Moc03g29390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g29390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	11-beta-hydroxysteroid dehydrogenase
Location	chr3: 21014408 .. 21015964 (-)
RNA-Seq Expression	Moc03g29390
Synteny	Moc03g29390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCTTATGAACAATTTCTTGAATATCCTTCTTTCTCGTATATCTCTAACGGCCTTCTTCGTCATTCTCCCGGCATACTTGATCTTCAAGTTTCTCTCCTACATAATAAAGTCCATCTTCAGTGAAGATGTCGACGGAAAAGTGGTTCTCATCACCGGAGCTTCCTCAGGCATCGGCGAGGTTCTGCCTCTAGCCCACCCTGTATTTCGACGAATTTTTCACAAATTTGGATTTAACTTATTTTCTTGATTTTTGAAGCATCTGGCGTACGAGTACGCCAAAAGAGGAGCTTATTTAGCGTTGGTTGCCAGAAGGGAAAATCGCCTCCGCGAAGTTGCCCACGTGGCAGAGCTTCTTGGCTCCCCATATGCTCTCGTCATTAAAGCTGATGTTTCCAAGATCGAAGACTGTAAACGCTGTATTGATACAACAATCGCACACTTTGGACGACGTAACTAAATTCTTACAACCCTGATTACAATTACTTCTCAATGTTTTGATTGTTGGTTTGGAAAATATTAGTCCAATCTCTTCTGCCCTGATGGGATTTTGCGAATTTTAATGACGAAACAGTGGATCATCTGGTTAACAATGCCGGGATAACCAGCATCAATTTGTTTGAAGAATATGACGACGTGAGAAATATGGCATCAGTAATGGTGATAATCGCAAAACCCACTTTTCCGATTGAGTGAAATTGAATGCAGTGAGTTTTTAATCCAAATTAAATTTGTGCAGGACATAAACTTCTGGGGAACAGTTTATTGCACTTACAATGCGATTCCACATCTAAAACGAAGCAGAGGGAGGATAATTGGAATTGCTTCCTCCGCGGAATGGCTACCGGCGCCGCGGCTGAGCTTCTACAGTGTAATTAAATTGATATGTTCATGAATATATGAACAGAACATCCCCTGTTTTCATTTTCCATTTCTATTGTGTGCTCTGTTTTTTCTTTTGTGGTGATTGTAGTTCTGTAATAACATTTAAATTGAAGGCAAGCAAAGCAGCAGTGGCAAGTTTCTACGAGACGTTGAGAACGGAAATTGCGAAAGAGGTGGGGATTACGATTGTGACTCCAGGGCTGACGGAATCAGAGGTGACGCAGGGCAAATTCATATCCAAAACAGGGCAGATGTACCTGGATCAAGAATTGAGAGATGTAAGCGAGAATTATTTTGAATTGAATGTAGAATTTTGGTGAGCCATTTTAAACAAATTGTTGGGGGGGGGGGGGGGGGGGGGGGGGGATTGAAGGCTTTAATTAGTGTGATGCCGATTGCGCCGGTGGGAGGCGTGGTGGAAGCAATCGTGAGGAGTGGTTGCAGAGGGGATGAGCACGTGACGGCGCCGGCGTGGATGAGGACGACGTTTTTCTGGAGGGCGTTGGTTCCGGAGGTGGTGGAGTGGATTAATCGCTTGTTTATAATGACTGGAACTTCCCCCACCCACACCTTTGGGCAGAGGCTGCTTCAACTCACTGGATTGAAGACTTTTATTTACCCACCTTCCGTTCTGTCGCCGGCGATCGACTTGGGACAAAAAACCGACTGA

mRNA sequence

ATGGATCTTATGAACAATTTCTTGAATATCCTTCTTTCTCGTATATCTCTAACGGCCTTCTTCGTCATTCTCCCGGCATACTTGATCTTCAAGTTTCTCTCCTACATAATAAAGTCCATCTTCAGTGAAGATGTCGACGGAAAAGTGGTTCTCATCACCGGAGCTTCCTCAGGCATCGGCGAGCATCTGGCGTACGAGTACGCCAAAAGAGGAGCTTATTTAGCGTTGGTTGCCAGAAGGGAAAATCGCCTCCGCGAAGTTGCCCACGTGGCAGAGCTTCTTGGCTCCCCATATGCTCTCGTCATTAAAGCTGATGTTTCCAAGATCGAAGACTGTAAACGCTGTATTGATACAACAATCGCACACTTTGGACGACGACATAAACTTCTGGGGAACAGTTTATTGCACTTACAATGCGATTCCACATCTAAAACGAAGCAGAGGGAGGATAATTGGAATTGCTTCCTCCGCGGAATGGCTACCGGCGCCGCGGCTGAGCTTCTACATTCTGTAATAACATTTAAATTGAAGGCAAGCAAAGCAGCAGTGGCAAGTTTCTACGAGACGTTGAGAACGGAAATTGCGAAAGAGGTGGGGATTACGATTGTGACTCCAGGGCTGACGGAATCAGAGGTGACGCAGGGCAAATTCATATCCAAAACAGGGCAGATGTACCTGGATCAAGAATTGAGAGATGCTTTAATTAGTGTGATGCCGATTGCGCCGGTGGGAGGCGTGGTGGAAGCAATCGTGAGGAGTGGTTGCAGAGGGGATGAGCACGTGACGGCGCCGGCGTGGATGAGGACGACGTTTTTCTGGAGGGCGTTGGTTCCGGAGGTGGTGGAGTGGATTAATCGCTTGTTTATAATGACTGGAACTTCCCCCACCCACACCTTTGGGCAGAGGCTGCTTCAACTCACTGGATTGAAGACTTTTATTTACCCACCTTCCGTTCTGTCGCCGGCGATCGACTTGGGACAAAAAACCGACTGA

Coding sequence (CDS)

ATGGATCTTATGAACAATTTCTTGAATATCCTTCTTTCTCGTATATCTCTAACGGCCTTCTTCGTCATTCTCCCGGCATACTTGATCTTCAAGTTTCTCTCCTACATAATAAAGTCCATCTTCAGTGAAGATGTCGACGGAAAAGTGGTTCTCATCACCGGAGCTTCCTCAGGCATCGGCGAGCATCTGGCGTACGAGTACGCCAAAAGAGGAGCTTATTTAGCGTTGGTTGCCAGAAGGGAAAATCGCCTCCGCGAAGTTGCCCACGTGGCAGAGCTTCTTGGCTCCCCATATGCTCTCGTCATTAAAGCTGATGTTTCCAAGATCGAAGACTGTAAACGCTGTATTGATACAACAATCGCACACTTTGGACGACGACATAAACTTCTGGGGAACAGTTTATTGCACTTACAATGCGATTCCACATCTAAAACGAAGCAGAGGGAGGATAATTGGAATTGCTTCCTCCGCGGAATGGCTACCGGCGCCGCGGCTGAGCTTCTACATTCTGTAATAACATTTAAATTGAAGGCAAGCAAAGCAGCAGTGGCAAGTTTCTACGAGACGTTGAGAACGGAAATTGCGAAAGAGGTGGGGATTACGATTGTGACTCCAGGGCTGACGGAATCAGAGGTGACGCAGGGCAAATTCATATCCAAAACAGGGCAGATGTACCTGGATCAAGAATTGAGAGATGCTTTAATTAGTGTGATGCCGATTGCGCCGGTGGGAGGCGTGGTGGAAGCAATCGTGAGGAGTGGTTGCAGAGGGGATGAGCACGTGACGGCGCCGGCGTGGATGAGGACGACGTTTTTCTGGAGGGCGTTGGTTCCGGAGGTGGTGGAGTGGATTAATCGCTTGTTTATAATGACTGGAACTTCCCCCACCCACACCTTTGGGCAGAGGCTGCTTCAACTCACTGGATTGAAGACTTTTATTTACCCACCTTCCGTTCTGTCGCCGGCGATCGACTTGGGACAAAAAACCGACTGA

Protein sequence

MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIGEHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTIAHFGRRHKLLGNSLLHLQCDSTSKTKQREDNWNCFLRGMATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFISKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPEVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDLGQKTD

Homology

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BLAST of Moc03g29390 vs. NCBI nr
Match: XP_023000976.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita maxima])

HSP 1 Score: 381.7 bits (979), Expect = 6.3e-102
Identity = 205/347 (59.08%), Postives = 254/347 (73.20%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +NN L I++ RIS TAF  I P YL+FKF+ +II+S  +EDV GKVVLITGASSGIG
Sbjct: 1   MDFINNLLKIIIDRISQTAFPFIYPIYLLFKFIFFIIRSTSAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYAKRGAYLALVARR++RLR+VA VAE+LGSPYAL+I ADVS +EDCKRC+DTT+
Sbjct: 61  EHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALLIHADVSNLEDCKRCVDTTV 120

Query: 121 AHFGRRHKLLGNSLL-------HLQCDSTSKTKQREDNWNCF------------LRGMAT 180
           +HFGR   L+ N+ +         +      +    D W                RG   
Sbjct: 121 SHFGRLDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYFAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI  E+GIT+VTPGL ESE+T+GKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MY+DQELRDA+ISVMPI PVGG VEA++R+GCRGDE+VT P W+R +F+W+  +P
Sbjct: 241 FEDGKMYIDQELRDAVISVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDL 326
           EVVEW+N LFIMTG+SPT TFG+ LL+LTGLK  IYPPSV SP + +
Sbjct: 301 EVVEWMNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 346

[Close]

BLAST of Moc03g29390 vs. NCBI nr
Match: KAG6584164.1 (11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 378.6 bits (971), Expect = 5.4e-101
Identity = 203/345 (58.84%), Postives = 251/345 (72.75%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +N+ L + + RIS T F  I P YL+FKF+S+II+S  +EDV GKVVLITGASSGIG
Sbjct: 27  MDFINSILKVTIDRISETVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIG 86

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYAK GAYLALVARR++RLR VA VAE+LGSPYALVI ADVS +EDCKRC+DTT+
Sbjct: 87  EHLAYEYAKTGAYLALVARRDHRLRHVADVAEILGSPYALVIHADVSNLEDCKRCVDTTV 146

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQREDNWNCF------------LRGMATG- 180
           +HFGR   L+ N+      L  + +         D W                RG   G 
Sbjct: 147 SHFGRLDHLVNNAGITSINLFEEYEDPRNIASDTDFWGTVYCTYYAIPYLKQTRGKIIGI 206

Query: 181 --AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFISK 240
             +AA L    ++F   ASKAAV SFYETLR EI  E+GIT+VTPGL ESE+T+GKF+ +
Sbjct: 207 ASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFMFE 266

Query: 241 TGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPEV 300
            G+MY+DQELRDA++SVMPI PVGG VEA++R+GCRGDE+VT P W+R +F+W+  +PEV
Sbjct: 267 DGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLPEV 326

Query: 301 VEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDL 326
           VEW+N LFIMTG+SPT TFG+ LL+LTGLK  IYPPSV SP + +
Sbjct: 327 VEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 370

[Close]

BLAST of Moc03g29390 vs. NCBI nr
Match: XP_008446273.1 (PREDICTED: 11-beta-hydroxysteroid dehydrogenase 1B-like [Cucumis melo])

HSP 1 Score: 377.9 bits (969), Expect = 9.1e-101
Identity = 207/340 (60.88%), Postives = 248/340 (72.94%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MDL+NNFLN+++ RIS TAF  I P Y   K L + I+S F+EDV GKVVLITGASSGIG
Sbjct: 1   MDLINNFLNLIIYRISQTAFSYIFPLYFFVKLLLFPIRSTFAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EH+AYEYAKRGAYLALVARRE+RLREVA VAE+LGSP+ALVI ADVSK++DCKRC+ TT+
Sbjct: 61  EHVAYEYAKRGAYLALVARREHRLREVAEVAEILGSPFALVIHADVSKVDDCKRCVQTTL 120

Query: 121 AHFGRRHKLLGN------SLLHLQCDSTSKTKQRE-DNWNCF------------LRGMAT 180
           AHFGR   L+ N      +L     D  +     + D W                RG   
Sbjct: 121 AHFGRLDHLVNNAGITSINLFEEYDDPRNVASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI ++VGITIVTPGLTESE+TQGKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YTASKAAVISFYETLRVEIGRDVGITIVTPGLTESEMTQGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MYLDQELRDA++SVMPI  VG   EAIV+ GCRGDE+VT P WMR TF+WR  +P
Sbjct: 241 FEDGKMYLDQELRDAVMSVMPIEAVGSAAEAIVKGGCRGDEYVTEPPWMRMTFYWRMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSV 319
           E+VEW+N LFIMTG+SPT +FG+R+L+ TGLK FIYP SV
Sbjct: 301 EMVEWLNHLFIMTGSSPTESFGKRVLEFTGLKPFIYPASV 339

[Close]

BLAST of Moc03g29390 vs. NCBI nr
Match: XP_022923773.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata])

HSP 1 Score: 377.9 bits (969), Expect = 9.1e-101
Identity = 202/347 (58.21%), Postives = 253/347 (72.91%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +N+ L + + RIS T F  I P YL+FKF+S+II+S  +EDV GKVVLITGASSGIG
Sbjct: 1   MDFINSILKVTIDRISQTVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYAKRGAYLALVARR++RLR+VA VAE+LGSPYALVI ADVS +EDCKRC+DTT+
Sbjct: 61  EHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLEDCKRCVDTTV 120

Query: 121 AHFGRRHKLLGNSLL-------HLQCDSTSKTKQREDNWNCF------------LRGMAT 180
           +HFG+   L+ N+ +         +      +    D W                RG   
Sbjct: 121 SHFGQLDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI  E+GIT+VTPGL ESE+T+GKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MY+DQELRDA++SVMPI PVGG VEA++R+GCRGDE+VT P W+R +F+W+  +P
Sbjct: 241 FEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDL 326
           EVVEW+N LFIMTG+SPT TFG+ LL+LTGLK  IYPPSV SP + +
Sbjct: 301 EVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 346

[Close]

BLAST of Moc03g29390 vs. NCBI nr
Match: XP_023519994.1 (11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 377.9 bits (969), Expect = 9.1e-101
Identity = 203/347 (58.50%), Postives = 254/347 (73.20%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +N+ L +++ RIS TAF  I P Y  FKF+S+II+SI +EDV GKVVLITGASSGIG
Sbjct: 1   MDFINSILKVIIDRISQTAFPFIYPIYFFFKFISFIIRSISAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYAKRGAYLALVARR++RLR+VA VAE+LGSPYALVI ADVS ++DC+RC+DTT+
Sbjct: 61  EHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLDDCQRCVDTTV 120

Query: 121 AHFGRRHKLLGN------SLLHLQCDSTSKTKQRE-DNWNCF------------LRGMAT 180
            HFG+   L+ N      +L     D  +     + D W                RG   
Sbjct: 121 GHFGQLDHLVNNAGITSINLFEEYVDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI  E+GIT+VTPGL ESE+T+GKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MY+DQELRDA++SVMPI PVGG VEA++R+GCRGDE+VT P W+R +F+W+  +P
Sbjct: 241 FEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDL 326
           EVVEW+N LFIMTG+SPT TFG+ LL+LTGLK  IYPPSV SP + +
Sbjct: 301 EVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 346

[Close]

BLAST of Moc03g29390 vs. ExPASy Swiss-Prot
Match: P0DKC5 (11-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.0e-67
Identity = 147/346 (42.49%), Postives = 208/346 (60.12%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           M+L+N+FLN+     +       LP +  FKFL  I  +IFSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVSK +DC+R +D TI
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQREDN--WNC---------FLRG-----M 180
            HFGR   L+ N+      +    +  ++TK   D   W           +LR      +
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
           A  ++A  L +       ASKAA+ SF+ET+R E+  +V ITIVTPG  ESE+TQGK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 300
             G++ ++Q++RD  +   P+A   G  ++IV   CR   +VT P+W + T+ W+ L PE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 VVEWINRLFIMTGT--SPTHTFGQRLLQLTGLKTFIYPPSVLSPAI 324
           ++EW  RL  MTGT  S      +R++ + G+++ +YP S+ +P I
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPGVRSTLYPESIRTPEI 346

[Close]

BLAST of Moc03g29390 vs. ExPASy Swiss-Prot
Match: P0DKC6 (11-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.0e-67
Identity = 147/346 (42.49%), Postives = 208/346 (60.12%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           M+L+N+FLN+     +       LP +  FKFL  I  +IFSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVSK +DC+R +D TI
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQREDN--WNC---------FLRG-----M 180
            HFGR   L+ N+      +    +  ++TK   D   W           +LR      +
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
           A  ++A  L +       ASKAA+ SF+ET+R E+  +V ITIVTPG  ESE+TQGK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 300
             G++ ++Q++RD  +   P+A   G  ++IV   CR   +VT P+W + T+ W+ L PE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 VVEWINRLFIMTGT--SPTHTFGQRLLQLTGLKTFIYPPSVLSPAI 324
           ++EW  RL  MTGT  S      +R++ + G+++ +YP S+ +P I
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPGVRSTLYPESIRTPEI 346

[Close]

BLAST of Moc03g29390 vs. ExPASy Swiss-Prot
Match: Q93W57 (11-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 SV=1)

HSP 1 Score: 256.5 bits (654), Expect = 4.0e-67
Identity = 148/344 (43.02%), Postives = 206/344 (59.88%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MDL++ FLN++    +       LP + IFKF   I+ ++FSEDV GKVV+ITGASSGIG
Sbjct: 1   MDLIHTFLNLIAPPFTFFFLLFFLPPFQIFKFFLSILGTLFSEDVAGKVVVITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEYAKRGA L L ARRE  L+EVA  A  LGSP  +V++ADVSK EDC++ +D T+
Sbjct: 61  ESLAYEYAKRGACLVLAARRERSLQEVAERARDLGSPDVVVVRADVSKAEDCRKVVDQTM 120

Query: 121 AHFGRRHKLLGN------SLLHLQCDSTSKTKQREDNW---------------NCFLRGM 180
             FGR   L+ N      S+L    D T   +  + N+               N   R +
Sbjct: 121 NRFGRLDHLVNNAGIMSVSMLEEVEDITGYRETMDINFWGYVYMTRFAAPYLRNSRGRIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
              +++  + +       ASKAA++ F+ETLR E   ++GIT+VTPG  ESE+TQGKF +
Sbjct: 181 VLSSSSSWMPTPRMSFYNASKAAISQFFETLRVEFGPDIGITLVTPGFIESELTQGKFYN 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 300
             G+  +DQ++RD  +S  PI  V     +IVRS  RG+ +VT PAW R T++W+   PE
Sbjct: 241 -AGERVIDQDMRDVQVSTTPILRVESAARSIVRSAIRGERYVTEPAWFRVTYWWKLFCPE 300

Query: 301 VVEWINRLFIMT--GTSPTHTFGQRLLQLTGLKTFIYPPSVLSP 322
           V+EW+ RL  +   G     TFG+++L  TG+K+ +YP +V  P
Sbjct: 301 VMEWVFRLMYLASPGEPEKETFGKKVLDYTGVKSLLYPETVQVP 343

[Close]

BLAST of Moc03g29390 vs. ExPASy Swiss-Prot
Match: A7LB60 (11-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 9.0e-67
Identity = 153/353 (43.34%), Postives = 209/353 (59.21%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MDL++ FLN++    +     + LP Y   KF+  I+  + SE+V GKVV ITGASSGIG
Sbjct: 1   MDLIHTFLNLVAPPFTFFFLCLFLPPYWGLKFMVSILSWLLSENVAGKVVHITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E+LAYEYAKRGA LAL ARRE  L +VA  A  LGSP  +V++ADVSK EDC R ID T+
Sbjct: 61  EYLAYEYAKRGACLALSARRETALHQVADTARHLGSPDVIVMRADVSKPEDCMRLIDQTV 120

Query: 121 AHFGRRHKLLGNSLLHLQC------DSTSKTKQREDN-WNCF------LRGMATGAAAEL 180
            HFGR   L+ N+ + +        D ++     E N W         L+ +       +
Sbjct: 121 NHFGRLDHLVNNAAISIATLFEETPDISNLRPIMETNFWGSVYTTRYALQHLRKSRGKIV 180

Query: 181 LHSVITFKL--------KASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
           + S +   L         ASKAA+ S YETLR E+  E+GITIVTPG  ESE+T+GKF+S
Sbjct: 181 VMSSVDSWLPAPRRHIYSASKAALVSLYETLRVEVGSEIGITIVTPGYIESEITKGKFLS 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 300
             G++ +DQ+LRD  +S +P+  V G  E+I++S  RGD  +T P+W R T+  + L PE
Sbjct: 241 AQGEVDVDQDLRDVEVSAVPVGSVSGCAESIIKSTLRGDRCLTVPSWFRMTYLIKLLCPE 300

Query: 301 VVEWINRLFIMT--GTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDLGQKTD 331
           ++EW  RL  +T  GT  +    +++L  TG K   YPPS+ SP +    KTD
Sbjct: 301 LLEWTFRLLYLTAPGTPTSDALSKKILDATGAKNLFYPPSIQSPDV----KTD 349

[Close]

BLAST of Moc03g29390 vs. ExPASy Swiss-Prot
Match: Q9LUE4 (11-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=HSD6 PE=2 SV=1)

HSP 1 Score: 207.2 bits (526), Expect = 2.8e-52
Identity = 125/311 (40.19%), Postives = 174/311 (55.95%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +N  +N L   ++L A  V  P Y   K    I +++FSE+V GKVV+ITGA+SGIG
Sbjct: 1   MDSINKIINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEY KRGAYLALV  R   L  VA +AEL GSP  L + ADVSK++DC+R I  T+
Sbjct: 61  EALAYEYGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATV 120

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQRED--NWNCFL--------------RGM 180
            HFGR   L+ N+           +  SK     D   W                  R +
Sbjct: 121 LHFGRLDHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
              +    + S       ASKAAV +FYETLRTE   ++G+TIV PG+ +SE+++GKF++
Sbjct: 181 VIASGCGYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMT 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 291
           K G++ +D+ELRD  +SV+P+       +AI+RS CRGD ++  P W+      +    E
Sbjct: 241 KDGKLVVDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSE 300

[Close]

BLAST of Moc03g29390 vs. ExPASy TrEMBL
Match: A0A6J1KHB1 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC111495256 PE=3 SV=1)

HSP 1 Score: 381.7 bits (979), Expect = 3.1e-102
Identity = 205/347 (59.08%), Postives = 254/347 (73.20%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +NN L I++ RIS TAF  I P YL+FKF+ +II+S  +EDV GKVVLITGASSGIG
Sbjct: 1   MDFINNLLKIIIDRISQTAFPFIYPIYLLFKFIFFIIRSTSAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYAKRGAYLALVARR++RLR+VA VAE+LGSPYAL+I ADVS +EDCKRC+DTT+
Sbjct: 61  EHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALLIHADVSNLEDCKRCVDTTV 120

Query: 121 AHFGRRHKLLGNSLL-------HLQCDSTSKTKQREDNWNCF------------LRGMAT 180
           +HFGR   L+ N+ +         +      +    D W                RG   
Sbjct: 121 SHFGRLDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYFAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI  E+GIT+VTPGL ESE+T+GKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MY+DQELRDA+ISVMPI PVGG VEA++R+GCRGDE+VT P W+R +F+W+  +P
Sbjct: 241 FEDGKMYIDQELRDAVISVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDL 326
           EVVEW+N LFIMTG+SPT TFG+ LL+LTGLK  IYPPSV SP + +
Sbjct: 301 EVVEWMNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 346

[Close]

BLAST of Moc03g29390 vs. ExPASy TrEMBL
Match: A0A6J1EAJ7 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111431382 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.4e-101
Identity = 202/347 (58.21%), Postives = 253/347 (72.91%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +N+ L + + RIS T F  I P YL+FKF+S+II+S  +EDV GKVVLITGASSGIG
Sbjct: 1   MDFINSILKVTIDRISQTVFPFIYPIYLLFKFISFIIRSTSAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYAKRGAYLALVARR++RLR+VA VAE+LGSPYALVI ADVS +EDCKRC+DTT+
Sbjct: 61  EHLAYEYAKRGAYLALVARRDHRLRDVADVAEILGSPYALVIHADVSNLEDCKRCVDTTV 120

Query: 121 AHFGRRHKLLGNSLL-------HLQCDSTSKTKQREDNWNCF------------LRGMAT 180
           +HFG+   L+ N+ +         +      +    D W                RG   
Sbjct: 121 SHFGQLDHLVNNAGITSINLFEEYEDPRNIASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI  E+GIT+VTPGL ESE+T+GKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGSEIGITVVTPGLIESEMTKGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MY+DQELRDA++SVMPI PVGG VEA++R+GCRGDE+VT P W+R +F+W+  +P
Sbjct: 241 FEDGKMYIDQELRDAVMSVMPIEPVGGAVEAMLRAGCRGDEYVTEPPWLRMSFYWKMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLSPAIDL 326
           EVVEW+N LFIMTG+SPT TFG+ LL+LTGLK  IYPPSV SP + +
Sbjct: 301 EVVEWLNYLFIMTGSSPTDTFGKWLLELTGLKPLIYPPSVRSPELGI 346

[Close]

BLAST of Moc03g29390 vs. ExPASy TrEMBL
Match: A0A1S3BFC1 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucumis melo OX=3656 GN=LOC103489048 PE=3 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 4.4e-101
Identity = 207/340 (60.88%), Postives = 248/340 (72.94%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MDL+NNFLN+++ RIS TAF  I P Y   K L + I+S F+EDV GKVVLITGASSGIG
Sbjct: 1   MDLINNFLNLIIYRISQTAFSYIFPLYFFVKLLLFPIRSTFAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EH+AYEYAKRGAYLALVARRE+RLREVA VAE+LGSP+ALVI ADVSK++DCKRC+ TT+
Sbjct: 61  EHVAYEYAKRGAYLALVARREHRLREVAEVAEILGSPFALVIHADVSKVDDCKRCVQTTL 120

Query: 121 AHFGRRHKLLGN------SLLHLQCDSTSKTKQRE-DNWNCF------------LRGMAT 180
           AHFGR   L+ N      +L     D  +     + D W                RG   
Sbjct: 121 AHFGRLDHLVNNAGITSINLFEEYDDPRNVASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI ++VGITIVTPGLTESE+TQGKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YTASKAAVISFYETLRVEIGRDVGITIVTPGLTESEMTQGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MYLDQELRDA++SVMPI  VG   EAIV+ GCRGDE+VT P WMR TF+WR  +P
Sbjct: 241 FEDGKMYLDQELRDAVMSVMPIEAVGSAAEAIVKGGCRGDEYVTEPPWMRMTFYWRMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSV 319
           E+VEW+N LFIMTG+SPT +FG+R+L+ TGLK FIYP SV
Sbjct: 301 EMVEWLNHLFIMTGSSPTESFGKRVLEFTGLKPFIYPASV 339

[Close]

BLAST of Moc03g29390 vs. ExPASy TrEMBL
Match: A0A0A0LTX2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G051670 PE=3 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 1.1e-99
Identity = 201/340 (59.12%), Postives = 247/340 (72.65%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MDL+NNFLN+++ RIS TAF  + P Y  FK L + I+S F+EDV GKVVLITGASSGIG
Sbjct: 1   MDLINNFLNLIIDRISQTAFSFVFPLYFFFKLLLFPIRSSFAEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EH+AYEYAKRGAYLALVARRE+RLREVA VAE+LGSP+AL+I ADVS ++DCKRC+ TT+
Sbjct: 61  EHVAYEYAKRGAYLALVARREHRLREVAEVAEILGSPFALLIHADVSNVDDCKRCVQTTL 120

Query: 121 AHFGRRHKLLGN------SLLHLQCDSTSKTKQRE-DNWNCF------------LRGMAT 180
            HFGR   L+ N      +L     D  +     + D W                RG   
Sbjct: 121 THFGRLDHLVNNAGITSINLFEEYDDPRNVASVMDTDFWGTVYCTYYAIPYLKQTRGKII 180

Query: 181 G---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFI 240
           G   +AA L    ++F   ASKAAV SFYETLR EI +++GITIVTPGLTESE+TQGKF+
Sbjct: 181 GIASSAAWLPTPRLSF-YSASKAAVISFYETLRVEIGRDIGITIVTPGLTESEMTQGKFM 240

Query: 241 SKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVP 300
            + G+MYLDQELRDA++SVMPI  VG   +AIV  GCRGDE+VT P W+R TF+W+  +P
Sbjct: 241 FEDGKMYLDQELRDAVMSVMPIEAVGSAAKAIVNGGCRGDEYVTEPPWIRMTFYWKMFLP 300

Query: 301 EVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSV 319
           E+VEW+N LFIMTG+SPT +FG+R+L+ TGLK FIYPPSV
Sbjct: 301 EMVEWLNYLFIMTGSSPTESFGKRVLEFTGLKPFIYPPSV 339

[Close]

BLAST of Moc03g29390 vs. ExPASy TrEMBL
Match: A0A6J1GSM5 (11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LOC111457123 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 3.1e-94
Identity = 197/344 (57.27%), Postives = 237/344 (68.90%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +NN LN +L   SL AF   LP YL FKFLS++ +SIFSEDV GKVVLITGASSGIG
Sbjct: 1   MDFLNNVLNFVLPITSLIAFIFFLPTYLFFKFLSFVFRSIFSEDVAGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           +H+AYEYAKRGAYL LVARRENRLREVA VA   GSP+ALVI AD+S+IEDCKRCI TT+
Sbjct: 61  KHMAYEYAKRGAYLVLVARRENRLREVAGVARFFGSPFALVIPADISRIEDCKRCIQTTV 120

Query: 121 AHFGRRHKLLGNSLLHLQCDSTSKTKQREDN---------WNCFL------------RGM 180
           A FGR   L+ N+   +   +  +     DN         W                RG 
Sbjct: 121 AVFGRLDHLVNNA--GVSSINLFEEYNNHDNAAPIMDVNFWGMVYCTYFAIPHLKQSRGK 180

Query: 181 ATG---AAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGK 240
             G   +AA L    ++F   ASKAAV SFYETLR E+ +++GITIVTPGL ESE+TQGK
Sbjct: 181 IIGISSSAAWLPVPRLSF-YSASKAAVISFYETLRVEVGRDIGITIVTPGLIESEMTQGK 240

Query: 241 FISKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRAL 300
           F+SK G +YLDQELRDA++S MPI P+   V  IVRS CRGD + T P WMR  F+++ L
Sbjct: 241 FMSKDGGLYLDQELRDAMVSAMPIMPIDSAVNGIVRSACRGDRYATEPRWMRMAFYYKTL 300

Query: 301 VPEVVEWINRLFIMTGTSPTHTFGQRLLQLTGLKTFIYPPSVLS 321
            PE+VEW+N L  MTG+SPT +FG+RLL+LTGLKT+ YP SV S
Sbjct: 301 WPELVEWVNYLIGMTGSSPTDSFGKRLLELTGLKTWFYPESVRS 341

[Close]

BLAST of Moc03g29390 vs. TAIR 10
Match: AT5G50600.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 256.5 bits (654), Expect = 2.9e-68
Identity = 147/346 (42.49%), Postives = 208/346 (60.12%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           M+L+N+FLN+     +       LP +  FKFL  I  +IFSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVSK +DC+R +D TI
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQREDN--WNC---------FLRG-----M 180
            HFGR   L+ N+      +    +  ++TK   D   W           +LR      +
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
           A  ++A  L +       ASKAA+ SF+ET+R E+  +V ITIVTPG  ESE+TQGK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 300
             G++ ++Q++RD  +   P+A   G  ++IV   CR   +VT P+W + T+ W+ L PE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 VVEWINRLFIMTGT--SPTHTFGQRLLQLTGLKTFIYPPSVLSPAI 324
           ++EW  RL  MTGT  S      +R++ + G+++ +YP S+ +P I
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPGVRSTLYPESIRTPEI 346

[Close]

BLAST of Moc03g29390 vs. TAIR 10
Match: AT5G50700.1 (hydroxysteroid dehydrogenase 1 )

HSP 1 Score: 256.5 bits (654), Expect = 2.9e-68
Identity = 147/346 (42.49%), Postives = 208/346 (60.12%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           M+L+N+FLN+     +       LP +  FKFL  I  +IFSE++ GKVVLITGASSGIG
Sbjct: 1   MELINDFLNLTAPFFTFFGLCFFLPPFYFFKFLQSIFSTIFSENLYGKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEYA RGA LAL ARR+NRL EVA +A  LGSP  + + ADVSK +DC+R +D TI
Sbjct: 61  EQLAYEYACRGACLALTARRKNRLEEVAEIARELGSPNVVTVHADVSKPDDCRRIVDDTI 120

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQREDN--WNC---------FLRG-----M 180
            HFGR   L+ N+      +    +  ++TK   D   W           +LR      +
Sbjct: 121 THFGRLDHLVNNAGMTQISMFENIEDITRTKAVLDTNFWGSVYTTRAALPYLRQSNGKIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
           A  ++A  L +       ASKAA+ SF+ET+R E+  +V ITIVTPG  ESE+TQGK+ S
Sbjct: 181 AMSSSAAWLTAPRMSFYNASKAALLSFFETMRIELGGDVHITIVTPGYIESELTQGKYFS 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 300
             G++ ++Q++RD  +   P+A   G  ++IV   CR   +VT P+W + T+ W+ L PE
Sbjct: 241 GEGELIVNQDMRDVQVGPFPVASASGCAKSIVNGVCRKQRYVTEPSWFKVTYLWKVLCPE 300

Query: 301 VVEWINRLFIMTGT--SPTHTFGQRLLQLTGLKTFIYPPSVLSPAI 324
           ++EW  RL  MTGT  S      +R++ + G+++ +YP S+ +P I
Sbjct: 301 LIEWGCRLLYMTGTGMSEDTALNKRIMDIPGVRSTLYPESIRTPEI 346

[Close]

BLAST of Moc03g29390 vs. TAIR 10
Match: AT5G50770.1 (hydroxysteroid dehydrogenase 6 )

HSP 1 Score: 207.2 bits (526), Expect = 2.0e-53
Identity = 125/311 (40.19%), Postives = 174/311 (55.95%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD +N  +N L   ++L A  V  P Y   K    I +++FSE+V GKVV+ITGA+SGIG
Sbjct: 1   MDSINKIINFLFPLLTLYALLVFYPTYQRLKSAVSICRNLFSENVAGKVVVITGAASGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           E LAYEY KRGAYLALV  R   L  VA +AEL GSP  L + ADVSK++DC+R I  T+
Sbjct: 61  EALAYEYGKRGAYLALVDIRGEPLFHVAALAELYGSPEVLPLVADVSKLQDCERFIRATV 120

Query: 121 AHFGRRHKLLGNS-----LLHLQCDSTSKTKQRED--NWNCFL--------------RGM 180
            HFGR   L+ N+           +  SK     D   W                  R +
Sbjct: 121 LHFGRLDHLVTNAGVAPLYFFADIEDVSKASPAMDINFWGSVYCTFFASPYLKKFRGRIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
              +    + S       ASKAAV +FYETLRTE   ++G+TIV PG+ +SE+++GKF++
Sbjct: 181 VIASGCGYIASPRLSFYCASKAAVIAFYETLRTEFGSDIGVTIVAPGIVDSEMSRGKFMT 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 291
           K G++ +D+ELRD  +SV+P+       +AI+RS CRGD ++  P W+      +    E
Sbjct: 241 KDGKLVVDKELRDVQMSVLPVESAERCAKAIMRSVCRGDRYLLEPDWIGCVILLKVFCSE 300

[Close]

BLAST of Moc03g29390 vs. TAIR 10
Match: AT3G47360.1 (hydroxysteroid dehydrogenase 3 )

HSP 1 Score: 171.4 bits (433), Expect = 1.2e-42
Identity = 111/311 (35.69%), Postives = 170/311 (54.66%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MD++   LN+LL  +++   F+  P YL+ K +  + K++  E+V  KVVLITGASSGIG
Sbjct: 1   MDILTTILNLLLPPLTIIFLFLFYPFYLLIKLVLCLRKNLHFENVARKVVLITGASSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EH+AYEYAK+GAYLALVARR +RL  VA  +  LGS   ++I  DVS +EDCK+ ID TI
Sbjct: 61  EHVAYEYAKKGAYLALVARRRDRLEIVAETSRQLGSGNVIIIPGDVSNVEDCKKFIDETI 120

Query: 121 AHFGRRHKLLGNSLLHLQCDSTSKTKQREDN-------WNCFL--------------RGM 180
            HFG+   L+ N+ +         T+ ++ N       W                  + +
Sbjct: 121 RHFGKLDHLINNAGVFQTVLFEDFTQIQDANPIMDINFWGTTYITYFAIPHLRKSKGKIV 180

Query: 181 ATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTESEVTQGKFIS 240
           A  + +  +   +     ASKAA+  F+ETLR E++ ++ ITIV PG+  +++T    I 
Sbjct: 181 AITSGSANIPLPLASIYAASKAALLRFFETLRIELSPDIKITIVLPGVVSTDMTTPHCIE 240

Query: 241 KTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTTFFWRALVPE 290
           K G  ++  E             V    +AI R   RG+ ++  P+WM+  F  + + PE
Sbjct: 241 KYGSDFILSE------------SVSKCAKAIFRGIGRGETYIEEPSWMKWLFIMKNVCPE 299

[Close]

BLAST of Moc03g29390 vs. TAIR 10
Match: AT5G50590.1 (hydroxysteroid dehydrogenase 4 )

HSP 1 Score: 169.5 bits (428), Expect = 4.6e-42
Identity = 116/311 (37.30%), Postives = 161/311 (51.77%), Query Frame = 0


Query: 1   MDLMNNFLNILLSRISLTAFFVILPAYLIFKFLSYIIKSIFSEDVDGKVVLITGASSGIG 60
           MDL N   NILL  ++++   V +P  + FK L +I     SE V+GKVV+ITG+SSGIG
Sbjct: 1   MDLNNKIFNILLPIVTVSFLLVFMPFSIFFKLLQFIRGCKESEKVNGKVVIITGSSSGIG 60

Query: 61  EHLAYEYAKRGAYLALVARRENRLREVAHVAELLGSPYALVIKADVSKIEDCKRCIDTTI 120
           EHLAYEYA+RGAYL LVARRE+RL+ VA     LGSP   V++ DVS I+DCKR +  TI
Sbjct: 61  EHLAYEYARRGAYLTLVARREDRLQVVADRCRKLGSPDVAVVRGDVSVIKDCKRFVQETI 120

Query: 121 AHFGRRHKLLGNS------------------------------LLHLQCDSTSKTKQRED 180
           + FGR   L+ N+                                H       KTK +  
Sbjct: 121 SRFGRLDHLVNNAGIAEAKFFEDYSEISDVLPIVNTNFWGPVYATHFAIPHLKKTKGK-- 180

Query: 181 NWNCFLRGMATGAAAELLHSVITFKLKASKAAVASFYETLRTEIAKEVGITIVTPGLTES 240
                +  +A+ A    +  +  +   ASKAA+ +FYETLR E+  EVG+TIV PGL E+
Sbjct: 181 -----IIAVASPAGWSGVPRMSIY--AASKAAMINFYETLRIELHPEVGVTIVFPGLIEN 240

Query: 241 EVTQGKFISKTGQMYLDQELRDALISVMPIAPVGGVVEAIVRSGCRGDEHVTAPAWMRTT 282
             T    +++          +     V+ I       +A+V   CRG   V  P+W+R  
Sbjct: 241 GNTNPDLLAE----------KQDWSQVVTIESAAECAKAVVNGICRGKTFVAEPSWVRVL 292

The following BLAST results are available for this feature:

BLAST of Moc03g29390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023000976.1	6.3e-102	59.08	11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita maxima]	[more]
KAG6584164.1	5.4e-101	58.84	11-beta-hydroxysteroid dehydrogenase 1B, partial [Cucurbita argyrosperma subsp. ...	[more]
XP_008446273.1	9.1e-101	60.88	PREDICTED: 11-beta-hydroxysteroid dehydrogenase 1B-like [Cucumis melo]	[more]
XP_022923773.1	9.1e-101	58.21	11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita moschata]	[more]
XP_023519994.1	9.1e-101	58.50	11-beta-hydroxysteroid dehydrogenase 1B-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc03g29390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0DKC5	4.0e-67	42.49	11-beta-hydroxysteroid dehydrogenase 1A OS=Arabidopsis thaliana OX=3702 GN=HSD1 ...	[more]
P0DKC6	4.0e-67	42.49	11-beta-hydroxysteroid dehydrogenase 1B OS=Arabidopsis thaliana OX=3702 GN=HSD1 ...	[more]
Q93W57	4.0e-67	43.02	11-beta-hydroxysteroid dehydrogenase A OS=Sesamum indicum OX=4182 GN=SOP2 PE=1 S...	[more]
A7LB60	9.0e-67	43.34	11-beta-hydroxysteroid dehydrogenase A OS=Arachis hypogaea OX=3818 GN=STO-A PE=1...	[more]
Q9LUE4	2.8e-52	40.19	11-beta-hydroxysteroid dehydrogenase-like 6 OS=Arabidopsis thaliana OX=3702 GN=H...	[more]

BLAST of Moc03g29390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KHB1	3.1e-102	59.08	11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita maxima OX=3661 GN=LOC1...	[more]
A0A6J1EAJ7	4.4e-101	58.21	11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]
A0A1S3BFC1	4.4e-101	60.88	11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucumis melo OX=3656 GN=LOC10348...	[more]
A0A0A0LTX2	1.1e-99	59.12	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G051670 PE=3 SV=1	[more]
A0A6J1GSM5	3.1e-94	57.27	11-beta-hydroxysteroid dehydrogenase 1B-like OS=Cucurbita moschata OX=3662 GN=LO...	[more]

BLAST of Moc03g29390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G50600.1	2.9e-68	42.49	hydroxysteroid dehydrogenase 1	[more]
AT5G50700.1	2.9e-68	42.49	hydroxysteroid dehydrogenase 1	[more]
AT5G50770.1	2.0e-53	40.19	hydroxysteroid dehydrogenase 6	[more]
AT3G47360.1	1.2e-42	35.69	hydroxysteroid dehydrogenase 3	[more]
AT5G50590.1	4.6e-42	37.30	hydroxysteroid dehydrogenase 4	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 176..195 score: 29.64 coord: 196..213 score: 20.28 coord: 49..66 score: 45.35
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 48..135 e-value: 1.9E-14 score: 53.6
None	No IPR available	GENE3D	3.40.50.720		coord: 35..291 e-value: 1.8E-31 score: 111.2
None	No IPR available	PANTHER	PTHR43391	RETINOL DEHYDROGENASE-RELATED	coord: 176..318 coord: 1..133
None	No IPR available	PANTHER	PTHR43391:SF68	11-BETA-HYDROXYSTEROID DEHYDROGENASE 1B-LIKE ISOFORM X1	coord: 176..318 coord: 1..133
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 43..290

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g29390.1	Moc03g29390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016491	oxidoreductase activity