Moc03g27720 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g27720
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr3: 19924958 .. 19927877 (-)
RNA-Seq Expression	Moc03g27720
Synteny	Moc03g27720

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTTTCACTAAACAACATTGTAAGATGTGGCCCCTCCAAAATTAACGGAATCCGATCATTACTAGCGAGGCAATGGCACAATGAAGCAATTCCAGTTGCGTGGACAAGGCCCGAAATTGGATGGACAAAGCTGAATTTTGATGGGTCTTCCAAAGGGAAAAAATCACCAGGCAGAGGCAGCATTGGAGGCGTCCTGAGAGACCACGAGGCGCAGTTTCTGCTTGGATATGCAGAGCCCATCGGGCGAGCCAACAGCGCCGTGGCCGAACTCAGTGCCCTCGAAAAAGGGCTGGAATTGGTGATTGAAAATGGGTGGAAAGACGTTTGGGTTGAGGGAGATGCGAAGGGGTTGGTGGAAATAATAGTTGAAAACAGAGAGGTCAAATGCGTGGAAGCTCGAAGCCGTTTTCGGTACATAAATTCTATGATTCTGGATTTGGATAATTGCATGGTTAGTCATGTTTACAGGGAAGGTAATAAAGTTGCCCACAGCTTTGCTTCCATTGGGCATCGCTATAAGAAGCTTGAAATTTGGAGGGACATTCCCCCATTGCAGACTTTGGAGATGATGCGTCAAGATGCTGAGGGTAAGATTAGTTTTAGGAAAAAAACCACATCATAGTTGTACTTTTATTCACTTATTCGTTCTGTAAATTTTTTTTCTTTTTGCAAAATTTTATCCAGCTTGTATATATGGAATTAGTTGTAGCCACAGGGTGAAAATGATTTATGTATATAATATTGATCGATGTCGTCTTTCGTTTGTTATTGGGGATACTCGAGATTGTGTTTGTTTTAAATAAATTATGTGTGCTGGTATAATTATAACGTAAGTAAAGGGATAACATAATCCACATCTTTAAATTGACCATAAAATTCAAAATGACAGCCTCCTTAGTTATTACTAAGTTTGCGTGATTTACCCAAAATAGCCTCAAAAGAGTGAGAAAGTGTAGTACAAATATTTAGTTAAGTACAATGTAGTACAGAAGCAAGAATGCATATAGATTTATATTTGGAAAATGGGAAAATGGAAGGGAGTTTGATTAATAAATTAAAGGTGTGCAGTAGGTAGTACAAACAATAACCACTGTTGTACTTGTACTGCAACGCAAAGTCCACAGAAATTAAAAGAATGGTGTGTTATTTTATAAGGAGATGGAGTATAAATTCATGAGAATGAGCAAATAAAGTGGTGTGGGATTGAATTCGGTGGTAGTGGAGGCAGCACTACAATAGGCACAGTGAAGGGAGCTGTGTGATGAAGAAAAAGTCTTCAATTCCATCGCTTCGCTGTGGCTTTTGTAATATTGCCTTCCAACTCCCTCCTCCCTCCTCCCTCGCTCCTTCTTTGCCAAGTTTTCTGGGTCTCTCCTTGCTTCAAATTAAACTTGCCGGCCACCATGCCAGATTTTTTAAAAGCAATGACCAATTCAGCCTATTCGATCCATGGCGAATCGATTGCACCAATTTCCAAACCAACACATTTCCATTCTATAAATTGTTTCAAAGTCGATTGGGGGAGAGACGTTATTGTAAGGTCCCAATTTCACCAAAATGTAGGGACAGATATTTTATATTTAAATTGATAAATAAGTATTTTACTATTTTTTTCTTCTAAATGTTTAATATTAATATTAGAAATGTAGGAGACGAAAATTTAATATTTTGGGTACAAGTAGATGAACTTTTTATCAATGTATATAATGTAATTGGATTGTGCGGGCCTTTTCTTTTCCGTTCAGTTTTGTTATACTAGTGCTACTTGCTTCTGAACTGAGAACTCAAAGATATAAATGGAAATAATTCTATTTTTATTGAATAATTAATATATTGCTCGCTTAATTCTAATCGACTAAACATGAATGAGATAAATAATTTAAGGGACGTTAGATAACCGTTTCCTTTTTTATTTTTGAAAAACAGACCTGTCGATTCAATTTTTTACTTTCTGACTTTTAAAAAAGTGTTTGAAAATCTAAATAAATGTTCTTCTATAAAAACAGTTTTTTAAAAAATAATTTTTGAAAGCAAATTTCTTTTTTTTCCCCTCAATTTTTGTTACCTAGTAAATTTATTTCTCACTCTAAAAATTTTTTCAGACCTTAAATTTCTCTTTCTAAAAATTTTATCTTTCTCCCTCTAAAATTTTTATTTTTATTATATTTTTATTTATTTCTTTTTTCTAACTTATCATTTTCAATCTACCCATCCTAAAAATTTAATATTTTCCAAAAATTTTTACATTTAAATTTTATCTTTTATAATTTCTGTTTCTCTTTCTAATTTTTTTTTTTAAGAAAATATGTCTCCAACCTTTTTTTTTTTATCAAAATCCTTTTCGGGTTCAATTTTATTTCACTATAATTTTTCTCTCTTTTAATTTATTTAGTTTCTCTAACAAACATATATAACTTTTTTTTTAAAATTAGTTTCTTATTATCATTTTTATTTTTCTTTCTCTTATTTTGCTCTCAAATTTACTATCACTAAAATTTTCAATTTTTGTCTCTAATTTTTTTTTCTTTTCCCTTGCTAAATTTACTCTATCTAAAATTTATCTTTAAAAAATTTCTTTCAATCTCTCTGAACTTTTATTTTCACGTGTCTACTTGAGGGATGACAAAAATTCCCTCAAGGTTGGGTCCCCGCTCCAAGAACATGATACGCATTTCCCACGACCTCCCAAACTCTATCCTTGATTCCATTTTTTAAATTATTAATATATTGTTGTTACCATGATAAAATTTTAGCAATGATTAAATGTGTCGTCAGTATTTTTTAATTATATTTAGGATCAATCACAAATTTATTTATGTCTTAAATTAATTATAAAATCGGAAAGTCAAATTATGCCACATGTCTTTTTTTATGATCGATGGTATCAACTAATTTGATCAACCAATGTAGAAATTTGA

