Moc03g27500 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g27500
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	alpha/beta-Hydrolases superfamily protein
Location	chr3: 19791465 .. 19792436 (+)
RNA-Seq Expression	Moc03g27500
Synteny	Moc03g27500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATGAAGAACAAGAAGAAGAAGAAAATCGCAAACCCTTTCTCAATTACTGGGGAAACACCCCCGAGGAAGAATATTTCGCATCCCAACGAATCAAAGCCTCGAAATCCTTCTACACTTCCCCTCGCGGCCTCAAGCTCTTCACTCGTTCATGGCTTCCCCTCCCTCCCGCCCCTCCCCGAGCCCTGATCTTCATGGTCCATGGCTACGGCAACAACATCAGCTGGACTTTCCAAGCAACCTCCATCTTCCTCGCCCAATCCGGCTTCGCTTGCTTCGCCCTTGACCTCGAAGGCCATGGCGCATCCCAAGGCCTCAAGGCTTTCGTCCCCAATGTTGACTCTGTAGTCCACGACTGCCTCTGCTTCTTCAATTTTGTCAAACAGGACCCCCAATTTCAGGGCCTGCCCTCGTTCCTCTACGGCGAGTCCATGGGCGGCGCGATTTGCCTCTTGATTCATTTCGCCGATCCTCTAGGGTTTGACGGCGCTGTACTCGTCGCGCCCATGTGCAGAATCTCCGATAACGTGAAACCGCGTTGGCCGATTCCTCAATTTCTGACGGCGGTTGCGAAATTTTTGCCCACTCTGGCGATCGTCCCGACGGCGGATCTTCTGGACAAATCGGTGAAGGTGGAGGAGAAGAAAATCGTGGCGGAGATGAATCCGATGAGGTATAGGGGAAAACCCAGATTGGGAACCGTGTTGGAGCTTCTTAGAGTGACGGAGCATTTGAGCCAGAGACTGAGCGATGTGAGGCTTCCATTCATCGTCCTCCATGGAAATGCGGATGTTGTTACGGACCCGAGTGTGAGTAAGGCTCTGTATGAAGAGGCGAAGAGCGAGGACAAGACGATCAAAATTTACGAGGGCATGATGCATTCTATGTTGTTTGGGGAGACTGATGAGAATGTGGAAACTGTTCGAAATGACATTCTTGCTTGGTTTAATGGAAGATGCAGGAGAAAGTGA

mRNA sequence

ATGAATGAAGAACAAGAAGAAGAAGAAAATCGCAAACCCTTTCTCAATTACTGGGGAAACACCCCCGAGGAAGAATATTTCGCATCCCAACGAATCAAAGCCTCGAAATCCTTCTACACTTCCCCTCGCGGCCTCAAGCTCTTCACTCGTTCATGGCTTCCCCTCCCTCCCGCCCCTCCCCGAGCCCTGATCTTCATGGTCCATGGCTACGGCAACAACATCAGCTGGACTTTCCAAGCAACCTCCATCTTCCTCGCCCAATCCGGCTTCGCTTGCTTCGCCCTTGACCTCGAAGGCCATGGCGCATCCCAAGGCCTCAAGGCTTTCGTCCCCAATGTTGACTCTGTAGTCCACGACTGCCTCTGCTTCTTCAATTTTGTCAAACAGGACCCCCAATTTCAGGGCCTGCCCTCGTTCCTCTACGGCGAGTCCATGGGCGGCGCGATTTGCCTCTTGATTCATTTCGCCGATCCTCTAGGGTTTGACGGCGCTGTACTCGTCGCGCCCATGTGCAGAATCTCCGATAACGTGAAACCGCGTTGGCCGATTCCTCAATTTCTGACGGCGGTTGCGAAATTTTTGCCCACTCTGGCGATCGTCCCGACGGCGGATCTTCTGGACAAATCGGTGAAGGTGGAGGAGAAGAAAATCGTGGCGGAGATGAATCCGATGAGGTATAGGGGAAAACCCAGATTGGGAACCGTGTTGGAGCTTCTTAGAGTGACGGAGCATTTGAGCCAGAGACTGAGCGATGTGAGGCTTCCATTCATCGTCCTCCATGGAAATGCGGATGTTGTTACGGACCCGAGTGTGAGTAAGGCTCTGTATGAAGAGGCGAAGAGCGAGGACAAGACGATCAAAATTTACGAGGGCATGATGCATTCTATGTTGTTTGGGGAGACTGATGAGAATGTGGAAACTGTTCGAAATGACATTCTTGCTTGGTTTAATGGAAGATGCAGGAGAAAGTGA

Coding sequence (CDS)

