Moc03g31050 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g31050 vs. NCBI nr
Match: XP_022137619.1 (protein NRT1/ PTR FAMILY 2.8 [Momordica charantia])

HSP 1 Score: 800.8 bits (2067), Expect = 5.4e-228
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV
Sbjct: 192 MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 251

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK
Sbjct: 252 ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 311

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP
Sbjct: 312 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 371

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
           AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE
Sbjct: 372 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 431

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF
Sbjct: 432 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 491

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV
Sbjct: 492 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 551

Query: 361 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKETKEAAAEC 407
           FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKETKEAAAEC
Sbjct: 552 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKETKEAAAEC 597

BLAST of Moc03g31050 vs. NCBI nr
Match: XP_023001041.1 (protein NRT1/ PTR FAMILY 2.8 [Cucurbita maxima])

HSP 1 Score: 619.8 bits (1597), Expect = 1.7e-173
Identity = 296/380 (77.89%), Postives = 344/380 (90.53%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTN+SW +GF IPTICFFFSI  FL GRHTYI+AEPRGS+FSDM RV
Sbjct: 196 VALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYIMAEPRGSMFSDMARV 255

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I AACRKRR+SVSS+SFY+PPMADS   EK+ HT+R+KWLDKAAIIVNPDEELDEQGKPK
Sbjct: 256 IIAACRKRRYSVSSYSFYEPPMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPK 315

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEG KCLV+IIP+W+SG+GCF+VF+QPNT GILQA+Q+NRSIG+HFKFPP
Sbjct: 316 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 375

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  +++LSIWIIIYERVFIKMAKKITGKERRLT+KQRITIGI LSI+CMVVSG+VE
Sbjct: 376 GWMNLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIILSIVCMVVSGIVE 435

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           +YRRE+ALKNGSF S  SFAF+LPQHAL+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 436 RYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 495

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSV+S++SSLIVNL+H+V+ +  + PWVGGHDLN+ RLDYYY+ IAI+ TLNLLYFV
Sbjct: 496 FLTLSVASYLSSLIVNLIHSVSGEFAESPWVGGHDLNENRLDYYYFTIAIVGTLNLLYFV 555

Query: 361 FFASRFVTSYDENTKLKREV 381
            FASRFVTSYD   KL  ++
Sbjct: 556 LFASRFVTSYDNKVKLMEDL 575

BLAST of Moc03g31050 vs. NCBI nr
Match: XP_023520205.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608.2 bits (1567), Expect = 5.1e-170
Identity = 292/380 (76.84%), Postives = 338/380 (88.95%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTN+SW +GF IPTICFFFSI  FL GRHTYI+AEPRGS+FSDM RV
Sbjct: 196 VALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYIMAEPRGSMFSDMARV 255

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I AACRK R+SVSS+SFYDPPMADS   EK+ HT+R+KWLDKAA IVNPDEELDEQGKPK
Sbjct: 256 IIAACRKGRYSVSSYSFYDPPMADSSHEEKLVHTERFKWLDKAAFIVNPDEELDEQGKPK 315

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEG KCLV+IIP+W+SG+GCF+VF+QPNT GILQA+Q+NRSIG+HFKFPP
Sbjct: 316 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 375

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  +++LSIWIIIYERVFIKMAKKITGKERRLT+KQRITIGI LSI+CMVVSG+VE
Sbjct: 376 GWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIILSIVCMVVSGIVE 435

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           +YRRE+ALKNGSF S  SFAF+LPQHAL+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 436 RYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 495

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSV+S++SSLIVNL+  V+ +  +  WVGGHDLN+ RLDYYY+ IAI+  LNLLYFV
Sbjct: 496 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFV 555

Query: 361 FFASRFVTSYDENTKLKREV 381
            FASRFVTSYD   KL  ++
Sbjct: 556 LFASRFVTSYDNKVKLMEDL 575

BLAST of Moc03g31050 vs. NCBI nr
Match: XP_038894640.1 (protein NRT1/ PTR FAMILY 2.8 [Benincasa hispida])

HSP 1 Score: 606.3 bits (1562), Expect = 1.9e-169
Identity = 295/398 (74.12%), Postives = 346/398 (86.93%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALL+ALTGVVYVQTNVSW +GF IPTICFFFSI  FL GRHTYI+AEPRGS+F+DM RV
Sbjct: 191 IALLVALTGVVYVQTNVSWTLGFAIPTICFFFSISIFLMGRHTYIIAEPRGSMFTDMSRV 250

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I AACR+R+HSVSS+SFYDPPM DS  GEK+ HT+R+KWLD+AAIIVNP+EELDEQGKPK
Sbjct: 251 IIAACRRRKHSVSSYSFYDPPMEDSSCGEKLIHTERFKWLDRAAIIVNPEEELDEQGKPK 310

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEGLKCLV+IIPVW+SG+GCFIVF+QPNT GILQAMQ+NRSIG HFKFPP
Sbjct: 311 NPWRLCSLQQVEGLKCLVSIIPVWISGIGCFIVFNQPNTFGILQAMQSNRSIGPHFKFPP 370

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  +++LSIWIIIYERVFIK+ KK TGKERRLT++QRITIGI LSI+ M+VSG+VE
Sbjct: 371 GWMNLAGMISLSIWIIIYERVFIKLGKKFTGKERRLTMEQRITIGIVLSILSMIVSGIVE 430

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           K+RR++ALK GSF S TSFAF++PQHAL+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 431 KHRRDAALKTGSFVSPTSFAFLIPQHALTGLMEAFALVALMEFFTMHMPEHMRTVAGAIF 490

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSV+S++SS +V+++H V++KT K PWVGGHDLNQ RLDYYY  IA+I TLNLLYFV
Sbjct: 491 FLTLSVASYLSSFLVDVIHIVSAKTAKSPWVGGHDLNQNRLDYYYVTIAVIGTLNLLYFV 550

Query: 361 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKE 399
           FFASRFV  YD   KL         TEN  + DLP K+
Sbjct: 551 FFASRFVRGYDSKVKL---------TENVDRSDLPVKD 579

BLAST of Moc03g31050 vs. NCBI nr
Match: KAG7019852.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 605.1 bits (1559), Expect = 4.3e-169
Identity = 290/380 (76.32%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTN+SW +GF IPTICFFFSI  FL GRHTYI+AEPRGS+FSDM RV
Sbjct: 272 VALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYIMAEPRGSMFSDMARV 331

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I +ACRKRR+SVSS+SFYDPPMA S   EK+ HT+R+KWLDKAAIIVNPDEELDEQGKPK
Sbjct: 332 IISACRKRRYSVSSYSFYDPPMAYSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPK 391

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEG KCLV+IIP+W+SG+GCF+VF+QPNT GILQA+Q+NRSIG+HFKFPP
Sbjct: 392 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 451

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  +++LSIWIIIYERVFIKMAKKITGKERRLT+KQRITIGI +SI+CMVVSG+VE
Sbjct: 452 GWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVE 511

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           +YRRE+AL+NGSF S  SFAF+LPQHAL+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 512 RYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 571

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSV+S++SSLIVNL+  V+ +  +  WVGGHDLN+ RLDYYY+ IAI+  LNLLYFV
Sbjct: 572 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFV 631

Query: 361 FFASRFVTSYDENTKLKREV 381
            FASRFVTSYD   KL  ++
Sbjct: 632 LFASRFVTSYDNKVKLMEDL 651

BLAST of Moc03g31050 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 379.0 bits (972), Expect = 6.6e-104
Identity = 198/377 (52.52%), Postives = 270/377 (71.62%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +AL+IALTGVVY+QTN+SW IGFVIPT C   SI  F+ G+HTYI A+  GS+F+D+V+V
Sbjct: 188 VALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKV 247

Query: 61  ITAACRKRRHSVSSH-SFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKP 120
           +TAAC+KR+    S  +FY  P  D      +    R ++ DKA+I+ NP+ EL+E G  
Sbjct: 248 VTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPN-ELNEDGNA 307

Query: 121 KNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSH-FKF 180
           K  WRLCS+QQV+ LKC+ AI+PVWV+G+ CFI+ DQ N  GILQAMQ +++ G H F+ 
Sbjct: 308 KYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQV 367

Query: 181 PPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGV 240
           P  W+ + S++ L+IWI +YE V I + K+ITG+++RLT+K RI   I + I+CM+V+G 
Sbjct: 368 PAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGF 427

Query: 241 VEKYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGA 300
            EK RR SALKNGSF S  S   +LPQ AL+GL EAF+ +A+MEF  + MPEHMR VAGA
Sbjct: 428 QEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGA 487

Query: 301 IFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLY 360
           IFFL+ S++S++ +L++N++ AVT K GK  W+G  DLN+ RL+ Y+++IA I   NLLY
Sbjct: 488 IFFLSSSIASYICTLLINVIDAVTRKEGK-SWLGDKDLNKNRLENYFFIIAGIQVANLLY 547

