Moc03g28400 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g28400
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	DUF4228 domain-containing protein
Location	chr3: 20371127 .. 20371480 (-)
RNA-Seq Expression	Moc03g28400
Synteny	Moc03g28400

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTAACTGTTGCAGAAGCCAGTCCTCTACATTGGTGTGGGCCGGAGACGATTGGGGCTCCTTCAATTCCAAGCACGGCCGCGGCGGAAACGCAACCACGTCCGGCGGCGATACTGAGAAGCAGAGGCTACTTGGCCCAAAAAAAGCAGCGGCAACGGCGGCGGCGGGGGAAGTGAAAATCAAGATGACGAAGAAAGAGCTGGAGGATCTGGTGGAAAAGCTAGAGAAGCAAGGGCTGAGCCTGGAAGAAGTTATTGGGCGGATGGTGAAGGGCGGCGAAGAAGATGAATATGAAATGGAGCACCACCGCTCATGGAGGCCTTCTCTTCAGAGCATTCCAGAGGATTACTAA

mRNA sequence

ATGGGTAACTGTTGCAGAAGCCAGTCCTCTACATTGGTGTGGGCCGGAGACGATTGGGGCTCCTTCAATTCCAAGCACGGCCGCGGCGGAAACGCAACCACGTCCGGCGGCGATACTGAGAAGCAGAGGCTACTTGGCCCAAAAAAAGCAGCGGCAACGGCGGCGGCGGGGGAAGTGAAAATCAAGATGACGAAGAAAGAGCTGGAGGATCTGGTGGAAAAGCTAGAGAAGCAAGGGCTGAGCCTGGAAGAAGTTATTGGGCGGATGGTGAAGGGCGGCGAAGAAGATGAATATGAAATGGAGCACCACCGCTCATGGAGGCCTTCTCTTCAGAGCATTCCAGAGGATTACTAA

Coding sequence (CDS)

ATGGGTAACTGTTGCAGAAGCCAGTCCTCTACATTGGTGTGGGCCGGAGACGATTGGGGCTCCTTCAATTCCAAGCACGGCCGCGGCGGAAACGCAACCACGTCCGGCGGCGATACTGAGAAGCAGAGGCTACTTGGCCCAAAAAAAGCAGCGGCAACGGCGGCGGCGGGGGAAGTGAAAATCAAGATGACGAAGAAAGAGCTGGAGGATCTGGTGGAAAAGCTAGAGAAGCAAGGGCTGAGCCTGGAAGAAGTTATTGGGCGGATGGTGAAGGGCGGCGAAGAAGATGAATATGAAATGGAGCACCACCGCTCATGGAGGCCTTCTCTTCAGAGCATTCCAGAGGATTACTAA

Protein sequence

MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY

Homology

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BLAST of Moc03g28400 vs. NCBI nr
Match: XP_022140150.1 (uncharacterized protein LOC111010880 [Momordica charantia])

HSP 1 Score: 232.6 bits (592), Expect = 1.7e-57
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVK 60
           MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVK
Sbjct: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVK 60

Query: 61  IKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 118
           IKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY
Sbjct: 61  IKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 117

[Close]

BLAST of Moc03g28400 vs. NCBI nr
Match: KAA0056859.1 (DUF4228 domain-containing protein [Cucumis melo var. makuwa] >TYJ99362.1 DUF4228 domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 174.1 bits (440), Expect = 7.1e-40
Identity = 92/119 (77.31%), Postives = 105/119 (88.24%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGR--GGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+V+AGDDWGSF+SKHGR  GGNATTSGGD EK RLLG +K AA+ +   
Sbjct: 1   MGNCCRSQSSTMVFAGDDWGSFSSKHGRGCGGNATTSGGDPEKHRLLGDQKEAASCSQPL 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 118
           VKIKMTK+ELE LV+KLE QGL+LE+VIGRM+KG  E+EYE+EHHRSWRPSLQSIPEDY
Sbjct: 61  VKIKMTKRELEVLVKKLEMQGLTLEQVIGRMMKG--EEEYEIEHHRSWRPSLQSIPEDY 117

[Close]

BLAST of Moc03g28400 vs. NCBI nr
Match: XP_038895954.1 (uncharacterized protein LOC120084128 [Benincasa hispida])

HSP 1 Score: 171.4 bits (433), Expect = 4.6e-39
Identity = 91/119 (76.47%), Postives = 105/119 (88.24%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKH--GRGGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+V+ GDDWGSF+SKH  GRGGNATTSGGD EK RLLG +K AA+ +  +
Sbjct: 1   MGNCCRSQSSTMVFVGDDWGSFSSKHGRGRGGNATTSGGDPEKYRLLG-EKEAASCSQPQ 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 118
           VKIKM+K+ELEDLV+KLE QG+SLE+VIGRMV  G EDE+E+EHHRSWRPSLQSIPEDY
Sbjct: 61  VKIKMSKRELEDLVKKLEMQGMSLEQVIGRMVMNG-EDEFEVEHHRSWRPSLQSIPEDY 117

[Close]

BLAST of Moc03g28400 vs. NCBI nr
Match: XP_022933211.1 (uncharacterized protein LOC111440057 [Cucurbita moschata])

HSP 1 Score: 153.3 bits (386), Expect = 1.3e-33
Identity = 82/117 (70.09%), Postives = 99/117 (84.62%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKH--GRGGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+++AGDDWGSF+SKH  GRGGNA+TS GD EK RLLG +K  ++    +
Sbjct: 1   MGNCCRSQSSTMIFAGDDWGSFSSKHGRGRGGNASTSSGDPEKYRLLG-EKQTSSCPQPQ 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPE 116
           VKIKMTK+ELEDLV+ LE +GLSLE+VIGRM+   +EDE++ EHHRSWRPSLQSIPE
Sbjct: 61  VKIKMTKRELEDLVKNLEIEGLSLEQVIGRMM--NDEDEFQGEHHRSWRPSLQSIPE 114

[Close]

BLAST of Moc03g28400 vs. NCBI nr
Match: KAG6584086.1 (hypothetical protein SDJN03_20018, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019687.1 hypothetical protein SDJN02_18650, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 152.9 bits (385), Expect = 1.7e-33
Identity = 82/117 (70.09%), Postives = 98/117 (83.76%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKH--GRGGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+++AGDDWGSF+SKH  GRGGNA+TS GD EK RLLG +K  ++    +
Sbjct: 1   MGNCCRSQSSTMIFAGDDWGSFSSKHGRGRGGNASTSSGDPEKYRLLG-EKQTSSCPQPQ 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPE 116
           VKIKMTK+ELEDLV+ LE +GLSLE+VIGRM    +EDE++ EHHRSWRPSLQSIPE
Sbjct: 61  VKIKMTKRELEDLVKNLEIEGLSLEQVIGRMT--NDEDEFQGEHHRSWRPSLQSIPE 114

[Close]

BLAST of Moc03g28400 vs. ExPASy TrEMBL
Match: A0A6J1CEB2 (uncharacterized protein LOC111010880 OS=Momordica charantia OX=3673 GN=LOC111010880 PE=4 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 8.2e-58
Identity = 117/117 (100.00%), Postives = 117/117 (100.00%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVK 60
           MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVK
Sbjct: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAAGEVK 60

Query: 61  IKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 118
           IKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY
Sbjct: 61  IKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 117

[Close]

BLAST of Moc03g28400 vs. ExPASy TrEMBL
Match: A0A5A7UTK3 (DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G005800 PE=4 SV=1)

HSP 1 Score: 174.1 bits (440), Expect = 3.4e-40
Identity = 92/119 (77.31%), Postives = 105/119 (88.24%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGR--GGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+V+AGDDWGSF+SKHGR  GGNATTSGGD EK RLLG +K AA+ +   
Sbjct: 1   MGNCCRSQSSTMVFAGDDWGSFSSKHGRGCGGNATTSGGDPEKHRLLGDQKEAASCSQPL 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPEDY 118
           VKIKMTK+ELE LV+KLE QGL+LE+VIGRM+KG  E+EYE+EHHRSWRPSLQSIPEDY
Sbjct: 61  VKIKMTKRELEVLVKKLEMQGLTLEQVIGRMMKG--EEEYEIEHHRSWRPSLQSIPEDY 117

[Close]

BLAST of Moc03g28400 vs. ExPASy TrEMBL
Match: A0A0A0LRL1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G063490 PE=4 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 6.7e-36
Identity = 88/121 (72.73%), Postives = 102/121 (84.30%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKHGRG----GNATTSGGDTEKQRLLGPKKAAATAAA 60
           MGNCCRSQSST+V+AGDDWGSF+SKHGRG     +A TSGGD EK RLLG +K A + + 
Sbjct: 1   MGNCCRSQSSTMVFAGDDWGSFSSKHGRGCGRNVSAATSGGDPEKHRLLG-EKEAGSCSQ 60

Query: 61  GEVKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPED 118
             VKIKMTK+ELE LV+KLE QGLSLE+VIGRM+KG  E+E+E+EHHRSWRPSLQSIPED
Sbjct: 61  PLVKIKMTKRELEVLVKKLEMQGLSLEQVIGRMMKG--EEEFEIEHHRSWRPSLQSIPED 118

[Close]

BLAST of Moc03g28400 vs. ExPASy TrEMBL
Match: A0A6J1EYG4 (uncharacterized protein LOC111440057 OS=Cucurbita moschata OX=3662 GN=LOC111440057 PE=4 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 6.3e-34
Identity = 82/117 (70.09%), Postives = 99/117 (84.62%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKH--GRGGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+++AGDDWGSF+SKH  GRGGNA+TS GD EK RLLG +K  ++    +
Sbjct: 1   MGNCCRSQSSTMIFAGDDWGSFSSKHGRGRGGNASTSSGDPEKYRLLG-EKQTSSCPQPQ 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPE 116
           VKIKMTK+ELEDLV+ LE +GLSLE+VIGRM+   +EDE++ EHHRSWRPSLQSIPE
Sbjct: 61  VKIKMTKRELEDLVKNLEIEGLSLEQVIGRMM--NDEDEFQGEHHRSWRPSLQSIPE 114

[Close]

BLAST of Moc03g28400 vs. ExPASy TrEMBL
Match: A0A6J1KIA5 (uncharacterized protein LOC111495485 OS=Cucurbita maxima OX=3661 GN=LOC111495485 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 3.1e-33
Identity = 82/117 (70.09%), Postives = 98/117 (83.76%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSFNSKH--GRGGNATTSGGDTEKQRLLGPKKAAATAAAGE 60
           MGNCCRSQSST+V+AGDDWGSF+SKH  GRGGNA+ S GD EK RLLG +K  ++    +
Sbjct: 1   MGNCCRSQSSTMVFAGDDWGSFSSKHGRGRGGNASASRGDPEKYRLLG-EKQTSSCPQPQ 60

Query: 61  VKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPE 116
           VKIKMTK+ELEDLV+ LE +GLSLE+VIGRM+   +EDE++ EHHRSWRPSLQSIPE
Sbjct: 61  VKIKMTKRELEDLVKNLEIEGLSLEQVIGRMM--NDEDEFQGEHHRSWRPSLQSIPE 114

[Close]

BLAST of Moc03g28400 vs. TAIR 10
Match: AT4G21920.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-terminal protein myristoylation; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G20340.1); Has 40 Blast hits to 40 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 40; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 73.6 bits (179), Expect = 1.2e-13
Identity = 43/127 (33.86%), Postives = 77/127 (60.63%), Query Frame = 0


Query: 1   MGNC-CRSQSSTLVWAGDDWGSFNSKHGRGGNATTSGGDTEKQRLLG------PKKAAAT 60
           MGNC C ++ +T  W+GDD GS+N +  R  +      + + ++LLG         ++++
Sbjct: 1   MGNCICVTEKTTTSWSGDDNGSYNKRRRRRRSTVVHDDNDDGEKLLGETSNVTSTSSSSS 60

Query: 61  AAAGEVKIKMTKKELEDLVEKLEKQGLSLEEVIGRMV-KGGEEDEYE----MEHHRSWRP 116
           +   E+KI++TKKELEDL+  +  + L+ EE++ +++ +GG++  +       HH+ W+P
Sbjct: 61  SERREIKIRITKKELEDLMRNIGLKSLTAEEILSKLIFEGGDQIGFSAVDVTNHHQPWKP 120

[Close]

BLAST of Moc03g28400 vs. TAIR 10
Match: AT3G20340.1 (Expression of the gene is downregulated in the presence of paraquat, an inducer of photoxidative stress. )

HSP 1 Score: 52.4 bits (124), Expect = 2.9e-07
Identity = 37/119 (31.09%), Postives = 57/119 (47.90%), Query Frame = 0


Query: 1   MGNCCRSQSSTLVWAGDDWGSF----NSKHGRGGNATTSGGDTEKQRLLGPKKAAATAAA 60
           MGNC R +S  + WAG+DW  F       H      T  G     + ++  + + ++  +
Sbjct: 1   MGNCLRHESE-MHWAGEDWDEFITEDEEDHHYSSKTTRDG-----KPVIVTRDSKSSVPS 60

Query: 61  GEVKIKMTKKELEDLVEKLEKQGLSLEEVIGRMVKGGEEDEYEMEHHRSWRPSLQSIPE 116
            E+KI++TKK+L DL+ K+    L+ ++              E    R WRP LQSIPE
Sbjct: 61  HEIKIRLTKKQLHDLLSKVNVHDLTFQQQTFSCPILNNRGYEEANQQRLWRPVLQSIPE 113

The following BLAST results are available for this feature:

BLAST of Moc03g28400 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140150.1	1.7e-57	100.00	uncharacterized protein LOC111010880 [Momordica charantia]	[more]
KAA0056859.1	7.1e-40	77.31	DUF4228 domain-containing protein [Cucumis melo var. makuwa] >TYJ99362.1 DUF4228...	[more]
XP_038895954.1	4.6e-39	76.47	uncharacterized protein LOC120084128 [Benincasa hispida]	[more]
XP_022933211.1	1.3e-33	70.09	uncharacterized protein LOC111440057 [Cucurbita moschata]	[more]
KAG6584086.1	1.7e-33	70.09	hypothetical protein SDJN03_20018, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Moc03g28400 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc03g28400 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CEB2	8.2e-58	100.00	uncharacterized protein LOC111010880 OS=Momordica charantia OX=3673 GN=LOC111010...	[more]
A0A5A7UTK3	3.4e-40	77.31	DUF4228 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A0A0LRL1	6.7e-36	72.73	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G063490 PE=4 SV=1	[more]
A0A6J1EYG4	6.3e-34	70.09	uncharacterized protein LOC111440057 OS=Cucurbita moschata OX=3662 GN=LOC1114400...	[more]
A0A6J1KIA5	3.1e-33	70.09	uncharacterized protein LOC111495485 OS=Cucurbita maxima OX=3661 GN=LOC111495485...	[more]

BLAST of Moc03g28400 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G21920.1	1.2e-13	33.86	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: N-termin...	[more]
AT3G20340.1	2.9e-07	31.09	Expression of the gene is downregulated in the presence of paraquat, an inducer ...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 58..85
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 21..45
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 26..40
None	No IPR available	PANTHER	PTHR33647:SF10	DOMAIN PROTEIN, PUTATIVE-RELATED	coord: 1..115
None	No IPR available	PANTHER	PTHR33647	OS01G0793900 PROTEIN	coord: 1..115

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g28400.1	Moc03g28400.1	mRNA