ATGAATGAAGAACAAGAAGAAGAAGAAAATCGCAAACCCTTTCTCAATTACTGGGGAAACACCCCCGAGGAAGAATATTTCGCATCCCAACGAATCAAAGCCTCGAAATCCTTCTACACTTCCCCTCGCGGCCTCAAGCTCTTCACTCGTTCATGGCTTCCCCTCCCTCCCGCCCCTCCCCGAGCCCTGATCTTCATGGTCCATGGCTACGGCAACAACATCAGCTGGACTTTCCAAGCAACCTCCATCTTCCTCGCCCAATCCGGCTTCGCTTGCTTCGCCCTTGACCTCGAAGGCCATGGCGCATCCCAAGGCCTCAAGGCTTTCGTCCCCAATGTTGACTCTGTAGTCCACGACTGCCTCTGCTTCTTCAATTTTGTCAAACAGGACCCCCAATTTCAGGGCCTGCCCTCGTTCCTCTACGGCGAGTCCATGGGCGGCGCGATTTGCCTCTTGATTCATTTCGCCGATCCTCTAGGGTTTGACGGCGCTGTACTCGTCGCGCCCATGTGCAGAATCTCCGATAACGTGAAACCGCGTTGGCCGATTCCTCAATTTCTGACGGCGGTTGCGAAATTTTTGCCCACTCTGGCGATCGTCCCGACGGCGGATCTTCTGGACAAATCGGTGAAGGTGGAGGAGAAGAAAATCGTGGCGGAGATGAATCCGATGAGGTATAGGGGAAAACCCAGATTGGGAACCGTGTTGGAGCTTCTTAGAGTGACGGAGCATTTGAGCCAGAGACTGAGCGATGTGAGGCTTCCATTCATCGTCCTCCATGGAAATGCGGATGTTGTTACGGACCCGAGTGTGAGTAAGGCTCTGTATGAAGAGGCGAAGAGCGAGGACAAGACGATCAAAATTTACGAGGGCATGATGCATTCTATGTTGTTTGGGGAGACTGATGAGAATGTGGAAACTGTTCGAAATGACATTCTTGCTTGGTTTAATGGAAGATGCAGGAGAAAGTGA

Protein sequence

MNEEQEEEENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFNGRCRRK

Homology

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BLAST of Moc03g27500 vs. NCBI nr
Match: XP_023001731.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 593.2 bits (1528), Expect = 1.4e-165
Identity = 288/323 (89.16%), Postives = 303/323 (93.81%), Query Frame = 0


Query: 1   MNEEQEEE--ENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPA 60
           MNE++ E+   + + FLNYWGNT EE+YFASQRIKASKSFYTSPRG KLFTRSWLPLPP 
Sbjct: 12  MNEQESEQGHGHGRSFLNYWGNTSEEDYFASQRIKASKSFYTSPRGFKLFTRSWLPLPPT 71

Query: 61  PPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVH 120
           PPRALIFMVHGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVH
Sbjct: 72  PPRALIFMVHGYGNNISWTFQATSIFLAQKGFACFALDLEGHGRSQGLKAFVPNVDSVVH 131

Query: 121 DCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVK 180
           DCL FFNF+K DPQFQGLPSFLYGESMGGAICLLIHFADP GFDGA+LVAPMC+ISDNVK
Sbjct: 132 DCLSFFNFLKLDPQFQGLPSFLYGESMGGAICLLIHFADPKGFDGAILVAPMCKISDNVK 191

Query: 181 PRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLEL 240
           P WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+EL
Sbjct: 192 PNWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVEL 251

Query: 241 LRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLF 300
           LRVTEHLSQRL DV LPFIVLHGNADVVTDP+VSK LYEEAKSEDKTIKIYEGMMHS+L+
Sbjct: 252 LRVTEHLSQRLRDVTLPFIVLHGNADVVTDPNVSKTLYEEAKSEDKTIKIYEGMMHSLLY 311

Query: 301 GETDENVETVRNDILAWFNGRCR 322
           GE+DENVE VRNDIL+W NGRCR
Sbjct: 312 GESDENVEIVRNDILSWLNGRCR 334

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BLAST of Moc03g27500 vs. NCBI nr
Match: XP_022927344.1 (caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 592.8 bits (1527), Expect = 1.8e-165
Identity = 286/321 (89.10%), Postives = 301/321 (93.77%), Query Frame = 0


Query: 1   MNEEQEEEENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPP 60
           MNE++ E+ + + FLNYWGNT EE+YFASQRIKASKSFYTSPRG KLFTRSWLPLPP PP
Sbjct: 12  MNEQESEQGHGRSFLNYWGNTSEEDYFASQRIKASKSFYTSPRGFKLFTRSWLPLPPTPP 71

Query: 61  RALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDC 120
           RALIFMVHGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDC
Sbjct: 72  RALIFMVHGYGNNISWTFQATSIFLAQKGFACFALDLEGHGRSQGLKAFVPNVDSVVHDC 131

Query: 121 LCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPR 180
           L FFNF+K DPQFQGLPSFLYGESMG AICLLIHFADP GFDGA+LVAPMC+ISDNVKP 
Sbjct: 132 LSFFNFLKLDPQFQGLPSFLYGESMGAAICLLIHFADPKGFDGAILVAPMCKISDNVKPN 191

Query: 181 WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLR 240
           WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLR
Sbjct: 192 WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLR 251

Query: 241 VTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGE 300
           VTEHLSQRL DV LPFIVLHGNADVVTDP+VSK LYEEAKSE+KTIKIYEGMMHS+L+GE
Sbjct: 252 VTEHLSQRLRDVTLPFIVLHGNADVVTDPNVSKTLYEEAKSEEKTIKIYEGMMHSLLYGE 311

Query: 301 TDENVETVRNDILAWFNGRCR 322
           TDENVE VRNDIL+W N RCR
Sbjct: 312 TDENVEIVRNDILSWLNRRCR 332

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BLAST of Moc03g27500 vs. NCBI nr
Match: XP_038894813.1 (caffeoylshikimate esterase [Benincasa hispida])

HSP 1 Score: 590.5 bits (1521), Expect = 8.8e-165
Identity = 287/317 (90.54%), Postives = 298/317 (94.01%), Query Frame = 0


Query: 5   QEEEENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALI 64
           Q+E+E+ K FLNYWGN PEE+YFASQRIKASKSFYTSPRGLKLFTRSWLPLPP PPRALI
Sbjct: 3   QQEQEHPKFFLNYWGNIPEEDYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALI 62

Query: 65  FMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFF 124
           FMVHGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDCL FF
Sbjct: 63  FMVHGYGNNISWTFQATSIFLAQMGFACFALDLEGHGKSQGLKAFVPNVDSVVHDCLSFF 122

