Moc03g25220 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g25220
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Pectinesterase
Location	chr3: 18119960 .. 18121753 (-)
RNA-Seq Expression	Moc03g25220
Synteny	Moc03g25220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTCTGCTCAGACCTTACGGTAAGGTCGACGAAACCGACCAGCTGAGGCTCGACGCTTCCCGGAGGACCCGGAAGAGGATTGCTGTCATTGTGCTGTCCTCTGTTGTTCTTGTTGCTGTTGTTGTTGCTGTTGTGTTTGGGACTAGAAATGGTTCTAAGGATGATGGCAATGGCAATGGCAATGGCAATTCTGCTCTTTCAAGTTCCATCAAAGCAGTTTGTGATGTTACTTTGTATCCGGAGACGTGTAACTATGCTTTCGGACCTTTGTCTAACTCGAGCCGCCTCGACCCGGGCCAGATCTGCAAGCTTTCGGTTCAACTGGCGCTTGGCGAACTGTCGCGAGCAGCCGACTATGTTTTCAAGCATGCCATTACCAATTCAACTGACAATAGAACCAAATTGGCTTTGGAAAGTTGCCATGAACTTTTGGACTTGGCATTGGATCATTTGAATATCTCACTCTCTTCTACTGAAATTACATTGCTGAAAGCTGTGGATGATCTTAAAACATGGCTAACTTCGGCTGCTACTTACCAGCAGACGTGCATTGAAGGCCTCGAGGAAGTCGATCCGACTTTGAGGAACAGCATTGCCAATTACTTGAAGAACTCTACTGAATTGACAAGCAATGGCCTTGCCATTGTGTCATTCTTTTCGAAAATTACGGATTCGCTCAATCTACGGCGGTTGATGAGTTATGAGAATCATCAGAGCAGTGCAGATTGGGCACGCCCGATACTCCGAAAGCTCGCTCAGCGTGATCTTAGGAAGCAGGCTGACATCGTTGTGGCGAAAGACGGTTCGGGGAAGTACAAGACAATCAGTGCTGCTCTCAAGGCAGTTCCTGACAAGAGCAAGAAGAGCACTGTGATTTATGTGAAGAAGGGAACTTACAAGGAGAATGTTGAAGTTGGGAAGAACAAATGGAATGTGGTGATGATTGGTGATGGAATGAATGCCACCACTGTAACTGCCAAACTCAATTTCATAGATGGGACTCCAACATTTTCATCTGCAACATTTGGTAAGAGGGAAAAACAACGGATTCTTCAGTCCGTTGGATAACAATTTCATTCTCTAATTTTCATGTATGTTTTCGTTTTTTGTGCAGCTGCAAAGGGCAAAGGCTTTATTGCCATAGACATGGGTTTCCAGAACATCGCTGGACCGAGCAAACACCAGGCGGTCGCCCTGATGTCAACCTCGGACCAATCGATCTTCTACCGCTGTAAAATGGATGCATACCAAGACACTCTCTACGCACACTCGAACCGTCAATTCTACCGCGAATGCACGGTCGAGGGCACGGTTGATTTCATCTTTGGTAACTCAGCAGTAGTTCTGCAGAACTGCACCATTTTGCCCAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAACCAAAACACTGGCATTTCCATACAAGACTCTTCCATCACACCATACGAGAACTTGAAAGCTACCGAAACGTACCTCGGTCGTCCGTGGAAGAACTACTCAACGACGGTATTCATGCAGAACTACCTCGGGAGCCTGATTCAGCCAACTGGGTGGCTGCCATGGGTAGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTCCAAAACTATGGCCCTGGTTCTTCCACAGCCAAAAGGGTCAAATGGAAGGGTGTGAGGAATATTGACAACAAAACTGCCAAAAAGTTCACAGTTTCATCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTGCCCTTCAAATCTGGCCTCTGA

mRNA sequence

ATGTCTCTGCTCAGACCTTACGGTAAGGTCGACGAAACCGACCAGCTGAGGCTCGACGCTTCCCGGAGGACCCGGAAGAGGATTGCTGTCATTGTGCTGTCCTCTGTTGTTCTTGTTGCTGTTGTTGTTGCTGTTGTGTTTGGGACTAGAAATGGTTCTAAGGATGATGGCAATGGCAATGGCAATGGCAATTCTGCTCTTTCAAGTTCCATCAAAGCAGTTTGTGATGTTACTTTGTATCCGGAGACGTGTAACTATGCTTTCGGACCTTTGTCTAACTCGAGCCGCCTCGACCCGGGCCAGATCTGCAAGCTTTCGGTTCAACTGGCGCTTGGCGAACTGTCGCGAGCAGCCGACTATGTTTTCAAGCATGCCATTACCAATTCAACTGACAATAGAACCAAATTGGCTTTGGAAAGTTGCCATGAACTTTTGGACTTGGCATTGGATCATTTGAATATCTCACTCTCTTCTACTGAAATTACATTGCTGAAAGCTGTGGATGATCTTAAAACATGGCTAACTTCGGCTGCTACTTACCAGCAGACGTGCATTGAAGGCCTCGAGGAAGTCGATCCGACTTTGAGGAACAGCATTGCCAATTACTTGAAGAACTCTACTGAATTGACAAGCAATGGCCTTGCCATTGTGTCATTCTTTTCGAAAATTACGGATTCGCTCAATCTACGGCGGTTGATGAGTTATGAGAATCATCAGAGCAGTGCAGATTGGGCACGCCCGATACTCCGAAAGCTCGCTCAGCGTGATCTTAGGAAGCAGGCTGACATCGTTGTGGCGAAAGACGGTTCGGGGAAGTACAAGACAATCAGTGCTGCTCTCAAGGCAGTTCCTGACAAGAGCAAGAAGAGCACTGTGATTTATGTGAAGAAGGGAACTTACAAGGAGAATGTTGAAGTTGGGAAGAACAAATGGAATGTGGTGATGATTGGTGATGGAATGAATGCCACCACTGTAACTGCCAAACTCAATTTCATAGATGGGACTCCAACATTTTCATCTGCAACATTTGCTGCAAAGGGCAAAGGCTTTATTGCCATAGACATGGGTTTCCAGAACATCGCTGGACCGAGCAAACACCAGGCGGTCGCCCTGATGTCAACCTCGGACCAATCGATCTTCTACCGCTGTAAAATGGATGCATACCAAGACACTCTCTACGCACACTCGAACCGTCAATTCTACCGCGAATGCACGGTCGAGGGCACGGTTGATTTCATCTTTGGTAACTCAGCAGTAGTTCTGCAGAACTGCACCATTTTGCCCAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAACCAAAACACTGGCATTTCCATACAAGACTCTTCCATCACACCATACGAGAACTTGAAAGCTACCGAAACGTACCTCGGTCGTCCGTGGAAGAACTACTCAACGACGGTATTCATGCAGAACTACCTCGGGAGCCTGATTCAGCCAACTGGGTGGCTGCCATGGGTAGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTCCAAAACTATGGCCCTGGTTCTTCCACAGCCAAAAGGGTCAAATGGAAGGGTGTGAGGAATATTGACAACAAAACTGCCAAAAAGTTCACAGTTTCATCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTGCCCTTCAAATCTGGCCTCTGA

Coding sequence (CDS)

ATGTCTCTGCTCAGACCTTACGGTAAGGTCGACGAAACCGACCAGCTGAGGCTCGACGCTTCCCGGAGGACCCGGAAGAGGATTGCTGTCATTGTGCTGTCCTCTGTTGTTCTTGTTGCTGTTGTTGTTGCTGTTGTGTTTGGGACTAGAAATGGTTCTAAGGATGATGGCAATGGCAATGGCAATGGCAATTCTGCTCTTTCAAGTTCCATCAAAGCAGTTTGTGATGTTACTTTGTATCCGGAGACGTGTAACTATGCTTTCGGACCTTTGTCTAACTCGAGCCGCCTCGACCCGGGCCAGATCTGCAAGCTTTCGGTTCAACTGGCGCTTGGCGAACTGTCGCGAGCAGCCGACTATGTTTTCAAGCATGCCATTACCAATTCAACTGACAATAGAACCAAATTGGCTTTGGAAAGTTGCCATGAACTTTTGGACTTGGCATTGGATCATTTGAATATCTCACTCTCTTCTACTGAAATTACATTGCTGAAAGCTGTGGATGATCTTAAAACATGGCTAACTTCGGCTGCTACTTACCAGCAGACGTGCATTGAAGGCCTCGAGGAAGTCGATCCGACTTTGAGGAACAGCATTGCCAATTACTTGAAGAACTCTACTGAATTGACAAGCAATGGCCTTGCCATTGTGTCATTCTTTTCGAAAATTACGGATTCGCTCAATCTACGGCGGTTGATGAGTTATGAGAATCATCAGAGCAGTGCAGATTGGGCACGCCCGATACTCCGAAAGCTCGCTCAGCGTGATCTTAGGAAGCAGGCTGACATCGTTGTGGCGAAAGACGGTTCGGGGAAGTACAAGACAATCAGTGCTGCTCTCAAGGCAGTTCCTGACAAGAGCAAGAAGAGCACTGTGATTTATGTGAAGAAGGGAACTTACAAGGAGAATGTTGAAGTTGGGAAGAACAAATGGAATGTGGTGATGATTGGTGATGGAATGAATGCCACCACTGTAACTGCCAAACTCAATTTCATAGATGGGACTCCAACATTTTCATCTGCAACATTTGCTGCAAAGGGCAAAGGCTTTATTGCCATAGACATGGGTTTCCAGAACATCGCTGGACCGAGCAAACACCAGGCGGTCGCCCTGATGTCAACCTCGGACCAATCGATCTTCTACCGCTGTAAAATGGATGCATACCAAGACACTCTCTACGCACACTCGAACCGTCAATTCTACCGCGAATGCACGGTCGAGGGCACGGTTGATTTCATCTTTGGTAACTCAGCAGTAGTTCTGCAGAACTGCACCATTTTGCCCAAGTTGCCTCTTTTAGGCCAAAAGAACACAATCACTGCTCAAGGCCGGTTTGATCCTAACCAAAACACTGGCATTTCCATACAAGACTCTTCCATCACACCATACGAGAACTTGAAAGCTACCGAAACGTACCTCGGTCGTCCGTGGAAGAACTACTCAACGACGGTATTCATGCAGAACTACCTCGGGAGCCTGATTCAGCCAACTGGGTGGCTGCCATGGGTAGGAACATCAGCACCAGACACCATATTCTATGCAGAGTTCCAAAACTATGGCCCTGGTTCTTCCACAGCCAAAAGGGTCAAATGGAAGGGTGTGAGGAATATTGACAACAAAACTGCCAAAAAGTTCACAGTTTCATCCTTCATTGATGGTAAAGATTGGATCTCAAAGGCACAAGTGCCCTTCAAATCTGGCCTCTGA

Protein sequence

MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTAKKFTVSSFIDGKDWISKAQVPFKSGL

Homology

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BLAST of Moc03g25220 vs. NCBI nr
Match: XP_022140040.1 (putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia])

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
           GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY
Sbjct: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPFKSGL
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568

[Close]

BLAST of Moc03g25220 vs. NCBI nr
Match: XP_038895610.1 (putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida])

HSP 1 Score: 971.8 bits (2511), Expect = 2.5e-279
Identity = 493/568 (86.80%), Postives = 527/568 (92.78%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLV VVVAVVFGTRN S ++ + N
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVGVVVAVVFGTRNASNNNDDSN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N + ALS+SIKAVCDVTLYP TC  AFGPL+NSS LDP QI KLSVQLALGELSR ADY
Sbjct: 61  -NQSGALSTSIKAVCDVTLYPVTCQNAFGPLANSSHLDPSQISKLSVQLALGELSRVADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           +F HAITNSTDN+T LAL++CHELLDLALDHLNISLSS++ITLLKAVDDLKTWLTSAATY
Sbjct: 121 IFYHAITNSTDNKTILALKNCHELLDLALDHLNISLSSSDITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GL EVDP L N IANYLKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYE+HQS
Sbjct: 181 QQTCIDGLTEVDPALANLIANYLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDHQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQRD+RKQADIVVAKD SGKYKTI+AAL+AVP+KSKK TVIYVKKGTY
Sbjct: 241 NGDWARPILRKLAQRDIRKQADIVVAKDSSGKYKTITAALEAVPNKSKKRTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEV KNKWNVV++GDGM AT VT  LN IDGTPTFS+ATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVVKNKWNVVIVGDGMTATIVTGSLNVIDGTPTFSTATFAAKGKGFIAIDMGFRNT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGP+KHQAVALMSTSDQSIFYRC+MDAYQDTLY+HSNRQFYREC V GTVDFIFGNSAVV
Sbjct: 361 AGPNKHQAVALMSTSDQSIFYRCQMDAYQDTLYSHSNRQFYRECKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQDSSI PYENLK TETYLGRPWKNY
Sbjct: 421 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQDSSIQPYENLKTTETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKW GV+NID K
Sbjct: 481 STTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWTGVKNIDIK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPFK+ +
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKASI 567

[Close]

BLAST of Moc03g25220 vs. NCBI nr
Match: XP_023000829.1 (probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima])

HSP 1 Score: 944.5 bits (2440), Expect = 4.2e-271
Identity = 480/568 (84.51%), Postives = 515/568 (90.67%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D   +
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKND--ES 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N ALS+SIKAVCD TLYP+TCN AFGPL+NSS LDPGQI K SVQLAL ELSR ADY
Sbjct: 61  DNHNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           + +HAITNSTDN+T LAL  CHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATY
Sbjct: 121 LLEHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYE+  S
Sbjct: 181 QQTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ ALKAV DKSKK TVIYVKKG Y
Sbjct: 241 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEV KNKWNVVMIGDGM +TTVT KLNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK T+TYLGRPWKNY
Sbjct: 421 LQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID K
Sbjct: 481 STTVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFNATL 566

[Close]

BLAST of Moc03g25220 vs. NCBI nr
Match: KAG6583848.1 (putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 941.0 bits (2431), Expect = 4.7e-270
Identity = 479/568 (84.33%), Postives = 514/568 (90.49%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D   +
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKND--ES 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N ALS+SIKAVCD TLYP+TC  AFG L+NSS LDPGQI K SVQLALGELSR ADY
Sbjct: 61  DNHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           + +HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATY
Sbjct: 121 LLEHAITNSTDNKTILALRSCHELLDLAMDHLNDSLSSSEITVLKAVDDLKTWLSSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYE+  S
Sbjct: 181 QQTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG Y
Sbjct: 241 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNY
Sbjct: 421 LQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID K
Sbjct: 481 STTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIHGKDWISKAQVPFNATL 566

[Close]

BLAST of Moc03g25220 vs. NCBI nr
Match: XP_022927225.1 (putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata])

HSP 1 Score: 941.0 bits (2431), Expect = 4.7e-270
Identity = 479/568 (84.33%), Postives = 514/568 (90.49%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D   +
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKND--ES 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N ALS+SIKAVCD TLYP+TC  AFG L+NSS LDPGQI K SVQLALGELSR ADY
Sbjct: 61  DNHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           + +HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATY
Sbjct: 121 LLEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYE+  S
Sbjct: 181 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG Y
Sbjct: 241 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNY
Sbjct: 421 LQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID K
Sbjct: 481 STTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIHGKDWISKAQVPFNATL 566

[Close]

BLAST of Moc03g25220 vs. ExPASy Swiss-Prot
Match: Q9SG77 (Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3702 GN=PME24 PE=3 SV=1)

HSP 1 Score: 590.5 bits (1521), Expect = 2.0e-167
Identity = 312/570 (54.74%), Postives = 407/570 (71.40%), Query Frame = 0


Query: 6   PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT--RNGSKDDGNGNGNG 65
           PYGKVDE + +RL+A R+TRK IA+I +S V+L  +V+  VFGT     S +    N NG
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  NSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFK 125
           +S +S S+KAVCDVTL+ E C    G   N+S L+P ++ + +V++ + E+S+A      
Sbjct: 64  DS-ISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI----- 123

Query: 126 HAITNST-DNRTKLALESCHELLDLALDHLNISLSST---EITLLKAVDDLKTWLTSAAT 185
           +A ++S  D +  + + +C ELLDL +D+LN +L+S+   ++T+ + VDDL+TWL+SA T
Sbjct: 124 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGT 183

Query: 186 YQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-RRLMSYENH 245
           YQ+TC+E L    P +R    ++LKNSTELTSN LAI+++  KI DS  L RRL++  + 
Sbjct: 184 YQRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADV 243

Query: 246 QSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKG 305
           +      R   R L   DLRK ADIVVAKDGSGKY+TI  AL+ VP+KS+K T+IYVKKG
Sbjct: 244 EVDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKG 303

Query: 306 TYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQ 365
            Y ENV+V K  WNV+++GDG + + V+ +LN IDGTPTF +ATFA  GKGF+A DMGF 
Sbjct: 304 VYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFI 363

Query: 366 NIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSA 425
           N AGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYRECT+ GTVDFIFGNSA
Sbjct: 364 NTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSA 423

Query: 426 VVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWK 485
            VLQ+C ILP+ P+ GQ+NTITAQGR DPN NTGISI   +I+P  +L    T+LGRPWK
Sbjct: 424 SVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWK 483

Query: 486 NYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNID 545
           N+STTV M +YL   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG+R + 
Sbjct: 484 NFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLS 543

Query: 546 NKTAKKFTVSSFIDGKDWISKAQVPFKSGL 569
            K A +FTV  FIDG  W+   +VPF+SGL
Sbjct: 544 TKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Moc03g25220 vs. ExPASy Swiss-Prot
Match: Q9FF78 (Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3702 GN=PME46 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.6e-164
Identity = 301/566 (53.18%), Postives = 398/566 (70.32%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNGNSA 66
           YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV  V GT            N    
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 126
           +S S+KA+CDVTL+ E C    G   N+SR  P ++ K +V++ + ELS+  D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 127 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIE 186
               DN T  A+ +C EL+ LA+D LN +++S+    LK  DDL+TWL+S  TYQ+TC++
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLEEVD-PTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQSSADWA 246
            L E + P+L     N+LKNSTE+TSN LAI+++  KI D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 -RPIL---RKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 306
             P++   R L   DL+K+A IVVAKDGSGKY+TI  AL  V +K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 366
           ENV V K KWNVVM+GDG + T V+A LNFIDGTPTF +ATFA  GKGF+A DMGF N A
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 486
           Q C ILP+ P+ GQ+NTITAQGR DPNQNTGISI + +I P +NL   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVR-NIDNK 546
           TTV M++++   I P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G++ ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKS 567
            A KFTV  FIDG +W+   +VPF S
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Moc03g25220 vs. ExPASy Swiss-Prot
Match: Q43111 (Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 4.1e-136
Identity = 270/582 (46.39%), Postives = 374/582 (64.26%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT---RNGSKDDGNGNGNG 66
           YGKV+E +Q   +  ++TRKR+ +I +SS+VL+AV++A V G       S+   + +   
Sbjct: 10  YGKVNELEQQAYE--KKTRKRLIIIAVSSIVLIAVIIAAVAGVVIHNRNSESSPSSDSVP 69

Query: 67  NSALS--SSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYV 126
            + LS  +S+KAVCD T YP +C  +   L  S+  DP  + KLS+++A+ ELS    + 
Sbjct: 70  QTELSPAASLKAVCDTTRYPSSCFSSISSLPESNTTDPELLFKLSLRVAIDELS---SFP 129

Query: 127 FKHAITNSTDNRTKLALESCHELLDLALDHLNISLSST-----EITLLKAVDDLKTWLTS 186
            K       D R + A++ C  +   ALD LN S+S+       I    +V +++TWL++
Sbjct: 130 SKLRANAEQDARLQKAIDVCSSVFGDALDRLNDSISALGTVAGRIASSASVSNVETWLSA 189

Query: 187 AATYQQTCIEGLEEVDPTLR----NSIANYLKNSTELTSNGLAIVS----FFSKITDSLN 246
           A T Q TC++ + E++ T        I   ++NSTE  SN LAIV+      S+    ++
Sbjct: 190 ALTDQDTCLDAVGELNSTAARGALQEIETAMRNSTEFASNSLAIVTKILGLLSRFETPIH 249

Query: 247 LRRLMSYENHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSK 306
            RRL+ +     +A+      R L +++     D VVAKDGSG++KTI  ALK V  KS+
Sbjct: 250 HRRLLGFPEWLGAAE-----RRLLEEKNNDSTPDAVVAKDGSGQFKTIGEALKLVKKKSE 309

Query: 307 KSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGK 366
           +   +YVK+G Y EN+++ KN WNV++ GDG + T V    NF+DGTPTF +ATFA KGK
Sbjct: 310 ERFSVYVKEGRYVENIDLDKNTWNVMIYGDGKDKTFVVGSRNFMDGTPTFETATFAVKGK 369

Query: 367 GFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEG 426
           GFIA D+GF N AG SKHQAVAL S SD+S+F+RC  D +QDTLYAHSNRQFYR+C + G
Sbjct: 370 GFIAKDIGFVNNAGASKHQAVALRSGSDRSVFFRCSFDGFQDTLYAHSNRQFYRDCDITG 429

Query: 427 TVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKA 486
           T+DFIFGN+AVV Q+C I+P+ PL  Q NTITAQG+ DPNQNTGI IQ S+ITP+ N   
Sbjct: 430 TIDFIFGNAAVVFQSCKIMPRQPLPNQFNTITAQGKKDPNQNTGIIIQKSTITPFGNNLT 489

Query: 487 TETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSSTAK 546
             TYLGRPWK++STTV MQ+ +G+L+ P GW+ WV     P TIFYAE+QN GPG+  ++
Sbjct: 490 APTYLGRPWKDFSTTVIMQSDIGALLNPVGWMSWVPNVEPPTTIFYAEYQNSGPGADVSQ 549

Query: 547 RVKWKGVR-NIDNKTAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           RVKW G +  I ++ A++FTV SFI G +W+  A V F S L
Sbjct: 550 RVKWAGYKPTITDRNAEEFTVQSFIQGPEWLPNAAVQFDSTL 581

[Close]

BLAST of Moc03g25220 vs. ExPASy Swiss-Prot
Match: Q43867 (Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1)

HSP 1 Score: 451.8 bits (1161), Expect = 1.1e-125
Identity = 254/573 (44.33%), Postives = 364/573 (63.53%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT-RNGSKDDGNGNGNGNS 66
           YGKVDE   L L   ++TRKR+ ++ +S VVL+AV++A V  T  + +K++   +     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 126
             S+S+KA+C VT +PE+C  +   L +S+  DP  + KLS+++ + EL   +D   K +
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLS 129

Query: 127 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLK------AVDDLKTWLTSAAT 186
              + D R K AL  C +L++ ALD LN ++S+ +    K       ++DLKTWL++  T
Sbjct: 130 -KETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIEGLEEV--------DPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-- 246
             +TC + L+E+        + T+  ++ + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYENHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDK 306
              RRLMS+ +HQ S D+ +   R+L Q    K  D+ VA DG+G   T++ A+  VP K
Sbjct: 250 HRRRRLMSH-HHQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAK 366
           S K  VIYVK GTY ENV + K+KWNV++ GDG   T ++   NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTV 426
           GKGFI  D+G  N AG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 EGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENL 486
            GT+DFIFG++AVV Q C I+P+ PL  Q NTITAQG+ DPNQ++G+SIQ  +I+   N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
            A  TYLGRPWK +STTV M+  +G++++P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 IA-PTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 AKRVKWKGVRNI-DNKTAKKFTVSSFIDGKDWI 558
            +RVKW G + +  +  A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

[Close]

BLAST of Moc03g25220 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 399.4 bits (1025), Expect = 6.6e-110
Identity = 234/566 (41.34%), Postives = 343/566 (60.60%), Query Frame = 0


Query: 19  DASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNGNSALSSSIKAVCDV- 78
           D S+R R+ I VI +SSV+L+++VVAV  G    S +  +G+  G + +++S+KAV DV 
Sbjct: 7   DESKRKRRYI-VITISSVLLISMVVAVTVGV---SLNKHDGDSKGKAEVNASVKAVKDVC 66

Query: 79  --TLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTK 138
             T Y +TC       +  +  DP ++ K +  + + +++ AA    +  +    D+RT+
Sbjct: 67  APTDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAK-KSQTIMELQKDSRTR 126

Query: 139 LALESCHELLDLALDHLNIS---LSSTEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEV 198
           +AL+ C EL+D ALD L+ S   L   E  LL +A+ +L+ WL++A ++++TC+EG +  
Sbjct: 127 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 186

Query: 199 DPTLRNSIANYLKNSTELTSNGLAIVSFFSKIT-----DSLNLRRLMSYENHQSSADWAR 258
                 ++   LK + ELT NGLAI+S  S          LN RRL++ E   S  D   
Sbjct: 187 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVDQRG 246

Query: 259 PILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEV 318
             L + A      + DIVVA+DGSG+YKTI+ AL+ VP K   + V+++K G YKE V+V
Sbjct: 247 RKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQV 306

Query: 319 GKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKH 378
            K   ++V IGDG + T ++   N+ DG  T+ +AT A  G  FIA ++GF+N AG  KH
Sbjct: 307 NKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKH 366

Query: 379 QAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTI 438
           QAVA+   SD+SIF+ C+ D YQDTLY HS+RQF+R+CT+ GT+DF+FG++A V QNCT+
Sbjct: 367 QAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTL 426

Query: 439 LPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSIT---PYENLKAT-ETYLGRPWKNYST 498
           L + PL  Q   ITA GR DP ++TG   Q  +I     Y  +K T + YLGRPWK YS 
Sbjct: 427 LVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSR 486

Query: 499 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 558
           T+ M  ++   +QP GW PW+G     T+FY+E QN GPGS+ A RV W G++ +  +  
Sbjct: 487 TIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDI 546

Query: 559 KKFTVSSFIDGKDWISKAQVPFKSGL 569
            KFT + +I G DWI    VP+ +GL
Sbjct: 547 LKFTPAQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of Moc03g25220 vs. ExPASy TrEMBL
Match: A0A6J1CDY3 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1)

HSP 1 Score: 1118.2 bits (2891), Expect = 0.0e+00
Identity = 568/568 (100.00%), Postives = 568/568 (100.00%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN
Sbjct: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
           GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY
Sbjct: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY
Sbjct: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS
Sbjct: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY
Sbjct: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI
Sbjct: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY
Sbjct: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK
Sbjct: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPFKSGL
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 568

[Close]

BLAST of Moc03g25220 vs. ExPASy TrEMBL
Match: A0A6J1KL23 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.0e-271
Identity = 480/568 (84.51%), Postives = 515/568 (90.67%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D   +
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKND--ES 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N ALS+SIKAVCD TLYP+TCN AFGPL+NSS LDPGQI K SVQLAL ELSR ADY
Sbjct: 61  DNHNEALSTSIKAVCDETLYPDTCNNAFGPLANSSHLDPGQIAKFSVQLALSELSRVADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           + +HAITNSTDN+T LAL  CHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATY
Sbjct: 121 LLEHAITNSTDNKTILALRGCHELLDLAMDHLNDSLSSSEITMLKAVDDLKTWLSSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYE+  S
Sbjct: 181 QQTCIDGLEEVDLALMNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ ALKAV DKSKK TVIYVKKG Y
Sbjct: 241 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALKAVRDKSKKRTVIYVKKGVY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEV KNKWNVVMIGDGM +TTVT KLNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVVKNKWNVVMIGDGMTSTTVTGKLNFIDGTPTFSTATFAAKGKGFIAIDMGFENT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK T+TYLGRPWKNY
Sbjct: 421 LQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTQTYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID K
Sbjct: 481 STTVFMQNNFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFNATL 566

[Close]

BLAST of Moc03g25220 vs. ExPASy TrEMBL
Match: A0A6J1ENB0 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1)

HSP 1 Score: 941.0 bits (2431), Expect = 2.3e-270
Identity = 479/568 (84.33%), Postives = 514/568 (90.49%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MSLLRPYGKVDE+DQ+RLDASRRTRKR+ VIVLSSVVLV VVVAVVFGTRN SK+D   +
Sbjct: 1   MSLLRPYGKVDESDQMRLDASRRTRKRVIVIVLSSVVLVGVVVAVVFGTRNSSKND--ES 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N ALS+SIKAVCD TLYP+TC  AFG L+NSS LDPGQI K SVQLALGELSR ADY
Sbjct: 61  DNHNEALSTSIKAVCDETLYPDTCKNAFGSLANSSHLDPGQIAKFSVQLALGELSRVADY 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           + +HAITNSTDN+T LAL SCHELLDLA+DHLN SLSS+EIT+LKAVDDLKTWL+SAATY
Sbjct: 121 LLEHAITNSTDNKTILALRSCHELLDLAIDHLNDSLSSSEITVLKAVDDLKTWLSSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GLEEVD  L N IANYLKNSTE+TSNGLAIVSFFSK+TDS +LRRLMSYE+  S
Sbjct: 181 QQTCIDGLEEVDLALTNVIANYLKNSTEMTSNGLAIVSFFSKLTDSFSLRRLMSYEDRHS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ+DLRKQADIVVAKD SGKYKTI+ AL AVPDKSKK TVIYVKKG Y
Sbjct: 241 NGDWARPILRKLAQKDLRKQADIVVAKDKSGKYKTITEALNAVPDKSKKRTVIYVKKGVY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KENVEV KNKWNVVMIGDGM +TTVT +LNFIDGTPTFS+ATFAAKGKGFIAIDMGF+N 
Sbjct: 301 KENVEVVKNKWNVVMIGDGMTSTTVTGRLNFIDGTPTFSTATFAAKGKGFIAIDMGFENT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYR+C V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRDCKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNC I PKLPLLGQKNTITAQGRFDPNQ+TGISIQ SSI P+ENLK TETYLGRPWKNY
Sbjct: 421 LQNCKIAPKLPLLGQKNTITAQGRFDPNQHTGISIQHSSIQPFENLKTTETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQN  GSLI PTGWLPWVGTSAPDTIFYAEFQN GPGSSTA RVKWKG++NID K
Sbjct: 481 STTVFMQNDFGSLIHPTGWLPWVGTSAPDTIFYAEFQNKGPGSSTANRVKWKGLKNIDTK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFI GKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIHGKDWISKAQVPFNATL 566

[Close]

BLAST of Moc03g25220 vs. ExPASy TrEMBL
Match: A0A5A7VDG1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G00770 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.8e-270
Identity = 476/568 (83.80%), Postives = 519/568 (91.37%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLVAVVVAVVFGTRN S ++ + N
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N +LS+SIKAVCDVTLYP+TC  AFGP +NSS LDPGQI KL+VQLALGELS+ AD+
Sbjct: 61  -NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADH 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           +F HAITN+TD++T LAL++CHELLDLALDHLNI+LSS++ITLLKAVDDLKTWLTSAATY
Sbjct: 121 IFDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GL EVDP L +  AN+LKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYE+ QS
Sbjct: 181 QQTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ D+RKQADIVVAKD SGKYKTI+ ALKAVPDKSKK TVIYVKKGTY
Sbjct: 241 NGDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KE VEV KNKWNVVM+GDGMNAT V+  LN IDGTPTFS+A+FAAKGKGFIAIDMGF N 
Sbjct: 301 KEKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYREC V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQ S I P++NLK TETYLGRPWKNY
Sbjct: 421 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKWKGV+NID K
Sbjct: 481 STTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Moc03g25220 vs. ExPASy TrEMBL
Match: A0A1S3BDA4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1)

HSP 1 Score: 940.3 bits (2429), Expect = 3.8e-270
Identity = 476/568 (83.80%), Postives = 519/568 (91.37%), Query Frame = 0


Query: 1   MSLLRPYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGN 60
           MS LRPYGKVDETDQLRLDASRRTR+RI VIVLSSVVLVAVVVAVVFGTRN S ++ + N
Sbjct: 1   MSFLRPYGKVDETDQLRLDASRRTRRRIVVIVLSSVVLVAVVVAVVFGTRNASNNNDDSN 60

Query: 61  GNGNSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADY 120
            N N +LS+SIKAVCDVTLYP+TC  AFGP +NSS LDPGQI KL+VQLALGELS+ AD+
Sbjct: 61  -NQNGSLSASIKAVCDVTLYPDTCQKAFGPFANSSHLDPGQISKLAVQLALGELSKVADH 120

Query: 121 VFKHAITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATY 180
           +F HAITN+TD++T LAL++CHELLDLALDHLNI+LSS++ITLLKAVDDLKTWLTSAATY
Sbjct: 121 IFDHAITNTTDDKTILALKNCHELLDLALDHLNITLSSSDITLLKAVDDLKTWLTSAATY 180

Query: 181 QQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQS 240
           QQTCI+GL EVDP L +  AN+LKNSTELTSNGLAIVSFFSK+TDSL+LRRLMSYE+ QS
Sbjct: 181 QQTCIDGLAEVDPALADLFANFLKNSTELTSNGLAIVSFFSKLTDSLSLRRLMSYEDRQS 240

Query: 241 SADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTY 300
           + DWARPILRKLAQ D+RKQADIVVAKD SGKYKTI+ ALKAVPDKSKK TVIYVKKGTY
Sbjct: 241 NGDWARPILRKLAQSDIRKQADIVVAKDSSGKYKTITEALKAVPDKSKKRTVIYVKKGTY 300

Query: 301 KENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNI 360
           KE VEV KNKWNVVM+GDGMNAT V+  LN IDGTPTFS+A+FAAKGKGFIAIDMGF N 
Sbjct: 301 KEKVEVVKNKWNVVMVGDGMNATIVSGSLNVIDGTPTFSTASFAAKGKGFIAIDMGFINT 360

Query: 361 AGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVV 420
           AGPSKHQAVALMSTSDQSIFYRC+M+AYQDTLYAHSNRQFYREC V GTVDFIFGNSAVV
Sbjct: 361 AGPSKHQAVALMSTSDQSIFYRCQMNAYQDTLYAHSNRQFYRECKVYGTVDFIFGNSAVV 420

Query: 421 LQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNY 480
           LQNCTI P+LPLLGQKNTITAQGRFDPNQNTGISIQ S I P++NLK TETYLGRPWKNY
Sbjct: 421 LQNCTIAPRLPLLGQKNTITAQGRFDPNQNTGISIQGSLIQPFDNLKTTETYLGRPWKNY 480

Query: 481 STTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNK 540
           STTVFMQ+ LGSLI P+GWLPWVGTSAPDTIFYAEFQNYG GSST+KRVKWKGV+NID K
Sbjct: 481 STTVFMQSNLGSLIHPSGWLPWVGTSAPDTIFYAEFQNYGRGSSTSKRVKWKGVKNIDIK 540

Query: 541 TAKKFTVSSFIDGKDWISKAQVPFKSGL 569
           TAKKFTVSSFIDGKDWISKAQVPF + L
Sbjct: 541 TAKKFTVSSFIDGKDWISKAQVPFTATL 567

[Close]

BLAST of Moc03g25220 vs. TAIR 10
Match: AT3G10710.1 (root hair specific 12 )

HSP 1 Score: 590.5 bits (1521), Expect = 1.4e-168
Identity = 312/570 (54.74%), Postives = 407/570 (71.40%), Query Frame = 0


Query: 6   PYGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT--RNGSKDDGNGNGNG 65
           PYGKVDE + +RL+A R+TRK IA+I +S V+L  +V+  VFGT     S +    N NG
Sbjct: 4   PYGKVDEREHVRLEARRKTRKNIAIIAVSLVILAGIVIGAVFGTMAHKKSPETVETNNNG 63

Query: 66  NSALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFK 125
           +S +S S+KAVCDVTL+ E C    G   N+S L+P ++ + +V++ + E+S+A      
Sbjct: 64  DS-ISVSVKAVCDVTLHKEKCFETLGSAPNASSLNPEELFRYAVKITIAEVSKAI----- 123

Query: 126 HAITNST-DNRTKLALESCHELLDLALDHLNISLSST---EITLLKAVDDLKTWLTSAAT 185
           +A ++S  D +  + + +C ELLDL +D+LN +L+S+   ++T+ + VDDL+TWL+SA T
Sbjct: 124 NAFSSSLGDEKNNITMNACAELLDLTIDNLNNTLTSSSNGDVTVPELVDDLRTWLSSAGT 183

Query: 186 YQQTCIEGLEEVDPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-RRLMSYENH 245
           YQ+TC+E L    P +R    ++LKNSTELTSN LAI+++  KI DS  L RRL++  + 
Sbjct: 184 YQRTCVETLA---PDMRPFGESHLKNSTELTSNALAIITWLGKIADSFKLRRRLLTTADV 243

Query: 246 QSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKG 305
           +      R   R L   DLRK ADIVVAKDGSGKY+TI  AL+ VP+KS+K T+IYVKKG
Sbjct: 244 EVDFHAGR---RLLQSTDLRKVADIVVAKDGSGKYRTIKRALQDVPEKSEKRTIIYVKKG 303

Query: 306 TYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQ 365
            Y ENV+V K  WNV+++GDG + + V+ +LN IDGTPTF +ATFA  GKGF+A DMGF 
Sbjct: 304 VYFENVKVEKKMWNVIVVGDGESKSIVSGRLNVIDGTPTFKTATFAVFGKGFMARDMGFI 363

Query: 366 NIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSA 425
           N AGPSKHQAVALM ++D + FYRC M+AYQDTLY H+ RQFYRECT+ GTVDFIFGNSA
Sbjct: 364 NTAGPSKHQAVALMVSADLTAFYRCTMNAYQDTLYVHAQRQFYRECTIIGTVDFIFGNSA 423

Query: 426 VVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWK 485
            VLQ+C ILP+ P+ GQ+NTITAQGR DPN NTGISI   +I+P  +L    T+LGRPWK
Sbjct: 424 SVLQSCRILPRRPMKGQQNTITAQGRTDPNMNTGISIHRCNISPLGDLTDVMTFLGRPWK 483

Query: 486 NYSTTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNID 545
           N+STTV M +YL   I   GWLPW G SAPDTIFY E++N GPG+ST  RVKWKG+R + 
Sbjct: 484 NFSTTVIMDSYLHGFIDRKGWLPWTGDSAPDTIFYGEYKNTGPGASTKNRVKWKGLRFLS 543

Query: 546 NKTAKKFTVSSFIDGKDWISKAQVPFKSGL 569
            K A +FTV  FIDG  W+   +VPF+SGL
Sbjct: 544 TKEANRFTVKPFIDGGRWLPATKVPFRSGL 561

[Close]

BLAST of Moc03g25220 vs. TAIR 10
Match: AT5G04960.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 580.9 bits (1496), Expect = 1.1e-165
Identity = 301/566 (53.18%), Postives = 398/566 (70.32%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNGNSA 66
           YG++DE +Q +L+ASR+T+KRIA+I +SS+VLV +VV  V GT            N    
Sbjct: 4   YGRLDEHEQAKLEASRKTKKRIAIIAISSIVLVCIVVGAVVGTTARDNSKKPPTENNGEP 63

Query: 67  LSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHAI 126
           +S S+KA+CDVTL+ E C    G   N+SR  P ++ K +V++ + ELS+  D     + 
Sbjct: 64  ISVSVKALCDVTLHKEKCFETLGSAPNASRSSPEELFKYAVKVTITELSKVLD---GFSN 123

Query: 127 TNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLKAVDDLKTWLTSAATYQQTCIE 186
               DN T  A+ +C EL+ LA+D LN +++S+    LK  DDL+TWL+S  TYQ+TC++
Sbjct: 124 GEHMDNATSAAMGACVELIGLAVDQLNETMTSS----LKNFDDLRTWLSSVGTYQETCMD 183

Query: 187 GLEEVD-PTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNLRRLMSYENHQSSADWA 246
            L E + P+L     N+LKNSTE+TSN LAI+++  KI D++  RR    E   +    A
Sbjct: 184 ALVEANKPSLTTFGENHLKNSTEMTSNALAIITWLGKIADTVKFRRRRLLETGNAKVVVA 243

Query: 247 -RPIL---RKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYK 306
             P++   R L   DL+K+A IVVAKDGSGKY+TI  AL  V +K++K T+IYVKKG Y 
Sbjct: 244 DLPMMEGRRLLESGDLKKKATIVVAKDGSGKYRTIGEALAEVEEKNEKPTIIYVKKGVYL 303

Query: 307 ENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIA 366
           ENV V K KWNVVM+GDG + T V+A LNFIDGTPTF +ATFA  GKGF+A DMGF N A
Sbjct: 304 ENVRVEKTKWNVVMVGDGQSKTIVSAGLNFIDGTPTFETATFAVFGKGFMARDMGFINTA 363

Query: 367 GPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVL 426
           GP+KHQAVALM ++D S+FY+C MDA+QDT+YAH+ RQFYR+C + GTVDFIFGN+AVV 
Sbjct: 364 GPAKHQAVALMVSADLSVFYKCTMDAFQDTMYAHAQRQFYRDCVILGTVDFIFGNAAVVF 423

Query: 427 QNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATETYLGRPWKNYS 486
           Q C ILP+ P+ GQ+NTITAQGR DPNQNTGISI + +I P +NL   +T+LGRPWK++S
Sbjct: 424 QKCEILPRRPMKGQQNTITAQGRKDPNQNTGISIHNCTIKPLDNLTDIQTFLGRPWKDFS 483

Query: 487 TTVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVR-NIDNK 546
           TTV M++++   I P GWLPW G +APDTIFYAE+ N GPG+ST  RVKW+G++ ++  K
Sbjct: 484 TTVIMKSFMDKFINPKGWLPWTGDTAPDTIFYAEYLNSGPGASTKNRVKWQGLKTSLTKK 543

Query: 547 TAKKFTVSSFIDGKDWISKAQVPFKS 567
            A KFTV  FIDG +W+   +VPF S
Sbjct: 544 EANKFTVKPFIDGNNWLPATKVPFNS 562

[Close]

BLAST of Moc03g25220 vs. TAIR 10
Match: AT1G53840.1 (pectin methylesterase 1 )

HSP 1 Score: 451.8 bits (1161), Expect = 8.0e-127
Identity = 254/573 (44.33%), Postives = 364/573 (63.53%), Query Frame = 0


Query: 7   YGKVDETDQLRLDASRRTRKRIAVIVLSSVVLVAVVVAVVFGT-RNGSKDDGNGNGNGNS 66
           YGKVDE   L L   ++TRKR+ ++ +S VVL+AV++A V  T  + +K++   +     
Sbjct: 10  YGKVDEAQDLAL--KKKTRKRLLLLSISVVVLIAVIIAAVVATVVHKNKNESTPSPPPEL 69

Query: 67  ALSSSIKAVCDVTLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 126
             S+S+KA+C VT +PE+C  +   L +S+  DP  + KLS+++ + EL   +D   K +
Sbjct: 70  TPSTSLKAICSVTRFPESCISSISKLPSSNTTDPETLFKLSLKVIIDELDSISDLPEKLS 129

Query: 127 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITLLK------AVDDLKTWLTSAAT 186
              + D R K AL  C +L++ ALD LN ++S+ +    K       ++DLKTWL++  T
Sbjct: 130 -KETEDERIKSALRVCGDLIEDALDRLNDTVSAIDDEEKKKTLSSSKIEDLKTWLSATVT 189

Query: 187 YQQTCIEGLEEV--------DPTLRNSIANYLKNSTELTSNGLAIVSFFSKITDSLNL-- 246
             +TC + L+E+        + T+  ++ + +  STE TSN LAIVS        L +  
Sbjct: 190 DHETCFDSLDELKQNKTEYANSTITQNLKSAMSRSTEFTSNSLAIVSKILSALSDLGIPI 249

Query: 247 ---RRLMSYENHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDK 306
              RRLMS+ +HQ S D+ +   R+L Q    K  D+ VA DG+G   T++ A+  VP K
Sbjct: 250 HRRRRLMSH-HHQQSVDFEKWARRRLLQTAGLK-PDVTVAGDGTGDVLTVNEAVAKVPKK 309

Query: 307 SKKSTVIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAK 366
           S K  VIYVK GTY ENV + K+KWNV++ GDG   T ++   NF+DGTPT+ +ATFA +
Sbjct: 310 SLKMFVIYVKSGTYVENVVMDKSKWNVMIYGDGKGKTIISGSKNFVDGTPTYETATFAIQ 369

Query: 367 GKGFIAIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTV 426
           GKGFI  D+G  N AG +KHQAVA  S SD S++Y+C  D +QDTLY HSNRQFYR+C V
Sbjct: 370 GKGFIMKDIGIINTAGAAKHQAVAFRSGSDFSVYYQCSFDGFQDTLYPHSNRQFYRDCDV 429

Query: 427 EGTVDFIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENL 486
            GT+DFIFG++AVV Q C I+P+ PL  Q NTITAQG+ DPNQ++G+SIQ  +I+   N+
Sbjct: 430 TGTIDFIFGSAAVVFQGCKIMPRQPLSNQFNTITAQGKKDPNQSSGMSIQRCTISANGNV 489

Query: 487 KATETYLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWV-GTSAPDTIFYAEFQNYGPGSST 546
            A  TYLGRPWK +STTV M+  +G++++P+GW+ WV G   P +I Y E++N GPGS  
Sbjct: 490 IA-PTYLGRPWKEFSTTVIMETVIGAVVRPSGWMSWVSGVDPPASIVYGEYKNTGPGSDV 549

Query: 547 AKRVKWKGVRNI-DNKTAKKFTVSSFIDGKDWI 558
            +RVKW G + +  +  A KFTV++ + G DWI
Sbjct: 550 TQRVKWAGYKPVMSDAEAAKFTVATLLHGADWI 576

[Close]

BLAST of Moc03g25220 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 399.4 bits (1025), Expect = 4.7e-111
Identity = 234/566 (41.34%), Postives = 343/566 (60.60%), Query Frame = 0


Query: 19  DASRRTRKRIAVIVLSSVVLVAVVVAVVFGTRNGSKDDGNGNGNGNSALSSSIKAVCDV- 78
           D S+R R+ I VI +SSV+L+++VVAV  G    S +  +G+  G + +++S+KAV DV 
Sbjct: 7   DESKRKRRYI-VITISSVLLISMVVAVTVGV---SLNKHDGDSKGKAEVNASVKAVKDVC 66

Query: 79  --TLYPETCNYAFGPLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHAITNSTDNRTK 138
             T Y +TC       +  +  DP ++ K +  + + +++ AA    +  +    D+RT+
Sbjct: 67  APTDYRKTCEDTL-IKNGKNTTDPMELVKTAFNVTMKQITDAAK-KSQTIMELQKDSRTR 126

Query: 139 LALESCHELLDLALDHLNIS---LSSTEITLL-KAVDDLKTWLTSAATYQQTCIEGLEEV 198
           +AL+ C EL+D ALD L+ S   L   E  LL +A+ +L+ WL++A ++++TC+EG +  
Sbjct: 127 MALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQGT 186

Query: 199 DPTLRNSIANYLKNSTELTSNGLAIVSFFSKIT-----DSLNLRRLMSYENHQSSADWAR 258
                 ++   LK + ELT NGLAI+S  S          LN RRL++ E   S  D   
Sbjct: 187 QGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA-EGFPSWVDQRG 246

Query: 259 PILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKSTVIYVKKGTYKENVEV 318
             L + A      + DIVVA+DGSG+YKTI+ AL+ VP K   + V+++K G YKE V+V
Sbjct: 247 RKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPKKRNTTFVVHIKAGLYKEYVQV 306

Query: 319 GKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFIAIDMGFQNIAGPSKH 378
            K   ++V IGDG + T ++   N+ DG  T+ +AT A  G  FIA ++GF+N AG  KH
Sbjct: 307 NKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKH 366

Query: 379 QAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVDFIFGNSAVVLQNCTI 438
           QAVA+   SD+SIF+ C+ D YQDTLY HS+RQF+R+CT+ GT+DF+FG++A V QNCT+
Sbjct: 367 QAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTL 426

Query: 439 LPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSIT---PYENLKAT-ETYLGRPWKNYST 498
           L + PL  Q   ITA GR DP ++TG   Q  +I     Y  +K T + YLGRPWK YS 
Sbjct: 427 LVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLGRPWKEYSR 486

Query: 499 TVFMQNYLGSLIQPTGWLPWVGTSAPDTIFYAEFQNYGPGSSTAKRVKWKGVRNIDNKTA 558
           T+ M  ++   +QP GW PW+G     T+FY+E QN GPGS+ A RV W G++ +  +  
Sbjct: 487 TIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDI 546

Query: 559 KKFTVSSFIDGKDWISKAQVPFKSGL 569
            KFT + +I G DWI    VP+ +GL
Sbjct: 547 LKFTPAQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of Moc03g25220 vs. TAIR 10
Match: AT3G14300.1 (pectinesterase family protein )

HSP 1 Score: 396.0 bits (1016), Expect = 5.2e-110
Identity = 234/513 (45.61%), Postives = 310/513 (60.43%), Query Frame = 0


Query: 68  SSSIKAVCDVTLYPETCNYAFG--PLSNSSRLDPGQICKLSVQLALGELSRAADYVFKHA 127
           SS ++ VC+VT YP +C  +    PLS ++  DP  + +LS+Q+   EL+       K A
Sbjct: 456 SSVLRTVCNVTNYPASCISSISKLPLSKTT-TDPKVLFRLSLQVTFDELNSIVGLPKKLA 515

Query: 128 ITNSTDNRTKLALESCHELLDLALDHLNISLSSTEITL--------LKAVDDLKTWLTSA 187
              + D   K AL  C ++ DLA+D +N ++SS +  +           + DL TWL+SA
Sbjct: 516 -EETNDEGLKSALSVCADVFDLAVDSVNDTISSLDEVISGGKKNLNSSTIGDLITWLSSA 575

Query: 188 ATYQQTCIEGLEE--VDPTLRNSIANYLKNSTELTSNGLAIVSFF----SKITDSLNLRR 247
            T   TC + L+E   +  +   + + + NSTE TSN LAIV+      SK    +  RR
Sbjct: 576 VTDIGTCGDTLDEDNYNSPIPQKLKSAMVNSTEFTSNSLAIVAQVLKKPSKSRIPVQGRR 635

Query: 248 LMSYENHQSSADWARPILRKLAQRDLRKQADIVVAKDGSGKYKTISAALKAVPDKSKKST 307
           L+   N  S  +W RP +R+L Q        + VA DGSG  +T++ A+  VP K K   
Sbjct: 636 LL---NSNSFPNWVRPGVRRLLQAK-NLTPHVTVAADGSGDVRTVNEAVWRVPKKGKTMF 695

Query: 308 VIYVKKGTYKENVEVGKNKWNVVMIGDGMNATTVTAKLNFIDGTPTFSSATFAAKGKGFI 367
           VIYVK GTY ENV + K+KWNV + GDG + T ++   N +DG  TF+++TFA +GKGF+
Sbjct: 696 VIYVKAGTYVENVLMKKDKWNVFIYGDGRDKTIISGSTNMVDGVRTFNTSTFATEGKGFM 755

Query: 368 AIDMGFQNIAGPSKHQAVALMSTSDQSIFYRCKMDAYQDTLYAHSNRQFYRECTVEGTVD 427
             DMG  N AGP KHQAVA  S SD+S++YRC  D YQDTLY HSNRQ+YR C V GTVD
Sbjct: 756 MKDMGIINTAGPEKHQAVAFRSDSDRSVYYRCSFDGYQDTLYTHSNRQYYRNCDVTGTVD 815

Query: 428 FIFGNSAVVLQNCTILPKLPLLGQKNTITAQGRFDPNQNTGISIQDSSITPYENLKATET 487
           FIFG   VV Q C+I P+ PL  Q NTITA+G  + NQNTGISI   +I+P  N+ AT T
Sbjct: 816 FIFGAGTVVFQGCSIRPRQPLPNQFNTITAEGTQEANQNTGISIHQCTISPNGNVTAT-T 875

Query: 488 YLGRPWKNYSTTVFMQNYLGSLIQPTGWLPWVGT--SAPDTIFYAEFQNYGPGSSTAKRV 547
           YLGRPWK +S TV MQ+ +GS + P GW+ W  T    P TIFY E++N GPGS  +KRV
Sbjct: 876 YLGRPWKLFSKTVIMQSVIGSFVNPAGWIAWNSTYDPPPRTIFYREYKNSGPGSDLSKRV 935

Query: 548 KWKGVRNI-DNKTAKKFTVSSFIDGKD-WISKA 561
           KW G + I  +  A +FTV  F+ G D WI KA
Sbjct: 936 KWAGYKPISSDDEAARFTVKYFLRGDDNWIPKA 961

The following BLAST results are available for this feature:

BLAST of Moc03g25220 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140040.1	0.0e+00	100.00	putative pectinesterase/pectinesterase inhibitor 24 [Momordica charantia]	[more]
XP_038895610.1	2.5e-279	86.80	putative pectinesterase/pectinesterase inhibitor 24 [Benincasa hispida]	[more]
XP_023000829.1	4.2e-271	84.51	probable pectinesterase/pectinesterase inhibitor 46 [Cucurbita maxima]	[more]
KAG6583848.1	4.7e-270	84.33	putative pectinesterase/pectinesterase inhibitor 24, partial [Cucurbita argyrosp...	[more]
XP_022927225.1	4.7e-270	84.33	putative pectinesterase/pectinesterase inhibitor 24 [Cucurbita moschata]	[more]

BLAST of Moc03g25220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SG77	2.0e-167	54.74	Putative pectinesterase/pectinesterase inhibitor 24 OS=Arabidopsis thaliana OX=3...	[more]
Q9FF78	1.6e-164	53.18	Probable pectinesterase/pectinesterase inhibitor 46 OS=Arabidopsis thaliana OX=3...	[more]
Q43111	4.1e-136	46.39	Pectinesterase 3 OS=Phaseolus vulgaris OX=3885 GN=MPE3 PE=2 SV=1	[more]
Q43867	1.1e-125	44.33	Pectinesterase 1 OS=Arabidopsis thaliana OX=3702 GN=PME1 PE=1 SV=1	[more]
Q8GX86	6.6e-110	41.34	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of Moc03g25220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CDY3	0.0e+00	100.00	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111010791 PE=3 SV=1	[more]
A0A6J1KL23	2.0e-271	84.51	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111495161 PE=3 SV=1	[more]
A0A6J1ENB0	2.3e-270	84.33	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434138 PE=3 SV=1	[more]
A0A5A7VDG1	3.8e-270	83.80	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1290G0077...	[more]
A0A1S3BDA4	3.8e-270	83.80	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103488419 PE=3 SV=1	[more]

BLAST of Moc03g25220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10710.1	1.4e-168	54.74	root hair specific 12	[more]
AT5G04960.1	1.1e-165	53.18	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G53840.1	8.0e-127	44.33	pectin methylesterase 1	[more]
AT3G05610.1	4.7e-111	41.34	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G14300.1	5.2e-110	45.61	pectinesterase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 66..216 e-value: 1.6E-33 score: 127.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 64..217 e-value: 2.7E-24 score: 83.4
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 70..216 e-value: 2.8E-25 score: 89.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 68..220 e-value: 1.5E-31 score: 111.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 69..221
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 262..553 e-value: 1.0E-138 score: 461.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 257..568 e-value: 1.8E-114 score: 384.5
None	No IPR available	PANTHER	PTHR31707:SF272	PECTINESTERASE/PECTINESTERASE INHIBITOR 24-RELATED	coord: 19..564
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 19..564
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 73..222 e-value: 5.94877E-40 score: 140.651
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 287..306
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 406..415
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 260..566

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g25220.1	Moc03g25220.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity