Moc03g25890 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g25890
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Gibberellin 2-beta-dioxygenase-like
Location	chr3: 18621101 .. 18623930 (-)
RNA-Seq Expression	Moc03g25890
Synteny	Moc03g25890

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGGTCCAGTCTCAGCTGGTGTTCGACCATTTTGCTCCGATCAGGTCGTGCAAACGCACCGCCGTGTTCGAGGGGATTCCGGTGATCGACCTGTCGGACCCGGACGCGAAGACCCACATCGTGAAGGCCTGCGAGGACTTCGGGTTCTTCAAGGTCGTCAACCATGGCGTCTCCGACGAGCTCATCGCCGCCATCGAAGCCCAAGCTCGGAGATTCTTCGAGCTCCCACAGATGGAGAAGGAGAAAGCAGGGCCGCCCGACCCATTTGGATATGGCAGCAAGCGAATTGGCCCCAACGGCGACGTGGGTTGGATCGAATACCTCCTCCTCAACACCAATCCCCAACTCATTTCTCAAAAATCCCTCTTCATTTTCCAAGAAAATCCCGATATTTTCAGGTACCAACACAACAGAGTACTCTGTTTTACTCTGTTTTAGAGTTAGTGCTCTGTTTTCTGGAACAGAGGTGATAAATTGTTGTATTTGTTTTGGTTTGCAGGTGTGCTGTGGAGGAGTATATAGGGGCAGTGAAGGGGATGGCTTGTGAAGTGCTGGAAATGATGGCGGAAGGGCTGGGGATTGAGAAAAGTGCCTTGAGCAGGCTGCTGAGGGACGAGAGAAGTGACTGCTGTTTCAGGATGAATCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGAAACTTGATAGGCTTTGGGGAACACACAGACCCACAAATAATATCTGTCTTGAGATCAAATAACACAACTGGACTCCATATCTGTTTAAGGGATGGGAGTTGGGTCTCAGTCCCACCTGATCAGACCTCCTTTTTCATCAATATTGGTGACTCATTACAGGTACAATCCTCTTTTTTTTTTTTTCTCTGCCTTTCTCATGTGGGGAAATTCAAAATGGAGCTCCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCCTTCTTGTTTGTTCATTGTTCTTGTTGTTTCTCTTTCTGGGATCTCATTGTAATGGGTGGTTTTTGTGTTGTGTTGTTGATGCAGGTGATGACTAATGGGAGGTTTAAGAGTGTGAAGCACAAGGTTTTAGCTGATCCATTAAAATCAAGGTTTTCAATGATCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTGCCATCACTATTGGAGGAAGGAGAGCAAAGTTTGTACAGAGAGTTCACTTGGTGGGATTACAAAATCTCTGCATATAAATCAAGGCTTGCAGACTGTAGGTTGGGCCCTTTTGAGAAAAAAAATTTAACACCTCTGCTGACCTGAGAAACCCAACAAAAATAATTAAAAAAAAAAAAGAAACTTCCTTTTATGTCACAATGCAAAGAGATAGAAACAAGAGAGAGAGGCTGTTTTGGGATATTTTGAAGATATGTGAAAATTCTTCTGTCATTTCTCTTTTATTCTTATATGTGGTTATGGTTTGGGGACATGAAGTGAAAAAAGCAAAAATGGGTGTAAATCATAATCACATGATAAAATAGTGGAGAAATAATAGATGAATTTTTTTTTTTTTTTTTTTTGAAAGAAAGTGAGAATTAGAGAGGAAAGGTAGAGATAGAGATGGTAGGGAGGGAGGGATTTAGGGAAGGTTAGAAAACCAATAGAATAGGTTTGTTTTTGTTTTCTCATATGTTGTTCTTTTTGTTTGTTAAATATTTGCTTTTGGGTTTAAATGTAATAGTGAAGGACACCCACTTTAATTCCATCCTCCAATTCCATTAATGCCCTTGTCATCATCATGTCACCATGTCCAAATGACACCCTCGCCCTATGCTATGTTCCCCAAAATACCCTTCACTTGTAAATATTCTTGTCACATGAATTACCCTTACCCCCAATCCCCCTATGTATCAAAGCTTGTGGATTTCAATGTTATAATGTAATATATGTGCTTCTTGCAATTTCCCACCTCTCACAATGTAAACTTTCTCCTTTCAAATTATGTTTATGTTAACGACGTAAATGACCATAATATCAAAATGACGTCGAAAAAATAAGAGATCTAACAAAAGTTTATAATAGACATCGATAAGGTGATTTTGAAATGAAATCTATGAGTATTTAAAATATCTTTACATTCGATAATTAATCGAAGAGAATGTGTAAATCGAGCGTCAGCTGGAGGAATATCCCAACATGGACATTGAAAGAAAGAAGTGATGTTGACGAGGTTGGAAAGAGAGAGGAAAAGGGGAAGGTGAGGGTGAGGGTGGGGCACAATTGAGAATAAAGGAAGAAATTTTGTGAAAGGAAGGGGAGGGTTTTCCTTGTGGAGGGCATAACACAGCACCAACACCAAAACCAAAGGGAGTCATTTTCAGACAGGCTGCGTTTGACCCCTTCAATTTTGTAATTAGAAAGGCCATTGCAAGAAAGAATATTGAATATGGCCGCCACAATGTAACTGCGATGGAGACTAAAAAGTATCGATTTTATGATGACTATTTGATATAATTTTGAAATACTTTCGTAATTTTTTTTATACAGTATAAGGGTGCGTATGAAAATAGTCAAAGTATCACTTGGATGATGATTTTGGAAAATGCTCAACAACCACTTGGATGATGATTTTGGAAAATGCTTAACGTTGTTGAGTGACGTGTACTGGACCCTAACAAACTGAGGGAGAGCGAGCTAGGAGGAAGAGACCTGCAAAAATAGGTAGAACACTAAGACACTCAAGTTAGTCCTTAATTCAGATTTTCATACTTGGTTAGGCGAAGTGATGATGTGTTTATAAGGGATGAGTGGGTTGTTACGGTAGAACTGTTTCCAAGATCAGTCACCGACTTTTCATCGTAG

mRNA sequence

ATGGTGGTCCAGTCTCAGCTGGTGTTCGACCATTTTGCTCCGATCAGGTCGTGCAAACGCACCGCCGTGTTCGAGGGGATTCCGGTGATCGACCTGTCGGACCCGGACGCGAAGACCCACATCGTGAAGGCCTGCGAGGACTTCGGGTTCTTCAAGGTCGTCAACCATGGCGTCTCCGACGAGCTCATCGCCGCCATCGAAGCCCAAGCTCGGAGATTCTTCGAGCTCCCACAGATGGAGAAGGAGAAAGCAGGGCCGCCCGACCCATTTGGATATGGCAGCAAGCGAATTGGCCCCAACGGCGACGTGGGTTGGATCGAATACCTCCTCCTCAACACCAATCCCCAACTCATTTCTCAAAAATCCCTCTTCATTTTCCAAGAAAATCCCGATATTTTCAGGTGTGCTGTGGAGGAGTATATAGGGGCAGTGAAGGGGATGGCTTGTGAAGTGCTGGAAATGATGGCGGAAGGGCTGGGGATTGAGAAAAGTGCCTTGAGCAGGCTGCTGAGGGACGAGAGAAGTGACTGCTGTTTCAGGATGAATCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGAAACTTGATAGGCTTTGGGGAACACACAGACCCACAAATAATATCTGTCTTGAGATCAAATAACACAACTGGACTCCATATCTGTTTAAGGGATGGGAGTTGGGTCTCAGTCCCACCTGATCAGACCTCCTTTTTCATCAATATTGGTGACTCATTACAGGTGATGACTAATGGGAGGTTTAAGAGTGTGAAGCACAAGGTTTTAGCTGATCCATTAAAATCAAGGTTTTCAATGATCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTGCCATCACTATTGGAGGAAGGAGAGCAAAGTTTGTACAGAGAGTTCACTTGGTGGGATTACAAAATCTCTGCATATAAATCAAGGCTTGCAGACTGTAGGCGAAGTGATGATGTGTTTATAAGGGATGAGTGGGTTGTTACGGTAGAACTGTTTCCAAGATCAGTCACCGACTTTTCATCGTAG

Coding sequence (CDS)

ATGGTGGTCCAGTCTCAGCTGGTGTTCGACCATTTTGCTCCGATCAGGTCGTGCAAACGCACCGCCGTGTTCGAGGGGATTCCGGTGATCGACCTGTCGGACCCGGACGCGAAGACCCACATCGTGAAGGCCTGCGAGGACTTCGGGTTCTTCAAGGTCGTCAACCATGGCGTCTCCGACGAGCTCATCGCCGCCATCGAAGCCCAAGCTCGGAGATTCTTCGAGCTCCCACAGATGGAGAAGGAGAAAGCAGGGCCGCCCGACCCATTTGGATATGGCAGCAAGCGAATTGGCCCCAACGGCGACGTGGGTTGGATCGAATACCTCCTCCTCAACACCAATCCCCAACTCATTTCTCAAAAATCCCTCTTCATTTTCCAAGAAAATCCCGATATTTTCAGGTGTGCTGTGGAGGAGTATATAGGGGCAGTGAAGGGGATGGCTTGTGAAGTGCTGGAAATGATGGCGGAAGGGCTGGGGATTGAGAAAAGTGCCTTGAGCAGGCTGCTGAGGGACGAGAGAAGTGACTGCTGTTTCAGGATGAATCATTATCCGCCATGCCCGGAGCTTGAAGCTTTGAGTGGCAGAAACTTGATAGGCTTTGGGGAACACACAGACCCACAAATAATATCTGTCTTGAGATCAAATAACACAACTGGACTCCATATCTGTTTAAGGGATGGGAGTTGGGTCTCAGTCCCACCTGATCAGACCTCCTTTTTCATCAATATTGGTGACTCATTACAGGTGATGACTAATGGGAGGTTTAAGAGTGTGAAGCACAAGGTTTTAGCTGATCCATTAAAATCAAGGTTTTCAATGATCTACTTTGGTGGGCCACCTTTGAATGAAAAGATTGCACCTTTGCCATCACTATTGGAGGAAGGAGAGCAAAGTTTGTACAGAGAGTTCACTTGGTGGGATTACAAAATCTCTGCATATAAATCAAGGCTTGCAGACTGTAGGCGAAGTGATGATGTGTTTATAAGGGATGAGTGGGTTGTTACGGTAGAACTGTTTCCAAGATCAGTCACCGACTTTTCATCGTAG

Protein sequence

MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSDELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQKSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFRMNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSFFINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLADCRRSDDVFIRDEWVVTVELFPRSVTDFSS

Homology

[Close]

BLAST of Moc03g25890 vs. NCBI nr
Match: XP_038893915.1 (gibberellin 2-beta-dioxygenase-like [Benincasa hispida])

HSP 1 Score: 619.8 bits (1597), Expect = 1.5e-173
Identity = 290/322 (90.06%), Postives = 307/322 (95.34%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVIDL DP+AKTHIVKACEDFGFFKV NHGVSD
Sbjct: 32  MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTHIVKACEDFGFFKVANHGVSD 91

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           +LI A+EAQA +FF LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTNPQLIS+
Sbjct: 92  DLIGALEAQALKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNPQLISE 151

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KS  IF+ENP+IFRC VEEYI AVK MACEVLE+MAEGL IEKSALSRLL+DERSDCCFR
Sbjct: 152 KSFSIFRENPEIFRCLVEEYILAVKEMACEVLEVMAEGLRIEKSALSRLLKDERSDCCFR 211

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WVSVPPD TSF
Sbjct: 212 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 271

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSRFSMIYFGGPPLNEKIAPLPSLLE+GEQSL
Sbjct: 272 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEDGEQSL 331

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRLADCR
Sbjct: 332 YREFTWWDYKNSAYKSRLADCR 353

[Close]

BLAST of Moc03g25890 vs. NCBI nr
Match: NP_001269061.1 (gibberellin 2-beta-dioxygenase-like [Cucumis sativus] >KGN65541.1 hypothetical protein Csa_019824 [Cucumis sativus] >CCD28487.1 GA 2-oxidase [Cucumis sativus])

HSP 1 Score: 614.0 bits (1582), Expect = 8.0e-172
Identity = 286/322 (88.82%), Postives = 306/322 (95.03%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVI+L DP+AK  IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKIQIVKACEDFGFFKLVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           ELIAA+E+Q  +FF LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ
Sbjct: 61  ELIAALESQTLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KS+ IF ENP+IFRC VEEYI A+K MAC+VLE+MAEGL IEKSALS+LL+DERSDCCFR
Sbjct: 121 KSISIFHENPEIFRCLVEEYISAMKEMACQVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSRFSMIYFGGPPLNEKIAPLPSLLEEGE+SL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEKSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRLADCR
Sbjct: 301 YREFTWWDYKSSAYKSRLADCR 322

[Close]

BLAST of Moc03g25890 vs. NCBI nr
Match: XP_008444314.1 (PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo] >XP_008444315.1 PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo] >KAA0053981.1 gibberellin 2-beta-dioxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 608.6 bits (1568), Expect = 3.4e-170
Identity = 285/322 (88.51%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVI+  DP+AKT IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINPKDPEAKTQIVKACEDFGFFKLVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           ELIAA+E++  +FF LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNT PQLISQ
Sbjct: 61  ELIAALESETLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTKPQLISQ 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KS+ IF ENPDIFR  VEEYI A+K MACEVLE+MAEGL IEKSALS+LL+DERSDCCFR
Sbjct: 121 KSISIFHENPDIFRSLVEEYISAMKEMACEVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRL DCR
Sbjct: 301 YREFTWWDYKNSAYKSRLGDCR 322

[Close]

BLAST of Moc03g25890 vs. NCBI nr
Match: XP_023001443.1 (gibberellin 2-beta-dioxygenase-like [Cucurbita maxima] >CBL59328.1 Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 605.1 bits (1559), Expect = 3.7e-169
Identity = 283/322 (87.89%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVIDL DP+AKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
            LI  +EAQA +FF+LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTN +LIS+
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KSLFI +ENP+IFRCAVEEYI AVK MACEVLE MA GL IEKSALSRLL+DERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRLADCR
Sbjct: 301 YREFTWWDYKTSAYKSRLADCR 322

[Close]

BLAST of Moc03g25890 vs. NCBI nr
Match: XP_023542402.1 (gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 602.4 bits (1552), Expect = 2.4e-168
Identity = 285/322 (88.51%), Postives = 299/322 (92.86%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCK TA+F GIPVIDLSDP+AKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           +LI A+EAQA +FF LPQ EKEKAGP DPFGYGSKRIGPNGDVGWIEYLLLNTN +LIS+
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KSLFI +ENP+IFRCAVEEYI AVK MACEVLE MAEGLGIEK  LSRLL+DERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRF SVKHKVLADP K RFSMIYFGGPPLNEKIAPL SLLEEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASLLEEGEQRL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           Y+EFTWWDYK SAYKSRLA CR
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACR 322

[Close]

BLAST of Moc03g25890 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 474.6 bits (1220), Expect = 1.0e-132
Identity = 227/323 (70.28%), Postives = 268/323 (82.97%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVV SQ   + F  ++  K T +F GIPV+DL+ PDAK  IV AC DFGFFK+VNHGV  
Sbjct: 1   MVVLSQPALNQFFLLKPFKSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHGVPL 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           EL+A +E +A RFF+  Q EK++AGPPDPFGYGSKRIGPNGDVGW+EYLLLNTNP +IS 
Sbjct: 61  ELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDVISP 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGI-EKSALSRLLRDERSDCCF 180
           KSL IF+ENP  FR  VE YI AVK M   VLE+MAEGLGI +++ LSRLL+DE+SD CF
Sbjct: 121 KSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSDSCF 180

Query: 181 RMNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTS 240
           R+NHYPPCPE++AL+ RNL+GFGEHTDPQIISVLRSN+T+GL ICL DG+WVSVPPDQTS
Sbjct: 181 RLNHYPPCPEVQALN-RNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPDQTS 240

Query: 241 FFINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQS 300
           FFIN+GD+LQVMTNGRFKSVKH+VLAD  KSR SMIYFGGP L+E IAPLPS++ +GE+ 
Sbjct: 241 FFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKGEEC 300

Query: 301 LYREFTWWDYKISAYKSRLADCR 323
           LY+EFTW +YK +AY SRLAD R
Sbjct: 301 LYKEFTWCEYKKAAYTSRLADNR 322

[Close]

BLAST of Moc03g25890 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.9e-115
Identity = 205/327 (62.69%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MVVQSQLVF--DHFAPIRSCKRTAVF--EGIPVIDLSDPDAKTHIVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   V     IPV++L+DP+AKT IVKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQ 120
           GV  EL+  +E +A  FF LPQ  K +AGPP+P+GYG+KRIGPNGDVGWIEYLLLN NPQ
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISQKSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIE-KSALSRLLRDERS 180
           L S K+  +F++ P IFR +VEEY+  +K ++ +VLEM+AE LGIE +  LS++LRDE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRMNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPP 240
           D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DGSWV+VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQTSFFINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEE 300
           D +SFFIN+GD+LQVMTNGRFKSVKH+VLAD  +SR SMIYFGGPPL++KIAPLP L+ E
Sbjct: 241 DHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPE 300

Query: 301 GEQSLYREFTWWDYKISAYKSRLADCR 323
            +  LY+EFTW  YK SAYKS+L D R
Sbjct: 301 QDDWLYKEFTWSQYKSSAYKSKLGDYR 325

[Close]

BLAST of Moc03g25890 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 414.5 bits (1064), Expect = 1.2e-114
Identity = 198/323 (61.30%), Postives = 255/323 (78.95%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MV+ S+   + +  +R+         IP++DLS PDAKT IVKACEDFGFFKV+NHG+  
Sbjct: 1   MVLLSKPTSEQYTYVRNNMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHGIPL 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           + I+ +E++A +FF LPQ EKEKAGP +PFGYG+KRIG NGD+GWIEYLLL TN      
Sbjct: 61  DAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTN----QD 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIE-KSALSRLLRDERSDCCF 180
            +  ++ E+ D FR  +++Y  A++ MACE+L++MAEGL I+ K+  S+L+ D++SDC F
Sbjct: 121 HNFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLF 180

Query: 181 RMNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTS 240
           R+NHYP CPEL A++G NLIGFGEHTDPQIIS+LRSNNT+G  I LRDGSW+SVPPD +S
Sbjct: 181 RVNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSS 240

Query: 241 FFINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQS 300
           FFIN+GDSLQVMTNGRFKSV+H+VLA+ +  R SMIYF GPPL+EKIAPLPSL+ +G++S
Sbjct: 241 FFINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPSLM-KGKES 300

Query: 301 LYREFTWWDYKISAYKSRLADCR 323
           LY+EFTW++YK S Y SRLAD R
Sbjct: 301 LYKEFTWFEYKSSTYGSRLADNR 317

[Close]

BLAST of Moc03g25890 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 377.9 bits (969), Expect = 1.3e-103
Identity = 185/306 (60.46%), Postives = 228/306 (74.51%), Query Frame = 0


Query: 18  CKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSDELIAAIEAQARRFFELP 77
           CK   V   IPVIDL+D DAKT IVKACE+FGFFKV+NHGV  +L+  +E +A  FF L 
Sbjct: 20  CKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALH 79

Query: 78  QMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQKSLFIFQENPDIFRCAV 137
              K+KAGPPDPFGYG+KRIGPNGD+GW+EY+LLN N  L S K+  IF+  P IFR AV
Sbjct: 80  HSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAV 139

Query: 138 EEYIGAVKGMACEVLEMMAEGLGIE-KSALSRLLRDERSDCCFRMNHYPPCPELEALSGR 197
           EEYI  +K M+ + LEM+ E L IE K  LSRL++ + SD C RMNHY   PE E    +
Sbjct: 140 EEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVK 199

Query: 198 NLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSFFINIGDSLQVMTNGRF 257
             IGFGEHTDPQ+IS+LRSN+T GL IC++DG+WV V PD +SFF+ +GD+LQVMTNGRF
Sbjct: 200 EEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRF 259

Query: 258 KSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKS 317
           KSVKH+V+ +  +SR SMIYF GPPL+EKIAPL  L+ + +  LY EFTW  YK+SAYK+
Sbjct: 260 KSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKT 319

Query: 318 RLADCR 323
           +L D R
Sbjct: 320 KLGDYR 320

[Close]

BLAST of Moc03g25890 vs. ExPASy Swiss-Prot
Match: Q8S0S6 (Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2OX3 PE=1 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 1.5e-101
Identity = 182/314 (57.96%), Postives = 229/314 (72.93%), Query Frame = 0


Query: 10  DHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSDELIAAIEAQ 69
           DH   +RS     VF G+PV+DL  P A   +V ACE +GFFKVVNHGV+ + +   E++
Sbjct: 11  DHIPLLRSPDPGDVFSGVPVVDLGSPGAARAVVDACERYGFFKVVNHGVATDTMDKAESE 70

Query: 70  ARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQKSLFIFQEN 129
           A RFF   Q +K+++GP  PFGYGSKRIG NGD+GW+EYLLL  +   ++         +
Sbjct: 71  AVRFFSQTQPDKDRSGPAYPFGYGSKRIGFNGDMGWLEYLLLALDDASLADACTV---PS 130

Query: 130 PDIFRCAVEEYIGAVKGMACEVLEMMAEGLGI-EKSALSRLLRDERSDCCFRMNHYPPCP 189
             +FR A+ EYI  V+ +A  V+E M+EGLGI +  ALS L+  E SD  FR+NHYPPC 
Sbjct: 131 CAVFRAALNEYISGVRKVAVRVMEAMSEGLGIAQADALSALVTAEGSDQVFRVNHYPPCR 190

Query: 190 ELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSFFINIGDSL 249
            L+ L G ++ GFGEHTDPQ++SVLRSN T+GL I LRDG WVSVP D+ SFF+N+GDSL
Sbjct: 191 ALQGL-GCSVTGFGEHTDPQLVSVLRSNGTSGLQIALRDGQWVSVPSDRDSFFVNVGDSL 250

Query: 250 QVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWD 309
           QV+TNGRFKSVKH+V+A+ LKSR S IYFGGPPL ++IAPLP LL EGEQSLY+EFTW +
Sbjct: 251 QVLTNGRFKSVKHRVVANSLKSRVSFIYFGGPPLAQRIAPLPQLLGEGEQSLYKEFTWDE 310

Query: 310 YKISAYKSRLADCR 323
           YK +AYKSRL D R
Sbjct: 311 YKKAAYKSRLGDNR 320

[Close]

BLAST of Moc03g25890 vs. ExPASy TrEMBL
Match: S0BE08 (GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1)

HSP 1 Score: 614.0 bits (1582), Expect = 3.9e-172
Identity = 286/322 (88.82%), Postives = 306/322 (95.03%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVI+L DP+AK  IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINLKDPEAKIQIVKACEDFGFFKLVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           ELIAA+E+Q  +FF LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ
Sbjct: 61  ELIAALESQTLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KS+ IF ENP+IFRC VEEYI A+K MAC+VLE+MAEGL IEKSALS+LL+DERSDCCFR
Sbjct: 121 KSISIFHENPEIFRCLVEEYISAMKEMACQVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSRFSMIYFGGPPLNEKIAPLPSLLEEGE+SL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEKSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRLADCR
Sbjct: 301 YREFTWWDYKSSAYKSRLADCR 322

[Close]

BLAST of Moc03g25890 vs. ExPASy TrEMBL
Match: A0A1S3BA53 (gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103487682 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 285/322 (88.51%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVI+  DP+AKT IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINPKDPEAKTQIVKACEDFGFFKLVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           ELIAA+E++  +FF LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNT PQLISQ
Sbjct: 61  ELIAALESETLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTKPQLISQ 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KS+ IF ENPDIFR  VEEYI A+K MACEVLE+MAEGL IEKSALS+LL+DERSDCCFR
Sbjct: 121 KSISIFHENPDIFRSLVEEYISAMKEMACEVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRL DCR
Sbjct: 301 YREFTWWDYKNSAYKSRLGDCR 322

[Close]

BLAST of Moc03g25890 vs. ExPASy TrEMBL
Match: A0A5A7UKB4 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00270 PE=3 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 1.6e-170
Identity = 285/322 (88.51%), Postives = 303/322 (94.10%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVI+  DP+AKT IVKACEDFGFFK+VNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVINPKDPEAKTQIVKACEDFGFFKLVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           ELIAA+E++  +FF LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNT PQLISQ
Sbjct: 61  ELIAALESETLKFFHLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTKPQLISQ 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KS+ IF ENPDIFR  VEEYI A+K MACEVLE+MAEGL IEKSALS+LL+DERSDCCFR
Sbjct: 121 KSISIFHENPDIFRSLVEEYISAMKEMACEVLELMAEGLKIEKSALSKLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDG+WVSVPPD TSF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGNWVSVPPDHTSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRL DCR
Sbjct: 301 YREFTWWDYKNSAYKSRLGDCR 322

[Close]

BLAST of Moc03g25890 vs. ExPASy TrEMBL
Match: I0J1I9 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1)

HSP 1 Score: 605.1 bits (1559), Expect = 1.8e-169
Identity = 283/322 (87.89%), Postives = 302/322 (93.79%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCKRTA+F GIPVIDL DP+AKT IVKACEDFGFFKV+NHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKRTALFTGIPVIDLKDPEAKTQIVKACEDFGFFKVINHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
            LI  +EAQA +FF+LPQ EKEKAGPPDPFGYGSKRIGPNGDVGWIEY+LLNTN +LIS+
Sbjct: 61  HLIGDLEAQALKFFQLPQSEKEKAGPPDPFGYGSKRIGPNGDVGWIEYVLLNTNHELISE 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KSLFI +ENP+IFRCAVEEYI AVK MACEVLE MA GL IEKSALSRLL+DERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKEMACEVLEAMALGLRIEKSALSRLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVL+SNNT GLHICLRDG+WVSVPPD  SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLKSNNTAGLHICLRDGNWVSVPPDNASF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRFKSVKHKVLADP+KSR SMIYFGGPPLNEKIAPLPSL+EEGEQSL
Sbjct: 241 FINVGDSLQVMTNGRFKSVKHKVLADPIKSRLSMIYFGGPPLNEKIAPLPSLMEEGEQSL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           YREFTWWDYK SAYKSRLADCR
Sbjct: 301 YREFTWWDYKTSAYKSRLADCR 322

[Close]

BLAST of Moc03g25890 vs. ExPASy TrEMBL
Match: A0A6J1K680 (gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490445 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-168
Identity = 284/322 (88.20%), Postives = 299/322 (92.86%), Query Frame = 0


Query: 1   MVVQSQLVFDHFAPIRSCKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSD 60
           MVVQSQLVFDHFAPI+SCK TA+F GIPVIDLSDP+AKTHIVKACEDFGFFKVVNHGVSD
Sbjct: 1   MVVQSQLVFDHFAPIKSCKHTALFTGIPVIDLSDPEAKTHIVKACEDFGFFKVVNHGVSD 60

Query: 61  ELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQ 120
           +LI A+EAQA +FF LPQ EKEKAGP DPFGYGSKRIGPNGDVGWIEYLLLNTN +LIS+
Sbjct: 61  DLIGALEAQALKFFHLPQSEKEKAGPADPFGYGSKRIGPNGDVGWIEYLLLNTNHELISE 120

Query: 121 KSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIEKSALSRLLRDERSDCCFR 180
           KSLFI +ENP+IFRCAVEEYI AVK MACEVLE MAEGLGIEK  LSRLL+DERSDCCFR
Sbjct: 121 KSLFILRENPEIFRCAVEEYILAVKKMACEVLEAMAEGLGIEKHDLSRLLKDERSDCCFR 180

Query: 181 MNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSF 240
           +NHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWV VPPDQ SF
Sbjct: 181 LNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVPVPPDQRSF 240

Query: 241 FINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSL 300
           FIN+GDSLQVMTNGRF SVKHKVLADP K RFSMIYFGGPPLNEKIAPL S+LEEGEQ L
Sbjct: 241 FINVGDSLQVMTNGRFNSVKHKVLADPTKPRFSMIYFGGPPLNEKIAPLASILEEGEQRL 300

Query: 301 YREFTWWDYKISAYKSRLADCR 323
           Y+EFTWWDYK SAYKSRLA CR
Sbjct: 301 YKEFTWWDYKNSAYKSRLAACR 322

[Close]

BLAST of Moc03g25890 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 417.2 bits (1071), Expect = 1.3e-116
Identity = 205/327 (62.69%), Postives = 252/327 (77.06%), Query Frame = 0


Query: 1   MVVQSQLVF--DHFAPIRSCKRTAVF--EGIPVIDLSDPDAKTHIVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   V     IPV++L+DP+AKT IVKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQ 120
           GV  EL+  +E +A  FF LPQ  K +AGPP+P+GYG+KRIGPNGDVGWIEYLLLN NPQ
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISQKSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIE-KSALSRLLRDERS 180
           L S K+  +F++ P IFR +VEEY+  +K ++ +VLEM+AE LGIE +  LS++LRDE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRMNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPP 240
           D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DGSWV+VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQTSFFINIGDSLQVMTNGRFKSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEE 300
           D +SFFIN+GD+LQVMTNGRFKSVKH+VLAD  +SR SMIYFGGPPL++KIAPLP L+ E
Sbjct: 241 DHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVPE 300

Query: 301 GEQSLYREFTWWDYKISAYKSRLADCR 323
            +  LY+EFTW  YK SAYKS+L D R
Sbjct: 301 QDDWLYKEFTWSQYKSSAYKSKLGDYR 325

[Close]

BLAST of Moc03g25890 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 377.9 bits (969), Expect = 9.0e-105
Identity = 185/306 (60.46%), Postives = 228/306 (74.51%), Query Frame = 0


Query: 18  CKRTAVFEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSDELIAAIEAQARRFFELP 77
           CK   V   IPVIDL+D DAKT IVKACE+FGFFKV+NHGV  +L+  +E +A  FF L 
Sbjct: 20  CKPRPVL--IPVIDLTDSDAKTQIVKACEEFGFFKVINHGVRPDLLTQLEQEAINFFALH 79

Query: 78  QMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQKSLFIFQENPDIFRCAV 137
              K+KAGPPDPFGYG+KRIGPNGD+GW+EY+LLN N  L S K+  IF+  P IFR AV
Sbjct: 80  HSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLCLESHKTTAIFRHTPAIFREAV 139

Query: 138 EEYIGAVKGMACEVLEMMAEGLGIE-KSALSRLLRDERSDCCFRMNHYPPCPELEALSGR 197
           EEYI  +K M+ + LEM+ E L IE K  LSRL++ + SD C RMNHY   PE E    +
Sbjct: 140 EEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKESDSCLRMNHY---PEKEETPVK 199

Query: 198 NLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSFFINIGDSLQVMTNGRF 257
             IGFGEHTDPQ+IS+LRSN+T GL IC++DG+WV V PD +SFF+ +GD+LQVMTNGRF
Sbjct: 200 EEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTPDHSSFFVLVGDTLQVMTNGRF 259

Query: 258 KSVKHKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKS 317
           KSVKH+V+ +  +SR SMIYF GPPL+EKIAPL  L+ + +  LY EFTW  YK+SAYK+
Sbjct: 260 KSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPKQDDCLYNEFTWSQYKLSAYKT 319

Query: 318 RLADCR 323
           +L D R
Sbjct: 320 KLGDYR 320

[Close]

BLAST of Moc03g25890 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 362.5 bits (929), Expect = 3.9e-100
Identity = 175/302 (57.95%), Postives = 223/302 (73.84%), Query Frame = 0


Query: 24  FEGIPVIDLSDPDAKTHIVKACEDFGFFKVVNHGVSDELIAAIEAQARRFFELPQMEKEK 83
           F  IPVID+SDP++K  +VKACEDFGFFKV+NHGVS EL++ +E +   FF LP+ EK +
Sbjct: 15  FSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKSEKTQ 74

Query: 84  AGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQKSLFIFQENPDIFRCAVEEYIGA 143
                PFGYG+ +IG NGDVGW+EYLL+N N    S        ++P  FR A+EEY  +
Sbjct: 75  VA-GYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEEYTTS 134

Query: 144 VKGMACEVLEMMAEGLGIE-KSALSRLLRDERSDCCFRMNHYPPCP--ELEALSGRNLIG 203
           V+ M  +VLE + +GLGI+ ++ LS+L+ D+ +D   R+NHYPPCP    +   G+N+IG
Sbjct: 135 VRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGKNVIG 194

Query: 204 FGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSFFINIGDSLQVMTNGRFKSVK 263
           FGEHTDPQIISVLRSNNT+GL I L DGSW+SVPPD TSFF N+GDSLQVMTNGRFKSV+
Sbjct: 195 FGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRFKSVR 254

Query: 264 HKVLADPLKSRFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRLAD 323
           H+VLA+  KSR SMIYF GP L ++IAPL  L++  ++ LY EFTW +YK S Y SRL+D
Sbjct: 255 HRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNSRLSD 314

[Close]

BLAST of Moc03g25890 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 310.8 bits (795), Expect = 1.4e-84
Identity = 154/255 (60.39%), Postives = 193/255 (75.69%), Query Frame = 0


Query: 1   MVVQSQLVF--DHFAPIRSCKRTAVF--EGIPVIDLSDPDAKTHIVKACEDFGFFKVVNH 60
           MVV  Q V   +H + I + K   V     IPV++L+DP+AKT IVKACE+FGFFKVVNH
Sbjct: 1   MVVLPQPVTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVNH 60

Query: 61  GVSDELIAAIEAQARRFFELPQMEKEKAGPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQ 120
           GV  EL+  +E +A  FF LPQ  K +AGPP+P+GYG+KRIGPNGDVGWIEYLLLN NPQ
Sbjct: 61  GVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANPQ 120

Query: 121 LISQKSLFIFQENPDIFRCAVEEYIGAVKGMACEVLEMMAEGLGIE-KSALSRLLRDERS 180
           L S K+  +F++ P IFR +VEEY+  +K ++ +VLEM+AE LGIE +  LS++LRDE+S
Sbjct: 121 LSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEKS 180

Query: 181 DCCFRMNHYPPCPELEALSGRNLIGFGEHTDPQIISVLRSNNTTGLHICLRDGSWVSVPP 240
           D C R+NHYP   E      +  +GFGEHTDPQIISVLRSNNT GL IC++DGSWV+VPP
Sbjct: 181 DSCLRLNHYPAAEEEAEKMVK--VGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVPP 240

Query: 241 DQTSFFINIGDSLQV 251
           D +SFFIN+GD+LQV
Sbjct: 241 DHSSFFINVGDALQV 253

[Close]

BLAST of Moc03g25890 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 303.1 bits (775), Expect = 2.8e-82
Identity = 150/296 (50.68%), Postives = 191/296 (64.53%), Query Frame = 0


Query: 28  PVIDLSDPD---AKTHIVKACEDFGFFKVVNHGVSDELIAAIEAQARRFFELPQMEKEKA 87
           PVID S  D       IVKACE  GFFKV+NHGV  E+I   E +   FF  P+ +K +A
Sbjct: 25  PVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEHEGEEFFNKPESDKLRA 84

Query: 88  GPPDPFGYGSKRIGPNGDVGWIEYLLLNTNPQLISQKSLFIFQENPDIFRCAVEEYIGAV 147
           GP  PFGYG K IG NGD+G +EYLLL+ NP  ++ KS  I  ++P  F  A  +YI  V
Sbjct: 85  GPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHDDPFKFSSATNDYIRTV 144

Query: 148 KGMACEVLEMMAEGL-GIEKSALSRLLRDERSDCCFRMNHYPPCPELEALSGRNLIGFGE 207
           + +ACE++++  E L G + S +S L+RD RSD   R+NHYPP P   ALSG   IGFGE
Sbjct: 145 RDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPAP--YALSGVGQIGFGE 204

Query: 208 HTDPQIISVLRSNNTTGLHICLRDGSWVSVPPDQTSFFINIGDSLQVMTNGRFKSVKHKV 267
           H+DPQI++VLRSN+  GL IC RDG W+ +P D T FF+ +GD LQ +TNGRF SV+H+V
Sbjct: 205 HSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDCLQALTNGRFTSVRHRV 264

Query: 268 LADPLKS-RFSMIYFGGPPLNEKIAPLPSLLEEGEQSLYREFTWWDYKISAYKSRL 319
           LA+  K  R S +YF  PPL  KI+PLP ++       Y  FTW DYK + Y  RL
Sbjct: 265 LANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTWGDYKKATYSLRL 318

The following BLAST results are available for this feature:

BLAST of Moc03g25890 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893915.1	1.5e-173	90.06	gibberellin 2-beta-dioxygenase-like [Benincasa hispida]	[more]
NP_001269061.1	8.0e-172	88.82	gibberellin 2-beta-dioxygenase-like [Cucumis sativus] >KGN65541.1 hypothetical p...	[more]
XP_008444314.1	3.4e-170	88.51	PREDICTED: gibberellin 2-beta-dioxygenase-like [Cucumis melo] >XP_008444315.1 PR...	[more]
XP_023001443.1	3.7e-169	87.89	gibberellin 2-beta-dioxygenase-like [Cucurbita maxima] >CBL59328.1 Gibberellin 2...	[more]
XP_023542402.1	2.4e-168	88.51	gibberellin 2-beta-dioxygenase-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Moc03g25890 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	1.0e-132	70.28	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9XFR9	1.9e-115	62.69	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q9SQ80	1.2e-114	61.30	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
O64692	1.3e-103	60.46	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]
Q8S0S6	1.5e-101	57.96	Gibberellin 2-beta-dioxygenase 3 OS=Oryza sativa subsp. japonica OX=39947 GN=GA2...	[more]

BLAST of Moc03g25890 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
S0BE08	3.9e-172	88.82	GA 2-oxidase OS=Cucumis sativus OX=3659 GN=GA2ox3 PE=2 SV=1	[more]
A0A1S3BA53	1.6e-170	88.51	gibberellin 2-beta-dioxygenase-like OS=Cucumis melo OX=3656 GN=LOC103487682 PE=3...	[more]
A0A5A7UKB4	1.6e-170	88.51	Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6...	[more]
I0J1I9	1.8e-169	87.89	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox3 PE=2 SV=1	[more]
A0A6J1K680	2.0e-168	88.20	gibberellin 2-beta-dioxygenase-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO...	[more]

BLAST of Moc03g25890 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	1.3e-116	62.69	gibberellin 2-oxidase	[more]
AT2G34555.1	9.0e-105	60.46	gibberellin 2-oxidase 3	[more]
AT1G78440.1	3.9e-100	57.95	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT1G30040.2	1.4e-84	60.39	gibberellin 2-oxidase	[more]
AT1G02400.1	2.8e-82	50.68	gibberellin 2-oxidase 6	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 204..225 score: 27.69 coord: 41..58 score: 56.06 coord: 240..266 score: 29.29
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 7..322
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 7..322
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 16..319
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 8..319 e-value: 8.5E-85 score: 287.0
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 27..113 e-value: 3.7E-18 score: 66.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..278 e-value: 1.3E-25 score: 89.8
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 174..280 score: 11.08087

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g25890.1	Moc03g25890.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0045487	gibberellin catabolic process
biological_process	GO:0009416	response to light stimulus
molecular_function	GO:0052634	C-19 gibberellin 2-beta-dioxygenase activity
molecular_function	GO:0046872	metal ion binding