Query: 125 NFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIP 184
           NF+K DPQFQGLPSFLYGESMGGAICLLIHFADP  FDGAVLVAPMC+ISDNVKP WPIP
Sbjct: 123 NFLKLDPQFQGLPSFLYGESMGGAICLLIHFADPKAFDGAVLVAPMCKISDNVKPHWPIP 182

Query: 185 QFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEH 244
           QFLTAVAK  PTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLRVTE+
Sbjct: 183 QFLTAVAKIFPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEY 242

Query: 245 LSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDEN 304
           LSQRL DV LPFIVLHGNADVVTDP+VSK LYEEAKSEDKTIKIYEGMMHSML+GETDEN
Sbjct: 243 LSQRLKDVSLPFIVLHGNADVVTDPNVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDEN 302

Query: 305 VETVRNDILAWFNGRCR 322
           VE VRNDIL+W N RCR
Sbjct: 303 VEIVRNDILSWLNERCR 319

[Close]

BLAST of Moc03g27500 vs. NCBI nr
Match: XP_023520207.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 590.5 bits (1521), Expect = 8.8e-165
Identity = 287/327 (87.77%), Postives = 304/327 (92.97%), Query Frame = 0


Query: 1   MNEEQEEE----ENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLP 60
           MNE++ E+     + + FLNYWG+T EE+YFASQRIKASKSFYTSPRG KLFTRSWLPLP
Sbjct: 12  MNEQESEQGHGHGHGRSFLNYWGSTSEEDYFASQRIKASKSFYTSPRGFKLFTRSWLPLP 71

Query: 61  PAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSV 120
           P PPRALIFMVHGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSV
Sbjct: 72  PTPPRALIFMVHGYGNNISWTFQATSIFLAQKGFACFALDLEGHGRSQGLKAFVPNVDSV 131

Query: 121 VHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDN 180
           VHDCL FFNF+K DPQFQGLPSFLYGESMGGAICLLIHFADP GFDGA+LVAPMC+ISDN
Sbjct: 132 VHDCLSFFNFLKLDPQFQGLPSFLYGESMGGAICLLIHFADPKGFDGAILVAPMCKISDN 191

Query: 181 VKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVL 240
           VKP WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+
Sbjct: 192 VKPNWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVV 251

Query: 241 ELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSM 300
           ELLRVTEHLSQRL DV LPFIVLHGNADVVTDP+VSK LYEEAKSE+KTIKIYEGMMHS+
Sbjct: 252 ELLRVTEHLSQRLRDVTLPFIVLHGNADVVTDPNVSKTLYEEAKSEEKTIKIYEGMMHSL 311

Query: 301 LFGETDENVETVRNDILAWFNGRCRRK 324
           L+GETDENVE VRNDIL+W NGRCR +
Sbjct: 312 LYGETDENVEIVRNDILSWLNGRCRTR 338

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BLAST of Moc03g27500 vs. NCBI nr
Match: XP_008442222.1 (PREDICTED: caffeoylshikimate esterase [Cucumis melo] >KAA0056957.1 caffeoylshikimate esterase [Cucumis melo var. makuwa] >TYK26384.1 caffeoylshikimate esterase [Cucumis melo var. makuwa])

HSP 1 Score: 589.7 bits (1519), Expect = 1.5e-164
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0


Query: 8   EENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMV 67
           +E+ + FLNYWGN PEE+YF SQRIKASKSFYTSPRGLKLFTRSWLPLPP PPRALIFMV
Sbjct: 4   QEHPQSFLNYWGNIPEEDYFTSQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMV 63

Query: 68  HGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFV 127
           HGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDCL FFNF+
Sbjct: 64  HGYGNNISWTFQATSIFLAQMGFACFALDLEGHGRSQGLKAFVPNVDSVVHDCLSFFNFL 123

Query: 128 KQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFL 187
           K DPQFQGLP FLYGESMGGAICL+IHFADP GFDGAVLVAPMC+ISDNVKP WPIPQFL
Sbjct: 124 KLDPQFQGLPCFLYGESMGGAICLMIHFADPKGFDGAVLVAPMCKISDNVKPHWPIPQFL 183

Query: 188 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQ 247
           TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLRVTEHLSQ
Sbjct: 184 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEHLSQ 243

Query: 248 RLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVET 307
           RL DV LPFIVLHGNADVVTDP VSK LYEEAKSEDKTIKIYEGMMHSML+GETDENVE 
Sbjct: 244 RLKDVSLPFIVLHGNADVVTDPDVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDENVEI 303

Query: 308 VRNDILAWFNGRCR 322
           VRNDIL W N RCR
Sbjct: 304 VRNDILRWLNERCR 317

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BLAST of Moc03g27500 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 5.3e-64
Identity = 131/328 (39.94%), Postives = 195/328 (59.45%), Query Frame = 0


Query: 1   MNEEQEEEENRKPFL-----NYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPL 60
           M  E E   N  P       N+WG  PEEEY+ SQ ++ SKS++ +P G KLFT+S+LPL
Sbjct: 1   MPSEAESSANSAPATPPPPPNFWGTMPEEEYYTSQGVRNSKSYFETPNG-KLFTQSFLPL 60

Query: 61  PPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDS 120
                +  ++M HGYG++ SW FQ   +  +  G+A FA DL GHG S G++ ++ +++ 
Sbjct: 61  -DGEIKGTVYMSHGYGSDTSWMFQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEK 120

