Moc03g31190 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g31190
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	E3 ubiquitin-protein ligase CIP8-like
Location	chr3: 22137115 .. 22137771 (-)
RNA-Seq Expression	Moc03g31190
Synteny	Moc03g31190

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGGCCGCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGGGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAAGAACAAGAAGAACAATAA

mRNA sequence

ATGTCGGCCGCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGGGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAAGAACAAGAAGAACAATAA

Coding sequence (CDS)

ATGTCGGCCGCCGGCCTCCACTGCGAGGTGTGGCAGCCCCAAGAGTGGGCCGAATCAGACTGCTCCTCCTCCTCTTCCGAACCCCCCGAATTCTTCATCGATCTGTACGTCTCCTCCTACGCAAATCAAGGGGGCGAAACAATCAGCAGTCTCCGATACAAGAATTTCCGCCGGCGACGCGACGTTTTCACCCAAGATTCCTTGTCCTGGTCCGCAATTTCCACCATGCTCTCCGAAACCAGCGTGCCCTACCATCTCCAGCCCTTCTTCATCCAACACATTTCCCTTAGGGCTCGCCGAATCGCCGCCCAGCCCACCAACGCCCTCTCCCGCACCATCCCCATCGTCGTCGAGCTCGTCCTGCCACTGCCGGAGGAGGAGGAATCCGGTTCCGGGTCGGATCCCCGGGTTCCGAACGGGCCGGGCCGGGCGAGTAGGGCTTCGATTGAGGAGATGGAGAGGGCGGAAATCGAATTGGGGATTTGTGGCGGTGATTGCGCCATCTGTTTGGATGAGATTGAATGTGGCGCCGTTGGGATGCCCTGTTCGCATATTTACCATCGGAATTGCATTGTGAAATGGCTGCAGCAGAACAATCTTTGCCCCTTGTGTCGGTTTCAGATGCCACTGGAAATGGAAGAACAAGAAGAACAATAA

Protein sequence

MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ

Homology

[Close]

BLAST of Moc03g31190 vs. NCBI nr
Match: XP_022137468.1 (E3 ubiquitin-protein ligase CIP8-like [Momordica charantia])

HSP 1 Score: 446.4 bits (1147), Expect = 1.4e-121
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60
           MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR
Sbjct: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV
Sbjct: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGM 180
           LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGM
Sbjct: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGM 180

Query: 181 PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ 219
           PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ
Sbjct: 181 PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ 218

[Close]

BLAST of Moc03g31190 vs. NCBI nr
Match: KAG6584266.1 (E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 287.7 bits (735), Expect = 8.2e-74
Identity = 151/219 (68.95%), Postives = 172/219 (78.54%), Query Frame = 0


Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 181
           VLP    E+SG GSD  +  G  RASRASI+EMER E+  G+   DC ICLD+I     E
Sbjct: 129 VLPEEAMEDSGYGSDTIMGTGSRRASRASIQEMERVEMN-GVL-SDCVICLDDIVSVGSE 188

Query: 182 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
             A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Moc03g31190 vs. NCBI nr
Match: XP_008437461.1 (PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ubiquitin-protein ligase [Cucumis melo var. makuwa])

HSP 1 Score: 286.6 bits (732), Expect = 1.8e-73
Identity = 153/225 (68.00%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MSAA----GLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGGETISSLRY 60
           MSAA    GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 61  KNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTI 120
           KNFR+R DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 121 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI 180
           P+VVEL+LP    E SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEID-GVL-SDCVICLDEI 180

Query: 181 -----ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
                E  AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 181 GSIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Moc03g31190 vs. NCBI nr
Match: XP_023001523.1 (uncharacterized protein LOC111495638 [Cucurbita maxima])

HSP 1 Score: 286.2 bits (731), Expect = 2.4e-73
Identity = 149/219 (68.04%), Postives = 170/219 (77.63%), Query Frame = 0


Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 181
           VLP    E+S  GSD  +  G  RASRASI+EMER E+   +   DC ICLD+I     E
Sbjct: 129 VLPEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGAL--SDCVICLDDIVSVGSE 188

