Moc03g28950 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g28950
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionRetrovirus-related Pol polyprotein from transposon RE1
Locationchr3: 20737608 .. 20738970 (-)
RNA-Seq ExpressionMoc03g28950
SyntenyMoc03g28950
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACTAAGAGAGGCCTAATCAGTCCCCCGAGAGCCTTTTCTGGACCAAACAATGCCGCCGCAAATCCGCCGATGAGGCCCAATTACAGGATGTCACCACCCCCCCGACCCACGACGGTGGTCAATCCCAGCGAGCAGATGTTTTACGAGCATTGGTTAAAAGCCACTTCTAGTTCTAATGCCATACGTGCAACCCATATCGCTCCGCCAAATCCTTACGATCTGCAGAATCATCAGAAGAAACTAGACCACAACAACAACAACAATTCCAGCACCAAAACCACAGCGCCCCCTCCTACTCGCCACCAGAGGCTGCGTAATGGGGCCACGCTAATTTCCAACAACACTGCTCCAAGACCAAAAGGACCCACTGCTTCTGGTTCCAGATCCGATCCCCTTGACGCCAATCTGATCGCTCCAAAATTTTCTGATTCTCCAAATAATTCTTCTCCCAACCATTTATCCATCCATGGATCGGGTCAACGCCAAAATATCAGCAACGTCGGTAATAACGGTGGAACCGCTTATACTTCACTTGCTCATCCTCAACTTAACAATCACTTACACAAACTTTCTAGAGGCGGAAGCGGTGGCAATGACGGTGAGACTTCTTACCTTGTATATTCTTAAAGATACAACCATCGAAAACAAAACAACTTTAAAAAGATTTATAATAAACGTAGACTACCATGGTAATTGCAAATTGGTTTTAAAATCCATCAATATTCATATAGGTCAATTATTCCATAATTGGTAGTTTAATTGTTTTTATTTAGTTAAGCAGTTAATTTAGTTTATTTCTTTTATTAATTTGTCAATTTTTTTTGAGATATATTAATTTGTCAATTAGTTTGGAGTTTAATTGCAATTTCTTATATAATGTTTAAAGTTTTAAAAGTCAAATAATGCAAAAACATTTTGAATTTAAAATGTAAGGCTAACTAGAACTATGCTCATTTTTTTAGCTTATCCCGAAATCCCCATATACGTAGGTAAAATTCCTATATATGGTGGTCAAGATGATCACATTGTCCTTCATTCGAAATGTTCTTCACAAAGCAATGTTCCGAGAATATTCGAAATTTACAAGGACATTGCATCTCATCGTCAAGGAAACTCATCTATTACATCTTACTTCACAAGGTTAAAGACATTGTGGGACGAACTTGAAACCTACAATGATCTTTCTCAATGTTGTTCCTCCGGTGAACATGTGGAGAGAGAAAAAGTTATGCAATTTCTCGTGGGGTTAAATGATCCATATTCCACTATCTGCCACCAAATCCTTCTTATTCGGCCGTTTCCAACAGTGGAGAAAGCATATTCTATTGTAATTCGAGAAGAGAAACGGATGTGGAGCTGA

mRNA sequence

ATGACTAAGAGAGGCCTAATCAGTCCCCCGAGAGCCTTTTCTGGACCAAACAATGCCGCCGCAAATCCGCCGATGAGGCCCAATTACAGGATGTCACCACCCCCCCGACCCACGACGGTGGTCAATCCCAGCGAGCAGATGTTTTACGAGCATTGGTTAAAAGCCACTTCTAGTTCTAATGCCATACGTGCAACCCATATCGCTCCGCCAAATCCTTACGATCTGCAGAATCATCAGAAGAAACTAGACCACAACAACAACAACAATTCCAGCACCAAAACCACAGCGCCCCCTCCTACTCGCCACCAGAGGCTGCGTAATGGGGCCACGCTAATTTCCAACAACACTGCTCCAAGACCAAAAGGACCCACTGCTTCTGGTTCCAGATCCGATCCCCTTGACGCCAATCTGATCGCTCCAAAATTTTCTGATTCTCCAAATAATTCTTCTCCCAACCATTTATCCATCCATGGATCGGGTCAACGCCAAAATATCAGCAACGTCGGTAATAACGGTGGAACCGCTTATACTTCACTTGCTCATCCTCAACTTAACAATCACTTACACAAACTTTCTAGAGGCGGAAGCGGTGGCAATGACGGTAAAATTCCTATATATGGTGGTCAAGATGATCACATTGTCCTTCATTCGAAATGTTCTTCACAAAGCAATGTTCCGAGAATATTCGAAATTTACAAGGACATTGCATCTCATCGTCAAGGAAACTCATCTATTACATCTTACTTCACAAGGTTAAAGACATTGTGGGACGAACTTGAAACCTACAATGATCTTTCTCAATGTTGTTCCTCCGGTGAACATGTGGAGAGAGAAAAAGTTATGCAATTTCTCGTGGGGTTAAATGATCCATATTCCACTATCTGCCACCAAATCCTTCTTATTCGGCCGTTTCCAACAGTGGAGAAAGCATATTCTATTGTAATTCGAGAAGAGAAACGGATGTGGAGCTGA

Coding sequence (CDS)

ATGACTAAGAGAGGCCTAATCAGTCCCCCGAGAGCCTTTTCTGGACCAAACAATGCCGCCGCAAATCCGCCGATGAGGCCCAATTACAGGATGTCACCACCCCCCCGACCCACGACGGTGGTCAATCCCAGCGAGCAGATGTTTTACGAGCATTGGTTAAAAGCCACTTCTAGTTCTAATGCCATACGTGCAACCCATATCGCTCCGCCAAATCCTTACGATCTGCAGAATCATCAGAAGAAACTAGACCACAACAACAACAACAATTCCAGCACCAAAACCACAGCGCCCCCTCCTACTCGCCACCAGAGGCTGCGTAATGGGGCCACGCTAATTTCCAACAACACTGCTCCAAGACCAAAAGGACCCACTGCTTCTGGTTCCAGATCCGATCCCCTTGACGCCAATCTGATCGCTCCAAAATTTTCTGATTCTCCAAATAATTCTTCTCCCAACCATTTATCCATCCATGGATCGGGTCAACGCCAAAATATCAGCAACGTCGGTAATAACGGTGGAACCGCTTATACTTCACTTGCTCATCCTCAACTTAACAATCACTTACACAAACTTTCTAGAGGCGGAAGCGGTGGCAATGACGGTAAAATTCCTATATATGGTGGTCAAGATGATCACATTGTCCTTCATTCGAAATGTTCTTCACAAAGCAATGTTCCGAGAATATTCGAAATTTACAAGGACATTGCATCTCATCGTCAAGGAAACTCATCTATTACATCTTACTTCACAAGGTTAAAGACATTGTGGGACGAACTTGAAACCTACAATGATCTTTCTCAATGTTGTTCCTCCGGTGAACATGTGGAGAGAGAAAAAGTTATGCAATTTCTCGTGGGGTTAAATGATCCATATTCCACTATCTGCCACCAAATCCTTCTTATTCGGCCGTTTCCAACAGTGGAGAAAGCATATTCTATTGTAATTCGAGAAGAGAAACGGATGTGGAGCTGA

Protein sequence

MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSNAIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRPKGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEKAYSIVIREEKRMWS
Homology
BLAST of Moc03g28950 vs. NCBI nr
Match: XP_022137506.1 (uncharacterized protein LOC111008934 isoform X2 [Momordica charantia])

HSP 1 Score: 657.9 bits (1696), Expect = 4.5e-185
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60
           MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN
Sbjct: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60

Query: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120
           AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP
Sbjct: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120

Query: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180
           KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA
Sbjct: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180

Query: 181 HPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQ 240
           HPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQ
Sbjct: 181 HPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQ 240

Query: 241 GNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILL 300
           GNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILL
Sbjct: 241 GNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILL 300

Query: 301 IRPFPTVEKAYSIVIREEKRMWS 324
           IRPFPTVEKAYSIVIREEKRMWS
Sbjct: 301 IRPFPTVEKAYSIVIREEKRMWS 323

BLAST of Moc03g28950 vs. NCBI nr
Match: XP_022137505.1 (uncharacterized protein LOC111008934 isoform X1 [Momordica charantia])

HSP 1 Score: 650.2 bits (1676), Expect = 9.3e-183
Identity = 323/332 (97.29%), Postives = 323/332 (97.29%), Query Frame = 0

Query: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60
           MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN
Sbjct: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60

Query: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120
           AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP
Sbjct: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120

Query: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180
           KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA
Sbjct: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180

Query: 181 HPQLNNHLHKLSRGGSGGND---------GKIPIYGGQDDHIVLHSKCSSQSNVPRIFEI 240
           HPQLNNHLHKLSRGGSGGND         GKIPIYGGQDDHIVLHSKCSSQSNVPRIFEI
Sbjct: 181 HPQLNNHLHKLSRGGSGGNDAYPEIPIYVGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEI 240

Query: 241 YKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPY 300
           YKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPY
Sbjct: 241 YKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPY 300

Query: 301 STICHQILLIRPFPTVEKAYSIVIREEKRMWS 324
           STICHQILLIRPFPTVEKAYSIVIREEKRMWS
Sbjct: 301 STICHQILLIRPFPTVEKAYSIVIREEKRMWS 332

BLAST of Moc03g28950 vs. NCBI nr
Match: XP_022137556.1 (uncharacterized protein LOC111008978 [Momordica charantia])

HSP 1 Score: 272.7 bits (696), Expect = 4.1e-69
Identity = 185/363 (50.96%), Postives = 223/363 (61.43%), Query Frame = 0

Query: 4   RGLISPPRAFSGPNNAAANPPMRP-----NYRMSPPPRPT--TVVNPSEQMFYEHWLKAT 63
           RGLISPPR+F+G        P RP     ++ MS   RPT  +V +PS+        +  
Sbjct: 3   RGLISPPRSFAG-------SPSRPLADDLHFYMSQSRRPTASSVADPSDH-------RTP 62

Query: 64  SSSNAIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRL--RN------- 123
             +   +  H A  N  DLQN+ KKLD  NNNNSSTKTTA   +    +  RN       
Sbjct: 63  PRTRTSQRRHTAEKNADDLQNYLKKLD-RNNNNSSTKTTAKISSSRPSISPRNGLEIEQP 122

Query: 124 -GATLISNNTAPRPKGPTASGS----RSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQR 183
            G T IS+N AP+PK   A  S     S   +ANLIAPKF DSP+NSSP HLSIHGSGQR
Sbjct: 123 RGPTPISHNAAPKPKPAPAIDSPAPDASTANNANLIAPKFFDSPHNSSPTHLSIHGSGQR 182

Query: 184 QNISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGND-----GKIPIYGGQD-DHIVLH 243
           QNI+NVG+NGG ++T LAHPQLN++LHKLS  G    +     G+IPIY G+   +IVL 
Sbjct: 183 QNINNVGSNGGASHTPLAHPQLNSNLHKLSGDGDACVEIPIYIGEIPIYVGKHVANIVLR 242

Query: 244 -------------SKCSSQSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLWDELETYN 303
                         + SSQSN   IF+IYK  ASHRQ +SS+TSYF +LK LWD+L  Y+
Sbjct: 243 ENSIYKPVGSNTKQEWSSQSNDSEIFQIYKGFASHRQKSSSVTSYFKKLKILWDQLRNYS 302

Query: 304 DLSQCCSSG------EHVEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEKAYSIVIRE 321
           DL QC S G      EHVEREKV+QFLVGLND YSTI  QIL IRP PTVEKAY + I+E
Sbjct: 303 DLPQCYSLGAKQKLSEHVEREKVIQFLVGLNDSYSTIVSQILQIRPLPTVEKAYFLTIQE 350

BLAST of Moc03g28950 vs. NCBI nr
Match: XP_022137024.1 (uncharacterized protein LOC111008588 [Momordica charantia] >XP_022137025.1 uncharacterized protein LOC111008588 [Momordica charantia] >XP_022137026.1 uncharacterized protein LOC111008588 [Momordica charantia])

HSP 1 Score: 252.3 bits (643), Expect = 5.7e-63
Identity = 183/372 (49.19%), Postives = 208/372 (55.91%), Query Frame = 0

Query: 1   MTKRGLISPPRAFSGP------NNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLK 60
           MT RGLISPPR+ S P      NN A NPP RPNY MSP  RPTT    +EQ   +   K
Sbjct: 47  MTLRGLISPPRSRSSPRDFRPHNNGAPNPPSRPNY-MSPRRRPTT----AEQQHTQTHRK 106

Query: 61  ATSSSNAIRATHIAP------------------PNPYDLQNHQKKLDHNNNNNSSTKTTA 120
              S+ A RAT  +P                  PNP DL N QKKLD            +
Sbjct: 107 --PSATATRATKKSPPRIRPSPKPTAPIVPSRRPNPNDLHN-QKKLDTKTTPKIGGAKPS 166

Query: 121 PPPTRHQRLRNGA------TLISNNTAPRPKG--PTASGSRSDPLDANLIAPKFSDSPNN 180
            P T+ QRLRNG       T IS NT P+PK     AS SRSD       +P  + SP  
Sbjct: 167 SPRTQPQRLRNGVEPPRGPTPISKNTTPKPKPAIAIASASRSD-------SPAANSSP-- 226

Query: 181 SSPNHLSIHGSGQRQNISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGNDGKIPIYGG 240
            S  HL   GS Q  ++ N     G  YT LA PQLNNHL +LS            I G 
Sbjct: 227 -SSKHLPSPGSAQHHDMKNHSPYSGGTYTPLADPQLNNHLQRLS------------IDGK 286

Query: 241 QDDHIVLHSKC------------SSQSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLW 300
               I+LH+              S QSN PRIF+IYKDIASHRQ NSS+TSYFT+LK LW
Sbjct: 287 DLASIILHANSIYESIGSDTMEESFQSNAPRIFQIYKDIASHRQENSSVTSYFTKLKILW 346

Query: 301 DELETY-NDLSQCCSSGE------HVEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEK 322
           DELETY +D+ QCCS G       HVEREKVMQFL+GLN+ YSTIC QILLI+PFPT+EK
Sbjct: 347 DELETYSDDVPQCCSCGAMEKLSGHVEREKVMQFLMGLNNSYSTICPQILLIQPFPTMEK 388

BLAST of Moc03g28950 vs. NCBI nr
Match: XP_022137569.1 (uncharacterized protein LOC111008986 [Momordica charantia])

HSP 1 Score: 209.9 bits (533), Expect = 3.2e-50
Identity = 157/344 (45.64%), Postives = 187/344 (54.36%), Query Frame = 0

Query: 1   MTKRGLISPPRA-FS-----GPNNAAANPPMRPNYRMSPPPRPTT--VVNPSEQMFYEHW 60
           M  RGLISPP++ FS       NNAAANPP  PNY MS   R T   VVNP +Q      
Sbjct: 4   MRGRGLISPPKSRFSVTESDSQNNAAANPPSMPNY-MSATRRSTAARVVNPKQQQTTH-- 63

Query: 61  LKATSSSNAIRATHIAPPNPYDLQNHQKK------LDHNNNNNSSTKTTAPPPTRHQRLR 120
            K T  SNAIRAT  + P P  +   +++        + +N+NS+TK T    T     R
Sbjct: 64  TKPTFGSNAIRATKNSSPKPTPVVPSRRRPTWAPNNPNGHNDNSTTKLTIAKITTS---R 123

Query: 121 NGATLISNNTAPRPKGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQ--N 180
           N      N    +P+GPT     S                        S HGSG  Q  N
Sbjct: 124 NSNV---NGVQQQPRGPTLISVASG-----------------------SSHGSGHHQDAN 183

Query: 181 ISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIV---LHSKCSS 240
            +N+      + T + HP + N L +LS  G   +  K+       D  V      +CS 
Sbjct: 184 NNNIEGEEEDSTTVIGHPHVINQLQQLSIDGK--HHAKMVFRANSMDESVGPYTKEECSP 243

Query: 241 QSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCS-------SGEH 300
           QSN  RI EIYKDIASHRQGNSSITSYFT+L+TLW+ELETY+DL QCCS         + 
Sbjct: 244 QSNAERILEIYKDIASHRQGNSSITSYFTKLETLWEELETYSDLPQCCSYSATDQKPSKL 303

Query: 301 VEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEKAYSIVIREE 319
           VEREKVMQFLVGLND YSTIC QILLIRPFPTVEKAYSI+I +E
Sbjct: 304 VEREKVMQFLVGLNDSYSTICSQILLIRPFPTVEKAYSIIIMQE 313

BLAST of Moc03g28950 vs. ExPASy TrEMBL
Match: A0A6J1C6T8 (uncharacterized protein LOC111008934 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008934 PE=4 SV=1)

HSP 1 Score: 657.9 bits (1696), Expect = 2.2e-185
Identity = 323/323 (100.00%), Postives = 323/323 (100.00%), Query Frame = 0

Query: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60
           MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN
Sbjct: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60

Query: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120
           AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP
Sbjct: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120

Query: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180
           KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA
Sbjct: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180

Query: 181 HPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQ 240
           HPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQ
Sbjct: 181 HPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEIYKDIASHRQ 240

Query: 241 GNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILL 300
           GNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILL
Sbjct: 241 GNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPYSTICHQILL 300

Query: 301 IRPFPTVEKAYSIVIREEKRMWS 324
           IRPFPTVEKAYSIVIREEKRMWS
Sbjct: 301 IRPFPTVEKAYSIVIREEKRMWS 323

BLAST of Moc03g28950 vs. ExPASy TrEMBL
Match: A0A6J1C6U3 (uncharacterized protein LOC111008934 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008934 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.5e-183
Identity = 323/332 (97.29%), Postives = 323/332 (97.29%), Query Frame = 0

Query: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60
           MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN
Sbjct: 1   MTKRGLISPPRAFSGPNNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLKATSSSN 60

Query: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120
           AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP
Sbjct: 61  AIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRLRNGATLISNNTAPRP 120

Query: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180
           KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA
Sbjct: 121 KGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQNISNVGNNGGTAYTSLA 180

Query: 181 HPQLNNHLHKLSRGGSGGND---------GKIPIYGGQDDHIVLHSKCSSQSNVPRIFEI 240
           HPQLNNHLHKLSRGGSGGND         GKIPIYGGQDDHIVLHSKCSSQSNVPRIFEI
Sbjct: 181 HPQLNNHLHKLSRGGSGGNDAYPEIPIYVGKIPIYGGQDDHIVLHSKCSSQSNVPRIFEI 240

Query: 241 YKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPY 300
           YKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPY
Sbjct: 241 YKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSGEHVEREKVMQFLVGLNDPY 300

Query: 301 STICHQILLIRPFPTVEKAYSIVIREEKRMWS 324
           STICHQILLIRPFPTVEKAYSIVIREEKRMWS
Sbjct: 301 STICHQILLIRPFPTVEKAYSIVIREEKRMWS 332

BLAST of Moc03g28950 vs. ExPASy TrEMBL
Match: A0A6J1C6Z1 (uncharacterized protein LOC111008978 OS=Momordica charantia OX=3673 GN=LOC111008978 PE=4 SV=1)

HSP 1 Score: 272.7 bits (696), Expect = 2.0e-69
Identity = 185/363 (50.96%), Postives = 223/363 (61.43%), Query Frame = 0

Query: 4   RGLISPPRAFSGPNNAAANPPMRP-----NYRMSPPPRPT--TVVNPSEQMFYEHWLKAT 63
           RGLISPPR+F+G        P RP     ++ MS   RPT  +V +PS+        +  
Sbjct: 3   RGLISPPRSFAG-------SPSRPLADDLHFYMSQSRRPTASSVADPSDH-------RTP 62

Query: 64  SSSNAIRATHIAPPNPYDLQNHQKKLDHNNNNNSSTKTTAPPPTRHQRL--RN------- 123
             +   +  H A  N  DLQN+ KKLD  NNNNSSTKTTA   +    +  RN       
Sbjct: 63  PRTRTSQRRHTAEKNADDLQNYLKKLD-RNNNNSSTKTTAKISSSRPSISPRNGLEIEQP 122

Query: 124 -GATLISNNTAPRPKGPTASGS----RSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQR 183
            G T IS+N AP+PK   A  S     S   +ANLIAPKF DSP+NSSP HLSIHGSGQR
Sbjct: 123 RGPTPISHNAAPKPKPAPAIDSPAPDASTANNANLIAPKFFDSPHNSSPTHLSIHGSGQR 182

Query: 184 QNISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGND-----GKIPIYGGQD-DHIVLH 243
           QNI+NVG+NGG ++T LAHPQLN++LHKLS  G    +     G+IPIY G+   +IVL 
Sbjct: 183 QNINNVGSNGGASHTPLAHPQLNSNLHKLSGDGDACVEIPIYIGEIPIYVGKHVANIVLR 242

Query: 244 -------------SKCSSQSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLWDELETYN 303
                         + SSQSN   IF+IYK  ASHRQ +SS+TSYF +LK LWD+L  Y+
Sbjct: 243 ENSIYKPVGSNTKQEWSSQSNDSEIFQIYKGFASHRQKSSSVTSYFKKLKILWDQLRNYS 302

Query: 304 DLSQCCSSG------EHVEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEKAYSIVIRE 321
           DL QC S G      EHVEREKV+QFLVGLND YSTI  QIL IRP PTVEKAY + I+E
Sbjct: 303 DLPQCYSLGAKQKLSEHVEREKVIQFLVGLNDSYSTIVSQILQIRPLPTVEKAYFLTIQE 350

BLAST of Moc03g28950 vs. ExPASy TrEMBL
Match: A0A6J1C5Z8 (uncharacterized protein LOC111008588 OS=Momordica charantia OX=3673 GN=LOC111008588 PE=4 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 183/372 (49.19%), Postives = 208/372 (55.91%), Query Frame = 0

Query: 1   MTKRGLISPPRAFSGP------NNAAANPPMRPNYRMSPPPRPTTVVNPSEQMFYEHWLK 60
           MT RGLISPPR+ S P      NN A NPP RPNY MSP  RPTT    +EQ   +   K
Sbjct: 47  MTLRGLISPPRSRSSPRDFRPHNNGAPNPPSRPNY-MSPRRRPTT----AEQQHTQTHRK 106

Query: 61  ATSSSNAIRATHIAP------------------PNPYDLQNHQKKLDHNNNNNSSTKTTA 120
              S+ A RAT  +P                  PNP DL N QKKLD            +
Sbjct: 107 --PSATATRATKKSPPRIRPSPKPTAPIVPSRRPNPNDLHN-QKKLDTKTTPKIGGAKPS 166

Query: 121 PPPTRHQRLRNGA------TLISNNTAPRPKG--PTASGSRSDPLDANLIAPKFSDSPNN 180
            P T+ QRLRNG       T IS NT P+PK     AS SRSD       +P  + SP  
Sbjct: 167 SPRTQPQRLRNGVEPPRGPTPISKNTTPKPKPAIAIASASRSD-------SPAANSSP-- 226

Query: 181 SSPNHLSIHGSGQRQNISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGNDGKIPIYGG 240
            S  HL   GS Q  ++ N     G  YT LA PQLNNHL +LS            I G 
Sbjct: 227 -SSKHLPSPGSAQHHDMKNHSPYSGGTYTPLADPQLNNHLQRLS------------IDGK 286

Query: 241 QDDHIVLHSKC------------SSQSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLW 300
               I+LH+              S QSN PRIF+IYKDIASHRQ NSS+TSYFT+LK LW
Sbjct: 287 DLASIILHANSIYESIGSDTMEESFQSNAPRIFQIYKDIASHRQENSSVTSYFTKLKILW 346

Query: 301 DELETY-NDLSQCCSSGE------HVEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEK 322
           DELETY +D+ QCCS G       HVEREKVMQFL+GLN+ YSTIC QILLI+PFPT+EK
Sbjct: 347 DELETYSDDVPQCCSCGAMEKLSGHVEREKVMQFLMGLNNSYSTICPQILLIQPFPTMEK 388

BLAST of Moc03g28950 vs. ExPASy TrEMBL
Match: A0A6J1C7L7 (uncharacterized protein LOC111008986 OS=Momordica charantia OX=3673 GN=LOC111008986 PE=4 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 1.6e-50
Identity = 157/344 (45.64%), Postives = 187/344 (54.36%), Query Frame = 0

Query: 1   MTKRGLISPPRA-FS-----GPNNAAANPPMRPNYRMSPPPRPTT--VVNPSEQMFYEHW 60
           M  RGLISPP++ FS       NNAAANPP  PNY MS   R T   VVNP +Q      
Sbjct: 4   MRGRGLISPPKSRFSVTESDSQNNAAANPPSMPNY-MSATRRSTAARVVNPKQQQTTH-- 63

Query: 61  LKATSSSNAIRATHIAPPNPYDLQNHQKK------LDHNNNNNSSTKTTAPPPTRHQRLR 120
            K T  SNAIRAT  + P P  +   +++        + +N+NS+TK T    T     R
Sbjct: 64  TKPTFGSNAIRATKNSSPKPTPVVPSRRRPTWAPNNPNGHNDNSTTKLTIAKITTS---R 123

Query: 121 NGATLISNNTAPRPKGPTASGSRSDPLDANLIAPKFSDSPNNSSPNHLSIHGSGQRQ--N 180
           N      N    +P+GPT     S                        S HGSG  Q  N
Sbjct: 124 NSNV---NGVQQQPRGPTLISVASG-----------------------SSHGSGHHQDAN 183

Query: 181 ISNVGNNGGTAYTSLAHPQLNNHLHKLSRGGSGGNDGKIPIYGGQDDHIV---LHSKCSS 240
            +N+      + T + HP + N L +LS  G   +  K+       D  V      +CS 
Sbjct: 184 NNNIEGEEEDSTTVIGHPHVINQLQQLSIDGK--HHAKMVFRANSMDESVGPYTKEECSP 243

Query: 241 QSNVPRIFEIYKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCS-------SGEH 300
           QSN  RI EIYKDIASHRQGNSSITSYFT+L+TLW+ELETY+DL QCCS         + 
Sbjct: 244 QSNAERILEIYKDIASHRQGNSSITSYFTKLETLWEELETYSDLPQCCSYSATDQKPSKL 303

Query: 301 VEREKVMQFLVGLNDPYSTICHQILLIRPFPTVEKAYSIVIREE 319
           VEREKVMQFLVGLND YSTIC QILLIRPFPTVEKAYSI+I +E
Sbjct: 304 VEREKVMQFLVGLNDSYSTICSQILLIRPFPTVEKAYSIIIMQE 313

BLAST of Moc03g28950 vs. TAIR 10
Match: AT1G21280.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Has 707 Blast hits to 705 proteins in 25 species: Archae - 0; Bacteria - 0; Metazoa - 4; Fungi - 0; Plants - 703; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 55.1 bits (131), Expect = 1.2e-07
Identity = 30/107 (28.04%), Postives = 57/107 (53.27%), Query Frame = 0

Query: 227 RIFEIYKDIASHRQGNSSITSYFTRLKTLWDELETYNDLSQCCSSG----------EHVE 286
           +I+++ + +A+ RQG  S+  YF +L  +W EL  Y  + +C   G          E  E
Sbjct: 126 KIYQLRRRLATLRQGGDSVEEYFGKLSKVWMELSEYAPIPECKCGGCNCECTKRAEEARE 185

Query: 287 REKVMQFLVG--LNDPYSTICHQILLIRPFPTVEKAYSIVIREEKRM 322
           +E+  +FL+G  LN  +  +  +I+  +P P++ +A+++V   E  M
Sbjct: 186 KEQRYEFLMGLKLNQGFEAVTTKIMFQKPPPSLHEAFAMVKDAESMM 232

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022137506.14.5e-185100.00uncharacterized protein LOC111008934 isoform X2 [Momordica charantia][more]
XP_022137505.19.3e-18397.29uncharacterized protein LOC111008934 isoform X1 [Momordica charantia][more]
XP_022137556.14.1e-6950.96uncharacterized protein LOC111008978 [Momordica charantia][more]
XP_022137024.15.7e-6349.19uncharacterized protein LOC111008588 [Momordica charantia] >XP_022137025.1 uncha... [more]
XP_022137569.13.2e-5045.64uncharacterized protein LOC111008986 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1C6T82.2e-185100.00uncharacterized protein LOC111008934 isoform X2 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C6U34.5e-18397.29uncharacterized protein LOC111008934 isoform X1 OS=Momordica charantia OX=3673 G... [more]
A0A6J1C6Z12.0e-6950.96uncharacterized protein LOC111008978 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A6J1C5Z82.7e-6349.19uncharacterized protein LOC111008588 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
A0A6J1C7L71.6e-5045.64uncharacterized protein LOC111008986 OS=Momordica charantia OX=3673 GN=LOC111008... [more]
Match NameE-valueIdentityDescription
AT1G21280.11.2e-0728.04CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Ha... [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005162Retrotransposon gag domainPFAMPF03732Retrotrans_gagcoord: 233..289
e-value: 2.1E-5
score: 24.7
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 65..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..123
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 141..179
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..37
NoneNo IPR availablePANTHERPTHR34222FAMILY NOT NAMEDcoord: 220..320
NoneNo IPR availablePANTHERPTHR34222:SF6OS02G0671800 PROTEINcoord: 220..320

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g28950.1Moc03g28950.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006259 DNA metabolic process
biological_process GO:0007127 meiosis I
cellular_component GO:0005737 cytoplasm
cellular_component GO:0043231 intracellular membrane-bounded organelle