Moc03g27060 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g27060
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionProtein SMG7L
Locationchr3: 19489615 .. 19491366 (-)
RNA-Seq ExpressionMoc03g27060
SyntenyMoc03g27060
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGACGCCTAATTGAGAGATGTTTGAAGGCAACCCAATTGGTTTCTTTCCCTCTTTTACCCGCTGTGCTCGTCTTTGTGGAGTGGTTAGCGAACGTTCTTGATGGAGTATCAAAATATGGTTCTGATGAAAAGAGCAGAAGTTCCATGTCTTACTTTTTTGGTGTTTTTGTTAACCTTCTAGAGAGACTGAATGTTAATACAGTTGACGCAGAGTCTTCACTTGCTATCCCACTGTGGGAAGACTATGAATTAAGGGGCTTCACACCTTTGGCTTCTGCACATGAACCATTAGATTTCTCATCTCACTGGGAACACATGGACAACTACAAATTTGGAGGAAAACATCGTGCTTACCGCATAATTGTTGCTGCTACCAAAATTTCCAACACAGCTAATGATTCTCCAAAGTGTATCATCCACGATAAGACGCGGAAGGTCTTTTACATAGTAGAACAAAATGAACTTGCAGACAAGAAAGCATTGGAAAGTGCAAAGTCGAACATTGTTAGTCCAGATCCGCAAGTGCCAACTCGGGACGTCGGTGAAGATATCCCAGATGAAGTTCAGGATCAGAACCATCTGAATAAGAAATTTGTCACGGTTGAAGATGAAGAAGTCATTCTTTTCAAGCCCCTTATGAGGTATAATTCTGCACCGATCTCTATTGCAGGGAACGGTGAAATTTCACCAAAAAGCGTGGAGGATCAGACCGTTTCTTCTGATGAATGCTTGAGGCGTGCTACATCGCTACTTATAGCACAGACTCAGGGCCAGAGTGATCCCTTTGCTTTTCAAACAGACATTACAAATGTCAACTCAAACAAGTTATCTGAGCAGCATGATACTATGGTAAAAGATACAAGAGAACATCAAATGTCAGAAGGCTCCATATCTGCTGGCCCCCCCTCTCTCAGTGCCTGGGTGCTCAATAGAGGAGGTTTTACTTTGAACCCTGATAGAGAGAAAGGGACAAATGGTTTTGCTAAACCTGGTTTACAGCCGATTGACGAGTTAACTCCGACGTTTATAAATGGTTCTAGACTTGGTGATACCGAGAATTCTGCTTCGAGTCCCAGCCGTGAATCTGGAAAATCATACCAGTTTCCTCCTCCTCCCTATTCAGCCCCAACACCTTCAGCTCCTTATTTACCTGATGATGCCGTTTGGTTTAATGGTACCAATGCTGGCATGTCCGAAAGCAAAATCACTAGGGACATCGACCAAAATGGTACATTCTCAAATGCTTTTAGAGGAAGTCCAAATTGGCCTGCCACTCATGGGACACATGCATATGGCCCCTTGACTGCTGGCCTTCCGAATATACAACCGTTCACGCATCGAATGACTTCTTCTGAATGGCTTCGTCAGTATCGGGAGAATCACAATCTGGAGCGGGATAGTAGTCAGTTAATGCCAGCTCCCTACAATGCTTCAGGAAATCTTATGAACTTCCAAAGAAATGATGCTTCAAGGAATGACTATTTGTATCAAACAGGGAGCCAATTGGGTTATAATCAAACAATGAATATGGAGAGTCCATTGCGCCATCCAGCTTTCCCATTAGCTTATGGAACTAATGAAAACCAGAAAAACATGATCTTCCATGGTTACGAAAGGCCGAACCTTTATGGCTGCGGTGCTACTGACTTGAGAAGTGAGCAGCCGCCGCTTCTGCTGTATCTAAAAGAGAAAGAATGGCAGCTCCAGAAAGATGCTGCTAGCAGAACTCCCACCTATATGGGGAATTGA

mRNA sequence

ATGGGACGCCTAATTGAGAGATGTTTGAAGGCAACCCAATTGGTTTCTTTCCCTCTTTTACCCGCTGTGCTCGTCTTTGTGGAGTGGTTAGCGAACGTTCTTGATGGAGTATCAAAATATGGTTCTGATGAAAAGAGCAGAAGTTCCATGTCTTACTTTTTTGGTGTTTTTGTTAACCTTCTAGAGAGACTGAATGTTAATACAGTTGACGCAGAGTCTTCACTTGCTATCCCACTGTGGGAAGACTATGAATTAAGGGGCTTCACACCTTTGGCTTCTGCACATGAACCATTAGATTTCTCATCTCACTGGGAACACATGGACAACTACAAATTTGGAGGAAAACATCGTGCTTACCGCATAATTGTTGCTGCTACCAAAATTTCCAACACAGCTAATGATTCTCCAAAGTGTATCATCCACGATAAGACGCGGAAGGTCTTTTACATAGTAGAACAAAATGAACTTGCAGACAAGAAAGCATTGGAAAGTGCAAAGTCGAACATTGTTAGTCCAGATCCGCAAGTGCCAACTCGGGACGTCGGTGAAGATATCCCAGATGAAGTTCAGGATCAGAACCATCTGAATAAGAAATTTGTCACGGTTGAAGATGAAGAAGTCATTCTTTTCAAGCCCCTTATGAGGTATAATTCTGCACCGATCTCTATTGCAGGGAACGGTGAAATTTCACCAAAAAGCGTGGAGGATCAGACCGTTTCTTCTGATGAATGCTTGAGGCGTGCTACATCGCTACTTATAGCACAGACTCAGGGCCAGAGTGATCCCTTTGCTTTTCAAACAGACATTACAAATGTCAACTCAAACAAGTTATCTGAGCAGCATGATACTATGGTAAAAGATACAAGAGAACATCAAATGTCAGAAGGCTCCATATCTGCTGGCCCCCCCTCTCTCAGTGCCTGGGTGCTCAATAGAGGAGGTTTTACTTTGAACCCTGATAGAGAGAAAGGGACAAATGGTTTTGCTAAACCTGGTTTACAGCCGATTGACGAGTTAACTCCGACGTTTATAAATGGTTCTAGACTTGGTGATACCGAGAATTCTGCTTCGAGTCCCAGCCGTGAATCTGGAAAATCATACCAGTTTCCTCCTCCTCCCTATTCAGCCCCAACACCTTCAGCTCCTTATTTACCTGATGATGCCGTTTGGTTTAATGGTACCAATGCTGGCATGTCCGAAAGCAAAATCACTAGGGACATCGACCAAAATGGTACATTCTCAAATGCTTTTAGAGGAAGTCCAAATTGGCCTGCCACTCATGGGACACATGCATATGGCCCCTTGACTGCTGGCCTTCCGAATATACAACCGTTCACGCATCGAATGACTTCTTCTGAATGGCTTCGTCAGTATCGGGAGAATCACAATCTGGAGCGGGATAGTAGTCAGTTAATGCCAGCTCCCTACAATGCTTCAGGAAATCTTATGAACTTCCAAAGAAATGATGCTTCAAGGAATGACTATTTGTATCAAACAGGGAGCCAATTGGGTTATAATCAAACAATGAATATGGAGAGTCCATTGCGCCATCCAGCTTTCCCATTAGCTTATGGAACTAATGAAAACCAGAAAAACATGATCTTCCATGGTTACGAAAGGCCGAACCTTTATGGCTGCGGTGCTACTGACTTGAGAAGTGAGCAGCCGCCGCTTCTGCTGTATCTAAAAGAGAAAGAATGGCAGCTCCAGAAAGATGCTGCTAGCAGAACTCCCACCTATATGGGGAATTGA

