Moc04g00250 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc04g00250
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Descriptionphosphoglycerate kinase
Locationchr4: 174857 .. 177076 (-)
RNA-Seq ExpressionMoc04g00250
SyntenyMoc04g00250
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTACCAAAAGGAGCGTTGGTACTCTGAAGGAGGCTGATTTGAAGGGAAAGAGGGTGTTCGTGAGGGTCGATCTCAATGTTCCTTTGGATGACAACTTCAACATCACCGATGACACCAGAATCCGTGCTGCTGTGCCTACCATTAAGTATTTACTCGGCCATGGTGCCAGGGTCATCCTCTCCAGCCACTTGGTGCGCTACTTCTCCTCTTTCCCTCTTTCGCATTCTCGCAAGATCTATCTATTTGGCATGTTCTCCCGAGTTCTACTTTGGGTTTGCTTCGATCTTTGCATTTTACAGCATGCCCGACAATCGTAACTTTCGATCCTTTGTTTTCAGGAAAGGCCATCATCCCTCTCCCCATTTTTATCATCTTTTTTGGTGGATTTAGATAAACTTGAAAAGGAATATTGCGATTTTGGATAATGTACAATTCGATTGTATTGTCATACGTCATATGCACGATTCAGCTTTTTGGATCTAATATTCATTATGCTCAATATTGGAAAATTCATTGACATGCAGTGTAAAGATCAACGTTGATTATTGTTAGTTAAATTTTCAGTGATCTGCGCCTTTCTTCTTCCGTAAACTTTGGAGTGACGCTGCGTATTTGATTGCGATCGATTAAGATTATGATCTTTACTGAACTGAGGTCGCTTATTGTGTTCAATTTCATGTTAGCATCACTGTTCGATTTGTTTTCAAATTTGCATTATTTTTCATTTTGTCTATTCCCTGAGCTCATTTTGTTTTTTATTATTGTGGTCTTTCAGGGGCGCCCAAAGGGCGTAACGCCGAAATACAGTTTGAAACCTCTTGTTCCCAGGCTTTCTGAGCTCCTCGGAGTTCAGGTATGGCGCCTAAATTTCGTAGAAATATTTGTTTGGGATTTGTGTTTATATGTGATGACAACGTTATCCCTTACTAAAATTTTGTCCCCTCCCCCCACAATAGGTGAAAATGGCCAATGATTCTATTGGCGAGGAAGTTGAGAAGCTGGTCGCTGAATTGCCTGAGGGAGGTGTATTGCTGCTTGAAAATGTGAGGTTTTACAAGGAGGAAGAGAAGAATGACCCCGAGTTTGCCAAGAAGCTGGCTTCCCTCGCGGATCTCTATGTTAACGATGCCTTTGGAACTGCTCACAGGGCTCACGCTTCCACAGAGGGTGTGGCTAAGTTCTTGGAACCGTCTGTTGCTGGTTTCCTTATGCAGAAGGTTTGTTGCACGCTCTATGGCCTTTCTTGTTGTGCGTCAAAATGGACATTAACTCTCTAAACGTCAATGGACATTAACTCTCTAACCGTCATGTTTGTTCATTAGTTTTTAGAAGATGACTGATTGCAACTGATGATTCCTTTGGTTCAGGAACTTGACTACCTCGTTGGAGCTGTGGCTAATCCCAAGAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTGTCTTCAAAGATTGGAGTGATTGAATCCTTGTTGGAGAAGGTCAACCTTCTTGTCCTGGGTGGAGGAATGATCTTTACCTTCTACAAGGCGCAAGGTCACTCAGTGGGGTCATCGCTTGTTGAAGAAGACAAGCTTGGCCTTGCTACTTCGCTCATTGAGAAGGCAAAATCCAAGGGGGTGTCTCTTTTGCTCCCAACCGATGTAGTCATAGCAGATAAATTTGCTGCTGATGCTAACAGCAAGGTTCGTAGTTTTGCTTTTTCCAATTGAATATAGCTTTAACATTCATGGAGAGTAGTCGGTAAGAATTTCATCCATAATTTCAGACTGTGGCCGCATCAAGTATCCCAGATGGTTGGATGGGGTTGGATATTGGACCTGATTCTATTAAGAGCTTCAGTGAAGCATTGGACTCTACCCAGACCATTATTTGGAATGGGCCTATGGGAGTGTTTGAGTTTGAGAAGTTTGCGGCTGGAACGGAGGTATGAACATTGGACCAGCTATTTGTTATATATCGATGTTCTTCTGTGGCATGTGTATGTACTCAGTGTTGGGTTGATTTACGCTCATGTGCAGGCAATTGCAAGGAAGTTGGCAGAGCTGAGTGGGAAGGGGGTGACCACCATAATTGGAGGAGGCGACTCTGTTGCGGCTGTTGAGAAGGTTGGGCTTGCGGATAAGATGAGCCACATCTCCACTGGAGGCGGTGCCAGCTTAGAGCTTCTTGAAGGAAAACCGCTTCCCGGGGTCCTTGCTCTCGATGATGCTTGA

