Moc03g27390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g27390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	polyphenol oxidase, chloroplastic-like
Location	chr3: 19695705 .. 19697619 (+)
RNA-Seq Expression	Moc03g27390
Synteny	Moc03g27390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTCTGTGTGCCCTCCATTACCACCATCACTTCTCCCATCATTCAGAAGAAAGTTTGTTGTGAATAAAAATAATAAGCATTGTCGAGTTGTGTGCAGCGCCTCCAATAAGCATGATTCCCAGTCCCAGTCCCCAGGCAATATGGTGGACCGGAGAGACGTGCTTATTGGGCTGGGCGGGCTGTACGGGGCTTCCAGTTTTGCCCCAGAATCTGATTCCTTTGCCATTGCCGCTCCGGTGGACACCCCCCAAATTACCAAGTGCGGGCCAGCACAGGTGCGCGACAAGAAGGTCGAATGCTGCCCCCCGCCGTCCATCAAAATCAAAGACTTCCGGCCGACTCCACCCCGCAAATTACGAGTTCGGCCGGCCGCCCATTTAGTCGACAAAAACTACCTGAAAAAATATGAGAGAGCCGTGGAGTTGATGAGAGGCCTGCCAGACCATGACCCGCGTAGTTTCATGCAGCAGGCGAATATTCACTGTGCCTATTGTAACGGCGGTTACAAGCAACTTGGGTTCCCAGTTGAGCTCCAGGTTCATAATTCGTGGCTTTTTTTCCCTTTCCATCGCTTTTATCTCTACTTCCATGAGAAGATCTTGGGAGAGCTGATTGGGGACCCCACTTTCGCCTTGCCGTTTTGGAACTACGACGCTCCGGCGGGGATGACATTTCCGGCCATCTTCGCCAACCAGAACTCGCCGCTTTACGATGAATTTCGTAACGAGAATCATATGCCGCCGACGGTGGTGGATCTGGATTACAATGGCACCGATGTAAATATGGATGATGAGGCCAGAGTAAGGAGTAACCTTCGGGTTATGTATCGGCAGATGGTATCCGGCAGCCGTACGCGGTCACTGTTTTTCGGCCGCCCTTATCGAGCGGGCGACGACCCATCACCAGGTCACATTTTCCTAACCACATATTTTAAGCTTTTAACACTTTATCCTTTTTAAATTACAATCATATCTCAATTCTGTATCTCTTAGCTAGCGATAAGAGGTGTGAGGTGTTAATCAATCCTAGCCATGAATCTTGATTATAATAAATATTACAGGAGCTGGGAGCGTGGAGAACGTTCCTCACATCCCAATTCACAGATGGTGCGGCAACAAGCACCAGCCCAATGGTGAGGACATGGGAAACTTCTACTCCGCCGGTCGAGACCCGGTTTTCTACTCCCACCACGCAAACGTGGACCGGATGTGGTGCATCTGGAAAACCCTGGGCCGACGGCGCAGGGACATTCAAAACCCCGATTTCCTCAACGCATCTTTCGTGTTCTACAACGAGAAGAAGGAGGCCGTGCGAGTCCGCGTCCGGGACTGCCTCGACACCACAGCCCTCGGCTACGTCTACCAGGACGTGGACATTCCGTGGCTCAAAACTGCCCCCTCGCCACGTCGGAAGAAGCTTCGAAAGGGTGGCGTGGCCAATAACAATATGCCGAAATTCCCATCCTTGCTGGGCACCGGGGCAGCCCTGGCGGCGGAGTCGGAGTTGGAGTTTCCGCTGTCTCTGGAGTCGGTGATCAGGACGGACGTGAAGCGGCCGAAGAAGTCGAGGAGCAGCAAGGAGAAGGAGGAGGAAGAGGAGATTTTGGTGATCTCTGGGATCGAATTTGACCCCAACACGGCCGTGAAGTTCGACGTTTTGATCAACAGCGAAGAGGACAGGGAAATACGGGCGGATAACACTGAGTTTGCCGGCAGCTTCGCGAGCGTGCCCCATCAGAACGCCAACATGAACAACCTCAAGACCTGCCTTAGGTTGGGGATAACTGAACTGCTTGAAGATTTGGGCATAGAGGATCAAGACAGCGTCGTCGTCACTCTCGTGCCTAGGATCAAGGATCAGAACATTACCATTGGTGGCATCGAGATTGAATTTGACCAAGATGATGATTAA

mRNA sequence

ATGGCTTCTGTGTGCCCTCCATTACCACCATCACTTCTCCCATCATTCAGAAGAAAGTTTGTTGTGAATAAAAATAATAAGCATTGTCGAGTTGTGTGCAGCGCCTCCAATAAGCATGATTCCCAGTCCCAGTCCCCAGGCAATATGGTGGACCGGAGAGACGTGCTTATTGGGCTGGGCGGGCTGTACGGGGCTTCCAGTTTTGCCCCAGAATCTGATTCCTTTGCCATTGCCGCTCCGGTGGACACCCCCCAAATTACCAAGTGCGGGCCAGCACAGGTGCGCGACAAGAAGGTCGAATGCTGCCCCCCGCCGTCCATCAAAATCAAAGACTTCCGGCCGACTCCACCCCGCAAATTACGAGTTCGGCCGGCCGCCCATTTAGTCGACAAAAACTACCTGAAAAAATATGAGAGAGCCGTGGAGTTGATGAGAGGCCTGCCAGACCATGACCCGCGTAGTTTCATGCAGCAGGCGAATATTCACTGTGCCTATTGTAACGGCGGTTACAAGCAACTTGGGTTCCCAGTTGAGCTCCAGGTTCATAATTCGTGGCTTTTTTTCCCTTTCCATCGCTTTTATCTCTACTTCCATGAGAAGATCTTGGGAGAGCTGATTGGGGACCCCACTTTCGCCTTGCCGTTTTGGAACTACGACGCTCCGGCGGGGATGACATTTCCGGCCATCTTCGCCAACCAGAACTCGCCGCTTTACGATGAATTTCGTAACGAGAATCATATGCCGCCGACGGTGGTGGATCTGGATTACAATGGCACCGATGTAAATATGGATGATGAGGCCAGAGTAAGGAGTAACCTTCGGGTTATGTATCGGCAGATGGTATCCGGCAGCCGTACGCGGTCACTGTTTTTCGGCCGCCCTTATCGAGCGGGCGACGACCCATCACCAGGAGCTGGGAGCGTGGAGAACGTTCCTCACATCCCAATTCACAGATGGTGCGGCAACAAGCACCAGCCCAATGGTGAGGACATGGGAAACTTCTACTCCGCCGGTCGAGACCCGGTTTTCTACTCCCACCACGCAAACGTGGACCGGATGTGGTGCATCTGGAAAACCCTGGGCCGACGGCGCAGGGACATTCAAAACCCCGATTTCCTCAACGCATCTTTCGTGTTCTACAACGAGAAGAAGGAGGCCGTGCGAGTCCGCGTCCGGGACTGCCTCGACACCACAGCCCTCGGCTACGTCTACCAGGACGTGGACATTCCGTGGCTCAAAACTGCCCCCTCGCCACGTCGGAAGAAGCTTCGAAAGGGTGGCGTGGCCAATAACAATATGCCGAAATTCCCATCCTTGCTGGGCACCGGGGCAGCCCTGGCGGCGGAGTCGGAGTTGGAGTTTCCGCTGTCTCTGGAGTCGGTGATCAGGACGGACGTGAAGCGGCCGAAGAAGTCGAGGAGCAGCAAGGAGAAGGAGGAGGAAGAGGAGATTTTGGTGATCTCTGGGATCGAATTTGACCCCAACACGGCCGTGAAGTTCGACGTTTTGATCAACAGCGAAGAGGACAGGGAAATACGGGCGGATAACACTGAGTTTGCCGGCAGCTTCGCGAGCGTGCCCCATCAGAACGCCAACATGAACAACCTCAAGACCTGCCTTAGGTTGGGGATAACTGAACTGCTTGAAGATTTGGGCATAGAGGATCAAGACAGCGTCGTCGTCACTCTCGTGCCTAGGATCAAGGATCAGAACATTACCATTGGTGGCATCGAGATTGAATTTGACCAAGATGATGATTAA

Coding sequence (CDS)

ATGGCTTCTGTGTGCCCTCCATTACCACCATCACTTCTCCCATCATTCAGAAGAAAGTTTGTTGTGAATAAAAATAATAAGCATTGTCGAGTTGTGTGCAGCGCCTCCAATAAGCATGATTCCCAGTCCCAGTCCCCAGGCAATATGGTGGACCGGAGAGACGTGCTTATTGGGCTGGGCGGGCTGTACGGGGCTTCCAGTTTTGCCCCAGAATCTGATTCCTTTGCCATTGCCGCTCCGGTGGACACCCCCCAAATTACCAAGTGCGGGCCAGCACAGGTGCGCGACAAGAAGGTCGAATGCTGCCCCCCGCCGTCCATCAAAATCAAAGACTTCCGGCCGACTCCACCCCGCAAATTACGAGTTCGGCCGGCCGCCCATTTAGTCGACAAAAACTACCTGAAAAAATATGAGAGAGCCGTGGAGTTGATGAGAGGCCTGCCAGACCATGACCCGCGTAGTTTCATGCAGCAGGCGAATATTCACTGTGCCTATTGTAACGGCGGTTACAAGCAACTTGGGTTCCCAGTTGAGCTCCAGGTTCATAATTCGTGGCTTTTTTTCCCTTTCCATCGCTTTTATCTCTACTTCCATGAGAAGATCTTGGGAGAGCTGATTGGGGACCCCACTTTCGCCTTGCCGTTTTGGAACTACGACGCTCCGGCGGGGATGACATTTCCGGCCATCTTCGCCAACCAGAACTCGCCGCTTTACGATGAATTTCGTAACGAGAATCATATGCCGCCGACGGTGGTGGATCTGGATTACAATGGCACCGATGTAAATATGGATGATGAGGCCAGAGTAAGGAGTAACCTTCGGGTTATGTATCGGCAGATGGTATCCGGCAGCCGTACGCGGTCACTGTTTTTCGGCCGCCCTTATCGAGCGGGCGACGACCCATCACCAGGAGCTGGGAGCGTGGAGAACGTTCCTCACATCCCAATTCACAGATGGTGCGGCAACAAGCACCAGCCCAATGGTGAGGACATGGGAAACTTCTACTCCGCCGGTCGAGACCCGGTTTTCTACTCCCACCACGCAAACGTGGACCGGATGTGGTGCATCTGGAAAACCCTGGGCCGACGGCGCAGGGACATTCAAAACCCCGATTTCCTCAACGCATCTTTCGTGTTCTACAACGAGAAGAAGGAGGCCGTGCGAGTCCGCGTCCGGGACTGCCTCGACACCACAGCCCTCGGCTACGTCTACCAGGACGTGGACATTCCGTGGCTCAAAACTGCCCCCTCGCCACGTCGGAAGAAGCTTCGAAAGGGTGGCGTGGCCAATAACAATATGCCGAAATTCCCATCCTTGCTGGGCACCGGGGCAGCCCTGGCGGCGGAGTCGGAGTTGGAGTTTCCGCTGTCTCTGGAGTCGGTGATCAGGACGGACGTGAAGCGGCCGAAGAAGTCGAGGAGCAGCAAGGAGAAGGAGGAGGAAGAGGAGATTTTGGTGATCTCTGGGATCGAATTTGACCCCAACACGGCCGTGAAGTTCGACGTTTTGATCAACAGCGAAGAGGACAGGGAAATACGGGCGGATAACACTGAGTTTGCCGGCAGCTTCGCGAGCGTGCCCCATCAGAACGCCAACATGAACAACCTCAAGACCTGCCTTAGGTTGGGGATAACTGAACTGCTTGAAGATTTGGGCATAGAGGATCAAGACAGCGTCGTCGTCACTCTCGTGCCTAGGATCAAGGATCAGAACATTACCATTGGTGGCATCGAGATTGAATTTGACCAAGATGATGATTAA

Protein sequence

MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLGGLYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDQDDD

Homology

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BLAST of Moc03g27390 vs. NCBI nr
Match: XP_022139808.1 (polyphenol oxidase, chloroplastic-like [Momordica charantia])

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 584/586 (99.66%), Postives = 584/586 (99.66%), Query Frame = 0


Query: 1   MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG 60
           MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG
Sbjct: 9   MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG 68

Query: 61  GLYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKL 120
           GLYGASSFAPE DSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKL
Sbjct: 69  GLYGASSFAPEXDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKL 128

Query: 121 RVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQ 180
           RVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQ
Sbjct: 129 RVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQ 188

Query: 181 VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE 240
           VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE
Sbjct: 189 VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE 248

Query: 241 FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD 300
           FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD
Sbjct: 249 FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD 308

Query: 301 PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL 360
           PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL
Sbjct: 309 PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL 368

Query: 361 GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR 420
           GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR
Sbjct: 369 GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR 428

Query: 421 KKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEE 480
           KKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEE
Sbjct: 429 KKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEE 488

Query: 481 EEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCL 540
           EEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCL
Sbjct: 489 EEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCL 548

Query: 541 RLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDQDDD 587
           RLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD DDD
Sbjct: 549 RLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDPDDD 594

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BLAST of Moc03g27390 vs. NCBI nr
Match: XP_023001713.1 (polyphenol oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 742.7 bits (1916), Expect = 2.5e-210
Identity = 358/587 (60.99%), Postives = 449/587 (76.49%), Query Frame = 0


Query: 1   MASVCPPL--PPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIG 60
           MAS+ PP     +  PS R        N   RV+CSA+N            +DRRDVLIG
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAAN----------GKIDRRDVLIG 60

Query: 61  L--GGLYGASSFAPESDSFAIAAPVDTPQITKCGPAQV--RDKKVECCPPPSIKIKDFRP 120
           +  GG+YGAS+F  +S +FA+AAPV TP I+KCGP  +        CCPPPS  I DFRP
Sbjct: 61  IGAGGIYGASAFTKDS-TFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRP 120

Query: 121 TPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLG 180
             P KLRVRPAAHL D++Y+ KY+RAVELM+GLP  DPRSFMQQAN+HCAYCNGGY Q+G
Sbjct: 121 PAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVG 180

Query: 181 FPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQN 240
           FPVE+QVHNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+ N
Sbjct: 181 FPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHN 240

Query: 241 SPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRP 300
           S LYD+ RN+NH+PPT++DLD++G D N+ D A++RSNLR+MYRQMVS SRT  LFFG  
Sbjct: 241 SSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSA 300

Query: 301 YRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMW 360
           Y AGD+PSPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+NVDRMW
Sbjct: 301 YVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMW 360

Query: 361 CIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKT 420
            +WKTLG RRRD+Q P++L+ASF+FYNEK +AVRVRVRDCLDT  LGY+YQDV++PWL+T
Sbjct: 361 SVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLET 420

Query: 421 APSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRS 480
            P+PRR  LR     ++N+P         +      +++ P++L+S I T+VKR KKSR 
Sbjct: 421 RPTPRR-ALRAQKSVDSNVP---------SRRVKPPKMKLPVTLKSAISTEVKRGKKSRG 480

Query: 481 SKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMN 540
            KEK+EEEEILVI GIEFDPNT++K D+ IN E+DREIR DNTEFAGSF +VPH++ +  
Sbjct: 481 RKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKK 540

Query: 541 NLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 582
            +KT LRLGI+E+LEDL  +D DS++VT++PR+    ++I  I+IE+
Sbjct: 541 KVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 566

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BLAST of Moc03g27390 vs. NCBI nr
Match: XP_038903169.1 (polyphenol oxidase, chloroplastic-like [Benincasa hispida])

HSP 1 Score: 740.3 bits (1910), Expect = 1.2e-209
Identity = 358/590 (60.68%), Postives = 444/590 (75.25%), Query Frame = 0


Query: 9   PPSLLP------SFRRKFVVNKNNKHCRVV-CSASNKHDSQSQSPGNMVDRRDVLIGLGG 68
           PPSL        SF  K     NN   R++ C+  N +D  +  P   +DRRD+L+GL G
Sbjct: 7   PPSLTTYFPSSNSFLPKTKTLPNNISPRIISCNTVNHNDQNTPKP--KIDRRDILVGLSG 66

Query: 69  LYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRK 128
           LYGAS+    S +FA+AAP+ TP I+KCGPA +        CCPPP+ +I DF+P    K
Sbjct: 67  LYGASTLYKNS-NFALAAPIQTPNISKCGPADLPPGADSTNCCPPPATRILDFQPPAQEK 126

Query: 129 LRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVEL 188
           LRVRPAAHLVDKNY++KY++AVELM+ LP++DPRSF QQAN+HCAYCNG Y Q+GFPVE+
Sbjct: 127 LRVRPAAHLVDKNYIEKYKKAVELMKALPENDPRSFTQQANVHCAYCNGAYDQVGFPVEV 186

Query: 189 QVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYD 248
           QVHNSWLFFPFHR YLYFHEKILGELIGDP FALPFWNYDAP GM  P ++A + S LYD
Sbjct: 187 QVHNSWLFFPFHRLYLYFHEKILGELIGDPNFALPFWNYDAPGGMRMPEMYAEEKSSLYD 246

Query: 249 EFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD 308
           E RNENH+PP +VDLD+   D N+ D+A++RSNL +MYRQMVS SRT SLFFG  YRAGD
Sbjct: 247 ELRNENHVPPRLVDLDFGRVDPNIGDDAQIRSNLSIMYRQMVSNSRTPSLFFGSAYRAGD 306

Query: 309 DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKT 368
           +PSPG GS+EN+PH P+H WCG+ ++PNGE+MGNFYSA RDP+FY+HH+N+DR+W +WKT
Sbjct: 307 EPSPGGGSIENIPHGPVHVWCGDNNEPNGENMGNFYSAARDPIFYAHHSNIDRLWSVWKT 366

Query: 369 LGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPR 428
           LG RR+D+QNP++LN SF+FYNEK EAVRVRVR  LD+  LGYVYQDVD+PWL+T P+PR
Sbjct: 367 LGGRRQDLQNPEWLNTSFIFYNEKAEAVRVRVRQSLDSKILGYVYQDVDVPWLQTRPTPR 426

Query: 429 RKKLRKGGVANNNMPKFPSLLGTGAALAAES------ELEFPLSLESVIRTDVKRPKKSR 488
           R +     VANNN+   P  +G   A    S       ++FP++++S +  +VKRPKKSR
Sbjct: 427 RAQKT---VANNNI-ILPFSIGPALAAGPSSSRVRPANIKFPITVKSAVSVEVKRPKKSR 486

Query: 489 SSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANM 548
           S KEKEEEEEILVI GI FDPN  +KFDV IN E+D+EIR DNTEFAGSF +VPH+  + 
Sbjct: 487 SKKEKEEEEEILVIYGIGFDPNQGIKFDVFINDEDDKEIRPDNTEFAGSFVNVPHKLGHK 546

Query: 549 NNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDQ 584
             +KT LRLGITE+LEDL  +D DSV VT+VPR+     +I GI+I  D+
Sbjct: 547 KTVKTGLRLGITEILEDLEADDDDSVRVTIVPRLGAGLFSIDGIKIVCDK 589

[Close]

BLAST of Moc03g27390 vs. NCBI nr
Match: APO14262.1 (polyphenol oxidase 3 [Luffa aegyptiaca])

HSP 1 Score: 740.0 bits (1909), Expect = 1.6e-209
Identity = 352/565 (62.30%), Postives = 439/565 (77.70%), Query Frame = 0


Query: 25  NNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLGGLYGASSFAPESDSFAIAAPVDTP 84
           ++K  R++CSASN          N +DRRD+LIGLGGLYGAS+ +    S A AAPV TP
Sbjct: 38  SSKAPRILCSASN-------GGQNHIDRRDILIGLGGLYGASTLS--QGSLAFAAPVQTP 97

Query: 85  QITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVE 144
            I+KCG   +      + CCPPP+  + DFRP  P KLRVRPAAHL D+NY++KY+RAVE
Sbjct: 98  NISKCGQPDLPPGADPINCCPPPTTAVVDFRPQAPGKLRVRPAAHLADQNYVEKYKRAVE 157

Query: 145 LMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKIL 204
           LM+ LPD DPRSF QQAN+HCAYCNG Y Q+GFPVE+QVHNSWLFFPFHR+YL+FHEKIL
Sbjct: 158 LMKALPDDDPRSFKQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRYYLHFHEKIL 217

Query: 205 GELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVN 264
           GELIGDP+FALPFWNYDAP GM  PA++A++ S LYDE RN NH+PPT+VDLD+ G D  
Sbjct: 218 GELIGDPSFALPFWNYDAPGGMKMPAMYADEKSSLYDELRNRNHLPPTLVDLDFGGVDPT 277

Query: 265 MDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPHIPIHRWCGN 324
           + DEA++RSNL +MYRQMVS SRT SLFFG  Y AGD+PSPG G VEN+PH P+H WCG+
Sbjct: 278 IGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAYLAGDEPSPGGGLVENIPHGPVHIWCGD 337

Query: 325 KHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNE 384
            ++PN E+MGNFYSA RDP+FY+HH+N+DR+W +WKTLG RR+D+QN D+LNASF+FY+E
Sbjct: 338 NNEPNFENMGNFYSAARDPIFYAHHSNIDRLWSVWKTLGGRRQDLQNADWLNASFIFYDE 397

Query: 385 KKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLL-- 444
           K +AVRVRVRDCLD+  LGYVYQDV +PWL++ P+PRR    +  VA+      P+L   
Sbjct: 398 KAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESRPTPRRALRAQKAVAHLPFGVGPALAAG 457

Query: 445 --GTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAV 504
             G  ++    ++++FP++L S I  +VKRPK+SRS KEK+EEEEI+VI GIEFD N A+
Sbjct: 458 EPGPSSSRLRPAKIKFPITLNSPISMEVKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAI 517

Query: 505 KFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIEDQDS 564
           KFDV IN E+D+EIR DNTEFAGSF +VPH++ +  N+KT LRLGITELLEDL  +D DS
Sbjct: 518 KFDVFINDEDDKEIRPDNTEFAGSFVNVPHKHGHKKNVKTGLRLGITELLEDLDADDDDS 577

Query: 565 VVVTLVPRIKDQNITIGGIEIEFDQ 584
           V VTLVPR     ++I GI+IEFD+
Sbjct: 578 VRVTLVPRSGKGLVSIAGIKIEFDK 593

[Close]

BLAST of Moc03g27390 vs. NCBI nr
Match: XP_023519283.1 (polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 735.3 bits (1897), Expect = 4.0e-208
Identity = 350/582 (60.14%), Postives = 448/582 (76.98%), Query Frame = 0


Query: 9   PPSLLPSFRRKFVVNKNNKH-----CRVVCSASNKHDSQSQSPGNMVDRRDVLIGL--GG 68
           PP+L  +  R     + +K+      R++CSA++            +DRRDVLIG+  GG
Sbjct: 6   PPALSTAAFRPSSSLRTSKYLPINAARILCSAAD----------GKIDRRDVLIGIGAGG 65

Query: 69  LYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRK 128
           LYGAS+F  +S +FA+AAPV TP I+KCGP  +        CCPPP+  I DF P  P K
Sbjct: 66  LYGASAFTKDS-TFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIVDFLPPAPGK 125

Query: 129 LRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVEL 188
           LRVRPAAHL D++Y+ KY+RAVELM+GLP+ DPRSFMQQAN+HCAYCNGGY Q+GFPVE+
Sbjct: 126 LRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEV 185

Query: 189 QVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYD 248
           QVHNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+QNS LYD
Sbjct: 186 QVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYD 245

Query: 249 EFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD 308
           + RN+NH+PPT++DLD++G D N+ + A++R+NLR+MYRQMVS SRT  LFFG  Y AGD
Sbjct: 246 QLRNQNHLPPTLIDLDFDGVDSNIGNNAQIRTNLRIMYRQMVSNSRTPKLFFGSAYVAGD 305

Query: 309 DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKT 368
           +PSPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+N+DRMW +WKT
Sbjct: 306 EPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKT 365

Query: 369 LGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPR 428
           LG RR+D+Q P++L+ASF+FYNEK EAVRVRVRDCLDT  LGY+YQDV++PWL+T P+PR
Sbjct: 366 LGGRRQDLQYPEWLDASFIFYNEKGEAVRVRVRDCLDTKNLGYIYQDVELPWLETRPTPR 425

Query: 429 RKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKE 488
           R  LR     ++N+P         +       ++FP+++ES + T+VKR KKSRS KEK+
Sbjct: 426 R-ALRAQKSVDSNVP---------SRRVKPGRMKFPVTVESAVSTEVKRGKKSRSRKEKD 485

Query: 489 EEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTC 548
           EEEEILVI GIEFDPNTA+K DV IN E++REIR DNTEFAGSF +VPH++ +   +KT 
Sbjct: 486 EEEEILVIKGIEFDPNTAIKLDVYINDEDEREIRGDNTEFAGSFVNVPHKHGHKKKVKTG 545

Query: 549 LRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 582
           LRLGITE+LEDL  +D DS++VT++P +    ++I  I +E+
Sbjct: 546 LRLGITEVLEDLEADDDDSIIVTIIPSLGAGLVSIADITLEY 566

[Close]

BLAST of Moc03g27390 vs. ExPASy Swiss-Prot
Match: P43309 (Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 1.3e-190
Identity = 330/561 (58.82%), Postives = 409/561 (72.91%), Query Frame = 0


Query: 30  RVVCSASNKHDSQSQSPGNMVDRRDVLIGLGGLYGASSFAPESDSFAIAAPVDTPQITKC 89
           +V C A+N  + Q+    + +DRR+VL+GLGGLYG +     +D FA A P+  P ++KC
Sbjct: 45  KVSCKATN--NDQNDQAQSKLDRRNVLLGLGGLYGVAGMG--TDPFAFAKPIAPPDVSKC 104

Query: 90  GPAQVRDKKV--ECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGL 149
           GPA +    V   CCPPPS KI DF+   P KLR+RP AH VD+ Y  KY +A+ELM+ L
Sbjct: 105 GPADLPQGAVPTNCCPPPSTKIIDFKLPAPAKLRIRPPAHAVDQAYRDKYYKAMELMKAL 164

Query: 150 PDHDPRSFMQQANIHCAYCNGGYKQLGFP-VELQVHNSWLFFPFHRFYLYFHEKILGELI 209
           PD DPRSF QQA +HCAYC+G Y Q+GFP +ELQ+HNSWLFFPFHR+YLYF EKILG+LI
Sbjct: 165 PDDDPRSFKQQAAVHCAYCDGAYDQVGFPELELQIHNSWLFFPFHRYYLYFFEKILGKLI 224

Query: 210 GDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDE 269
            DPTFALPFWN+D+PAGM  PAI+A+  SPLYD+ R+ NH PPT+VDLDYNGT+ N+  E
Sbjct: 225 NDPTFALPFWNWDSPAGMPLPAIYADPKSPLYDKLRSANHQPPTLVDLDYNGTEDNVSKE 284

Query: 270 ARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPHIPIHRWCGNKHQP 329
             + +NL++MYRQMVS S+   LFFG PYRAGD+P PG GS+E  PH P+H W G+  QP
Sbjct: 285 TTINANLKIMYRQMVSNSKNAKLFFGNPYRAGDEPDPGGGSIEGTPHAPVHLWTGDNTQP 344

Query: 330 NGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNEKKEA 389
           N EDMGNFYSAGRDP+F++HH+NVDRMW IWKTLG +R D+ + D+L++ F+FYNE  E 
Sbjct: 345 NFEDMGNFYSAGRDPIFFAHHSNVDRMWSIWKTLGGKRTDLTDSDWLDSGFLFYNENAEL 404

Query: 390 VRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTG-AA 449
           VRV+VRDCL+T  LGYVYQDVDIPWL + P+PRR K+    VA          LG   AA
Sbjct: 405 VRVKVRDCLETKNLGYVYQDVDIPWLSSKPTPRRAKVALSKVAKK--------LGVAHAA 464

Query: 450 LAAESEL----EFPLSLESVIRTDVKRPK-KSRSSKEKEEEEEILVISGIEFDPNTAVKF 509
           +A+ S++    EFP+SL S I T VKRPK K RS K KE+EEEILVI GIEFD + AVKF
Sbjct: 465 VASSSKVVAGTEFPISLGSKISTVVKRPKQKKRSKKAKEDEEEILVIEGIEFDRDVAVKF 524

Query: 510 DVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIEDQDSVV 569
           DV +N  +D     D TEFAGSF SVPH + +   + T LRLG+T+LLE++  ED DSVV
Sbjct: 525 DVYVNDVDDLPSGPDKTEFAGSFVSVPHSHKHKKKMNTILRLGLTDLLEEIEAEDDDSVV 584

Query: 570 VTLVPRIKDQNITIGGIEIEF 582
           VTLVP  K   + IGGI+IEF
Sbjct: 585 VTLVP--KFGAVKIGGIKIEF 591

[Close]

BLAST of Moc03g27390 vs. ExPASy Swiss-Prot
Match: Q06215 (Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 2.2e-169
Identity = 305/587 (51.96%), Postives = 397/587 (67.63%), Query Frame = 0


Query: 11  SLLPSFRRKFVVNKNNKHCR-VVCSASNKHDS--QSQSPGNMV-DRRDVLIGLGGLYGAS 70
           S+ P  +++   +K  K  R V CS++N  ++  + Q   N+V  RR+VLIGLGG+YG  
Sbjct: 25  SVYPFLQKQHQSSKLRKPKRQVTCSSNNNQNNPKEEQELSNIVGHRRNVLIGLGGIYGTL 84

Query: 71  SFAPESDSFAIAAPVDTPQITKCG-----PAQVRDKKVECCPPPSIKIKDFRPTPPRKLR 130
           +  P     A+A+P+  P ++KC      P+      + CCPP S KI DF+    + LR
Sbjct: 85  ATNPS----ALASPISPPDLSKCVPPSDLPSGTTPPNINCCPPYSTKITDFKFPSNQPLR 144

Query: 131 VRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFP-VELQ 190
           VR AAHLVD  +L+KY++A ELM+ LP +DPR+F QQANIHCAYC+G Y Q+GFP ++LQ
Sbjct: 145 VRQAAHLVDNEFLEKYKKATELMKALPSNDPRNFTQQANIHCAYCDGAYSQIGFPDLKLQ 204

Query: 191 VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE 250
           VH SWLFFPFHR+YLYF+E+ILG LI DPTFALPFWNYDAP GM  P I+A++ SPLYDE
Sbjct: 205 VHGSWLFFPFHRWYLYFYERILGSLINDPTFALPFWNYDAPDGMQLPTIYADKASPLYDE 264

Query: 251 FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD 310
            RN +H PPT++DL++     ++D    +++NL +MYRQ+ S  +T  LF G PYRAGD 
Sbjct: 265 LRNASHQPPTLIDLNFCDIGSDIDRNELIKTNLSIMYRQVYSNGKTSRLFLGNPYRAGDA 324

Query: 311 PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL 370
              GAGS+ENVPH P+H W G+  Q N EDMG FYSA RDP+FYSHH+NVDR+W IWKTL
Sbjct: 325 EPQGAGSIENVPHAPVHTWTGDNTQTNIEDMGIFYSAARDPIFYSHHSNVDRLWYIWKTL 384

Query: 371 GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR 430
           G ++ D  + D+L + F+FY+E K  VRV V+D LD   LGY YQDV IPW K  P PRR
Sbjct: 385 GGKKHDFTDKDWLESGFLFYDENKNLVRVNVKDSLDIDKLGYAYQDVPIPWEKAKPVPRR 444

Query: 431 KKLRK---GGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKE 490
            K++K     V + N+ K P++       +    + FPL L + +   VKRPKK RS KE
Sbjct: 445 TKVQKLVEVEVNDGNLRKSPTIF-LVRQQSPRKYVTFPLVLNNKVSAIVKRPKKLRSKKE 504

Query: 491 KEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLK 550
           KEEEEE+LVI GIEF  N A+KFDV IN E+D+ + A NTEFAGSF ++PH      N K
Sbjct: 505 KEEEEEVLVIEGIEFYMNIAIKFDVYINDEDDK-VGAGNTEFAGSFVNIPHSAHGHKNKK 564

Query: 551 --TCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD 583
             T LRLGIT+LLEDL +E  D++VVTLVP+     + I  +EI F+
Sbjct: 565 IITSLRLGITDLLEDLHVEGDDNIVVTLVPKCGSGQVKINNVEIVFE 605

[Close]

BLAST of Moc03g27390 vs. ExPASy Swiss-Prot
Match: P43311 (Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 2.3e-166
Identity = 308/597 (51.59%), Postives = 398/597 (66.67%), Query Frame = 0


Query: 9   PPSLLPSFRRKFVV----NKNNKHC--RVVCSASN---KHDSQS---QSPGNMVDRRDVL 68
           PPS  P F+R   V    N++ +    +V C+++N     DS S   ++    +DRR+VL
Sbjct: 24  PPS--PLFQRASHVPVARNRSRRFAPSKVSCNSANGDPNSDSTSDVRETSSGKLDRRNVL 83

Query: 69  IGLGGLYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFR- 128
           +G+GGLYGA+     +   A  AP+  P I+KCG A V D      CCPP + KI DF+ 
Sbjct: 84  LGIGGLYGAAGGLGATKPLAFGAPIQAPDISKCGTATVPDGVTPTNCCPPVTTKIIDFQL 143

Query: 129 PTPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQL 188
           P+    +R RPAAHLV K YL KY++A+EL + LPD DPRSF QQAN+HC YC G Y Q+
Sbjct: 144 PSSGSPMRTRPAAHLVSKEYLAKYKKAIELQKALPDDDPRSFKQQANVHCTYCQGAYDQV 203

Query: 189 GF-PVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFAN 248
           G+  +ELQVH SWLF PFHR+YLYF+E+IL +LI DPTFALP+W +D P GM  P I+A+
Sbjct: 204 GYTDLELQVHASWLFLPFHRYYLYFNERILAKLIDDPTFALPYWAWDNPDGMYMPTIYAS 263

Query: 249 QNSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFG 308
             S LYDE RN  H+PPTV+DLDY+GT+  + D+     NL +MY+Q+VSG+ T  LF G
Sbjct: 264 SPSSLYDEKRNAKHLPPTVIDLDYDGTEPTIPDDELKTDNLAIMYKQIVSGATTPKLFLG 323

Query: 309 RPYRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDR 368
            PYRAGD   PGAG++E+ PH  +H+W G   +P+ EDMGNFY+AGRDP+F+ HHANVDR
Sbjct: 324 YPYRAGDAIDPGAGTLEHAPHNIVHKWTGLADKPS-EDMGNFYTAGRDPIFFGHHANVDR 383

Query: 369 MWCIWKTL-GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPW 428
           MW IWKT+ G+ R+D  + D+L+A+FVFY+E K+ V+V+V DC+DT+ L Y YQD+ IPW
Sbjct: 384 MWNIWKTIGGKNRKDFTDTDWLDATFVFYDENKQLVKVKVSDCVDTSKLRYQYQDIPIPW 443

Query: 429 LKTAPSPRRK---KLRKGGVAN-NNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVK 488
           L      + K   K  K GVA    +PK              S  +FP +L SVIR +V 
Sbjct: 444 LPKNTKAKAKTTTKSSKSGVAKAAELPK----------TTISSIGDFPKALNSVIRVEVP 503

Query: 489 RPKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVP 548
           RPKKSRS KEKE+EEE+L+I GIE D    VKFDV IN E+    R  N+EFAGSF +VP
Sbjct: 504 RPKKSRSKKEKEDEEEVLLIKGIELDRENFVKFDVYINDEDYSVSRPKNSEFAGSFVNVP 563

Query: 549 HQNANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDQD 585
           H++      KT LR  I ELLEDLG ED +SV+VT+VPR    ++TIGGIEIEF  D
Sbjct: 564 HKHMKEMKTKTNLRFAINELLEDLGAEDDESVIVTIVPRAGGDDVTIGGIEIEFVSD 607

[Close]

BLAST of Moc03g27390 vs. ExPASy Swiss-Prot
Match: Q9MB14 (Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=2)

HSP 1 Score: 561.2 bits (1445), Expect = 1.4e-158
Identity = 291/593 (49.07%), Postives = 393/593 (66.27%), Query Frame = 0


Query: 2   ASVCPPLP-PSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG 61
           A++  PLP PS +          K   H +V C+A N  DSQ +     +DRRDVL+GLG
Sbjct: 24  ATISSPLPKPSQI-----HIATAKRTHHFKVSCNAPN-GDSQPK-----LDRRDVLLGLG 83

Query: 62  GLYGASSFAPESDSFAIAAPVDTPQITKC--GPAQVRDKKV--ECCPPPSIKIKDFR--P 121
           GL GA+S    ++  A A P+  P+I+KC   P  +    +   CCPP +  +  ++   
Sbjct: 84  GLAGAASLI--NNPLAFAEPIHAPEISKCVVPPKDLPPDAIVDNCCPPLATNVIPYKVPK 143

Query: 122 TPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLG 181
           T P  +++RPA H +DK Y+ K+E+A+ LM+ LP  DPR+F QQA +HCAYCNGGY Q  
Sbjct: 144 TSPSAMKIRPAIHRMDKEYIAKFEKAIRLMKELPADDPRNFYQQALVHCAYCNGGYVQTD 203

Query: 182 FP-VELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQ 241
           +P  E+QVHNSWLFFPFHR+YLYF+E+ILG+LIGDPTF LPFWN+D PAGM  P  F NQ
Sbjct: 204 YPDKEIQVHNSWLFFPFHRWYLYFYERILGKLIGDPTFGLPFWNWDTPAGMLIPQYFRNQ 263

Query: 242 NSPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGR 301
           NSPLYDE RN++H+ P V+DL Y GTD ++ D+ R+ +NL +MY+ MV+ + T  LF G+
Sbjct: 264 NSPLYDENRNQSHL-PLVMDLGYAGTDTDVTDQERISNNLALMYKSMVTNAGTAELFLGK 323

Query: 302 PYRAGDDP-SPGAGSVENVPHIPIHRWCGN--KHQPNGEDMGNFYSAGRDPVFYSHHANV 361
           PY+AGDDP + G GS+EN+PH P+HRW G+      NGEDMGNFYSAGRD +FY HH+NV
Sbjct: 324 PYKAGDDPVNKGGGSIENIPHTPVHRWVGDVKPRTQNGEDMGNFYSAGRDILFYCHHSNV 383

Query: 362 DRMWCIWKTLG--RRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVD 421
           DRMW IW+ LG   RRRD  + D+L+A+F+FY+E K+AVRVRV D LD   LGY Y+  +
Sbjct: 384 DRMWTIWQQLGGKGRRRDFTDSDWLDATFIFYDENKQAVRVRVGDALDNQKLGYKYEFTN 443

Query: 422 IPWLKTAPSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKR 481
           +PWL + P P +KK    G+A  +   F + +             FPL+L+ V++  V R
Sbjct: 444 LPWLNSKPLPTKKKT---GLAARSKAPFVTDV-------------FPLTLDKVVQVKVPR 503

Query: 482 PKKSRSSKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPH 541
           PKKSRS +EKE EEEIL I GIE   +   KFDV +N E++ E   +  E+AGSFA +PH
Sbjct: 504 PKKSRSKEEKEAEEEILEIQGIEVAIDQYAKFDVYLNDEDEPEAGKEKAEYAGSFAHLPH 563

Query: 542 QNANMNNLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 582
           ++     ++T L LG+ E LEDLG ED D+V+VTL P++    +T+  I+I +
Sbjct: 564 KHTGSKKIRTSLSLGLNEPLEDLGAEDDDAVLVTLAPKVGGGVVTVENIKIVY 586

[Close]

BLAST of Moc03g27390 vs. ExPASy Swiss-Prot
Match: O81103 (Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PPO PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 285/566 (50.35%), Postives = 374/566 (66.08%), Query Frame = 0


Query: 31  VVCSASNKHDSQSQSPG--------NMVDRRDVLIGLGGLYGASSFAPESDSFAIAAPVD 90
           + C A+N ++S +Q+            +DRR++LIGLGGLYGA++   +   FA A P+ 
Sbjct: 48  ISCKATNNNNSNNQNEQQEESSRLLGKLDRRNILIGLGGLYGATTL--DRKPFAFADPIA 107

Query: 91  TPQITKCGPAQVR---DKKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYER 150
            P +T C PA++     + V CCPP + KIK F+P     LR  PAAH V   YL K+++
Sbjct: 108 PPDLTTCKPAEITPGGSETVPCCPPVTTKIKTFKPDLSIPLRTSPAAHQVTDEYLAKFKK 167

Query: 151 AVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGF-PVELQVHNSWLFFPFHRFYLYFH 210
           A   MR LPD DPRS +QQA +HCAYCNG Y Q+GF   ++QVH SWLFFPFHR YLYF+
Sbjct: 168 AQAAMRALPDDDPRSMVQQAKVHCAYCNGAYPQVGFTDNDIQVHFSWLFFPFHRMYLYFY 227

Query: 211 EKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNG 270
           E+ILG+LI DPTFALP+WN+D+P G   P I+ + +SPLYD++RN +H PP +VDL Y G
Sbjct: 228 ERILGKLIDDPTFALPYWNWDSPVGFPIPDIYTDTSSPLYDQYRNADHQPPVLVDLSYGG 287

Query: 271 TDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD-DPSPGAGSVENVPHIPIH 330
            D ++D++ R+  NL +MYRQMVSG++T  LFFG  YRAG+ +     G++EN+PH  IH
Sbjct: 288 KDDDVDEQTRIDENLAIMYRQMVSGAKTPDLFFGHAYRAGNLNTGKYPGTIENMPHNNIH 347

Query: 331 RWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASF 390
            W G+  Q + EDMGNFYSAGRDP+FY+HHANVDRMW IWKTLG +R+DI + D+L+A F
Sbjct: 348 IWVGDPSQTHQEDMGNFYSAGRDPLFYAHHANVDRMWNIWKTLGGKRKDITDTDWLDAEF 407

Query: 391 VFYNEKKEAVRVRVRDCLD-TTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKF 450
           +FY+E  E VRV+VRD L+    L Y Y+ V +PWL T P+ R+ K       N    K 
Sbjct: 408 LFYDENAELVRVKVRDSLEPEKQLRYNYEPVSLPWLFTKPTARKTK-------NKTKAK- 467

Query: 451 PSLLGTGAALAAESELEFPLSLESVIRTDVKRPK-KSRSSKEKEEEEEILVISGIEFDPN 510
                     A +   +FP +L  V   +V RPK + RS KEK +EEE+L+I  IEF+  
Sbjct: 468 --------VAATQLTSKFPATLVEVTTVEVARPKPRKRSKKEKVDEEELLIIKDIEFEGT 527

Query: 511 TAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIED 570
            AVKFDV IN + +   R D +EFAGSF  VP         KT L+LGIT+LLEDLG ED
Sbjct: 528 EAVKFDVFINDDAESLSRRDKSEFAGSFVHVPQGKTTKAKTKTNLKLGITDLLEDLGAED 587

Query: 571 QDSVVVTLVPRIKDQNITIGGIEIEF 582
             SV+VTLVPR+ +  ITIGG +IE+
Sbjct: 588 DSSVLVTLVPRVSNSPITIGGFKIEY 595

[Close]

BLAST of Moc03g27390 vs. ExPASy TrEMBL
Match: A0A6J1CGK8 (polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC111010634 PE=3 SV=1)

HSP 1 Score: 1207.6 bits (3123), Expect = 0.0e+00
Identity = 584/586 (99.66%), Postives = 584/586 (99.66%), Query Frame = 0


Query: 1   MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG 60
           MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG
Sbjct: 9   MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG 68

Query: 61  GLYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKL 120
           GLYGASSFAPE DSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKL
Sbjct: 69  GLYGASSFAPEXDSFAIAAPVDTPQITKCGPAQVRDKKVECCPPPSIKIKDFRPTPPRKL 128

Query: 121 RVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQ 180
           RVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQ
Sbjct: 129 RVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQ 188

Query: 181 VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE 240
           VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE
Sbjct: 189 VHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDE 248

Query: 241 FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD 300
           FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD
Sbjct: 249 FRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDD 308

Query: 301 PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL 360
           PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL
Sbjct: 309 PSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTL 368

Query: 361 GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR 420
           GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR
Sbjct: 369 GRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRR 428

Query: 421 KKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEE 480
           KKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEE
Sbjct: 429 KKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEE 488

Query: 481 EEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCL 540
           EEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCL
Sbjct: 489 EEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCL 548

Query: 541 RLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDQDDD 587
           RLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFD DDD
Sbjct: 549 RLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDPDDD 594

[Close]

BLAST of Moc03g27390 vs. ExPASy TrEMBL
Match: A0A6J1KRC0 (polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111495765 PE=3 SV=1)

HSP 1 Score: 742.7 bits (1916), Expect = 1.2e-210
Identity = 358/587 (60.99%), Postives = 449/587 (76.49%), Query Frame = 0


Query: 1   MASVCPPL--PPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIG 60
           MAS+ PP     +  PS R        N   RV+CSA+N            +DRRDVLIG
Sbjct: 1   MASLPPPALSTATFRPSSRPPTSKYLPNNAARVLCSAAN----------GKIDRRDVLIG 60

Query: 61  L--GGLYGASSFAPESDSFAIAAPVDTPQITKCGPAQV--RDKKVECCPPPSIKIKDFRP 120
           +  GG+YGAS+F  +S +FA+AAPV TP I+KCGP  +        CCPPPS  I DFRP
Sbjct: 61  IGAGGIYGASAFTKDS-TFALAAPVQTPNISKCGPPDLPPGSDPTNCCPPPSPSIVDFRP 120

Query: 121 TPPRKLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLG 180
             P KLRVRPAAHL D++Y+ KY+RAVELM+GLP  DPRSFMQQAN+HCAYCNGGY Q+G
Sbjct: 121 PAPGKLRVRPAAHLADQSYIDKYKRAVELMKGLPGDDPRSFMQQANVHCAYCNGGYDQVG 180

Query: 181 FPVELQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQN 240
           FPVE+QVHNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+ N
Sbjct: 181 FPVEVQVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMRMPAMYADHN 240

Query: 241 SPLYDEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRP 300
           S LYD+ RN+NH+PPT++DLD++G D N+ D A++RSNLR+MYRQMVS SRT  LFFG  
Sbjct: 241 SSLYDQLRNQNHLPPTLMDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSA 300

Query: 301 YRAGDDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMW 360
           Y AGD+PSPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+NVDRMW
Sbjct: 301 YVAGDEPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNVDRMW 360

Query: 361 CIWKTLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKT 420
            +WKTLG RRRD+Q P++L+ASF+FYNEK +AVRVRVRDCLDT  LGY+YQDV++PWL+T
Sbjct: 361 SVWKTLGGRRRDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELPWLET 420

Query: 421 APSPRRKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRS 480
            P+PRR  LR     ++N+P         +      +++ P++L+S I T+VKR KKSR 
Sbjct: 421 RPTPRR-ALRAQKSVDSNVP---------SRRVKPPKMKLPVTLKSAISTEVKRGKKSRG 480

Query: 481 SKEKEEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMN 540
            KEK+EEEEILVI GIEFDPNT++K D+ IN E+DREIR DNTEFAGSF +VPH++ +  
Sbjct: 481 RKEKDEEEEILVIKGIEFDPNTSIKLDIYINDEDDREIRGDNTEFAGSFVNVPHKHGHKK 540

Query: 541 NLKTCLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 582
            +KT LRLGI+E+LEDL  +D DS++VT++PR+    ++I  I+IE+
Sbjct: 541 KVKTGLRLGISEVLEDLDADDDDSIIVTIIPRLGAGLVSIAEIKIEY 566

[Close]

BLAST of Moc03g27390 vs. ExPASy TrEMBL
Match: A0A1L5JHU3 (Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 740.0 bits (1909), Expect = 7.9e-210
Identity = 352/565 (62.30%), Postives = 439/565 (77.70%), Query Frame = 0


Query: 25  NNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLGGLYGASSFAPESDSFAIAAPVDTP 84
           ++K  R++CSASN          N +DRRD+LIGLGGLYGAS+ +    S A AAPV TP
Sbjct: 38  SSKAPRILCSASN-------GGQNHIDRRDILIGLGGLYGASTLS--QGSLAFAAPVQTP 97

Query: 85  QITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRKLRVRPAAHLVDKNYLKKYERAVE 144
            I+KCG   +      + CCPPP+  + DFRP  P KLRVRPAAHL D+NY++KY+RAVE
Sbjct: 98  NISKCGQPDLPPGADPINCCPPPTTAVVDFRPQAPGKLRVRPAAHLADQNYVEKYKRAVE 157

Query: 145 LMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVELQVHNSWLFFPFHRFYLYFHEKIL 204
           LM+ LPD DPRSF QQAN+HCAYCNG Y Q+GFPVE+QVHNSWLFFPFHR+YL+FHEKIL
Sbjct: 158 LMKALPDDDPRSFKQQANVHCAYCNGAYDQVGFPVEVQVHNSWLFFPFHRYYLHFHEKIL 217

Query: 205 GELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYDEFRNENHMPPTVVDLDYNGTDVN 264
           GELIGDP+FALPFWNYDAP GM  PA++A++ S LYDE RN NH+PPT+VDLD+ G D  
Sbjct: 218 GELIGDPSFALPFWNYDAPGGMKMPAMYADEKSSLYDELRNRNHLPPTLVDLDFGGVDPT 277

Query: 265 MDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGDDPSPGAGSVENVPHIPIHRWCGN 324
           + DEA++RSNL +MYRQMVS SRT SLFFG  Y AGD+PSPG G VEN+PH P+H WCG+
Sbjct: 278 IGDEAQIRSNLSIMYRQMVSNSRTPSLFFGNAYLAGDEPSPGGGLVENIPHGPVHIWCGD 337

Query: 325 KHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKTLGRRRRDIQNPDFLNASFVFYNE 384
            ++PN E+MGNFYSA RDP+FY+HH+N+DR+W +WKTLG RR+D+QN D+LNASF+FY+E
Sbjct: 338 NNEPNFENMGNFYSAARDPIFYAHHSNIDRLWSVWKTLGGRRQDLQNADWLNASFIFYDE 397

Query: 385 KKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPRRKKLRKGGVANNNMPKFPSLL-- 444
           K +AVRVRVRDCLD+  LGYVYQDV +PWL++ P+PRR    +  VA+      P+L   
Sbjct: 398 KAQAVRVRVRDCLDSRNLGYVYQDVQLPWLESRPTPRRALRAQKAVAHLPFGVGPALAAG 457

Query: 445 --GTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKEEEEEILVISGIEFDPNTAV 504
             G  ++    ++++FP++L S I  +VKRPK+SRS KEK+EEEEI+VI GIEFD N A+
Sbjct: 458 EPGPSSSRLRPAKIKFPITLNSPISMEVKRPKRSRSKKEKDEEEEIIVIYGIEFDANKAI 517

Query: 505 KFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTCLRLGITELLEDLGIEDQDS 564
           KFDV IN E+D+EIR DNTEFAGSF +VPH++ +  N+KT LRLGITELLEDL  +D DS
Sbjct: 518 KFDVFINDEDDKEIRPDNTEFAGSFVNVPHKHGHKKNVKTGLRLGITELLEDLDADDDDS 577

Query: 565 VVVTLVPRIKDQNITIGGIEIEFDQ 584
           V VTLVPR     ++I GI+IEFD+
Sbjct: 578 VRVTLVPRSGKGLVSIAGIKIEFDK 593

[Close]

BLAST of Moc03g27390 vs. ExPASy TrEMBL
Match: A0A0A0LXD0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 5.6e-208
Identity = 344/585 (58.80%), Postives = 450/585 (76.92%), Query Frame = 0


Query: 1   MASVCPPLPPSLLPSFRRKFVVNKNNKHCRVVCSASNKHDSQSQSPGNMVDRRDVLIGLG 60
           MAS+  P   +L P  R+   +       R+ C A+N +D    +P   +DRRD+LIGL 
Sbjct: 1   MASLSHPPSLTLTPFLRKTKTLLITP---RISCKATNHNDQNFPNP--KIDRRDILIGLT 60

Query: 61  GLYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPR 120
           GLYG+S+ + +S  FA+AAP++TP I+KCGP  +        CCPPP+  I DF+P PP 
Sbjct: 61  GLYGSSTLS-QSTHFALAAPIETPNISKCGPPDLPPGADATNCCPPPATTILDFQPPPPS 120

Query: 121 KLRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVE 180
           KLRVRPAAHL D+NY++KY++AVELM+ LP+ DPRSF QQAN+HCAYCNG Y Q+GFPVE
Sbjct: 121 KLRVRPAAHLADRNYIEKYKKAVELMKALPEDDPRSFTQQANVHCAYCNGAYDQVGFPVE 180

Query: 181 LQVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLY 240
           +QVHNSWLFFPFHRFYLYFHEKILGELIGDP+FALPFWNYDAP GM  P ++A + S LY
Sbjct: 181 VQVHNSWLFFPFHRFYLYFHEKILGELIGDPSFALPFWNYDAPDGMKMPGMYAEEKSSLY 240

Query: 241 DEFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAG 300
           DE RNENH+PP +VDLD+ G D N+ D+A+++SNL +MYRQMVSGSRT SLFFG  Y AG
Sbjct: 241 DELRNENHLPPRLVDLDFGGVDPNIGDDAQIKSNLSIMYRQMVSGSRTPSLFFGSAYVAG 300

Query: 301 DDPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWK 360
           D+PSPG GSVEN+PH P+H WCG+  QPN E+MGNFYSA RDP+F++HH+N+DR+W +WK
Sbjct: 301 DEPSPGGGSVENIPHGPVHVWCGDTDQPNFENMGNFYSAARDPIFFAHHSNIDRLWSVWK 360

Query: 361 TLGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSP 420
           TLG RR+++QNP++LNASF+FY+EK + +RVRVRDCLD+ +LGY+YQ VD+PWL+T P+P
Sbjct: 361 TLGGRRQNLQNPEWLNASFIFYDEKAQPIRVRVRDCLDSKSLGYIYQPVDLPWLQTRPTP 420

Query: 421 RRKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEK 480
           RR + +   VA    P   +  G  ++    + ++FP++++S +  +VKRPKKSRS KEK
Sbjct: 421 RRAQ-KTSTVAFGIAPAL-AAGGPSSSRVKPANIKFPITVKSPVSVEVKRPKKSRSKKEK 480

Query: 481 EEEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKT 540
           EEEEE+LVI GI FDPN  +KFDV IN E+D+EI+ DNTEFAGSF +VPH++ +   +KT
Sbjct: 481 EEEEEVLVIYGIGFDPNNGIKFDVFINDEDDKEIKPDNTEFAGSFVNVPHKHGHKKKVKT 540

Query: 541 CLRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEFDQ 584
            LR+GITE LEDL  ++ DS++VT++PRI     +I G++IEFD+
Sbjct: 541 GLRVGITEELEDLEADNDDSIIVTILPRIGAGLFSIDGLKIEFDK 577

[Close]

BLAST of Moc03g27390 vs. ExPASy TrEMBL
Match: A0A6J1EGP5 (polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111434015 PE=3 SV=1)

HSP 1 Score: 733.8 bits (1893), Expect = 5.6e-208
Identity = 351/582 (60.31%), Postives = 447/582 (76.80%), Query Frame = 0


Query: 9   PPSLLPSFRRKFVVNKNNKH-----CRVVCSASNKHDSQSQSPGNMVDRRDVLIGL--GG 68
           PP+L  +  R     + +K+      R++CSA++            +DRRDVLIG+  GG
Sbjct: 6   PPALSTAAFRPSSSLRTSKYLPINAARILCSAAD----------GKIDRRDVLIGIGAGG 65

Query: 69  LYGASSFAPESDSFAIAAPVDTPQITKCGPAQVRD--KKVECCPPPSIKIKDFRPTPPRK 128
           LYGASSF  +S +FA+AAPV TP I+KCGP  +        CCPPP+  I DF P  P K
Sbjct: 66  LYGASSFTKDS-TFALAAPVQTPNISKCGPPDLPPGADPTNCCPPPTTSIIDFLPPAPGK 125

Query: 129 LRVRPAAHLVDKNYLKKYERAVELMRGLPDHDPRSFMQQANIHCAYCNGGYKQLGFPVEL 188
           LRVRPAAHL D++Y+ KY+RAVELM+GLP+ DPRSFMQQAN+HCAYCNGGY Q+GFPVE+
Sbjct: 126 LRVRPAAHLADQSYIDKYKRAVELMKGLPEDDPRSFMQQANVHCAYCNGGYDQVGFPVEV 185

Query: 189 QVHNSWLFFPFHRFYLYFHEKILGELIGDPTFALPFWNYDAPAGMTFPAIFANQNSPLYD 248
           QVHNSWLFFPFHR+YLYF+EKILGELIGDPTFALPFWNYDAP GM  PA++A+QNS LYD
Sbjct: 186 QVHNSWLFFPFHRYYLYFYEKILGELIGDPTFALPFWNYDAPGGMKMPAMYADQNSSLYD 245

Query: 249 EFRNENHMPPTVVDLDYNGTDVNMDDEARVRSNLRVMYRQMVSGSRTRSLFFGRPYRAGD 308
           + RN+NH+PPT +DLD++G D N+ D A++RSNLR+MYRQMVS SRT  LFFG  Y AGD
Sbjct: 246 QLRNQNHLPPTFIDLDFDGVDSNIGDNAQIRSNLRIMYRQMVSNSRTPKLFFGSAYVAGD 305

Query: 309 DPSPGAGSVENVPHIPIHRWCGNKHQPNGEDMGNFYSAGRDPVFYSHHANVDRMWCIWKT 368
           +PSPG GSVEN+PH  +H WCG+ ++PN E+MGNFYSA RDP+FYSHH+N+DRMW +WKT
Sbjct: 306 EPSPGGGSVENIPHNTVHTWCGDNNEPNLENMGNFYSAARDPIFYSHHSNIDRMWSVWKT 365

Query: 369 LGRRRRDIQNPDFLNASFVFYNEKKEAVRVRVRDCLDTTALGYVYQDVDIPWLKTAPSPR 428
           LG RR+D+Q P++L+ASF+FYNEK +AVRVRVRDCLDT  LGY+YQDV++ WL+T P+PR
Sbjct: 366 LGGRRQDLQYPEWLDASFIFYNEKAQAVRVRVRDCLDTKNLGYIYQDVELLWLETRPTPR 425

Query: 429 RKKLRKGGVANNNMPKFPSLLGTGAALAAESELEFPLSLESVIRTDVKRPKKSRSSKEKE 488
           R  LR     ++N+P         +     + ++FP++++S + T+VKR KKSRS KEKE
Sbjct: 426 R-ALRAQKSVDSNVP---------SRRVKPARIKFPVTVKSAVSTEVKRAKKSRSKKEKE 485

Query: 489 EEEEILVISGIEFDPNTAVKFDVLINSEEDREIRADNTEFAGSFASVPHQNANMNNLKTC 548
           EEEEILVI GIEFDPNTA+K DV IN E+D EIR DNTEFAGSF +VPH++ +   +KT 
Sbjct: 486 EEEEILVIKGIEFDPNTAIKLDVYINDEDDSEIRGDNTEFAGSFVNVPHKHGHKKKVKTG 545

Query: 549 LRLGITELLEDLGIEDQDSVVVTLVPRIKDQNITIGGIEIEF 582
           LRLGI+E+LEDL  +D DS++VT++PR+    ++I  I +E+
Sbjct: 546 LRLGISEVLEDLEADDDDSIIVTIIPRLGAGLVSIAEITMEY 566

The following BLAST results are available for this feature:

BLAST of Moc03g27390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139808.1	0.0e+00	99.66	polyphenol oxidase, chloroplastic-like [Momordica charantia]	[more]
XP_023001713.1	2.5e-210	60.99	polyphenol oxidase, chloroplastic-like [Cucurbita maxima]	[more]
XP_038903169.1	1.2e-209	60.68	polyphenol oxidase, chloroplastic-like [Benincasa hispida]	[more]
APO14262.1	1.6e-209	62.30	polyphenol oxidase 3 [Luffa aegyptiaca]	[more]
XP_023519283.1	4.0e-208	60.14	polyphenol oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc03g27390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43309	1.3e-190	58.82	Polyphenol oxidase, chloroplastic OS=Malus domestica OX=3750 PE=1 SV=1	[more]
Q06215	2.2e-169	51.96	Polyphenol oxidase A1, chloroplastic OS=Vicia faba OX=3906 PE=1 SV=1	[more]
P43311	2.3e-166	51.59	Polyphenol oxidase, chloroplastic OS=Vitis vinifera OX=29760 PE=1 SV=1	[more]
Q9MB14	1.4e-158	49.07	Polyphenol oxidase II, chloroplastic OS=Ipomoea batatas OX=4120 GN=co-2 PE=2 SV=...	[more]
O81103	1.1e-157	50.35	Polyphenol oxidase latent form, chloroplastic OS=Prunus armeniaca OX=36596 GN=PP...	[more]

BLAST of Moc03g27390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CGK8	0.0e+00	99.66	polyphenol oxidase, chloroplastic-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1KRC0	1.2e-210	60.99	polyphenol oxidase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114957...	[more]
A0A1L5JHU3	7.9e-210	62.30	Polyphenol oxidase 3 OS=Luffa aegyptiaca OX=3670 PE=2 SV=1	[more]
A0A0A0LXD0	5.6e-208	58.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G073640 PE=3 SV=1	[more]
A0A6J1EGP5	5.6e-208	60.31	polyphenol oxidase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]

BLAST of Moc03g27390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002227	Tyrosinase copper-binding domain	PRINTS	PR00092	TYROSINASE	coord: 182..199 score: 56.35 coord: 213..218 score: 55.1 coord: 339..357 score: 56.18 coord: 312..323 score: 32.99
IPR002227	Tyrosinase copper-binding domain	PFAM	PF00264	Tyrosinase	coord: 152..359 e-value: 7.7E-33 score: 114.6
IPR002227	Tyrosinase copper-binding domain	PROSITE	PS00497	TYROSINASE_1	coord: 182..199
IPR002227	Tyrosinase copper-binding domain	PROSITE	PS00498	TYROSINASE_2	coord: 340..351
IPR016213	Polyphenol oxidase	PIRSF	PIRSF000290	PPO_plant	coord: 7..583 e-value: 8.5E-251 score: 831.8
IPR022740	Polyphenol oxidase, C-terminal	PFAM	PF12143	PPO1_KFDV	coord: 454..581 e-value: 9.9E-50 score: 168.0
IPR008922	Uncharacterised domain, di-copper centre	GENE3D	1.10.1280.10		coord: 79..413 e-value: 3.6E-119 score: 400.1
IPR008922	Uncharacterised domain, di-copper centre	SUPERFAMILY	48056	Di-copper centre-containing domain	coord: 95..412
IPR022739	Polyphenol oxidase, central domain	PFAM	PF12142	PPO1_DWL	coord: 366..416 e-value: 1.9E-22 score: 78.7
None	No IPR available	PIRSR	PIRSR000290-1	PIRSR000290-1	coord: 79..582 e-value: 5.3E-226 score: 749.1
None	No IPR available	PANTHER	PTHR11474	TYROSINASE FAMILY MEMBER	coord: 1..582
None	No IPR available	PANTHER	PTHR11474:SF94	POLYPHENOL OXIDASE, CHLOROPLASTIC-LIKE	coord: 1..582

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g27390.1	Moc03g27390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046148	pigment biosynthetic process
molecular_function	GO:0004097	catechol oxidase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0016491	oxidoreductase activity