Coding sequence (CDS)

ATGGGACGCCTAATTGAGAGATGTTTGAAGGCAACCCAATTGGTTTCTTTCCCTCTTTTACCCGCTGTGCTCGTCTTTGTGGAGTGGTTAGCGAACGTTCTTGATGGAGTATCAAAATATGGTTCTGATGAAAAGAGCAGAAGTTCCATGTCTTACTTTTTTGGTGTTTTTGTTAACCTTCTAGAGAGACTGAATGTTAATACAGTTGACGCAGAGTCTTCACTTGCTATCCCACTGTGGGAAGACTATGAATTAAGGGGCTTCACACCTTTGGCTTCTGCACATGAACCATTAGATTTCTCATCTCACTGGGAACACATGGACAACTACAAATTTGGAGGAAAACATCGTGCTTACCGCATAATTGTTGCTGCTACCAAAATTTCCAACACAGCTAATGATTCTCCAAAGTGTATCATCCACGATAAGACGCGGAAGGTCTTTTACATAGTAGAACAAAATGAACTTGCAGACAAGAAAGCATTGGAAAGTGCAAAGTCGAACATTGTTAGTCCAGATCCGCAAGTGCCAACTCGGGACGTCGGTGAAGATATCCCAGATGAAGTTCAGGATCAGAACCATCTGAATAAGAAATTTGTCACGGTTGAAGATGAAGAAGTCATTCTTTTCAAGCCCCTTATGAGGTATAATTCTGCACCGATCTCTATTGCAGGGAACGGTGAAATTTCACCAAAAAGCGTGGAGGATCAGACCGTTTCTTCTGATGAATGCTTGAGGCGTGCTACATCGCTACTTATAGCACAGACTCAGGGCCAGAGTGATCCCTTTGCTTTTCAAACAGACATTACAAATGTCAACTCAAACAAGTTATCTGAGCAGCATGATACTATGGTAAAAGATACAAGAGAACATCAAATGTCAGAAGGCTCCATATCTGCTGGCCCCCCCTCTCTCAGTGCCTGGGTGCTCAATAGAGGAGGTTTTACTTTGAACCCTGATAGAGAGAAAGGGACAAATGGTTTTGCTAAACCTGGTTTACAGCCGATTGACGAGTTAACTCCGACGTTTATAAATGGTTCTAGACTTGGTGATACCGAGAATTCTGCTTCGAGTCCCAGCCGTGAATCTGGAAAATCATACCAGTTTCCTCCTCCTCCCTATTCAGCCCCAACACCTTCAGCTCCTTATTTACCTGATGATGCCGTTTGGTTTAATGGTACCAATGCTGGCATGTCCGAAAGCAAAATCACTAGGGACATCGACCAAAATGGTACATTCTCAAATGCTTTTAGAGGAAGTCCAAATTGGCCTGCCACTCATGGGACACATGCATATGGCCCCTTGACTGCTGGCCTTCCGAATATACAACCGTTCACGCATCGAATGACTTCTTCTGAATGGCTTCGTCAGTATCGGGAGAATCACAATCTGGAGCGGGATAGTAGTCAGTTAATGCCAGCTCCCTACAATGCTTCAGGAAATCTTATGAACTTCCAAAGAAATGATGCTTCAAGGAATGACTATTTGTATCAAACAGGGAGCCAATTGGGTTATAATCAAACAATGAATATGGAGAGTCCATTGCGCCATCCAGCTTTCCCATTAGCTTATGGAACTAATGAAAACCAGAAAAACATGATCTTCCATGGTTACGAAAGGCCGAACCTTTATGGCTGCGGTGCTACTGACTTGAGAAGTGAGCAGCCGCCGCTTCTGCTGTATCTAAAAGAGAAAGAATGGCAGCTCCAGAAAGATGCTGCTAGCAGAACTCCCACCTATATGGGGAATTGA

Protein sequence

MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNLLERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYRIIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGSPNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN
Homology
BLAST of Moc03g27060 vs. NCBI nr
Match: XP_022139980.1 (protein SMG7L isoform X2 [Momordica charantia])

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL
Sbjct: 404 MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 463

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR
Sbjct: 464 LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 523

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD
Sbjct: 524 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 583

Query: 181 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 240
           VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS
Sbjct: 584 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 643

Query: 241 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 300
           SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA
Sbjct: 644 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 703

Query: 301 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 360
           GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS
Sbjct: 704 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 763

Query: 361 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 420
           RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS
Sbjct: 764 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 823

Query: 421 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 480
           PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG
Sbjct: 824 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 883

Query: 481 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 540
           NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER
Sbjct: 884 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 943

Query: 541 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN
Sbjct: 944 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 986

BLAST of Moc03g27060 vs. NCBI nr
Match: XP_022139975.1 (protein SMG7L isoform X1 [Momordica charantia] >XP_022139976.1 protein SMG7L isoform X1 [Momordica charantia] >XP_022139977.1 protein SMG7L isoform X1 [Momordica charantia] >XP_022139978.1 protein SMG7L isoform X1 [Momordica charantia] >XP_022139979.1 protein SMG7L isoform X1 [Momordica charantia])

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL
Sbjct: 413 MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 472

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR
Sbjct: 473 LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 532

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD
Sbjct: 533 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 592

Query: 181 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 240
           VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS
Sbjct: 593 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 652

Query: 241 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 300
           SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA
Sbjct: 653 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 712

Query: 301 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 360
           GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS
Sbjct: 713 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 772

Query: 361 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 420
           RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS
Sbjct: 773 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 832

Query: 421 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 480
           PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG
Sbjct: 833 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 892

Query: 481 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 540
           NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER
Sbjct: 893 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 952

Query: 541 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN
Sbjct: 953 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 995

BLAST of Moc03g27060 vs. NCBI nr
Match: XP_038876945.1 (protein SMG7L [Benincasa hispida] >XP_038876946.1 protein SMG7L [Benincasa hispida] >XP_038876947.1 protein SMG7L [Benincasa hispida] >XP_038876948.1 protein SMG7L [Benincasa hispida])

HSP 1 Score: 880.6 bits (2274), Expect = 7.7e-252
Identity = 448/594 (75.42%), Postives = 490/594 (82.49%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRL+ERCL+A +L SFPLLPAVLVFVEWL NVL  V + G DEKSRS+M+YFFGV+V L
Sbjct: 405 MGRLVERCLEANKLDSFPLLPAVLVFVEWLPNVLAEVVRNGYDEKSRSAMTYFFGVYVGL 464

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVN V+A+ SLAIPLWEDY LRGFTPLA AHEPLDFSSHWEHMDN+  G KHRAYR
Sbjct: 465 LERLNVNKVEAQCSLAIPLWEDYGLRGFTPLAFAHEPLDFSSHWEHMDNFVLGAKHRAYR 524

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           I VAATKISN ANDSPK IIHD TR+VFY VEQNEL DKKALESAK NIVSPD + PT+D
Sbjct: 525 IFVAATKISNIANDSPKWIIHDMTREVFYTVEQNELQDKKALESAKCNIVSPDLEEPTQD 584

Query: 181 V-------GEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKS 240
           V        ED PDE   QN LNKK V VEDEEVILF PLMRYNSAPISI G+ ++SPKS
Sbjct: 585 VCKDKEECEEDAPDEAWYQNDLNKKSVLVEDEEVILFNPLMRYNSAPISIVGSDDVSPKS 644

Query: 241 VEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTRE-HQ 300
           +E Q+ SSDECLRRATSLLI QTQGQSDPFAF +D TN + NK  EQHD   KDT   HQ
Sbjct: 645 IEAQSTSSDECLRRATSLLIEQTQGQSDPFAFHSDATNFSRNKPFEQHDIFGKDTTAVHQ 704

Query: 301 MSEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDT 360
           +SE S+S GPPSLSAWVLNR GFT +PDREKGTNGF KPGLQPIDELTP F+NG RL DT
Sbjct: 705 ISEASVSTGPPSLSAWVLNR-GFTFDPDREKGTNGFVKPGLQPIDELTPAFMNGLRLDDT 764

Query: 361 ENSASSPSRESGKSYQF-PPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNG 420
           ENSASS S ESGKSY+F PPPPYSAP PSAPYLPDDAVWFNGTNA +S+ KI R+ DQN 
Sbjct: 765 ENSASSLSCESGKSYRFPPPPPYSAPAPSAPYLPDDAVWFNGTNANISDDKIYRERDQNA 824

Query: 421 TFSNAFRGS--PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSS 480
           TFSNAFRGS   NW A H TH Y P+  G  N+ P T+RMTSSEWLRQYRENHNL+ DS+
Sbjct: 825 TFSNAFRGSAYSNWTALHETHQYSPMITGFTNMYPSTYRMTSSEWLRQYRENHNLDGDSN 884

Query: 481 QLMPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNEN 540
           Q++PAPYNA+GNLMNFQRND SR D+LYQTGSQL  N TMNMESPL H AF   Y TNEN
Sbjct: 885 QVLPAPYNATGNLMNFQRNDTSRYDHLYQTGSQLASNPTMNMESPLHHLAFSSTYSTNEN 944

Query: 541 QKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           QKNM+FHG ERPNLYGCGATDLRSEQPPLLL+LK+KEWQLQKDAA+R+  YMGN
Sbjct: 945 QKNMLFHGCERPNLYGCGATDLRSEQPPLLLHLKDKEWQLQKDAANRSAAYMGN 997

BLAST of Moc03g27060 vs. NCBI nr
Match: XP_004146654.1 (protein SMG7L [Cucumis sativus] >XP_011653245.1 protein SMG7L [Cucumis sativus] >XP_031741699.1 protein SMG7L [Cucumis sativus] >XP_031741704.1 protein SMG7L [Cucumis sativus] >KGN64688.1 hypothetical protein Csa_014230 [Cucumis sativus])

HSP 1 Score: 841.6 bits (2173), Expect = 3.9e-240
Identity = 427/594 (71.89%), Postives = 485/594 (81.65%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRL+ERCL+A++L SFPLLPAVL+FVEWL NVLD V +YG DEKSR+SM+YFFGV+V L
Sbjct: 403 MGRLVERCLEASKLDSFPLLPAVLIFVEWLPNVLDEVVRYGDDEKSRNSMTYFFGVYVGL 462

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVN V+A+ SLAIPLWEDYELRGFTPLA +H+PLDFSSHWEHMD ++ G KHRAYR
Sbjct: 463 LERLNVNKVEAQCSLAIPLWEDYELRGFTPLAFSHKPLDFSSHWEHMDTFELGAKHRAYR 522

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISN ANDSPK IIHDKT +VFY ++QNEL DKK LESAK  IVSPD + PT+D
Sbjct: 523 IIVAATKISNIANDSPKWIIHDKTCEVFYTLDQNELPDKKELESAKCYIVSPDLEKPTQD 582

Query: 181 V-------GEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKS 240
           V        ED PDE   Q+ LNKK V VEDEEVILF PLMRYNSAPISIAG+  +SPKS
Sbjct: 583 VFIDKVGCEEDTPDEAWHQSDLNKKSVPVEDEEVILFNPLMRYNSAPISIAGSDNVSPKS 642

Query: 241 VEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQM 300
           VE + +SS+ECLRRATSLLI QTQGQSDPF+F ++ TN + NK  EQH+   KDT  HQ+
Sbjct: 643 VEARAISSNECLRRATSLLIEQTQGQSDPFSFHSNATNFSRNKPFEQHNIFGKDTTGHQI 702

Query: 301 SEGSIS--AGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGD 360
            E SIS   GPPSLSAWVLN  GFT +PDREKGTNGF KPGLQPIDELTPTFING RLGD
Sbjct: 703 PETSISTATGPPSLSAWVLN-NGFTFDPDREKGTNGFVKPGLQPIDELTPTFINGLRLGD 762

Query: 361 TENSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNG 420
           TENSA SPS ES KSY FPPPPYSAP PSAPYLPDDAVWF+ TNA +S+ KI R+ DQN 
Sbjct: 763 TENSALSPSCESRKSYHFPPPPYSAPAPSAPYLPDDAVWFSSTNAIISDGKIYRERDQND 822

Query: 421 TFSNAFRGS--PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSS 480
           T SN+F GS   NW A H TH Y PL +G  N+ P  HRMTSSEWLRQYREN+NL+ +S+
Sbjct: 823 TLSNSFLGSTYSNWSAPHATHEYRPLISGFTNMYPSAHRMTSSEWLRQYRENNNLDGNSN 882

Query: 481 QLMPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNEN 540
           Q++P PYNASGNL +FQRND SR D+LYQT +Q+  N TMN+ESPLRH  FP   G NEN
Sbjct: 883 QVLPTPYNASGNLTDFQRNDTSRYDHLYQTRNQVIPNPTMNIESPLRHLGFPC--GANEN 942

Query: 541 QKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           QK+M FHGYERPNLYGCGATDLRSEQPPL+L+LK+KEW+LQKDAA+R+  YMGN
Sbjct: 943 QKDMFFHGYERPNLYGCGATDLRSEQPPLMLHLKDKEWRLQKDAANRSAAYMGN 993

BLAST of Moc03g27060 vs. NCBI nr
Match: KAA0056998.1 (protein SMG7L [Cucumis melo var. makuwa] >TYK26425.1 protein SMG7L [Cucumis melo var. makuwa])

HSP 1 Score: 839.3 bits (2167), Expect = 2.0e-239
Identity = 425/592 (71.79%), Postives = 477/592 (80.57%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRL+ERCL+A++L SFPL+PAVL+F+EWL NVL+ V +YG DEKSR+SM+YFFGV+V L
Sbjct: 403 MGRLVERCLEASKLDSFPLVPAVLIFMEWLPNVLNEVVRYGDDEKSRNSMTYFFGVYVGL 462

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNV+ V+A+ SLAIPLWEDYELRGFTPLA AH+ LDFSSHWEHMD ++ G KHRAYR
Sbjct: 463 LERLNVDKVEAQCSLAIPLWEDYELRGFTPLAFAHKQLDFSSHWEHMDAFELGAKHRAYR 522

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISN ANDSPK IIHDKT +V Y +EQNEL DKK LESAK  IVSPD + PT+D
Sbjct: 523 IIVAATKISNIANDSPKWIIHDKTCEVVYTLEQNELPDKKELESAKCYIVSPDLEKPTQD 582

Query: 181 V-------GEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKS 240
           V        ED PDE   Q+ LNKK V VEDEEVILF PLMRYNSAPISIA +  +SPKS
Sbjct: 583 VFIDEEGCEEDTPDEAWHQSDLNKKSVPVEDEEVILFNPLMRYNSAPISIAESDNVSPKS 642

Query: 241 VEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQM 300
           VE + +SSDECLRRATSLLI QTQGQSDPF+F ++ TN + NK  EQHD   KD   HQ+
Sbjct: 643 VEARAISSDECLRRATSLLIEQTQGQSDPFSFHSNATNFSRNKPFEQHDIFGKDATGHQI 702

Query: 301 SEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTE 360
            E SIS GPPSLSAWVLN+ GFT +PDREKGTNGF KPGLQPIDELTPTFING RLGDTE
Sbjct: 703 PEASISTGPPSLSAWVLNK-GFTFDPDREKGTNGFVKPGLQPIDELTPTFINGLRLGDTE 762

Query: 361 NSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTF 420
           NS SSPS ES KSY FPPPPYSAP PSAPYLPDDAVWFN TNA +S+ KI ++ DQN T 
Sbjct: 763 NSVSSPSCESRKSYHFPPPPYSAPAPSAPYLPDDAVWFNSTNAIISDGKIYKERDQNDTL 822

Query: 421 SNAFRGS--PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQL 480
           SN F GS   NW   H TH Y PL +G  N+ P  HRMTSSEWLRQYREN NL+ +S+QL
Sbjct: 823 SNVFLGSTYSNWTPPHATHEYSPLISGFTNMYPSAHRMTSSEWLRQYRENQNLDGNSNQL 882

Query: 481 MPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQK 540
           +P PYNASGNL NFQRND SR D+ YQT SQ+  N TMN+ESPLRH  FP   G NENQK
Sbjct: 883 LPTPYNASGNLANFQRNDTSRYDHSYQTRSQVISNPTMNIESPLRHLGFPC--GANENQK 942

Query: 541 NMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           +  FHGYERPNLYGCGATDLRSEQPPLLLYLK+KEW+LQKDAA+R+  YMGN
Sbjct: 943 DTFFHGYERPNLYGCGATDLRSEQPPLLLYLKDKEWRLQKDAANRSAAYMGN 991

BLAST of Moc03g27060 vs. ExPASy Swiss-Prot
Match: Q9FZ99 (Protein SMG7L OS=Arabidopsis thaliana OX=3702 GN=SMG7L PE=2 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 1.1e-54
Identity = 196/603 (32.50%), Postives = 276/603 (45.77%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGS----DEKSRSSMSYFFGV 60
           MGR++ERCLK T L S PLLPA+LVF+++L  +LD V +       DEKS+S++SYFFG 
Sbjct: 373 MGRVVERCLKTTPLDSCPLLPALLVFLDYLPFLLDKVEEEEEECRFDEKSKSAISYFFGK 432

Query: 61  FVNLLERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKH 120
            V++L +L V   +  +   + LWED+EL+   PLA  H  LDFSS+ +  +++  G + 
Sbjct: 433 LVDILNQLKVKDKNCPAKTLLALWEDHELKSLAPLAPIHALLDFSSNMDLRESFDRGKEL 492

Query: 121 RAYRIIVAATKI-SNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQ 180
           R  RII +A  I +     S K +  D  R  FY     EL      E    N    + +
Sbjct: 493 RLQRIISSAIDITTRQKKGSQKWLFFDNQRTHFY-TTSGELQSNG--ELFHGNGEGRNRK 552

Query: 181 VPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVE 240
             T    E IP E       N++ V VE+EEVIL KPL+R  SAPI  +G     P S +
Sbjct: 553 CVTIGPVEIIPLE-------NERSVPVEEEEVILLKPLVRCQSAPIYSSGIA-AKPLSSD 612

Query: 241 -----DQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTRE 300
                +QT +S++ LRR  SL+       S+ F+F   + +               D + 
Sbjct: 613 CTTSGNQTTTSNDSLRRTLSLI------GSESFSFTQGLKDT--------------DPQH 672

Query: 301 HQMSEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKP-GLQPIDELTPTFINGSRL 360
             + EG++S  PPSLSAWV+++       ++EKG  G +KP GL PIDE  P        
Sbjct: 673 LHLEEGTVSGRPPSLSAWVVDK-------NKEKGRLGLSKPNGLGPIDETGPV------- 732

Query: 361 GDTENSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFN---GTNAGMSESKITRD 420
                 ++  S     S + P   YS PTPSAP LP+DA WF+    TN   S    TR 
Sbjct: 733 ------SAFDSLSINSSTEHPASSYSPPTPSAPLLPEDASWFHNDASTNKAESFYDQTRY 792

Query: 421 IDQNGTFSNAFRGSPNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLER 480
           ++  G                            P   P    ++SSEWLR+YRE+ NL  
Sbjct: 793 MELPGIMK-------------------------PYTNPPFVGISSSEWLRRYRESRNLGP 852

Query: 481 DSSQLMPAPYNASG--NLMNFQRNDASRNDYL--YQTGSQLGYNQTMNMESPLRHPAFPL 540
             S      Y A G  NL NF  + +S+   L  Y T +    N T +          P 
Sbjct: 853 AYS------YQAQGTNNLRNFMAHGSSKFSLLARYGTPNDSSQNSTFH----------PQ 880

Query: 541 AYGTNENQKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASR--TPTY 584
            Y  +   +       ++      G +D   +  P L +L+EKEW  +     R   P Y
Sbjct: 913 LYMEDHESRGEKLGNVQQSTTNPYGFSD---DPGPFLRFLREKEWLNENGQRLRGPPPAY 880

BLAST of Moc03g27060 vs. ExPASy Swiss-Prot
Match: A9QM73 (Protein SMG7 OS=Arabidopsis thaliana OX=3702 GN=SMG7 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 2.5e-11
Identity = 67/240 (27.92%), Postives = 97/240 (40.42%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKS---RSSMSYFFGVF 60
           +G +IE+C++     S   LP VLVFVEWLA   D       D++    R+S    F VF
Sbjct: 420 LGLVIEKCVQLGDPSSSYFLPGVLVFVEWLACCPDIALGSDPDDRQTAVRNSFWNQFVVF 479

Query: 61  VNLLERLNVNTVD----------------AESSLAIPLWEDYELRGFTPLASAHEPLDFS 120
            N +  L    +D                 E+   + LWEDYELRGF PL  A   L+FS
Sbjct: 480 FNQVLSLGPTFIDDVEDETCFSNMSLYDERETENRLALWEDYELRGFLPLLPAQTILNFS 539

Query: 121 SHWEHMDNYKFGGKHRAYRIIVAATKISNTANDSPKCIIHDKTRKVFYI-------VEQN 180
                        K R  RI  A   +++        +  D  +K F +          +
Sbjct: 540 RKHSFGTEGPKEKKARIKRIFAAGKALTSVIKVDQNHVYFDSKKKKFLVGVKPADDFLDS 599

Query: 181 ELADKKALESAKSNIVSPDPQVPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLM 215
             +  KA  + + N V  D   P   + + I    +D           +D+EVI+FKPL+
Sbjct: 600 HSSPPKACNALQDNQVMIDHNSPIMQLDQQIYMGEED-----------DDDEVIVFKPLV 648

BLAST of Moc03g27060 vs. ExPASy TrEMBL
Match: A0A6J1CEG5 (protein SMG7L isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010754 PE=4 SV=1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL
Sbjct: 413 MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 472

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR
Sbjct: 473 LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 532

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD
Sbjct: 533 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 592

Query: 181 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 240
           VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS
Sbjct: 593 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 652

Query: 241 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 300
           SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA
Sbjct: 653 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 712

Query: 301 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 360
           GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS
Sbjct: 713 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 772

Query: 361 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 420
           RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS
Sbjct: 773 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 832

Query: 421 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 480
           PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG
Sbjct: 833 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 892

Query: 481 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 540
           NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER
Sbjct: 893 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 952

Query: 541 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN
Sbjct: 953 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 995

BLAST of Moc03g27060 vs. ExPASy TrEMBL
Match: A0A6J1CDS5 (protein SMG7L isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010754 PE=4 SV=1)

HSP 1 Score: 1186.4 bits (3068), Expect = 0.0e+00
Identity = 583/583 (100.00%), Postives = 583/583 (100.00%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL
Sbjct: 404 MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 463

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR
Sbjct: 464 LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 523

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD
Sbjct: 524 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 583

Query: 181 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 240
           VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS
Sbjct: 584 VGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVEDQTVS 643

Query: 241 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 300
           SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA
Sbjct: 644 SDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQMSEGSISA 703

Query: 301 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 360
           GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS
Sbjct: 704 GPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTENSASSPS 763

Query: 361 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 420
           RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS
Sbjct: 764 RESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTFSNAFRGS 823

Query: 421 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 480
           PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG
Sbjct: 824 PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQLMPAPYNASG 883

Query: 481 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 540
           NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER
Sbjct: 884 NLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQKNMIFHGYER 943

Query: 541 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN
Sbjct: 944 PNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 986

BLAST of Moc03g27060 vs. ExPASy TrEMBL
Match: A0A0A0LSD4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G074930 PE=4 SV=1)

HSP 1 Score: 841.6 bits (2173), Expect = 1.9e-240
Identity = 427/594 (71.89%), Postives = 485/594 (81.65%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRL+ERCL+A++L SFPLLPAVL+FVEWL NVLD V +YG DEKSR+SM+YFFGV+V L
Sbjct: 403 MGRLVERCLEASKLDSFPLLPAVLIFVEWLPNVLDEVVRYGDDEKSRNSMTYFFGVYVGL 462

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNVN V+A+ SLAIPLWEDYELRGFTPLA +H+PLDFSSHWEHMD ++ G KHRAYR
Sbjct: 463 LERLNVNKVEAQCSLAIPLWEDYELRGFTPLAFSHKPLDFSSHWEHMDTFELGAKHRAYR 522

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISN ANDSPK IIHDKT +VFY ++QNEL DKK LESAK  IVSPD + PT+D
Sbjct: 523 IIVAATKISNIANDSPKWIIHDKTCEVFYTLDQNELPDKKELESAKCYIVSPDLEKPTQD 582

Query: 181 V-------GEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKS 240
           V        ED PDE   Q+ LNKK V VEDEEVILF PLMRYNSAPISIAG+  +SPKS
Sbjct: 583 VFIDKVGCEEDTPDEAWHQSDLNKKSVPVEDEEVILFNPLMRYNSAPISIAGSDNVSPKS 642

Query: 241 VEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQM 300
           VE + +SS+ECLRRATSLLI QTQGQSDPF+F ++ TN + NK  EQH+   KDT  HQ+
Sbjct: 643 VEARAISSNECLRRATSLLIEQTQGQSDPFSFHSNATNFSRNKPFEQHNIFGKDTTGHQI 702

Query: 301 SEGSIS--AGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGD 360
            E SIS   GPPSLSAWVLN  GFT +PDREKGTNGF KPGLQPIDELTPTFING RLGD
Sbjct: 703 PETSISTATGPPSLSAWVLN-NGFTFDPDREKGTNGFVKPGLQPIDELTPTFINGLRLGD 762

Query: 361 TENSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNG 420
           TENSA SPS ES KSY FPPPPYSAP PSAPYLPDDAVWF+ TNA +S+ KI R+ DQN 
Sbjct: 763 TENSALSPSCESRKSYHFPPPPYSAPAPSAPYLPDDAVWFSSTNAIISDGKIYRERDQND 822

Query: 421 TFSNAFRGS--PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSS 480
           T SN+F GS   NW A H TH Y PL +G  N+ P  HRMTSSEWLRQYREN+NL+ +S+
Sbjct: 823 TLSNSFLGSTYSNWSAPHATHEYRPLISGFTNMYPSAHRMTSSEWLRQYRENNNLDGNSN 882

Query: 481 QLMPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNEN 540
           Q++P PYNASGNL +FQRND SR D+LYQT +Q+  N TMN+ESPLRH  FP   G NEN
Sbjct: 883 QVLPTPYNASGNLTDFQRNDTSRYDHLYQTRNQVIPNPTMNIESPLRHLGFPC--GANEN 942

Query: 541 QKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           QK+M FHGYERPNLYGCGATDLRSEQPPL+L+LK+KEW+LQKDAA+R+  YMGN
Sbjct: 943 QKDMFFHGYERPNLYGCGATDLRSEQPPLMLHLKDKEWRLQKDAANRSAAYMGN 993

BLAST of Moc03g27060 vs. ExPASy TrEMBL
Match: A0A5A7UPF8 (Protein SMG7L OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00890 PE=4 SV=1)

HSP 1 Score: 839.3 bits (2167), Expect = 9.5e-240
Identity = 425/592 (71.79%), Postives = 477/592 (80.57%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRL+ERCL+A++L SFPL+PAVL+F+EWL NVL+ V +YG DEKSR+SM+YFFGV+V L
Sbjct: 403 MGRLVERCLEASKLDSFPLVPAVLIFMEWLPNVLNEVVRYGDDEKSRNSMTYFFGVYVGL 462

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNV+ V+A+ SLAIPLWEDYELRGFTPLA AH+ LDFSSHWEHMD ++ G KHRAYR
Sbjct: 463 LERLNVDKVEAQCSLAIPLWEDYELRGFTPLAFAHKQLDFSSHWEHMDAFELGAKHRAYR 522

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISN ANDSPK IIHDKT +V Y +EQNEL DKK LESAK  IVSPD + PT+D
Sbjct: 523 IIVAATKISNIANDSPKWIIHDKTCEVVYTLEQNELPDKKELESAKCYIVSPDLEKPTQD 582

Query: 181 V-------GEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKS 240
           V        ED PDE   Q+ LNKK V VEDEEVILF PLMRYNSAPISIA +  +SPKS
Sbjct: 583 VFIDEEGCEEDTPDEAWHQSDLNKKSVPVEDEEVILFNPLMRYNSAPISIAESDNVSPKS 642

Query: 241 VEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQM 300
           VE + +SSDECLRRATSLLI QTQGQSDPF+F ++ TN + NK  EQHD   KD   HQ+
Sbjct: 643 VEARAISSDECLRRATSLLIEQTQGQSDPFSFHSNATNFSRNKPFEQHDIFGKDATGHQI 702

Query: 301 SEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTE 360
            E SIS GPPSLSAWVLN+ GFT +PDREKGTNGF KPGLQPIDELTPTFING RLGDTE
Sbjct: 703 PEASISTGPPSLSAWVLNK-GFTFDPDREKGTNGFVKPGLQPIDELTPTFINGLRLGDTE 762

Query: 361 NSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTF 420
           NS SSPS ES KSY FPPPPYSAP PSAPYLPDDAVWFN TNA +S+ KI ++ DQN T 
Sbjct: 763 NSVSSPSCESRKSYHFPPPPYSAPAPSAPYLPDDAVWFNSTNAIISDGKIYKERDQNDTL 822

Query: 421 SNAFRGS--PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQL 480
           SN F GS   NW   H TH Y PL +G  N+ P  HRMTSSEWLRQYREN NL+ +S+QL
Sbjct: 823 SNVFLGSTYSNWTPPHATHEYSPLISGFTNMYPSAHRMTSSEWLRQYRENQNLDGNSNQL 882

Query: 481 MPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQK 540
           +P PYNASGNL NFQRND SR D+ YQT SQ+  N TMN+ESPLRH  FP   G NENQK
Sbjct: 883 LPTPYNASGNLANFQRNDTSRYDHSYQTRSQVISNPTMNIESPLRHLGFPC--GANENQK 942

Query: 541 NMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           +  FHGYERPNLYGCGATDLRSEQPPLLLYLK+KEW+LQKDAA+R+  YMGN
Sbjct: 943 DTFFHGYERPNLYGCGATDLRSEQPPLLLYLKDKEWRLQKDAANRSAAYMGN 991

BLAST of Moc03g27060 vs. ExPASy TrEMBL
Match: A0A1S3B720 (LOW QUALITY PROTEIN: protein SMG7L OS=Cucumis melo OX=3656 GN=LOC103486485 PE=4 SV=1)

HSP 1 Score: 836.6 bits (2160), Expect = 6.1e-239
Identity = 424/592 (71.62%), Postives = 476/592 (80.41%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKSRSSMSYFFGVFVNL 60
           MGRL+ERCL+A++L SFPL+PAVL+F+EWL NVL+ V +YG DEKSR+SM+Y FGV+V L
Sbjct: 403 MGRLVERCLEASKLDSFPLVPAVLIFMEWLPNVLNEVVRYGDDEKSRNSMTYXFGVYVGL 462

Query: 61  LERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKHRAYR 120
           LERLNV+ V+A+ SLAIPLWEDYELRGFTPLA AH+ LDFSSHWEHMD ++ G KHRAYR
Sbjct: 463 LERLNVDKVEAQCSLAIPLWEDYELRGFTPLAFAHKQLDFSSHWEHMDAFELGAKHRAYR 522

Query: 121 IIVAATKISNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQVPTRD 180
           IIVAATKISN ANDSPK IIHDKT +V Y +EQNEL DKK LESAK  IVSPD + PT+D
Sbjct: 523 IIVAATKISNIANDSPKWIIHDKTCEVVYTLEQNELPDKKELESAKCYIVSPDLEKPTQD 582

Query: 181 V-------GEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKS 240
           V        ED PDE   Q+ LNKK V VEDEEVILF PLMRYNSAPISIA +  +SPKS
Sbjct: 583 VFIDEEGCEEDTPDEAWHQSDLNKKSVPVEDEEVILFNPLMRYNSAPISIAESDNVSPKS 642

Query: 241 VEDQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTREHQM 300
           VE + +SSDECLRRATSLLI QTQGQSDPF+F ++ TN + NK  EQHD   KD   HQ+
Sbjct: 643 VEARAISSDECLRRATSLLIEQTQGQSDPFSFHSNATNFSRNKPFEQHDIFGKDATGHQI 702

Query: 301 SEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKPGLQPIDELTPTFINGSRLGDTE 360
            E SIS GPPSLSAWVLN+ GFT +PDREKGTNGF KPGLQPIDELTPTFING RLGDTE
Sbjct: 703 PEASISTGPPSLSAWVLNK-GFTFDPDREKGTNGFVKPGLQPIDELTPTFINGLRLGDTE 762

Query: 361 NSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFNGTNAGMSESKITRDIDQNGTF 420
           NS SSPS ES KSY FPPPPYSAP PSAPYLPDDAVWFN TNA +S+ KI ++ DQN T 
Sbjct: 763 NSVSSPSCESRKSYHFPPPPYSAPAPSAPYLPDDAVWFNSTNAIISDGKIYKERDQNDTL 822

Query: 421 SNAFRGS--PNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLERDSSQL 480
           SN F GS   NW   H TH Y PL +G  N+ P  HRMTSSEWLRQYREN NL+ +S+QL
Sbjct: 823 SNVFLGSTYSNWTPPHATHEYSPLISGFTNMYPSAHRMTSSEWLRQYRENQNLDGNSNQL 882

Query: 481 MPAPYNASGNLMNFQRNDASRNDYLYQTGSQLGYNQTMNMESPLRHPAFPLAYGTNENQK 540
           +P PYNASGNL NFQRND SR D+ YQT SQ+  N TMN+ESPLRH  FP   G NENQK
Sbjct: 883 LPTPYNASGNLANFQRNDTSRYDHSYQTRSQVISNPTMNIESPLRHLGFPC--GANENQK 942

Query: 541 NMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASRTPTYMGN 584
           +  FHGYERPNLYGCGATDLRSEQPPLLLYLK+KEW+LQKDAA+R+  YMGN
Sbjct: 943 DTFFHGYERPNLYGCGATDLRSEQPPLLLYLKDKEWRLQKDAANRSAAYMGN 991

BLAST of Moc03g27060 vs. TAIR 10
Match: AT1G28260.1 (Telomerase activating protein Est1 )

HSP 1 Score: 216.1 bits (549), Expect = 7.6e-56
Identity = 196/603 (32.50%), Postives = 276/603 (45.77%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGS----DEKSRSSMSYFFGV 60
           MGR++ERCLK T L S PLLPA+LVF+++L  +LD V +       DEKS+S++SYFFG 
Sbjct: 373 MGRVVERCLKTTPLDSCPLLPALLVFLDYLPFLLDKVEEEEEECRFDEKSKSAISYFFGK 432

Query: 61  FVNLLERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKH 120
            V++L +L V   +  +   + LWED+EL+   PLA  H  LDFSS+ +  +++  G + 
Sbjct: 433 LVDILNQLKVKDKNCPAKTLLALWEDHELKSLAPLAPIHALLDFSSNMDLRESFDRGKEL 492

Query: 121 RAYRIIVAATKI-SNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQ 180
           R  RII +A  I +     S K +  D  R  FY     EL      E    N    + +
Sbjct: 493 RLQRIISSAIDITTRQKKGSQKWLFFDNQRTHFY-TTSGELQSNG--ELFHGNGEGRNRK 552

Query: 181 VPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVE 240
             T    E IP E       N++ V VE+EEVIL KPL+R  SAPI  +G     P S +
Sbjct: 553 CVTIGPVEIIPLE-------NERSVPVEEEEVILLKPLVRCQSAPIYSSGIA-AKPLSSD 612

Query: 241 -----DQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTRE 300
                +QT +S++ LRR  SL+       S+ F+F   + +               D + 
Sbjct: 613 CTTSGNQTTTSNDSLRRTLSLI------GSESFSFTQGLKDT--------------DPQH 672

Query: 301 HQMSEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKP-GLQPIDELTPTFINGSRL 360
             + EG++S  PPSLSAWV+++       ++EKG  G +KP GL PIDE  P        
Sbjct: 673 LHLEEGTVSGRPPSLSAWVVDK-------NKEKGRLGLSKPNGLGPIDETGPV------- 732

Query: 361 GDTENSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFN---GTNAGMSESKITRD 420
                 ++  S     S + P   YS PTPSAP LP+DA WF+    TN   S    TR 
Sbjct: 733 ------SAFDSLSINSSTEHPASSYSPPTPSAPLLPEDASWFHNDASTNKAESFYDQTRY 792

Query: 421 IDQNGTFSNAFRGSPNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLER 480
           ++  G                            P   P    ++SSEWLR+YRE+ NL  
Sbjct: 793 MELPGIMK-------------------------PYTNPPFVGISSSEWLRRYRESRNLGP 852

Query: 481 DSSQLMPAPYNASG--NLMNFQRNDASRNDYL--YQTGSQLGYNQTMNMESPLRHPAFPL 540
             S      Y A G  NL NF  + +S+   L  Y T +    N T +          P 
Sbjct: 853 AYS------YQAQGTNNLRNFMAHGSSKFSLLARYGTPNDSSQNSTFH----------PQ 880

Query: 541 AYGTNENQKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASR--TPTY 584
            Y  +   +       ++      G +D   +  P L +L+EKEW  +     R   P Y
Sbjct: 913 LYMEDHESRGEKLGNVQQSTTNPYGFSD---DPGPFLRFLREKEWLNENGQRLRGPPPAY 880

BLAST of Moc03g27060 vs. TAIR 10
Match: AT1G28260.2 (Telomerase activating protein Est1 )

HSP 1 Score: 216.1 bits (549), Expect = 7.6e-56
Identity = 196/603 (32.50%), Postives = 276/603 (45.77%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGS----DEKSRSSMSYFFGV 60
           MGR++ERCLK T L S PLLPA+LVF+++L  +LD V +       DEKS+S++SYFFG 
Sbjct: 373 MGRVVERCLKTTPLDSCPLLPALLVFLDYLPFLLDKVEEEEEECRFDEKSKSAISYFFGK 432

Query: 61  FVNLLERLNVNTVDAESSLAIPLWEDYELRGFTPLASAHEPLDFSSHWEHMDNYKFGGKH 120
            V++L +L V   +  +   + LWED+EL+   PLA  H  LDFSS+ +  +++  G + 
Sbjct: 433 LVDILNQLKVKDKNCPAKTLLALWEDHELKSLAPLAPIHALLDFSSNMDLRESFDRGKEL 492

Query: 121 RAYRIIVAATKI-SNTANDSPKCIIHDKTRKVFYIVEQNELADKKALESAKSNIVSPDPQ 180
           R  RII +A  I +     S K +  D  R  FY     EL      E    N    + +
Sbjct: 493 RLQRIISSAIDITTRQKKGSQKWLFFDNQRTHFY-TTSGELQSNG--ELFHGNGEGRNRK 552

Query: 181 VPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLMRYNSAPISIAGNGEISPKSVE 240
             T    E IP E       N++ V VE+EEVIL KPL+R  SAPI  +G     P S +
Sbjct: 553 CVTIGPVEIIPLE-------NERSVPVEEEEVILLKPLVRCQSAPIYSSGIA-AKPLSSD 612

Query: 241 -----DQTVSSDECLRRATSLLIAQTQGQSDPFAFQTDITNVNSNKLSEQHDTMVKDTRE 300
                +QT +S++ LRR  SL+       S+ F+F   + +               D + 
Sbjct: 613 CTTSGNQTTTSNDSLRRTLSLI------GSESFSFTQGLKDT--------------DPQH 672

Query: 301 HQMSEGSISAGPPSLSAWVLNRGGFTLNPDREKGTNGFAKP-GLQPIDELTPTFINGSRL 360
             + EG++S  PPSLSAWV+++       ++EKG  G +KP GL PIDE  P        
Sbjct: 673 LHLEEGTVSGRPPSLSAWVVDK-------NKEKGRLGLSKPNGLGPIDETGPV------- 732

Query: 361 GDTENSASSPSRESGKSYQFPPPPYSAPTPSAPYLPDDAVWFN---GTNAGMSESKITRD 420
                 ++  S     S + P   YS PTPSAP LP+DA WF+    TN   S    TR 
Sbjct: 733 ------SAFDSLSINSSTEHPASSYSPPTPSAPLLPEDASWFHNDASTNKAESFYDQTRY 792

Query: 421 IDQNGTFSNAFRGSPNWPATHGTHAYGPLTAGLPNIQPFTHRMTSSEWLRQYRENHNLER 480
           ++  G                            P   P    ++SSEWLR+YRE+ NL  
Sbjct: 793 MELPGIMK-------------------------PYTNPPFVGISSSEWLRRYRESRNLGP 852

Query: 481 DSSQLMPAPYNASG--NLMNFQRNDASRNDYL--YQTGSQLGYNQTMNMESPLRHPAFPL 540
             S      Y A G  NL NF  + +S+   L  Y T +    N T +          P 
Sbjct: 853 AYS------YQAQGTNNLRNFMAHGSSKFSLLARYGTPNDSSQNSTFH----------PQ 880

Query: 541 AYGTNENQKNMIFHGYERPNLYGCGATDLRSEQPPLLLYLKEKEWQLQKDAASR--TPTY 584
            Y  +   +       ++      G +D   +  P L +L+EKEW  +     R   P Y
Sbjct: 913 LYMEDHESRGEKLGNVQQSTTNPYGFSD---DPGPFLRFLREKEWLNENGQRLRGPPPAY 880

BLAST of Moc03g27060 vs. TAIR 10
Match: AT5G19400.1 (Telomerase activating protein Est1 )

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-12
Identity = 67/240 (27.92%), Postives = 97/240 (40.42%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKS---RSSMSYFFGVF 60
           +G +IE+C++     S   LP VLVFVEWLA   D       D++    R+S    F VF
Sbjct: 420 LGLVIEKCVQLGDPSSSYFLPGVLVFVEWLACCPDIALGSDPDDRQTAVRNSFWNQFVVF 479

Query: 61  VNLLERLNVNTVD----------------AESSLAIPLWEDYELRGFTPLASAHEPLDFS 120
            N +  L    +D                 E+   + LWEDYELRGF PL  A   L+FS
Sbjct: 480 FNQVLSLGPTFIDDVEDETCFSNMSLYDERETENRLALWEDYELRGFLPLLPAQTILNFS 539

Query: 121 SHWEHMDNYKFGGKHRAYRIIVAATKISNTANDSPKCIIHDKTRKVFYI-------VEQN 180
                        K R  RI  A   +++        +  D  +K F +          +
Sbjct: 540 RKHSFGTEGPKEKKARIKRIFAAGKALTSVIKVDQNHVYFDSKKKKFLVGVKPADDFLDS 599

Query: 181 ELADKKALESAKSNIVSPDPQVPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLM 215
             +  KA  + + N V  D   P   + + I    +D           +D+EVI+FKPL+
Sbjct: 600 HSSPPKACNALQDNQVMIDHNSPIMQLDQQIYMGEED-----------DDDEVIVFKPLV 648

BLAST of Moc03g27060 vs. TAIR 10
Match: AT5G19400.3 (Telomerase activating protein Est1 )

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-12
Identity = 67/240 (27.92%), Postives = 97/240 (40.42%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKS---RSSMSYFFGVF 60
           +G +IE+C++     S   LP VLVFVEWLA   D       D++    R+S    F VF
Sbjct: 420 LGLVIEKCVQLGDPSSSYFLPGVLVFVEWLACCPDIALGSDPDDRQTAVRNSFWNQFVVF 479

Query: 61  VNLLERLNVNTVD----------------AESSLAIPLWEDYELRGFTPLASAHEPLDFS 120
            N +  L    +D                 E+   + LWEDYELRGF PL  A   L+FS
Sbjct: 480 FNQVLSLGPTFIDDVEDETCFSNMSLYDERETENRLALWEDYELRGFLPLLPAQTILNFS 539

Query: 121 SHWEHMDNYKFGGKHRAYRIIVAATKISNTANDSPKCIIHDKTRKVFYI-------VEQN 180
                        K R  RI  A   +++        +  D  +K F +          +
Sbjct: 540 RKHSFGTEGPKEKKARIKRIFAAGKALTSVIKVDQNHVYFDSKKKKFLVGVKPADDFLDS 599

Query: 181 ELADKKALESAKSNIVSPDPQVPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLM 215
             +  KA  + + N V  D   P   + + I    +D           +D+EVI+FKPL+
Sbjct: 600 HSSPPKACNALQDNQVMIDHNSPIMQLDQQIYMGEED-----------DDDEVIVFKPLV 648

BLAST of Moc03g27060 vs. TAIR 10
Match: AT5G19400.2 (Telomerase activating protein Est1 )

HSP 1 Score: 72.0 bits (175), Expect = 1.8e-12
Identity = 67/240 (27.92%), Postives = 97/240 (40.42%), Query Frame = 0

Query: 1   MGRLIERCLKATQLVSFPLLPAVLVFVEWLANVLDGVSKYGSDEKS---RSSMSYFFGVF 60
           +G +IE+C++     S   LP VLVFVEWLA   D       D++    R+S    F VF
Sbjct: 420 LGLVIEKCVQLGDPSSSYFLPGVLVFVEWLACCPDIALGSDPDDRQTAVRNSFWNQFVVF 479

Query: 61  VNLLERLNVNTVD----------------AESSLAIPLWEDYELRGFTPLASAHEPLDFS 120
            N +  L    +D                 E+   + LWEDYELRGF PL  A   L+FS
Sbjct: 480 FNQVLSLGPTFIDDVEDETCFSNMSLYDERETENRLALWEDYELRGFLPLLPAQTILNFS 539

Query: 121 SHWEHMDNYKFGGKHRAYRIIVAATKISNTANDSPKCIIHDKTRKVFYI-------VEQN 180
                        K R  RI  A   +++        +  D  +K F +          +
Sbjct: 540 RKHSFGTEGPKEKKARIKRIFAAGKALTSVIKVDQNHVYFDSKKKKFLVGVKPADDFLDS 599

Query: 181 ELADKKALESAKSNIVSPDPQVPTRDVGEDIPDEVQDQNHLNKKFVTVEDEEVILFKPLM 215
             +  KA  + + N V  D   P   + + I    +D           +D+EVI+FKPL+
Sbjct: 600 HSSPPKACNALQDNQVMIDHNSPIMQLDQQIYMGEED-----------DDDEVIVFKPLV 648

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022139980.10.0e+00100.00protein SMG7L isoform X2 [Momordica charantia][more]
XP_022139975.10.0e+00100.00protein SMG7L isoform X1 [Momordica charantia] >XP_022139976.1 protein SMG7L iso... [more]
XP_038876945.17.7e-25275.42protein SMG7L [Benincasa hispida] >XP_038876946.1 protein SMG7L [Benincasa hispi... [more]
XP_004146654.13.9e-24071.89protein SMG7L [Cucumis sativus] >XP_011653245.1 protein SMG7L [Cucumis sativus] ... [more]
KAA0056998.12.0e-23971.79protein SMG7L [Cucumis melo var. makuwa] >TYK26425.1 protein SMG7L [Cucumis melo... [more]
Match NameE-valueIdentityDescription
Q9FZ991.1e-5432.50Protein SMG7L OS=Arabidopsis thaliana OX=3702 GN=SMG7L PE=2 SV=1[more]
A9QM732.5e-1127.92Protein SMG7 OS=Arabidopsis thaliana OX=3702 GN=SMG7 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CEG50.0e+00100.00protein SMG7L isoform X1 OS=Momordica charantia OX=3673 GN=LOC111010754 PE=4 SV=... [more]
A0A6J1CDS50.0e+00100.00protein SMG7L isoform X2 OS=Momordica charantia OX=3673 GN=LOC111010754 PE=4 SV=... [more]
A0A0A0LSD41.9e-24071.89Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G074930 PE=4 SV=1[more]
A0A5A7UPF89.5e-24071.79Protein SMG7L OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold861G00890 ... [more]
A0A1S3B7206.1e-23971.62LOW QUALITY PROTEIN: protein SMG7L OS=Cucumis melo OX=3656 GN=LOC103486485 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT1G28260.17.6e-5632.50Telomerase activating protein Est1 [more]
AT1G28260.27.6e-5632.50Telomerase activating protein Est1 [more]
AT5G19400.11.8e-1227.92Telomerase activating protein Est1 [more]
AT5G19400.31.8e-1227.92Telomerase activating protein Est1 [more]
AT5G19400.21.8e-1227.92Telomerase activating protein Est1 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR018834DNA/RNA-binding domain, Est1-typePFAMPF10373EST1_DNA_bindcoord: 1..94
e-value: 3.3E-12
score: 46.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 345..365
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 319..381
NoneNo IPR availablePANTHERPTHR15696:SF27BNAC03G58470D PROTEINcoord: 1..153
NoneNo IPR availablePANTHERPTHR15696:SF27BNAC03G58470D PROTEINcoord: 198..392
IPR045153Est1/Ebs1-likePANTHERPTHR15696SMG-7 SUPPRESSOR WITH MORPHOLOGICAL EFFECT ON GENITALIA PROTEIN 7coord: 1..153
coord: 198..392
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 15..131

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g27060.1Moc03g27060.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000184 nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
cellular_component GO:0005697 telomerase holoenzyme complex
molecular_function GO:0005515 protein binding
molecular_function GO:0070034 telomerase RNA binding
molecular_function GO:0042162 telomeric DNA binding