mRNA sequence

ATGGCTACCAAAAGGAGCGTTGGTACTCTGAAGGAGGCTGATTTGAAGGGAAAGAGGGTGTTCGTGAGGGTCGATCTCAATGTTCCTTTGGATGACAACTTCAACATCACCGATGACACCAGAATCCGTGCTGCTGTGCCTACCATTAAGTATTTACTCGGCCATGGTGCCAGGGTCATCCTCTCCAGCCACTTGGGGCGCCCAAAGGGCGTAACGCCGAAATACAGTTTGAAACCTCTTGTTCCCAGGCTTTCTGAGCTCCTCGGAGTTCAGGTGAAAATGGCCAATGATTCTATTGGCGAGGAAGTTGAGAAGCTGGTCGCTGAATTGCCTGAGGGAGGTGTATTGCTGCTTGAAAATGTGAGGTTTTACAAGGAGGAAGAGAAGAATGACCCCGAGTTTGCCAAGAAGCTGGCTTCCCTCGCGGATCTCTATGTTAACGATGCCTTTGGAACTGCTCACAGGGCTCACGCTTCCACAGAGGGTGTGGCTAAGTTCTTGGAACCGTCTGTTGCTGGTTTCCTTATGCAGAAGGAACTTGACTACCTCGTTGGAGCTGTGGCTAATCCCAAGAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTGTCTTCAAAGATTGGAGTGATTGAATCCTTGTTGGAGAAGGTCAACCTTCTTGTCCTGGGTGGAGGAATGATCTTTACCTTCTACAAGGCGCAAGGTCACTCAGTGGGGTCATCGCTTGTTGAAGAAGACAAGCTTGGCCTTGCTACTTCGCTCATTGAGAAGGCAAAATCCAAGGGGGTGTCTCTTTTGCTCCCAACCGATGTAGTCATAGCAGATAAATTTGCTGCTGATGCTAACAGCAAGACTGTGGCCGCATCAAGTATCCCAGATGGTTGGATGGGGTTGGATATTGGACCTGATTCTATTAAGAGCTTCAGTGAAGCATTGGACTCTACCCAGACCATTATTTGGAATGGGCCTATGGGAGTGTTTGAGTTTGAGAAGTTTGCGGCTGGAACGGAGGCAATTGCAAGGAAGTTGGCAGAGCTGAGTGGGAAGGGGGTGACCACCATAATTGGAGGAGGCGACTCTGTTGCGGCTGTTGAGAAGGTTGGGCTTGCGGATAAGATGAGCCACATCTCCACTGGAGGCGGTGCCAGCTTAGAGCTTCTTGAAGGAAAACCGCTTCCCGGGGTCCTTGCTCTCGATGATGCTTGA

Coding sequence (CDS)

ATGGCTACCAAAAGGAGCGTTGGTACTCTGAAGGAGGCTGATTTGAAGGGAAAGAGGGTGTTCGTGAGGGTCGATCTCAATGTTCCTTTGGATGACAACTTCAACATCACCGATGACACCAGAATCCGTGCTGCTGTGCCTACCATTAAGTATTTACTCGGCCATGGTGCCAGGGTCATCCTCTCCAGCCACTTGGGGCGCCCAAAGGGCGTAACGCCGAAATACAGTTTGAAACCTCTTGTTCCCAGGCTTTCTGAGCTCCTCGGAGTTCAGGTGAAAATGGCCAATGATTCTATTGGCGAGGAAGTTGAGAAGCTGGTCGCTGAATTGCCTGAGGGAGGTGTATTGCTGCTTGAAAATGTGAGGTTTTACAAGGAGGAAGAGAAGAATGACCCCGAGTTTGCCAAGAAGCTGGCTTCCCTCGCGGATCTCTATGTTAACGATGCCTTTGGAACTGCTCACAGGGCTCACGCTTCCACAGAGGGTGTGGCTAAGTTCTTGGAACCGTCTGTTGCTGGTTTCCTTATGCAGAAGGAACTTGACTACCTCGTTGGAGCTGTGGCTAATCCCAAGAGGCCATTTGCTGCCATTGTTGGTGGTTCAAAGGTGTCTTCAAAGATTGGAGTGATTGAATCCTTGTTGGAGAAGGTCAACCTTCTTGTCCTGGGTGGAGGAATGATCTTTACCTTCTACAAGGCGCAAGGTCACTCAGTGGGGTCATCGCTTGTTGAAGAAGACAAGCTTGGCCTTGCTACTTCGCTCATTGAGAAGGCAAAATCCAAGGGGGTGTCTCTTTTGCTCCCAACCGATGTAGTCATAGCAGATAAATTTGCTGCTGATGCTAACAGCAAGACTGTGGCCGCATCAAGTATCCCAGATGGTTGGATGGGGTTGGATATTGGACCTGATTCTATTAAGAGCTTCAGTGAAGCATTGGACTCTACCCAGACCATTATTTGGAATGGGCCTATGGGAGTGTTTGAGTTTGAGAAGTTTGCGGCTGGAACGGAGGCAATTGCAAGGAAGTTGGCAGAGCTGAGTGGGAAGGGGGTGACCACCATAATTGGAGGAGGCGACTCTGTTGCGGCTGTTGAGAAGGTTGGGCTTGCGGATAAGATGAGCCACATCTCCACTGGAGGCGGTGCCAGCTTAGAGCTTCTTGAAGGAAAACCGCTTCCCGGGGTCCTTGCTCTCGATGATGCTTGA

