Moc03g27430 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g27430 vs. NCBI nr
Match: ALJ53304.1 (polyphenol oxidase [Luffa aegyptiaca] >APO14263.1 polyphenol oxidase 4 [Luffa aegyptiaca])

HSP 1 Score: 599.4 bits (1544), Expect = 3.5e-167
Identity = 329/552 (59.60%), Postives = 387/552 (70.11%), Query Frame = 0

Query: 56  NNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDT 115
           +NG +TP + SNP E G S++KFDRRDVLFGLGGLY  A+L G DR ALAD    ISPD 
Sbjct: 38  SNGVQTP-MNSNPQE-GDSMSKFDRRDVLFGLGGLYSAAAL-GRDRSALAD---PISPDV 97

Query: 116 ANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLM 175
            +C P    A  + +CCPP I DI DF+ PP + + RVRPAAHLIT EQLAK E+A+  M
Sbjct: 98  IHCEPITLEADEKVDCCPPSILDITDFV-PPAVENLRVRPAAHLITQEQLAKLEEAVTKM 157

Query: 176 KHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERIL 235
           K L  DDPR F  QA+VHCAYCN AYKQ D  P+ + ++HWN LFFPFHRCYLYF ERIL
Sbjct: 158 KALDPDDPRNFYQQARVHCAYCNDAYKQ-DGFPELDFDIHWNWLFFPFHRCYLYFHERIL 217

Query: 236 GYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPD--SSLYDRLRDARHVPPKLAYLNYDG-D 295
           G LI+DP FAIPFWNWD+ PGM+MP IY N D  S LYD LRD  H PPKL  LNYDG  
Sbjct: 218 GSLINDPDFAIPFWNWDAIPGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDGAT 277

Query: 296 EDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWV 355
           EDP +DV+ CN R M +QM +  K+A GF G+ +R G+EA   GMGSIE S HNN+H W 
Sbjct: 278 EDPSYDVLFCNPRLMYRQMVINSKTAEGFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWS 337

Query: 356 GDPNSPHNEDMGTFYSAG---MFFDSSNECMKLET--------KRRDYTDPDWLDSSFVL 415
           GDP   + EDMG FY+A    +F+       ++ T        K RD+TDPDWL++SFV 
Sbjct: 338 GDPQQKYREDMGHFYAAARDPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVF 397

Query: 416 YDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVY 475
           YDENKNA+RIKV+DC+D+  LGY YQ V+IPWL+T+ IPRQ      S +    PP EV 
Sbjct: 398 YDENKNAIRIKVQDCLDNEKLGYKYQAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV- 457

Query: 476 FPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDV 535
               LNS++   V RP RSR+  QK  EDEVLVIE +E D +SRVHFNVLVNVLD+G DV
Sbjct: 458 ----LNSKMTKEVKRPQRSRSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDV 517

Query: 536 SPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVT 593
           SP N EFAGSF++IP  H H  A  SL  VITELIEELG DD ET+ V I PKLGG+ VT
Sbjct: 518 SPGNTEFAGSFKSIPLGHKH-RANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVT 574

BLAST of Moc03g27430 vs. NCBI nr
Match: XP_023542623.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 591.3 bits (1523), Expect = 9.4e-165
Identity = 304/560 (54.29%), Postives = 386/560 (68.93%), Query Frame = 0

Query: 45  IRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFA 104
           I+    KL + + G    P   SN    G S +KFDRRDVLFGLGGLYG A+L+     A
Sbjct: 13  IKNQTSKLQNKSRGISHVPCRASN----GESTSKFDRRDVLFGLGGLYGAAALTNQQSPA 72

Query: 105 LADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPE 164
            A     ++PD  NC PA         CCPPKIH IIDFI PP     R+RP AH +TP+
Sbjct: 73  SA---APLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRPPAHSLTPK 132

Query: 165 QLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPF 224
           Q   FEKAI++MK LP +DPR F  QA VHCAYC+YAYKQL   P AE++VHWNALFFPF
Sbjct: 133 QQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFPF 192

Query: 225 HRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPK 284
           HR YLYFFERILG+LI++P F IPFWNWD+  GM+MPKIY+NP+S LYD+LR+ RH P K
Sbjct: 193 HRAYLYFFERILGHLINEPNFVIPFWNWDNNEGMQMPKIYDNPNSPLYDKLRNPRHRPHK 252

Query: 285 LAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIES 344
           L  LNY GD++P +DVVDCNLRWM+KQM     +A GF G+L+    EA +  GMG+IE 
Sbjct: 253 LLNLNYHGDDNPSYDVVDCNLRWMHKQMITDADTAEGFHGKLLIASHEANEETGMGTIER 312

Query: 345 SPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYTD 404
           SPHNN+H+WVGDPN+ ++ +MG FYSA    +F+       ++          K RD+ +
Sbjct: 313 SPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFDN 372

Query: 405 PDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPE 464
           PDWL+SSFV Y+E+K AVRIKV DC+D+  LGYVYQ VD+PWL+T+Q PR      ++  
Sbjct: 373 PDWLNSSFVFYNEHKQAVRIKVSDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKATQA 432

Query: 465 PPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVL 524
           P +     + FP  LNS + T V RP++ R  ++K E+DEVLVIE IE D+   VHFNVL
Sbjct: 433 PQV-----IKFPQGLNSMVTTTVKRPNKLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVL 492

Query: 525 VNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKI 584
           VNV+D+G DV P N EF G+F  +PH HNH   K SLR VITE+++ELG++D E++ V++
Sbjct: 493 VNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVRL 552