mRNA sequence

ATGTTTTCACTAAACAACATTGTAAGATGTGGCCCCTCCAAAATTAACGGAATCCGATCATTACTAGCGAGGCAATGGCACAATGAAGCAATTCCAGTTGCGTGGACAAGGCCCGAAATTGGATGGACAAAGCTGAATTTTGATGGGTCTTCCAAAGGGAAAAAATCACCAGGCAGAGGCAGCATTGGAGGCGTCCTGAGAGACCACGAGGCGCAGTTTCTGCTTGGATATGCAGAGCCCATCGGGCGAGCCAACAGCGCCGTGGCCGAACTCAGTGCCCTCGAAAAAGGGCTGGAATTGGTGATTGAAAATGGGTGGAAAGACGTTTGGGTTGAGGGAGATGCGAAGGGGTTGGTGGAAATAATAGTTGAAAACAGAGAGGTCAAATGCGTGGAAGCTCGAAGCCGTTTTCGGTACATAAATTCTATGATTCTGGATTTGGATAATTGCATGGTTAGTCATGTTTACAGGGAAGGTAATAAAGTTGCCCACAGCTTTGCTTCCATTGGGCATCGCTATAAGAAGCTTGAAATTTGGAGGGACATTCCCCCATTGCAGACTTTGGAGATGATGCGTCAAGATGCTGAGGAAATTTGA

Coding sequence (CDS)

ATGTTTTCACTAAACAACATTGTAAGATGTGGCCCCTCCAAAATTAACGGAATCCGATCATTACTAGCGAGGCAATGGCACAATGAAGCAATTCCAGTTGCGTGGACAAGGCCCGAAATTGGATGGACAAAGCTGAATTTTGATGGGTCTTCCAAAGGGAAAAAATCACCAGGCAGAGGCAGCATTGGAGGCGTCCTGAGAGACCACGAGGCGCAGTTTCTGCTTGGATATGCAGAGCCCATCGGGCGAGCCAACAGCGCCGTGGCCGAACTCAGTGCCCTCGAAAAAGGGCTGGAATTGGTGATTGAAAATGGGTGGAAAGACGTTTGGGTTGAGGGAGATGCGAAGGGGTTGGTGGAAATAATAGTTGAAAACAGAGAGGTCAAATGCGTGGAAGCTCGAAGCCGTTTTCGGTACATAAATTCTATGATTCTGGATTTGGATAATTGCATGGTTAGTCATGTTTACAGGGAAGGTAATAAAGTTGCCCACAGCTTTGCTTCCATTGGGCATCGCTATAAGAAGCTTGAAATTTGGAGGGACATTCCCCCATTGCAGACTTTGGAGATGATGCGTCAAGATGCTGAGGAAATTTGA

Protein sequence

MFSLNNIVRCGPSKINGIRSLLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWRDIPPLQTLEMMRQDAEEI

Homology

[Close]

BLAST of Moc03g27720 vs. NCBI nr
Match: KAG6584039.1 (putative ribonuclease H protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 326.6 bits (836), Expect = 1.4e-85
Identity = 152/197 (77.16%), Postives = 178/197 (90.36%), Query Frame = 0


Query: 1   MFSLNNIVRCGPSKINGIRS-LLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPGR 60
           MFSLN +++C   +IN IR+ LLARQ H + IPVAW RP+IGWTKLNFDGSS+GK SPGR
Sbjct: 1   MFSLNTMLKCPNYQINRIRALLLARQCHKQPIPVAWKRPDIGWTKLNFDGSSRGKMSPGR 60

Query: 61  GSIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLV 120
            SIGGVLRDH+AQFLLGYAEPIGRANS +AEL+AL KGLELV+ENGWKDVW+EGDAKGL+
Sbjct: 61  ASIGGVLRDHQAQFLLGYAEPIGRANSTIAELTALSKGLELVLENGWKDVWLEGDAKGLI 120

Query: 121 EIIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIW 180
           EIIV  ++ KCVEARS+FRYINS+ILDL+NC VSH+YREGN+VA+ FASIGHRYKKLE+W
Sbjct: 121 EIIVGKKKAKCVEARSQFRYINSLILDLNNCKVSHIYREGNRVANRFASIGHRYKKLEVW 180

Query: 181 RDIPPLQTLEMMRQDAE 197
           RDIPPL+TL+MMR+DAE
Sbjct: 181 RDIPPLETLDMMREDAE 197

[Close]

BLAST of Moc03g27720 vs. NCBI nr
Match: KAA0056936.1 (putative ribonuclease H protein [Cucumis melo var. makuwa] >TYK26364.1 putative ribonuclease H protein [Cucumis melo var. makuwa])

HSP 1 Score: 297.4 bits (760), Expect = 9.4e-77
Identity = 142/178 (79.78%), Postives = 157/178 (88.20%), Query Frame = 0


Query: 21  LLARQWHNE--AIPVAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYA 80
           L ARQ H +   IPVAW RPEIGWTKLNFDGSSKG K+PG  SIGGVLRDH+AQFLLGYA
Sbjct: 19  LAARQLHKQPKPIPVAWRRPEIGWTKLNFDGSSKGDKAPGLASIGGVLRDHKAQFLLGYA 78

Query: 81  EPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFR 140
           E IGRANSA+AEL+AL KGLELV+ENGWKDVWVEGD KGLVEI+ +N+EVKCVE RS FR
Sbjct: 79  EAIGRANSAMAELTALTKGLELVLENGWKDVWVEGDDKGLVEILADNKEVKCVETRSYFR 138

Query: 141 YINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWRDIPPLQTLEMMRQDAE 197
           YI S+ILDLDNC VSH+YREGNKVA  FASIGHRYKKLEIWR++PPL+TL+MMR DAE
Sbjct: 139 YIKSLILDLDNCKVSHIYREGNKVADRFASIGHRYKKLEIWRELPPLETLDMMRHDAE 196

