Moc03g25860 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g25860 vs. NCBI nr
Match: XP_022139981.1 (cullin-4 [Momordica charantia])

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 718/718 (100.00%), Postives = 718/718 (100.00%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA
Sbjct: 66  MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 125

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ
Sbjct: 126 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 185

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 186 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 245

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 246 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 305

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD
Sbjct: 306 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 365

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL
Sbjct: 366 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 425

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 426 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 485

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 486 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 545

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 546 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 605

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 606 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 665

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL
Sbjct: 666 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 725

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 726 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 783

BLAST of Moc03g25860 vs. NCBI nr
Match: XP_022954928.1 (cullin-4-like [Cucurbita moschata])

HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 694/718 (96.66%), Postives = 705/718 (98.19%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSPLIGTSRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECE+HISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECELHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTP+VCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPNVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMY LISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYNLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAFCNTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFCNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKG+KELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGRKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVED+DSFVFNE FTAPL
Sbjct: 681 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDHDSFVFNEVFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. NCBI nr
Match: KAG6583898.1 (Cullin-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1361.3 bits (3522), Expect = 0.0e+00
Identity = 695/718 (96.80%), Postives = 704/718 (98.05%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMV SL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVSSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTAPL
Sbjct: 681 AEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. NCBI nr
Match: XP_023541784.1 (cullin-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1360.5 bits (3520), Expect = 0.0e+00
Identity = 695/718 (96.80%), Postives = 704/718 (98.05%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDS LIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSHLIGTSRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECE+HISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECELHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMY LISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYNLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAFCNTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFCNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVED+DSFVFNE FTAPL
Sbjct: 681 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDHDSFVFNEVFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. NCBI nr
Match: XP_023519705.1 (cullin-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1360.1 bits (3519), Expect = 0.0e+00
Identity = 694/718 (96.66%), Postives = 704/718 (98.05%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV SL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVSSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTAPL
Sbjct: 681 AEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. ExPASy Swiss-Prot
Match: Q8LGH4 (Cullin-4 OS=Arabidopsis thaliana OX=3702 GN=CUL4 PE=1 SV=1)

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 598/702 (85.19%), Postives = 630/702 (89.74%), Query Frame = 0

Query: 17  DSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWAKLKSAICAIFLKQPNS 76
           D    A NLSRKKAT PQP KK VIKL KAKPTLP NFEE+TW KL+SAI AIFLK+  S
Sbjct: 54  DPTPAAANLSRKKATLPQPTKKFVIKLNKAKPTLPTNFEENTWEKLQSAIRAIFLKKKIS 113

Query: 77  CDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQ 136
            DLE LYQAV++LCLHK+ G LY +IEKECE HISAALQSLVGQ+ DL VFL  VEKCWQ
Sbjct: 114 FDLESLYQAVDNLCLHKLDGKLYDQIEKECEEHISAALQSLVGQNTDLTVFLSRVEKCWQ 173

Query: 137 DFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMI 196
           DFCDQMLMIR IAL LDR YV Q P+V SLW+MGLQLFRKHLSL+ EVE +TV GLL MI
Sbjct: 174 DFCDQMLMIRSIALTLDRKYVIQNPNVRSLWEMGLQLFRKHLSLAPEVEQRTVKGLLSMI 233

Query: 197 EKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYL 256
           EKERL EAVNRTLL+HLLKMFTALGIY ESFEKPFLE TSEFYAAEGMKYMQ SDV EYL
Sbjct: 234 EKERLAEAVNRTLLSHLLKMFTALGIYMESFEKPFLEGTSEFYAAEGMKYMQQSDVPEYL 293

Query: 257 KHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDLQ 316
           KH E RL EENERC+LY+D  TRKPLI T ERQLLERHI  +L+KGFT LMDG R EDLQ
Sbjct: 294 KHVEGRLHEENERCILYIDAVTRKPLITTVERQLLERHILVVLEKGFTTLMDGRRTEDLQ 353

Query: 317 RMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFS 376
           RM TL SRVNALESLRQALSSY+R+TGQ IVMD+EKDKDMV SLL+FKASLD IWEESF 
Sbjct: 354 RMQTLFSRVNALESLRQALSSYVRKTGQKIVMDEEKDKDMVQSLLDFKASLDIIWEESFY 413

Query: 377 KNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFR 436
           KNE+F NTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELE  L+KVLVLFR
Sbjct: 414 KNESFGNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELESVLEKVLVLFR 473

Query: 437 FIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELS 496
           FIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELS
Sbjct: 474 FIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELS 533

Query: 497 KEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLS 556
           KEINESFKQSSQARTKLP GIEMSVHVLTTGYWPTYPPMDV+LPHELNVYQDIFKEFYLS
Sbjct: 534 KEINESFKQSSQARTKLPSGIEMSVHVLTTGYWPTYPPMDVKLPHELNVYQDIFKEFYLS 593

Query: 557 KYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIE 616
           KYSGRRLMW NSLGHCVLKA+F KGKKELAVSLFQ VVLMLFNDA KLSF+DI++ST IE
Sbjct: 594 KYSGRRLMWQNSLGHCVLKADFSKGKKELAVSLFQAVVLMLFNDAMKLSFEDIKDSTSIE 653

Query: 617 DKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEE 676
           DKELRRTLQSLACGKVRVLQK PKGRDVED D F FN+ F APLYR+KVNAIQMKETVEE
Sbjct: 654 DKELRRTLQSLACGKVRVLQKNPKGRDVEDGDEFEFNDEFAAPLYRIKVNAIQMKETVEE 713

Query: 677 NTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           NTSTTERVFQDRQYQ+DAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 714 NTSTTERVFQDRQYQIDAAIVRIMKTRKVLSHTLLITELFQQ 755

BLAST of Moc03g25860 vs. ExPASy Swiss-Prot
Match: Q13620 (Cullin-4B OS=Homo sapiens OX=9606 GN=CUL4B PE=1 SV=4)

HSP 1 Score: 785.4 bits (2027), Expect = 6.0e-226
Identity = 401/698 (57.45%), Postives = 518/698 (74.21%), Query Frame = 0

Query: 22  ATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEK 81
           +T +S    + P  AKKLVIK  K KP LP N+ ++TW KLK A+ AI        +LE+
Sbjct: 180 STTVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEE 239

Query: 82  LYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQ 141
           LYQAV +LC +K+  NLY+++ + CE HI A +      S D V+FLK +++CWQ+ C Q
Sbjct: 240 LYQAVENLCSYKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQ 299

Query: 142 MLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERL 201
           M+MIR I L+LDRTYV Q   + S+WDMGL+LFR H+    +V++KT+ G+L +IE+ER 
Sbjct: 300 MIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQNKTIDGILLLIERERN 359

Query: 202 GEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEE 261
           GEA++R+LL  LL M + L IY +SFE+ FLE T+  YAAEG K MQ  +V EYL H  +
Sbjct: 360 GEAIDRSLLRSLLSMLSDLQIYQDSFEQRFLEETNRLYAAEGQKLMQEREVPEYLHHVNK 419

Query: 262 RLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDLQRMYTL 321
           RL+EE +R + YLD +T+K LIAT E+QLL  H++AIL KG   L+D NR++DL  +Y L
Sbjct: 420 RLEEEADRLITYLDQTTQKSLIATVEKQLLGEHLTAILQKGLNNLLDENRIQDLSLLYQL 479

Query: 322 ISRV-NALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFSKNEA 381
            SRV   ++ L Q    YI+  G  IV++ EKDK MV  LL+FK  +D I +  F KNE 
Sbjct: 480 FSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDICFLKNEK 539

Query: 382 FCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQG 441
           F N +K+AFE  IN R N+PAELIAK++D KLRAGNK  ++EELE  LDK++++FRFI G
Sbjct: 540 FINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYG 599

Query: 442 KDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEIN 501
           KDVFEAFYKKDLAKRLL+GKSAS+DAEKSM+SKLK ECG+ FT+KLEGMFKD+ELSK+I 
Sbjct: 600 KDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIM 659

Query: 502 ESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSG 561
             FKQ  Q    +P  IE++V++LT GYWPTY PM+V LP E+   Q+IFK FYL K+SG
Sbjct: 660 IQFKQYMQ-NQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSG 719

Query: 562 RRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKEL 621
           R+L W ++LGHCVLKAEF +GKKEL VSLFQT+VL++FN+ E+ S ++I+++TGIED EL
Sbjct: 720 RKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTLVLLMFNEGEEFSLEEIKQATGIEDGEL 779

Query: 622 RRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEENTST 681
           RRTLQSLACGK RVL K PKG+D+ED D F+ N+ F   L+R+K+N IQMKETVEE  ST
Sbjct: 780 RRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQAST 839

Query: 682 TERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           TERVFQDRQYQ+DAAIVRIMK RK LSH LL++E++ Q
Sbjct: 840 TERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQ 876

BLAST of Moc03g25860 vs. ExPASy Swiss-Prot
Match: A2A432 (Cullin-4B OS=Mus musculus OX=10090 GN=Cul4b PE=1 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.7e-225
Identity = 400/698 (57.31%), Postives = 516/698 (73.93%), Query Frame = 0

Query: 22  ATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEK 81
           +T +S    + P  AKKLVIK  K KP LP N+ ++TW KLK A+ AI        +LE+
Sbjct: 237 STAVSSFANSKPGSAKKLVIKNFKDKPKLPENYTDETWQKLKEAVEAIQNSTSIKYNLEE 296

Query: 82  LYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQ 141
           LYQAV +LC HK+  NLY+++ + CE HI A +      S D V+FLK +++CWQ+ C Q
Sbjct: 297 LYQAVENLCSHKISANLYKQLRQICEDHIKAQIHQFREDSLDSVLFLKKIDRCWQNHCRQ 356

Query: 142 MLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERL 201
           M+MIR I L+LDRTYV Q   + S+WDMGL+LFR H+    +V+ KT+ G+L +IE+ER 
Sbjct: 357 MIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRAHIISDQKVQTKTIDGILLLIERERN 416

Query: 202 GEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEE 261
           GEA++R+LL  LL M + L IY +SFE+ FL+ T+  YAAEG K MQ  +V EYL H  +
Sbjct: 417 GEAIDRSLLRSLLSMLSDLQIYQDSFEQQFLQETNRLYAAEGQKLMQEREVPEYLHHVNK 476

Query: 262 RLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDLQRMYTL 321
           RL+EE +R + YLD +T+K LIA+ E+QLL  H++AIL KG   L+D NR++DL  +Y L
Sbjct: 477 RLEEEADRLITYLDQTTQKSLIASVEKQLLGEHLTAILQKGLNSLLDENRIQDLSLLYQL 536

Query: 322 ISRV-NALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFSKNEA 381
            SRV   ++ L Q    YI+  G  IV++ EKDK MV  LL+FK  +D I +  F KNE 
Sbjct: 537 FSRVRGGVQVLLQQWIEYIKAFGSTIVINPEKDKTMVQELLDFKDKVDHIIDTCFLKNEK 596

Query: 382 FCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQG 441
           F N +K+AFE  IN R N+PAELIAK++D KLRAGNK  ++EELE  LDK++++FRFI G
Sbjct: 597 FINAMKEAFETFINKRPNKPAELIAKYVDSKLRAGNKEATDEELEKMLDKIMIIFRFIYG 656

Query: 442 KDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEIN 501
           KDVFEAFYKKDLAKRLL+GKSAS+DAEKSM+SKLK ECG+ FT+KLEGMFKD+ELSK+I 
Sbjct: 657 KDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHECGAAFTSKLEGMFKDMELSKDIM 716

Query: 502 ESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSG 561
             FKQ  Q    +P  IE++V++LT GYWPTY PM+V LP E+   Q+IFK FYL K+SG
Sbjct: 717 IQFKQYMQ-NQNVPGNIELTVNILTMGYWPTYVPMEVHLPPEMVKLQEIFKTFYLGKHSG 776

Query: 562 RRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKEL 621
           R+L W ++LGHCVLKAEF +GKKEL VSLFQT+VL++FN+ E+ S ++I+ +TGIED EL
Sbjct: 777 RKLQWQSTLGHCVLKAEFKEGKKELQVSLFQTMVLLMFNEGEEFSLEEIKHATGIEDGEL 836

Query: 622 RRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEENTST 681
           RRTLQSLACGK RVL K PKG+D+ED D F+ N+ F   L+R+K+N IQMKETVEE  ST
Sbjct: 837 RRTLQSLACGKARVLAKNPKGKDIEDGDKFICNDDFKHKLFRIKINQIQMKETVEEQAST 896

Query: 682 TERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           TERVFQDRQYQ+DAAIVRIMK RK LSH LL++E++ Q
Sbjct: 897 TERVFQDRQYQIDAAIVRIMKMRKTLSHNLLVSEVYNQ 933

BLAST of Moc03g25860 vs. ExPASy Swiss-Prot
Match: Q13619 (Cullin-4A OS=Homo sapiens OX=9606 GN=CUL4A PE=1 SV=3)

HSP 1 Score: 766.5 bits (1978), Expect = 2.9e-220
Identity = 403/724 (55.66%), Postives = 518/724 (71.55%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQP-----AKKLVIKLLKAKPTLPANFE 60
           MA +   K   S L+G +  + T  +   A P +P     +KKLVIK  + +P LP N+ 
Sbjct: 1   MADEAPRKGSFSALVGRTNGL-TKPAALAAAPAKPGGAGGSKKLVIKNFRDRPRLPDNYT 60

Query: 61  EDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQ 120
           +DTW KL  A+ A+        +LE+LYQAV +LC HK+   LY+++ + CE H+ A + 
Sbjct: 61  QDTWRKLHEAVRAVQSSTSIRYNLEELYQAVENLCSHKVSPMLYKQLRQACEDHVQAQIL 120

Query: 121 SLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFR 180
                S D V+FLK +  CWQD C QM+MIR I L+LDRTYV Q  ++ S+WDMGL+LFR
Sbjct: 121 PFREDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSTLPSIWDMGLELFR 180

Query: 181 KHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYT 240
            H+     V+ KT+ G+L +IE+ER GEAV+R+LL  LL M + L +Y +SFE  FLE T
Sbjct: 181 THIISDKMVQSKTIDGILLLIERERSGEAVDRSLLRSLLGMLSDLQVYKDSFELKFLEET 240

Query: 241 SEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHI 300
           +  YAAEG + MQ  +V EYL H  +RL+EE +R + YLD ST+KPLIA  E+QLL  H+
Sbjct: 241 NCLYAAEGQRLMQEREVPEYLNHVSKRLEEEGDRVITYLDHSTQKPLIACVEKQLLGEHL 300

Query: 301 SAILDKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSSYIRRTGQNIVMDDEKDK 360
           +AIL KG   L+D NR+ DL +MY L SRV    ++L Q  S YI+  G  IV++ EKDK
Sbjct: 301 TAILQKGLDHLLDENRVPDLAQMYQLFSRVRGGQQALLQHWSEYIKTFGTAIVINPEKDK 360

Query: 361 DMVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRA 420
           DMV  LL+FK  +D + E  F KNE F N +K++FE  IN R N+PAELIAK +D KLRA
Sbjct: 361 DMVQDLLDFKDKVDHVIEVCFQKNERFVNLMKESFETFINKRPNKPAELIAKHVDSKLRA 420

Query: 421 GNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKL 480
           GNK  ++EELE TLDK+++LFRFI GKDVFEAFYKKDLAKRLL+GKSAS+DAEKSM+SKL
Sbjct: 421 GNKEATDEELERTLDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKL 480

Query: 481 KTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPP 540
           K ECG+ FT+KLEGMFKD+ELSK+I   FKQ  Q ++     I+++V++LT GYWPTY P
Sbjct: 481 KHECGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQSD-SGPIDLTVNILTMGYWPTYTP 540

Query: 541 MDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVV 600
           M+V L  E+   Q++FK FYL K+SGR+L W  +LGH VLKAEF +GKKE  VSLFQT+V
Sbjct: 541 MEVHLTPEMIKLQEVFKAFYLGKHSGRKLQWQTTLGHAVLKAEFKEGKKEFQVSLFQTLV 600

Query: 601 LMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNE 660
           L++FN+ +  SF++I+ +TGIED ELRRTLQSLACGK RVL K PKG++VED D F+FN 
Sbjct: 601 LLMFNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNG 660

Query: 661 GFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITE 719
            F   L+R+K+N IQMKETVEE  STTERVFQDRQYQ+DAAIVRIMK RK L H LL++E
Sbjct: 661 EFKHKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSE 720

BLAST of Moc03g25860 vs. ExPASy Swiss-Prot
Match: Q3TCH7 (Cullin-4A OS=Mus musculus OX=10090 GN=Cul4a PE=1 SV=1)

HSP 1 Score: 761.5 bits (1965), Expect = 9.2e-219
Identity = 400/721 (55.48%), Postives = 511/721 (70.87%), Query Frame = 0

Query: 10  DDSPLIGDSRAV---ATNLSRKKATPPQPAK--------KLVIKLLKAKPTLPANFEEDT 69
           D+ P  G   A+      L++  A    PAK        KLVIK  + +P LP N+ +DT
Sbjct: 3   DEGPRKGSVSALMGRTNGLTKPAALAGGPAKPGGTGGSRKLVIKNFRDRPRLPDNYTQDT 62

Query: 70  WAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLV 129
           W KL  A+ AI        +LE+LYQAV +LC HK+   LY+++ + CE H+ A +    
Sbjct: 63  WRKLHEAVKAIQSSTSIRYNLEELYQAVENLCSHKVSPTLYKQLRQVCEDHVQAQILPFR 122

Query: 130 GQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHL 189
             S D V+FLK +  CWQD C QM+MIR I L+LDRTYV Q   + S+WDMGL+LFR H+
Sbjct: 123 EDSLDSVLFLKKINTCWQDHCRQMIMIRSIFLFLDRTYVLQNSMLPSIWDMGLELFRNHI 182

Query: 190 SLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEF 249
                V+ KT+ G+L +I +ER GEAV+R+LL  LL M + L +Y +SFE  FLE T+  
Sbjct: 183 ISDRMVQSKTIDGILLLIGRERSGEAVDRSLLRSLLSMLSDLQVYKDSFELKFLEETNCL 242

Query: 250 YAAEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAI 309
           YAAEG + MQ  +V EYL H  +RL+EE +R + YLD ST+KPLIA  E+QLL  H++AI
Sbjct: 243 YAAEGQRLMQDREVPEYLNHVSKRLEEEADRVITYLDHSTQKPLIACVEKQLLGEHLTAI 302

Query: 310 LDKGFTLLMDGNRMEDLQRMYTLISRV-NALESLRQALSSYIRRTGQNIVMDDEKDKDMV 369
           L KG   L+D NR+ DL +MY L SRV     +L Q  S YI+  G  IV++ EKDKDMV
Sbjct: 303 LQKGLEHLLDENRVPDLTQMYQLFSRVKGGQHALLQHWSEYIKTFGTTIVINPEKDKDMV 362

Query: 370 PSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNK 429
             LL+FK  +D + E  F +NE F N +K++FE  IN R N+PAELIAK +D KLRAGNK
Sbjct: 363 QDLLDFKDKVDHVVEVCFQRNERFINLMKESFETFINKRPNKPAELIAKHVDSKLRAGNK 422

Query: 430 GTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTE 489
             ++EELE  LDK+++LFRFI GKDVFEAFYKKDLAKRLL+GKSAS+DAEKSM+SKLK E
Sbjct: 423 EATDEELERILDKIMILFRFIHGKDVFEAFYKKDLAKRLLVGKSASVDAEKSMLSKLKHE 482

Query: 490 CGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDV 549
           CG+ FT+KLEGMFKD+ELSK+I   FKQ  Q ++  P  I+++V++LT GYWPTY PM+V
Sbjct: 483 CGAAFTSKLEGMFKDMELSKDIMVHFKQHMQNQS-APGPIDLTVNILTMGYWPTYTPMEV 542

Query: 550 RLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLML 609
            LP E+   Q++FK FYL K+SGR+L W  +LGH VLKA+F +GKKE  VSLFQT+VL++
Sbjct: 543 HLPPEMVRLQEVFKTFYLGKHSGRKLQWQTTLGHAVLKADFKEGKKEFQVSLFQTLVLLM 602

Query: 610 FNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFT 669
           FN+ +  SF++I+ +TGIED ELRRTLQSLACGK RVL K PKG++VED D F+FN  F 
Sbjct: 603 FNEGDGFSFEEIKMATGIEDSELRRTLQSLACGKARVLIKSPKGKEVEDGDKFIFNADFK 662

Query: 670 APLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQ 719
             L+R+K+N IQMKETVEE  STTERVFQDRQYQ+DAAIVRIMK RK L H LL++EL+ 
Sbjct: 663 HKLFRIKINQIQMKETVEEQVSTTERVFQDRQYQIDAAIVRIMKMRKTLGHNLLVSELYN 722

BLAST of Moc03g25860 vs. ExPASy TrEMBL
Match: A0A6J1CEB0 (cullin-4 OS=Momordica charantia OX=3673 GN=LOC111010756 PE=3 SV=1)

HSP 1 Score: 1410.2 bits (3649), Expect = 0.0e+00
Identity = 718/718 (100.00%), Postives = 718/718 (100.00%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA
Sbjct: 66  MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 125

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ
Sbjct: 126 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 185

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 186 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 245

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 246 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 305

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD
Sbjct: 306 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 365

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL
Sbjct: 366 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 425

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 426 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 485

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 486 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 545

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 546 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 605

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 606 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 665

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL
Sbjct: 666 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 725

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 726 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 783

BLAST of Moc03g25860 vs. ExPASy TrEMBL
Match: A0A6J1GSH7 (cullin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111457039 PE=3 SV=1)

HSP 1 Score: 1361.7 bits (3523), Expect = 0.0e+00
Identity = 694/718 (96.66%), Postives = 705/718 (98.19%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSPLIGTSRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECE+HISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECELHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTP+VCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPNVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMY LISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYNLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAFCNTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFCNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKG+KELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGRKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVED+DSFVFNE FTAPL
Sbjct: 681 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDHDSFVFNEVFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. ExPASy TrEMBL
Match: A0A6J1EK69 (cullin-4-like OS=Cucurbita moschata OX=3662 GN=LOC111434080 PE=3 SV=1)

HSP 1 Score: 1359.0 bits (3516), Expect = 0.0e+00
Identity = 693/718 (96.52%), Postives = 704/718 (98.05%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV SL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVSSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASID+EKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDSEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTAPL
Sbjct: 681 AEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. ExPASy TrEMBL
Match: A0A6J1KQD7 (cullin-4-like OS=Cucurbita maxima OX=3661 GN=LOC111495542 PE=3 SV=1)

HSP 1 Score: 1358.6 bits (3515), Expect = 0.0e+00
Identity = 693/718 (96.52%), Postives = 703/718 (97.91%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDLKPDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLKPDDSPLIGASRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECEVHIS ALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECEVHISVALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEA+NRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAINRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ SDVSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSDVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMYTLISRVNALESLRQALSSYIR+TGQNIVMDDEKDKDMV SL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYTLISRVNALESLRQALSSYIRKTGQNIVMDDEKDKDMVSSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAF NTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFSNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLS QDIRESTGIEDKELRRTLQSLACGKVRVLQK+PKGRDVEDNDSF+FNEGFTAPL
Sbjct: 681 AEKLSLQDIRESTGIEDKELRRTLQSLACGKVRVLQKMPKGRDVEDNDSFMFNEGFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. ExPASy TrEMBL
Match: A0A6J1K265 (cullin-4-like OS=Cucurbita maxima OX=3661 GN=LOC111490376 PE=3 SV=1)

HSP 1 Score: 1358.6 bits (3515), Expect = 0.0e+00
Identity = 694/718 (96.66%), Postives = 703/718 (97.91%), Query Frame = 0

Query: 1   MALDEDLKPDDSPLIGDSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWA 60
           MALDEDL PDDSPLIG SRAVATNLSRKKAT PQPAKKLVIKL+KAKPTLPANFEEDTWA
Sbjct: 81  MALDEDLNPDDSPLIGTSRAVATNLSRKKATLPQPAKKLVIKLVKAKPTLPANFEEDTWA 140

Query: 61  KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQ 120
           KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLY+RIEKECE+HISAALQSLVGQ
Sbjct: 141 KLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHKMGGNLYRRIEKECELHISAALQSLVGQ 200

Query: 121 SPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 180
           SPDLVVFL +VEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL
Sbjct: 201 SPDLVVFLAYVEKCWQDFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSL 260

Query: 181 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 240
           SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA
Sbjct: 261 SSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYA 320

Query: 241 AEGMKYMQLSDVSEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILD 300
           AEGMK+MQ S VSEYLKHAE RLQ E +RCL YLD+STRKPLIAT ERQLLERHISAILD
Sbjct: 321 AEGMKHMQQSYVSEYLKHAEGRLQAEQDRCLHYLDSSTRKPLIATTERQLLERHISAILD 380

Query: 301 KGFTLLMDGNRMEDLQRMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 360
           KGFTLLMDGNRM DL RMY LISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL
Sbjct: 381 KGFTLLMDGNRMGDLLRMYNLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSL 440

Query: 361 LEFKASLDTIWEESFSKNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 420
           LEFKASLDTIWEESFSKNEAFCNTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTS
Sbjct: 441 LEFKASLDTIWEESFSKNEAFCNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTS 500

Query: 421 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 480
           EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS
Sbjct: 501 EEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGS 560

Query: 481 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 540
           QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP
Sbjct: 561 QFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLP 620

Query: 541 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 600
           HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND
Sbjct: 621 HELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFND 680

Query: 601 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPL 660
           AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVED+DSFVFNE FTAPL
Sbjct: 681 AEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDHDSFVFNEVFTAPL 740

Query: 661 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 741 YRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 798

BLAST of Moc03g25860 vs. TAIR 10
Match: AT5G46210.1 (cullin4 )

HSP 1 Score: 1163.7 bits (3009), Expect = 0.0e+00
Identity = 598/702 (85.19%), Postives = 630/702 (89.74%), Query Frame = 0

Query: 17  DSRAVATNLSRKKATPPQPAKKLVIKLLKAKPTLPANFEEDTWAKLKSAICAIFLKQPNS 76
           D    A NLSRKKAT PQP KK VIKL KAKPTLP NFEE+TW KL+SAI AIFLK+  S
Sbjct: 54  DPTPAAANLSRKKATLPQPTKKFVIKLNKAKPTLPTNFEENTWEKLQSAIRAIFLKKKIS 113

Query: 77  CDLEKLYQAVNDLCLHKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQ 136
            DLE LYQAV++LCLHK+ G LY +IEKECE HISAALQSLVGQ+ DL VFL  VEKCWQ
Sbjct: 114 FDLESLYQAVDNLCLHKLDGKLYDQIEKECEEHISAALQSLVGQNTDLTVFLSRVEKCWQ 173

Query: 137 DFCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMI 196
           DFCDQMLMIR IAL LDR YV Q P+V SLW+MGLQLFRKHLSL+ EVE +TV GLL MI
Sbjct: 174 DFCDQMLMIRSIALTLDRKYVIQNPNVRSLWEMGLQLFRKHLSLAPEVEQRTVKGLLSMI 233

Query: 197 EKERLGEAVNRTLLNHLLKMFTALGIYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYL 256
           EKERL EAVNRTLL+HLLKMFTALGIY ESFEKPFLE TSEFYAAEGMKYMQ SDV EYL
Sbjct: 234 EKERLAEAVNRTLLSHLLKMFTALGIYMESFEKPFLEGTSEFYAAEGMKYMQQSDVPEYL 293

Query: 257 KHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDKGFTLLMDGNRMEDLQ 316
           KH E RL EENERC+LY+D  TRKPLI T ERQLLERHI  +L+KGFT LMDG R EDLQ
Sbjct: 294 KHVEGRLHEENERCILYIDAVTRKPLITTVERQLLERHILVVLEKGFTTLMDGRRTEDLQ 353

Query: 317 RMYTLISRVNALESLRQALSSYIRRTGQNIVMDDEKDKDMVPSLLEFKASLDTIWEESFS 376
           RM TL SRVNALESLRQALSSY+R+TGQ IVMD+EKDKDMV SLL+FKASLD IWEESF 
Sbjct: 354 RMQTLFSRVNALESLRQALSSYVRKTGQKIVMDEEKDKDMVQSLLDFKASLDIIWEESFY 413

Query: 377 KNEAFCNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFR 436
           KNE+F NTIKD+FEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELE  L+KVLVLFR
Sbjct: 414 KNESFGNTIKDSFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELESVLEKVLVLFR 473

Query: 437 FIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELS 496
           FIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELS
Sbjct: 474 FIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELS 533

Query: 497 KEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLS 556
           KEINESFKQSSQARTKLP GIEMSVHVLTTGYWPTYPPMDV+LPHELNVYQDIFKEFYLS
Sbjct: 534 KEINESFKQSSQARTKLPSGIEMSVHVLTTGYWPTYPPMDVKLPHELNVYQDIFKEFYLS 593

Query: 557 KYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIE 616
           KYSGRRLMW NSLGHCVLKA+F KGKKELAVSLFQ VVLMLFNDA KLSF+DI++ST IE
Sbjct: 594 KYSGRRLMWQNSLGHCVLKADFSKGKKELAVSLFQAVVLMLFNDAMKLSFEDIKDSTSIE 653

Query: 617 DKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKVNAIQMKETVEE 676
           DKELRRTLQSLACGKVRVLQK PKGRDVED D F FN+ F APLYR+KVNAIQMKETVEE
Sbjct: 654 DKELRRTLQSLACGKVRVLQKNPKGRDVEDGDEFEFNDEFAAPLYRIKVNAIQMKETVEE 713

Query: 677 NTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           NTSTTERVFQDRQYQ+DAAIVRIMKTRKVLSHTLLITELFQQ
Sbjct: 714 NTSTTERVFQDRQYQIDAAIVRIMKTRKVLSHTLLITELFQQ 755

BLAST of Moc03g25860 vs. TAIR 10
Match: AT1G69670.1 (cullin 3B )

HSP 1 Score: 483.8 bits (1244), Expect = 2.6e-136
Identity = 271/697 (38.88%), Postives = 426/697 (61.12%), Query Frame = 0

Query: 34  QPAKKLVIKLLKAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHK 93
           Q  +   I+  K +  +   + + TW  L+ AI  I+    +    E+LY+   ++ LHK
Sbjct: 4   QKKRNFQIEAFKQRVVVDPKYADKTWKILEHAIHEIYNHNASGLSFEELYRNAYNMVLHK 63

Query: 94  MGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIALYLD 153
            G  LY  +      H+    +S+  +      FL+ + + W D    + MIR I +Y+D
Sbjct: 64  YGDKLYTGLVTTMTFHLKEICKSI--EEAQGGAFLELLNRKWNDHNKALQMIRDILMYMD 123

Query: 154 RTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLLNHL 213
           RTYV  T     + ++GL L+R ++  SS+++ + +  LL ++ KER GE ++R L+ ++
Sbjct: 124 RTYVSTTKKT-HVHELGLHLWRDNVVYSSKIQTRLLNTLLDLVHKERTGEVIDRVLMRNV 183

Query: 214 LKMFTALG--IYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENERCL 273
           +KMF  LG  +Y + FEKPFLE ++EFY  E M++++  D  EYLK AE+ L EE ER +
Sbjct: 184 IKMFMDLGESVYQDDFEKPFLEASAEFYKVESMEFIESCDCGEYLKKAEKPLVEEVERVV 243

Query: 274 LYLDTSTRKPLIATAERQLLERHISAIL---DKGFTLLMDGNRMEDLQRMYTLISRV-NA 333
            YLD  +   + +  ER+++  H+  ++   + G   ++  ++ ED+ RMY+L  RV N 
Sbjct: 244 NYLDAKSEAKITSVVEREMIANHVQRLVHMENSGLVNMLLNDKYEDMGRMYSLFRRVANG 303

Query: 334 LESLRQALSSYIRRTGQNIVMDDEKDKD---MVPSLLEFKASLDTIWEESFSKNEAFCNT 393
           L ++R  ++ ++R  G+ +V D EK KD    V  LL+ +   D I   +F+ ++ F N 
Sbjct: 304 LVTVRDVMTLHLREMGKQLVTDPEKSKDPVEFVQRLLDERDKYDRIINMAFNNDKTFQNA 363

Query: 394 IKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGKDVF 453
           +  +FE+ +NL    P E I+ F+D+KLR G KG  EE+++  LDKV++LFR++Q KDVF
Sbjct: 364 LNSSFEYFVNLNTRSP-EFISLFVDDKLRKGLKGVGEEDVDLILDKVMMLFRYLQEKDVF 423

Query: 454 EAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINESFK 513
           E +YK+ LAKRLL GK+ S DAE+++I KLKTECG QFT+KLEGMF D++ S +    F 
Sbjct: 424 EKYYKQHLAKRLLSGKTVSDDAERNLIVKLKTECGYQFTSKLEGMFTDMKTSHDTLLGFY 483

Query: 514 QSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGRRLM 573
            S     +L  G  + V VLTTG WPT P +   LP E++V  + F+ +YL  ++GRRL 
Sbjct: 484 NS---HPELSEGPTLVVQVLTTGSWPTQPTIQCNLPAEVSVLCEKFRSYYLGTHTGRRLS 543

Query: 574 WHNSLGHCVLKAEFPKGKK-ELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKELRRT 633
           W  ++G   +KA F KG+K EL VS FQ  VLMLFN++++LS+++I ++T I   +L+R 
Sbjct: 544 WQTNMGTADIKAVFGKGQKHELNVSTFQMCVLMLFNNSDRLSYKEIEQATEIPTPDLKRC 603

Query: 634 LQSLACGKVR-VLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKV-NAIQMKETVEENTSTT 693
           LQS+AC K + VL+K P  +++ + D FV N+ F +  Y++K+   +  KET  E   T 
Sbjct: 604 LQSMACVKGKNVLRKEPMSKEIAEEDWFVVNDRFASKFYKVKIGTVVAQKETEPEKQETR 663

Query: 694 ERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
           +RV +DR+ Q++AAIVRIMK+R+VL H  +I E+ +Q
Sbjct: 664 QRVEEDRKPQIEAAIVRIMKSRRVLDHNNIIAEVTKQ 693

BLAST of Moc03g25860 vs. TAIR 10
Match: AT1G26830.1 (cullin 3 )

HSP 1 Score: 481.1 bits (1237), Expect = 1.7e-135
Identity = 274/700 (39.14%), Postives = 429/700 (61.29%), Query Frame = 0

Query: 34  QPAKKLVIKLLKAKPTLPANFEEDTWAKLKSAICAIFLKQPNSCDLEKLYQAVNDLCLHK 93
           Q  +   I+  K +  +   + + TW  L+ AI  I+ +  +    E+LY+   ++ LHK
Sbjct: 4   QKKRNFQIEAFKHRVVVDPKYADKTWQILERAIHQIYNQDASGLSFEELYRNAYNMVLHK 63

Query: 94  MGGNLYQRIEKECEVHI---SAALQSLVGQSPDLVVFLKFVEKCWQDFCDQMLMIRGIAL 153
            G  LY         H+   S  +++  G S     FL+ + K W +    + MIR I +
Sbjct: 64  FGEKLYTGFIATMTSHLKEKSKLIEAAQGGS-----FLEELNKKWNEHNKALEMIRDILM 123

Query: 154 YLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIEKERLGEAVNRTLL 213
           Y+DRTY++ T     +  MGL L+R ++   +++  + +  LL +++KER+GE ++R L+
Sbjct: 124 YMDRTYIESTKKT-HVHPMGLNLWRDNVVHFTKIHTRLLNTLLDLVQKERIGEVIDRGLM 183

Query: 214 NHLLKMFTALG--IYSESFEKPFLEYTSEFYAAEGMKYMQLSDVSEYLKHAEERLQEENE 273
            +++KMF  LG  +Y E FEKPFL+ +SEFY  E  ++++  D  +YLK +E+RL EE E
Sbjct: 184 RNVIKMFMDLGESVYQEDFEKPFLDASSEFYKVESQEFIESCDCGDYLKKSEKRLTEEIE 243

Query: 274 RCLLYLDTSTRKPLIATAERQLLERHISAIL---DKGFTLLMDGNRMEDLQRMYTLISRV 333
           R   YLD  + + + +  E++++  H+  ++   + G   ++  ++ EDL RMY L  RV
Sbjct: 244 RVAHYLDAKSEEKITSVVEKEMIANHMQRLVHMENSGLVNMLLNDKYEDLGRMYNLFRRV 303

Query: 334 -NALESLRQALSSYIRRTGQNIVMDDEKDKD---MVPSLLEFKASLDTIWEESFSKNEAF 393
            N L ++R  ++S++R  G+ +V D EK KD    V  LL+ +   D I   +F  ++ F
Sbjct: 304 TNGLVTVRDVMTSHLREMGKQLVTDPEKSKDPVEFVQRLLDERDKYDKIINTAFGNDKTF 363

Query: 394 CNTIKDAFEHLINLRQNRPAELIAKFLDEKLRAGNKGTSEEELEGTLDKVLVLFRFIQGK 453
            N +  +FE+ INL    P E I+ F+D+KLR G KG ++ ++E  LDKV++LFR++Q K
Sbjct: 364 QNALNSSFEYFINLNARSP-EFISLFVDDKLRKGLKGITDVDVEVILDKVMMLFRYLQEK 423

Query: 454 DVFEAFYKKDLAKRLLLGKSASIDAEKSMISKLKTECGSQFTNKLEGMFKDIELSKEINE 513
           DVFE +YK+ LAKRLL GK+ S DAE+S+I KLKTECG QFT+KLEGMF D++ S++   
Sbjct: 424 DVFEKYYKQHLAKRLLSGKTVSDDAERSLIVKLKTECGYQFTSKLEGMFTDMKTSEDTMR 483

Query: 514 SFKQSSQARTKLPMGIEMSVHVLTTGYWPTYPPMDVRLPHELNVYQDIFKEFYLSKYSGR 573
            F  S     +L  G  + V VLTTG WPT P +   LP E++V  + F+ +YL  ++GR
Sbjct: 484 GFYGS---HPELSEGPTLIVQVLTTGSWPTQPAVPCNLPAEVSVLCEKFRSYYLGTHTGR 543

Query: 574 RLMWHNSLGHCVLKAEFPKGKK-ELAVSLFQTVVLMLFNDAEKLSFQDIRESTGIEDKEL 633
           RL W  ++G   +KA F KG+K EL VS FQ  VLMLFN++++LS+++I ++T I   +L
Sbjct: 544 RLSWQTNMGTADIKAIFGKGQKHELNVSTFQMCVLMLFNNSDRLSYKEIEQATEIPAADL 603

Query: 634 RRTLQSLACGKVR-VLQKIPKGRDVEDNDSFVFNEGFTAPLYRLKV-NAIQMKETVEENT 693
           +R LQSLAC K + V++K P  +D+ + D FV N+ FT+  Y++K+   +  KET  E  
Sbjct: 604 KRCLQSLACVKGKNVIKKEPMSKDIGEEDLFVVNDKFTSKFYKVKIGTVVAQKETEPEKQ 663

Query: 694 STTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLITELFQQ 719
            T +RV +DR+ Q++AAIVRIMK+RK+L H  +I E+ +Q
Sbjct: 664 ETRQRVEEDRKPQIEAAIVRIMKSRKILDHNNIIAEVTKQ 693

BLAST of Moc03g25860 vs. TAIR 10
Match: AT4G02570.1 (cullin 1 )

HSP 1 Score: 349.0 bits (894), Expect = 1.0e-95
Identity = 217/666 (32.58%), Postives = 360/666 (54.05%), Query Frame = 0

Query: 82  LYQAVNDLCL----HKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQD 141
           LY  + ++C     H     LY +  +  E +I++ +   + +  D    L+ + K W +
Sbjct: 42  LYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHD-EFMLRELFKRWSN 101

Query: 142 FCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIE 201
               +  +     YLDR ++ +  S+  L ++GL  FR    + +E+  K    ++ +++
Sbjct: 102 HKVMVRWLSRFFYYLDRYFIARR-SLPPLNEVGLTCFRD--LVYNELHSKVKQAVIALVD 161

Query: 202 KERLGEAVNRTLLNHLLKMFTALGI-----YSESFEKPFLEYTSEFYAAEGMKYMQLSDV 261
           KER GE ++R LL ++L ++  +G+     Y E FE   L+ TS +Y+ +   ++Q    
Sbjct: 162 KEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSC 221

Query: 262 SEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDK---GFTLLMDG 321
            +Y+  +EE L++E ER   YL +S+   L+   + +LL    S +L+K   G   L+  
Sbjct: 222 PDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRD 281

Query: 322 NRMEDLQRMYTLISRV-NALESLRQALSSYIRRTGQNIVMDDE-------------KDKD 381
           ++++DL RMY L  ++   LE +      ++   G  +V   E             +++ 
Sbjct: 282 DKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQV 341

Query: 382 MVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLIN--LRQNRPAELIAKFLDEKL- 441
           ++  ++E          E F  +  F   +K+AFE   N  +  +  AEL+A F D  L 
Sbjct: 342 LIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILK 401

Query: 442 RAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMIS 501
           + G++  S+E +E TL+KV+ L  +I  KD+F  FY+K LA+RLL  +SA+ D E+S+++
Sbjct: 402 KGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILT 461

Query: 502 KLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTY 561
           KLK +CG QFT+K+EGM  D+ L++E   SF+    +      GI+++V VLTTG+WP+Y
Sbjct: 462 KLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSY 521

Query: 562 PPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQT 621
              D+ LP E+    ++FK FY +K   R+L W  SLG C +  +F +   EL VS +Q 
Sbjct: 522 KSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQA 581

Query: 622 VVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVF 681
            VL+LFN  +KLS+ +I     +  ++L R L SL+C K ++L K P  + V  ND+F F
Sbjct: 582 AVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEF 641

Query: 682 NEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLI 719
           N  FT  + R+K+        V+E     E V +DR+Y +DAAIVRIMK+RKVL H  L+
Sbjct: 642 NSKFTDRMRRIKIPL----PPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLV 699

BLAST of Moc03g25860 vs. TAIR 10
Match: AT4G02570.2 (cullin 1 )

HSP 1 Score: 349.0 bits (894), Expect = 1.0e-95
Identity = 217/666 (32.58%), Postives = 360/666 (54.05%), Query Frame = 0

Query: 82  LYQAVNDLCL----HKMGGNLYQRIEKECEVHISAALQSLVGQSPDLVVFLKFVEKCWQD 141
           LY  + ++C     H     LY +  +  E +I++ +   + +  D    L+ + K W +
Sbjct: 42  LYTTIYNMCTQKPPHDYSQQLYDKYREAFEEYINSTVLPALREKHD-EFMLRELFKRWSN 101

Query: 142 FCDQMLMIRGIALYLDRTYVKQTPSVCSLWDMGLQLFRKHLSLSSEVEHKTVTGLLRMIE 201
               +  +     YLDR ++ +  S+  L ++GL  FR    + +E+  K    ++ +++
Sbjct: 102 HKVMVRWLSRFFYYLDRYFIARR-SLPPLNEVGLTCFRD--LVYNELHSKVKQAVIALVD 161

Query: 202 KERLGEAVNRTLLNHLLKMFTALGI-----YSESFEKPFLEYTSEFYAAEGMKYMQLSDV 261
           KER GE ++R LL ++L ++  +G+     Y E FE   L+ TS +Y+ +   ++Q    
Sbjct: 162 KEREGEQIDRALLKNVLDIYVEIGMGQMERYEEDFESFMLQDTSSYYSRKASSWIQEDSC 221

Query: 262 SEYLKHAEERLQEENERCLLYLDTSTRKPLIATAERQLLERHISAILDK---GFTLLMDG 321
            +Y+  +EE L++E ER   YL +S+   L+   + +LL    S +L+K   G   L+  
Sbjct: 222 PDYMLKSEECLKKERERVAHYLHSSSEPKLVEKVQHELLVVFASQLLEKEHSGCRALLRD 281

Query: 322 NRMEDLQRMYTLISRV-NALESLRQALSSYIRRTGQNIVMDDE-------------KDKD 381
           ++++DL RMY L  ++   LE +      ++   G  +V   E             +++ 
Sbjct: 282 DKVDDLSRMYRLYHKILRGLEPVANIFKQHVTAEGNALVQQAEDTATNQVANTASVQEQV 341

Query: 382 MVPSLLEFKASLDTIWEESFSKNEAFCNTIKDAFEHLIN--LRQNRPAELIAKFLDEKL- 441
           ++  ++E          E F  +  F   +K+AFE   N  +  +  AEL+A F D  L 
Sbjct: 342 LIRKVIELHDKYMVYVTECFQNHTLFHKALKEAFEIFCNKTVAGSSSAELLATFCDNILK 401

Query: 442 RAGNKGTSEEELEGTLDKVLVLFRFIQGKDVFEAFYKKDLAKRLLLGKSASIDAEKSMIS 501
           + G++  S+E +E TL+KV+ L  +I  KD+F  FY+K LA+RLL  +SA+ D E+S+++
Sbjct: 402 KGGSEKLSDEAIEDTLEKVVKLLAYISDKDLFAEFYRKKLARRLLFDRSANDDHERSILT 461

Query: 502 KLKTECGSQFTNKLEGMFKDIELSKEINESFKQSSQARTKLPMGIEMSVHVLTTGYWPTY 561
           KLK +CG QFT+K+EGM  D+ L++E   SF+    +      GI+++V VLTTG+WP+Y
Sbjct: 462 KLKQQCGGQFTSKMEGMVTDLTLARENQNSFEDYLGSNPAANPGIDLTVTVLTTGFWPSY 521

Query: 562 PPMDVRLPHELNVYQDIFKEFYLSKYSGRRLMWHNSLGHCVLKAEFPKGKKELAVSLFQT 621
              D+ LP E+    ++FK FY +K   R+L W  SLG C +  +F +   EL VS +Q 
Sbjct: 522 KSFDINLPSEMIKCVEVFKGFYETKTKHRKLTWIYSLGTCHINGKFDQKAIELIVSTYQA 581

Query: 622 VVLMLFNDAEKLSFQDIRESTGIEDKELRRTLQSLACGKVRVLQKIPKGRDVEDNDSFVF 681
            VL+LFN  +KLS+ +I     +  ++L R L SL+C K ++L K P  + V  ND+F F
Sbjct: 582 AVLLLFNTTDKLSYTEILAQLNLSHEDLVRLLHSLSCAKYKILLKEPNTKTVSQNDAFEF 641

Query: 682 NEGFTAPLYRLKVNAIQMKETVEENTSTTERVFQDRQYQVDAAIVRIMKTRKVLSHTLLI 719
           N  FT  + R+K+        V+E     E V +DR+Y +DAAIVRIMK+RKVL H  L+
Sbjct: 642 NSKFTDRMRRIKIPL----PPVDERKKVVEDVDKDRRYAIDAAIVRIMKSRKVLGHQQLV 699