Protein sequence

MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKELDYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Homology
BLAST of Moc04g00250 vs. NCBI nr
Match: XP_022147931.1 (phosphoglycerate kinase, cytosolic [Momordica charantia])

HSP 1 Score: 772.7 bits (1994), Expect = 1.5e-219
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. NCBI nr
Match: XP_023529234.1 (phosphoglycerate kinase, cytosolic [Cucurbita pepo subsp. pepo] >XP_023529235.1 phosphoglycerate kinase, cytosolic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 746.1 bits (1925), Expect = 1.6e-211
Identity = 384/401 (95.76%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSLIEKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLIEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. NCBI nr
Match: XP_022989221.1 (phosphoglycerate kinase, cytosolic [Cucurbita maxima] >XP_022989222.1 phosphoglycerate kinase, cytosolic [Cucurbita maxima])

HSP 1 Score: 745.3 bits (1923), Expect = 2.6e-211
Identity = 383/401 (95.51%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATS+IEKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSIIEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. NCBI nr
Match: KAG7023014.1 (hypothetical protein SDJN02_16750 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 745.0 bits (1922), Expect = 3.5e-211
Identity = 383/401 (95.51%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. NCBI nr
Match: KAG6589315.1 (hypothetical protein SDJN03_17880, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 743.4 bits (1918), Expect = 1.0e-210
Identity = 382/401 (95.26%), Postives = 394/401 (98.25%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIK FSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKGFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. ExPASy Swiss-Prot
Match: Q42962 (Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 5.0e-205
Identity = 362/401 (90.27%), Postives = 391/401 (97.51%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MA K+SVG+LKEADLKGKRVFVRVDLNVPLD+NFNITDDTRIRAAVPTIKYL+ HG+ VI
Sbjct: 1   MAVKKSVGSLKEADLKGKRVFVRVDLNVPLDENFNITDDTRIRAAVPTIKYLMQHGSHVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           L+SHLGRPKGVTPKYSLKPLVPRLSELLGV+VK+ANDSIG EVEKLVAE+PEGGVLLLEN
Sbjct: 61  LASHLGRPKGVTPKYSLKPLVPRLSELLGVEVKIANDSIGPEVEKLVAEIPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKN+PEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+P+VAGFLMQKEL
Sbjct: 121 VRFYKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPAVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANP++PFAAIVGGSKVSSKIGVIESLLEKV++L+LGGGMIFTFYKAQG++VGS
Sbjct: 181 DYLVGAVANPQKPFAAIVGGSKVSSKIGVIESLLEKVDVLLLGGGMIFTFYKAQGYAVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVVIADKFAADANSK V AS IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLMEKAKAKGVSLLLPTDVVIADKFAADANSKVVPASEIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPD+IKSF  ALD+T+T+IWNGPMGVFEF+KFAAGTEAIA+KLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDAIKSFGSALDTTKTVIWNGPMGVFEFDKFAAGTEAIAKKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLA+KMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLAEKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. ExPASy Swiss-Prot
Match: Q9SAJ4 (Phosphoglycerate kinase 3, cytosolic OS=Arabidopsis thaliana OX=3702 GN=PGK3 PE=1 SV=1)