[Close]

BLAST of Moc03g27720 vs. NCBI nr
Match: KAE8652698.1 (hypothetical protein Csa_013405 [Cucumis sativus])

HSP 1 Score: 283.5 bits (724), Expect = 1.4e-72
Identity = 136/178 (76.40%), Postives = 152/178 (85.39%), Query Frame = 0


Query: 21  LLARQWHNE--AIPVAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYA 80
           L AR+ H     IPVAWTRPE GWTKLNFDGSSKG+  PG  SIGGVLRDH+AQFLLGYA
Sbjct: 20  LAARELHKRPIPIPVAWTRPEFGWTKLNFDGSSKGEIGPGVASIGGVLRDHKAQFLLGYA 79

Query: 81  EPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFR 140
           E IGRA S++AEL AL KGLELV+ENGWKDVWVEGDAKGLVEI+ ENREVKC+EARS  R
Sbjct: 80  ESIGRAYSSMAELKALTKGLELVLENGWKDVWVEGDAKGLVEILAENREVKCMEARSYLR 139

Query: 141 YINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWRDIPPLQTLEMMRQDAE 197
           +I S++LD DNC VSH+YREGNKVA  FASIGHR KKLEIWR++PPL+TL+MMR DAE
Sbjct: 140 HIKSLLLDFDNCKVSHIYREGNKVADRFASIGHRCKKLEIWRELPPLETLDMMRHDAE 197

[Close]

BLAST of Moc03g27720 vs. NCBI nr
Match: XP_038895327.1 (uncharacterized protein LOC120083578 [Benincasa hispida])

HSP 1 Score: 279.3 bits (713), Expect = 2.7e-71
Identity = 140/196 (71.43%), Postives = 154/196 (78.57%), Query Frame = 0


Query: 1   MFSLNNIVRCGPSKINGIRSLLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPGRG 60
           MF+L  I++C   +I  IRS LARQWH + IPVAWTRPEIGWTKLNFDGSSKGKK+PGR 
Sbjct: 1   MFTLKTILKCSNYQI--IRSSLARQWHKQPIPVAWTRPEIGWTKLNFDGSSKGKKTPGRA 60

Query: 61  SIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVE 120
           SIGGVLRDH+AQFLLGYAEPIGRANS +AEL+AL KGLELV+ENGWKDVWVEGDAKGLVE
Sbjct: 61  SIGGVLRDHDAQFLLGYAEPIGRANSTMAELTALTKGLELVLENGWKDVWVEGDAKGLVE 120

Query: 121 IIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWR 180
           IIVEN+EVKCVEARS FR                           FASIGHRYKKLEIWR
Sbjct: 121 IIVENKEVKCVEARSHFR---------------------------FASIGHRYKKLEIWR 167

Query: 181 DIPPLQTLEMMRQDAE 197
           +IPPL+ L+MMRQDAE
Sbjct: 181 EIPPLEILDMMRQDAE 167

[Close]

BLAST of Moc03g27720 vs. NCBI nr
Match: KAG5516709.1 (hypothetical protein RHGRI_037449 [Rhododendron griersonianum])

HSP 1 Score: 247.7 bits (631), Expect = 8.6e-62
Identity = 115/196 (58.67%), Postives = 143/196 (72.96%), Query Frame = 0


Query: 1   MFSLNNIVRCGPSKINGIRSLLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPGRG 60
           M ++ NI+RC     +  R  + R WH E I VAW +PEIGWTKLNFDGS KGK   G+ 
Sbjct: 1   MLTIPNIMRCQKLTQSPFRLAIIRNWHKEPIQVAWEKPEIGWTKLNFDGSCKGK--TGKA 60

Query: 61  SIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVE 120
           SIGGV R+H+A+FLLGYAE IGR  S +AEL+AL +GLELV+ENGW+DVW+EGD+K LVE
Sbjct: 61  SIGGVFRNHKAEFLLGYAEAIGRTTSTIAELAALRRGLELVLENGWEDVWLEGDSKALVE 120

Query: 121 IIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWR 180
           II   + VKC EA+    +IN MI +L+NCM+SHVYREGNK A  FA +GH+ KK ++WR
Sbjct: 121 IIARRKRVKCAEAQGHISHINLMIPELNNCMLSHVYREGNKAADKFAQMGHQMKKPQVWR 180

Query: 181 DIPPLQTLEMMRQDAE 197
             PP Q L +M  DAE
Sbjct: 181 HNPPHQVLRIMHDDAE 194

[Close]

BLAST of Moc03g27720 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.9e-09
Identity = 43/139 (30.94%), Postives = 68/139 (48.92%), Query Frame = 0


Query: 33  VAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYAEPIGRANSAVAELS 92
           + W  P +GW K+N DG+S+G  +PG  S GGVLRD    +  G++  IGR ++  AEL 
Sbjct: 450 IGWVSPCVGWVKVNTDGASRG--NPGLASAGGVLRDCTGAWCGGFSLNIGRCSAPQAELW 509

Query: 93  ALEKGLELVIENGWKDVWVEGDAK---GLVEIIVENREVKCVEARSRFRYINSMILDLDN 152
            +  GL    E     V +E D++   G ++  + +        R    ++    L    
Sbjct: 510 GVYYGLYFAWEKKVPRVELEVDSEVIVGFLKTGISDSHPLSFLVRLCHGFLQKDWL---- 569

Query: 153 CMVSHVYREGNKVAHSFAS 169
             + HVYRE N++A   A+
Sbjct: 570 VRIVHVYREANRLADGLAN 582

[Close]

BLAST of Moc03g27720 vs. ExPASy TrEMBL
Match: A0A5D3DS33 (Putative ribonuclease H protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00240 PE=4 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 4.6e-77
Identity = 142/178 (79.78%), Postives = 157/178 (88.20%), Query Frame = 0


Query: 21  LLARQWHNE--AIPVAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYA 80
           L ARQ H +   IPVAW RPEIGWTKLNFDGSSKG K+PG  SIGGVLRDH+AQFLLGYA
Sbjct: 19  LAARQLHKQPKPIPVAWRRPEIGWTKLNFDGSSKGDKAPGLASIGGVLRDHKAQFLLGYA 78

Query: 81  EPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFR 140
           E IGRANSA+AEL+AL KGLELV+ENGWKDVWVEGD KGLVEI+ +N+EVKCVE RS FR
Sbjct: 79  EAIGRANSAMAELTALTKGLELVLENGWKDVWVEGDDKGLVEILADNKEVKCVETRSYFR 138

Query: 141 YINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWRDIPPLQTLEMMRQDAE 197
           YI S+ILDLDNC VSH+YREGNKVA  FASIGHRYKKLEIWR++PPL+TL+MMR DAE
Sbjct: 139 YIKSLILDLDNCKVSHIYREGNKVADRFASIGHRYKKLEIWRELPPLETLDMMRHDAE 196

[Close]

BLAST of Moc03g27720 vs. ExPASy TrEMBL
Match: A0A0A0LUF5 (RNase H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G071820 PE=4 SV=1)

HSP 1 Score: 283.5 bits (724), Expect = 6.8e-73
Identity = 136/178 (76.40%), Postives = 152/178 (85.39%), Query Frame = 0


Query: 21  LLARQWHNE--AIPVAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYA 80
           L AR+ H     IPVAWTRPE GWTKLNFDGSSKG+  PG  SIGGVLRDH+AQFLLGYA
Sbjct: 20  LAARELHKRPIPIPVAWTRPEFGWTKLNFDGSSKGEIGPGVASIGGVLRDHKAQFLLGYA 79

Query: 81  EPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFR 140
           E IGRA S++AEL AL KGLELV+ENGWKDVWVEGDAKGLVEI+ ENREVKC+EARS  R
Sbjct: 80  ESIGRAYSSMAELKALTKGLELVLENGWKDVWVEGDAKGLVEILAENREVKCMEARSYLR 139

Query: 141 YINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWRDIPPLQTLEMMRQDAE 197
           +I S++LD DNC VSH+YREGNKVA  FASIGHR KKLEIWR++PPL+TL+MMR DAE
Sbjct: 140 HIKSLLLDFDNCKVSHIYREGNKVADRFASIGHRCKKLEIWRELPPLETLDMMRHDAE 197

[Close]

BLAST of Moc03g27720 vs. ExPASy TrEMBL
Match: A0A7N2LU47 (RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 244.6 bits (623), Expect = 3.5e-61
Identity = 116/197 (58.88%), Postives = 147/197 (74.62%), Query Frame = 0


Query: 1   MFSLNNIVRCGPSKINGIR-SLLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPGR 60
           M S+ NI+RC       I   +L R WH EAIPV W +PE GWTKLNFDGSSKGK +  +
Sbjct: 1   MLSITNILRCQIPITKQISVRILIRNWHKEAIPVVWEKPEKGWTKLNFDGSSKGKAN--K 60

Query: 61  GSIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLV 120
            SIGGV R+H+A+F+LGYAE IGRANS +AEL+AL +GLELV+ENGW DVW+EGDAK L+
Sbjct: 61  ASIGGVFRNHKAEFILGYAESIGRANSTIAELAALRRGLELVLENGWNDVWLEGDAKTLI 120

Query: 121 EIIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIW 180
           +II + R+VK  E +   R IN +IL+LDNC+V+HVYREGN+ A  FA IGH  +K  IW
Sbjct: 121 DIIAKRRQVKSSEVQRHIRDINLIILELDNCLVTHVYREGNRAADKFAQIGHHMEKPGIW 180

Query: 181 RDIPPLQTLEMMRQDAE 197
           R++PP + L +M+ DAE
Sbjct: 181 RNVPPDELLAIMKDDAE 195

[Close]

BLAST of Moc03g27720 vs. ExPASy TrEMBL
Match: A0A2N9IF10 (RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS50940 PE=4 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 1.0e-60
Identity = 116/198 (58.59%), Postives = 148/198 (74.75%), Query Frame = 0


Query: 1   MFSLNNIVRCGPSKI--NGIRSLLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPG 60
           M S+ NI+RC    I  N +R  L R WH EAIPV W +PEIGWTKLNFDGSSKG+ +  
Sbjct: 1   MLSITNILRCQSRPILQNSVRFFL-RNWHKEAIPVTWEKPEIGWTKLNFDGSSKGRAN-- 60

Query: 61  RGSIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGL 120
           + SIGGV R+H+A+FLLGYAE IG+ANS +AEL+AL +GLELV+ENGW DVW+EGDAK L
Sbjct: 61  KASIGGVFRNHKAEFLLGYAESIGKANSTIAELAALRRGLELVLENGWNDVWLEGDAKTL 120

Query: 121 VEIIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEI 180
           ++IIV+ R VKC+E +     IN ++L+LDN  V+HVYREGN+ A  FA IGH  K   I
Sbjct: 121 IDIIVQGRRVKCLEVQRHISDINLILLELDNFHVTHVYREGNRAADKFAQIGHHMKNPGI 180

Query: 181 WRDIPPLQTLEMMRQDAE 197
           WR+ PP + L +M++DA+
Sbjct: 181 WREDPPDELLAIMKEDAQ 195

[Close]

BLAST of Moc03g27720 vs. ExPASy TrEMBL
Match: A0A061GEZ6 (Polynucleotidyl transferase OS=Theobroma cacao OX=3641 GN=TCM_030014 PE=4 SV=1)

HSP 1 Score: 240.7 bits (613), Expect = 5.1e-60
Identity = 112/196 (57.14%), Postives = 148/196 (75.51%), Query Frame = 0


Query: 1   MFSLNNIVRCGPSKINGIRSLLARQWHNEAIPVAWTRPEIGWTKLNFDGSSKGKKSPGRG 60
           M + +NI RC     N ++ LL R WH EAIPV+W +PEIGWTKLNFDGS KG+   G+ 
Sbjct: 2   MLAFSNIFRCQRLIQNPVQ-LLIRNWHKEAIPVSWEKPEIGWTKLNFDGSCKGR--GGKA 61

Query: 61  SIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIENGWKDVWVEGDAKGLVE 120
           SIGGV R+H+A+FLLGYAE IGR+ S +AEL+AL +GLELV+ENGW DVW+EGDAK LV+
Sbjct: 62  SIGGVFRNHKAEFLLGYAESIGRSTSTIAELAALRRGLELVLENGWTDVWLEGDAKTLVD 121

Query: 121 IIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNKVAHSFASIGHRYKKLEIWR 180
           +IV+ R+VKC E +    +IN +I +L+NC+V+H+YREGN+ A   A IGH+ KK +IW 
Sbjct: 122 VIVQRRQVKCAELQRHVSHINLIIPELNNCIVTHIYREGNRAADKLAQIGHQLKKPQIWW 181

Query: 181 DIPPLQTLEMMRQDAE 197
            IPP + L ++ +DAE
Sbjct: 182 HIPPNEVLPIVHEDAE 194

[Close]

BLAST of Moc03g27720 vs. TAIR 10
Match: AT5G42905.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 1.1e-09
Identity = 34/87 (39.08%), Postives = 50/87 (57.47%), Query Frame = 0


Query: 33  VAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYAEPIGRANSAVAELS 92
           + W  P +GW K+N DG+S+G  +PG  S GGVLRD E  +  G++  IGR ++  AEL 
Sbjct: 138 IGWVLPCVGWVKVNTDGASRG--NPGLASAGGVLRDCEGAWCGGFSLNIGRCSAQHAELW 197

Query: 93  ALEKGLELVIENGWKDVWVEGDAKGLV 120
            +  GL    E     V +E D++ +V
Sbjct: 198 GVYYGLYFAWEKKVPRVELEVDSEAIV 222

[Close]

BLAST of Moc03g27720 vs. TAIR 10
Match: AT2G46460.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 60.1 bits (144), Expect = 2.4e-09
Identity = 39/136 (28.68%), Postives = 66/136 (48.53%), Query Frame = 0


Query: 33  VAWTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFL-LGYAEPIGRANSAVAEL 92
           + WTRP  GW K N+DG S  ++ P +   G ++RD   QF+  G A     +N+  +E 
Sbjct: 1   MGWTRPPHGWKKCNYDG-SYNREMPSKA--GWIIRDESGQFVSAGQATGNHTSNALESEF 60

Query: 93  SALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFRYINSMILDLDNCM 152
            AL   ++    +G + +W EGD + ++EI+  NR     +  +  R + +       C 
Sbjct: 61  QALLITMQSCWSHGHRKIWFEGDNREVMEIL--NRRKSRFDIFNWIRDVQAWKQRFQECR 120

Query: 153 VSHVYREGNKVAHSFA 168
            + + R+ NK A   A
Sbjct: 121 FTWINRKQNKPADILA 131

[Close]

BLAST of Moc03g27720 vs. TAIR 10
Match: AT1G04625.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 2.6e-08
Identity = 42/135 (31.11%), Postives = 60/135 (44.44%), Query Frame = 0


Query: 35  WTRPEIGWTKLNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYAEPIG--RANSAVAELS 94
           WT P IGWTK N+DG+     S      G +LRD    F LG A  IG    N   +EL 
Sbjct: 82  WTTPPIGWTKCNYDGT---YHSNAPSKAGWLLRDDRGTF-LGAAHAIGSITTNPMESELQ 141

Query: 95  ALEKGLELVIENGWKDVWVEGDAKGLVEIIVENREVKCVEARSRFRYINSMILDLDNCMV 154
           AL   ++     G++ ++ EGD K + EI+  N         +  R I++     + C  
Sbjct: 142 ALVMAMQHCWSRGYRKIYFEGDNKEVSEIV--NGRSSNFAVFNWIRDISAWRSKFEECKF 201

Query: 155 SHVYREGNKVAHSFA 168
           +   R  N  A + A
Sbjct: 202 TWTRRFSNMAADALA 210

[Close]

BLAST of Moc03g27720 vs. TAIR 10
Match: AT3G01410.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 39/127 (30.71%), Postives = 61/127 (48.03%), Query Frame = 0


Query: 45  LNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIEN 104
           + FDG+SKG  +PG+   G VLR  +   L    E +G A + VAE  AL  GL   ++ 
Sbjct: 157 IEFDGASKG--NPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDK 216

Query: 105 GWKDVWVEGDAKGL---VEIIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNK 164
           G+K+V V GD+  +   V+   +    K  E   + + + +     D   + H+ RE N 
Sbjct: 217 GFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFD---IKHIAREKNS 276

Query: 165 VAHSFAS 169
            A   A+
Sbjct: 277 EADKQAN 278

[Close]

BLAST of Moc03g27720 vs. TAIR 10
Match: AT3G01410.2 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 53.5 bits (127), Expect = 2.2e-07
Identity = 39/127 (30.71%), Postives = 61/127 (48.03%), Query Frame = 0


Query: 45  LNFDGSSKGKKSPGRGSIGGVLRDHEAQFLLGYAEPIGRANSAVAELSALEKGLELVIEN 104
           + FDG+SKG  +PG+   G VLR  +   L    E +G A + VAE  AL  GL   ++ 
Sbjct: 157 IEFDGASKG--NPGKAGAGAVLRASDNSVLFYLREGVGNATNNVAEYRALLLGLRSALDK 216

Query: 105 GWKDVWVEGDAKGL---VEIIVENREVKCVEARSRFRYINSMILDLDNCMVSHVYREGNK 164
           G+K+V V GD+  +   V+   +    K  E   + + + +     D   + H+ RE N 
Sbjct: 217 GFKNVHVLGDSMLVCMQVQGAWKTNHPKMAELCKQAKELMNSFKTFD---IKHIAREKNS 276

Query: 165 VAHSFAS 169
            A   A+
Sbjct: 277 EADKQAN 278

The following BLAST results are available for this feature:

BLAST of Moc03g27720 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6584039.1	1.4e-85	77.16	putative ribonuclease H protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAA0056936.1	9.4e-77	79.78	putative ribonuclease H protein [Cucumis melo var. makuwa] >TYK26364.1 putative ...	[more]
KAE8652698.1	1.4e-72	76.40	hypothetical protein Csa_013405 [Cucumis sativus]	[more]
XP_038895327.1	2.7e-71	71.43	uncharacterized protein LOC120083578 [Benincasa hispida]	[more]
KAG5516709.1	8.6e-62	58.67	hypothetical protein RHGRI_037449 [Rhododendron griersonianum]	[more]

BLAST of Moc03g27720 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P0C2F6	3.9e-09	30.94	Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1...	[more]

BLAST of Moc03g27720 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3DS33	4.6e-77	79.78	Putative ribonuclease H protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A0A0LUF5	6.8e-73	76.40	RNase H domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G071820 PE=...	[more]
A0A7N2LU47	3.5e-61	58.88	RNase H domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]
A0A2N9IF10	1.0e-60	58.59	RNase H domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS50940 ...	[more]
A0A061GEZ6	5.1e-60	57.14	Polynucleotidyl transferase OS=Theobroma cacao OX=3641 GN=TCM_030014 PE=4 SV=1	[more]

BLAST of Moc03g27720 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G42905.1	1.1e-09	39.08	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G46460.1	2.4e-09	28.68	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G04625.1	2.6e-08	31.11	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G01410.1	2.2e-07	30.71	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT3G01410.2	2.2e-07	30.71	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 46..168 e-value: 2.6E-28 score: 98.3
IPR002156	Ribonuclease H domain	PROSITE	PS50879	RNASE_H	coord: 39..172 score: 9.685842
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 42..171 e-value: 6.1E-21 score: 76.8
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 15..196
None	No IPR available	PANTHER	PTHR47723:SF3	OS06G0683500 PROTEIN	coord: 15..196
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 45..167 e-value: 4.61816E-28 score: 100.081
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 40..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g27720.1	Moc03g27720.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity