Moc03g30380 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g30380
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	chr3: 21643436 .. 21645326 (+)
RNA-Seq Expression	Moc03g30380
Synteny	Moc03g30380

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGAGACTCTAAGGTATTTGGCTGGAATTGCAGGGCAAAGTGGGTATGGCTCTAAATCCACTGCAGAGCAAGTTACTCAACATTCTCACTTTTCTTCTCCTTTGACTGCAATTATAACTGGTTAGTTACTGTTTCCAAATGTTTTATAAATACCACTGTTTTTAACATCTCTAACTTATCATCTCTTGTATTGGGTTTTGTTTTAATTCACTTTGTTCTGCAAGTTGCATGCTTTAATTTTATGGTTTAGTTTGTGATTTGTACTAAACAGAAATGTATTGAAAACCAAAGTTTACCAAGTTGAACATATGCATGTGTGTGTGTAGTGGAATTGAACCAAATGCCAACCAAATTAATCATATCATAGACTGCCATATACATGGAACATAAGAATACATATTGAAGGAAACAAGGTGAAATCGCAGCATCATCAATCAAACCATATAATGTTGCCAAAGAATGATATCAAAAGAAGACAGAAGCAATCAAAATACAGCTCTGAAAAGTTAAAGTCCAAGAATGATAACTAAATGAAGTTAACAAAGGTTGATCTTTTATTGAGTATGAACTAACAAGAAATGTGTGGTGGTGGAAACTGAAAGGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTGATGGCAAAGAGAGGGGTGAGAATAGTGATAGCAGCGAGAGACCTGAAGAAGGCAGCAAAAGTGAAAGAGAGGATCAAAAGAGAAAGTGCAGAAGCAGAAATTTTAGTGTCAGAGATAGACCTCAGCTCCTTGGCTTCTGTGAGGAGATTCTGTGTGGACTTTGCAGCTTCAGGGCTACCACTCAACATTCTCATGTGAGTTTGCCTTCACTTTTCCCAACCAAAAACCCCCATTTTTCCTCACCCCCTTGTCGTCTTGCTTTTCGCAGAAACAATGCTGGAGTTTTCTCTCCAAATTTGGAGTATTCCGACGACAAGTTTGAATTGACATTTGCTACTAATTATTTGGGTAATTTTCACTTCTCAGCTAAACAAATACAAATAGATAGCGAAAGGAAAAGAGGAACAGTAATTTTCCCTTTTCTCCCGCAGGGCATTACCTTCTGACAGAGATGCTTTTAGACAATTTGATAGAAACATCAAAGAGAACAGGAATCCAAGGAAGAATAATAAACGTCTCTTCTGTTGTCCACTGTTGGGTGAAGAAAGATGGCCTCTGCTTCACTCACCTGCTCAACCCAAACAAGTTAGAAAATTTGCTACATGGTTTCTGCTCAATAATCAGCCAATGTATGGTAGAGAAATTAAAGCAAATGCAAAACTAAACCATGTGCTTGTGCAGATACAATGCCACGCTTGCTTATGCTCAGTCAAAACTAGCTTGCATTTTACATGCCAAAGAGGTGGCTAGGCAGTTGAAGGTTATTTTATTATCCATCTTAATCTATCTATCTTTTCTGCAAACAAACAGCCCATGTTCTGAATTTCACTCATTTTTACAGGAAAGAGCAGCCAATGTAACAGTTAATGCACTGCATCCAGGAATTGTAAAGACTGCAATTATCAGAGCCCATGGAGGATTTATCACAGGCATGAACTATTGTGTACATTTTGCCTAAAGCAAAATAACAAAGAGGGTATTGAATTATTATAATTATTCTAACATGTTCTGCAGATTCTCTGTTCTTCATGGCATCAAAGTTGCTGAAGTCTATTCCTCAGGGTGCGGCTACAACATGCTATGTGGCTCTAAACCCACAAACAGAGAGAAAAACAGGGAAATATTTTTCAGATTGTAATGAGACCAAGTGTTCAGCTCTAGCCAATAATGAGTTAGCAGCACGAAAGCTGTGGATGCAAACTCGTGCCCTTATTCACAGGCAACTTGGTGAAACAGCTTCAGCTTAA