Query: 121 VVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHF-ADPLGFDGAVLVAPMCRIS 180
           V    L FF  V+    ++ LP+FL+GESMGG + LL++F ++P  + G +  AP+  I 
Sbjct: 121 VAATSLAFFKHVRCSDPYKDLPAFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIP 180

Query: 181 DNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGT 240
           +++KP          +     T A +P   ++ K++K  EK  +   NP RY GKPR+GT
Sbjct: 181 EDMKPSKAHLFAYGLLFGLADTWAAMPDNKMVGKAIKDPEKLKIIASNPQRYTGKPRVGT 240

Query: 241 VLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMH 300
           + ELLR T+++ +    V +P    HG AD VT P+ SK LYE+A S DKT+KIYEGM H
Sbjct: 241 MRELLRKTQYVQENFGKVTIPVFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYH 300

Query: 301 SMLFGETDENVETVRNDILAWFNGRCRR 323
           S++ GE DEN E V  D+  W + + ++
Sbjct: 301 SLIQGEPDENAEIVLKDMREWIDEKVKK 326

[Close]

BLAST of Moc03g27500 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 7.5e-26
Identity = 93/280 (33.21%), Postives = 139/280 (49.64%), Query Frame = 0


Query: 44  GLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGAS 103
           G  LF R W   P   P+ALIF+ HG G + S  ++  +  L       FA D  GHG S
Sbjct: 27  GQYLFCRYW--KPTGTPKALIFVSHGAGEH-SGRYEELARMLMGLDLLVFAHDHVGHGQS 86

Query: 104 QGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDG 163
           +G +  V +    V D L   + +++D  + GLP FL G SMGGAI +L     P  F G
Sbjct: 87  EGERMVVSDFHVFVRDVLQHVDSMQKD--YPGLPVFLLGHSMGGAIAILTAAERPGHFAG 146

Query: 164 AVLVAPMCRISDNVKPRWPI--PQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEM 223
            VL++P+   +      + +   + L  V   LP L++ P    +D SV    K  V   
Sbjct: 147 MVLISPLVLANPESATTFKVLAAKVLNLV---LPNLSLGP----IDSSVLSRNKTEVDIY 206

Query: 224 N--PMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEA 283
           N  P+  R   ++   ++LL     + + L  + +PF++L G+AD + D   +  L E A
Sbjct: 207 NSDPLICRAGLKVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAYLLMELA 266

Query: 284 KSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFNGR 320
           KS+DKT+KIYEG  H +L  E  E   +V ++I  W + R
Sbjct: 267 KSQDKTLKIYEGAYH-VLHKELPEVTNSVFHEINMWVSQR 293

[Close]

BLAST of Moc03g27500 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.4e-24
Identity = 84/278 (30.22%), Postives = 133/278 (47.84%), Query Frame = 0


Query: 44  GLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGAS 103
           G  LF R W   P   P+ALIF+ HG G +    +   +  L       FA D  GHG S
Sbjct: 27  GQYLFCRYW--KPSGTPKALIFVSHGAGEHCG-RYDELAHMLKGLDMLVFAHDHVGHGQS 86

Query: 104 QGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDG 163
           +G +  V +    V D L   + +++D  +  +P FL G SMGGAI +L+    P  F G
Sbjct: 87  EGERMVVSDFQVFVRDVLQHVDTIQKD--YPDVPIFLLGHSMGGAISILVAAERPTYFSG 146

Query: 164 AVLVAPMCRISDNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMN- 223
            VL++P+   +        +       AK L  +    T   +D SV    K  V   N 
Sbjct: 147 MVLISPLVLANPESASTLKV-----LAAKLLNFVLPNMTLGRIDSSVLSRNKSEVDLYNS 206

Query: 224 -PMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKS 283
            P+  R   ++   ++LL     + + +  + LPF++L G+AD + D   +  L E ++S
Sbjct: 207 DPLVCRAGLKVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSRS 266

Query: 284 EDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFNGR 320
           +DKT+K+YEG  H +L  E  E   +V +++ +W + R
Sbjct: 267 QDKTLKMYEGAYH-VLHRELPEVTNSVLHEVNSWVSHR 293

[Close]

BLAST of Moc03g27500 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 4.1e-24
Identity = 86/279 (30.82%), Postives = 134/279 (48.03%), Query Frame = 0


Query: 44  GLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGAS 103
           G  LF R W   P   P+ALIF+ HG G +    +   +  L +     FA D  GHG S
Sbjct: 27  GQYLFCRYW--KPSGTPKALIFVSHGAGEHCG-RYDELAQMLKRLDMLVFAHDHVGHGQS 86

Query: 104 QGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDG 163
           +G +  V +    V D L   N V++D  +  +P FL G SMGGAI +L     P  F G
Sbjct: 87  EGERMVVSDFQVFVRDLLQHVNTVQKD--YPEVPVFLLGHSMGGAISILAAAERPTHFSG 146

Query: 164 AVLVAPMCRISDNVKPRWPIPQFLTAVAKF-LPTLAIVPTADLLDKSVKVEEKKIVAEMN 223
            +L++P+  I  N +    +      +  F LP +++      +D SV    K  V   N
Sbjct: 147 MILISPL--ILANPESASTLKVLAAKLLNFVLPNISL----GRIDSSVLSRNKSEVDLYN 206

Query: 224 --PMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAK 283
             P+      ++   ++LL     + + +  + LPF++L G+AD + D   +  L E + 
Sbjct: 207 SDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDSKGAYLLMESSP 266

Query: 284 SEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFNGR 320
           S+DKT+K+YEG  H +L  E  E   +V ++I  W + R
Sbjct: 267 SQDKTLKMYEGAYH-VLHKELPEVTNSVLHEINTWVSHR 293

[Close]

BLAST of Moc03g27500 vs. ExPASy Swiss-Prot
Match: P28321 (Monoglyceride lipase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YJU3 PE=1 SV=2)

HSP 1 Score: 87.0 bits (214), Expect = 4.1e-16
Identity = 71/278 (25.54%), Postives = 115/278 (41.37%), Query Frame = 0


Query: 61  RALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHG-ASQGLKAFVPNVDSVVHD 120
           R  + ++HG+G      F+     L+ +G+  F  D  G G  S G    V +   V +D
Sbjct: 40  RGRVLLIHGFGEYTKIQFRLMD-HLSLNGYESFTFDQRGAGVTSPGRSKGVTDEYHVFND 99

Query: 121 CLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLI-----HFADPLGFDGAVLVAPMCRIS 180
              F      + + +G+P F++G SMGG ICL       H  +  G+ G+    P+  + 
Sbjct: 100 LEHFVEKNLSECKAKGIPLFMWGHSMGGGICLNYACQGKHKNEISGYIGS---GPLIILH 159

Query: 181 DNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGT 240
            +     P       +AKFLP + I    DL         K I ++     + G   +  
Sbjct: 160 PHTMYNKPTQIIAPLLAKFLPRVRIDTGLDL---------KGITSDKAYRAFLGSDPMSV 219

Query: 241 VL---------------ELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEA 300
            L               +L +   +  Q+      P I++HG  D + DP  S+   ++ 
Sbjct: 220 PLYGSFRQIHDFMQRGAKLYKNENNYIQKNFAKDKPVIIMHGQDDTINDPKGSEKFIQDC 279

Query: 301 KSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFN 318
            S DK +K+Y G  HS+   ETD+   TV ND+  W +
Sbjct: 280 PSADKELKLYPGARHSIFSLETDKVFNTVFNDMKQWLD 304

[Close]

BLAST of Moc03g27500 vs. ExPASy TrEMBL
Match: A0A6J1KJF3 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111495780 PE=4 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 6.6e-166
Identity = 288/323 (89.16%), Postives = 303/323 (93.81%), Query Frame = 0


Query: 1   MNEEQEEE--ENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPA 60
           MNE++ E+   + + FLNYWGNT EE+YFASQRIKASKSFYTSPRG KLFTRSWLPLPP 
Sbjct: 12  MNEQESEQGHGHGRSFLNYWGNTSEEDYFASQRIKASKSFYTSPRGFKLFTRSWLPLPPT 71

Query: 61  PPRALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVH 120
           PPRALIFMVHGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVH
Sbjct: 72  PPRALIFMVHGYGNNISWTFQATSIFLAQKGFACFALDLEGHGRSQGLKAFVPNVDSVVH 131

Query: 121 DCLCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVK 180
           DCL FFNF+K DPQFQGLPSFLYGESMGGAICLLIHFADP GFDGA+LVAPMC+ISDNVK
Sbjct: 132 DCLSFFNFLKLDPQFQGLPSFLYGESMGGAICLLIHFADPKGFDGAILVAPMCKISDNVK 191

Query: 181 PRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLEL 240
           P WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+EL
Sbjct: 192 PNWPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVEL 251

Query: 241 LRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLF 300
           LRVTEHLSQRL DV LPFIVLHGNADVVTDP+VSK LYEEAKSEDKTIKIYEGMMHS+L+
Sbjct: 252 LRVTEHLSQRLRDVTLPFIVLHGNADVVTDPNVSKTLYEEAKSEDKTIKIYEGMMHSLLY 311

Query: 301 GETDENVETVRNDILAWFNGRCR 322
           GE+DENVE VRNDIL+W NGRCR
Sbjct: 312 GESDENVEIVRNDILSWLNGRCR 334

[Close]

BLAST of Moc03g27500 vs. ExPASy TrEMBL
Match: A0A6J1EHR6 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111434200 PE=4 SV=1)

HSP 1 Score: 592.8 bits (1527), Expect = 8.6e-166
Identity = 286/321 (89.10%), Postives = 301/321 (93.77%), Query Frame = 0


Query: 1   MNEEQEEEENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPP 60
           MNE++ E+ + + FLNYWGNT EE+YFASQRIKASKSFYTSPRG KLFTRSWLPLPP PP
Sbjct: 12  MNEQESEQGHGRSFLNYWGNTSEEDYFASQRIKASKSFYTSPRGFKLFTRSWLPLPPTPP 71

Query: 61  RALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDC 120
           RALIFMVHGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDC
Sbjct: 72  RALIFMVHGYGNNISWTFQATSIFLAQKGFACFALDLEGHGRSQGLKAFVPNVDSVVHDC 131

Query: 121 LCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPR 180
           L FFNF+K DPQFQGLPSFLYGESMG AICLLIHFADP GFDGA+LVAPMC+ISDNVKP 
Sbjct: 132 LSFFNFLKLDPQFQGLPSFLYGESMGAAICLLIHFADPKGFDGAILVAPMCKISDNVKPN 191

Query: 181 WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLR 240
           WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLR
Sbjct: 192 WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLR 251

Query: 241 VTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGE 300
           VTEHLSQRL DV LPFIVLHGNADVVTDP+VSK LYEEAKSE+KTIKIYEGMMHS+L+GE
Sbjct: 252 VTEHLSQRLRDVTLPFIVLHGNADVVTDPNVSKTLYEEAKSEEKTIKIYEGMMHSLLYGE 311

Query: 301 TDENVETVRNDILAWFNGRCR 322
           TDENVE VRNDIL+W N RCR
Sbjct: 312 TDENVEIVRNDILSWLNRRCR 332

[Close]

BLAST of Moc03g27500 vs. ExPASy TrEMBL
Match: A0A5D3DS62 (Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00460 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.3e-165
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0


Query: 8   EENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMV 67
           +E+ + FLNYWGN PEE+YF SQRIKASKSFYTSPRGLKLFTRSWLPLPP PPRALIFMV
Sbjct: 4   QEHPQSFLNYWGNIPEEDYFTSQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMV 63

Query: 68  HGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFV 127
           HGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDCL FFNF+
Sbjct: 64  HGYGNNISWTFQATSIFLAQMGFACFALDLEGHGRSQGLKAFVPNVDSVVHDCLSFFNFL 123

Query: 128 KQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFL 187
           K DPQFQGLP FLYGESMGGAICL+IHFADP GFDGAVLVAPMC+ISDNVKP WPIPQFL
Sbjct: 124 KLDPQFQGLPCFLYGESMGGAICLMIHFADPKGFDGAVLVAPMCKISDNVKPHWPIPQFL 183

Query: 188 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQ 247
           TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLRVTEHLSQ
Sbjct: 184 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEHLSQ 243

Query: 248 RLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVET 307
           RL DV LPFIVLHGNADVVTDP VSK LYEEAKSEDKTIKIYEGMMHSML+GETDENVE 
Sbjct: 244 RLKDVSLPFIVLHGNADVVTDPDVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDENVEI 303

Query: 308 VRNDILAWFNGRCR 322
           VRNDIL W N RCR
Sbjct: 304 VRNDILRWLNERCR 317

[Close]

BLAST of Moc03g27500 vs. ExPASy TrEMBL
Match: A0A1S3B5V5 (caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103486137 PE=4 SV=1)

HSP 1 Score: 589.7 bits (1519), Expect = 7.3e-165
Identity = 285/314 (90.76%), Postives = 294/314 (93.63%), Query Frame = 0


Query: 8   EENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMV 67
           +E+ + FLNYWGN PEE+YF SQRIKASKSFYTSPRGLKLFTRSWLPLPP PPRALIFMV
Sbjct: 4   QEHPQSFLNYWGNIPEEDYFTSQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMV 63

Query: 68  HGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFV 127
           HGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDCL FFNF+
Sbjct: 64  HGYGNNISWTFQATSIFLAQMGFACFALDLEGHGRSQGLKAFVPNVDSVVHDCLSFFNFL 123

Query: 128 KQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFL 187
           K DPQFQGLP FLYGESMGGAICL+IHFADP GFDGAVLVAPMC+ISDNVKP WPIPQFL
Sbjct: 124 KLDPQFQGLPCFLYGESMGGAICLMIHFADPKGFDGAVLVAPMCKISDNVKPHWPIPQFL 183

Query: 188 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQ 247
           TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLRVTEHLSQ
Sbjct: 184 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEHLSQ 243

Query: 248 RLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVET 307
           RL DV LPFIVLHGNADVVTDP VSK LYEEAKSEDKTIKIYEGMMHSML+GETDENVE 
Sbjct: 244 RLKDVSLPFIVLHGNADVVTDPDVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDENVEI 303

Query: 308 VRNDILAWFNGRCR 322
           VRNDIL W N RCR
Sbjct: 304 VRNDILRWLNERCR 317

[Close]

BLAST of Moc03g27500 vs. ExPASy TrEMBL
Match: A0A0A0LUH8 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073040 PE=4 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.0e-164
Identity = 283/314 (90.13%), Postives = 294/314 (93.63%), Query Frame = 0


Query: 8   EENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMV 67
           +E+ + FLNYWGN  EE+YF SQRIKASKSFYTSPRGLKLFTRSWLPLPP PPRALIFMV
Sbjct: 4   QEHPQSFLNYWGNISEEDYFTSQRIKASKSFYTSPRGLKLFTRSWLPLPPTPPRALIFMV 63

Query: 68  HGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFV 127
           HGYGNNISWTFQATSIFLAQ GFACFALDLEGHG SQGLKAFVPNVDSVVHDCL FFNF+
Sbjct: 64  HGYGNNISWTFQATSIFLAQMGFACFALDLEGHGRSQGLKAFVPNVDSVVHDCLSFFNFL 123

Query: 128 KQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFL 187
           K DPQFQGLP FLYGESMGGAICL+IHFADP GFDGAVLVAPMC+ISDNVKP WPIPQFL
Sbjct: 124 KLDPQFQGLPCFLYGESMGGAICLMIHFADPKGFDGAVLVAPMCKISDNVKPHWPIPQFL 183

Query: 188 TAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQ 247
           TAVA+FLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTV+ELLRVTEHLSQ
Sbjct: 184 TAVAQFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVVELLRVTEHLSQ 243

Query: 248 RLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVET 307
           RL DV LPFIVLHGNADVVTDP+VSK LYEEAKSEDKTIKIYEGMMHSML+GETDENVE 
Sbjct: 244 RLKDVNLPFIVLHGNADVVTDPNVSKTLYEEAKSEDKTIKIYEGMMHSMLYGETDENVEI 303

Query: 308 VRNDILAWFNGRCR 322
           VRNDIL W N RCR
Sbjct: 304 VRNDILCWLNERCR 317

[Close]

BLAST of Moc03g27500 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 468.4 bits (1204), Expect = 4.7e-132
Identity = 218/320 (68.12%), Postives = 268/320 (83.75%), Query Frame = 0


Query: 1   MNEEQEEEENRKPFLNYWGNTPEEEYFASQRIKASKSFYTSPRGLKLFTRSWLPLPPAPP 60
           M+++Q E E     +N+WG T EE+YF  + I  SKSF+TSPRGL LFTRSWLP   +PP
Sbjct: 1   MSDQQLETE-----INFWGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPP 60

Query: 61  RALIFMVHGYGNNISWTFQATSIFLAQSGFACFALDLEGHGASQGLKAFVPNVDSVVHDC 120
           R LIFMVHGYGN++SWTFQ+T IFLAQ GFACFALD+EGHG S G++A+VP+VD VV D 
Sbjct: 61  RGLIFMVHGYGNDVSWTFQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDI 120

Query: 121 LCFFNFVKQDPQFQGLPSFLYGESMGGAICLLIHFADPLGFDGAVLVAPMCRISDNVKPR 180
           + FFN +KQ+P+FQGLP FL+GESMGGAICLLI FADPLGFDGAVLVAPMC+ISD V+P+
Sbjct: 121 ISFFNSIKQNPKFQGLPRFLFGESMGGAICLLIQFADPLGFDGAVLVAPMCKISDKVRPK 180

Query: 181 WPIPQFLTAVAKFLPTLAIVPTADLLDKSVKVEEKKIVAEMNPMRYRGKPRLGTVLELLR 240
           WP+ QFL  +++FLPT AIVPT DLL+KS+KVEEKK +A+ NPMRY  KPRLGTV+ELLR
Sbjct: 181 WPVDQFLIMISRFLPTWAIVPTEDLLEKSIKVEEKKPIAKRNPMRYNEKPRLGTVMELLR 240

Query: 241 VTEHLSQRLSDVRLPFIVLHGNADVVTDPSVSKALYEEAKSEDKTIKIYEGMMHSMLFGE 300
           VT++L ++L DV +PFI++HG+AD VTDP VS+ LYE AKS+DKT+KIY+GMMHSMLFGE
Sbjct: 241 VTDYLGKKLKDVSIPFIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGE 300

Query: 301 TDENVETVRNDILAWFNGRC 321
            D+N+E VR DI++W N RC
Sbjct: 301 PDDNIEIVRKDIVSWLNDRC 315

[Close]

BLAST of Moc03g27500 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 261.9 bits (668), Expect = 6.7e-70
Identity = 131/289 (45.33%), Postives = 185/289 (64.01%), Query Frame = 0


Query: 29  SQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQS 88
           ++ IK  +SF  + RG+KLFT  W+P     P+AL+F+ HGY    S T  +T+  L ++
Sbjct: 5   TENIKYEESFIKNTRGMKLFTCKWVP-AKQEPKALVFICHGYAMECSITMNSTARRLVKA 64

Query: 89  GFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGA 148
           GFA + +D EGHG S GL A+VPN D +V D    +  + +  + +G   FL GESMGGA
Sbjct: 65  GFAVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGA 124

Query: 149 ICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDK 208
           + LL+H   P  +DGAVLVAPMC+I++ +KP   +   L  ++  +P+  I+P  D+++ 
Sbjct: 125 VLLLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIET 184

Query: 209 SVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTD 268
           + K  E +     NP  Y+G+PRL T  ELLRV+  L +RL++V LPFIVLHG  D VTD
Sbjct: 185 AFKQPEIRKQVRENPYCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTD 244

Query: 269 PSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFN 318
            +VS+ LYE A S DKT K+Y GM H +L+GET EN+ETV  DI+ W +
Sbjct: 245 KAVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWLD 292

[Close]

BLAST of Moc03g27500 vs. TAIR 10
Match: AT5G16120.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.4e-69
Identity = 125/286 (43.71%), Postives = 187/286 (65.38%), Query Frame = 0


Query: 32  IKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFA 91
           IK  +SF  + RG+++F++SWLP   + PRAL+   HGYG+  ++ F+  +  LA SG+ 
Sbjct: 53  IKTKESFEVNSRGVEIFSKSWLP-EASKPRALVCFCHGYGDTCTFFFEGIARRLALSGYG 112

Query: 92  CFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICL 151
            FA+D  G G S+GL  ++P+ D +V D +  ++ +K +P+F  LPSFL+G+SMGGA+ L
Sbjct: 113 VFAMDYPGFGLSEGLHGYIPSFDLLVQDVIEHYSNIKANPEFSSLPSFLFGQSMGGAVSL 172

Query: 152 LIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVK 211
            IH   P  + GAVL+APMC+I+D++ P   + Q L  +A  LP   +VP  DL +   +
Sbjct: 173 KIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPKHKLVPQKDLAEAGFR 232

Query: 212 VEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSV 271
              K+ +   N + Y GKPRL T +E+LR T+ + ++L +V LP ++LHG AD VTDPSV
Sbjct: 233 DIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQEVSLPILILHGEADTVTDPSV 292

Query: 272 SKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFN 318
           S+ LYE+AKS DK I +YE   HS+L GE D+ +  V +DI++W N
Sbjct: 293 SRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMILRVLSDIISWLN 337

[Close]

BLAST of Moc03g27500 vs. TAIR 10
Match: AT5G16120.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 7.4e-69
Identity = 125/286 (43.71%), Postives = 187/286 (65.38%), Query Frame = 0


Query: 32  IKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQSGFA 91
           IK  +SF  + RG+++F++SWLP   + PRAL+   HGYG+  ++ F+  +  LA SG+ 
Sbjct: 71  IKTKESFEVNSRGVEIFSKSWLP-EASKPRALVCFCHGYGDTCTFFFEGIARRLALSGYG 130

Query: 92  CFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGAICL 151
            FA+D  G G S+GL  ++P+ D +V D +  ++ +K +P+F  LPSFL+G+SMGGA+ L
Sbjct: 131 VFAMDYPGFGLSEGLHGYIPSFDLLVQDVIEHYSNIKANPEFSSLPSFLFGQSMGGAVSL 190

Query: 152 LIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDKSVK 211
            IH   P  + GAVL+APMC+I+D++ P   + Q L  +A  LP   +VP  DL +   +
Sbjct: 191 KIHLKQPNAWAGAVLLAPMCKIADDLVPPPVLKQILIGLANVLPKHKLVPQKDLAEAGFR 250

Query: 212 VEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTDPSV 271
              K+ +   N + Y GKPRL T +E+LR T+ + ++L +V LP ++LHG AD VTDPSV
Sbjct: 251 DIRKRDMTPYNMICYSGKPRLRTAVEMLRTTQDIEKQLQEVSLPILILHGEADTVTDPSV 310

Query: 272 SKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFN 318
           S+ LYE+AKS DK I +YE   HS+L GE D+ +  V +DI++W N
Sbjct: 311 SRELYEKAKSPDKKIVLYENAYHSLLEGEPDDMILRVLSDIISWLN 355

[Close]

BLAST of Moc03g27500 vs. TAIR 10
Match: AT2G47630.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 257.3 bits (656), Expect = 1.6e-68
Identity = 125/291 (42.96%), Postives = 182/291 (62.54%), Query Frame = 0


Query: 29  SQRIKASKSFYTSPRGLKLFTRSWLPLPPAPPRALIFMVHGYGNNISWTFQATSIFLAQS 88
           S   +  + +  + RG++LF   W+P   + P+AL+F+ HGYG   S + +   I LA +
Sbjct: 3   SSEFQYHEEYVRNSRGVELFACRWIPSSSSSPKALVFLCHGYGMECSDSMKECGIRLASA 62

Query: 89  GFACFALDLEGHGASQGLKAFVPNVDSVVHDCLCFFNFVKQDPQFQGLPSFLYGESMGGA 148
           G+A F +D EGHG S G + ++    +VV+DC  ++  +    ++     FLYGESMGGA
Sbjct: 63  GYAVFGMDYEGHGRSMGSRCYIKKFANVVNDCYDYYTSICAQEEYMDKGRFLYGESMGGA 122

Query: 149 ICLLIHFADPLGFDGAVLVAPMCRISDNVKPRWPIPQFLTAVAKFLPTLAIVPTADLLDK 208
           + LL+H  DPL ++GA+LVAPMC+IS+ VKP   +   LT V + +P   IVPT D++D 
Sbjct: 123 VTLLLHKKDPLFWNGAILVAPMCKISEKVKPHPIVINLLTRVEEIIPKWKIVPTKDVIDA 182

Query: 209 SVKVEEKKIVAEMNPMRYRGKPRLGTVLELLRVTEHLSQRLSDVRLPFIVLHGNADVVTD 268
           + K   K+     N + Y+ KPRL T LE+LR + +L   L ++ +PF VLHG AD VTD
Sbjct: 183 AFKDLVKREEVRNNKLIYQDKPRLKTALEMLRTSMNLEDTLHEITMPFFVLHGEADTVTD 242

Query: 269 PSVSKALYEEAKSEDKTIKIYEGMMHSMLFGETDENVETVRNDILAWFNGR 320
           P VSKALYE+A + DKT+K+Y GM H++  GE D NV+ V  DI+ W + R
Sbjct: 243 PEVSKALYEKASTRDKTLKLYPGMWHALTSGEPDCNVDLVFADIINWLDLR 293

The following BLAST results are available for this feature:

BLAST of Moc03g27500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023001731.1	1.4e-165	89.16	caffeoylshikimate esterase-like [Cucurbita maxima]	[more]
XP_022927344.1	1.8e-165	89.10	caffeoylshikimate esterase-like [Cucurbita moschata]	[more]
XP_038894813.1	8.8e-165	90.54	caffeoylshikimate esterase [Benincasa hispida]	[more]
XP_023520207.1	8.8e-165	87.77	caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_008442222.1	1.5e-164	90.76	PREDICTED: caffeoylshikimate esterase [Cucumis melo] >KAA0056957.1 caffeoylshiki...	[more]

BLAST of Moc03g27500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	5.3e-64	39.94	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
Q99685	7.5e-26	33.21	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]
O35678	2.4e-24	30.22	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q8R431	4.1e-24	30.82	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]
P28321	4.1e-16	25.54	Monoglyceride lipase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX...	[more]

BLAST of Moc03g27500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KJF3	6.6e-166	89.16	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111495780 PE=4...	[more]
A0A6J1EHR6	8.6e-166	89.10	caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111434200 PE...	[more]
A0A5D3DS62	7.3e-165	90.76	Caffeoylshikimate esterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3B5V5	7.3e-165	90.76	caffeoylshikimate esterase OS=Cucumis melo OX=3656 GN=LOC103486137 PE=4 SV=1	[more]
A0A0A0LUH8	8.0e-164	90.13	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G073040...	[more]

BLAST of Moc03g27500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G11090.1	4.7e-132	68.13	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39420.1	6.7e-70	45.33	alpha/beta-Hydrolases superfamily protein	[more]
AT5G16120.1	7.4e-69	43.71	alpha/beta-Hydrolases superfamily protein	[more]
AT5G16120.2	7.4e-69	43.71	alpha/beta-Hydrolases superfamily protein	[more]
AT2G47630.1	1.6e-68	42.96	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 59..300 e-value: 1.9E-54 score: 184.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 25..321 e-value: 4.3E-55 score: 189.0
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 26..319
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 19..319
None	No IPR available	PANTHER	PTHR11614:SF20	ALPHA/BETA-HYDROLASES SUPERFAMILY PROTEIN	coord: 19..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g27500.1	Moc03g27500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity