Moc03g25200 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g25200
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	3-hydroxyisobutyrate dehydrogenase
Location	chr3: 18101382 .. 18108501 (-)
RNA-Seq Expression	Moc03g25200
Synteny	Moc03g25200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGGTTTCTAGAGTAAGAGCTTCTTTTGCGCAGTACATGATCAAATCTAATTTCAAACATTTTCCGGGTGAGACTGCGACGCCTTCCGTGCGTAGATTCTTCTCTGCGGATGCTTTGGCTTCGTTCCAGGCAAGTTATTTTCATATCTGGAATCATCTGTTTGTCTGCCTTTGCTTGATTGTTGAGAAAAATGATGGGATGGGGTTCTTTTTTTTTTTTTATCTTCTACTTTTTTAAATGTATTTCTAGCCCGTAGAGGCAAATGATGATTGGTCTTTTTGGGAATTCAACGAACTGGAAAACTTTTAGTTTGAACTGGTGGAAATATTTCGAGGTTTAATTAATATGCTCTACTAATGTTTATTGTGAGGTCGAGAATGACAAATGATTTGTGGTTGTACTTTTGAAAGGAACACGAAAGTTCAAAGAGTAGTGCTAGTTACCTTTATGCTTATGAATCCAATAATGCCATAAAATCTACACAAATTTTATCATCTCTCACATTATTAAAATAAAATGTCACATCATGGAACCTATATGTCACATCTACAAATGATTTGTAAAGTTAGATTTTGTTACTCTCCGATTATGATAGAAATTCTTAGTAAGCTTTTTGAACTATGGCCAATAAAAGAACTTCTAAAAGTGTAGGGACAAAATGTAATCTAACCTTACGTTTAAATCTATAATAATTCATTGGGTTGATCAAGGTTGTTTTAGTTTTTCGTTATTGTTCTATAAAAAAATTATAACACAACGACTAAGAGAGTAGATGAAGTATGGTAATGCATTAAATTGGCTTTTAACGTCTTCCAAGATCCTTCAATTGTTGTCTCAAAAAAAAAAAAAGGATCCTTTAATTGTTTAGAATCACATCGCTTAAAACAGTTTTCAAGATTTTGGATTTAATGTTGCCTACACATTCTCCGTATTCCTAGTTTTTACAAATATAAAACTGTTTTTGGCTCTCAGAACTAAAAAAACCAACAAAATTCTATGTAAATTAACAATTTTCTGGAATCATCTCGTTTCGAGCAATGCCGGCTATTCTTGGCTCCTGAATATTGCTTTTTGTACATAAGATGGAGATTTTCTCTGTCTCTGACAGAGATAAGGATGATAGAAAGTTAATCTCTCACATACTTTTTGATTTTCATATGTGAAATCTCAGAGTGTGAAGTAATGTGACACAAAAATTTGTTATTCATTATGAGTTAAATTTTCAAGGATGCAATGTAACTTATTCAGTCTGACTTGGACCTTAGTTTAACAGTTTTTGTTACTCGATGACAAAGGGTTACATTTTATTATTAAGCTGTAGAAGTCTTATTGAAAACTTGAACCTTTCAACCTAATTGATGCTCTTTGACGATTGTGATTTGGTATTTGTGTACTTAACATTGTATCTTGAAGACCCAAGATCGTCTACTTTCTGACTCTGTAAAATGTGGCTTGCTGCTATTCAATTCGTAATTTCACACTCCTTAATTCAATGATTACGAAATGTGGATCTCATCATCTTTCACGTTCCCATTCTGGCTTGTTTTCAGACTAAGAAAAAGTGCCTTCAACAATGTTTTGCAGAGTGTTGGATTCATTGGACTGGGAAATATGGGATCCAGAATGGCAAATAATCTAATAAAGGCTGGATACAAAATGGTTGTACATGACATGTAATGCACTTGAATCTTCTGTTGCAGCCAAAGGCTATTGTCAAGGTTTTCATAAACTGTTCTTTTATTTTAGAAAAAAAAAATGTAATGGATTAATAATGACTTAACCCATTTGGAAACAATTATTTCAAATGATTACAAAGTTTCAAATAGAATCTCAATAATTTGATCTTTTTTTTTTTTTTTAACTGAAAAACTATGGAACTTTATTATATTTGGCTCAACTGGAGGCTACAAGGTAGCCAAAAGATGATCCAAGCCAAAATTTATGTTTGTATTGTATTGAAACATTTCATCTTTTTAATTTCTACGCAAAAGCTCTAAAAACATGTTCTCATGGTTGGACAGTGGTGAATCTAGTAGGAAATATACTTGAGTGAAGCTGAATCTGATTTATTTTTCTATGGAAAACATTCTTTTTGCTTCTCTAATTGAAAAAACGACAACAGTTTTACTAATGCTGACTGTGCATAATATTGTTTCCTATTAGAAACCGTGATACCATGAAGAAGTACTCAGATATGGGAGTTTCCACCAAGGAAACACCCTTTGAAGTTGCAGAAGCTTCTGACATTGTCATTACGATGCTACCCTCTTCTTCTCATGTGAGCCGTGACATGTTTTTTTCCCCTTTCCTCCTGAAGGGGTCTATTAGTGACCTGCATTCTTTATTTTGTAAGGCTATGACTTGCGTGTTTTATGATTTGACCTTGCATTATATTCTCCTTCATTCAAGTTAGAACTTCTATGTTCCATTATAGGTTTGGTTTGTTATAAGCATTTTTTAGGTATGTGCATGACAATATTCATGAGATTTTCGATATACATTTGCAATTACTATGTACGTGAAAGTGTTTTTTTAAAAAAATTGTAATGGATTAATAACGATAGAGTGTCATCCACTGATTCCAGCTTGTGAAATTCCAAATTTTACTCTGCATGTTTTTATGATGTAAAAAGCTTTTAGAATATTTTGAAGATTTGCATGGTTTTATTGTAATGATCTTATTTTCTGCGTTGTTTCAGGGGAACTAGTTACCTTTCTTTTTTACATATCTGTTTTTGTAAATAAATATTTATATTTTGTTTTTATTATTTTAATTCTTTTACATCTTTATATACATACATACATGTATATATATATAAACGAGACTTGTGAGAAAGAAGCACCAAACAATTATTCAGTCTTTTCAAATGATTATGGATAGATAATAGACACATTTAGCATCTATTTTCTACTGCTCAATGCTCATAAGATATTATGGTCTACTTCCTTACTCTGTTGTATGATGTTATACGCTTGTAGAATATCAAAGTGAGAATGGATCAGAAACTCCTTCACAGTCTGTAAATATCAGTTCAATTATAACTGTCATCCATAGTTTTATTTTTATTTACGAGATGATTGATATAGTGCACTACTTTATAAAACAATTAACATTCTGACTTATTCATGTAGGTACTTAATGTTTACACCGGTTCTCATGGTTTGCTCCATGGTGGCAGTCATCTGAGGCCGTGGTTATTAATAGATTCATCCACCATTGATCCACGAACATCAAGGGAGCTTTCTGCGGTTGTATCTGGCTGTAGTTTAAGGGAAAAGAGAGGTAATTGTTATTGAATCAAAGTTCTAGACTACTAGGGAGAGATAGTTCGACTCATGGACATTTTTCATGGGAACTCTCAATTAAGATTTTCAGAAATTAGATCATCAACCATTAAGTGCAAACAATATGGCCTATCTTGGAAATGCTGTTTTTTTACTGTGATACTGGGTATAAATGAATTTATCATTTGAGACGTCAGATGGTTATTTGAACCAGGAATTCCTTATTTTTTACCTGAATAGTTATCCAATTGACTTGAATATTCTTAAACTTAAAAAAGGGGTATAATACCTTTTTAGTCCCTGAGGTTTGGGTAACAAGTGGATTTCATCTACAGGTTTCAAAATCGTACATTTTCCCCAACTGTTTAGAAAATACTTCAATTTAATCTCTGACATTAGGTTTTTATTATTATTTTTTTGATACAATATGTGGAGGCAGGGGTTCGAACTCATGACCTCTTGATCAGAGATAGGTGTCTTAACCACTTGAGCTATGTTCGGTTTGGTGATAATAGGTTTTTATTACCCAACACAACTTAATAGAAAAGTGACATGGTGTATTTAATAATAAACCTAGTTGATGTGGTGCCCCACCTCCTCTCCTTCCCCTTCTCTGTAGAGCTTGCTGCTACTGTCCGACGTTGCGTGCTGCTGGTGCAAAATACAAAGCAAATAATAGGCTAAATTAGCAATTGTTTTGAAACTGCTAGAACTTCAATGCTTAGAGGGAACATTGATATTAGATACATGCACACCTGGTATGAACAGAAAGTTACTCAGAATAGTTCCATTCGGCCTTTTAAGATTAGGAGGTGGTTGAAATTAGGAGGAAAAGGAAGGAAAATAATTGGAAGTGGTTGCATATCTGTGACGGTCACTAACATCATGACTTGTTTTCTTTGAATCTATAATTTTTTTCCTTCTCCATTGTATTCTATTCTTTATTGTAATTCTTCATTATCCATTTTCTAAGAATCAATGAGCCAGCCACCAATAAAAGAAATCACAACTTTTCAATCCTGAATTGATGGTTATAATGGGATTGGAAGGATTGAGGAAATTTGCCTGGCAGGATCCTGATAATTTGTTAATGTCAAGTTTTTGTTGATAAGCAGCTCCTGCAATTGCTTTAGTCACAAATTTTCAACTGCTTTATTGACTGATTGTATTTGTAGATTCTTGGGGAAACCCTGCCATGTTGGACGCCCCTGTATCTGGAGGGGTTTTAGCTGCTGATGCTGGGAAACTGACGTTCATGGTATAATTTCATTTTAGGCTTCTGCAGCTTCCATATGTATTCTGGTTTCATTAATTTAATTAATCTTCAAGTGTGCAAGATACAGAACGTGGAAATCTTTGTGAGCAATGTAATTGGAAAAAGGAAAGTAATAACACGTGCTTGGCTAGCACTCAACGGTTATAAATATGGGGGTCTTTTGCTAGATTTTAACCTTTAACATAGTTCCAATCTTCTCTTACTAAAGAACAGCTTCTTTTTTCTTACATTGTCTTTTAAGTAATCTGAAGTGGCTGTCGTAACATGATTGATAAAGCACAAGTAATGAACATGGATACTGGTTCACTGTAGGCAGAAGCCGTGGGTGGTTTTTCGTTTATTGCCCCTCTAAATAGGAAACTTCTTGCCTTGGCCATGGAAGATAGGGTCTTGTGGCCAATTTGTCATTTTATAGGAAATGTGATTGGTCAGGAGGAAGCTTCTTGACGACCTTTGGTGGCATTTATAATTTGGATCCTAGGAGTCCTTTTCGTCTTGCTTATTGTTCATTTTCAACTTCCTATTATTTTTTTCATCCTATTGTGACTAAGAGATGACATGTCCTCAGGTCGGCGGTTCTGAGGAAGCCTACCATTCTGCAAAATCCTTATTCCTTTCAATGGGCAAAAGTTCAGTATATTGTGGTGGAGCAGGAAATGGTTCGGTGAGTTTTGTCTCTCCCCTTTTATTTTATTTTTGTAATCTCCAGCGATTAAAGCTTCATGAGTTACATAAATGTTTGTAGCTACAGTGTTTGGAGTCTTGTTGACACTCTAAATGCAGGTGTTTTGCAGTTACTTAATGATCGAGAAAGCTAGTTTCTCAGTTGTACTGTTTATCTCTTTTTTTTTTTTTTTTATGATTCACTTTCAAGATGCCTTGGTGGTGAAATGGTAAGCTCATCTATTTCTAAAAAAGTTGAAGTTTGTTAGCTATGGAATTTGAAACCACCATTTAAAATGGCATGGCTTGCAGTAACTTTCTTTATAATTAATTATCCTCAACATTCTCAAATGATTTACCTAAATTAGTTCTCCACCAAGTATAATTTCCACTTGTGTATCATATGGTAAAATACCATCCTCAGGAGATCTGTTGCTCATTCGTAGAATTGTTTTGTCATAGGCAGCAAAGATCTGCAACAACTTGGCAATGGCTGTGAGTATGCTTGGTGTTTCAGAAGCTTTGGCTCTAGGCCAGTGTCTCGGAATTTCTGCAAATACTCTTACAAAAGTATTCAATTCATCAAGTGCTCGCTGTTGGAGTAGGTATGTATGTTACTCTGATGAGATCATAGTAATATCTGCATCCTTATAGATTCAATTTAAATTCTTAGTTGTTTCGTGATGGCCCAATTAAATTTGAGTAGCGGTAAGTTTGCTGCATGTCAGGAATATACAATTGCTATTGATATTTAGATTCGTATATTTATTTAATCATCGGCCCAGGAACTAGATGCTTGAAGTTCTTCTGTGAAACTTACGAACCGAATATCTCGTATAAAAGATTAAACAATTAATAGTTCCTAGCTATAGGTTGAAGCATTTTGGACATATAAAGAGATCAAATTGTTCAGGTTTTTTCTCTCACGCTAAACCTTCCCTACTAATGTAAGTTTATTAATACTCGGTAATTGCCACATAACAGTGAAAGTTACAATCCAGTTCCTGGTGTGATGGAGGAGGTGCCCTCTGCAAGAAATTATGATGGTGGGTTCGCATCAAAGCTTATGGTTAGTCACTTAGAACCCGTCTTTAAAAGAGAAAAATGACATTAATATTCCTAGCAACAGATGTTTTAGTATTTCCAGTGAGTTTATGGCTCAAAGAATGCTTCATTTTCATGCTGACGCTCCTTCCCATATGGAGTCCGCAACCCAAAAGCAACCATTGAATTAACTGTATCTTCTTTGTGTTAGTGTCTAAGCTGCACCATTAAAAACCGTACGTGAGATGAGTTTCGCTGGATTTTTTTTGATATTTTCCTCCCTGCTTCTGGGGGAGGGGAGACTAAATCACCTTTTGCTATAACCAAATCCAGAAAATAAAAAATTGGAAAGTATTTCTAATTAAATACCATTTTTCACCGTTCTAAAGCTCGTACTAAACAAACCTTAGTTCACCGTTTCTTCGAGTATCTTTGAAAGTCTTTTACTGATACTATAATATGATTGTTCCAGCATAAGATATTATTTGGCAAGAGGCATTTTCTCATTTGAGAATTGATGTATAATTTATTGATTGAACGCAGGCTAAAGATCTAAACCTTGCAGCAGCATCTGCTGTTGAGATCGGCCTGAGACATCCACTGACCTCCATGGCACAAGAGATGTAAGCCCCATCTGATCTTGCCTTTCTCTGCTCTGTCCATTTATGATTTCATCTTCTGCTTTCATTTTGCTACAATCTTGGACACAAATCTTTATCCCATATGACAATGCTAGTTATTATGAAATCTTCATCTGTCAGATACAATAAGCTGTGCGAAGATGGATATGAAAACAAGGACTTCTCCTGTGTTTTTCGTCATTTTTACTCGGGCGAGGACGAGTTATAA

mRNA sequence

ATGGCGGTTTCTAGAGTAAGAGCTTCTTTTGCGCAGTACATGATCAAATCTAATTTCAAACATTTTCCGGGTGAGACTGCGACGCCTTCCGTGCGTAGATTCTTCTCTGCGGATGCTTTGGCTTCGTTCCAGAGTGTTGGATTCATTGGACTGGGAAATATGGGATCCAGAATGGCAAATAATCTAATAAAGGCTGGATACAAAATGGTTGTACATGACATAAACCGTGATACCATGAAGAAGTACTCAGATATGGGAGTTTCCACCAAGGAAACACCCTTTGAAGTTGCAGAAGCTTCTGACATTGTCATTACGATGCTACCCTCTTCTTCTCATGTACTTAATGTTTACACCGGTTCTCATGGTTTGCTCCATGGTGGCAGTCATCTGAGGCCGTGGTTATTAATAGATTCATCCACCATTGATCCACGAACATCAAGGGAGCTTTCTGCGGTTGTATCTGGCTGTAGTTTAAGGGAAAAGAGAGATTCTTGGGGAAACCCTGCCATGTTGGACGCCCCTGTATCTGGAGGGGTTTTAGCTGCTGATGCTGGGAAACTGACGTTCATGGTCGGCGGTTCTGAGGAAGCCTACCATTCTGCAAAATCCTTATTCCTTTCAATGGGCAAAAGTTCAGTATATTGTGGTGGAGCAGGAAATGGTTCGGCAGCAAAGATCTGCAACAACTTGGCAATGGCTGTGAGTATGCTTGGTGTTTCAGAAGCTTTGGCTCTAGGCCAGTGTCTCGGAATTTCTGCAAATACTCTTACAAAAGTATTCAATTCATCAAGTGCTCGCTGTTGGAGTAGTGAAAGTTACAATCCAGTTCCTGGTGTGATGGAGGAGGTGCCCTCTGCAAGAAATTATGATGGTGGGTTCGCATCAAAGCTTATGGCTAAAGATCTAAACCTTGCAGCAGCATCTGCTGTTGAGATCGGCCTGAGACATCCACTGACCTCCATGGCACAAGAGATATACAATAAGCTGTGCGAAGATGGATATGAAAACAAGGACTTCTCCTGTGTTTTTCGTCATTTTTACTCGGGCGAGGACGAGTTATAA

Coding sequence (CDS)

ATGGCGGTTTCTAGAGTAAGAGCTTCTTTTGCGCAGTACATGATCAAATCTAATTTCAAACATTTTCCGGGTGAGACTGCGACGCCTTCCGTGCGTAGATTCTTCTCTGCGGATGCTTTGGCTTCGTTCCAGAGTGTTGGATTCATTGGACTGGGAAATATGGGATCCAGAATGGCAAATAATCTAATAAAGGCTGGATACAAAATGGTTGTACATGACATAAACCGTGATACCATGAAGAAGTACTCAGATATGGGAGTTTCCACCAAGGAAACACCCTTTGAAGTTGCAGAAGCTTCTGACATTGTCATTACGATGCTACCCTCTTCTTCTCATGTACTTAATGTTTACACCGGTTCTCATGGTTTGCTCCATGGTGGCAGTCATCTGAGGCCGTGGTTATTAATAGATTCATCCACCATTGATCCACGAACATCAAGGGAGCTTTCTGCGGTTGTATCTGGCTGTAGTTTAAGGGAAAAGAGAGATTCTTGGGGAAACCCTGCCATGTTGGACGCCCCTGTATCTGGAGGGGTTTTAGCTGCTGATGCTGGGAAACTGACGTTCATGGTCGGCGGTTCTGAGGAAGCCTACCATTCTGCAAAATCCTTATTCCTTTCAATGGGCAAAAGTTCAGTATATTGTGGTGGAGCAGGAAATGGTTCGGCAGCAAAGATCTGCAACAACTTGGCAATGGCTGTGAGTATGCTTGGTGTTTCAGAAGCTTTGGCTCTAGGCCAGTGTCTCGGAATTTCTGCAAATACTCTTACAAAAGTATTCAATTCATCAAGTGCTCGCTGTTGGAGTAGTGAAAGTTACAATCCAGTTCCTGGTGTGATGGAGGAGGTGCCCTCTGCAAGAAATTATGATGGTGGGTTCGCATCAAAGCTTATGGCTAAAGATCTAAACCTTGCAGCAGCATCTGCTGTTGAGATCGGCCTGAGACATCCACTGACCTCCATGGCACAAGAGATATACAATAAGCTGTGCGAAGATGGATATGAAAACAAGGACTTCTCCTGTGTTTTTCGTCATTTTTACTCGGGCGAGGACGAGTTATAA

Protein sequence

MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL

Homology

[Close]

BLAST of Moc03g25200 vs. NCBI nr
Match: XP_022140218.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Momordica charantia])

HSP 1 Score: 696.4 bits (1796), Expect = 1.2e-196
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS
Sbjct: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL
Sbjct: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK
Sbjct: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL
Sbjct: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 353

[Close]

BLAST of Moc03g25200 vs. NCBI nr
Match: XP_023520240.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 597.8 bits (1540), Expect = 6.0e-167
Identity = 305/353 (86.40%), Postives = 323/353 (91.50%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MAVSRV  SF QY+ KS+ K F  E+ T S+RRFFSAD+ ASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVGTSFMQYLRKSHVKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           NLIKAGYKMVVHDINR+TM+KYSDMGV+TKETPFEVAEASD+VITMLPS SHVL VY GS
Sbjct: 61  NLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGG HLRPWLLIDSSTIDPRTSRELSA+VS   L+EKRDSW NP MLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAG LTFMVGG EE+Y++AK+LFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQ LGISA TLTKV NSSSARCWSSESYNPVPGVME+VPSA +YDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           DLNLAAASA EIGL HPLTS AQEIY KLCEDGYENKDFSCVFRHFYSG+DEL
Sbjct: 301 DLNLAAASASEIGLEHPLTSQAQEIYKKLCEDGYENKDFSCVFRHFYSGDDEL 353

[Close]

BLAST of Moc03g25200 vs. NCBI nr
Match: XP_023000830.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita maxima])

HSP 1 Score: 597.4 bits (1539), Expect = 7.9e-167
Identity = 305/353 (86.40%), Postives = 323/353 (91.50%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASF Q + KS FK F  E+ T ++RRFFSAD+ ASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           N+IKAGYKMVVHDINR+TM+KYSDMGV+TKETPFEVAEASD+VITMLPS SHVL VY GS
Sbjct: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGG HLRPWLLIDSSTIDPRTSRELSA+VS   L+EKRDSW NP MLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAG LTFMVGG EE+Y++AK+LFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQ LGISA TLTKV NSSSARCWSSESYNPVPGVME+VPSA +YDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           DLNLAAASA EIGL HPLTS AQEIY KLCEDGYENKDFSCVFRHFYSG DEL
Sbjct: 301 DLNLAAASASEIGLEHPLTSQAQEIYKKLCEDGYENKDFSCVFRHFYSGVDEL 353

[Close]

BLAST of Moc03g25200 vs. NCBI nr
Match: XP_022927541.1 (probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita moschata])

HSP 1 Score: 594.7 bits (1532), Expect = 5.1e-166
Identity = 306/354 (86.44%), Postives = 325/354 (91.81%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSN-FKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMA 60
           MAVSRVRASF Q + KS+ FK F  E+ T S+RRFFSAD+ ASFQSVGFIGLGNMGSRMA
Sbjct: 1   MAVSRVRASFMQCLRKSSRFKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMA 60

Query: 61  NNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTG 120
           NNLIKAGYKMVVHDINR+TM+KYSDMGV+TKETPFEVAEASD+VITMLPS SHVL VY G
Sbjct: 61  NNLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120

Query: 121 SHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGV 180
           SHGLLHGG HLRPWLLIDSSTIDPRTSRELSA+VS   L+EKRDSW NP MLDAPVSGGV
Sbjct: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180

Query: 181 LAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAADAG LTFMVGG EE+Y++AK+LFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV
Sbjct: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240

Query: 241 SEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMA 300
           SEALALGQ LGISA TLTKV NSSSARCWSSESYNPVPGVME+VPSA +YDGGFASKLMA
Sbjct: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300

Query: 301 KDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           KDLNLAAASA EIGL HPLTS AQEIY KLCEDGYENKDFSCVFRHFYSG++EL
Sbjct: 301 KDLNLAAASASEIGLEHPLTSQAQEIYKKLCEDGYENKDFSCVFRHFYSGDNEL 354

[Close]

BLAST of Moc03g25200 vs. NCBI nr
Match: XP_008445327.1 (PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucumis melo])

HSP 1 Score: 590.1 bits (1520), Expect = 1.3e-164
Identity = 296/352 (84.09%), Postives = 319/352 (90.62%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MA+ R  ASF QYM KS FK FP  + TPS+RRFFSADALASFQSVGFIGLGNMG+RMAN
Sbjct: 1   MAIFRATASFRQYMSKSRFKSFPVGSETPSIRRFFSADALASFQSVGFIGLGNMGTRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           NLIKAGY MVVHDINR+TMK YSD GV+TKETPFEVAEASD+VITMLPS SHVL+VY GS
Sbjct: 61  NLIKAGYNMVVHDINRNTMKNYSDRGVATKETPFEVAEASDVVITMLPSPSHVLDVYNGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGGSH+RPWLLIDSSTIDP+TSRELSAVVS C L+EKR SW +P MLDAPVSGGVL
Sbjct: 121 HGLLHGGSHIRPWLLIDSSTIDPQTSRELSAVVSSCVLKEKRGSWESPVMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADA  LTFMVGG EEAYH+AK+LFL+MGK SVYCGGAGNGSAAKICNNLA+AVSMLGVS
Sbjct: 181 AADARTLTFMVGGLEEAYHAAKALFLAMGKGSVYCGGAGNGSAAKICNNLALAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQ LGISA TLTKVFNSSSARCWSSESYNPVPGVMEEVPSAR+YDGGFA+KLM K
Sbjct: 241 EALALGQSLGISAKTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARDYDGGFATKLMVK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDE 353
           DLNLA ASA E+GL+HPLTS   EIY KLC+DGYENKDFSCVFRHFYSG++E
Sbjct: 301 DLNLAKASASEVGLQHPLTSQGLEIYEKLCKDGYENKDFSCVFRHFYSGDNE 352

[Close]

BLAST of Moc03g25200 vs. ExPASy Swiss-Prot
Match: Q9SUC0 (Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At4g20930 PE=1 SV=3)

HSP 1 Score: 469.5 bits (1207), Expect = 3.2e-131
Identity = 235/354 (66.38%), Postives = 294/354 (83.05%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALAS-FQSVGFIGLGNMGSRMA 60
           MA+ R +        K+NF        + S+ RF S+   ++ FQ+VGFIGLGNMG RM 
Sbjct: 1   MAIRRAQTLLCLSKFKTNF-------VSGSLHRFSSSSQNSNQFQNVGFIGLGNMGFRMV 60

Query: 61  NNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTG 120
           NNLI+AGYK+ VHDINRD MK +++MGVS++ETP+EVA+ S++VITMLPSSSHV++VYTG
Sbjct: 61  NNLIRAGYKVTVHDINRDVMKMFTEMGVSSRETPYEVAQDSEVVITMLPSSSHVMDVYTG 120

Query: 121 SHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGV 180
           ++GLL G + +RP L IDSSTIDP+T+R++S  VS C+L+EKRD+W  P MLDAPVSGGV
Sbjct: 121 TNGLLLGENDIRPALFIDSSTIDPQTTRKISLAVSNCNLKEKRDNWEKPVMLDAPVSGGV 180

Query: 181 LAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAA+AG LTFMVGG E+AY +A+ +  SMG++S+YCGG+GNGSAAKICNNLAMAVSMLG 
Sbjct: 181 LAAEAGTLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSGNGSAAKICNNLAMAVSMLGT 240

Query: 241 SEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMA 300
           SEALALGQ LGISA+TLT+V N+SS RCWSS++YNPVPGVM+ VPS+R+Y+GGFASKLMA
Sbjct: 241 SEALALGQSLGISASTLTEVLNTSSGRCWSSDAYNPVPGVMKGVPSSRDYNGGFASKLMA 300

Query: 301 KDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           KDLNLAAASA E+G + PL S AQEIY K+CE+G+E KDFSCVFRHFY+G+DE+
Sbjct: 301 KDLNLAAASAEEVGHKSPLISKAQEIYKKMCEEGHETKDFSCVFRHFYNGKDEV 347

[Close]

BLAST of Moc03g25200 vs. ExPASy Swiss-Prot
Match: P31937 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=HIBADH PE=1 SV=2)

HSP 1 Score: 278.9 bits (712), Expect = 8.1e-74
Identity = 152/306 (49.67%), Postives = 201/306 (65.69%), Query Frame = 0


Query: 39  ALASFQSVGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAE 98
           ++AS   VGFIGLGNMG+ MA NL+K GY ++++D+  D  K++ D G     +P +VAE
Sbjct: 35  SVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAE 94

Query: 99  ASDIVITMLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSL 158
            +D +ITMLP+S + +  Y+G++G+L      +  LLIDSSTIDP  S+EL+  V     
Sbjct: 95  KADRIITMLPTSINAIEAYSGANGILK--KVKKGSLLIDSSTIDPAVSKELAKEV----- 154

Query: 159 REKRDSWGNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGA 218
            EK  +      +DAPVSGGV AA +G LTFMVGG E+ + +A+ L   MG + VYCG  
Sbjct: 155 -EKMGA----VFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAV 214

Query: 219 GNGSAAKICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPG 278
           G G AAKICNN+ +A+SM+G +EA+ LG  LG+    L K+ N SS RCWSS++YNPVPG
Sbjct: 215 GTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPG 274

Query: 279 VMEEVPSARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKD 338
           VM+ VPSA NY GGF + LMAKDL LA  SA        L S+A +IY  +C  GY  KD
Sbjct: 275 VMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKD 328

Query: 339 FSCVFR 345
           FS VF+
Sbjct: 335 FSSVFQ 328

[Close]

BLAST of Moc03g25200 vs. ExPASy Swiss-Prot
Match: Q5R5E7 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Pongo abelii OX=9601 GN=HIBADH PE=2 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 8.1e-74
Identity = 152/306 (49.67%), Postives = 201/306 (65.69%), Query Frame = 0


Query: 39  ALASFQSVGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAE 98
           ++AS   VGFIGLGNMG+ MA NL+K GY ++++D+  D  K++ D G     +P +VAE
Sbjct: 35  SVASKTPVGFIGLGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFQDAGEQVVSSPADVAE 94

Query: 99  ASDIVITMLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSL 158
            +D +ITMLP+S + +  Y+G++G+L      +  LLIDSSTIDP  S+EL+  V     
Sbjct: 95  KADRIITMLPTSINAIEAYSGANGILK--KVKKGSLLIDSSTIDPAVSKELAKEV----- 154

Query: 159 REKRDSWGNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGA 218
            EK  +      +DAPVSGGV AA +G LTFMVGG E+ + +A+ L   MG + VYCG  
Sbjct: 155 -EKMGA----VFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLGCMGSNVVYCGAV 214

Query: 219 GNGSAAKICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPG 278
           G G AAKICNN+ +A+SM+G +EA+ LG  LG+    L K+ N SS RCWSS++YNPVPG
Sbjct: 215 GTGQAAKICNNMLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPG 274

Query: 279 VMEEVPSARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKD 338
           VM+ VPSA NY GGF + LMAKDL LA  SA        L S+A +IY  +C  GY  KD
Sbjct: 275 VMDGVPSANNYQGGFGATLMAKDLGLAQDSATSTKSPILLGSLAHQIYRMMCAKGYSKKD 328

Query: 339 FSCVFR 345
           FS VF+
Sbjct: 335 FSSVFQ 328

[Close]

BLAST of Moc03g25200 vs. ExPASy Swiss-Prot
Match: Q2HJD7 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=HIBADH PE=2 SV=1)

HSP 1 Score: 278.5 bits (711), Expect = 1.1e-73
Identity = 153/306 (50.00%), Postives = 200/306 (65.36%), Query Frame = 0


Query: 39  ALASFQSVGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAE 98
           ++AS   VGFIG+GNMG+ MA NL+K GY ++++D+  D  K++ D G     +P +VAE
Sbjct: 35  SMASKTPVGFIGVGNMGNPMAKNLMKHGYPLIIYDVFPDACKEFLDAGEQVVSSPADVAE 94

Query: 99  ASDIVITMLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSL 158
            +D +ITMLP+S + +  Y+G++G+L      +  LLIDSSTIDP  S+EL+  V     
Sbjct: 95  KADRIITMLPTSINAIEAYSGANGILK--KVKKGSLLIDSSTIDPMVSKELAKEV----- 154

Query: 159 REKRDSWGNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGA 218
            EK  +      +DAPVSGGV AA +G LTFMVGG EE + +A+ L   MG + VYCG  
Sbjct: 155 -EKMGA----VFMDAPVSGGVGAARSGNLTFMVGGVEEEFAAAQELLGCMGSNVVYCGAV 214

Query: 219 GNGSAAKICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPG 278
           G G AAKICNNL +A+SM+G +EA+ LG  LG+    L K+ N SS RCWSS++YNPVPG
Sbjct: 215 GTGQAAKICNNLLLAISMIGTAEAMNLGIRLGLDPKLLAKILNMSSGRCWSSDTYNPVPG 274

Query: 279 VMEEVPSARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKD 338
           VM+ VPSA NY GGF + LMAKDL LA  SA        L S A +IY  +C  GY  KD
Sbjct: 275 VMDGVPSANNYQGGFGTTLMAKDLGLAQDSATSTKSPILLGSQAHQIYRMMCAKGYSKKD 328

Query: 339 FSCVFR 345
           FS VF+
Sbjct: 335 FSSVFQ 328

[Close]

BLAST of Moc03g25200 vs. ExPASy Swiss-Prot
Match: Q99L13 (3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Hibadh PE=1 SV=1)

HSP 1 Score: 277.7 bits (709), Expect = 1.8e-73
Identity = 152/312 (48.72%), Postives = 202/312 (64.74%), Query Frame = 0


Query: 39  ALASFQSVGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAE 98
           ++AS   VGFIGLGNMG+ MA NL+K GY ++++D+  D  K++ + G     +P EVAE
Sbjct: 34  SMASKTPVGFIGLGNMGNPMAKNLMKHGYPLILYDVFPDVCKEFKEAGEQVASSPAEVAE 93

Query: 99  ASDIVITMLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSL 158
            +D +ITMLPSS + + VY+G++G+L      +  LLIDSSTIDP  S+EL+  V     
Sbjct: 94  KADRIITMLPSSMNAVEVYSGANGILK--KVKKGSLLIDSSTIDPSVSKELAKEV----- 153

Query: 159 REKRDSWGNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGA 218
            EK  +      +DAPVSGGV AA +G LTFMVGG E+ + +A+ L   MG + VYCG  
Sbjct: 154 -EKMGA----VFMDAPVSGGVGAARSGNLTFMVGGVEDEFAAAQELLECMGSNVVYCGAV 213

Query: 219 GNGSAAKICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPG 278
           G G +AKICNN+ +A+SM+G +EA+ LG   G+    L K+ N SS RCWSS++YNPVPG
Sbjct: 214 GTGQSAKICNNMLLAISMIGTAEAMNLGIRSGLDPKLLAKILNMSSGRCWSSDTYNPVPG 273

Query: 279 VMEEVPSARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKD 338
           VM  VPS+ NY GGF + LMAKDL LA  SA        L S+A +IY  +C  GY  KD
Sbjct: 274 VMHGVPSSNNYQGGFGTTLMAKDLGLAQDSATSTKTPILLGSLAHQIYRMMCSKGYSKKD 333

Query: 339 FSCVFRHFYSGE 351
           FS VF++    E
Sbjct: 334 FSSVFQYLREEE 333

[Close]

BLAST of Moc03g25200 vs. ExPASy TrEMBL
Match: A0A6J1CHH7 (3-hydroxyisobutyrate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC111010940 PE=3 SV=1)

HSP 1 Score: 696.4 bits (1796), Expect = 6.0e-197
Identity = 353/353 (100.00%), Postives = 353/353 (100.00%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS
Sbjct: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL
Sbjct: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK
Sbjct: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL
Sbjct: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 353

[Close]

BLAST of Moc03g25200 vs. ExPASy TrEMBL
Match: A0A6J1KJE2 (3-hydroxyisobutyrate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111495162 PE=3 SV=1)

HSP 1 Score: 597.4 bits (1539), Expect = 3.8e-167
Identity = 305/353 (86.40%), Postives = 323/353 (91.50%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MAVSRVRASF Q + KS FK F  E+ T ++RRFFSAD+ ASFQSVGFIGLGNMGSRMAN
Sbjct: 1   MAVSRVRASFMQCLRKSRFKPFAVESETSTIRRFFSADSSASFQSVGFIGLGNMGSRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           N+IKAGYKMVVHDINR+TM+KYSDMGV+TKETPFEVAEASD+VITMLPS SHVL VY GS
Sbjct: 61  NIIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGG HLRPWLLIDSSTIDPRTSRELSA+VS   L+EKRDSW NP MLDAPVSGGVL
Sbjct: 121 HGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADAG LTFMVGG EE+Y++AK+LFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS
Sbjct: 181 AADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQ LGISA TLTKV NSSSARCWSSESYNPVPGVME+VPSA +YDGGFASKLMAK
Sbjct: 241 EALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMAK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           DLNLAAASA EIGL HPLTS AQEIY KLCEDGYENKDFSCVFRHFYSG DEL
Sbjct: 301 DLNLAAASASEIGLEHPLTSQAQEIYKKLCEDGYENKDFSCVFRHFYSGVDEL 353

[Close]

BLAST of Moc03g25200 vs. ExPASy TrEMBL
Match: A0A6J1EHZ4 (3-hydroxyisobutyrate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111434338 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.5e-166
Identity = 306/354 (86.44%), Postives = 325/354 (91.81%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSN-FKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMA 60
           MAVSRVRASF Q + KS+ FK F  E+ T S+RRFFSAD+ ASFQSVGFIGLGNMGSRMA
Sbjct: 1   MAVSRVRASFMQCLRKSSRFKPFAVESETSSIRRFFSADSSASFQSVGFIGLGNMGSRMA 60

Query: 61  NNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTG 120
           NNLIKAGYKMVVHDINR+TM+KYSDMGV+TKETPFEVAEASD+VITMLPS SHVL VY G
Sbjct: 61  NNLIKAGYKMVVHDINRNTMEKYSDMGVATKETPFEVAEASDVVITMLPSPSHVLEVYNG 120

Query: 121 SHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGV 180
           SHGLLHGG HLRPWLLIDSSTIDPRTSRELSA+VS   L+EKRDSW NP MLDAPVSGGV
Sbjct: 121 SHGLLHGGYHLRPWLLIDSSTIDPRTSRELSAIVSSRILKEKRDSWENPVMLDAPVSGGV 180

Query: 181 LAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAADAG LTFMVGG EE+Y++AK+LFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV
Sbjct: 181 LAADAGTLTFMVGGPEESYNAAKTLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240

Query: 241 SEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMA 300
           SEALALGQ LGISA TLTKV NSSSARCWSSESYNPVPGVME+VPSA +YDGGFASKLMA
Sbjct: 241 SEALALGQSLGISAKTLTKVLNSSSARCWSSESYNPVPGVMEKVPSASDYDGGFASKLMA 300

Query: 301 KDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           KDLNLAAASA EIGL HPLTS AQEIY KLCEDGYENKDFSCVFRHFYSG++EL
Sbjct: 301 KDLNLAAASASEIGLEHPLTSQAQEIYKKLCEDGYENKDFSCVFRHFYSGDNEL 354

[Close]

BLAST of Moc03g25200 vs. ExPASy TrEMBL
Match: A0A1S3BBY5 (3-hydroxyisobutyrate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488390 PE=3 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 6.1e-165
Identity = 296/352 (84.09%), Postives = 319/352 (90.62%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MA+ R  ASF QYM KS FK FP  + TPS+RRFFSADALASFQSVGFIGLGNMG+RMAN
Sbjct: 1   MAIFRATASFRQYMSKSRFKSFPVGSETPSIRRFFSADALASFQSVGFIGLGNMGTRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           NLIKAGY MVVHDINR+TMK YSD GV+TKETPFEVAEASD+VITMLPS SHVL+VY GS
Sbjct: 61  NLIKAGYNMVVHDINRNTMKNYSDRGVATKETPFEVAEASDVVITMLPSPSHVLDVYNGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGGSH+RPWLLIDSSTIDP+TSRELSAVVS C L+EKR SW +P MLDAPVSGGVL
Sbjct: 121 HGLLHGGSHIRPWLLIDSSTIDPQTSRELSAVVSSCVLKEKRGSWESPVMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADA  LTFMVGG EEAYH+AK+LFL+MGK SVYCGGAGNGSAAKICNNLA+AVSMLGVS
Sbjct: 181 AADARTLTFMVGGLEEAYHAAKALFLAMGKGSVYCGGAGNGSAAKICNNLALAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQ LGISA TLTKVFNSSSARCWSSESYNPVPGVMEEVPSAR+YDGGFA+KLM K
Sbjct: 241 EALALGQSLGISAKTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARDYDGGFATKLMVK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDE 353
           DLNLA ASA E+GL+HPLTS   EIY KLC+DGYENKDFSCVFRHFYSG++E
Sbjct: 301 DLNLAKASASEVGLQHPLTSQGLEIYEKLCKDGYENKDFSCVFRHFYSGDNE 352

[Close]

BLAST of Moc03g25200 vs. ExPASy TrEMBL
Match: A0A0A0M152 (3-hydroxyisobutyrate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_1G538190 PE=3 SV=1)

HSP 1 Score: 588.2 bits (1515), Expect = 2.3e-164
Identity = 296/353 (83.85%), Postives = 319/353 (90.37%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALASFQSVGFIGLGNMGSRMAN 60
           MA+ R  ASF  YM KS FK FP    TPS+RRFFSAD+LASFQS+GFIGLGNMG+RMAN
Sbjct: 1   MAIFRAAASFRHYMSKSCFKSFPVGNETPSIRRFFSADSLASFQSIGFIGLGNMGTRMAN 60

Query: 61  NLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTGS 120
           NLIKAGY MVVHDINR+TMK YSD+GV+TKETPFEVAEASD+VITMLPS SHVL+VY GS
Sbjct: 61  NLIKAGYNMVVHDINRNTMKNYSDLGVATKETPFEVAEASDVVITMLPSPSHVLDVYNGS 120

Query: 121 HGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVL 180
           HGLLHGG H+RPWLLIDSSTIDP+TSRELSAVVS   L+EKR SW +P MLDAPVSGGVL
Sbjct: 121 HGLLHGGCHIRPWLLIDSSTIDPQTSRELSAVVSSRILKEKRGSWESPVMLDAPVSGGVL 180

Query: 181 AADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVS 240
           AADA  LTFMVGG EEAYH+AK+LFLSMGK SVYCGG+GNGSAAKICNNLA+AVSMLGVS
Sbjct: 181 AADARTLTFMVGGLEEAYHAAKALFLSMGKGSVYCGGSGNGSAAKICNNLALAVSMLGVS 240

Query: 241 EALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAK 300
           EALALGQ LGISA TLTKVFNSSSARCWSSESYNPVPGVMEEVPSAR+YDGGFA+KLM K
Sbjct: 241 EALALGQSLGISAKTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARDYDGGFATKLMFK 300

Query: 301 DLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           DLNLA ASA E+GL+HPLTS AQEIY KLCEDGYENKDFSCVFRHFYSG+DEL
Sbjct: 301 DLNLAKASAGEVGLQHPLTSQAQEIYKKLCEDGYENKDFSCVFRHFYSGDDEL 353

[Close]

BLAST of Moc03g25200 vs. TAIR 10
Match: AT4G20930.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 469.5 bits (1207), Expect = 2.3e-132
Identity = 235/354 (66.38%), Postives = 294/354 (83.05%), Query Frame = 0


Query: 1   MAVSRVRASFAQYMIKSNFKHFPGETATPSVRRFFSADALAS-FQSVGFIGLGNMGSRMA 60
           MA+ R +        K+NF        + S+ RF S+   ++ FQ+VGFIGLGNMG RM 
Sbjct: 1   MAIRRAQTLLCLSKFKTNF-------VSGSLHRFSSSSQNSNQFQNVGFIGLGNMGFRMV 60

Query: 61  NNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYTG 120
           NNLI+AGYK+ VHDINRD MK +++MGVS++ETP+EVA+ S++VITMLPSSSHV++VYTG
Sbjct: 61  NNLIRAGYKVTVHDINRDVMKMFTEMGVSSRETPYEVAQDSEVVITMLPSSSHVMDVYTG 120

Query: 121 SHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGV 180
           ++GLL G + +RP L IDSSTIDP+T+R++S  VS C+L+EKRD+W  P MLDAPVSGGV
Sbjct: 121 TNGLLLGENDIRPALFIDSSTIDPQTTRKISLAVSNCNLKEKRDNWEKPVMLDAPVSGGV 180

Query: 181 LAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGV 240
           LAA+AG LTFMVGG E+AY +A+ +  SMG++S+YCGG+GNGSAAKICNNLAMAVSMLG 
Sbjct: 181 LAAEAGTLTFMVGGPEDAYLAARPILQSMGRTSIYCGGSGNGSAAKICNNLAMAVSMLGT 240

Query: 241 SEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMA 300
           SEALALGQ LGISA+TLT+V N+SS RCWSS++YNPVPGVM+ VPS+R+Y+GGFASKLMA
Sbjct: 241 SEALALGQSLGISASTLTEVLNTSSGRCWSSDAYNPVPGVMKGVPSSRDYNGGFASKLMA 300

Query: 301 KDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCVFRHFYSGEDEL 354
           KDLNLAAASA E+G + PL S AQEIY K+CE+G+E KDFSCVFRHFY+G+DE+
Sbjct: 301 KDLNLAAASAEEVGHKSPLISKAQEIYKKMCEEGHETKDFSCVFRHFYNGKDEV 347

[Close]

BLAST of Moc03g25200 vs. TAIR 10
Match: AT4G29120.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 118.6 bits (296), Expect = 1.0e-26
Identity = 97/327 (29.66%), Postives = 150/327 (45.87%), Query Frame = 0


Query: 20  KHFPGETATPS--VRRFFSADALA------SFQSVGFIGLGNMGSRMANNLIKAGYKMVV 79
           + FP  +   S  +RR  ++  ++      S   +G+IG G MG  M  +LIKAGY + V
Sbjct: 6   RRFPSPSVVSSFFLRRSMASSTISSDIITPSNTKIGWIGTGVMGRSMCGHLIKAGYTVTV 65

Query: 80  HDINRDTMKKYSDMGVSTKETPFEVAEASDIVITMLPSSSHVLNVYT-----GSHGLLHG 139
            +      +   DMG +  ++P  VAE SD+V T++   S V +V          GL  G
Sbjct: 66  FNRTISKAQTLIDMGANVADSPNSVAEQSDVVFTIVGYPSDVRHVLLDPKSGALSGLRQG 125

Query: 140 GSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSWGNPAMLDAPVSGGVLAADAGK 199
           G      +L+D +T +P  + E++          K  S+ N   +DAPVSGG L A  GK
Sbjct: 126 G------VLVDMTTSEPSLAEEIA----------KAASFKNCFSIDAPVSGGDLGAKNGK 185

Query: 200 LTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAKICNNLAMAVSMLGVSEALALG 259
           L+   GG E        LF  MGK + + G +G G  AK+ N + +A +MLG+ E L   
Sbjct: 186 LSIFAGGDETTVKRLDPLFSLMGKVN-FMGTSGKGQFAKLANQITIASTMLGLVEGLIYA 245

Query: 260 QCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPSARNYDGGFASKLMAKDLNLAA 319
              G+      +  ++ +A   S + Y        +    R++D GF      KDL +  
Sbjct: 246 HKAGLDVKKFLEAISTGAAGSKSIDLYG-------DRILKRDFDPGFYVNHFVKDLGICL 305

Query: 320 ASAVEIGLRHPLTSMAQEIYNKLCEDG 334
                +GL  P  ++AQ++Y  L   G
Sbjct: 306 NECQRMGLALPGLALAQQLYLSLKAHG 308

[Close]

BLAST of Moc03g25200 vs. TAIR 10
Match: AT1G71170.1 (6-phosphogluconate dehydrogenase family protein )

HSP 1 Score: 112.1 bits (279), Expect = 9.3e-25
Identity = 82/289 (28.37%), Postives = 146/289 (50.52%), Query Frame = 0


Query: 46  VGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVIT 105
           +G+IG+G MGS M ++++ AGY + V+  +    K     G  T  +P E+ E SD+V T
Sbjct: 16  IGWIGIGIMGSAMVSHILAAGYSVTVYARDLRKTKDLQTKGGRTANSPKELGEMSDVVFT 75

Query: 106 MLPSSSHVLNVYTGSHGLLHGGSHLRP-WLLIDSSTIDPRTSRELSAVVSGCSLREKRDS 165
           ++ +S+ V ++  G  G+L G   L+P  + +D ++  P  +RE+ A         +RD 
Sbjct: 76  IVGNSNDVRSLLLGDDGVLSG---LKPGGVTVDMTSSKPGLAREIYAEA------RRRDC 135

Query: 166 WGNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAA 225
           W     +DAPVSGG   A  GKLT   GG  E       +  +MG    + GGAG+G + 
Sbjct: 136 WA----VDAPVSGGDAGAREGKLTIFAGGDSEIVEWLAPVMKTMGIVR-FMGGAGSGQSC 195

Query: 226 KICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVP 285
           KI N + +  +M+G++E +   +  G+      +     +A        + V  +  E+ 
Sbjct: 196 KIGNQICVGSNMIGLAEGIVFAEKAGLDPVKWLEAVKDGAAG-------SAVMRLFGEMM 255

Query: 286 SARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDG 334
           + R+Y     ++ M KDL +AA +A+ +    P T++ ++++  +  +G
Sbjct: 256 AVRDYKATGFAEYMVKDLGMAAEAAMAM----PGTALNKQLFTVMVANG 279

[Close]

BLAST of Moc03g25200 vs. TAIR 10
Match: AT3G25530.1 (glyoxylate reductase 1 )

HSP 1 Score: 108.6 bits (270), Expect = 1.0e-23
Identity = 86/297 (28.96%), Postives = 133/297 (44.78%), Query Frame = 0


Query: 46  VGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVIT 105
           VGF+GLG MG  M+ NL+K G+K+ V +       +  + G S  E+P EV +     I 
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 106 MLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSW 165
           ML      L+V     G+L      + +  ID ST+D  TS +++  ++G   R      
Sbjct: 63  MLSDPCAALSVVFDKGGVLEQICEGKGY--IDMSTVDAETSLKINEAITGKGGR------ 122

Query: 166 GNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAK 225
                ++ PVSG    A+ G+L  +  G +  +  +   F  +GK S Y G  GNG+  K
Sbjct: 123 ----FVEGPVSGSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMK 182

Query: 226 ICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPS 285
           +  N+ M   M   SE L L    G+S++TL  + +  +         NP+        +
Sbjct: 183 LIVNMIMGSMMNAFSEGLVLADKSGLSSDTLLDILDLGAMT-------NPMFKGKGPSMN 242

Query: 286 ARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCV 343
             +Y   F  K   KD+ LA A   E  +  P+ + A E + K    G  + DFS V
Sbjct: 243 KSSYPPAFPLKHQQKDMRLALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAV 280

[Close]

BLAST of Moc03g25200 vs. TAIR 10
Match: AT3G25530.2 (glyoxylate reductase 1 )

HSP 1 Score: 105.5 bits (262), Expect = 8.7e-23
Identity = 84/297 (28.28%), Postives = 129/297 (43.43%), Query Frame = 0


Query: 46  VGFIGLGNMGSRMANNLIKAGYKMVVHDINRDTMKKYSDMGVSTKETPFEVAEASDIVIT 105
           VGF+GLG MG  M+ NL+K G+K+ V +       +  + G S  E+P EV +     I 
Sbjct: 3   VGFLGLGIMGKAMSMNLLKNGFKVTVWNRTLSKCDELVEHGASVCESPAEVIKKCKYTIA 62

Query: 106 MLPSSSHVLNVYTGSHGLLHGGSHLRPWLLIDSSTIDPRTSRELSAVVSGCSLREKRDSW 165
           ML      L++  G                ID ST+D  TS +++  ++G   R      
Sbjct: 63  MLSDPCAALSICEGKG-------------YIDMSTVDAETSLKINEAITGKGGR------ 122

Query: 166 GNPAMLDAPVSGGVLAADAGKLTFMVGGSEEAYHSAKSLFLSMGKSSVYCGGAGNGSAAK 225
                ++ PVSG    A+ G+L  +  G +  +  +   F  +GK S Y G  GNG+  K
Sbjct: 123 ----FVEGPVSGSKKPAEDGQLIILAAGDKALFEESIPAFDVLGKRSFYLGQVGNGAKMK 182

Query: 226 ICNNLAMAVSMLGVSEALALGQCLGISANTLTKVFNSSSARCWSSESYNPVPGVMEEVPS 285
           +  N+ M   M   SE L L    G+S++TL  + +  +         NP+        +
Sbjct: 183 LIVNMIMGSMMNAFSEGLVLADKSGLSSDTLLDILDLGAMT-------NPMFKGKGPSMN 242

Query: 286 ARNYDGGFASKLMAKDLNLAAASAVEIGLRHPLTSMAQEIYNKLCEDGYENKDFSCV 343
             +Y   F  K   KD+ LA A   E  +  P+ + A E + K    G  + DFS V
Sbjct: 243 KSSYPPAFPLKHQQKDMRLALALGDENAVSMPVAAAANEAFKKARSLGLGDLDFSAV 269

The following BLAST results are available for this feature:

BLAST of Moc03g25200 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022140218.1	1.2e-196	100.00	probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Momordica...	[more]
XP_023520240.1	6.0e-167	86.40	probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita...	[more]
XP_023000830.1	7.9e-167	86.40	probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita...	[more]
XP_022927541.1	5.1e-166	86.44	probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1 [Cucurbita...	[more]
XP_008445327.1	1.3e-164	84.09	PREDICTED: probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial isoform X1...	[more]

BLAST of Moc03g25200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SUC0	3.2e-131	66.38	Probable 3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Arabidopsis thalia...	[more]
P31937	8.1e-74	49.67	3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Homo sapiens OX=9606 GN=HIB...	[more]
Q5R5E7	8.1e-74	49.67	3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Pongo abelii OX=9601 GN=HIB...	[more]
Q2HJD7	1.1e-73	50.00	3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Bos taurus OX=9913 GN=HIBAD...	[more]
Q99L13	1.8e-73	48.72	3-hydroxyisobutyrate dehydrogenase, mitochondrial OS=Mus musculus OX=10090 GN=Hi...	[more]

BLAST of Moc03g25200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CHH7	6.0e-197	100.00	3-hydroxyisobutyrate dehydrogenase OS=Momordica charantia OX=3673 GN=LOC11101094...	[more]
A0A6J1KJE2	3.8e-167	86.40	3-hydroxyisobutyrate dehydrogenase OS=Cucurbita maxima OX=3661 GN=LOC111495162 P...	[more]
A0A6J1EHZ4	2.5e-166	86.44	3-hydroxyisobutyrate dehydrogenase OS=Cucurbita moschata OX=3662 GN=LOC111434338...	[more]
A0A1S3BBY5	6.1e-165	84.09	3-hydroxyisobutyrate dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103488390 PE=3 ...	[more]
A0A0A0M152	2.3e-164	83.85	3-hydroxyisobutyrate dehydrogenase OS=Cucumis sativus OX=3659 GN=Csa_1G538190 PE...	[more]

BLAST of Moc03g25200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20930.1	2.3e-132	66.38	6-phosphogluconate dehydrogenase family protein	[more]
AT4G29120.1	1.0e-26	29.66	6-phosphogluconate dehydrogenase family protein	[more]
AT1G71170.1	9.3e-25	28.37	6-phosphogluconate dehydrogenase family protein	[more]
AT3G25530.1	1.0e-23	28.96	glyoxylate reductase 1	[more]
AT3G25530.2	8.7e-23	28.28	glyoxylate reductase 1	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR011548	3-hydroxyisobutyrate dehydrogenase	TIGRFAM	TIGR01692	TIGR01692	coord: 48..345 e-value: 5.6E-103 score: 342.2
IPR006115	6-phosphogluconate dehydrogenase, NADP-binding	PFAM	PF03446	NAD_binding_2	coord: 46..216 e-value: 1.6E-37 score: 129.2
IPR013328	6-phosphogluconate dehydrogenase, domain 2	GENE3D	1.10.1040.10		coord: 218..351 e-value: 4.8E-45 score: 154.9
None	No IPR available	GENE3D	3.40.50.720		coord: 39..217 e-value: 1.1E-51 score: 177.0
None	No IPR available	PIRSR	PIRSR000103-1	PIRSR000103-1	coord: 45..331 e-value: 7.9E-77 score: 255.8
None	No IPR available	PANTHER	PTHR22981:SF7	3-HYDROXYISOBUTYRATE DEHYDROGENASE, MITOCHONDRIAL	coord: 37..351
None	No IPR available	PANTHER	PTHR22981	3-HYDROXYISOBUTYRATE DEHYDROGENASE-RELATED	coord: 37..351
IPR029154	3-hydroxyisobutyrate dehydrogenase, NAD-binding domain	PFAM	PF14833	NAD_binding_11	coord: 219..345 e-value: 1.1E-29 score: 103.1
IPR002204	3-hydroxyisobutyrate dehydrogenase-related, conserved site	PROSITE	PS00895	3_HYDROXYISOBUT_DH	coord: 48..61
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 45..217
IPR008927	6-phosphogluconate dehydrogenase-like, C-terminal domain superfamily	SUPERFAMILY	48179	6-phosphogluconate dehydrogenase C-terminal domain-like	coord: 218..346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g25200.1	Moc03g25200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006551	leucine metabolic process
biological_process	GO:0006574	valine catabolic process
molecular_function	GO:0008442	3-hydroxyisobutyrate dehydrogenase activity
molecular_function	GO:0051287	NAD binding
molecular_function	GO:0050661	NADP binding
molecular_function	GO:0043621	protein self-association
molecular_function	GO:0016491	oxidoreductase activity