mRNA sequence

ATGAAGGAGACTCTAAGGTATTTGGCTGGAATTGCAGGGCAAAGTGGGTATGGCTCTAAATCCACTGCAGAGCAAGTTACTCAACATTCTCACTTTTCTTCTCCTTTGACTGCAATTATAACTGGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTGATGGCAAAGAGAGGGGTGAGAATAGTGATAGCAGCGAGAGACCTGAAGAAGGCAGCAAAAGTGAAAGAGAGGATCAAAAGAGAAAGTGCAGAAGCAGAAATTTTAGTGTCAGAGATAGACCTCAGCTCCTTGGCTTCTGTGAGGAGATTCTGTGTGGACTTTGCAGCTTCAGGGCTACCACTCAACATTCTCATAAACAATGCTGGAGTTTTCTCTCCAAATTTGGAGTATTCCGACGACAAGTTTGAATTGACATTTGCTACTAATTATTTGGGGCATTACCTTCTGACAGAGATGCTTTTAGACAATTTGATAGAAACATCAAAGAGAACAGGAATCCAAGGAAGAATAATAAACGTCTCTTCTGTTGTCCACTGTTGGGTGAAGAAAGATGGCCTCTGCTTCACTCACCTGCTCAACCCAAACAAATACAATGCCACGCTTGCTTATGCTCAGTCAAAACTAGCTTGCATTTTACATGCCAAAGAGGTGGCTAGGCAGTTGAAGGAAAGAGCAGCCAATGTAACAGTTAATGCACTGCATCCAGGAATTGTAAAGACTGCAATTATCAGAGCCCATGGAGGATTTATCACAGATTCTCTGTTCTTCATGGCATCAAAGTTGCTGAAGTCTATTCCTCAGGGTGCGGCTACAACATGCTATGTGGCTCTAAACCCACAAACAGAGAGAAAAACAGGGAAATATTTTTCAGATTGTAATGAGACCAAGTGTTCAGCTCTAGCCAATAATGAGTTAGCAGCACGAAAGCTGTGGATGCAAACTCGTGCCCTTATTCACAGGCAACTTGGTGAAACAGCTTCAGCTTAA

Coding sequence (CDS)

ATGAAGGAGACTCTAAGGTATTTGGCTGGAATTGCAGGGCAAAGTGGGTATGGCTCTAAATCCACTGCAGAGCAAGTTACTCAACATTCTCACTTTTCTTCTCCTTTGACTGCAATTATAACTGGGGCAACATCAGGAATTGGAGAAGAAACAGCAAGAGTGATGGCAAAGAGAGGGGTGAGAATAGTGATAGCAGCGAGAGACCTGAAGAAGGCAGCAAAAGTGAAAGAGAGGATCAAAAGAGAAAGTGCAGAAGCAGAAATTTTAGTGTCAGAGATAGACCTCAGCTCCTTGGCTTCTGTGAGGAGATTCTGTGTGGACTTTGCAGCTTCAGGGCTACCACTCAACATTCTCATAAACAATGCTGGAGTTTTCTCTCCAAATTTGGAGTATTCCGACGACAAGTTTGAATTGACATTTGCTACTAATTATTTGGGGCATTACCTTCTGACAGAGATGCTTTTAGACAATTTGATAGAAACATCAAAGAGAACAGGAATCCAAGGAAGAATAATAAACGTCTCTTCTGTTGTCCACTGTTGGGTGAAGAAAGATGGCCTCTGCTTCACTCACCTGCTCAACCCAAACAAATACAATGCCACGCTTGCTTATGCTCAGTCAAAACTAGCTTGCATTTTACATGCCAAAGAGGTGGCTAGGCAGTTGAAGGAAAGAGCAGCCAATGTAACAGTTAATGCACTGCATCCAGGAATTGTAAAGACTGCAATTATCAGAGCCCATGGAGGATTTATCACAGATTCTCTGTTCTTCATGGCATCAAAGTTGCTGAAGTCTATTCCTCAGGGTGCGGCTACAACATGCTATGTGGCTCTAAACCCACAAACAGAGAGAAAAACAGGGAAATATTTTTCAGATTGTAATGAGACCAAGTGTTCAGCTCTAGCCAATAATGAGTTAGCAGCACGAAAGCTGTGGATGCAAACTCGTGCCCTTATTCACAGGCAACTTGGTGAAACAGCTTCAGCTTAA

Protein sequence

MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGVRIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILINNAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHCWVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVKTAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSALANNELAARKLWMQTRALIHRQLGETASA

Homology

[Close]

BLAST of Moc03g30380 vs. NCBI nr
Match: XP_022137126.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Momordica charantia])

HSP 1 Score: 637.1 bits (1642), Expect = 8.3e-179
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA
Sbjct: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300

Query: 301 LANNELAARKLWMQTRALIHRQLGETASA 330
           LANNELAARKLWMQTRALIHRQLGETASA
Sbjct: 301 LANNELAARKLWMQTRALIHRQLGETASA 329

[Close]

BLAST of Moc03g30380 vs. NCBI nr
Match: XP_023000696.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 563.5 bits (1451), Expect = 1.2e-156
Identity = 287/325 (88.31%), Postives = 307/325 (94.46%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYL GIAG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIV+AARD+KKAAKV+ERIKRE  EAEILVSEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LLD LIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKD LCFTHLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAII+AH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQT+RKTG+YF+DCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

Query: 301 LANNELAARKLWMQTRALIHRQLGE 326
           LAN+ELAAR LWMQTRALI R+LGE
Sbjct: 314 LANDELAARTLWMQTRALILRELGE 338

[Close]