Query: 182 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
             A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Moc03g31190 vs. NCBI nr
Match: KAG7019862.1 (E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 286.2 bits (731), Expect = 2.4e-73
Identity = 150/219 (68.49%), Postives = 171/219 (78.08%), Query Frame = 0


Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 181
           VLP    E+SG GSD  +  G  RASR SI+EMER E+  G+   DC ICLD+I     E
Sbjct: 129 VLPEEAMEDSGYGSDTIMGTGSRRASRTSIQEMERVEMN-GVL-SDCVICLDDIVSVGSE 188

Query: 182 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
             A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Moc03g31190 vs. ExPASy Swiss-Prot
Match: O22197 (Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RHC1A PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 7.9e-11
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 127 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 186
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 187 IYHRNCIVKWLQQNNLCPLCRFQMP 210
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of Moc03g31190 vs. ExPASy Swiss-Prot
Match: Q9SNB6 (E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 1.8e-10
Identity = 42/106 (39.62%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIE--LGICGGDCAICLDEIECG 178
           L+  L + E SG+G   R  N P  AS+++IE + R EI         +CA+C +  E G
Sbjct: 169 LLEQLSQIEASGNGIG-RSGNPP--ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAG 228

Query: 179 AVG--MPCSHIYHRNCIVKWLQQNNLCPLCRFQM---PLEMEEQEE 218
             G  MPC HI+H +CIV WL   N CP+CRF++   P++   +EE
Sbjct: 229 IEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSDPIQRSNEEE 271

[Close]

BLAST of Moc03g31190 vs. ExPASy Swiss-Prot
Match: Q6AVN2 (E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SIRP1 PE=1 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 2.3e-10
Identity = 32/81 (39.51%), Postives = 51/81 (62.96%), Query Frame = 0


Query: 133 SDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSHIYHRNC 192
           SDP   NG   A + ++E +   +IE  +    C++CLD++E G  A  MPC H +H +C
Sbjct: 170 SDPS-RNGTPPAKKEAVEALPTVKIEEVV---SCSVCLDDLEVGSQAKQMPCEHKFHSSC 229

Query: 193 IVKWLQQNNLCPLCRFQMPLE 212
           I+ WL+ ++ CP+CRF++P E
Sbjct: 230 ILPWLELHSSCPVCRFELPSE 246

[Close]

BLAST of Moc03g31190 vs. ExPASy Swiss-Prot
Match: Q940T5 (E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.9e-10
Identity = 40/97 (41.24%), Postives = 56/97 (57.73%), Query Frame = 0


Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGD--CAICLD--EIE 178
           L+  L + E S +G   R  N P  AS+++IE + R EI     G +  CA+C +  E E
Sbjct: 153 LLEQLSQIEASATGIG-RSGNPP--ASKSAIESLPRVEISDCHIGSEANCAVCTEIFETE 212

Query: 179 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLE 212
             A  MPC H++H +CIV WL   N CP+CRF++P E
Sbjct: 213 TEAREMPCKHLFHDDCIVPWLSIRNSCPVCRFELPSE 246

[Close]

BLAST of Moc03g31190 vs. ExPASy Swiss-Prot
Match: Q8LPN7 (E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19950 PE=1 SV=1)

HSP 1 Score: 66.6 bits (161), Expect = 3.9e-10
Identity = 32/110 (29.09%), Postives = 64/110 (58.18%), Query Frame = 0


Query: 114 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGD---CAICL 173
           P + +L+  L E + +  G+ P        AS+++I+ +   ++   +   +   CA+C+
Sbjct: 169 PGLEQLIQQLAENDPNRYGTPP--------ASKSAIDALPTVKVTKDMLKSEMNQCAVCM 228

Query: 174 DEIECGA--VGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ 219
           DE E G+    MPC H++H++C++ WL+ +N CP+CRF++P +  + E +
Sbjct: 229 DEFEDGSDVKQMPCKHVFHQDCLLPWLELHNSCPVCRFELPTDDPDYENR 270

[Close]

BLAST of Moc03g31190 vs. ExPASy TrEMBL
Match: A0A6J1CAF5 (E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC111008905 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 6.7e-122
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0


Query: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60
           MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR
Sbjct: 1   MSAAGLHCEVWQPQEWAESDCSSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRRR 60

Query: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120
           DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV
Sbjct: 61  DVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVELV 120

Query: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGM 180
           LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGM
Sbjct: 121 LPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECGAVGM 180

Query: 181 PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ 219
           PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ
Sbjct: 181 PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ 218

[Close]

BLAST of Moc03g31190 vs. ExPASy TrEMBL
Match: A0A5D3BJ05 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003020 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.9e-74
Identity = 153/225 (68.00%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MSAA----GLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGGETISSLRY 60
           MSAA    GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 61  KNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTI 120
           KNFR+R DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 121 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI 180
           P+VVEL+LP    E SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEID-GVL-SDCVICLDEI 180

Query: 181 -----ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
                E  AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 181 GSIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Moc03g31190 vs. ExPASy TrEMBL
Match: A0A1S3AU71 (uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=4 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 8.9e-74
Identity = 153/225 (68.00%), Postives = 173/225 (76.89%), Query Frame = 0


Query: 1   MSAA----GLHCEVWQPQEWAESDCSSSSS---EPPEFFIDLYVSSYANQGGETISSLRY 60
           MSAA    GLHCEVWQPQ+++E  CSSSSS   +PPEFFID YVSSYA  G E IS+LRY
Sbjct: 1   MSAAAAISGLHCEVWQPQDFSEPSCSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRY 60

Query: 61  KNFRRRRDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTI 120
           KNFR+R DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS+RAR IA +P NALSRTI
Sbjct: 61  KNFRQRCDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISIRARGIATEPINALSRTI 120

Query: 121 PIVVELVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI 180
           P+VVEL+LP    E SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI
Sbjct: 121 PMVVELMLPEDAMEGSGYGSEPHMGIGSGRASRASIQEMERIEID-GVL-SDCVICLDEI 180

Query: 181 -----ECGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
                E  AV MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 181 GSIGCEIDAVRMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Moc03g31190 vs. ExPASy TrEMBL
Match: A0A6J1KQS3 (uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.2e-73
Identity = 149/219 (68.04%), Postives = 170/219 (77.63%), Query Frame = 0


Query: 2   SAAGLHCEVWQPQEWAESDC--SSSSSEPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 61
           + +GLHCEVWQPQ+++E+ C  SSSSSE PEFFID YVSSYA QG + IS+LRYKNFR+R
Sbjct: 9   AVSGLHCEVWQPQDFSETHCSFSSSSSESPEFFIDFYVSSYATQGDQIISTLRYKNFRQR 68

Query: 62  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 121
            DV TQDS SWSAISTMLSET+VPYHLQPFFIQ IS+ AR IA +PTNALSRTIP+VVEL
Sbjct: 69  CDVLTQDSFSWSAISTMLSETNVPYHLQPFFIQQISISARGIATEPTNALSRTIPMVVEL 128

Query: 122 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 181
           VLP    E+S  GSD  +  G  RASRASI+EMER E+   +   DC ICLD+I     E
Sbjct: 129 VLPEEAMEDSSYGSDTIMGTGSRRASRASIQEMERVEMNGAL--SDCVICLDDIVSVGSE 188

Query: 182 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
             A+ MPC HIYHRNCI KWLQ +N CPLCRFQMPLE E
Sbjct: 189 IDALRMPCLHIYHRNCIHKWLQLSNRCPLCRFQMPLEEE 225

[Close]

BLAST of Moc03g31190 vs. ExPASy TrEMBL
Match: A0A0A0LR28 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 PE=4 SV=1)

HSP 1 Score: 285.4 bits (729), Expect = 2.0e-73
Identity = 148/219 (67.58%), Postives = 169/219 (77.17%), Query Frame = 0


Query: 4   AGLHCEVWQPQEWAESDCSSSSS----EPPEFFIDLYVSSYANQGGETISSLRYKNFRRR 63
           +GLHCEVWQPQ+++E  CSSSSS    +PPEFFID YVSSYA  G E IS+LRYKNFR+R
Sbjct: 7   SGLHCEVWQPQDFSEPSCSSSSSSSQFQPPEFFIDFYVSSYATHGDEVISTLRYKNFRQR 66

Query: 64  RDVFTQDSLSWSAISTMLSETSVPYHLQPFFIQHISLRARRIAAQPTNALSRTIPIVVEL 123
            DV TQDS SWSAIS+MLSET+VPYHLQPFFI  IS RAR IA +P NALSRTIP+VVEL
Sbjct: 67  CDVLTQDSFSWSAISSMLSETNVPYHLQPFFIHQISTRARGIATEPINALSRTIPMVVEL 126

Query: 124 VLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEI-----E 183
           +LP    E+SG GS+P +  G GRASRASI+EMER EI+ G+   DC ICLDEI     E
Sbjct: 127 ILPEDAMEDSGYGSEPHMGIGSGRASRASIQEMERIEID-GVL-SDCVICLDEIGSIGCE 186

Query: 184 CGAVGMPCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEME 214
              V MPC H+YH NCI KWL+ +N CPLCRFQMPLE E
Sbjct: 187 IDVVQMPCLHVYHLNCIHKWLELSNRCPLCRFQMPLEEE 223

[Close]

BLAST of Moc03g31190 vs. TAIR 10
Match: AT1G14200.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 4.3e-12
Identity = 47/158 (29.75%), Postives = 76/158 (48.10%), Query Frame = 0


Query: 90  FIQHISLRARRIAAQPTNALSRTIPIVVELVLPLPEEEESGSG-----SDPRVP------ 149
           F++  S+  R I  +  + LSR +P++V L     ++++  +       DP  P      
Sbjct: 7   FVEFSSMFERIIQGR-GDGLSRFLPVIVALAAREDDDDQGSTDQTTRRGDPLSPRFVMIG 66

Query: 150 --------------NGPGRASRASIEEMERAEI--ELGICGGDCAICLDEIECG--AVGM 209
                          G   A ++ +E M R  I  +    GG CAICLDE   G  A  M
Sbjct: 67  SRSGLDDFFSDGGKQGRSPALKSEVENMPRVVIGEDKEKYGGSCAICLDEWSKGDVAAEM 126

Query: 210 PCSHIYHRNCIVKWLQQNNLCPLCRFQMPLEMEEQEEQ 219
           PC H +H  C+ +WL ++  CP+CR++MP+E  E+E++
Sbjct: 127 PCKHKFHSKCVEEWLGRHATCPMCRYEMPVEEVEEEKK 163

[Close]

BLAST of Moc03g31190 vs. TAIR 10
Match: AT2G40830.2 (RING-H2 finger C1A )

HSP 1 Score: 68.9 bits (167), Expect = 5.6e-12
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 127 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 186
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 187 IYHRNCIVKWLQQNNLCPLCRFQMP 210
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of Moc03g31190 vs. TAIR 10
Match: AT2G40830.1 (RING-H2 finger C1A )

HSP 1 Score: 68.9 bits (167), Expect = 5.6e-12
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 127 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 186
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 187 IYHRNCIVKWLQQNNLCPLCRFQMP 210
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of Moc03g31190 vs. TAIR 10
Match: AT2G40830.3 (RING-H2 finger C1A )

HSP 1 Score: 68.9 bits (167), Expect = 5.6e-12
Identity = 33/85 (38.82%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 127 EESGSGSDPRVPNGPGRASRASIEEMERAEIELGICGGDCAICLDEIECG--AVGMPCSH 186
           E+  +G+  R P    R++  ++  ++ A+  L     +C +C DE E G  A  MPC+H
Sbjct: 151 EQLSAGTTRRGPPPAPRSAIDALPTIKIAQRHLRSSDSNCPVCKDEFELGSEAKQMPCNH 210

Query: 187 IYHRNCIVKWLQQNNLCPLCRFQMP 210
           IYH +CIV WL Q+N CP+CR ++P
Sbjct: 211 IYHSDCIVPWLVQHNSCPVCRQELP 235

[Close]

BLAST of Moc03g31190 vs. TAIR 10
Match: AT3G46620.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 67.8 bits (164), Expect = 1.2e-11
Identity = 42/106 (39.62%), Postives = 60/106 (56.60%), Query Frame = 0


Query: 119 LVLPLPEEEESGSGSDPRVPNGPGRASRASIEEMERAEIE--LGICGGDCAICLDEIECG 178
           L+  L + E SG+G   R  N P  AS+++IE + R EI         +CA+C +  E G
Sbjct: 169 LLEQLSQIEASGNGIG-RSGNPP--ASKSAIESLPRVEISDCHTKAEANCAVCTEVFEAG 228

Query: 179 AVG--MPCSHIYHRNCIVKWLQQNNLCPLCRFQM---PLEMEEQEE 218
             G  MPC HI+H +CIV WL   N CP+CRF++   P++   +EE
Sbjct: 229 IEGREMPCKHIFHGDCIVPWLSIRNSCPVCRFELPSDPIQRSNEEE 271

The following BLAST results are available for this feature:

BLAST of Moc03g31190 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137468.1	1.4e-121	100.00	E3 ubiquitin-protein ligase CIP8-like [Momordica charantia]	[more]
KAG6584266.1	8.2e-74	68.95	E3 ubiquitin-protein ligase MPSR1, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_008437461.1	1.8e-73	68.00	PREDICTED: uncharacterized protein LOC103482872 [Cucumis melo] >TYJ99097.1 E3 ub...	[more]
XP_023001523.1	2.4e-73	68.04	uncharacterized protein LOC111495638 [Cucurbita maxima]	[more]
KAG7019862.1	2.4e-73	68.49	E3 ubiquitin-protein ligase SIRP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Moc03g31190 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22197	7.9e-11	38.82	Probable E3 ubiquitin-protein ligase RHC1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q9SNB6	1.8e-10	39.62	E3 ubiquitin-protein ligase RDUF1 OS=Arabidopsis thaliana OX=3702 GN=RDUF1 PE=1 ...	[more]
Q6AVN2	2.3e-10	39.51	E3 ubiquitin-protein ligase SIRP1 OS=Oryza sativa subsp. japonica OX=39947 GN=SI...	[more]
Q940T5	3.9e-10	41.24	E3 ubiquitin-protein ligase RDUF2 OS=Arabidopsis thaliana OX=3702 GN=DURF2 PE=1 ...	[more]
Q8LPN7	3.9e-10	29.09	E3 ubiquitin-protein ligase RING1-like OS=Arabidopsis thaliana OX=3702 GN=At3g19...	[more]

BLAST of Moc03g31190 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CAF5	6.7e-122	100.00	E3 ubiquitin-protein ligase CIP8-like OS=Momordica charantia OX=3673 GN=LOC11100...	[more]
A0A5D3BJ05	8.9e-74	68.00	E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf...	[more]
A0A1S3AU71	8.9e-74	68.00	uncharacterized protein LOC103482872 OS=Cucumis melo OX=3656 GN=LOC103482872 PE=...	[more]
A0A6J1KQS3	1.2e-73	68.04	uncharacterized protein LOC111495638 OS=Cucurbita maxima OX=3661 GN=LOC111495638...	[more]
A0A0A0LR28	2.0e-73	67.58	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G045460 P...	[more]

BLAST of Moc03g31190 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G14200.1	4.3e-12	29.75	RING/U-box superfamily protein	[more]
AT2G40830.2	5.6e-12	38.82	RING-H2 finger C1A	[more]
AT2G40830.1	5.6e-12	38.82	RING-H2 finger C1A	[more]
AT2G40830.3	5.6e-12	38.82	RING-H2 finger C1A	[more]
AT3G46620.1	1.2e-11	39.62	zinc finger (C3HC4-type RING finger) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 166..204 e-value: 1.4E-6 score: 37.9
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 165..205 e-value: 2.6E-11 score: 43.7
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 166..205 score: 12.54829
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 136..211 e-value: 2.0E-16 score: 61.4
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 126..146
None	No IPR available	PANTHER	PTHR15710	E3 UBIQUITIN-PROTEIN LIGASE PRAJA	coord: 6..209
None	No IPR available	PANTHER	PTHR15710:SF138	FINGER PROTEIN, PUTATIVE-RELATED	coord: 6..209
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 165..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g31190.1	Moc03g31190.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0061630	ubiquitin protein ligase activity