Query: 361 FVFFASRFVTSYDENTK 376
           F  FASR+ T   EN K
Sbjct: 548 FRLFASRYAT---ENKK 557

BLAST of Moc03g31050 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 1.4e-66
Identity = 139/370 (37.57%), Postives = 226/370 (61.08%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           + L+I  T VVY+Q  VSW IGF IPT     +++ F  G   Y+  +P GSIFS + +V
Sbjct: 223 VVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQV 282

Query: 61  ITAACRKRRHSVSSH-----SFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDE 120
           I AA +KR+  + +      ++YDP +  S+   K+  +++++ LDKAA+++  D  L  
Sbjct: 283 IVAARKKRKLKLPAEDDGTVTYYDPAIKSSV-LSKLHRSNQFRCLDKAAVVIEGD--LTP 342

Query: 121 QGKPKNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSH 180
           +G P + WRLCS+Q+VE +KCL+ I+P+W +G+          T  + QA++ +R++G  
Sbjct: 343 EGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPK 402

Query: 181 FKFPPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVV 240
           F+ P   + + SLL + I++  Y+RVF+   ++ITG +  +T+ QRI  GI  +I  M+V
Sbjct: 403 FEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIV 462

Query: 241 SGVVEKYRRESALKNGSFNSTT--SFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMR 300
           +G+VE+ RR  ++  G     T  S  ++ PQ  L GL EAF  I  +EFFN   PEHMR
Sbjct: 463 AGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMR 522

Query: 301 TVAGAIFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIAT 360
           ++A ++F L+ + SS++SS +V +VH  +    +  W+   +LN  +LDY+YYLIA++  
Sbjct: 523 SIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGV 582

Query: 361 LNLLYFVFFA 364
           +NL+YF + A
Sbjct: 583 VNLVYFWYCA 588

BLAST of Moc03g31050 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 246.5 bits (628), Expect = 5.1e-64
Identity = 141/371 (38.01%), Postives = 225/371 (60.65%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           M L+ + T VVY+QT VSW IGF IPT     +++ F  G   Y+  +P GS+FS + RV
Sbjct: 186 MVLIFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARV 245

Query: 61  ITAACRKRRHSVS-----SHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDE 120
           I AA +KR   +S     +  +Y+PP+   +   K+  TD++K+LDKAA+I+  D +L  
Sbjct: 246 IVAARKKRDLKISLVDDGTEEYYEPPVKPGV-LSKLPLTDQFKFLDKAAVIL--DGDLTS 305

Query: 121 QGKPKNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSH 180
           +G P N WRLCS+Q+VE +KCL+ ++PVW +G+   +      T  + QA + +R +G H
Sbjct: 306 EGVPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPH 365

Query: 181 FKFPPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVV 240
           F+ P A + + S + + IW+ IYE + +    ++  ++ R+T+ QR+ IGI  +I+ M  
Sbjct: 366 FEIPAASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFT 425

Query: 241 SGVVEKYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTV 300
           +G VE  RR  A +       + F   LP   L GL E+F  I ++EFFN   PEHMR++
Sbjct: 426 AGFVEGVRRTRATE---MTQMSVFWLALPL-ILMGLCESFNFIGLIEFFNSQFPEHMRSI 485

Query: 301 AGAIFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLN 360
           A ++F L+ + ++++SSL+V  VH V+       W+   DL++ +LDY+YYLIA++  +N
Sbjct: 486 ANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVN 545

Query: 361 LLYFVFFASRF 367
           L+YF + A R+
Sbjct: 546 LVYFWYCAHRY 545

BLAST of Moc03g31050 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.5e-63
Identity = 140/401 (34.91%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 2   ALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRVI 61
           A +++LT VVYVQ+NVSW IG  IP +  F + L F  G   Y+  +  GS  + + +VI
Sbjct: 216 AQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVI 275

Query: 62  TAACRKR------RHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDE 121
             A +KR      +  ++ +++Y P  A+S    K+ +TD++++LDKAAI+  P+++L  
Sbjct: 276 AVAIKKRGLKPAKQPWLNLYNYYPPKYANS----KLKYTDQFRFLDKAAIL-TPEDKLQP 335

Query: 122 QGKPKNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGS- 181
            GKP +PW+LC++QQVE +KC+V ++P+W +    ++   Q  T  + QA+Q++R +GS 
Sbjct: 336 DGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSG 395

Query: 182 HFKFPPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMV 241
            F  P A   +  +  ++++I++Y+RV +   ++ITG +  +T+ QRI  GIF +   +V
Sbjct: 396 GFVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLV 455

Query: 242 VSGVVEKYRRESALKNGSFN--------STTSFAFMLPQHALSGLMEAFATIAVMEFFNM 301
           V+G VE+ RR  AL   +          S+ S  +++PQ +L+G+ EAFA I  MEF+  
Sbjct: 456 VAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYK 515

Query: 302 NMPEHMRTVAGAIFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYY 361
             PE+MR+ AG+IF++   VSS++ S ++  VH  T  +    W+   DLN+ RLD +Y+
Sbjct: 516 QFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWL-AEDLNKGRLDLFYF 575

Query: 362 LIAIIATLNLLYFVFFASRF--------VTSYDENTKLKRE 380
           +IA I  +N  YF+  +  +        VT+Y+ N  + ++
Sbjct: 576 MIAGILAVNFAYFLVMSRWYRYKGSDDEVTTYETNENIIKQ 610

BLAST of Moc03g31050 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 241.5 bits (615), Expect = 1.6e-62
Identity = 128/365 (35.07%), Postives = 232/365 (63.56%), Query Frame = 0

Query: 4   LIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRVITA 63
           L+++T V+YVQ N+SW IGF IPT+  FF++L    G   Y+  +P GS+FS + +V+ A
Sbjct: 194 LVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVFKVLVA 253

Query: 64  ACRKRRHSVSSHSFYDPPMADS-LDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPKNP 123
           A +KR+   +S   Y  P+ ++ L   K+  TD++++L+KA I++N DE  +E+      
Sbjct: 254 AYKKRKARFTSGIDYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNNDEAGNEE------ 313

Query: 124 WRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSI-GSHFKFPPA 183
           WR C+++Q+E +K +++IIP++ S +  F+  +Q  T  + QA++ +    G+ +  PPA
Sbjct: 314 WRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTSYLIPPA 373

Query: 184 WVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVEK 243
            + + SLL + IW+  YE V ++  + IT +   +++ Q++ IG   SI  M++SG+VE+
Sbjct: 374 SITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLISGIVER 433

Query: 244 YRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIFF 303
            RR+ +L NG      S  ++ PQ  L G  + F  + + EFFN  +P +MR++  ++ +
Sbjct: 434 KRRDLSL-NG---VKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSIGNSLLY 493

Query: 304 LTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFVF 363
           L LS++S++SS +V++VH+VT++ G+  W+   D+++++LD +YY IA ++TLN ++F +
Sbjct: 494 LGLSLASYLSSAMVSIVHSVTARGGQ-SWL-TDDIDKSKLDCFYYFIAALSTLNFIFFFW 546

Query: 364 FASRF 367
            A R+
Sbjct: 554 CARRY 546

BLAST of Moc03g31050 vs. ExPASy TrEMBL
Match: A0A6J1C7R5 (protein NRT1/ PTR FAMILY 2.8 OS=Momordica charantia OX=3673 GN=LOC111009018 PE=3 SV=1)

HSP 1 Score: 800.8 bits (2067), Expect = 2.6e-228
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV
Sbjct: 192 MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 251

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK
Sbjct: 252 ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 311

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP
Sbjct: 312 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 371

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
           AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE
Sbjct: 372 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 431

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF
Sbjct: 432 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 491

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV
Sbjct: 492 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 551

Query: 361 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKETKEAAAEC 407
           FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKETKEAAAEC
Sbjct: 552 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKETKEAAAEC 597

BLAST of Moc03g31050 vs. ExPASy TrEMBL
Match: A0A6J1KHH5 (protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita maxima OX=3661 GN=LOC111495296 PE=3 SV=1)

HSP 1 Score: 619.8 bits (1597), Expect = 8.2e-174
Identity = 296/380 (77.89%), Postives = 344/380 (90.53%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTN+SW +GF IPTICFFFSI  FL GRHTYI+AEPRGS+FSDM RV
Sbjct: 196 VALLIALTGVVYVQTNISWTLGFAIPTICFFFSITIFLLGRHTYIMAEPRGSMFSDMARV 255

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I AACRKRR+SVSS+SFY+PPMADS   EK+ HT+R+KWLDKAAIIVNPDEELDEQGKPK
Sbjct: 256 IIAACRKRRYSVSSYSFYEPPMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPK 315

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEG KCLV+IIP+W+SG+GCF+VF+QPNT GILQA+Q+NRSIG+HFKFPP
Sbjct: 316 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 375

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  +++LSIWIIIYERVFIKMAKKITGKERRLT+KQRITIGI LSI+CMVVSG+VE
Sbjct: 376 GWMNLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIILSIVCMVVSGIVE 435

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           +YRRE+ALKNGSF S  SFAF+LPQHAL+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 436 RYRREAALKNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 495

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSV+S++SSLIVNL+H+V+ +  + PWVGGHDLN+ RLDYYY+ IAI+ TLNLLYFV
Sbjct: 496 FLTLSVASYLSSLIVNLIHSVSGEFAESPWVGGHDLNENRLDYYYFTIAIVGTLNLLYFV 555

Query: 361 FFASRFVTSYDENTKLKREV 381
            FASRFVTSYD   KL  ++
Sbjct: 556 LFASRFVTSYDNKVKLMEDL 575

BLAST of Moc03g31050 vs. ExPASy TrEMBL
Match: A0A6J1E886 (protein NRT1/ PTR FAMILY 2.8 OS=Cucurbita moschata OX=3662 GN=LOC111431644 PE=3 SV=1)

HSP 1 Score: 604.4 bits (1557), Expect = 3.6e-169
Identity = 289/380 (76.05%), Postives = 339/380 (89.21%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTN+SW +GF IPT+CFFFSI  FL GRHTYI+AEPRGS+FSDM RV
Sbjct: 196 VALLIALTGVVYVQTNISWTLGFAIPTMCFFFSITIFLLGRHTYIMAEPRGSMFSDMARV 255

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I +ACRKRR+SVSS+SFYDP MADS   EK+ HT+R+KWLDKAAIIVNPDEELDEQGKPK
Sbjct: 256 IISACRKRRYSVSSYSFYDPSMADSSHEEKLVHTERFKWLDKAAIIVNPDEELDEQGKPK 315

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           NPWRLCSLQQVEG KCLV+IIP+W+SG+GCF+VF+QPNT GILQA+Q+NRSIG+HFKFPP
Sbjct: 316 NPWRLCSLQQVEGFKCLVSIIPIWISGIGCFVVFNQPNTFGILQALQSNRSIGTHFKFPP 375

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  +++LSIWIIIYERVFIKMAKKITGKERRLT+KQRITIGI +SI+CMVVSG+VE
Sbjct: 376 GWMHLAGMISLSIWIIIYERVFIKMAKKITGKERRLTMKQRITIGIVVSIVCMVVSGIVE 435

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           +YRRE+AL+NGSF S  SFAF+LPQHAL+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 436 RYRREAALRNGSFISPISFAFLLPQHALTGLMEAFALVAIMEFFTMHMPEHMRTVAGAIF 495

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLTLSV+S++SSLIVNL+  V+ +  +  WVGGHDLN+ RLDYYY+ IAI+  LNLLYFV
Sbjct: 496 FLTLSVASYLSSLIVNLIQTVSGEFAESAWVGGHDLNENRLDYYYFTIAIVGALNLLYFV 555

Query: 361 FFASRFVTSYDENTKLKREV 381
            FASRFVTSYD   KL  ++
Sbjct: 556 LFASRFVTSYDNKVKLMEDL 575

BLAST of Moc03g31050 vs. ExPASy TrEMBL
Match: A0A5D3BJC6 (Protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G003140 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 2.0e-159
Identity = 283/398 (71.11%), Postives = 331/398 (83.17%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTNVSW +GF IPTICFF SI  FL GRHTYI+ +PRGS+  D+ RV
Sbjct: 191 VALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPRGSMLKDVARV 250

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I AA RKR HS+SS SFYD PM DS  GEK+ HTDR+KWLD+AAIIVNP+EELDEQGKPK
Sbjct: 251 IVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPEEELDEQGKPK 310

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           N WRLCSLQQVEG KCLV+I+PVW+SG+GCFIVF+QPNT GILQAMQ+NRSIGSHFKFPP
Sbjct: 311 NSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQAMQSNRSIGSHFKFPP 370

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  ++ALSIWIIIYERV IK+ KK+TGKERRLT++QRITIGI LSI+ M+ SGVVE
Sbjct: 371 GWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIGIVLSILSMITSGVVE 430

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           K+RR++ALKN  F S TSFA +LPQH L+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 431 KHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFTMHMPEHMRTVAGAIF 490

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLT+SV+S++SSLIV+++  V+ K  K PWVGGHDLN  RLDYYY+ IA+I TLNLLYFV
Sbjct: 491 FLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYYFTIAVIETLNLLYFV 550

Query: 361 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKE 399
           FFA RFV  YD+  KL         TEN  + DLP K+
Sbjct: 551 FFARRFVRGYDDKVKL---------TENGRRNDLPVKD 579

BLAST of Moc03g31050 vs. ExPASy TrEMBL
Match: A0A1S3AU85 (protein NRT1/ PTR FAMILY 2.8 OS=Cucumis melo OX=3656 GN=LOC103483003 PE=3 SV=1)

HSP 1 Score: 572.0 bits (1473), Expect = 2.0e-159
Identity = 283/398 (71.11%), Postives = 331/398 (83.17%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +ALLIALTGVVYVQTNVSW +GF IPTICFF SI  FL GRHTYI+ +PRGS+  D+ RV
Sbjct: 191 VALLIALTGVVYVQTNVSWTLGFAIPTICFFISISIFLLGRHTYIIVKPRGSMLKDVARV 250

Query: 61  ITAACRKRRHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPK 120
           I AA RKR HS+SS SFYD PM DS  GEK+ HTDR+KWLD+AAIIVNP+EELDEQGKPK
Sbjct: 251 IVAAYRKRGHSISSSSFYDSPMEDSTCGEKLIHTDRFKWLDRAAIIVNPEEELDEQGKPK 310

Query: 121 NPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSHFKFPP 180
           N WRLCSLQQVEG KCLV+I+PVW+SG+GCFIVF+QPNT GILQAMQ+NRSIGSHFKFPP
Sbjct: 311 NSWRLCSLQQVEGCKCLVSILPVWISGIGCFIVFNQPNTFGILQAMQSNRSIGSHFKFPP 370

Query: 181 AWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVE 240
            W+ +  ++ALSIWIIIYERV IK+ KK+TGKERRLT++QRITIGI LSI+ M+ SGVVE
Sbjct: 371 GWMNLAGMIALSIWIIIYERVLIKLGKKMTGKERRLTMEQRITIGIVLSILSMITSGVVE 430

Query: 241 KYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIF 300
           K+RR++ALKN  F S TSFA +LPQH L+GLMEAFA +A+MEFF M+MPEHMRTVAGAIF
Sbjct: 431 KHRRDAALKNKLFISPTSFALLLPQHVLTGLMEAFALVAMMEFFTMHMPEHMRTVAGAIF 490

Query: 301 FLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFV 360
           FLT+SV+S++SSLIV+++  V+ K  K PWVGGHDLN  RLDYYY+ IA+I TLNLLYFV
Sbjct: 491 FLTISVASYLSSLIVDVIKKVSGKIAKSPWVGGHDLNHNRLDYYYFTIAVIETLNLLYFV 550

Query: 361 FFASRFVTSYDENTKLKREVPAEKSTENEHQIDLPEKE 399
           FFA RFV  YD+  KL         TEN  + DLP K+
Sbjct: 551 FFARRFVRGYDDKVKL---------TENGRRNDLPVKD 579

BLAST of Moc03g31050 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 379.0 bits (972), Expect = 4.7e-105
Identity = 198/377 (52.52%), Postives = 270/377 (71.62%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           +AL+IALTGVVY+QTN+SW IGFVIPT C   SI  F+ G+HTYI A+  GS+F+D+V+V
Sbjct: 188 VALVIALTGVVYIQTNISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKV 247

Query: 61  ITAACRKRRHSVSSH-SFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKP 120
           +TAAC+KR+    S  +FY  P  D      +    R ++ DKA+I+ NP+ EL+E G  
Sbjct: 248 VTAACKKRKVKPGSDITFYIGPSNDGSPTTLVRDKHRLRFFDKASIVTNPN-ELNEDGNA 307

Query: 121 KNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSH-FKF 180
           K  WRLCS+QQV+ LKC+ AI+PVWV+G+ CFI+ DQ N  GILQAMQ +++ G H F+ 
Sbjct: 308 KYKWRLCSVQQVKNLKCVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQV 367

Query: 181 PPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGV 240
           P  W+ + S++ L+IWI +YE V I + K+ITG+++RLT+K RI   I + I+CM+V+G 
Sbjct: 368 PAGWMNLVSMITLAIWISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGF 427

Query: 241 VEKYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGA 300
            EK RR SALKNGSF S  S   +LPQ AL+GL EAF+ +A+MEF  + MPEHMR VAGA
Sbjct: 428 QEKKRRASALKNGSFVSPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGA 487

Query: 301 IFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLY 360
           IFFL+ S++S++ +L++N++ AVT K GK  W+G  DLN+ RL+ Y+++IA I   NLLY
Sbjct: 488 IFFLSSSIASYICTLLINVIDAVTRKEGK-SWLGDKDLNKNRLENYFFIIAGIQVANLLY 547

Query: 361 FVFFASRFVTSYDENTK 376
           F  FASR+ T   EN K
Sbjct: 548 FRLFASRYAT---ENKK 557

BLAST of Moc03g31050 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 255.0 bits (650), Expect = 1.0e-67
Identity = 139/370 (37.57%), Postives = 226/370 (61.08%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           + L+I  T VVY+Q  VSW IGF IPT     +++ F  G   Y+  +P GSIFS + +V
Sbjct: 223 VVLIITQTVVVYIQDQVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQV 282

Query: 61  ITAACRKRRHSVSSH-----SFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDE 120
           I AA +KR+  + +      ++YDP +  S+   K+  +++++ LDKAA+++  D  L  
Sbjct: 283 IVAARKKRKLKLPAEDDGTVTYYDPAIKSSV-LSKLHRSNQFRCLDKAAVVIEGD--LTP 342

Query: 121 QGKPKNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSH 180
           +G P + WRLCS+Q+VE +KCL+ I+P+W +G+          T  + QA++ +R++G  
Sbjct: 343 EGPPADKWRLCSVQEVEEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPK 402

Query: 181 FKFPPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVV 240
           F+ P   + + SLL + I++  Y+RVF+   ++ITG +  +T+ QRI  GI  +I  M+V
Sbjct: 403 FEIPAGSLSVISLLTIGIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIV 462

Query: 241 SGVVEKYRRESALKNGSFNSTT--SFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMR 300
           +G+VE+ RR  ++  G     T  S  ++ PQ  L GL EAF  I  +EFFN   PEHMR
Sbjct: 463 AGIVERMRRIRSINAGDPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMR 522

Query: 301 TVAGAIFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIAT 360
           ++A ++F L+ + SS++SS +V +VH  +    +  W+   +LN  +LDY+YYLIA++  
Sbjct: 523 SIANSLFSLSFAGSSYLSSFLVTVVHKFSGGHDRPDWL-NKNLNAGKLDYFYYLIAVLGV 582

Query: 361 LNLLYFVFFA 364
           +NL+YF + A
Sbjct: 583 VNLVYFWYCA 588

BLAST of Moc03g31050 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 246.5 bits (628), Expect = 3.6e-65
Identity = 141/371 (38.01%), Postives = 225/371 (60.65%), Query Frame = 0

Query: 1   MALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRV 60
           M L+ + T VVY+QT VSW IGF IPT     +++ F  G   Y+  +P GS+FS + RV
Sbjct: 186 MVLIFSHTVVVYLQT-VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARV 245

Query: 61  ITAACRKRRHSVS-----SHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDE 120
           I AA +KR   +S     +  +Y+PP+   +   K+  TD++K+LDKAA+I+  D +L  
Sbjct: 246 IVAARKKRDLKISLVDDGTEEYYEPPVKPGV-LSKLPLTDQFKFLDKAAVIL--DGDLTS 305

Query: 121 QGKPKNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGSH 180
           +G P N WRLCS+Q+VE +KCL+ ++PVW +G+   +      T  + QA + +R +G H
Sbjct: 306 EGVPANKWRLCSIQEVEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPH 365

Query: 181 FKFPPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVV 240
           F+ P A + + S + + IW+ IYE + +    ++  ++ R+T+ QR+ IGI  +I+ M  
Sbjct: 366 FEIPAASITVISYITIGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFT 425

Query: 241 SGVVEKYRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTV 300
           +G VE  RR  A +       + F   LP   L GL E+F  I ++EFFN   PEHMR++
Sbjct: 426 AGFVEGVRRTRATE---MTQMSVFWLALPL-ILMGLCESFNFIGLIEFFNSQFPEHMRSI 485

Query: 301 AGAIFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLN 360
           A ++F L+ + ++++SSL+V  VH V+       W+   DL++ +LDY+YYLIA++  +N
Sbjct: 486 ANSLFPLSFAAANYLSSLLVTTVHKVSGTKDHPDWL-NKDLDRGKLDYFYYLIAVLGVVN 545

Query: 361 LLYFVFFASRF 367
           L+YF + A R+
Sbjct: 546 LVYFWYCAHRY 545

BLAST of Moc03g31050 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 245.0 bits (624), Expect = 1.1e-64
Identity = 140/401 (34.91%), Postives = 238/401 (59.35%), Query Frame = 0

Query: 2   ALLIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRVI 61
           A +++LT VVYVQ+NVSW IG  IP +  F + L F  G   Y+  +  GS  + + +VI
Sbjct: 216 AQILSLTLVVYVQSNVSWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVI 275

Query: 62  TAACRKR------RHSVSSHSFYDPPMADSLDGEKMAHTDRYKWLDKAAIIVNPDEELDE 121
             A +KR      +  ++ +++Y P  A+S    K+ +TD++++LDKAAI+  P+++L  
Sbjct: 276 AVAIKKRGLKPAKQPWLNLYNYYPPKYANS----KLKYTDQFRFLDKAAIL-TPEDKLQP 335

Query: 122 QGKPKNPWRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSIGS- 181
            GKP +PW+LC++QQVE +KC+V ++P+W +    ++   Q  T  + QA+Q++R +GS 
Sbjct: 336 DGKPADPWKLCTMQQVEEVKCIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSG 395

Query: 182 HFKFPPAWVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMV 241
            F  P A   +  +  ++++I++Y+RV +   ++ITG +  +T+ QRI  GIF +   +V
Sbjct: 396 GFVIPAATYVVFLMTGMTVFIVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLV 455

Query: 242 VSGVVEKYRRESALKNGSFN--------STTSFAFMLPQHALSGLMEAFATIAVMEFFNM 301
           V+G VE+ RR  AL   +          S+ S  +++PQ +L+G+ EAFA I  MEF+  
Sbjct: 456 VAGFVEERRRTFALTKPTLGMAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYK 515

Query: 302 NMPEHMRTVAGAIFFLTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYY 361
             PE+MR+ AG+IF++   VSS++ S ++  VH  T  +    W+   DLN+ RLD +Y+
Sbjct: 516 QFPENMRSFAGSIFYVGGGVSSYLGSFLIATVHRTTQNSSGGNWL-AEDLNKGRLDLFYF 575

Query: 362 LIAIIATLNLLYFVFFASRF--------VTSYDENTKLKRE 380
           +IA I  +N  YF+  +  +        VT+Y+ N  + ++
Sbjct: 576 MIAGILAVNFAYFLVMSRWYRYKGSDDEVTTYETNENIIKQ 610

BLAST of Moc03g31050 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.2e-63
Identity = 128/365 (35.07%), Postives = 232/365 (63.56%), Query Frame = 0

Query: 4   LIALTGVVYVQTNVSWAIGFVIPTICFFFSILFFLWGRHTYILAEPRGSIFSDMVRVITA 63
           L+++T V+YVQ N+SW IGF IPT+  FF++L    G   Y+  +P GS+FS + +V+ A
Sbjct: 194 LVSMTLVLYVQNNISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVFKVLVA 253

Query: 64  ACRKRRHSVSSHSFYDPPMADS-LDGEKMAHTDRYKWLDKAAIIVNPDEELDEQGKPKNP 123
           A +KR+   +S   Y  P+ ++ L   K+  TD++++L+KA I++N DE  +E+      
Sbjct: 254 AYKKRKARFTSGIDYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNNDEAGNEE------ 313

Query: 124 WRLCSLQQVEGLKCLVAIIPVWVSGMGCFIVFDQPNTLGILQAMQTNRSI-GSHFKFPPA 183
           WR C+++Q+E +K +++IIP++ S +  F+  +Q  T  + QA++ +    G+ +  PPA
Sbjct: 314 WRTCTVRQIEDIKSIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTSYLIPPA 373

Query: 184 WVKMGSLLALSIWIIIYERVFIKMAKKITGKERRLTVKQRITIGIFLSIMCMVVSGVVEK 243
            + + SLL + IW+  YE V ++  + IT +   +++ Q++ IG   SI  M++SG+VE+
Sbjct: 374 SITVISLLNIGIWLPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLISGIVER 433

Query: 244 YRRESALKNGSFNSTTSFAFMLPQHALSGLMEAFATIAVMEFFNMNMPEHMRTVAGAIFF 303
            RR+ +L NG      S  ++ PQ  L G  + F  + + EFFN  +P +MR++  ++ +
Sbjct: 434 KRRDLSL-NG---VKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSIGNSLLY 493

Query: 304 LTLSVSSFVSSLIVNLVHAVTSKTGKIPWVGGHDLNQTRLDYYYYLIAIIATLNLLYFVF 363
           L LS++S++SS +V++VH+VT++ G+  W+   D+++++LD +YY IA ++TLN ++F +
Sbjct: 494 LGLSLASYLSSAMVSIVHSVTARGGQ-SWL-TDDIDKSKLDCFYYFIAALSTLNFIFFFW 546

Query: 364 FASRF 367
            A R+
Sbjct: 554 CARRY 546