQTRMWKVGEKVRSTRPENDLGPLHPFTAGVYVALMMAQIEVLRKKGHS 480

Query: 481 YSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAID 540
           YSEIINESVIESVDSLNPFMHARGV+FMVDNCSTTARLGSRKWAPRFDYI+TQQA V +D
Sbjct: 481 YSEIINESVIESVDSLNPFMHARGVAFMVDNCSTTARLGSRKWAPRFDYILTQQAFVTVD 540

Query: 541 NGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQS 587
             + +NQDLISNF+SDPVHGAIE CA+LRPTVDISVP +ADFVRPELRQS
Sbjct: 541 KDAPINQDLISNFMSDPVHGAIEVCAELRPTVDISVPANADFVRPELRQS 577

BLAST of Moc04g01100 vs. ExPASy Swiss-Prot
Match: P38674 (Ketol-acid reductoisomerase, mitochondrial OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=ilv-2 PE=3 SV=2)

HSP 1 Score: 164.1 bits (414), Expect = 4.8e-39
Identity = 118/400 (29.50%), Postives = 202/400 (50.50%), Query Frame = 0

Query: 51  VSGANADGSALGARMVSVPA---IKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFP 110
           V+ A+A  +++  + V+ PA   ++ + ++DF            AGH E +         
Sbjct: 28  VAAASAVRASVAVKAVAAPARQQVRGVKTMDF------------AGHKEEVHERADWPAE 87

Query: 111 LLPEAFKGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGF 170
            L + FK    + +IG+GSQG  Q  NLRD      + + V +G+RK  +S+ +A   G+
Sbjct: 88  KLLDYFKN-DTLALIGYGSQGHGQGLNLRD------NGLNVIVGVRKNGKSWEDAIQDGW 147

Query: 171 SEENGTLGDIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSL 230
                 L D+ E +S   +V+ L+SD+AQ++ +  I   +     L  SHGF        
Sbjct: 148 -VPGKNLFDVDEAISRGTIVMNLLSDAAQSETWPHIKPQITKGKTLYFSHGFSPVFKDLT 207

Query: 231 GLDFPKNFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSV 290
            ++ P +  VI V PKG G +VR L+ +G+     GINSSFAV+QDV G+A + A+   V
Sbjct: 208 KVEVPTDVDVILVAPKGSGRTVRSLFREGR-----GINSSFAVYQDVTGKAKEKAVALGV 267

Query: 291 ALGSPFTFATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESI 350
           A+GS + + T+ E+E  SD++GERG L+G +HG+  + +    E G +   A+  TVE  
Sbjct: 268 AVGSGYLYETTFEKEVYSDLYGERGCLMGGIHGMFLAQYEVLRERGHSPSEAFNETVEEA 327

Query: 351 TGIISKTISTKGMLAVYNSFSPDEKK---EFEKAYSASYYPCMDILYECYEDVASGSEIR 410
           T  +   I   GM  ++++ S   ++   ++   +  +  P  + L   Y+ V +G E +
Sbjct: 328 TQSLYPLIGAHGMDWMFDACSTTARRGAIDWTPKFKDALKPVFNNL---YDSVKNGDETK 387

Query: 411 SVVLAGRRFYEKEGLPAFPMGKIDQTRMWKVGERVRQVRP 445
             +    +   +E   A  + +I    +W+ G+ VR +RP
Sbjct: 388 RSLEYNSQPDYRERYEA-ELDEIRNLEIWRAGKAVRSLRP 398

BLAST of Moc04g01100 vs. ExPASy TrEMBL
Match: A0A6J1D3T7 (Ketol-acid reductoisomerase OS=Momordica charantia OX=3673 GN=LOC111016733 PE=3 SV=1)

HSP 1 Score: 1152.1 bits (2979), Expect = 0.0e+00
Identity = 588/588 (100.00%), Postives = 588/588 (100.00%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA 60
           MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA
Sbjct: 1   MAAATSFSPAISASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGSA 60

Query: 61  LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG 120
           LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG
Sbjct: 61  LGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQIG 120

Query: 121 VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET 180
           VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET
Sbjct: 121 VIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYET 180

Query: 181 VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV 240
           VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV
Sbjct: 181 VSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIAV 240

Query: 241 CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE 300
           CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE
Sbjct: 241 CPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSLE 300

Query: 301 QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM 360
           QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM
Sbjct: 301 QEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKGM 360

Query: 361 LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP 420
           LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP
Sbjct: 361 LAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGLP 420

Query: 421 AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI 480
           AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI
Sbjct: 421 AFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSEI 480

Query: 481 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA 540
           INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA
Sbjct: 481 INESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGSA 540

Query: 541 VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN
Sbjct: 541 VNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 588

BLAST of Moc04g01100 vs. ExPASy TrEMBL
Match: A0A5D3C563 (Ketol-acid reductoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G003030 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 551/589 (93.55%), Postives = 571/589 (96.94%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKK-AALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGS 60
           MAAAT F+P+ISASS+KTLK+ AAL+P +P+SLGFLSSSSS G  ALKAVG+ GAN  GS
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFLSSSSS-GLYALKAVGICGANGVGS 60

Query: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQI 120
           ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+I
Sbjct: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI 120

Query: 121 GVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYE 180
           GVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIYE
Sbjct: 121 GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIYE 180

Query: 181 TVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIA 240
           T+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVIA
Sbjct: 181 TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVIA 240

Query: 241 VCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSL 300
           VCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTFAT+L
Sbjct: 241 VCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTFATTL 300

Query: 301 EQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKG 360
           EQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIIS+TISTKG
Sbjct: 301 EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISRTISTKG 360

Query: 361 MLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420
           MLAVYNSFSPDEKKEFE+AYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL
Sbjct: 361 MLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420

Query: 421 PAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSE 480
           PAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYSE
Sbjct: 421 PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE 480

Query: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGS 540
           IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNGS
Sbjct: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS 540

Query: 541 AVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+NQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of Moc04g01100 vs. ExPASy TrEMBL
Match: A0A1S3C039 (Ketol-acid reductoisomerase OS=Cucumis melo OX=3656 GN=LOC103495472 PE=3 SV=1)

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 551/589 (93.55%), Postives = 571/589 (96.94%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKK-AALKPPAPASLGFLSSSSSMGSKALKAVGVSGANADGS 60
           MAAAT F+P+ISASS+KTLK+ AAL+P +P+SLGFLSSSSS G  ALKAVG+ GAN  GS
Sbjct: 1   MAAATHFAPSISASSTKTLKQAAALQPLSPSSLGFLSSSSS-GLYALKAVGICGANGVGS 60

Query: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQI 120
           ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+I
Sbjct: 61  ALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKKI 120

Query: 121 GVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIYE 180
           GVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIYE
Sbjct: 121 GVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIYE 180

Query: 181 TVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVIA 240
           T+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKN SVIA
Sbjct: 181 TISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNISVIA 240

Query: 241 VCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATSL 300
           VCPKGMGPSVRRLYVQGKE+NGAGINSSFAVHQDVDGRATDVALGWS+ALGSPFTFAT+L
Sbjct: 241 VCPKGMGPSVRRLYVQGKEVNGAGINSSFAVHQDVDGRATDVALGWSIALGSPFTFATTL 300

Query: 301 EQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTKG 360
           EQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIIS+TISTKG
Sbjct: 301 EQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISRTISTKG 360

Query: 361 MLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420
           MLAVYNSFSPDEKKEFE+AYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL
Sbjct: 361 MLAVYNSFSPDEKKEFERAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEGL 420

Query: 421 PAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYSE 480
           PAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYSE
Sbjct: 421 PAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYSE 480

Query: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNGS 540
           IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNGS
Sbjct: 481 IINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNGS 540

Query: 541 AVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+NQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AINQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 588

BLAST of Moc04g01100 vs. ExPASy TrEMBL
Match: A0A6J1GKD7 (Ketol-acid reductoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111455167 PE=3 SV=1)

HSP 1 Score: 1080.5 bits (2793), Expect = 0.0e+00
Identity = 555/590 (94.07%), Postives = 568/590 (96.27%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAA-LKPPAPASLGFLSSSS-SMGSKALKAVGVSGANADG 60
           MAAAT FSP+ISAS SKTLK+AA L P  P+SLGFLSSSS S GS ALKAVG+ G NA G
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGSFALKAVGMGGGNAVG 60

Query: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120

Query: 121 IGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIY 180
           IGVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARA GFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAVGFSEENGTLGDIY 180

Query: 181 ETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ET+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATS 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT+
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTK 360
           LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFE+AYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           SAVNQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of Moc04g01100 vs. ExPASy TrEMBL
Match: A0A6J1HV87 (Ketol-acid reductoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111468154 PE=3 SV=1)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 553/590 (93.73%), Postives = 567/590 (96.10%), Query Frame = 0

Query: 1   MAAATSFSPAISASSSKTLKKAA-LKPPAPASLGFLSSSS-SMGSKALKAVGVSGANADG 60
           MAAAT FSP+ISAS SKTLK+AA L P  P+SLGFLSSSS S G  ALKAVG+ GANA G
Sbjct: 1   MAAATYFSPSISASLSKTLKQAASLHPLTPSSLGFLSSSSASSGFFALKAVGMGGANAVG 60

Query: 61  SALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKQ 120
           SALGARMVS PAIKPL SLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIK+
Sbjct: 61  SALGARMVSAPAIKPLISLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAFKGIKK 120

Query: 121 IGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGTLGDIY 180
           IGVIGWGSQ PAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEAR  GFSEENGTLGDIY
Sbjct: 121 IGVIGWGSQAPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARTVGFSEENGTLGDIY 180

Query: 181 ETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240
           ET+S SDLV+LLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI
Sbjct: 181 ETISESDLVMLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPKNFSVI 240

Query: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATS 300
           AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFAT+
Sbjct: 241 AVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPFTFATT 300

Query: 301 LEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISKTISTK 360
           LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGMAE+LAYKNTVESITGIISKTISTK
Sbjct: 301 LEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMAEDLAYKNTVESITGIISKTISTK 360

Query: 361 GMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420
           GMLAVYNSFSPDEKKEFE+AYSAS+YPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG
Sbjct: 361 GMLAVYNSFSPDEKKEFERAYSASFYPCMDILYECYEDVASGSEIRSVVLAGRRFYEKEG 420

Query: 421 LPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKKGHSYS 480
           LPAFPMG IDQTRMWKVGERVRQ RPAGDLGPL+PFTAGVYVALMMAQIEILRKKGHSYS
Sbjct: 421 LPAFPMGNIDQTRMWKVGERVRQSRPAGDLGPLYPFTAGVYVALMMAQIEILRKKGHSYS 480

Query: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALVAIDNG 540
           EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALVAIDNG
Sbjct: 481 EIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALVAIDNG 540

Query: 541 SAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           SAVNQDLISNFLSDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 SAVNQDLISNFLSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 590

BLAST of Moc04g01100 vs. TAIR 10
Match: AT3G58610.1 (ketol-acid reductoisomerase )

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-283
Identity = 499/595 (83.87%), Postives = 545/595 (91.60%), Query Frame = 0

Query: 1   MAAAT-SFSPAIS----ASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAV--GVSG 60
           MAAAT S +P++S    +SSSKTL  +  +  A  ++GFLSSS    SK+L+++   V+G
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKARTLALPNIGFLSSS----SKSLRSLTATVAG 60

Query: 61  ANADGSALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS  A+K   SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  NGATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGT 180
           KGIKQIGVIGWGSQGPAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARAAGF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ET++GSDLVLLLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISK 360
           TFAT+LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMG IDQTRMWKVGERVR+ RPAGDLGPL+PFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+D+G+A+N+DLISNF SDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

BLAST of Moc04g01100 vs. TAIR 10
Match: AT3G58610.2 (ketol-acid reductoisomerase )

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-283
Identity = 499/595 (83.87%), Postives = 545/595 (91.60%), Query Frame = 0

Query: 1   MAAAT-SFSPAIS----ASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAV--GVSG 60
           MAAAT S +P++S    +SSSKTL  +  +  A  ++GFLSSS    SK+L+++   V+G
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKARTLALPNIGFLSSS----SKSLRSLTATVAG 60

Query: 61  ANADGSALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS  A+K   SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  NGATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGT 180
           KGIKQIGVIGWGSQGPAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARAAGF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ET++GSDLVLLLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISK 360
           TFAT+LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMG IDQTRMWKVGERVR+ RPAGDLGPL+PFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+D+G+A+N+DLISNF SDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

BLAST of Moc04g01100 vs. TAIR 10
Match: AT3G58610.3 (ketol-acid reductoisomerase )

HSP 1 Score: 973.0 bits (2514), Expect = 1.1e-283
Identity = 499/595 (83.87%), Postives = 545/595 (91.60%), Query Frame = 0

Query: 1   MAAAT-SFSPAIS----ASSSKTLKKAALKPPAPASLGFLSSSSSMGSKALKAV--GVSG 60
           MAAAT S +P++S    +SSSKTL  +  +  A  ++GFLSSS    SK+L+++   V+G
Sbjct: 1   MAAATSSIAPSLSCPSPSSSSKTLWSSKARTLALPNIGFLSSS----SKSLRSLTATVAG 60

Query: 61  ANADGSALGARMVSVPAIKPLTSLDFDTSVFKKEKVNLAGHDEYIVRGGRDLFPLLPEAF 120
             A GS+L ARMVS  A+K   SLDF+TSVFKKEKV+LAG++EYIVRGGRDLF  LP+AF
Sbjct: 61  NGATGSSLAARMVSSSAVKAPVSLDFETSVFKKEKVSLAGYEEYIVRGGRDLFKHLPDAF 120

Query: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLAEAKSDIVVKIGLRKGSRSFAEARAAGFSEENGT 180
           KGIKQIGVIGWGSQGPAQAQNLRDSL EAKSDIVVKIGLRKGSRSF EARAAGF+EE+GT
Sbjct: 121 KGIKQIGVIGWGSQGPAQAQNLRDSLVEAKSDIVVKIGLRKGSRSFEEARAAGFTEESGT 180

Query: 181 LGDIYETVSGSDLVLLLISDSAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSLGLDFPK 240
           LGDI+ET++GSDLVLLLISD+AQADNYEKIFSHMKPNSILGLSHGFLLGHLQS GLDFPK
Sbjct: 181 LGDIWETIAGSDLVLLLISDAAQADNYEKIFSHMKPNSILGLSHGFLLGHLQSSGLDFPK 240

Query: 241 NFSVIAVCPKGMGPSVRRLYVQGKEINGAGINSSFAVHQDVDGRATDVALGWSVALGSPF 300
           N SV+AVCPKGMGPSVRRLYVQGKEINGAGIN+SFAVHQDVDGRA DVALGWSVALGSPF
Sbjct: 241 NISVVAVCPKGMGPSVRRLYVQGKEINGAGINASFAVHQDVDGRAADVALGWSVALGSPF 300

Query: 301 TFATSLEQEYKSDIFGERGILLGAVHGIVESLFRRYTENGMAEELAYKNTVESITGIISK 360
           TFAT+LEQEY+SDIFGERGILLGAVHGIVESLFRRYTENGM+E+LAYKNTVE ITG IS+
Sbjct: 301 TFATTLEQEYRSDIFGERGILLGAVHGIVESLFRRYTENGMSEDLAYKNTVECITGTISR 360

Query: 361 TISTKGMLAVYNSFSPDEKKEFEKAYSASYYPCMDILYECYEDVASGSEIRSVVLAGRRF 420
           TIST+GMLAVYNS S + KK+FE AYSAS+YPCM+ILYECYEDV SGSEIRSVVLAGRRF
Sbjct: 361 TISTQGMLAVYNSLSEEGKKDFETAYSASFYPCMEILYECYEDVQSGSEIRSVVLAGRRF 420

Query: 421 YEKEGLPAFPMGKIDQTRMWKVGERVRQVRPAGDLGPLHPFTAGVYVALMMAQIEILRKK 480
           YEKEGLPAFPMG IDQTRMWKVGERVR+ RPAGDLGPL+PFTAGVYVALMMAQIEILRKK
Sbjct: 421 YEKEGLPAFPMGNIDQTRMWKVGERVRKSRPAGDLGPLYPFTAGVYVALMMAQIEILRKK 480

Query: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYIVTQQALV 540
           GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYI+TQQALV
Sbjct: 481 GHSYSEIINESVIESVDSLNPFMHARGVSFMVDNCSTTARLGSRKWAPRFDYILTQQALV 540

Query: 541 AIDNGSAVNQDLISNFLSDPVHGAIETCAQLRPTVDISVPPDADFVRPELRQSGN 589
           A+D+G+A+N+DLISNF SDPVHGAIE CAQLRPTVDISVP DADFVRPELRQS N
Sbjct: 541 AVDSGAAINRDLISNFFSDPVHGAIEVCAQLRPTVDISVPADADFVRPELRQSSN 591

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147921.10.0e+00100.00ketol-acid reductoisomerase, chloroplastic [Momordica charantia][more]
XP_038888114.10.0e+0093.55ketol-acid reductoisomerase, chloroplastic [Benincasa hispida][more]
XP_008455269.10.0e+0093.55PREDICTED: ketol-acid reductoisomerase, chloroplastic [Cucumis melo] >KAA0031534... [more]
XP_022952496.10.0e+0094.07ketol-acid reductoisomerase, chloroplastic-like [Cucurbita moschata][more]
XP_023554080.10.0e+0093.90ketol-acid reductoisomerase, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q012928.8e-28383.93Ketol-acid reductoisomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=AHRI ... [more]
Q057581.5e-28283.87Ketol-acid reductoisomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At... [more]
O820431.7e-28183.67Ketol-acid reductoisomerase, chloroplastic OS=Pisum sativum OX=3888 GN=PGAAIR PE... [more]
Q65XK09.8e-27482.20Ketol-acid reductoisomerase, chloroplastic OS=Oryza sativa subsp. japonica OX=39... [more]
P386744.8e-3929.50Ketol-acid reductoisomerase, mitochondrial OS=Neurospora crassa (strain ATCC 246... [more]
Match NameE-valueIdentityDescription
A0A6J1D3T70.0e+00100.00Ketol-acid reductoisomerase OS=Momordica charantia OX=3673 GN=LOC111016733 PE=3 ... [more]
A0A5D3C5630.0e+0093.55Ketol-acid reductoisomerase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
A0A1S3C0390.0e+0093.55Ketol-acid reductoisomerase OS=Cucumis melo OX=3656 GN=LOC103495472 PE=3 SV=1[more]
A0A6J1GKD70.0e+0094.07Ketol-acid reductoisomerase OS=Cucurbita moschata OX=3662 GN=LOC111455167 PE=3 S... [more]
A0A6J1HV870.0e+0093.73Ketol-acid reductoisomerase OS=Cucurbita maxima OX=3661 GN=LOC111468154 PE=3 SV=... [more]
Match NameE-valueIdentityDescription
AT3G58610.11.1e-28383.87ketol-acid reductoisomerase [more]
AT3G58610.21.1e-28383.87ketol-acid reductoisomerase [more]
AT3G58610.31.1e-28383.87ketol-acid reductoisomerase [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.720coord: 65..292
e-value: 4.0E-111
score: 371.7
NoneNo IPR availablePIRSRPIRSR000118-2PIRSR000118-2coord: 2..586
e-value: 0.0
score: 1233.2
NoneNo IPR availablePIRSRPIRSR000118-1PIRSR000118-1coord: 2..586
e-value: 0.0
score: 1233.2
NoneNo IPR availablePANTHERPTHR21371:SF20KETOL-ACID REDUCTOISOMERASEcoord: 3..587
IPR000506Ketol-acid reductoisomerase, C-terminalPFAMPF01450IlvCcoord: 457..528
e-value: 9.6E-8
score: 32.4
coord: 300..444
e-value: 6.7E-35
score: 120.5
IPR000506Ketol-acid reductoisomerase, C-terminalPROSITEPS51851KARI_Ccoord: 298..446
score: 34.245323
IPR000506Ketol-acid reductoisomerase, C-terminalPROSITEPS51851KARI_Ccoord: 447..583
score: 22.552483
IPR013116Ketol-acid reductoisomerase, N-terminalPFAMPF07991IlvNcoord: 117..291
e-value: 1.3E-30
score: 106.2
IPR013116Ketol-acid reductoisomerase, N-terminalPROSITEPS51850KARI_Ncoord: 99..297
score: 51.455605
IPR016206Ketol-acid reductoisomerase, plantPIRSFPIRSF000118Ilv5_plantcoord: 1..587
e-value: 0.0
score: 1227.6
IPR0133286-phosphogluconate dehydrogenase, domain 2GENE3D1.10.1040.10coord: 293..566
e-value: 4.8E-156
score: 520.3
IPR013023Ketol-acid reductoisomerasePANTHERPTHR21371KETOL-ACID REDUCTOISOMERASE, MITOCHONDRIALcoord: 3..587
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 77..295
IPR0089276-phosphogluconate dehydrogenase-like, C-terminal domain superfamilySUPERFAMILY481796-phosphogluconate dehydrogenase C-terminal domain-likecoord: 300..585

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g01100.1Moc04g01100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009097 isoleucine biosynthetic process
biological_process GO:0009099 valine biosynthetic process
biological_process GO:0009082 branched-chain amino acid biosynthetic process
molecular_function GO:0016853 isomerase activity
molecular_function GO:0004455 ketol-acid reductoisomerase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016491 oxidoreductase activity