BLAST of Moc03g30380 vs. NCBI nr
Match: XP_023519347.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 559.7 bits (1441), Expect = 1.7e-155
Identity = 284/325 (87.38%), Postives = 306/325 (94.15%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYL GIAG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRG+
Sbjct: 1   MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGM 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIV+AARD+KKAAKVKERIKRE  EAEILVSEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHY++TE+LLD LIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NGGVFSPNLEYSDDKLELTFATNYLGHYIMTELLLDKLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKD LCFTHLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 181 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 240

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAI++AH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQT RKTG+YF+DCNETKCS+
Sbjct: 241 TAILKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTRRKTGRYFADCNETKCSS 300

Query: 301 LANNELAARKLWMQTRALIHRQLGE 326
           LAN+ELAARKLW+QTRALI R+L E
Sbjct: 301 LANDELAARKLWIQTRALILRELDE 325

[Close]

BLAST of Moc03g30380 vs. NCBI nr
Match: XP_022924073.1 (short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 556.2 bits (1432), Expect = 1.9e-154
Identity = 283/325 (87.08%), Postives = 305/325 (93.85%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYL G+AG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIV+AARD+KKAAKV+ERIKR   EAEIL+SEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LLD LIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKD LCFTHLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAII+AH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQT RKTG+YF+DCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

Query: 301 LANNELAARKLWMQTRALIHRQLGE 326
           LAN+ELAAR LW+QTRALI R+LGE
Sbjct: 314 LANDELAARTLWIQTRALILRELGE 338

[Close]

BLAST of Moc03g30380 vs. NCBI nr
Match: XP_038895224.1 (short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa hispida])

HSP 1 Score: 552.7 bits (1423), Expect = 2.1e-153
Identity = 283/327 (86.54%), Postives = 306/327 (93.58%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYL GIAG SGYGSK++AE+VT+   FSSP TAIITGATSGIGEETARV+AKRGV
Sbjct: 1   MKETLRYLVGIAGPSGYGSKASAEKVTESFQFSSPFTAIITGATSGIGEETARVLAKRGV 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIV+AARD+KKAAKVKERIKRES EAEILVSEIDLSSLASVRRFCV+FAA GLPLNILIN
Sbjct: 61  RIVMAARDMKKAAKVKERIKRESPEAEILVSEIDLSSLASVRRFCVEFAALGLPLNILIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYS+DKFELTFATNYLGHYLLTE+LLD LIETSKRTGIQGRIINVSSVVH 
Sbjct: 121 NGGVFSPNLEYSEDKFELTFATNYLGHYLLTELLLDKLIETSKRTGIQGRIINVSSVVHY 180

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVK++ LCF HLLNPNKYN T AYAQSKLACILHAKEVAR+LKE+ ANVT+NA+HPGIVK
Sbjct: 181 WVKREDLCFIHLLNPNKYNGTRAYAQSKLACILHAKEVARKLKEQDANVTINAVHPGIVK 240

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAIIRAH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQT+RKTGKYF+DCN+TKCS+
Sbjct: 241 TAIIRAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGKYFADCNKTKCSS 300

Query: 301 LANNELAARKLWMQTRALIHRQLGETA 328
           LAN+EL AR LWMQTRALI R+LGE A
Sbjct: 301 LANDELTARTLWMQTRALILRELGEPA 327

[Close]

BLAST of Moc03g30380 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 272.3 bits (695), Expect = 7.1e-72
Identity = 148/319 (46.39%), Postives = 213/319 (66.77%), Query Frame = 0


Query: 10  GIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGVRIVIAARDL 69
           G  G SG+ S+STAE+VT H    + LTAI+TGA+SGIG ETARV++ RGV +V+A R+ 
Sbjct: 5   GSKGASGFSSRSTAEEVT-HGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVRNT 64

Query: 70  KKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILINNAGVFSPNL 129
              AKVKE I ++   A++ V E+DLSS+ SVR+F  ++ ++GLPLN+LINNAG+ +   
Sbjct: 65  DSGAKVKEDIVKQVPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGIMACPF 124

Query: 130 EYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHCWVKKDGLCF 189
             S D  EL FATN+LGH+LLT++LLD +  TS+ +  +GRI+N+SS  H +   +G+ F
Sbjct: 125 MLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYPEGVRF 184

Query: 190 THLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVKTAIIRAHGG 249
             + + + Y++  AY QSKL  +LHA E+ +QLKE   N+T N+LHPG + T + R    
Sbjct: 185 DKINDKSSYSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIMTNLGRYFNP 244

Query: 250 FITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSALANNELAAR 309
           ++  ++  +A  +LKS+PQGAATTCYVALNPQ    +G+YF D N  K   L  +   A+
Sbjct: 245 YLAVAVGAVAKYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKPLPLVKDTELAK 304

Query: 310 KLWMQTRALIHRQLGETAS 329
           K+W  +  L   Q GE++S
Sbjct: 305 KVWDFSTKLTDSQSGESSS 322

[Close]

BLAST of Moc03g30380 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 271.9 bits (694), Expect = 9.2e-72
Identity = 149/305 (48.85%), Postives = 204/305 (66.89%), Query Frame = 0