HSP 1 Score: 706.4 bits (1822), Expect = 1.8e-202
Identity = 361/401 (90.02%), Postives = 387/401 (96.51%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGK VFVRVDLNVPLDDN NITDDTRIRAAVPTIKYL+G+G+RV+
Sbjct: 1   MATKRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVV 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           L SHLGRPKGVTPKYSLKPLVPRLSELLGV+V MANDSIGEEV+KLVA LPEGGVLLLEN
Sbjct: 61  LCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFY EEEKNDPEFAKKLA+LAD+YVNDAFGTAHRAHASTEGVAKFL+PSVAGFLMQKEL
Sbjct: 121 VRFYAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANPK+PFAAIVGGSKVS+KIGVIESLL  V++L+LGGGMIFTFYKAQG SVGS
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LA SL+EKAK+KGVSLLLPTDVVIADKFA DANSK V A++IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIK+FSEALD+T+TIIWNGPMGVFEF+KFAAGTEA+A++LAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of Moc04g00250 vs. ExPASy Swiss-Prot
Match: P12783 (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum OX=4565 PE=2 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 1.4e-194
Identity = 347/401 (86.53%), Postives = 376/401 (93.77%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTL EADLKGK+VFVR DLNVPLDD   ITDDTRIRA++PTIKYLL  GA+VI
Sbjct: 1   MATKRSVGTLGEADLKGKKVFVRADLNVPLDDAQKITDDTRIRASIPTIKYLLEKGAKVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           L+SHLGRPKGVTPK+SLKPLV RLSELLG++V MA D IGEEVEKL A LP+GGVLLLEN
Sbjct: 61  LASHLGRPKGVTPKFSLKPLVARLSELLGLEVVMAPDCIGEEVEKLAAALPDGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLAS+ADLYVNDAFGTAHRAHASTEGV KFL PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASVADLYVNDAFGTAHRAHASTEGVTKFLRPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANPK+PFAAIVGGSKVSSKIGVIESLL KV++L+LGGGMIFTFYKAQG +VG 
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSSKIGVIESLLAKVDILILGGGMIFTFYKAQGLAVGK 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSLIE AKSKGV LLLPTDVV+ADKFAADA SK V A++IPDGWMGLD+
Sbjct: 241 SLVEEDKLELATSLIETAKSKGVKLLLPTDVVVADKFAADAESKIVPATAIPDGWMGLDV 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIK+F+EALD+T+T+IWNGPMGVFEFEKFAAGT+AIA++LAEL+GKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFAEALDTTKTVIWNGPMGVFEFEKFAAGTDAIAKQLAELTGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEK GLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKAGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of Moc04g00250 vs. ExPASy Swiss-Prot
Match: P29409 (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 PE=2 SV=2)

HSP 1 Score: 671.8 bits (1732), Expect = 4.9e-192
Identity = 344/398 (86.43%), Postives = 373/398 (93.72%), Query Frame = 0

Query: 4   KRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSS 63
           K+SVG L  ADLKGK+VFVR DLNVPLDD+ NITDDTRIRAA+PTIK+L+ +GA+VILSS
Sbjct: 31  KKSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAKVILSS 90

Query: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRF 123
           HLGRPKGVTPK+SL PLVPRLSELLG+QV  A+D IG +VEKLVAELPEGGVLLLENVRF
Sbjct: 91  HLGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEKLVAELPEGGVLLLENVRF 150

Query: 124 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKELDYL 183
           YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGV KFL+PSVAGFL+QKELDYL
Sbjct: 151 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 210

Query: 184 VGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLV 243
           VGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SVGSSLV
Sbjct: 211 VGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGMSVGSSLV 270

Query: 244 EEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPD 303
           EEDKL LATSL+ KAK KGVSLLLPTDVVIADKFAADA+SK V AS IPDGWMGLDIGPD
Sbjct: 271 EEDKLDLATSLLAKAKEKGVSLLLPTDVVIADKFAADADSKIVPASGIPDGWMGLDIGPD 330

Query: 304 SIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAA 363
           SIK+FSEALD+TQT+IWNGPMGVFEFEKFAAGTEAIA+KL E+S KG TTIIGGGDSVAA
Sbjct: 331 SIKTFSEALDTTQTVIWNGPMGVFEFEKFAAGTEAIAKKLEEISKKGATTIIGGGDSVAA 390

Query: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VEKVG+A+ MSHISTGGGASLELLEGK LPGVLAL++A
Sbjct: 391 VEKVGVAEAMSHISTGGGASLELLEGKQLPGVLALNEA 428

BLAST of Moc04g00250 vs. ExPASy Swiss-Prot
Match: Q42961 (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 1.9e-191
Identity = 341/398 (85.68%), Postives = 373/398 (93.72%), Query Frame = 0

Query: 4   KRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSS 63
           K+SVG L  A+LKGK+VFVR DLNVPLDDN NITDDTRIRAAVPTIK+L+ +GA+VILSS
Sbjct: 78  KKSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAKVILSS 137

Query: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRF 123
           HLGRPKGVTPKYSL PLVPRLSELLG+QV    D IG EVEKLVA LPEGGVLLLENVRF
Sbjct: 138 HLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEKLVASLPEGGVLLLENVRF 197

Query: 124 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKELDYL 183
           YKEEEKN+PEFAKKLASLADLYVNDAFGTAHRAHASTEGV KFL+PSVAGFL+QKELDYL
Sbjct: 198 YKEEEKNEPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 257

Query: 184 VGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLV 243
           VGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SVGSSLV
Sbjct: 258 VGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 317

Query: 244 EEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPD 303
           EEDKL LATSL+EKAK+KGVSLLLP+DVVIADKFA DANSK V AS+IPDGWMGLDIGPD
Sbjct: 318 EEDKLELATSLLEKAKAKGVSLLLPSDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPD 377

Query: 304 SIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAA 363
           S+K+F++ALD+T+T+IWNGPMGVFEF+KFA GTEAIA+KLA+LSGKGVTTIIGGGDSVAA
Sbjct: 378 SVKTFNDALDTTKTVIWNGPMGVFEFDKFAVGTEAIAKKLADLSGKGVTTIIGGGDSVAA 437

Query: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 438 VEKVGVASVMSHISTGGGASLELLEGKVLPGVIALDEA 475

BLAST of Moc04g00250 vs. ExPASy TrEMBL
Match: A0A6J1D3U8 (Phosphoglycerate kinase OS=Momordica charantia OX=3673 GN=LOC111016741 PE=3 SV=1)

HSP 1 Score: 772.7 bits (1994), Expect = 7.5e-220
Identity = 401/401 (100.00%), Postives = 401/401 (100.00%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. ExPASy TrEMBL
Match: A0A6J1JF79 (Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111486363 PE=3 SV=1)

HSP 1 Score: 745.3 bits (1923), Expect = 1.3e-211
Identity = 383/401 (95.51%), Postives = 395/401 (98.50%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATS+IEKAK+KGVSLLLPTDVVIADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSIIEKAKAKGVSLLLPTDVVIADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. ExPASy TrEMBL
Match: A0A6J1EXD4 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111437038 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 8.3e-211
Identity = 382/401 (95.26%), Postives = 394/401 (98.25%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLK KRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLLGHGARVI
Sbjct: 1   MATKRSVGTLKEADLKRKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLGHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LS+HLGRPKGVTPKYSLKPL+PRLSELLG+QV MAND IGEEVEKLVAEL EGGVLLLEN
Sbjct: 61  LSTHLGRPKGVTPKYSLKPLIPRLSELLGLQVIMANDCIGEEVEKLVAELSEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQGHSVGS
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGHSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSLIEKAK+KGVSLLLPTDVV+ADKFAADANSK VAAS+IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLIEKAKAKGVSLLLPTDVVVADKFAADANSKVVAASAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQT+IWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTVIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 401

BLAST of Moc04g00250 vs. ExPASy TrEMBL
Match: A0A0A0K441 (Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_7G239010 PE=3 SV=1)

HSP 1 Score: 736.5 bits (1900), Expect = 6.0e-209
Identity = 380/401 (94.76%), Postives = 392/401 (97.76%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATK+SVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTI+YLL HGARVI
Sbjct: 1   MATKKSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIQYLLSHGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LSSHLGRPKGVTPKYSLKPLVPRLSELLG+QV MAND IGEEVEK+VAELPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGLQVVMANDCIGEEVEKMVAELPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQG +VG 
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGLAVGK 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSLIEKAK+KGVSLLLPTDVV+ADKFAADA+SK VAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLIEKAKAKGVSLLLPTDVVVADKFAADADSKVVAASSIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSF EALDSTQTIIWNGPMGVFEFEKFAAGTEAIA+KLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFGEALDSTQTIIWNGPMGVFEFEKFAAGTEAIAKKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGK LPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKTLPGVLALDDA 401

BLAST of Moc04g00250 vs. ExPASy TrEMBL
Match: A0A6J1GM56 (Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455236 PE=3 SV=1)

HSP 1 Score: 734.9 bits (1896), Expect = 1.7e-208
Identity = 381/401 (95.01%), Postives = 391/401 (97.51%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLG+GARVI
Sbjct: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGNGARVI 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           LSSHLGRPKGVTPKYSLKPLVPRLSELLGV V MAND IGEEVEKLVA LPEGGVLLLEN
Sbjct: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVSVVMANDCIGEEVEKLVAGLPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAK+L+PSVAGFLMQKEL
Sbjct: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKYLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEKVNLL LGGGMIFTFYKAQG +VG 
Sbjct: 181 DYLVGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLFLGGGMIFTFYKAQGLAVGK 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LATSL+EKAK+KGVSLLLPTDVV+ADKFAADA+SK VAASSIPDGWMGLDI
Sbjct: 241 SLVEEDKLDLATSLLEKAKAKGVSLLLPTDVVVADKFAADADSKVVAASSIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIKSFSEALDSTQTIIWNGPMGVFEF+KFAAGTEAIARKLAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFDKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGK LPGVLALDDA
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKTLPGVLALDDA 401

BLAST of Moc04g00250 vs. TAIR 10
Match: AT1G79550.1 (phosphoglycerate kinase )

HSP 1 Score: 706.4 bits (1822), Expect = 1.3e-203
Identity = 361/401 (90.02%), Postives = 387/401 (96.51%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGK VFVRVDLNVPLDDN NITDDTRIRAAVPTIKYL+G+G+RV+
Sbjct: 1   MATKRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVV 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           L SHLGRPKGVTPKYSLKPLVPRLSELLGV+V MANDSIGEEV+KLVA LPEGGVLLLEN
Sbjct: 61  LCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFY EEEKNDPEFAKKLA+LAD+YVNDAFGTAHRAHASTEGVAKFL+PSVAGFLMQKEL
Sbjct: 121 VRFYAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANPK+PFAAIVGGSKVS+KIGVIESLL  V++L+LGGGMIFTFYKAQG SVGS
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LA SL+EKAK+KGVSLLLPTDVVIADKFA DANSK V A++IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIK+FSEALD+T+TIIWNGPMGVFEF+KFAAGTEA+A++LAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of Moc04g00250 vs. TAIR 10
Match: AT1G79550.2 (phosphoglycerate kinase )

HSP 1 Score: 706.4 bits (1822), Expect = 1.3e-203
Identity = 361/401 (90.02%), Postives = 387/401 (96.51%), Query Frame = 0

Query: 1   MATKRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVI 60
           MATKRSVGTLKEADLKGK VFVRVDLNVPLDDN NITDDTRIRAAVPTIKYL+G+G+RV+
Sbjct: 1   MATKRSVGTLKEADLKGKSVFVRVDLNVPLDDNSNITDDTRIRAAVPTIKYLMGNGSRVV 60

Query: 61  LSSHLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLEN 120
           L SHLGRPKGVTPKYSLKPLVPRLSELLGV+V MANDSIGEEV+KLVA LPEGGVLLLEN
Sbjct: 61  LCSHLGRPKGVTPKYSLKPLVPRLSELLGVEVVMANDSIGEEVQKLVAGLPEGGVLLLEN 120

Query: 121 VRFYKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKEL 180
           VRFY EEEKNDPEFAKKLA+LAD+YVNDAFGTAHRAHASTEGVAKFL+PSVAGFLMQKEL
Sbjct: 121 VRFYAEEEKNDPEFAKKLAALADVYVNDAFGTAHRAHASTEGVAKFLKPSVAGFLMQKEL 180

Query: 181 DYLVGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGS 240
           DYLVGAVANPK+PFAAIVGGSKVS+KIGVIESLL  V++L+LGGGMIFTFYKAQG SVGS
Sbjct: 181 DYLVGAVANPKKPFAAIVGGSKVSTKIGVIESLLNTVDILLLGGGMIFTFYKAQGLSVGS 240

Query: 241 SLVEEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDI 300
           SLVEEDKL LA SL+EKAK+KGVSLLLPTDVVIADKFA DANSK V A++IPDGWMGLDI
Sbjct: 241 SLVEEDKLDLAKSLMEKAKAKGVSLLLPTDVVIADKFAPDANSKIVPATAIPDGWMGLDI 300

Query: 301 GPDSIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDS 360
           GPDSIK+FSEALD+T+TIIWNGPMGVFEF+KFAAGTEA+A++LAELSGKGVTTIIGGGDS
Sbjct: 301 GPDSIKTFSEALDTTKTIIWNGPMGVFEFDKFAAGTEAVAKQLAELSGKGVTTIIGGGDS 360

Query: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALD+A
Sbjct: 361 VAAVEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDEA 401

BLAST of Moc04g00250 vs. TAIR 10
Match: AT3G12780.1 (phosphoglycerate kinase 1 )

HSP 1 Score: 662.5 bits (1708), Expect = 2.1e-190
Identity = 340/398 (85.43%), Postives = 367/398 (92.21%), Query Frame = 0

Query: 4   KRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSS 63
           K+SVG L  ADLKGK+VFVR DLNVPLDDN  ITDDTRIRAA+PTIKYL+ +GA+VILS+
Sbjct: 79  KKSVGDLTSADLKGKKVFVRADLNVPLDDNQTITDDTRIRAAIPTIKYLIENGAKVILST 138

Query: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRF 123
           HLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IG EVE LVA LPEGGVLLLENVRF
Sbjct: 139 HLGRPKGVTPKFSLAPLVPRLSELLGIEVTKADDCIGPEVESLVASLPEGGVLLLENVRF 198

Query: 124 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKELDYL 183
           YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGV KFL+PSVAGFL+QKELDYL
Sbjct: 199 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 258

Query: 184 VGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLV 243
           VGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SVGSSLV
Sbjct: 259 VGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 318

Query: 244 EEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPD 303
           EEDKL LAT L+ KAK+KGVSLLLPTDVV+ADKFA DANSK V AS I DGWMGLDIGPD
Sbjct: 319 EEDKLELATELLAKAKAKGVSLLLPTDVVVADKFAPDANSKIVPASGIEDGWMGLDIGPD 378

Query: 304 SIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAA 363
           SIK+F+EALD+TQT+IWNGPMGVFE EKFAAGTEAIA KLAELS KGVTTIIGGGDSVAA
Sbjct: 379 SIKTFNEALDTTQTVIWNGPMGVFEMEKFAAGTEAIANKLAELSEKGVTTIIGGGDSVAA 438

Query: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 439 VEKVGVAGVMSHISTGGGASLELLEGKVLPGVIALDEA 476

BLAST of Moc04g00250 vs. TAIR 10
Match: AT1G56190.1 (Phosphoglycerate kinase family protein )

HSP 1 Score: 661.0 bits (1704), Expect = 6.1e-190
Identity = 336/398 (84.42%), Postives = 370/398 (92.96%), Query Frame = 0

Query: 4   KRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSS 63
           K+SVG L   DLKGK+VFVR DLNVPLDDN NITDDTRIRAA+PTIK+L+ +GA+VILS+
Sbjct: 76  KKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILST 135

Query: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRF 123
           HLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IG EVE LVA LPEGGVLLLENVRF
Sbjct: 136 HLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRF 195

Query: 124 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKELDYL 183
           YKEEEKN+P+FAKKLASLADLYVNDAFGTAHRAHASTEGV KFL+PSVAGFL+QKELDYL
Sbjct: 196 YKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 255

Query: 184 VGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLV 243
           VGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SVGSSLV
Sbjct: 256 VGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 315

Query: 244 EEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPD 303
           EEDKL LAT+L+ KAK++GVSLLLPTDVVIADKFA DANSK V AS+IPDGWMGLDIGPD
Sbjct: 316 EEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPD 375

Query: 304 SIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAA 363
           S+K+F+EALD+TQT+IWNGPMGVFEFEKFA GTEA+A KLAELS KGVTTIIGGGDSVAA
Sbjct: 376 SVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAA 435

Query: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 436 VEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEA 473

BLAST of Moc04g00250 vs. TAIR 10
Match: AT1G56190.2 (Phosphoglycerate kinase family protein )

HSP 1 Score: 661.0 bits (1704), Expect = 6.1e-190
Identity = 336/398 (84.42%), Postives = 370/398 (92.96%), Query Frame = 0

Query: 4   KRSVGTLKEADLKGKRVFVRVDLNVPLDDNFNITDDTRIRAAVPTIKYLLGHGARVILSS 63
           K+SVG L   DLKGK+VFVR DLNVPLDDN NITDDTRIRAA+PTIK+L+ +GA+VILS+
Sbjct: 3   KKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILST 62

Query: 64  HLGRPKGVTPKYSLKPLVPRLSELLGVQVKMANDSIGEEVEKLVAELPEGGVLLLENVRF 123
           HLGRPKGVTPK+SL PLVPRLSELLG++V  A+D IG EVE LVA LPEGGVLLLENVRF
Sbjct: 63  HLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVETLVASLPEGGVLLLENVRF 122

Query: 124 YKEEEKNDPEFAKKLASLADLYVNDAFGTAHRAHASTEGVAKFLEPSVAGFLMQKELDYL 183
           YKEEEKN+P+FAKKLASLADLYVNDAFGTAHRAHASTEGV KFL+PSVAGFL+QKELDYL
Sbjct: 123 YKEEEKNEPDFAKKLASLADLYVNDAFGTAHRAHASTEGVTKFLKPSVAGFLLQKELDYL 182

Query: 184 VGAVANPKRPFAAIVGGSKVSSKIGVIESLLEKVNLLVLGGGMIFTFYKAQGHSVGSSLV 243
           VGAV+NPKRPFAAIVGGSKVSSKIGVIESLLEK ++L+LGGGMIFTFYKAQG SVGSSLV
Sbjct: 183 VGAVSNPKRPFAAIVGGSKVSSKIGVIESLLEKCDILLLGGGMIFTFYKAQGLSVGSSLV 242

Query: 244 EEDKLGLATSLIEKAKSKGVSLLLPTDVVIADKFAADANSKTVAASSIPDGWMGLDIGPD 303
           EEDKL LAT+L+ KAK++GVSLLLPTDVVIADKFA DANSK V AS+IPDGWMGLDIGPD
Sbjct: 243 EEDKLELATTLLAKAKARGVSLLLPTDVVIADKFAPDANSKIVPASAIPDGWMGLDIGPD 302

Query: 304 SIKSFSEALDSTQTIIWNGPMGVFEFEKFAAGTEAIARKLAELSGKGVTTIIGGGDSVAA 363
           S+K+F+EALD+TQT+IWNGPMGVFEFEKFA GTEA+A KLAELS KGVTTIIGGGDSVAA
Sbjct: 303 SVKTFNEALDTTQTVIWNGPMGVFEFEKFAKGTEAVANKLAELSKKGVTTIIGGGDSVAA 362

Query: 364 VEKVGLADKMSHISTGGGASLELLEGKPLPGVLALDDA 402
           VEKVG+A  MSHISTGGGASLELLEGK LPGV+ALD+A
Sbjct: 363 VEKVGVAGVMSHISTGGGASLELLEGKVLPGVVALDEA 400

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022147931.11.5e-219100.00phosphoglycerate kinase, cytosolic [Momordica charantia][more]
XP_023529234.11.6e-21195.76phosphoglycerate kinase, cytosolic [Cucurbita pepo subsp. pepo] >XP_023529235.1 ... [more]
XP_022989221.12.6e-21195.51phosphoglycerate kinase, cytosolic [Cucurbita maxima] >XP_022989222.1 phosphogly... [more]
KAG7023014.13.5e-21195.51hypothetical protein SDJN02_16750 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589315.11.0e-21095.26hypothetical protein SDJN03_17880, partial [Cucurbita argyrosperma subsp. sorori... [more]
Match NameE-valueIdentityDescription
Q429625.0e-20590.27Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Q9SAJ41.8e-20290.02Phosphoglycerate kinase 3, cytosolic OS=Arabidopsis thaliana OX=3702 GN=PGK3 PE=... [more]
P127831.4e-19486.53Phosphoglycerate kinase, cytosolic OS=Triticum aestivum OX=4565 PE=2 SV=1[more]
P294094.9e-19286.43Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea OX=3562 P... [more]
Q429611.9e-19185.68Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum OX=4097 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1D3U87.5e-220100.00Phosphoglycerate kinase OS=Momordica charantia OX=3673 GN=LOC111016741 PE=3 SV=1[more]
A0A6J1JF791.3e-21195.51Phosphoglycerate kinase OS=Cucurbita maxima OX=3661 GN=LOC111486363 PE=3 SV=1[more]
A0A6J1EXD48.3e-21195.26Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111437038 PE=3 SV=1[more]
A0A0A0K4416.0e-20994.76Phosphoglycerate kinase OS=Cucumis sativus OX=3659 GN=Csa_7G239010 PE=3 SV=1[more]
A0A6J1GM561.7e-20895.01Phosphoglycerate kinase OS=Cucurbita moschata OX=3662 GN=LOC111455236 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G79550.11.3e-20390.02phosphoglycerate kinase [more]
AT1G79550.21.3e-20390.02phosphoglycerate kinase [more]
AT3G12780.12.1e-19085.43phosphoglycerate kinase 1 [more]
AT1G56190.16.1e-19084.42Phosphoglycerate kinase family protein [more]
AT1G56190.26.1e-19084.42Phosphoglycerate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001576Phosphoglycerate kinasePRINTSPR00477PHGLYCKINASEcoord: 113..128
score: 70.19
coord: 317..342
score: 59.85
coord: 141..163
score: 70.95
coord: 172..194
score: 55.09
coord: 195..214
score: 60.33
coord: 376..393
score: 84.62
coord: 36..58
score: 55.95
coord: 353..364
score: 62.91
coord: 14..30
score: 69.62
IPR001576Phosphoglycerate kinasePFAMPF00162PGKcoord: 11..390
e-value: 1.0E-165
score: 551.3
IPR001576Phosphoglycerate kinasePIRSFPIRSF000724Pgkcoord: 2..401
e-value: 1.1E-156
score: 519.5
IPR001576Phosphoglycerate kinasePANTHERPTHR11406PHOSPHOGLYCERATE KINASEcoord: 1..401
IPR001576Phosphoglycerate kinaseHAMAPMF_00145Phosphoglyc_kinasecoord: 9..400
score: 42.67218
IPR001576Phosphoglycerate kinaseCDDcd00318Phosphoglycerate_kinasecoord: 10..400
e-value: 0.0
score: 622.352
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 187..400
e-value: 5.6E-91
score: 305.7
IPR015824Phosphoglycerate kinase, N-terminalGENE3D3.40.50.1260coord: 8..184
e-value: 1.3E-79
score: 268.0
NoneNo IPR availablePIRSRPIRSR000724-2PIRSR000724-2coord: 9..241
e-value: 1.2E-58
score: 196.3
NoneNo IPR availablePANTHERPTHR11406:SF25PHOSPHOGLYCERATE KINASE, CYTOSOLICcoord: 1..401
IPR015911Phosphoglycerate kinase, conserved sitePROSITEPS00111PGLYCERATE_KINASEcoord: 19..29
IPR036043Phosphoglycerate kinase superfamilySUPERFAMILY53748Phosphoglycerate kinasecoord: 8..400

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc04g00250.1Moc04g00250.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006094 gluconeogenesis
biological_process GO:0006096 glycolytic process
cellular_component GO:0005829 cytosol
molecular_function GO:0043531 ADP binding
molecular_function GO:0005524 ATP binding
molecular_function GO:0004618 phosphoglycerate kinase activity