Moc03g25060 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g25060 vs. NCBI nr
Match: XP_022140178.1 (berberine bridge enzyme-like 8 [Momordica charantia])

HSP 1 Score: 1049.7 bits (2713), Expect = 1.3e-302
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH
Sbjct: 30  MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 89

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET
Sbjct: 90  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 149

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD
Sbjct: 150 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 209

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL
Sbjct: 210 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 269

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM
Sbjct: 270 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 329

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 360
           NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP
Sbjct: 330 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 389

Query: 361 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 420
           ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN
Sbjct: 390 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 449

Query: 421 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGN 480
           ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGN
Sbjct: 450 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGN 509

Query: 481 FERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQ 519
           FERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQ
Sbjct: 510 FERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQ 547

BLAST of Moc03g25060 vs. NCBI nr
Match: XP_038895849.1 (berberine bridge enzyme-like 8 [Benincasa hispida])

HSP 1 Score: 891.0 bits (2301), Expect = 7.7e-255
Identity = 440/517 (85.11%), Postives = 473/517 (91.49%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNST+YSKHS+PISEVVFTNESASFSSLL+LSIRNLRFLT + PKP FLVTPF  SH
Sbjct: 32  LECFNSTSYSKHSIPISEVVFTNESASFSSLLQLSIRNLRFLTTTMPKPLFLVTPFHESH 91

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAA+VCA+KK LQVRVRSGGHDYEGLSYVSSQ PFIVIDLINL SI IDIETET SVET
Sbjct: 92  VQAAIVCARKKGLQVRVRSGGHDYEGLSYVSSQAPFIVIDLINLHSITIDIETETTSVET 151

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GATLGE+YY+IAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD
Sbjct: 152 GATLGELYYKIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 211

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRI DRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL+QGATHL
Sbjct: 212 FNGRIMDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHL 271

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQ IAHK+DQD+FLHVTTKV+        +  +S KTI+LSFTSLFLGPIERLIP+M
Sbjct: 272 FQKWQTIAHKLDQDIFLHVTTKVI--------TNFSSKKTIRLSFTSLFLGPIERLIPIM 331

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKSDYVTS 360
            THF ELGLKRNDCTEMSWIQSVLFFAD S ETP E+LMER SPPISD FFTAKSDYVTS
Sbjct: 332 KTHFSELGLKRNDCTEMSWIQSVLFFADFSIETPLEVLMERSSPPISDAFFTAKSDYVTS 391

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PISQ GLE LW+KLLEEEKSELILTPYGGKM QISES+TPFPHRRG+I+GIQYLA W+NA
Sbjct: 392 PISQNGLEGLWTKLLEEEKSELILTPYGGKMSQISESQTPFPHRRGSIFGIQYLATWDNA 451

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NETEKHLSW+REVY+YM+P+VSK PR AYLNYRDLDLGRNY +NTSY+EAKVWGLKYF  
Sbjct: 452 NETEKHLSWIREVYTYMEPYVSKSPRAAYLNYRDLDLGRNYRKNTSYEEAKVWGLKYFSD 511

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRI 517
           NFERLVRVKTKVDPLNFFWNEQSIPL +H+E++ TRI
Sbjct: 512 NFERLVRVKTKVDPLNFFWNEQSIPLLYHYEDD-TRI 539

BLAST of Moc03g25060 vs. NCBI nr
Match: XP_008445632.1 (PREDICTED: flavin-dependent oxidoreductase FOX5-like [Cucumis melo] >KAA0047127.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa] >TYK14944.1 flavin-dependent oxidoreductase FOX5-like [Cucumis melo var. makuwa])

HSP 1 Score: 856.3 bits (2211), Expect = 2.1e-244
Identity = 425/517 (82.21%), Postives = 460/517 (88.97%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNST+YSKHS+P+SEVVFTNESASFSSL RLSIRNLRFLT + PKP FLVTPF  SH
Sbjct: 34  LECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIRNLRFLTTTLPKPLFLVTPFHESH 93

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAA+VCA++K LQVRVRSGGHDYEGLSYVSSQ PFIVIDLINL SI IDIE ETASV T
Sbjct: 94  VQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQAPFIVIDLINLRSITIDIEDETASVGT 153

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GA LGE+YYRIA+KS IHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKI+D
Sbjct: 154 GAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIID 213

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRI DR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL+QGATHL
Sbjct: 214 FNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHL 273

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQ I+HK+DQD+FLHVTTKVV        +   S KTI+LSFTSLFLGPIERLIP+M
Sbjct: 274 FQKWQNISHKLDQDIFLHVTTKVV--------TDFPSKKTIRLSFTSLFLGPIERLIPIM 333

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKSDYVTS 360
            T F ELGLKR+DC EMSWIQSVLFFAD S +TP E+LMER SP ISD FFTAKSDYV S
Sbjct: 334 KTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQISDAFFTAKSDYVIS 393

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PISQ GLE LW KLLEEEKSELILTPYGGKM QISES+TPFPHR G I+GIQYLA W+NA
Sbjct: 394 PISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHREGRIFGIQYLATWDNA 453

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NETEKHLSW+REVY+YM+P+VSK PR AYLNYRDLDLGRNY RNTSY+EAKVWGLKYF  
Sbjct: 454 NETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSD 513

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRI 517
           NFERLVRVKTKVDP NFFWNEQSIPL +H+E++ TRI
Sbjct: 514 NFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD-TRI 541

BLAST of Moc03g25060 vs. NCBI nr
Match: KAE8653305.1 (hypothetical protein Csa_023319 [Cucumis sativus])

HSP 1 Score: 845.1 bits (2182), Expect = 4.9e-241
Identity = 414/513 (80.70%), Postives = 456/513 (88.89%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNST+YSKHS+PISEVVFTNESASFSSLLRLSIRNLRFLT + PKP  LVTPF  SH
Sbjct: 35  LECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPLLLVTPFHESH 94

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAA++CA++K LQVRVRSGGHDYEGLSY+SSQ PFIVIDLINL SI IDI+TETASVET
Sbjct: 95  VQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVIDLINLRSIKIDIKTETASVET 154

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GA+LGE+YYRIA+KS IHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKI+D
Sbjct: 155 GASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIID 214

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRI DR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL++GATHL
Sbjct: 215 FNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEEGATHL 274

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQ I+HK+DQD+FLHVTTKVV        +   S KTI+LSFTSLFLGPIERLIP+M
Sbjct: 275 FQKWQNISHKLDQDIFLHVTTKVV--------TNFPSKKTIRLSFTSLFLGPIERLIPIM 334

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKSDYVTS 360
            T F ELGLKRNDC EMSWIQSVLFFAD S + P E+LM+R SP ISD FFTAKSDYVTS
Sbjct: 335 KTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRSSPQISDAFFTAKSDYVTS 394

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PIS+ GLE LWSKLLEE+KSELI TPYGGKM QISES+ PFPHR G I+GIQYLA W+NA
Sbjct: 395 PISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPHREGRIFGIQYLATWDNA 454

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NE EKHLSW+REVY+YM+ +VSK PR AYLNYRDLDLG NY RNTSY+EAKVWGLKYF  
Sbjct: 455 NENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGRNTSYEEAKVWGLKYFSD 514

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEEN 513
           NF+RLVRVKTKVDP NFFWNEQSIPL +H+E++
Sbjct: 515 NFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDD 539

BLAST of Moc03g25060 vs. NCBI nr
Match: XP_004135724.1 (berberine bridge enzyme-like 13 [Cucumis sativus])

HSP 1 Score: 845.1 bits (2182), Expect = 4.9e-241
Identity = 414/513 (80.70%), Postives = 456/513 (88.89%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNST+YSKHS+PISEVVFTNESASFSSLLRLSIRNLRFLT + PKP  LVTPF  SH
Sbjct: 33  LECFNSTSYSKHSIPISEVVFTNESASFSSLLRLSIRNLRFLTTTLPKPLLLVTPFHESH 92

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAA++CA++K LQVRVRSGGHDYEGLSY+SSQ PFIVIDLINL SI IDI+TETASVET
Sbjct: 93  VQAAILCAREKGLQVRVRSGGHDYEGLSYISSQAPFIVIDLINLRSIKIDIKTETASVET 152

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GA+LGE+YYRIA+KS IHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKI+D
Sbjct: 153 GASLGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIID 212

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRI DR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL++GATHL
Sbjct: 213 FNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEEGATHL 272

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQ I+HK+DQD+FLHVTTKVV        +   S KTI+LSFTSLFLGPIERLIP+M
Sbjct: 273 FQKWQNISHKLDQDIFLHVTTKVV--------TNFPSKKTIRLSFTSLFLGPIERLIPIM 332

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKSDYVTS 360
            T F ELGLKRNDC EMSWIQSVLFFAD S + P E+LM+R SP ISD FFTAKSDYVTS
Sbjct: 333 KTRFSELGLKRNDCIEMSWIQSVLFFADFSIDAPLEVLMDRSSPQISDAFFTAKSDYVTS 392

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PIS+ GLE LWSKLLEE+KSELI TPYGGKM QISES+ PFPHR G I+GIQYLA W+NA
Sbjct: 393 PISENGLEGLWSKLLEEDKSELIFTPYGGKMSQISESQIPFPHREGRIFGIQYLATWDNA 452

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NE EKHLSW+REVY+YM+ +VSK PR AYLNYRDLDLG NY RNTSY+EAKVWGLKYF  
Sbjct: 453 NENEKHLSWIREVYAYMESYVSKSPRAAYLNYRDLDLGTNYGRNTSYEEAKVWGLKYFSD 512

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEEN 513
           NF+RLVRVKTKVDP NFFWNEQSIPL +H+E++
Sbjct: 513 NFKRLVRVKTKVDPSNFFWNEQSIPLLYHYEDD 537

BLAST of Moc03g25060 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 7.7e-149
Identity = 266/499 (53.31%), Postives = 350/499 (70.14%), Query Frame = 0

Query: 10  SKHSVPISEVVFTNE--SASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVC 69
           S  S P+    FT++  ++SF   L  S +NLR+L  S+PKP+F+  P   +HVQAAV+C
Sbjct: 43  SDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLC 102

Query: 70  AKKKSLQVRVRSGGHDYEGLSYVSS-QTPFIVIDLINLSSIHIDIETETASVETGATLGE 129
           AKK  L +R+RSGGHDYEGLSYVS  +T F+++DL  L  I +DIE+ +A V  GA++GE
Sbjct: 103 AKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGE 162

Query: 130 VYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIR 189
           VYYRI EKS IHGFPAG C ++G+GGH+ GG +G++ RK+GL ADNV+DA+IVD +G+I 
Sbjct: 163 VYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKIL 222

Query: 190 DRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQK 249
           +R +MGED+FWAIRGGGG SFGVIL+WK+KLV VP  VTVF V RTL+Q  T L  KWQ+
Sbjct: 223 NRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQ 282

Query: 250 IAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHE 309
           +A K+D+DLF+ V   ++   +K   SKE   +TI  S+   FLG   RL+ +M   F +
Sbjct: 283 VADKLDEDLFIRV---IIQPTSKTPKSKE---RTISTSYQGQFLGDANRLLQVMQRSFPQ 342

Query: 310 LGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGL 369
           LGL + DC E SWI+SV++ A   +  P E L++    +   +F AKSDYV  PI   GL
Sbjct: 343 LGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD-GKSLFKNYFKAKSDYVEEPIPVEGL 402

Query: 370 EALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENANETE-KH 429
           E LW KLLEE+    I  PYGG M +I E+ETPFPHR G ++ IQ+L  W++   +E KH
Sbjct: 403 EGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQDGKTSEAKH 462

Query: 430 LSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLV 489
           + WMRE+YSYM+ +VSK PR AY+NYRDLDLG N + +    +A+ WG +YFKGNFERLV
Sbjct: 463 MGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKGS----DAREWGNRYFKGNFERLV 522

Query: 490 RVKTKVDPLNFFWNEQSIP 505
            +K K DP NFF +EQSIP
Sbjct: 523 EIKAKFDPENFFRHEQSIP 530

BLAST of Moc03g25060 vs. ExPASy Swiss-Prot
Match: Q9SA85 (Berberine bridge enzyme-like 8 OS=Arabidopsis thaliana OX=3702 GN=At1g30700 PE=2 SV=1)

HSP 1 Score: 525.0 bits (1351), Expect = 1.5e-147
Identity = 255/507 (50.30%), Postives = 357/507 (70.41%), Query Frame = 0

Query: 2   ECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHV 61
           +C  S +  KH  PIS  +F + + S+SS+L+ +IRNLRF T S+PKP  ++     SHV
Sbjct: 30  QCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAATHESHV 89

Query: 62  QAAVVCAKKKSLQVRVRSGGHDYEGLSYVS-SQTPFIVIDLINLSSIHIDIETETASVET 121
           QAA+ C K+ +LQ+++RSGGHDY+GLSYV+ S  PF V+D+ NL S+ +D+ ++TA V+T
Sbjct: 90  QAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQT 149

Query: 122 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 181
           GA LGEVYY I EKS    +PAG CPTVGVGGH+SGGG+G + RKYGL  DN IDA++VD
Sbjct: 150 GAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVD 209

Query: 182 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 241
            NG+I DR  MGEDL+WAI GGGG S+GV+L++K+ LV VP +VTVF + RTL+Q AT +
Sbjct: 210 VNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQNATDI 269

Query: 242 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 301
             +WQ++A K+  +LF+     VV       +   +S KT++ +F ++FLG    L+ ++
Sbjct: 270 IHRWQQVAPKLPDELFIRTVIDVV-------NGTVSSQKTVRTTFIAMFLGDTTTLLSIL 329

Query: 302 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 361
           N  F ELGL R+DCTE SWIQSVLF+ ++   +   +L++R+ P++  +   KSDYV  P
Sbjct: 330 NRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQPVN--YLKRKSDYVREP 389

Query: 362 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 421
           IS+TGLE++W K++E E   +   PYGG+M +IS + TPFP+R GN++ IQY A W +  
Sbjct: 390 ISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDET 449

Query: 422 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERN--TSYDEAKVWGLKYFK 481
            T++++   R++Y +M PFVSK PR ++ NYRD+DLG N      +SY E K +G KYF 
Sbjct: 450 LTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFA 509

Query: 482 GNFERLVRVKTKVDPLNFFWNEQSIPL 506
           GNFERLV++KT+VD  NFF NEQSIP+
Sbjct: 510 GNFERLVKIKTRVDSGNFFRNEQSIPV 525

BLAST of Moc03g25060 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 6.8e-145
Identity = 257/496 (51.81%), Postives = 341/496 (68.75%), Query Frame = 0

Query: 13  SVPISEVVFT--NESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKK 72
           S PI+   F+    +  F   L  + +NLR+LT S+PKP F+  P   +HVQAAVVCAKK
Sbjct: 44  SFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKK 103

Query: 73  KSLQVRVRSGGHDYEGLSYVS-SQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYY 132
             L +R+RSGGHDYEGLS+V+  +TPF+++DL  L  + +D+++ +A    GAT+GEVYY
Sbjct: 104 LQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYY 163

Query: 133 RIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRN 192
           RI EKS  HGFPAG C ++G+GGH+ GG +G++ RK+GL ADNV+DA+IVD NG+I DR 
Sbjct: 164 RIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRA 223

Query: 193 SMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAH 252
           +MGED+FWAIRGGGG SFGVIL+WK+KLV VP  VTVF V +TL+Q  T +  KW++IA 
Sbjct: 224 AMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIAD 283

Query: 253 KMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGL 312
           K+D DLF+ V        TK        ++TI +S+ + FLG   RL+ +M   F ELGL
Sbjct: 284 KLDDDLFIRVIISPASKTTK------PGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGL 343

Query: 313 KRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEAL 372
            + DCTEMSWI+SV++ A       PE L+     +    F AKSD+V  PI   GLE L
Sbjct: 344 TKKDCTEMSWIKSVMYIAGFPNSAAPEALL-AGKSLFKNHFKAKSDFVKEPIPVEGLEGL 403

Query: 373 WSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN-ETEKHLSW 432
           W + LEE+    I  PYGG M +ISESE PFPHR G ++ IQ+L+ W++     E+H+ W
Sbjct: 404 WERFLEEDSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKW 463

Query: 433 MREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVK 492
           +RE+YSYM+ +VSK PR AY+NYRDLDLG N E  T   +A+ WG KY+KGNFERLV++K
Sbjct: 464 IREMYSYMEQYVSKNPRQAYVNYRDLDLGTN-EGET---DAREWGAKYYKGNFERLVKIK 523

Query: 493 TKVDPLNFFWNEQSIP 505
            + DP NFF +EQS+P
Sbjct: 524 GEFDPDNFFRHEQSVP 528

BLAST of Moc03g25060 vs. ExPASy Swiss-Prot
Match: Q9FKU9 (Berberine bridge enzyme-like 25 OS=Arabidopsis thaliana OX=3702 GN=At5g44390 PE=2 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 3.7e-143
Identity = 257/497 (51.71%), Postives = 344/497 (69.22%), Query Frame = 0

Query: 16  ISEVVFTNE--SASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSL 75
           + +  FT E  ++ F+ +L  + +N R+LT + PKP F+  P   SHVQA+V+C+KK  +
Sbjct: 51  LDKTFFTPERNASIFTEVLESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEI 110

Query: 76  QVRVRSGGHDYEGLSYVSS-QTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIA 135
             RVRSGGHDYEG+SYVS  + PF++IDL  L  I++DI+  +A VE GAT+GE+YYRIA
Sbjct: 111 HFRVRSGGHDYEGVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIA 170

Query: 136 EKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMG 195
           EKS  HGFPAG  P++G+GGH++GG +G+L RKYGLAADNV+DAKIVD NG++ DR SMG
Sbjct: 171 EKSKFHGFPAGVYPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMG 230

Query: 196 EDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGAT-HLFQKWQKIAHKM 255
           EDLFWAIRGG G SFG+ILSWK+KLV VP  +TVF V +T +Q  +  +  KWQ+IA  +
Sbjct: 231 EDLFWAIRGGSGGSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNL 290

Query: 256 DQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKR 315
             +LFL V   V  +K         ++KT+ +++   FLG    L+ +M   F ELGL +
Sbjct: 291 VDELFLRVFFTVSGNK---------ANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQ 350

Query: 316 NDCTEMSWIQSVLFFADLSTETPP--EILMERSPPISDTFFTAKSDYVTSPISQTGLEAL 375
            DC EMSWI S+++ +   T  PP  EIL++   PI   +F  KSD+   PI   GLE +
Sbjct: 351 KDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGM 410

Query: 376 WSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENANE-TEKHLSW 435
           + KLLEE+ + +I TPYGGKMD+I ESE PFPHR G  + IQY  +W ++ +   +   W
Sbjct: 411 FKKLLEEDAALVIWTPYGGKMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKW 470

Query: 436 MREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTS-YDEAKVWGLKYFKGNFERLVRV 495
           +RE+Y YM P+VS  PR AY+NYRDLDLG+N + + S + EAK+WG  YFK NF RLVR+
Sbjct: 471 IRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRI 530

Query: 496 KTKVDPLNFFWNEQSIP 505
           K+KVDP NFF +EQSIP
Sbjct: 531 KSKVDPDNFFRHEQSIP 538

BLAST of Moc03g25060 vs. ExPASy Swiss-Prot
Match: Q9FKV2 (Berberine bridge enzyme-like 23 OS=Arabidopsis thaliana OX=3702 GN=At5g44360 PE=2 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 6.4e-143
Identity = 254/490 (51.84%), Postives = 343/490 (70.00%), Query Frame = 0

Query: 20  VFTNESASFSSLLRLSIRNLRFLT--NSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRV 79
           V+T+ S S  S      +N RFLT   +S KP  +VTP   + +Q +++C++K  ++VR 
Sbjct: 59  VYTDFSQSLIS------QNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRT 118

Query: 80  RSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPI 139
           +SGGHDYEGLSY+S  +PFI++DL+N+ SI I++  ETA V  GAT+GE+YY+IA+ S I
Sbjct: 119 KSGGHDYEGLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKI 178

Query: 140 HGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRI-RDRNSMGEDLF 199
           HGFPAG+CP+VGVGGH SGGGFG + RK+GLAADNV+DA+ VD NGRI   R  MGEDLF
Sbjct: 179 HGFPAGTCPSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLF 238

Query: 200 WAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLF 259
           WAIRGGG ASFGV+LSWK+KLV VP  VT F  +  L Q  T +  +WQ+IA ++D +LF
Sbjct: 239 WAIRGGGAASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLF 298

Query: 260 LHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTE 319
           + V   +             S  +++ +F + +LG I++LIPLMN  F ELGL   DC+E
Sbjct: 299 IRVIVSI-------------SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSE 358

Query: 320 MSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEE 379
           M+WI S+++F +     P E L++R    +D +F AKSD+V +PI + GLE +W++  E 
Sbjct: 359 MTWIDSIMYF-NWKKGQPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEV 418

Query: 380 EKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAW--ENANETEKHLSWMREVYS 439
           E   +I+ P GGKM +I E+ETPFPHRRGN+Y IQY+  W  ++    EKH++WMR +Y 
Sbjct: 419 ESPIMIMEPLGGKMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYR 478

Query: 440 YMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPL 499
           YM+ +VS  PRGAYLNYRDLDLG N   NTS+++AK+WG +YF  NF+RL  VK K+DP 
Sbjct: 479 YMRVYVSASPRGAYLNYRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPT 528

Query: 500 NFFWNEQSIP 505
           NFF NEQS+P
Sbjct: 539 NFFRNEQSVP 528

BLAST of Moc03g25060 vs. ExPASy TrEMBL
Match: A0A6J1CHF0 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111010911 PE=3 SV=1)

HSP 1 Score: 1049.7 bits (2713), Expect = 6.3e-303
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH
Sbjct: 30  MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 89

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET
Sbjct: 90  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 149

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD
Sbjct: 150 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 209

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL
Sbjct: 210 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 269

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM
Sbjct: 270 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 329

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 360
           NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP
Sbjct: 330 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 389

Query: 361 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 420
           ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN
Sbjct: 390 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 449

Query: 421 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGN 480
           ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGN
Sbjct: 450 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGN 509

Query: 481 FERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQ 519
           FERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQ
Sbjct: 510 FERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQ 547

BLAST of Moc03g25060 vs. ExPASy TrEMBL
Match: A0A5A7TYX1 (Flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold437G00060 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 1.0e-244
Identity = 425/517 (82.21%), Postives = 460/517 (88.97%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNST+YSKHS+P+SEVVFTNESASFSSL RLSIRNLRFLT + PKP FLVTPF  SH
Sbjct: 34  LECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIRNLRFLTTTLPKPLFLVTPFHESH 93

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAA+VCA++K LQVRVRSGGHDYEGLSYVSSQ PFIVIDLINL SI IDIE ETASV T
Sbjct: 94  VQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQAPFIVIDLINLRSITIDIEDETASVGT 153

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GA LGE+YYRIA+KS IHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKI+D
Sbjct: 154 GAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIID 213

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRI DR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL+QGATHL
Sbjct: 214 FNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHL 273

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQ I+HK+DQD+FLHVTTKVV        +   S KTI+LSFTSLFLGPIERLIP+M
Sbjct: 274 FQKWQNISHKLDQDIFLHVTTKVV--------TDFPSKKTIRLSFTSLFLGPIERLIPIM 333

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKSDYVTS 360
            T F ELGLKR+DC EMSWIQSVLFFAD S +TP E+LMER SP ISD FFTAKSDYV S
Sbjct: 334 KTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQISDAFFTAKSDYVIS 393

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PISQ GLE LW KLLEEEKSELILTPYGGKM QISES+TPFPHR G I+GIQYLA W+NA
Sbjct: 394 PISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHREGRIFGIQYLATWDNA 453

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NETEKHLSW+REVY+YM+P+VSK PR AYLNYRDLDLGRNY RNTSY+EAKVWGLKYF  
Sbjct: 454 NETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSD 513

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRI 517
           NFERLVRVKTKVDP NFFWNEQSIPL +H+E++ TRI
Sbjct: 514 NFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD-TRI 541

BLAST of Moc03g25060 vs. ExPASy TrEMBL
Match: A0A1S3BE11 (flavin-dependent oxidoreductase FOX5-like OS=Cucumis melo OX=3656 GN=LOC103488588 PE=3 SV=1)

HSP 1 Score: 856.3 bits (2211), Expect = 1.0e-244
Identity = 425/517 (82.21%), Postives = 460/517 (88.97%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNST+YSKHS+P+SEVVFTNESASFSSL RLSIRNLRFLT + PKP FLVTPF  SH
Sbjct: 34  LECFNSTSYSKHSIPVSEVVFTNESASFSSLFRLSIRNLRFLTTTLPKPLFLVTPFHESH 93

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           VQAA+VCA++K LQVRVRSGGHDYEGLSYVSSQ PFIVIDLINL SI IDIE ETASV T
Sbjct: 94  VQAAIVCAREKGLQVRVRSGGHDYEGLSYVSSQAPFIVIDLINLRSITIDIEDETASVGT 153

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GA LGE+YYRIA+KS IHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKI+D
Sbjct: 154 GAALGELYYRIAKKSSIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIID 213

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
           FNGRI DR+SMGEDLFWAIRGGGGASFGVILSWKLKLVSVP  VTVFNV RTL+QGATHL
Sbjct: 214 FNGRIMDRSSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPSIVTVFNVQRTLEQGATHL 273

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
           FQKWQ I+HK+DQD+FLHVTTKVV        +   S KTI+LSFTSLFLGPIERLIP+M
Sbjct: 274 FQKWQNISHKLDQDIFLHVTTKVV--------TDFPSKKTIRLSFTSLFLGPIERLIPIM 333

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMER-SPPISDTFFTAKSDYVTS 360
            T F ELGLKR+DC EMSWIQSVLFFAD S +TP E+LMER SP ISD FFTAKSDYV S
Sbjct: 334 KTRFSELGLKRSDCIEMSWIQSVLFFADFSIDTPLEVLMERSSPQISDAFFTAKSDYVIS 393

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PISQ GLE LW KLLEEEKSELILTPYGGKM QISES+TPFPHR G I+GIQYLA W+NA
Sbjct: 394 PISQKGLEGLWKKLLEEEKSELILTPYGGKMSQISESQTPFPHREGRIFGIQYLATWDNA 453

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NETEKHLSW+REVY+YM+P+VSK PR AYLNYRDLDLGRNY RNTSY+EAKVWGLKYF  
Sbjct: 454 NETEKHLSWIREVYAYMKPYVSKSPRAAYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFSD 513

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRI 517
           NFERLVRVKTKVDP NFFWNEQSIPL +H+E++ TRI
Sbjct: 514 NFERLVRVKTKVDPSNFFWNEQSIPLLYHYEDD-TRI 541

BLAST of Moc03g25060 vs. ExPASy TrEMBL
Match: A0A2H5N278 (Uncharacterized protein OS=Citrus unshiu OX=55188 GN=CUMW_008660 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 1.3e-236
Identity = 428/791 (54.11%), Postives = 551/791 (69.66%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           ++CF+S    +H    S V+ T  S+++SS+L+ SIRN RFL NS+ KPQF++TP   SH
Sbjct: 32  LQCFSSNL--QHPNEASNVLLTTNSSNYSSVLQSSIRNHRFLNNSTLKPQFIITPSHVSH 91

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVET 120
           +QAA+ C+K+  LQVRVRS GHDYEGLSYV +  PF++IDL NL SI +DIE E+A VE+
Sbjct: 92  IQAAIRCSKQNGLQVRVRSAGHDYEGLSYV-ADVPFLIIDLFNLRSIRVDIENESAWVES 151

Query: 121 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 180
           GATLGE+Y++IAEKS ++GFPAGSC TVGVGGH SGGGFGT+FRKYGLAADN+IDAKIVD
Sbjct: 152 GATLGELYHKIAEKSKLYGFPAGSCSTVGVGGHFSGGGFGTIFRKYGLAADNIIDAKIVD 211

Query: 181 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 240
            NG+I  R SMGEDLFWAIRGGGGASFGVI SWK+K+V VP  VTVF+V  TL+QGA+ L
Sbjct: 212 VNGKILTRKSMGEDLFWAIRGGGGASFGVIFSWKVKIVPVPQTVTVFSVRYTLEQGASKL 271

Query: 241 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 300
            QKWQ +AH++D+D+FLH   +V D  T    S   S+KT+ +SF SL+LG +E+L+ L+
Sbjct: 272 LQKWQNVAHELDEDIFLHAVLEVADSTT----SSAGSNKTVLVSFGSLYLGGVEKLVSLL 331

Query: 301 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 360
              F +LGL R +CTEM+WIQSVL+FA  ST+    +L++RS      F  AKSDY+T P
Sbjct: 332 QESFPQLGLMRENCTEMTWIQSVLYFAGFSTKDSLNVLLDRSTQYKG-FLKAKSDYLTKP 391

Query: 361 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 420
           +S+TGLE L+  LLEEE   LILTPYGG+M +IS+SE  FPHR+GNIY IQYL  W+  +
Sbjct: 392 VSETGLEGLYRILLEEEAPVLILTPYGGRMSEISDSEIAFPHRKGNIYAIQYLTNWDEED 451

Query: 421 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYER-NTSYDEAKVWGLKYFKG 480
           ETEKH+S MR +Y YM+P+VSK PR AYLNYRDLDLGRN    N+SY +A  WGLKYFK 
Sbjct: 452 ETEKHISSMRRLYKYMKPYVSKAPRAAYLNYRDLDLGRNNNAGNSSYAQAYGWGLKYFKN 511

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEENGTRILQSQVQKMSSKNMVQPQLPLFEG 540
           NF+RLVRVKT VDP NFF NEQSIP+  +       +L S   + +S             
Sbjct: 512 NFKRLVRVKTAVDPDNFFRNEQSIPV--YVLSLLLILLLSSASRTNS------------- 571

Query: 541 KNYRWWSQQMKVLYGSQGLWDIVDRGYLVPEGEDGLSQQQLNELRDARKKDNNALFFIYQ 600
                               D V+  ++     +       +   D +   N++   I Q
Sbjct: 572 --------------------DSVEDSFIHCLNSNSDLSVPFSTFCDQK---NSSFGSILQ 631

Query: 601 AVDENIFERISGVSTAKAAWDELQCLFFNTSSPKPLFLVTPFHEFHVQAAIVCANKKGLQ 660
           +  +N+                    +   S PKP F+ TP +E HVQAA++C+ + G+ 
Sbjct: 632 SSAQNL-------------------RYLTPSMPKPEFIFTPLYESHVQAAVICSKQLGIH 691

Query: 661 VRVRSGGHDYEGLSYTSS-QAPFIVIDLIHLRAIDIDIKTETASVETGATLGELYYRIAE 720
           +RVRSGGHDYEGLSY S  + PFI++DL  LRAI +DI++ +A V+TGAT+GE+YYRIAE
Sbjct: 692 LRVRSGGHDYEGLSYVSEIETPFIIVDLARLRAISVDIESNSAWVQTGATIGEVYYRIAE 751

Query: 721 KSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNIIDAKIVDFKGRIMDRNSMGE 780
            S IHGFPAG C ++GVGGH++GG +G++ RKYGL ADN++DA+IVD  G+I+DR +MGE
Sbjct: 752 NSKIHGFPAGLCTSLGVGGHITGGAYGSMMRKYGLGADNVLDARIVDANGKILDREAMGE 757

Query: 781 DLFWAIRGGGG 790
           DLFWAIRGGGG
Sbjct: 812 DLFWAIRGGGG 757

BLAST of Moc03g25060 vs. ExPASy TrEMBL
Match: A0A6J1JUS4 (berberine bridge enzyme-like 15 OS=Cucurbita maxima OX=3661 GN=LOC111489931 PE=3 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 4.3e-235
Identity = 404/512 (78.91%), Postives = 454/512 (88.67%), Query Frame = 0

Query: 1   MECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASH 60
           +ECFNSTTYSKHS+P+SEVVF+NESASFSSLL+ SIRNLRFL+ SSPKP FLVTPF  SH
Sbjct: 34  LECFNSTTYSKHSLPVSEVVFSNESASFSSLLKHSIRNLRFLSTSSPKPLFLVTPFHESH 93

Query: 61  VQAAVVCAKKKSLQVRVRSGGHDYEGLSYV-SSQTPFIVIDLINLSSIHIDIETETASVE 120
           VQAA+VCAK+  LQVRV SGGHDYEGLSY+ SSQ PFIVIDLINL SI I+I+TETA VE
Sbjct: 94  VQAAIVCAKENDLQVRVLSGGHDYEGLSYLSSSQAPFIVIDLINLRSIDINIKTETALVE 153

Query: 121 TGATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIV 180
           TGAT+GE+YYRIA+KSPIHGFPAGSCPTVGVGGH+SGGGFGTLFRKYGLAADNVIDAKIV
Sbjct: 154 TGATIGELYYRIAKKSPIHGFPAGSCPTVGVGGHISGGGFGTLFRKYGLAADNVIDAKIV 213

Query: 181 DFNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATH 240
           DFNGRI DRN+MGEDLFWAIRGGGGASFGVILSWKLKLVS+P  VT FNV RTL+QGATH
Sbjct: 214 DFNGRIMDRNTMGEDLFWAIRGGGGASFGVILSWKLKLVSLPSIVTTFNVQRTLNQGATH 273

Query: 241 LFQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPL 300
           LFQKWQ+IAHK+DQDLFLHVTTKV+D K       + +SKT+ LSFTSLFLG I+ LIPL
Sbjct: 274 LFQKWQQIAHKLDQDLFLHVTTKVIDQK-----MTKTTSKTLSLSFTSLFLGSIDSLIPL 333

Query: 301 MNTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTS 360
           M+THF ELGLKR  CTEM+WIQS+LFFAD ST++P EILM+R P    +F  AKSDYVTS
Sbjct: 334 MDTHFPELGLKRYQCTEMNWIQSILFFADFSTKSPLEILMQRLPSTRSSFI-AKSDYVTS 393

Query: 361 PISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENA 420
           PISQ+GLE LW+KLLE E SELI TPYGGKM QISE ETPFPHR+G+I+G+QYLA W+++
Sbjct: 394 PISQSGLEGLWAKLLENENSELIFTPYGGKMSQISELETPFPHRKGSIFGVQYLATWDDS 453

Query: 421 NETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKG 480
           NE E+HL W+REVYSYM+P+VS  PRG YLNYRDLDLGRNY RNTSY+EAKVWGLKYFKG
Sbjct: 454 NENERHLRWIREVYSYMEPYVSMAPRGTYLNYRDLDLGRNYGRNTSYEEAKVWGLKYFKG 513

Query: 481 NFERLVRVKTKVDPLNFFWNEQSIPLCHHHEE 512
           NFERLV+VKTK DPLNFFWNEQSIPL ++ E+
Sbjct: 514 NFERLVKVKTKFDPLNFFWNEQSIPLLYNCED 539

BLAST of Moc03g25060 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 529.3 bits (1362), Expect = 5.5e-150
Identity = 266/499 (53.31%), Postives = 350/499 (70.14%), Query Frame = 0

Query: 10  SKHSVPISEVVFTNE--SASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVC 69
           S  S P+    FT++  ++SF   L  S +NLR+L  S+PKP+F+  P   +HVQAAV+C
Sbjct: 43  SDASFPMDSSFFTHDLNASSFKLALETSAQNLRYLMPSNPKPEFIFEPLYETHVQAAVLC 102

Query: 70  AKKKSLQVRVRSGGHDYEGLSYVSS-QTPFIVIDLINLSSIHIDIETETASVETGATLGE 129
           AKK  L +R+RSGGHDYEGLSYVS  +T F+++DL  L  I +DIE+ +A V  GA++GE
Sbjct: 103 AKKLKLHLRLRSGGHDYEGLSYVSEMETAFVIVDLSKLRQISVDIESNSAWVHAGASIGE 162

Query: 130 VYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIR 189
           VYYRI EKS IHGFPAG C ++G+GGH+ GG +G++ RK+GL ADNV+DA+IVD +G+I 
Sbjct: 163 VYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMMRKFGLGADNVLDARIVDADGKIL 222

Query: 190 DRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQK 249
           +R +MGED+FWAIRGGGG SFGVIL+WK+KLV VP  VTVF V RTL+Q  T L  KWQ+
Sbjct: 223 NRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEIVTVFTVTRTLEQDGTKLLYKWQQ 282

Query: 250 IAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHE 309
           +A K+D+DLF+ V   ++   +K   SKE   +TI  S+   FLG   RL+ +M   F +
Sbjct: 283 VADKLDEDLFIRV---IIQPTSKTPKSKE---RTISTSYQGQFLGDANRLLQVMQRSFPQ 342

Query: 310 LGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGL 369
           LGL + DC E SWI+SV++ A   +  P E L++    +   +F AKSDYV  PI   GL
Sbjct: 343 LGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLD-GKSLFKNYFKAKSDYVEEPIPVEGL 402

Query: 370 EALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENANETE-KH 429
           E LW KLLEE+    I  PYGG M +I E+ETPFPHR G ++ IQ+L  W++   +E KH
Sbjct: 403 EGLWEKLLEEDSPLTIWNPYGGMMAKIPETETPFPHRSGTLFKIQWLTLWQDGKTSEAKH 462

Query: 430 LSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLV 489
           + WMRE+YSYM+ +VSK PR AY+NYRDLDLG N + +    +A+ WG +YFKGNFERLV
Sbjct: 463 MGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKGS----DAREWGNRYFKGNFERLV 522

Query: 490 RVKTKVDPLNFFWNEQSIP 505
            +K K DP NFF +EQSIP
Sbjct: 523 EIKAKFDPENFFRHEQSIP 530

BLAST of Moc03g25060 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 1.0e-148
Identity = 255/507 (50.30%), Postives = 357/507 (70.41%), Query Frame = 0

Query: 2   ECFNSTTYSKHSVPISEVVFTNESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHV 61
           +C  S +  KH  PIS  +F + + S+SS+L+ +IRNLRF T S+PKP  ++     SHV
Sbjct: 30  QCLTSNSDPKH--PISPAIFFSGNGSYSSVLQANIRNLRFNTTSTPKPFLIIAATHESHV 89

Query: 62  QAAVVCAKKKSLQVRVRSGGHDYEGLSYVS-SQTPFIVIDLINLSSIHIDIETETASVET 121
           QAA+ C K+ +LQ+++RSGGHDY+GLSYV+ S  PF V+D+ NL S+ +D+ ++TA V+T
Sbjct: 90  QAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQT 149

Query: 122 GATLGEVYYRIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVD 181
           GA LGEVYY I EKS    +PAG CPTVGVGGH+SGGG+G + RKYGL  DN IDA++VD
Sbjct: 150 GAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVD 209

Query: 182 FNGRIRDRNSMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHL 241
            NG+I DR  MGEDL+WAI GGGG S+GV+L++K+ LV VP +VTVF + RTL+Q AT +
Sbjct: 210 VNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKINLVEVPENVTVFRISRTLEQNATDI 269

Query: 242 FQKWQKIAHKMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLM 301
             +WQ++A K+  +LF+     VV       +   +S KT++ +F ++FLG    L+ ++
Sbjct: 270 IHRWQQVAPKLPDELFIRTVIDVV-------NGTVSSQKTVRTTFIAMFLGDTTTLLSIL 329

Query: 302 NTHFHELGLKRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSP 361
           N  F ELGL R+DCTE SWIQSVLF+ ++   +   +L++R+ P++  +   KSDYV  P
Sbjct: 330 NRRFPELGLVRSDCTETSWIQSVLFWTNIQVGSSETLLLQRNQPVN--YLKRKSDYVREP 389

Query: 362 ISQTGLEALWSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN 421
           IS+TGLE++W K++E E   +   PYGG+M +IS + TPFP+R GN++ IQY A W +  
Sbjct: 390 ISRTGLESIWKKMIELEIPTMAFNPYGGEMGRISSTVTPFPYRAGNLWKIQYGANWRDET 449

Query: 422 ETEKHLSWMREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERN--TSYDEAKVWGLKYFK 481
            T++++   R++Y +M PFVSK PR ++ NYRD+DLG N      +SY E K +G KYF 
Sbjct: 450 LTDRYMELTRKLYQFMTPFVSKNPRQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFA 509

Query: 482 GNFERLVRVKTKVDPLNFFWNEQSIPL 506
           GNFERLV++KT+VD  NFF NEQSIP+
Sbjct: 510 GNFERLVKIKTRVDSGNFFRNEQSIPV 525

BLAST of Moc03g25060 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 516.2 bits (1328), Expect = 4.8e-146
Identity = 257/496 (51.81%), Postives = 341/496 (68.75%), Query Frame = 0

Query: 13  SVPISEVVFT--NESASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKK 72
           S PI+   F+    +  F   L  + +NLR+LT S+PKP F+  P   +HVQAAVVCAKK
Sbjct: 44  SFPITASFFSPDQNATLFKEELESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKK 103

Query: 73  KSLQVRVRSGGHDYEGLSYVS-SQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYY 132
             L +R+RSGGHDYEGLS+V+  +TPF+++DL  L  + +D+++ +A    GAT+GEVYY
Sbjct: 104 LQLHLRLRSGGHDYEGLSFVAEDETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYY 163

Query: 133 RIAEKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRN 192
           RI EKS  HGFPAG C ++G+GGH+ GG +G++ RK+GL ADNV+DA+IVD NG+I DR 
Sbjct: 164 RIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRA 223

Query: 193 SMGEDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAH 252
           +MGED+FWAIRGGGG SFGVIL+WK+KLV VP  VTVF V +TL+Q  T +  KW++IA 
Sbjct: 224 AMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIAD 283

Query: 253 KMDQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGL 312
           K+D DLF+ V        TK        ++TI +S+ + FLG   RL+ +M   F ELGL
Sbjct: 284 KLDDDLFIRVIISPASKTTK------PGNRTISMSYQAQFLGDSNRLLQVMQKSFPELGL 343

Query: 313 KRNDCTEMSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEAL 372
            + DCTEMSWI+SV++ A       PE L+     +    F AKSD+V  PI   GLE L
Sbjct: 344 TKKDCTEMSWIKSVMYIAGFPNSAAPEALL-AGKSLFKNHFKAKSDFVKEPIPVEGLEGL 403

Query: 373 WSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENAN-ETEKHLSW 432
           W + LEE+    I  PYGG M +ISESE PFPHR G ++ IQ+L+ W++     E+H+ W
Sbjct: 404 WERFLEEDSPLTIWNPYGGMMSRISESEIPFPHRNGTLFKIQWLSTWQDGKVSEERHMKW 463

Query: 433 MREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVK 492
           +RE+YSYM+ +VSK PR AY+NYRDLDLG N E  T   +A+ WG KY+KGNFERLV++K
Sbjct: 464 IREMYSYMEQYVSKNPRQAYVNYRDLDLGTN-EGET---DAREWGAKYYKGNFERLVKIK 523

Query: 493 TKVDPLNFFWNEQSIP 505
            + DP NFF +EQS+P
Sbjct: 524 GEFDPDNFFRHEQSVP 528

BLAST of Moc03g25060 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 510.4 bits (1313), Expect = 2.6e-144
Identity = 257/497 (51.71%), Postives = 344/497 (69.22%), Query Frame = 0

Query: 16  ISEVVFTNE--SASFSSLLRLSIRNLRFLTNSSPKPQFLVTPFRASHVQAAVVCAKKKSL 75
           + +  FT E  ++ F+ +L  + +N R+LT + PKP F+  P   SHVQA+V+C+KK  +
Sbjct: 51  LDKTFFTPERNASIFTEVLESTAQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEI 110

Query: 76  QVRVRSGGHDYEGLSYVSS-QTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIA 135
             RVRSGGHDYEG+SYVS  + PF++IDL  L  I++DI+  +A VE GAT+GE+YYRIA
Sbjct: 111 HFRVRSGGHDYEGVSYVSQIEKPFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIA 170

Query: 136 EKSPIHGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRIRDRNSMG 195
           EKS  HGFPAG  P++G+GGH++GG +G+L RKYGLAADNV+DAKIVD NG++ DR SMG
Sbjct: 171 EKSKFHGFPAGVYPSLGIGGHITGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMG 230

Query: 196 EDLFWAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGAT-HLFQKWQKIAHKM 255
           EDLFWAIRGG G SFG+ILSWK+KLV VP  +TVF V +T +Q  +  +  KWQ+IA  +
Sbjct: 231 EDLFWAIRGGSGGSFGIILSWKIKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNL 290

Query: 256 DQDLFLHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKR 315
             +LFL V   V  +K         ++KT+ +++   FLG    L+ +M   F ELGL +
Sbjct: 291 VDELFLRVFFTVSGNK---------ANKTVTMAYIGQFLGEKGTLMEVMKKDFPELGLTQ 350

Query: 316 NDCTEMSWIQSVLFFADLSTETPP--EILMERSPPISDTFFTAKSDYVTSPISQTGLEAL 375
            DC EMSWI S+++ +   T  PP  EIL++   PI   +F  KSD+   PI   GLE +
Sbjct: 351 KDCIEMSWIDSIIYNSGFPTNPPPPIEILLQAKSPIGKVYFKGKSDFAKKPIPVLGLEGM 410

Query: 376 WSKLLEEEKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAWENANE-TEKHLSW 435
           + KLLEE+ + +I TPYGGKMD+I ESE PFPHR G  + IQY  +W ++ +   +   W
Sbjct: 411 FKKLLEEDAALVIWTPYGGKMDKIPESEIPFPHRNGTNFMIQYYRSWSDSEKRPNRRTKW 470

Query: 436 MREVYSYMQPFVSKYPRGAYLNYRDLDLGRNYERNTS-YDEAKVWGLKYFKGNFERLVRV 495
           +RE+Y YM P+VS  PR AY+NYRDLDLG+N + + S + EAK+WG  YFK NF RLVR+
Sbjct: 471 IRELYGYMTPYVSSNPRQAYVNYRDLDLGQNKDNSKSNFIEAKIWGANYFKDNFNRLVRI 530

Query: 496 KTKVDPLNFFWNEQSIP 505
           K+KVDP NFF +EQSIP
Sbjct: 531 KSKVDPDNFFRHEQSIP 538

BLAST of Moc03g25060 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 509.6 bits (1311), Expect = 4.5e-144
Identity = 254/490 (51.84%), Postives = 343/490 (70.00%), Query Frame = 0

Query: 20  VFTNESASFSSLLRLSIRNLRFLT--NSSPKPQFLVTPFRASHVQAAVVCAKKKSLQVRV 79
           V+T+ S S  S      +N RFLT   +S KP  +VTP   + +Q +++C++K  ++VR 
Sbjct: 59  VYTDFSQSLIS------QNYRFLTLNFTSQKPILIVTPRTDTEIQRSLLCSRKLGVKVRT 118

Query: 80  RSGGHDYEGLSYVSSQTPFIVIDLINLSSIHIDIETETASVETGATLGEVYYRIAEKSPI 139
           +SGGHDYEGLSY+S  +PFI++DL+N+ SI I++  ETA V  GAT+GE+YY+IA+ S I
Sbjct: 119 KSGGHDYEGLSYLSLHSPFIILDLVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKI 178

Query: 140 HGFPAGSCPTVGVGGHVSGGGFGTLFRKYGLAADNVIDAKIVDFNGRI-RDRNSMGEDLF 199
           HGFPAG+CP+VGVGGH SGGGFG + RK+GLAADNV+DA+ VD NGRI   R  MGEDLF
Sbjct: 179 HGFPAGTCPSVGVGGHFSGGGFGAMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLF 238

Query: 200 WAIRGGGGASFGVILSWKLKLVSVPPDVTVFNVHRTLDQGATHLFQKWQKIAHKMDQDLF 259
           WAIRGGG ASFGV+LSWK+KLV VP  VT F  +  L Q  T +  +WQ+IA ++D +LF
Sbjct: 239 WAIRGGGAASFGVVLSWKVKLVRVPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLF 298

Query: 260 LHVTTKVVDHKTKLSSSKEASSKTIKLSFTSLFLGPIERLIPLMNTHFHELGLKRNDCTE 319
           + V   +             S  +++ +F + +LG I++LIPLMN  F ELGL   DC+E
Sbjct: 299 IRVIVSI-------------SGGSVQTTFQANYLGGIDKLIPLMNQKFPELGLTFQDCSE 358

Query: 320 MSWIQSVLFFADLSTETPPEILMERSPPISDTFFTAKSDYVTSPISQTGLEALWSKLLEE 379
           M+WI S+++F +     P E L++R    +D +F AKSD+V +PI + GLE +W++  E 
Sbjct: 359 MTWIDSIMYF-NWKKGQPLETLLDRGQRYNDLYFKAKSDFVKNPIPEIGLEGIWTRFHEV 418

Query: 380 EKSELILTPYGGKMDQISESETPFPHRRGNIYGIQYLAAW--ENANETEKHLSWMREVYS 439
           E   +I+ P GGKM +I E+ETPFPHRRGN+Y IQY+  W  ++    EKH++WMR +Y 
Sbjct: 419 ESPIMIMEPLGGKMYEIGETETPFPHRRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYR 478

Query: 440 YMQPFVSKYPRGAYLNYRDLDLGRNYERNTSYDEAKVWGLKYFKGNFERLVRVKTKVDPL 499
           YM+ +VS  PRGAYLNYRDLDLG N   NTS+++AK+WG +YF  NF+RL  VK K+DP 
Sbjct: 479 YMRVYVSASPRGAYLNYRDLDLGMNRGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPT 528

Query: 500 NFFWNEQSIP 505
           NFF NEQS+P
Sbjct: 539 NFFRNEQSVP 528