Query: 585 APKLGGENVTIGGIKIELQP 591
            PK+GG+ VTIGG+KIELQP
Sbjct: 553 DPKVGGDKVTIGGMKIELQP 558

BLAST of Moc03g27430 vs. NCBI nr
Match: XP_022994369.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-163
Identity = 298/533 (55.91%), Postives = 379/533 (71.11%), Query Frame = 0

Query: 72  GISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRN-- 131
           G S +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC PA T  G  +  
Sbjct: 40  GDSTSKFDRRDVLFGLGGLYGAAALTNQQSPASA---KPLAPDVNNCEPA-TIVGNEHIP 99

Query: 132 CCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQA 191
           CCPPKIHDIIDFI PP     R+R  AH +TP+Q   FEKAI++MK L  +DPR F  QA
Sbjct: 100 CCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQA 159

Query: 192 QVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWN 251
            VHCAYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P FAIPFWN
Sbjct: 160 DVHCAYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWN 219

Query: 252 WDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQ 311
           WD+  GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++PP+DVVDCNLRWM+KQ
Sbjct: 220 WDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQ 279

Query: 312 MKVAGKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSA 371
           M     +A GF G+L+   +EA +  GMG+IE SPHNN+H+WVGDPN+ +N +MG FYSA
Sbjct: 280 MITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSA 339

Query: 372 G---MFFDSSNECMKL--------ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVD 431
               +F+       ++          K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D
Sbjct: 340 ARDPLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLD 399

Query: 432 SVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPS 491
           +  LGYVYQ VD+PWL+T+Q PR      ++  P +     + FP  LNS + T V RP 
Sbjct: 400 TRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPK 459

Query: 492 RSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHA 551
           + R  ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  + H 
Sbjct: 460 KLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHG 519

Query: 552 HNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 591
           HNH   K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 520 HNH-RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Moc03g27430 vs. NCBI nr
Match: KAG6573251.1 (hypothetical protein SDJN03_27138, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 587.0 bits (1512), Expect = 1.8e-163
Identity = 301/561 (53.65%), Postives = 388/561 (69.16%), Query Frame = 0

Query: 44  EIRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
           +I+     L + + G +  P   SN D    S +KFDRRDVLFGLGGLYG A+L+     
Sbjct: 15  QIKNQTSNLQNKSRGINHVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTNQQSP 74

Query: 104 ALADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITP 163
           A A     ++PD  NC PA         CCPPKIH IIDFI PP     R+RP AH +TP
Sbjct: 75  ASA---APLAPDVNNCEPATIEGNQHIPCCPPKIHHIIDFIHPPHTTKLRIRPPAHSLTP 134

Query: 164 EQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFP 223
           +Q   FEKAI++MK LP +DPR F  QA VHCAYC+YAYKQL   P AE++VHWNALFFP
Sbjct: 135 KQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFP 194

Query: 224 FHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPP 283
           FHR YLYFFERILG+LI++P F IPFWNWD+  GM+MP+IY++P+S LYD+LR+ RH P 
Sbjct: 195 FHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPH 254

Query: 284 KLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIE 343
           KL  LNY GD++P +DVVD NLRWM+KQM     +A GFLG+L+   +EA +  GMG+IE
Sbjct: 255 KLLNLNYHGDDNPSYDVVDSNLRWMHKQMIKDADTAEGFLGKLLVASDEANEETGMGTIE 314

Query: 344 SSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYT 403
            SPHNN+H+WVGDPN+ ++ +MG FYSA    +F+       ++          K RD+ 
Sbjct: 315 RSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKPRDFD 374

Query: 404 DPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSP 463
           +PDWL+SSFV Y+E+K AVRIKVRDC+D+  LGYVYQ VD+PWL+T+Q PR      ++ 
Sbjct: 375 NPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQ 434

Query: 464 EPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNV 523
            P +     + FP  LNS + T V RP++    ++K E+DEVLVIE IE D+   VHFNV
Sbjct: 435 APQV-----IKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNV 494

Query: 524 LVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVK 583
           LVNV+D+G DV P N EF G+F  +PH HNH   K SLR VITE+++ELG++D E++ V+
Sbjct: 495 LVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVR 554

Query: 584 IAPKLGGENVTIGGIKIELQP 591
           + PK+GG+ VTIGG+KIELQP
Sbjct: 555 LDPKVGGDKVTIGGMKIELQP 561

BLAST of Moc03g27430 vs. NCBI nr
Match: XP_022954613.1 (polyphenol oxidase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 585.9 bits (1509), Expect = 4.0e-163
Identity = 300/561 (53.48%), Postives = 387/561 (68.98%), Query Frame = 0

Query: 44  EIRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
           +I+     L + + G ++ P   SN D    S +KFDRRDVLFGLGGLYG A+L+     
Sbjct: 15  QIKNQTSNLQNKSRGINQVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTNQQSP 74

Query: 104 ALADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITP 163
           A A     ++PD  NC  A         CCPPKIHDIIDFI PP     R+RP AH +TP
Sbjct: 75  ASA---APLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTP 134

Query: 164 EQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFP 223
           +Q   FEKAI++MK LP +DPR F  QA VHCAYC+YAYKQL   P AE++VHWNALFFP
Sbjct: 135 KQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFP 194

Query: 224 FHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPP 283
           FHR YLYFFERILG+LI++P F IPFWNWD+  GM+MP+IY++P+S LYD+LR+ RH P 
Sbjct: 195 FHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPH 254

Query: 284 KLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIE 343
           KL  LNY GD++P +DVVD NLRWM+KQM     +A GF G+L+    EA +  GMG+IE
Sbjct: 255 KLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIE 314

Query: 344 SSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYT 403
            SPHNN+H+WVGDPN+ ++ +MG FYSA    +F+       ++          K RD+ 
Sbjct: 315 KSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKARDFD 374

Query: 404 DPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSP 463
           +PDWL+SSFV Y+E+K AVRIKVRDC+D+  LGYVYQ VD+PWL+T+Q PR      ++ 
Sbjct: 375 NPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQ 434

Query: 464 EPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNV 523
            P +     + FP  LNS + T V RP++    ++K E+DEVLVIE IE D+   VHFNV
Sbjct: 435 APQV-----IKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNV 494

Query: 524 LVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVK 583
           LVNV+D+G DV P N EF G+F  +PH HNH   K SLR VITE+++ELG++D E++ V+
Sbjct: 495 LVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVR 554

Query: 584 IAPKLGGENVTIGGIKIELQP 591
           + PK+GG+ VTIGG+KIELQP
Sbjct: 555 LDPKVGGDKVTIGGMKIELQP 561

BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 454.1 bits (1167), Expect = 2.4e-126
Identity = 266/550 (48.36%), Postives = 340/550 (61.82%), Query Frame = 0

Query: 66  SNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPA 125
           +N D+   + +K DRR+VL GLGGLYGVA + G D FA A       PD + CGPA  P 
Sbjct: 51  TNNDQNDQAQSKLDRRNVLLGLGGLYGVAGM-GTDPFAFAKP--IAPPDVSKCGPADLPQ 110

Query: 126 G--PRNCCPPKIHDIIDF-IPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDP 185
           G  P NCCPP    IIDF +P P     R+RP AH +      K+ KA+ LMK LP DDP
Sbjct: 111 GAVPTNCCPPPSTKIIDFKLPAP--AKLRIRPPAHAVDQAYRDKYYKAMELMKALPDDDP 170

Query: 186 RRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPT 245
           R F  QA VHCAYC+ AY Q+   P+ EL++H + LFFPFHR YLYFFE+ILG LI+DPT
Sbjct: 171 RSFKQQAAVHCAYCDGAYDQV-GFPELELQIHNSWLFFPFHRYYLYFFEKILGKLINDPT 230

Query: 246 FAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDED--PPFDVVD 305
           FA+PFWNWDSP GM +P IY +P S LYD+LR A H PP L  L+Y+G ED       ++
Sbjct: 231 FALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKETTIN 290

Query: 306 CNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNE 365
            NL+ M +QM    K+A+ F G   R G+E    G GSIE +PH  +H W GD   P+ E
Sbjct: 291 ANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGG-GSIEGTPHAPVHLWTGDNTQPNFE 350

Query: 366 DMGTFYSAG---MFFDSSNECMK-------LETKRRDYTDPDWLDSSFVLYDENKNAVRI 425
           DMG FYSAG   +FF   +   +       L  KR D TD DWLDS F+ Y+EN   VR+
Sbjct: 351 DMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAELVRV 410

Query: 426 KVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPL-------APPPEVY--- 485
           KVRDC+++ NLGYVYQDVDIPWL+++  PR+     S     L       A   +V    
Sbjct: 411 KVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKKLGVAHAAVASSSKVVAGT 470

Query: 486 -FPAMLNSEINTVVGRP-SRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGL 545
            FP  L S+I+TVV RP  + R+ + K +E+E+LVIE IEFDR   V F+V VN +D+ L
Sbjct: 471 EFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKFDVYVNDVDD-L 530

Query: 546 DVSPANIEFAGSFRNIPHAHNHNNAKNS-LRFVITELIEELGLDDAETLHVKIAPKLGGE 588
              P   EFAGSF ++PH+H H    N+ LR  +T+L+EE+  +D +++ V + PK G  
Sbjct: 531 PSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVVVTLVPKFGA- 590

BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 442.2 bits (1136), Expect = 9.3e-123
Identity = 264/581 (45.44%), Postives = 340/581 (58.52%), Query Frame = 0

Query: 32  FKIVAEGPAIGDEIRRSHRKLASYNNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLY 91
           F+  +  P   +  RR      S N+ +  P   S  D    S  K DRR+VL G+GGLY
Sbjct: 29  FQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVLLGIGGLY 88

Query: 92  GVASLSGGDRFALADDTHTISPDTANCGPARTPAG--PRNCCPPKIHDIIDFIPPPMIPH 151
           G A   G  +  LA      +PD + CG A  P G  P NCCPP    IIDF  P     
Sbjct: 89  GAAGGLGATK-PLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQLPSSGSP 148

Query: 152 PRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKA 211
            R RPAAHL++ E LAK++KAI L K LP DDPR F  QA VHC YC  AY Q+      
Sbjct: 149 MRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQV-GYTDL 208

Query: 212 ELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSL 271
           EL+VH + LF PFHR YLYF ERIL  LIDDPTFA+P+W WD+P GM MP IY +  SSL
Sbjct: 209 ELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYASSPSSL 268

Query: 272 YDRLRDARHVPPKLAYLNYDGDED--PPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRR 331
           YD  R+A+H+PP +  L+YDG E   P  ++   NL  M KQ+     + + FLG   R 
Sbjct: 269 YDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLGYPYRA 328

Query: 332 GEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--- 391
           G +A   G G++E +PHN +H W G  + P +EDMG FY+AG   +FF       ++   
Sbjct: 329 G-DAIDPGAGTLEHAPHNIVHKWTGLADKP-SEDMGNFYTAGRDPIFFGHHANVDRMWNI 388

Query: 392 -----ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWL--N 451
                   R+D+TD DWLD++FV YDENK  V++KV DCVD+  L Y YQD+ IPWL  N
Sbjct: 389 WKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPWLPKN 448

Query: 452 TRQIPRQNPPTSSSPEPPLAPPPEVY------FPAMLNSEINTVVGRPSRSRTIEQKGEE 511
           T+   +    +S S     A  P+        FP  LNS I   V RP +SR+ ++K +E
Sbjct: 449 TKAKAKTTTKSSKSGVAKAAELPKTTISSIGDFPKALNSVIRVEVPRPKKSRSKKEKEDE 508

Query: 512 DEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVS-PANIEFAGSFRNIPHAH-NHNNAKNS 571
           +EVL+I+ IE DR + V F+V +N  DE   VS P N EFAGSF N+PH H      K +
Sbjct: 509 EEVLLIKGIELDRENFVKFDVYIN--DEDYSVSRPKNSEFAGSFVNVPHKHMKEMKTKTN 568

Query: 572 LRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIE 588
           LRF I EL+E+LG +D E++ V I P+ GG++VTIGGI+IE
Sbjct: 569 LRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIE 603

BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match: Q6UIL3 ((+)-larreatricin hydroxylase, chloroplastic OS=Larrea tridentata OX=66636 PE=1 SV=1)

HSP 1 Score: 398.3 bits (1022), Expect = 1.5e-109
Identity = 235/557 (42.19%), Postives = 327/557 (58.71%), Query Frame = 0

Query: 53  ASYNNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTH-TI 112
           AS +N D     K N  E   S+   DRR++L GLGGLYG +++   ++  LA      +
Sbjct: 35  ASNDNKD-----KPNDQEKTFSI---DRRNMLIGLGGLYGASNVFPSNQSTLAAPIQPPV 94

Query: 113 SPDTANCGPARTPAGPRN--CCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEK 172
            PD  +C P    A   N  CCPP + D IDF  P      R+RPAAHL  P  + K++K
Sbjct: 95  LPD--SCHPPEDLAEGVNVLCCPPDVKDPIDFQMPSNPSRLRIRPAAHLADPTYIEKYKK 154

Query: 173 AIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYF 232
           A+  MK LP +DPR F  QA +HCAYCN AY Q+   P   ++VH + LF PFHR YLYF
Sbjct: 155 ALAAMKALPQNDPRSFYQQANIHCAYCNGAYDQV-GFPDVNIQVHHSWLFLPFHRWYLYF 214

Query: 233 FERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYD 292
           +ERILG LIDDPTFAIPFWNWD+P GM MP ++ +P+S LYD  R+  H P  +  L++ 
Sbjct: 215 YERILGSLIDDPTFAIPFWNWDAPKGMHMPHMFIDPNSPLYDAKRNPAHFPDTIVDLDFS 274

Query: 293 GDEDPPFD--VVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNL 352
             E P  +   +  NL  M KQ+ V  K AR F G+ ++ G     +G GS+E +PH N+
Sbjct: 275 SGEAPSHNPRQIGNNLSIMYKQV-VRAKKARLFHGRPLQAGSFPDESGDGSLEGTPHGNI 334

Query: 353 HDWVGDPNSPHNEDMGTFYSAG---MFF------DSSNECMKLETKRRDYTDPDWLDSSF 412
           H W GDP   + E+MG FYSAG   +F+      D      K    R+DY   DWL++ F
Sbjct: 335 HLWSGDPRQSNFENMGNFYSAGRDPLFYAHHANVDRMWYIWKNSLGRKDYKKKDWLNAGF 394

Query: 413 VLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPE 472
           +L+DEN   VR+ VRD +D   LGY YQ+VDIPW+N++  PR+  P  +          +
Sbjct: 395 LLFDENAQPVRVYVRDALDERKLGYAYQEVDIPWINSKPKPRKANPWPNRLRSKATTTTK 454

Query: 473 VY--FPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDE 532
           +   FP  L+S ++  V RP +SR+  +K +E+EVLVIE I+ +    + F+V +N  DE
Sbjct: 455 LINKFPLTLDSTVSFEVKRPKKSRSKSEKEDEEEVLVIEKIKHEPQFPLKFDVYIN--DE 514

Query: 533 GLDVSPAN-IEFAGSFRNIPHAHNHNNAKN-----SLRFVITELIEELGLDDAETLHVKI 588
             D S A+  EFAGSF N+PH H H + K+     +L   I+E+++EL +D  +++ V +
Sbjct: 515 DEDPSAADQTEFAGSFVNVPHFHRHGDKKDKRQTTNLSIGISEVLDELDVDGDDSIVVTL 574

BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 2.0e-109
Identity = 247/580 (42.59%), Postives = 339/580 (58.45%), Query Frame = 0

Query: 44  EIRRSHRKLASYNNGDETPIIKSNP-DEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
           ++R+  R++   +N ++     +NP +E  +S     RR+VL GLGG+YG  + +     
Sbjct: 38  KLRKPKRQVTCSSNNNQ-----NNPKEEQELSNIVGHRRNVLIGLGGIYGTLATNPS--- 97

Query: 104 ALADDTHTISPDTANC-GPARTPAGPR----NCCPPKIHDIIDFIPPPMIPHPRVRPAAH 163
           ALA       PD + C  P+  P+G      NCCPP    I DF  P   P  RVR AAH
Sbjct: 98  ALASPIS--PPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQP-LRVRQAAH 157

Query: 164 LITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNA 223
           L+  E L K++KA  LMK LP +DPR F+ QA +HCAYC+ AY Q+   P  +L+VH + 
Sbjct: 158 LVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQI-GFPDLKLQVHGSW 217

Query: 224 LFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDAR 283
           LFFPFHR YLYF+ERILG LI+DPTFA+PFWN+D+P GM++P IY +  S LYD LR+A 
Sbjct: 218 LFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDELRNAS 277

Query: 284 HVPPKLAYLNY--DGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAG 343
           H PP L  LN+   G +    +++  NL  M +Q+   GK++R FLG   R G +A+  G
Sbjct: 278 HQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAG-DAEPQG 337

Query: 344 MGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMK-------LETKR 403
            GSIE+ PH  +H W GD    + EDMG FYSA    +F+   +   +       L  K+
Sbjct: 338 AGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTLGGKK 397

Query: 404 RDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPT 463
            D+TD DWL+S F+ YDENKN VR+ V+D +D   LGY YQDV IPW   + +PR+    
Sbjct: 398 HDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRRTKVQ 457

Query: 464 S------------SSPEPPL----APPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEED 523
                         SP   L    +P   V FP +LN++++ +V RP + R+ ++K EE+
Sbjct: 458 KLVEVEVNDGNLRKSPTIFLVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKEKEEEE 517

Query: 524 EVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPH-AHNHNNAK--NS 583
           EVLVIE IEF  +  + F+V +N  DE   V   N EFAGSF NIPH AH H N K   S
Sbjct: 518 EVLVIEGIEFYMNIAIKFDVYIN--DEDDKVGAGNTEFAGSFVNIPHSAHGHKNKKIITS 577

Query: 584 LRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKI 587
           LR  IT+L+E+L ++  + + V + PK G   V I  ++I
Sbjct: 578 LRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEI 602

BLAST of Moc03g27430 vs. ExPASy Swiss-Prot
Match: Q9FRX6 (Aureusidin synthase OS=Antirrhinum majus OX=4151 GN=AS1 PE=1 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 4.9e-108
Identity = 227/521 (43.57%), Postives = 309/521 (59.31%), Query Frame = 0

Query: 89  GLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAG--PRNCCPPKIHDIIDFIPPPM 148
           GLY   SL+   RFA        +PD   CG    P G  P NCCPP    IIDF  PP 
Sbjct: 43  GLYIANSLAYA-RFASTSTGPIAAPDVTKCGQPDLPPGTAPINCCPPIPAKIIDFELPPP 102

Query: 149 IPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSV 208
               RVR AAHL+    +AKF+KA+ LM+ LP DDPR F  QA VHCAYC  AY Q    
Sbjct: 103 STTMRVRRAAHLVDDAYIAKFKKAVELMRALPEDDPRSFKQQANVHCAYCAGAYNQA-GF 162

Query: 209 PKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPD 268
              +L++H + LFFPFHR Y+YFFERILG LI+D TFA+PFWN+DSP GM +P ++ + +
Sbjct: 163 TNLKLQIHRSWLFFPFHRYYIYFFERILGKLINDTTFALPFWNYDSPGGMTIPSMFIDTN 222

Query: 269 SSLYDRLRDARHVPPKLAYLNY---DGDE-DPPFDVVDCNLRWMNKQMKVAGKSARGFLG 328
           SSLYD LRD+ H PP +  LNY   D D    P + +  NL+ + +QM  + K+ + F G
Sbjct: 223 SSLYDSLRDSNHQPPTIVDLNYAFSDSDNTTTPEEQMIINLKIVYRQMVSSAKTPQLFFG 282

Query: 329 QLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECM 388
           +  RRG++ +  G+GSIE  PH  +H W G  N+P+ E+MG FYS     +FF   +   
Sbjct: 283 RPYRRGDQ-EFPGVGSIELVPHGMIHLWTGSENTPYGENMGAFYSTARDPIFFAHHSNVD 342

Query: 389 KLET--------KRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIP 448
           ++ +        +R D TDPD+LD+SFV YDEN   VR+KVRDC+D   LGYVYQDV+IP
Sbjct: 343 RMWSIWKTLGGPRRTDLTDPDFLDASFVFYDENAEMVRVKVRDCLDEKKLGYVYQDVEIP 402

Query: 449 WLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEV 508
           WLNTR  P+ +P             P   FPA+L+  +  +V RP ++R+ ++K E +E+
Sbjct: 403 WLNTRPTPKVSPSLLKKFHRTNTANPRQVFPAILDRVLKVIVTRPKKTRSRKEKDELEEI 462

Query: 509 LVIEDIEFDRS-SRVHFNVLVNVLDEGLDV-SPANIEFAGSFRNIPH--AHNHNNAKNSL 568
           LVIE IE +R    V F+V +N  ++ L V SP N EFAGSF ++ H            L
Sbjct: 463 LVIEGIELERDHGHVKFDVYINADEDDLAVISPENAEFAGSFVSLWHKPIKGKRTKTQLL 522

Query: 569 RFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIEL 589
              I +++E+L  D+ + + V + P+  G+ + I  +KIEL
Sbjct: 523 TLSICDILEDLDADEDDYVLVTLVPRNAGDAIKIHNVKIEL 560

BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match: A0A172DC01 (Polyphenol oxidase OS=Luffa aegyptiaca OX=3670 GN=PPO2 PE=2 SV=1)

HSP 1 Score: 599.4 bits (1544), Expect = 1.7e-167
Identity = 329/552 (59.60%), Postives = 387/552 (70.11%), Query Frame = 0

Query: 56  NNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDT 115
           +NG +TP + SNP E G S++KFDRRDVLFGLGGLY  A+L G DR ALAD    ISPD 
Sbjct: 38  SNGVQTP-MNSNPQE-GDSMSKFDRRDVLFGLGGLYSAAAL-GRDRSALAD---PISPDV 97

Query: 116 ANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLM 175
            +C P    A  + +CCPP I DI DF+ PP + + RVRPAAHLIT EQLAK E+A+  M
Sbjct: 98  IHCEPITLEADEKVDCCPPSILDITDFV-PPAVENLRVRPAAHLITQEQLAKLEEAVTKM 157

Query: 176 KHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERIL 235
           K L  DDPR F  QA+VHCAYCN AYKQ D  P+ + ++HWN LFFPFHRCYLYF ERIL
Sbjct: 158 KALDPDDPRNFYQQARVHCAYCNDAYKQ-DGFPELDFDIHWNWLFFPFHRCYLYFHERIL 217

Query: 236 GYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPD--SSLYDRLRDARHVPPKLAYLNYDG-D 295
           G LI+DP FAIPFWNWD+ PGM+MP IY N D  S LYD LRD  H PPKL  LNYDG  
Sbjct: 218 GSLINDPDFAIPFWNWDAIPGMQMPAIYINDDKNSPLYDELRDPNHKPPKLLDLNYDGAT 277

Query: 296 EDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWV 355
           EDP +DV+ CN R M +QM +  K+A GF G+ +R G+EA   GMGSIE S HNN+H W 
Sbjct: 278 EDPSYDVLFCNPRLMYRQMVINSKTAEGFHGKPLRAGQEAYRTGMGSIELSAHNNVHTWS 337

Query: 356 GDPNSPHNEDMGTFYSAG---MFFDSSNECMKLET--------KRRDYTDPDWLDSSFVL 415
           GDP   + EDMG FY+A    +F+       ++ T        K RD+TDPDWL++SFV 
Sbjct: 338 GDPQQKYREDMGHFYAAARDPLFYAHHANVDRMWTIWQGLIDGKSRDFTDPDWLNASFVF 397

Query: 416 YDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVY 475
           YDENKNA+RIKV+DC+D+  LGY YQ V+IPWL+T+ IPRQ      S +    PP EV 
Sbjct: 398 YDENKNAIRIKVQDCLDNEKLGYKYQAVEIPWLHTKPIPRQKTSQLKSSKAQETPPIEV- 457

Query: 476 FPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDV 535
               LNS++   V RP RSR+  QK  EDEVLVIE +E D +SRVHFNVLVNVLD+G DV
Sbjct: 458 ----LNSKMTKEVKRPQRSRSKTQKSVEDEVLVIE-MELDTTSRVHFNVLVNVLDDGTDV 517

Query: 536 SPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVT 593
           SP N EFAGSF++IP  H H  A  SL  VITELIEELG DD ET+ V I PKLGG+ VT
Sbjct: 518 SPGNTEFAGSFKSIPLGHKH-RANTSLSLVITELIEELGYDDDETVFVTIDPKLGGDLVT 574

BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match: A0A6J1K2N9 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111490108 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 8.6e-164
Identity = 298/533 (55.91%), Postives = 379/533 (71.11%), Query Frame = 0

Query: 72  GISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRN-- 131
           G S +KFDRRDVLFGLGGLYG A+L+     A A     ++PD  NC PA T  G  +  
Sbjct: 40  GDSTSKFDRRDVLFGLGGLYGAAALTNQQSPASA---KPLAPDVNNCEPA-TIVGNEHIP 99

Query: 132 CCPPKIHDIIDFIPPPMIPHPRVRPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQA 191
           CCPPKIHDIIDFI PP     R+R  AH +TP+Q   FEKAI++MK L  +DPR F  QA
Sbjct: 100 CCPPKIHDIIDFIHPPHTTKLRIRQPAHSLTPKQQFNFEKAIHIMKRLSPNDPRNFYRQA 159

Query: 192 QVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWN 251
            VHCAYC+YAYKQL   P AE++VHWNALFFPFHR YLYFFERILG+LI++P FAIPFWN
Sbjct: 160 DVHCAYCDYAYKQL-GFPNAEMQVHWNALFFPFHRAYLYFFERILGHLINEPDFAIPFWN 219

Query: 252 WDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDGDEDPPFDVVDCNLRWMNKQ 311
           WD+  GM+MP+IY++P+S LYD+LR+ RH P KL  LNY GD++PP+DVVDCNLRWM+KQ
Sbjct: 220 WDNKKGMQMPEIYDHPNSPLYDKLRNQRHRPHKLLNLNYHGDDNPPYDVVDCNLRWMHKQ 279

Query: 312 MKVAGKSARGFLGQLIRRGEEA-KTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSA 371
           M     +A GF G+L+   +EA +  GMG+IE SPHNN+H+WVGDPN+ +N +MG FYSA
Sbjct: 280 MITDADTAEGFHGKLLVASQEANEETGMGTIERSPHNNIHNWVGDPNAKNNMNMGVFYSA 339

Query: 372 G---MFFDSSNECMKL--------ETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVD 431
               +F+       ++          K RD+ +PDWL+SSFV Y+E+K AVRIKV DC+D
Sbjct: 340 ARDPLFYGHHANVDRMWSIWQGLKNGKPRDFNNPDWLNSSFVFYNEHKQAVRIKVSDCLD 399

Query: 432 SVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPS 491
           +  LGYVYQ VD+PWL+T+Q PR      ++  P +     + FP  LNS + T V RP 
Sbjct: 400 TRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQAPQV-----IKFPQGLNSMVTTTVKRPK 459

Query: 492 RSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHA 551
           + R  ++K E+DEVLVIE IE D+   VHFNVLVNV+D+G DV P N EF G+F  + H 
Sbjct: 460 KLRDEKEKSEKDEVLVIEGIELDKRYWVHFNVLVNVVDDGSDVCPGNTEFVGTFTTVLHG 519

Query: 552 HNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQP 591
           HNH   K SLR VITE+++ELG++D E++ V++ PK+GG+ VTIGG+KIELQP
Sbjct: 520 HNH-RGKTSLRVVITEVLKELGIEDDESVIVRLDPKVGGDKVTIGGMKIELQP 561

BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match: A0A6J1GRK5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111456826 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.9e-163
Identity = 300/561 (53.48%), Postives = 387/561 (68.98%), Query Frame = 0

Query: 44  EIRRSHRKLASYNNG-DETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRF 103
           +I+     L + + G ++ P   SN D    S +KFDRRDVLFGLGGLYG A+L+     
Sbjct: 15  QIKNQTSNLQNKSRGINQVPCRASNGD----STSKFDRRDVLFGLGGLYGAAALTNQQSP 74

Query: 104 ALADDTHTISPDTANCGPARTPAGPR-NCCPPKIHDIIDFIPPPMIPHPRVRPAAHLITP 163
           A A     ++PD  NC  A         CCPPKIHDIIDFI PP     R+RP AH +TP
Sbjct: 75  ASA---APLAPDVNNCEAATIEGNEHIPCCPPKIHDIIDFIHPPHTTKLRIRPPAHSLTP 134

Query: 164 EQLAKFEKAIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFP 223
           +Q   FEKAI++MK LP +DPR F  QA VHCAYC+YAYKQL   P AE++VHWNALFFP
Sbjct: 135 KQQFNFEKAIHIMKRLPPNDPRNFYRQADVHCAYCDYAYKQL-GFPNAEMQVHWNALFFP 194

Query: 224 FHRCYLYFFERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPP 283
           FHR YLYFFERILG+LI++P F IPFWNWD+  GM+MP+IY++P+S LYD+LR+ RH P 
Sbjct: 195 FHRAYLYFFERILGHLINEPNFVIPFWNWDNTEGMQMPEIYDHPNSPLYDKLRNQRHRPH 254

Query: 284 KLAYLNYDGDEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEA-KTAGMGSIE 343
           KL  LNY GD++P +DVVD NLRWM+KQM     +A GF G+L+    EA +  GMG+IE
Sbjct: 255 KLLNLNYHGDDNPSYDVVDSNLRWMHKQMITDANTAEGFHGKLLIASHEANEETGMGTIE 314

Query: 344 SSPHNNLHDWVGDPNSPHNEDMGTFYSAG---MFFDSSNECMKL--------ETKRRDYT 403
            SPHNN+H+WVGDPN+ ++ +MG FYSA    +F+       ++          K RD+ 
Sbjct: 315 KSPHNNIHNWVGDPNAKNHMNMGAFYSAARDPLFYGHHANVDRMWSIWQGLKNGKARDFD 374

Query: 404 DPDWLDSSFVLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNPPTSSSP 463
           +PDWL+SSFV Y+E+K AVRIKVRDC+D+  LGYVYQ VD+PWL+T+Q PR      ++ 
Sbjct: 375 NPDWLNSSFVFYNEHKQAVRIKVRDCLDTRRLGYVYQYVDLPWLHTKQTPRLKKQLKTTQ 434

Query: 464 EPPLAPPPEVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNV 523
            P +     + FP  LNS + T V RP++    ++K E+DEVLVIE IE D+   VHFNV
Sbjct: 435 APQV-----IKFPQGLNSMVTTTVKRPNKLMNEKEKSEKDEVLVIEGIELDKRFWVHFNV 494

Query: 524 LVNVLDEGLDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVK 583
           LVNV+D+G DV P N EF G+F  +PH HNH   K SLR VITE+++ELG++D E++ V+
Sbjct: 495 LVNVVDDGSDVCPGNTEFVGTFTTVPHGHNH-RGKTSLRVVITEVLKELGIEDDESVIVR 554

Query: 584 IAPKLGGENVTIGGIKIELQP 591
           + PK+GG+ VTIGG+KIELQP
Sbjct: 555 LDPKVGGDKVTIGGMKIELQP 561

BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match: A0A5A7UM88 (Polyphenol oxidase I OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00520 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 9.9e-160
Identity = 304/554 (54.87%), Postives = 382/554 (68.95%), Query Frame = 0

Query: 51  KLASYNNGDETPIIKSNPDEVGISVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHT 110
           K AS NNG++             SV+KFDRR+VLFGLGGLYGV + +G    A       
Sbjct: 37  KAASSNNGED-------------SVSKFDRREVLFGLGGLYGVVTATGSVLAA------P 96

Query: 111 ISPDTANCGPARTPAGPRNCCPPKIHDIIDFIPPPMIPHPRV-RPAAHLITPEQLAKFEK 170
           ++PD  NC  A+TP G +NCCPP+I  II F+ PP I  P V RPAAHL+   Q+ + E+
Sbjct: 97  LTPDVFNCDMAQTPGGIKNCCPPRIGKIIHFV-PPKIQQPYVRRPAAHLMNQTQIEELER 156

Query: 171 AIYLMKHLPYDDPRRFSNQAQVHCAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYF 230
            I  +K L  DDP  FS+QA++HCAYC+YAY QL S+ +   EVH NALFFPFHR YLYF
Sbjct: 157 GIQKLKQLDEDDPHSFSHQAKIHCAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYF 216

Query: 231 FERILGYLIDDPTFAIPFWNWDSPPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYD 290
           FERIL + I +P FAIPFWNWDSPPGM MP+ YN P SSLYD LRD RH P KL  LNY+
Sbjct: 217 FERILRHYIGNPDFAIPFWNWDSPPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYN 276

Query: 291 G-DEDPPFDVVDCNLRWMNKQMKVAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLH 350
           G  ED  FDVVDCNLRWMN +MKV  +S R FLG++++ GE  K + MG+IE SPHNN+H
Sbjct: 277 GKTEDHSFDVVDCNLRWMNDRMKV--QSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIH 336

Query: 351 DWVGDPNSPHNEDMGTFYSAG---MFFDSS-------NECMKLETKRRDYTDPDWLDSSF 410
           DWVG   +PH EDMG+FYSA    +F+          N    L+   +D+TDPDWL+SSF
Sbjct: 337 DWVG-TKTPHFEDMGSFYSAALDPLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSF 396

Query: 411 VLYDENKNAVRIKVRDCVDSVNLGYVYQDVDIPWLNTRQIPRQNP-PTSSSPEPPLAPPP 470
           V YDENKNAVRI+V DC+D+  LGYVYQ+V +PWL   +IP  NP   +  PEPP+    
Sbjct: 397 VFYDENKNAVRIEVSDCLDTEKLGYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPI---- 456

Query: 471 EVYFPAMLNSEINTVVGRPSRSRTIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEG 530
            + FP  LNS+I T+V RP  + + E   E D VLV+ED+E+D ++RVHF+VLVNV+DE 
Sbjct: 457 -INFPQTLNSDITTMVDRPEEAGSGE---EVDVVLVLEDVEYDPTTRVHFDVLVNVVDEA 516

Query: 531 LDVSPANIEFAGSFRNIPHAHNHNNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGE 590
            D   A +EF GSF+NIPH  +H++ K SLRFVITE+I+ LGL+  + L VKI P +GGE
Sbjct: 517 QDRCAAFLEFVGSFKNIPHGLHHHD-KTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGE 558

Query: 591 NVTIGGIKIELQPI 592
            VT+GGIKI+L+P+
Sbjct: 577 GVTLGGIKIQLEPV 558

BLAST of Moc03g27430 vs. ExPASy TrEMBL
Match: A0A1S3BFJ5 (polyphenol oxidase I, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103489300 PE=3 SV=1)

HSP 1 Score: 570.5 bits (1469), Expect = 8.3e-159
Identity = 298/531 (56.12%), Postives = 374/531 (70.43%), Query Frame = 0

Query: 74  SVNKFDRRDVLFGLGGLYGVASLSGGDRFALADDTHTISPDTANCGPARTPAGPRNCCPP 133
           SV+KFDRR+VLFGLGGLYGV + +G    A       ++PD  NC  A+TP G +NCCPP
Sbjct: 212 SVSKFDRREVLFGLGGLYGVVTATGSVLAA------PLTPDVFNCDMAQTPGGIKNCCPP 271

Query: 134 KIHDIIDFIPPPMIPHPRV-RPAAHLITPEQLAKFEKAIYLMKHLPYDDPRRFSNQAQVH 193
           +I  II F+ PP I  P V RPAAHL+   Q+ + E+ I  +K L  DDP  FS+QA++H
Sbjct: 272 RIGKIIHFV-PPKIQQPYVRRPAAHLMNQTQIEELERGIQKLKQLDEDDPHSFSHQAKIH 331

Query: 194 CAYCNYAYKQLDSVPKAELEVHWNALFFPFHRCYLYFFERILGYLIDDPTFAIPFWNWDS 253
           CAYC+YAY QL S+ +   EVH NALFFPFHR YLYFFERIL + I +P FAIPFWNWDS
Sbjct: 332 CAYCDYAYNQLGSITETRFEVHSNALFFPFHRAYLYFFERILRHYIGNPDFAIPFWNWDS 391

Query: 254 PPGMKMPKIYNNPDSSLYDRLRDARHVPPKLAYLNYDG-DEDPPFDVVDCNLRWMNKQMK 313
           PPGM MP+ YN P SSLYD LRD RH P KL  LNY+G  ED  FDVVDCNLRWMN +MK
Sbjct: 392 PPGMHMPEFYNRPSSSLYDNLRDPRHDPHKLIDLNYNGKTEDHSFDVVDCNLRWMNDRMK 451

Query: 314 VAGKSARGFLGQLIRRGEEAKTAGMGSIESSPHNNLHDWVGDPNSPHNEDMGTFYSAG-- 373
           V  +S R FLG++++ GE  K + MG+IE SPHNN+HDWVG   +PH EDMG+FYSA   
Sbjct: 452 V--QSPRSFLGRVLKAGETPKPSDMGTIEQSPHNNIHDWVG-TKTPHFEDMGSFYSAALD 511

Query: 374 -MFFDSS-------NECMKLETKRRDYTDPDWLDSSFVLYDENKNAVRIKVRDCVDSVNL 433
            +F+          N    L+   +D+TDPDWL+SSFV YDENKNAVRI+V DC+D+  L
Sbjct: 512 PLFYAHHTNIDRLWNIWKTLDGNPKDHTDPDWLNSSFVFYDENKNAVRIEVSDCLDTEKL 571

Query: 434 GYVYQDVDIPWLNTRQIPRQNP-PTSSSPEPPLAPPPEVYFPAMLNSEINTVVGRPSRSR 493
           GYVYQ+V +PWL   +IP  NP   +  PEPP+     + FP  LNS+I T+V RP  + 
Sbjct: 572 GYVYQEVPLPWLLLNKIPSPNPWIRTEEPEPPI-----INFPQTLNSDITTMVDRPEEAG 631

Query: 494 TIEQKGEEDEVLVIEDIEFDRSSRVHFNVLVNVLDEGLDVSPANIEFAGSFRNIPHAHNH 553
           + E   E D VLV+ED+E+D ++RVHF+VLVNV+DE  D   A +EF GSF+NIPH  +H
Sbjct: 632 SGE---EVDVVLVLEDVEYDPTTRVHFDVLVNVVDEAQDRCAAFLEFVGSFKNIPHGLHH 691

Query: 554 NNAKNSLRFVITELIEELGLDDAETLHVKIAPKLGGENVTIGGIKIELQPI 592
           ++ K SLRFVITE+I+ LGL+  + L VKI P +GGE VT+GGIKI+L+P+
Sbjct: 692 HD-KTSLRFVITEVIQGLGLERDKNLAVKIEPMVGGEGVTLGGIKIQLEPV 723