Query: 8   LAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGVRIVIAAR 67
           + G AG+SGYGS STAE VT HS  +  LTAIITG TSGIG E ARV+  RG  ++IAAR
Sbjct: 7   VTGKAGKSGYGSASTAEDVT-HSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIAAR 66

Query: 68  DLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILINNAGVFSP 127
           + K A   KE I +    A I   ++DLSS+ SVR F   F A  +PLNILINNAGV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHCWVKKDGL 187
             + S+D  E  FATN++GH+LLT +LLD +  +++ +GI+GRI+N+SS+ H +   +G+
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 CFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVKTAIIRAH 247
            F ++ +P++Y+   AY QSKLA +LH+  ++R+L+E   N+T+N++HPG++ T + R H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 248 GGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSALANNELA 307
            G     L  M+  L K+IPQGAATTCYVAL+P  +  TGKYF+DCN T  S  A +   
Sbjct: 247 SGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDCNVTTPSNFAIDTTL 306

Query: 308 ARKLW 313
           A KLW
Sbjct: 307 ADKLW 309

[Close]

BLAST of Moc03g30380 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-71
Identity = 148/305 (48.52%), Postives = 204/305 (66.89%), Query Frame = 0


Query: 8   LAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGVRIVIAAR 67
           + G AG+SGYGS STAE VT HS  +  LTAIITG TSGIG E ARV+  RG  ++IA+R
Sbjct: 7   VTGKAGKSGYGSASTAEDVT-HSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHVIIASR 66

Query: 68  DLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILINNAGVFSP 127
           + K A   KE I +    A I   ++DLSS+ SVR F   F A  +PLNILINNAGV   
Sbjct: 67  NTKAANDSKEMILQMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILINNAGVMFC 126

Query: 128 NLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHCWVKKDGL 187
             + S+D  E  FATN++GH+LLT +LLD +  +++ +GI+GRI+N+SS+ H +   +G+
Sbjct: 127 PFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAHTYTYTEGI 186

Query: 188 CFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVKTAIIRAH 247
            F ++ +P++Y+   AY QSKLA +LH+  ++R+L+E   N+T+N++HPG++ T + R H
Sbjct: 187 MFDYINDPDRYSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVHPGLITTNLFR-H 246

Query: 248 GGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSALANNELA 307
            G     L  M+  L K+IPQGAATTCYVAL+P  +  TGKYF+DCN T  S  A +   
Sbjct: 247 SGLGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADCNVTTPSNFAIDTTL 306

Query: 308 ARKLW 313
           A KLW
Sbjct: 307 ADKLW 309

[Close]

BLAST of Moc03g30380 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 2.9e-65
Identity = 134/300 (44.67%), Postives = 197/300 (65.67%), Query Frame = 0


Query: 13  GQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGVRIVIAARDLKKA 72
           G SG+   STAEQVT H   ++ LTAI+TGA+SGIG ET RV+A RG  +++  R++  A
Sbjct: 9   GVSGFSGSSTAEQVT-HGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAA 68

Query: 73  AKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILINNAGVFSPNLEYS 132
             VK+ I ++   A++   E+DLSSL SV++F  +F +SG PLNILINNAG+ +   + S
Sbjct: 69  KDVKDTILKDIPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFKLS 128

Query: 133 DDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHCWVKKDGLCFTHL 192
            D  EL FATN++GH+LLT +LLD + +T++ +  +GRI+NV+S  H +   +G+ F  +
Sbjct: 129 KDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFDKI 188

Query: 193 LNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVKTAIIRAHGGFIT 252
            + + YN   AY QSKLA +LHA ++ + LKE   N+T N+LHPG + T + R H   + 
Sbjct: 189 NDQSSYNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFR-HNSAVN 248

Query: 253 DSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSALANNELAARKLW 312
             +  +   +LK++ QGAATTCYVAL+PQ +  +G+YFSD N  K +    +   A+KLW
Sbjct: 249 GLINVIGKLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTPHGKDVDLAKKLW 306

[Close]

BLAST of Moc03g30380 vs. ExPASy Swiss-Prot
Match: Q8TC12 (Retinol dehydrogenase 11 OS=Homo sapiens OX=9606 GN=RDH11 PE=1 SV=2)

HSP 1 Score: 177.9 bits (450), Expect = 1.8e-43
Identity = 109/274 (39.78%), Postives = 165/274 (60.22%), Query Frame = 0


Query: 39  IITGATSGIGEETARVMAKRGVRIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSL 98
           ++TGA +GIG+ETA+ +A+RG R+ +A RD++K   V + I+  +   ++LV ++DLS  
Sbjct: 45  VVTGANTGIGKETAKELAQRGARVYLACRDVEKGELVAKEIQTTTGNQQVLVRKLDLSDT 104

Query: 99  ASVRRFCVDFAASGLPLNILINNAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNL 158
            S+R F   F A    L++LINNAGV       + D FE+    N+LGH+LLT +LL+ L
Sbjct: 105 KSIRAFAKGFLAEEKHLHVLINNAGVMMCPYSKTADGFEMHIGVNHLGHFLLTHLLLEKL 164

Query: 159 IETSKRTGIQGRIINVSSVVHCWVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEV 218
            E++       RI+NVSS+ H       + F +L     YNA LAY  SKLA IL  +E+
Sbjct: 165 KESA-----PSRIVNVSSLAH---HLGRIHFHNLQGEKFYNAGLAYCHSKLANILFTQEL 224

Query: 219 ARQLKERAANVTVNALHPGIVKTAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVAL 278
           AR+LK   + VT  ++HPG V++ ++R H  F+   ++++ S  +K+  QGA T+ + AL
Sbjct: 225 ARRLK--GSGVTTYSVHPGTVQSELVR-HSSFMR-WMWWLFSFFIKTPQQGAQTSLHCAL 284

Query: 279 NPQTERKTGKYFSDCNETKCSALANNELAARKLW 313
               E  +G +FSDC+    SA A NE  AR+LW
Sbjct: 285 TEGLEILSGNHFSDCHVAWVSAQARNETIARRLW 306

[Close]

BLAST of Moc03g30380 vs. ExPASy TrEMBL
Match: A0A6J1C5L3 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111008680 PE=3 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 4.0e-179
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV
Sbjct: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN
Sbjct: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC
Sbjct: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK
Sbjct: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA
Sbjct: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300

Query: 301 LANNELAARKLWMQTRALIHRQLGETASA 330
           LANNELAARKLWMQTRALIHRQLGETASA
Sbjct: 301 LANNELAARKLWMQTRALIHRQLGETASA 329

[Close]

BLAST of Moc03g30380 vs. ExPASy TrEMBL
Match: A0A6J1KGJ5 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495058 PE=3 SV=1)

HSP 1 Score: 563.5 bits (1451), Expect = 5.7e-157
Identity = 287/325 (88.31%), Postives = 307/325 (94.46%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYL GIAG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGIAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIV+AARD+KKAAKV+ERIKRE  EAEILVSEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKREGPEAEILVSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LLD LIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKD LCFTHLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAII+AH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQT+RKTG+YF+DCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTQRKTGRYFADCNETKCSS 313

Query: 301 LANNELAARKLWMQTRALIHRQLGE 326
           LAN+ELAAR LWMQTRALI R+LGE
Sbjct: 314 LANDELAARTLWMQTRALILRELGE 338

[Close]

BLAST of Moc03g30380 vs. ExPASy TrEMBL
Match: A0A6J1EBB9 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111431615 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 9.1e-155
Identity = 283/325 (87.08%), Postives = 305/325 (93.85%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYL G+AG SGYGSKS+AEQVT+H   SSPLTAIITGATSGIGEETARV+AKRGV
Sbjct: 14  MKETLRYLVGMAGPSGYGSKSSAEQVTEHLQCSSPLTAIITGATSGIGEETARVLAKRGV 73

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           RIV+AARD+KKAAKV+ERIKR   EAEIL+SEIDLSSLASVRRFC++FAA GLPLNILIN
Sbjct: 74  RIVMAARDMKKAAKVRERIKRGGPEAEILMSEIDLSSLASVRRFCMEFAALGLPLNILIN 133

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           N GVFSPNLEYSDDK ELTFATNYLGHYLLTE+LLD LIETS+RTGIQGRIINVSSVVHC
Sbjct: 134 NGGVFSPNLEYSDDKLELTFATNYLGHYLLTELLLDKLIETSRRTGIQGRIINVSSVVHC 193

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVKKD LCFTHLLNPNKYN T AYAQSKLACILHAKEVARQLKER ANVT+NA+HPGIVK
Sbjct: 194 WVKKDDLCFTHLLNPNKYNGTRAYAQSKLACILHAKEVARQLKERDANVTINAVHPGIVK 253

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           TAII+AH GFITDSLFFMASKLLKSIPQGAATTCYVAL+PQT RKTG+YF+DCNETKCS+
Sbjct: 254 TAIIKAHRGFITDSLFFMASKLLKSIPQGAATTCYVALSPQTHRKTGRYFADCNETKCSS 313

Query: 301 LANNELAARKLWMQTRALIHRQLGE 326
           LAN+ELAAR LW+QTRALI R+LGE
Sbjct: 314 LANDELAARTLWIQTRALILRELGE 338

[Close]

BLAST of Moc03g30380 vs. ExPASy TrEMBL
Match: A0A6J1BYZ0 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111006882 PE=3 SV=1)

HSP 1 Score: 480.3 bits (1235), Expect = 6.3e-132
Identity = 251/333 (75.38%), Postives = 287/333 (86.19%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSS-----PLTAIITGATSGIGEETARVM 60
           MKETLRYLAGIAG SGYGS STAEQVTQ+   SS     PLTAIITGATSGIG ETARV+
Sbjct: 15  MKETLRYLAGIAGPSGYGSNSTAEQVTQNYSISSSSTSHPLTAIITGATSGIGAETARVL 74

Query: 61  AKRGVRIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPL 120
           AKRGVRIV+AARDLKKAA+VKE I++E+ EAEI+V EIDLSSLASV+RFC  F A GLPL
Sbjct: 75  AKRGVRIVMAARDLKKAAQVKEGIQKETPEAEIIVCEIDLSSLASVQRFCNQFLALGLPL 134

Query: 121 NILINNAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVS 180
           NILINNAGVFS NLE+S+DK ELTFATNYLGHY LTE LL+ +IET+ +TGI+GRIINVS
Sbjct: 135 NILINNAGVFSKNLEFSEDKVELTFATNYLGHYFLTETLLEKMIETAAKTGIEGRIINVS 194

Query: 181 SVVHCWVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALH 240
           SV+HCWVKKDGL F  +LNPN YN T AYAQSKLA ILHAKE++RQL+ R A VT+NA+H
Sbjct: 195 SVIHCWVKKDGLRFGQMLNPNNYNGTRAYAQSKLANILHAKEMSRQLQARNARVTINAVH 254

Query: 241 PGIVKTAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNE 300
           PGIVKTAIIRAH GFITDSLFFMASKLLK+  QGA+TTCYVAL+ QTE K+GK+++DCNE
Sbjct: 255 PGIVKTAIIRAHKGFITDSLFFMASKLLKTTSQGASTTCYVALSSQTEGKSGKFYADCNE 314

Query: 301 TKCSALANNELAARKLWMQTRALIHRQLGETAS 329
           T CSALA++EL A+KLW +T  LIHR+L + AS
Sbjct: 315 TNCSALASDELEAQKLWTKTHNLIHRRLSKPAS 347

[Close]

BLAST of Moc03g30380 vs. ExPASy TrEMBL
Match: V4TIE0 (Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10021085mg PE=3 SV=1)

HSP 1 Score: 473.0 bits (1216), Expect = 1.0e-129
Identity = 243/323 (75.23%), Postives = 278/323 (86.07%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           MKETLRYLAGIAG SGYGSKSTAEQVTQ S  +S LTAIITGATSGIG ETARV+AKRGV
Sbjct: 1   MKETLRYLAGIAGPSGYGSKSTAEQVTQGS-VASHLTAIITGATSGIGAETARVLAKRGV 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           R+VI ARDLK+AA+VKE I+RES  AE+L+ EIDLSSL SV+RFC  F A GLPLNILIN
Sbjct: 61  RVVIPARDLKRAAEVKEGIQRESPNAEVLLFEIDLSSLVSVQRFCHQFLALGLPLNILIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           NAGV+S NLE+S+DK E+TFATNYLGHYLLTEM+L+ +IET+  TG+QGRIIN+SSV+H 
Sbjct: 121 NAGVYSKNLEFSEDKIEMTFATNYLGHYLLTEMVLEKMIETAAETGVQGRIINLSSVIHS 180

Query: 181 WVKKDGLCFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVK 240
           WVK+D  CFT LLNP  YN T AYAQSKLA I+HAKE++RQLK R A VT+N +HPGIVK
Sbjct: 181 WVKRDDFCFTRLLNPKNYNGTCAYAQSKLATIMHAKEMSRQLKARNARVTINVVHPGIVK 240

Query: 241 TAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSA 300
           T IIRAH GFITDSLFF+ASKLLKSI QGA+TTCY AL+PQ E  +GKYF+DCNE+ CSA
Sbjct: 241 TGIIRAHKGFITDSLFFIASKLLKSISQGASTTCYAALSPQIEGVSGKYFADCNESNCSA 300

Query: 301 LANNELAARKLWMQTRALIHRQL 324
           LAN+E  A+KLW QTRALIHR+L
Sbjct: 301 LANDESEAKKLWKQTRALIHRRL 322

[Close]

BLAST of Moc03g30380 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 5.9e-122
Identity = 228/327 (69.72%), Postives = 273/327 (83.49%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHF-SSPLTAIITGATSGIGEETARVMAKRG 60
           MK TLRYLAGIAG +G+GS+STAEQVTQHS F  S LTAIITG TSGIG ETARV+AKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  VRIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILI 120
           VR+V+A RD+KKA  VKERI RE+ EA+I++ EIDLSSL+SV RFC  F +  LPLNILI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVH 180
           NNAGVFSPNLE+S++K ELTFATN+LGHYLLTEML++ +I+T++++GI+GRIIN+SSV+H
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 CWVKKDGLCFTHLLNP-NKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGI 240
            WVK D   F  LL+P ++YN T AYAQSKLA ILHAK +++QLK+R ANVT+NA+HPGI
Sbjct: 181 NWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 240

Query: 241 VKTAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKC 300
           VKT IIRAH G  TDSLF +ASKLLKSI QGAATTCYVAL+ +T+  +GKYF+DCNET C
Sbjct: 241 VKTGIIRAHKGLFTDSLFLIASKLLKSISQGAATTCYVALSNETKGLSGKYFADCNETNC 300

Query: 301 SALANNELAARKLWMQTRALIHRQLGE 326
           S LAN+E  A KL  Q+RALIH  L E
Sbjct: 301 SDLANDEYVALKLCTQSRALIHDHLHE 327

[Close]

BLAST of Moc03g30380 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 364.0 bits (933), Expect = 1.3e-100
Identity = 191/270 (70.74%), Postives = 229/270 (84.81%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHF-SSPLTAIITGATSGIGEETARVMAKRG 60
           MK TLRYLAGIAG +G+GS+STAEQVTQHS F  S LTAIITG TSGIG ETARV+AKRG
Sbjct: 1   MKGTLRYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRG 60

Query: 61  VRIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILI 120
           VR+V+A RD+KKA  VKERI RE+ EA+I++ EIDLSSL+SV RFC  F +  LPLNILI
Sbjct: 61  VRVVMAVRDMKKAEMVKERIIRENPEADIILFEIDLSSLSSVARFCSQFLSQDLPLNILI 120

Query: 121 NNAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVH 180
           NNAGVFSPNLE+S++K ELTFATN+LGHYLLTEML++ +I+T++++GI+GRIIN+SSV+H
Sbjct: 121 NNAGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIH 180

Query: 181 CWVKKDGLCFTHLLNP-NKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGI 240
            WVK D   F  LL+P ++YN T AYAQSKLA ILHAK +++QLK+R ANVT+NA+HPGI
Sbjct: 181 NWVKPDCFSFPKLLHPISRYNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGI 240

Query: 241 VKTAIIRAHGGFITDSLFFMASKLLKSIPQ 269
           VKT IIRAH G  TDSLF +ASKLLKSI Q
Sbjct: 241 VKTGIIRAHKGLFTDSLFLIASKLLKSISQ 270

[Close]

BLAST of Moc03g30380 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 332.0 bits (850), Expect = 5.3e-91
Identity = 173/321 (53.89%), Postives = 233/321 (72.59%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           M ET +YL G AG SG+GSKSTAE+VT++    S +TA+ITGATSGIG ETARV+AKRG 
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRS-ITAVITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           R++  AR++K A + KERI  E  E EI+V ++DLSS+ASVR F  DF +  LPLN+LIN
Sbjct: 61  RLIFPARNVKAAEEAKERIVSEFPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLLIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           NAG  +     S+D  E+TFATNYLGH+LLT +LL+ +I+T++ TG+QGRI+NV+S +H 
Sbjct: 121 NAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGIHG 180

Query: 181 WVKKDGLCFTHLLNPNK--YNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGI 240
           W   D + +  L++  K  ++AT AYA SKLA +LH KE++ +L++  ANVTVN +HPG+
Sbjct: 181 WFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHPGV 240

Query: 241 VKTAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKC 300
           V+T + R   G +TD +FF+ASKL+K++PQ AATTCYVA NP+    +GKYF+DCNET  
Sbjct: 241 VRTRLTRDREGLLTDLVFFLASKLVKTVPQAAATTCYVATNPRLVNVSGKYFTDCNETTP 300

Query: 301 SALANNELAARKLWMQTRALI 320
           S L  N   A KLW  +  L+
Sbjct: 301 SGLGTNSSEATKLWAASEILV 320

[Close]

BLAST of Moc03g30380 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 326.2 bits (835), Expect = 2.9e-89
Identity = 177/321 (55.14%), Postives = 228/321 (71.03%), Query Frame = 0


Query: 1   MKETLRYLAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGV 60
           M ET+++L G  G SG+GS+STA+ VT +S   S LTAIITGATSGIG ETARV+AKRG 
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRS-LTAIITGATSGIGAETARVLAKRGA 60

Query: 61  RIVIAARDLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILIN 120
           R+V+ AR +K A + K RI  E  +AEI+V  +DLSSL SVRRF  DF +  LPLNILIN
Sbjct: 61  RLVLPARSVKTAEETKARILSEFPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNILIN 120

Query: 121 NAGVFSPNLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHC 180
           NAG ++     S+D  E+TFATNYLGH+LLT++LL  +IET+ +TG+QGRI+NV+SVVH 
Sbjct: 121 NAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVVHS 180

Query: 181 WVKKDGLCFTHLLNPN--KYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGI 240
           W   D L +   ++ N   Y+AT AYA SKLA +LH  E++R L +  ANVT N +HPGI
Sbjct: 181 WFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHPGI 240

Query: 241 VKTAIIRAHGGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKC 300
           VKT + R   G +TD +FF+ SKLLKS+PQ AATTCYVA +P+     GKYFSDCNE + 
Sbjct: 241 VKTRLTRDREGVVTDLVFFLTSKLLKSVPQAAATTCYVATSPRLRNVCGKYFSDCNEARS 300

Query: 301 SALANNELAARKLWMQTRALI 320
           S   +  L A++LW  +  L+
Sbjct: 301 SKSGSCNLKAQRLWTASDLLV 320

[Close]

BLAST of Moc03g30380 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 281.6 bits (719), Expect = 8.3e-76
Identity = 151/305 (49.51%), Postives = 209/305 (68.52%), Query Frame = 0


Query: 8   LAGIAGQSGYGSKSTAEQVTQHSHFSSPLTAIITGATSGIGEETARVMAKRGVRIVIAAR 67
           + G  G SG+GS STAE+VTQ    ++ LTAIITG T GIG ETARV++KRG  +VI AR
Sbjct: 7   ITGRRGPSGFGSASTAEEVTQGID-ATNLTAIITGGTGGIGMETARVLSKRGAHVVIGAR 66

Query: 68  DLKKAAKVKERIKRESAEAEILVSEIDLSSLASVRRFCVDFAASGLPLNILINNAGVFSP 127
           ++  A   K  I R++A A + + ++DLSS+ S++ F  +F A  LPLN+LINNAGV   
Sbjct: 67  NMGAAENAKTEILRQNANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINNAGVMFC 126

Query: 128 NLEYSDDKFELTFATNYLGHYLLTEMLLDNLIETSKRTGIQGRIINVSSVVHCWVKKDGL 187
             + S+D  EL FATN++GH+LLT +LLD +  T+K +G++GRI+NVSSV H +  ++G+
Sbjct: 127 PYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIYTYQEGI 186

Query: 188 CFTHLLNPNKYNATLAYAQSKLACILHAKEVARQLKERAANVTVNALHPGIVKTAIIRAH 247
            F  + +   Y+   AY QSKLA ILHA E++RQL+E   N+T N++HPG++ T + + H
Sbjct: 187 QFDSINDICSYSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPGLILTNLFQ-H 246

Query: 248 GGFITDSLFFMASKLLKSIPQGAATTCYVALNPQTERKTGKYFSDCNETKCSALANNELA 307
              +   L F +  L K+IPQGAATTCYVAL+P  +  TGKYF+DCNE   S LA +E  
Sbjct: 247 TALLMRFLKFFSFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNEVTPSKLARDETL 306

Query: 308 ARKLW 313
           A+KLW
Sbjct: 307 AQKLW 309

The following BLAST results are available for this feature:

BLAST of Moc03g30380 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022137126.1	8.3e-179	100.00	short-chain dehydrogenase TIC 32, chloroplastic-like [Momordica charantia]	[more]
XP_023000696.1	1.2e-156	88.31	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita maxima]	[more]
XP_023519347.1	1.7e-155	87.38	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita pepo subsp. pepo...	[more]
XP_022924073.1	1.9e-154	87.08	short-chain dehydrogenase TIC 32, chloroplastic-like [Cucurbita moschata]	[more]
XP_038895224.1	2.1e-153	86.54	short-chain dehydrogenase TIC 32 A, chloroplastic-like isoform X2 [Benincasa his...	[more]

BLAST of Moc03g30380 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A2RVM0	7.1e-72	46.39	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
A0A078ISJ6	9.2e-72	48.85	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078IS66	2.1e-71	48.52	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
Q6RVV4	2.9e-65	44.67	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q8TC12	1.8e-43	39.78	Retinol dehydrogenase 11 OS=Homo sapiens OX=9606 GN=RDH11 PE=1 SV=2	[more]

BLAST of Moc03g30380 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5L3	4.0e-179	100.00	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
A0A6J1KGJ5	5.7e-157	88.31	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita maxima OX=3661...	[more]
A0A6J1EBB9	9.1e-155	87.08	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucurbita moschata OX=36...	[more]
A0A6J1BYZ0	6.3e-132	75.38	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Momordica charantia OX=3...	[more]
V4TIE0	1.0e-129	75.23	Uncharacterized protein OS=Citrus clementina OX=85681 GN=CICLE_v10021085mg PE=3 ...	[more]

BLAST of Moc03g30380 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50130.1	5.9e-122	69.72	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	1.3e-100	70.74	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT4G24050.1	5.3e-91	53.89	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	2.9e-89	55.14	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	8.3e-76	49.51	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 69..92
None	No IPR available	GENE3D	3.40.50.720		coord: 32..323 e-value: 7.6E-72 score: 243.8
None	No IPR available	PANTHER	PTHR24320:SF198	NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEIN	coord: 4..323
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..323
None	No IPR available	CDD	cd05327	retinol-DH_like_SDR_c_like	coord: 37..312 e-value: 1.34238E-96 score: 284.887
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 204..223 score: 26.08 coord: 169..177 score: 61.15 coord: 113..124 score: 46.66
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 37..54 score: 44.95 coord: 163..179 score: 32.79 coord: 204..223 score: 27.97 coord: 227..244 score: 34.35 coord: 113..124 score: 47.12
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 37..178 e-value: 3.9E-28 score: 98.3
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 37..297

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g30380.1	Moc03g30380.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity