Moc03g24950 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g24950 vs. NCBI nr
Match: XP_022152545.1 (berberine bridge enzyme-like 13 [Momordica charantia])

HSP 1 Score: 835.1 bits (2156), Expect = 2.6e-238
Identity = 405/416 (97.36%), Postives = 407/416 (97.84%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG
Sbjct: 129 MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 188

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           MMLRKFGLAA+NVIDAYFVDVNGKLHNRKS GEDVFWAIRGGGGASF IIVAWKIKLVQ 
Sbjct: 189 MMLRKFGLAABNVIDAYFVDVNGKLHNRKSXGEDVFWAIRGGGGASFXIIVAWKIKLVQX 248

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF 180
           PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF
Sbjct: 249 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF 308

Query: 181 DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAF 240
           DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQ LDVLLDRNVAF
Sbjct: 309 DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQXLDVLLDRNVAF 368

Query: 241 EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA 300
           EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA
Sbjct: 369 EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA 428

Query: 301 GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN 360
           GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN
Sbjct: 429 GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN 488

Query: 361 GYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGHE 417
           GYTSYEKASIWGLRYFK NFKRLVSIKTKVDP NFF +EQSIPSLL WWT KEGHE
Sbjct: 489 GYTSYEKASIWGLRYFKGNFKRLVSIKTKVDPYNFFRHEQSIPSLLPWWT-KEGHE 543

BLAST of Moc03g24950 vs. NCBI nr
Match: XP_022152423.1 (berberine bridge enzyme-like 15 [Momordica charantia])

HSP 1 Score: 686.4 bits (1770), Expect = 1.5e-193
Identity = 331/416 (79.57%), Postives = 374/416 (89.90%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           + N+RSIDV+VEKNVAWVQSGATIGELYYRIA+KSKTLGFPAGLC TVGVGGHFSGGGYG
Sbjct: 128 LTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYG 187

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLAADNVIDAYF+DVNG L +RKSMGEDVFWAIRGGGGASF II+AWKIKL+ V
Sbjct: 188 VMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPV 247

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGM-NSNNHQNGKRTRETS 180
           PPVVTVF ITRNLEQNATNL+H+WQYVA+KQDD+LLI++ L GM NS+N+QN K+T E S
Sbjct: 248 PPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNXKKTLEAS 307

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F+SLFLGQ +ELLPIMQ TFPELGLTKEDCIEMSWIE+VL+FAGF+ GQPL+VLLDR++A
Sbjct: 308 FNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQ-GQPLNVLLDRSLA 367

Query: 241 FEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHR 300
           ++   K K+DYVNEPIP  G++GIWELFNEEEV+FAQ+  IPYGGKM+EI E EIPFPHR
Sbjct: 368 WKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEXEIPFPHR 427

Query: 301 AGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNK 360
            GNLYKIHY+V+   E EDT Q+ I+WIRKL SYMTPFVSKNPRAAYINYRDLDIG NNK
Sbjct: 428 VGNLYKIHYAVL-LGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTNNK 487

Query: 361 NGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGH 416
           +G TSY+KASIWG++YFKSNFKRLVS+KTKVDP NFF NEQSIPSL  WW KK GH
Sbjct: 488 DGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWW-KKRGH 540

BLAST of Moc03g24950 vs. NCBI nr
Match: XP_022954564.1 (berberine bridge enzyme-like 18 [Cucurbita moschata])

HSP 1 Score: 671.8 bits (1732), Expect = 3.9e-189
Identity = 318/417 (76.26%), Postives = 366/417 (87.77%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           +IN+RSI+V+ E NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGGHFSGGGYG
Sbjct: 127 LINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHFSGGGYG 186

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VAWKIKL+QV
Sbjct: 187 LMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWKIKLLQV 246

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG-KRTRETS 180
           PP+VTVF +TRNLEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q+G +RT E +
Sbjct: 247 PPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQRTLEAA 306

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+VLLDR   
Sbjct: 307 FNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVLLDRTPL 366

Query: 241 FEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPH 300
           F     KAK+DYVNEPIP   L+G WELFNEEE E A++   PYGG M+EI ESE PFPH
Sbjct: 367 FPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISESETPFPH 426

Query: 301 RAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINN 360
           RAGNLYKI + V W EE ED  ++HINWIRKL S++ P+VSKNPRAAYINYRDLDIG+NN
Sbjct: 427 RAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDLDIGMNN 486

Query: 361 KNGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGH 416
           KNG TSYE+AS+WG++YFK NFKRLVSIKTKVDP NFFMNEQSIPS+L  W KK GH
Sbjct: 487 KNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSW-KKRGH 542

BLAST of Moc03g24950 vs. NCBI nr
Match: KAG6573420.1 (Berberine bridge enzyme-like 18, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 669.8 bits (1727), Expect = 1.5e-188
Identity = 315/417 (75.54%), Postives = 366/417 (87.77%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           +IN+RSI+++ + NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGGHFSGGGYG
Sbjct: 127 LINLRSINIDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 186

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VAWKIKL+QV
Sbjct: 187 LMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWKIKLLQV 246

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG-KRTRETS 180
           PP+VTVF +TRNLEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q+G +RT E +
Sbjct: 247 PPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGINSTNPQHGSQRTLEAA 306

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+VLLDR   
Sbjct: 307 FNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVLLDRTPL 366

Query: 241 FEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPH 300
           F     KAK+DYVNEPIP   L+G WELFNEEE E A++   PYGG M+EI ESE PFPH
Sbjct: 367 FPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISESETPFPH 426

Query: 301 RAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINN 360
           RAGNLYKI + V W EE ED  ++HINWIRKL S++ P+VSKNPRAAYINYRDLD+G+NN
Sbjct: 427 RAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDLDVGMNN 486

Query: 361 KNGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGH 416
           KNG TSYE+AS+WG++YFK NFKRLVSIKTKVDP NFFMNEQSIPS+L  W KK GH
Sbjct: 487 KNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSW-KKRGH 542

BLAST of Moc03g24950 vs. NCBI nr
Match: XP_022152425.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 669.5 bits (1726), Expect = 1.9e-188
Identity = 321/416 (77.16%), Postives = 363/416 (87.26%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           + N+RSIDV+VE+ VAWVQSGATIGELYYRIA+KSKTLGFPAGLCPTVG+GGHFSGGGYG
Sbjct: 127 LTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYG 186

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLAADNVIDAYF+DVNGKLH+RKSMGEDVFWAIRGGGGASF I++AWKIKL+QV
Sbjct: 187 VMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWKIKLLQV 246

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF 180
           P +VTVF ITRNLEQNATNL++RWQYVANKQDD+L+IKI L G NSNNH+NGKRT E  F
Sbjct: 247 PXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKRTIEAFF 306

Query: 181 DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAF 240
           +SLFLG+ E+LLPIMQ TFPELGL KEDCIEMSWIESVL+FA F  GQPL+VLLDR   F
Sbjct: 307 NSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNVLLDRRSTF 366

Query: 241 EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA 300
           + + K K+DYVNEP+P  GL+GIWELFNE+E +  Q+F IPYGGKMNEI ESEIPFPHRA
Sbjct: 367 KGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESEIPFPHRA 426

Query: 301 GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN 360
           GNLY+IHYS+    ++E   Q++  WIRK  SYM PFVSKNPRAAYINYRDLDIG NN+N
Sbjct: 427 GNLYQIHYSIYCGGDEE---QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIGTNNEN 486

Query: 361 GYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGHE 417
           G TSY+KASIWG++YFK NFKRLVS+K KVDP NFF NEQSIPS   WW KK GHE
Sbjct: 487 GNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS--RWW-KKGGHE 536

BLAST of Moc03g24950 vs. ExPASy Swiss-Prot
Match: Q93ZA3 (Berberine bridge enzyme-like 13 OS=Arabidopsis thaliana OX=3702 GN=At1g30760 PE=1 SV=1)

HSP 1 Score: 460.7 bits (1184), Expect = 1.8e-128
Identity = 219/403 (54.34%), Postives = 291/403 (72.21%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           +R I V++E N AWV +GA+IGE+YYRI +KSK  GFPAGLC ++G+GGH  GG YG M+
Sbjct: 140 LRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMM 199

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RKFGL ADNV+DA  VD +GK+ NR +MGEDVFWAIRGGGG SF +I+AWKIKLV VP +
Sbjct: 200 RKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEI 259

Query: 124 VTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSL 183
           VTVF +TR LEQ+ T L+++WQ VA+K D+ L I++ ++   S   ++ +RT  TS+   
Sbjct: 260 VTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQ-PTSKTPKSKERTISTSYQGQ 319

Query: 184 FLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEAN 243
           FLG A  LL +MQ +FP+LGLTK+DC+E SWI+SV++ AGF    P + LLD    F+  
Sbjct: 320 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNY 379

Query: 244 GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNL 303
            KAK+DYV EPIP+ GL+G+WE   EE+       + PYGG M +IPE+E PFPHR+G L
Sbjct: 380 FKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETPFPHRSGTL 439

Query: 304 YKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNGYT 363
           +KI +  +W ++ + +  KH+ W+R++ SYM  +VSK+PR+AY+NYRDLD+G+N K    
Sbjct: 440 FKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKG--- 499

Query: 364 SYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLL 407
               A  WG RYFK NF+RLV IK K DP NFF +EQSIP+ L
Sbjct: 500 --SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Moc03g24950 vs. ExPASy Swiss-Prot
Match: Q9FKV2 (Berberine bridge enzyme-like 23 OS=Arabidopsis thaliana OX=3702 GN=At5g44360 PE=2 SV=1)

HSP 1 Score: 452.6 bits (1163), Expect = 4.8e-126
Identity = 217/408 (53.19%), Postives = 290/408 (71.08%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           ++N+RSI++N+    AWV +GATIGELYY+IA+ SK  GFPAG CP+VGVGGHFSGGG+G
Sbjct: 136 LVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGGHFSGGGFG 195

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHN-RKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQ 120
            M+RK GLAADNV+DA FVD NG+++N R+ MGED+FWAIRGGG ASF ++++WK+KLV+
Sbjct: 196 AMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVLSWKVKLVR 255

Query: 121 VPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETS 180
           VP  VT F     L QN T +VHRWQ +A + DD L I++ +             + +T+
Sbjct: 256 VPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG--------SVQTT 315

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F + +LG  ++L+P+M   FPELGLT +DC EM+WI+S+++F  ++ GQPL+ LLDR   
Sbjct: 316 FQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF-NWKKGQPLETLLDRGQR 375

Query: 241 F-EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPH 300
           + +   KAK+D+V  PIP +GL+GIW  F+  EVE   +   P GGKM EI E+E PFPH
Sbjct: 376 YNDLYFKAKSDFVKNPIPEIGLEGIWTRFH--EVESPIMIMEPLGGKMYEIGETETPFPH 435

Query: 301 RAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINN 360
           R GNLY I Y V W  +D    +KH+ W+R L  YM  +VS +PR AY+NYRDLD+G+ N
Sbjct: 436 RRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYRDLDLGM-N 495

Query: 361 KNGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLL 407
           +   TS+E A +WG RYF SNFKRL  +K K+DP NFF NEQS+P L+
Sbjct: 496 RGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPLI 531

BLAST of Moc03g24950 vs. ExPASy Swiss-Prot
Match: Q9FKU8 (Berberine bridge enzyme-like 26 OS=Arabidopsis thaliana OX=3702 GN=At5g44400 PE=2 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 2.4e-125
Identity = 216/405 (53.33%), Postives = 291/405 (71.85%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           +R +++N++ N AWVQSGAT+GELYYRIA+KSK  GFPAGLC ++G+GGH +GG YG M+
Sbjct: 140 MRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMM 199

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RK+GL ADNV+DA  VD NGKL +R +MGED FWAIRGG G SF II+AWKIKLV VP  
Sbjct: 200 RKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKT 259

Query: 124 VTVFEITRNLEQNATN-LVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDS 183
           VTVF +T+ L+Q+  N ++ +WQ VA+K  ++L I++      +     G +T  TS+++
Sbjct: 260 VTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT----GGNKTVTTSYNA 319

Query: 184 LFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAF-E 243
           LFLG    L+ +M+ +FPELGLT +DCIEMSW+ES+ + +GF    P +VLL     F +
Sbjct: 320 LFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPK 379

Query: 244 ANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAG 303
            + KAK+D+V  PIP  GLQGI++   +E++    + + PYGG M +IPES+IPFPHR G
Sbjct: 380 VSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKG 439

Query: 304 NLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNG 363
            L+K+ Y   W + D+    +HINWIR L SYMTP+VS NPR AY+NYRDLD+G N K+ 
Sbjct: 440 VLFKVQYVTSWLDSDK-RPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDV 499

Query: 364 YTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLL 407
            T  ++A +WG  YFK+NF RL+ IK KVDP NFF +EQSIP ++
Sbjct: 500 KTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537

BLAST of Moc03g24950 vs. ExPASy Swiss-Prot
Match: Q9FKV0 (Berberine bridge enzyme-like 24 OS=Arabidopsis thaliana OX=3702 GN=At5g44380 PE=2 SV=1)

HSP 1 Score: 444.5 bits (1142), Expect = 1.3e-123
Identity = 213/404 (52.72%), Postives = 290/404 (71.78%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           ++ I+V++E N AWVQ GAT+GELYYRIA+KSK  GFPAGLC +VG+GG+ +GGGYG ++
Sbjct: 143 LKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLM 202

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RK+GLA DNV+D   VD NGKL +R +MGED+FWAIRGGGGASF I++AWKIKLV VP  
Sbjct: 203 RKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKT 262

Query: 124 VTVFEITRNLEQNA-TNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDS 183
           VTVF +T+ LEQ+A    + +WQ +++K  +++ I++ LR   +    +G +T   ++  
Sbjct: 263 VTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN----DGNKTVTMTYLG 322

Query: 184 LFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLD-RNVAFE 243
            FLG+   LL +M+  FPELGLT++DC EMSWIE+ L   GF  G P+++LL  ++   +
Sbjct: 323 QFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGK 382

Query: 244 ANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAG 303
              KA +D+V EPIP++GL+GI++   E    F  + + PYGG M++IPES IPFPHR G
Sbjct: 383 DYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGMMSKIPESAIPFPHRNG 442

Query: 304 NLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNG 363
            L+KI Y   W E D+ T+ + INWI+++ +YM P+VS NPR AY+NYRDLD G N  N 
Sbjct: 443 TLFKILYYANWLENDK-TSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNA 502

Query: 364 YTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSL 406
             ++ +A IWG +YFK NF RLV IKTKVDP NFF +EQSIP +
Sbjct: 503 KVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

BLAST of Moc03g24950 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 443.7 bits (1140), Expect = 2.3e-123
Identity = 211/401 (52.62%), Postives = 286/401 (71.32%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           +R +DV+++ N AW  +GATIGE+YYRI +KS+T GFPAGLC ++G+GGH  GG YG M+
Sbjct: 138 LRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMM 197

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RKFGL ADNV+DA  VD NG++ +R +MGEDVFWAIRGGGG SF +I+AWKIKLV VP  
Sbjct: 198 RKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPAT 257

Query: 124 VTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSL 183
           VTVF +T+ LEQ+ T ++++W+ +A+K DD L I++ +    S   + G RT   S+ + 
Sbjct: 258 VTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVII-SPASKTTKPGNRTISMSYQAQ 317

Query: 184 FLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEAN 243
           FLG +  LL +MQ +FPELGLTK+DC EMSWI+SV++ AGF      + LL     F+ +
Sbjct: 318 FLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLFKNH 377

Query: 244 GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNL 303
            KAK+D+V EPIP+ GL+G+WE F EE+       + PYGG M+ I ESEIPFPHR G L
Sbjct: 378 FKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEIPFPHRNGTL 437

Query: 304 YKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNGYT 363
           +KI +   W ++ + + ++H+ WIR++ SYM  +VSKNPR AY+NYRDLD+G N   G T
Sbjct: 438 FKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTN--EGET 497

Query: 364 SYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPS 405
               A  WG +Y+K NF+RLV IK + DP NFF +EQS+P+
Sbjct: 498 D---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Moc03g24950 vs. ExPASy TrEMBL
Match: A0A6J1DE84 (berberine bridge enzyme-like 13 OS=Momordica charantia OX=3673 GN=LOC111020245 PE=3 SV=1)

HSP 1 Score: 835.9 bits (2158), Expect = 7.5e-239
Identity = 406/416 (97.60%), Postives = 407/416 (97.84%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG
Sbjct: 129 MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 188

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           MMLRKFGLAADNVIDAYFVDVNGKLHNRKS GEDVFWAIRGGGGASF IIVAWKIKLVQ 
Sbjct: 189 MMLRKFGLAADNVIDAYFVDVNGKLHNRKSXGEDVFWAIRGGGGASFXIIVAWKIKLVQX 248

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF 180
           PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF
Sbjct: 249 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF 308

Query: 181 DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAF 240
           DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQ LDVLLDRNVAF
Sbjct: 309 DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQXLDVLLDRNVAF 368

Query: 241 EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA 300
           EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA
Sbjct: 369 EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA 428

Query: 301 GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN 360
           GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN
Sbjct: 429 GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN 488

Query: 361 GYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGHE 417
           GYTSYEKASIWGLRYFK NFKRLVSIKTKVDP NFF +EQSIPSLL WWT KEGHE
Sbjct: 489 GYTSYEKASIWGLRYFKGNFKRLVSIKTKVDPYNFFRHEQSIPSLLPWWT-KEGHE 543

BLAST of Moc03g24950 vs. ExPASy TrEMBL
Match: A0A6J1DET9 (berberine bridge enzyme-like 15 OS=Momordica charantia OX=3673 GN=LOC111020159 PE=3 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 7.4e-194
Identity = 331/416 (79.57%), Postives = 374/416 (89.90%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           + N+RSIDV+VEKNVAWVQSGATIGELYYRIA+KSKTLGFPAGLC TVGVGGHFSGGGYG
Sbjct: 128 LTNLRSIDVDVEKNVAWVQSGATIGELYYRIAEKSKTLGFPAGLCSTVGVGGHFSGGGYG 187

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLAADNVIDAYF+DVNG L +RKSMGEDVFWAIRGGGGASF II+AWKIKL+ V
Sbjct: 188 VMLRKFGLAADNVIDAYFIDVNGNLFDRKSMGEDVFWAIRGGGGASFGIILAWKIKLLPV 247

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGM-NSNNHQNGKRTRETS 180
           PPVVTVF ITRNLEQNATNL+H+WQYVA+KQDD+LLI++ L GM NS+N+QN K+T E S
Sbjct: 248 PPVVTVFTITRNLEQNATNLIHQWQYVASKQDDRLLIRVILNGMINSSNNQNXKKTLEAS 307

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F+SLFLGQ +ELLPIMQ TFPELGLTKEDCIEMSWIE+VL+FAGF+ GQPL+VLLDR++A
Sbjct: 308 FNSLFLGQVDELLPIMQKTFPELGLTKEDCIEMSWIEAVLYFAGFQ-GQPLNVLLDRSLA 367

Query: 241 FEANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHR 300
           ++   K K+DYVNEPIP  G++GIWELFNEEEV+FAQ+  IPYGGKM+EI E EIPFPHR
Sbjct: 368 WKGTFKGKSDYVNEPIPKAGIEGIWELFNEEEVQFAQMLLIPYGGKMSEISEXEIPFPHR 427

Query: 301 AGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNK 360
            GNLYKIHY+V+   E EDT Q+ I+WIRKL SYMTPFVSKNPRAAYINYRDLDIG NNK
Sbjct: 428 VGNLYKIHYAVL-LGEGEDTAQRQISWIRKLYSYMTPFVSKNPRAAYINYRDLDIGTNNK 487

Query: 361 NGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGH 416
           +G TSY+KASIWG++YFKSNFKRLVS+KTKVDP NFF NEQSIPSL  WW KK GH
Sbjct: 488 DGNTSYDKASIWGMKYFKSNFKRLVSVKTKVDPSNFFRNEQSIPSLSPWW-KKRGH 540

BLAST of Moc03g24950 vs. ExPASy TrEMBL
Match: A0A6J1GTC1 (berberine bridge enzyme-like 18 OS=Cucurbita moschata OX=3662 GN=LOC111456799 PE=3 SV=1)

HSP 1 Score: 671.8 bits (1732), Expect = 1.9e-189
Identity = 318/417 (76.26%), Postives = 366/417 (87.77%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           +IN+RSI+V+ E NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGGHFSGGGYG
Sbjct: 127 LINLRSINVDAENNVAWVQSGATIGELYYRIAKKSKTLGFPAGVCPTVGVGGHFSGGGYG 186

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VAWKIKL+QV
Sbjct: 187 LMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWKIKLLQV 246

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG-KRTRETS 180
           PP+VTVF +TRNLEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q+G +RT E +
Sbjct: 247 PPIVTVFTVTRNLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQHGSQRTLEAA 306

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+VLLDR   
Sbjct: 307 FNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVLLDRTPL 366

Query: 241 FEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPH 300
           F     KAK+DYVNEPIP   L+G WELFNEEE E A++   PYGG M+EI ESE PFPH
Sbjct: 367 FPKRFFKAKSDYVNEPIPKAALEGTWELFNEEEAEAAEMILSPYGGIMDEISESETPFPH 426

Query: 301 RAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINN 360
           RAGNLYKI + V W EE ED  ++HINWIRKL S++ P+VSKNPRAAYINYRDLDIG+NN
Sbjct: 427 RAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSFVGPYVSKNPRAAYINYRDLDIGMNN 486

Query: 361 KNGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGH 416
           KNG TSYE+AS+WG++YFK NFKRLVSIKTKVDP NFFMNEQSIPS+L  W KK GH
Sbjct: 487 KNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPSW-KKRGH 542

BLAST of Moc03g24950 vs. ExPASy TrEMBL
Match: A0A6J1DDW6 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020161 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 9.3e-189
Identity = 321/416 (77.16%), Postives = 363/416 (87.26%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           + N+RSIDV+VE+ VAWVQSGATIGELYYRIA+KSKTLGFPAGLCPTVG+GGHFSGGGYG
Sbjct: 127 LTNLRSIDVDVEQKVAWVQSGATIGELYYRIAEKSKTLGFPAGLCPTVGIGGHFSGGGYG 186

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLAADNVIDAYF+DVNGKLH+RKSMGEDVFWAIRGGGGASF I++AWKIKL+QV
Sbjct: 187 VMLRKFGLAADNVIDAYFIDVNGKLHDRKSMGEDVFWAIRGGGGASFGIVLAWKIKLLQV 246

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSF 180
           P +VTVF ITRNLEQNATNL++RWQYVANKQDD+L+IKI L G NSNNH+NGKRT E  F
Sbjct: 247 PXLVTVFTITRNLEQNATNLIYRWQYVANKQDDRLIIKIILMGTNSNNHRNGKRTIEAFF 306

Query: 181 DSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAF 240
           +SLFLG+ E+LLPIMQ TFPELGL KEDCIEMSWIESVL+FA F  GQPL+VLLDR   F
Sbjct: 307 NSLFLGKVEDLLPIMQKTFPELGLMKEDCIEMSWIESVLYFAEFPRGQPLNVLLDRRSTF 366

Query: 241 EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRA 300
           + + K K+DYVNEP+P  GL+GIWELFNE+E +  Q+F IPYGGKMNEI ESEIPFPHRA
Sbjct: 367 KGSFKGKSDYVNEPVPKAGLEGIWELFNEKEAKMVQMFLIPYGGKMNEISESEIPFPHRA 426

Query: 301 GNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKN 360
           GNLY+IHYS+    ++E   Q++  WIRK  SYM PFVSKNPRAAYINYRDLDIG NN+N
Sbjct: 427 GNLYQIHYSIYCGGDEE---QRNKIWIRKFYSYMAPFVSKNPRAAYINYRDLDIGTNNEN 486

Query: 361 GYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKKEGHE 417
           G TSY+KASIWG++YFK NFKRLVS+K KVDP NFF NEQSIPS   WW KK GHE
Sbjct: 487 GNTSYDKASIWGVKYFKDNFKRLVSVKIKVDPSNFFRNEQSIPS--RWW-KKGGHE 536

BLAST of Moc03g24950 vs. ExPASy TrEMBL
Match: A0A6J1K043 (berberine bridge enzyme-like 18 OS=Cucurbita maxima OX=3661 GN=LOC111490403 PE=3 SV=1)

HSP 1 Score: 666.8 bits (1719), Expect = 6.0e-188
Identity = 312/414 (75.36%), Postives = 360/414 (86.96%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           +IN+RSI+V+ + NVAWVQSGATIGELYYRIA+KSKTLGFPAG+CPTVGVGGHFSGGGYG
Sbjct: 127 LINLRSINVDAKNNVAWVQSGATIGELYYRIAEKSKTLGFPAGVCPTVGVGGHFSGGGYG 186

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQV 120
           +MLRKFGLA DNVIDAYF+DVNG LH+RKSMGEDVFWA+RGGGGASF I+VAWKIKL+QV
Sbjct: 187 LMLRKFGLAVDNVIDAYFIDVNGNLHDRKSMGEDVFWALRGGGGASFGIVVAWKIKLLQV 246

Query: 121 PPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNG-KRTRETS 180
           PP+VTVF + R LEQNATNL+HRWQY+A+KQDD+L I+I +RG+NS N Q G +RT E +
Sbjct: 247 PPIVTVFTVPRTLEQNATNLIHRWQYIASKQDDRLFIRIIIRGVNSTNPQQGSQRTLEAA 306

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F+S+FLG++EELLPIMQNTFPELGLTKEDCIEMSWIES+L+FAGF  GQPL+VLLDR   
Sbjct: 307 FNSMFLGKSEELLPIMQNTFPELGLTKEDCIEMSWIESILYFAGFPSGQPLNVLLDRTPL 366

Query: 241 FEAN-GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPH 300
           F     KAK+DYVNEPIP   L+G WELF EEE E A++   PYGG M+EI ESE PFPH
Sbjct: 367 FPKRFFKAKSDYVNEPIPKAALEGTWELFKEEEAEAAEMILSPYGGIMDEISESETPFPH 426

Query: 301 RAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINN 360
           RAGNLYKI + V W EE ED  ++HINWIRKL SY+ P+VS NPRAAYINYRDLDIG+NN
Sbjct: 427 RAGNLYKIQHLVYWEEEGEDIAERHINWIRKLYSYVAPYVSNNPRAAYINYRDLDIGMNN 486

Query: 361 KNGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLLSWWTKK 413
           KNG TSYE+AS+WG++YFK NFKRLVSIKTKVDP NFFMNEQSIPS+L WW K+
Sbjct: 487 KNGKTSYEEASVWGVKYFKGNFKRLVSIKTKVDPSNFFMNEQSIPSILPWWKKR 540

BLAST of Moc03g24950 vs. TAIR 10
Match: AT1G30760.1 (FAD-binding Berberine family protein )

HSP 1 Score: 460.7 bits (1184), Expect = 1.3e-129
Identity = 219/403 (54.34%), Postives = 291/403 (72.21%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           +R I V++E N AWV +GA+IGE+YYRI +KSK  GFPAGLC ++G+GGH  GG YG M+
Sbjct: 140 LRQISVDIESNSAWVHAGASIGEVYYRIQEKSKIHGFPAGLCTSLGIGGHIIGGAYGSMM 199

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RKFGL ADNV+DA  VD +GK+ NR +MGEDVFWAIRGGGG SF +I+AWKIKLV VP +
Sbjct: 200 RKFGLGADNVLDARIVDADGKILNRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPEI 259

Query: 124 VTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSL 183
           VTVF +TR LEQ+ T L+++WQ VA+K D+ L I++ ++   S   ++ +RT  TS+   
Sbjct: 260 VTVFTVTRTLEQDGTKLLYKWQQVADKLDEDLFIRVIIQ-PTSKTPKSKERTISTSYQGQ 319

Query: 184 FLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEAN 243
           FLG A  LL +MQ +FP+LGLTK+DC+E SWI+SV++ AGF    P + LLD    F+  
Sbjct: 320 FLGDANRLLQVMQRSFPQLGLTKKDCLETSWIKSVMYIAGFPSTAPSEALLDGKSLFKNY 379

Query: 244 GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNL 303
            KAK+DYV EPIP+ GL+G+WE   EE+       + PYGG M +IPE+E PFPHR+G L
Sbjct: 380 FKAKSDYVEEPIPVEGLEGLWEKLLEEDSPLT--IWNPYGGMMAKIPETETPFPHRSGTL 439

Query: 304 YKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNGYT 363
           +KI +  +W ++ + +  KH+ W+R++ SYM  +VSK+PR+AY+NYRDLD+G+N K    
Sbjct: 440 FKIQWLTLW-QDGKTSEAKHMGWMREMYSYMEQYVSKSPRSAYVNYRDLDLGMNGKG--- 499

Query: 364 SYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLL 407
               A  WG RYFK NF+RLV IK K DP NFF +EQSIP+ L
Sbjct: 500 --SDAREWGNRYFKGNFERLVEIKAKFDPENFFRHEQSIPTEL 533

BLAST of Moc03g24950 vs. TAIR 10
Match: AT5G44360.1 (FAD-binding Berberine family protein )

HSP 1 Score: 452.6 bits (1163), Expect = 3.4e-127
Identity = 217/408 (53.19%), Postives = 290/408 (71.08%), Query Frame = 0

Query: 1   MINIRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYG 60
           ++N+RSI++N+    AWV +GATIGELYY+IA+ SK  GFPAG CP+VGVGGHFSGGG+G
Sbjct: 136 LVNVRSIEINLADETAWVGAGATIGELYYKIAKSSKIHGFPAGTCPSVGVGGHFSGGGFG 195

Query: 61  MMLRKFGLAADNVIDAYFVDVNGKLHN-RKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQ 120
            M+RK GLAADNV+DA FVD NG+++N R+ MGED+FWAIRGGG ASF ++++WK+KLV+
Sbjct: 196 AMMRKHGLAADNVVDARFVDANGRIYNSRREMGEDLFWAIRGGGAASFGVVLSWKVKLVR 255

Query: 121 VPPVVTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETS 180
           VP  VT F     L QN T +VHRWQ +A + DD L I++ +             + +T+
Sbjct: 256 VPEKVTCFRRNLPLTQNMTKIVHRWQQIAAELDDNLFIRVIVSISGG--------SVQTT 315

Query: 181 FDSLFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVA 240
           F + +LG  ++L+P+M   FPELGLT +DC EM+WI+S+++F  ++ GQPL+ LLDR   
Sbjct: 316 FQANYLGGIDKLIPLMNQKFPELGLTFQDCSEMTWIDSIMYF-NWKKGQPLETLLDRGQR 375

Query: 241 F-EANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPH 300
           + +   KAK+D+V  PIP +GL+GIW  F+  EVE   +   P GGKM EI E+E PFPH
Sbjct: 376 YNDLYFKAKSDFVKNPIPEIGLEGIWTRFH--EVESPIMIMEPLGGKMYEIGETETPFPH 435

Query: 301 RAGNLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINN 360
           R GNLY I Y V W  +D    +KH+ W+R L  YM  +VS +PR AY+NYRDLD+G+ N
Sbjct: 436 RRGNLYNIQYMVKWRLKDIGVMEKHVTWMRLLYRYMRVYVSASPRGAYLNYRDLDLGM-N 495

Query: 361 KNGYTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLL 407
           +   TS+E A +WG RYF SNFKRL  +K K+DP NFF NEQS+P L+
Sbjct: 496 RGVNTSFEDAKLWGFRYFGSNFKRLAIVKGKIDPTNFFRNEQSVPPLI 531

BLAST of Moc03g24950 vs. TAIR 10
Match: AT5G44400.1 (FAD-binding Berberine family protein )

HSP 1 Score: 450.3 bits (1157), Expect = 1.7e-126
Identity = 216/405 (53.33%), Postives = 291/405 (71.85%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           +R +++N++ N AWVQSGAT+GELYYRIA+KSK  GFPAGLC ++G+GGH +GG YG M+
Sbjct: 140 MRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFPAGLCSSLGIGGHITGGAYGSMM 199

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RK+GL ADNV+DA  VD NGKL +R +MGED FWAIRGG G SF II+AWKIKLV VP  
Sbjct: 200 RKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRGGAGGSFGIILAWKIKLVPVPKT 259

Query: 124 VTVFEITRNLEQNATN-LVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDS 183
           VTVF +T+ L+Q+  N ++ +WQ VA+K  ++L I++      +     G +T  TS+++
Sbjct: 260 VTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVLFNVAGT----GGNKTVTTSYNA 319

Query: 184 LFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAF-E 243
           LFLG    L+ +M+ +FPELGLT +DCIEMSW+ES+ + +GF    P +VLL     F +
Sbjct: 320 LFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAYISGFPTHTPTNVLLQGKSPFPK 379

Query: 244 ANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAG 303
            + KAK+D+V  PIP  GLQGI++   +E++    + + PYGG M +IPES+IPFPHR G
Sbjct: 380 VSFKAKSDFVKTPIPESGLQGIFKKLLKEDIPL--MIWNPYGGMMAKIPESQIPFPHRKG 439

Query: 304 NLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNG 363
            L+K+ Y   W + D+    +HINWIR L SYMTP+VS NPR AY+NYRDLD+G N K+ 
Sbjct: 440 VLFKVQYVTSWLDSDK-RPSRHINWIRDLYSYMTPYVSSNPREAYVNYRDLDLGRNTKDV 499

Query: 364 YTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSLL 407
            T  ++A +WG  YFK+NF RL+ IK KVDP NFF +EQSIP ++
Sbjct: 500 KTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENFFRHEQSIPPMM 537

BLAST of Moc03g24950 vs. TAIR 10
Match: AT5G44380.1 (FAD-binding Berberine family protein )

HSP 1 Score: 444.5 bits (1142), Expect = 9.4e-125
Identity = 213/404 (52.72%), Postives = 290/404 (71.78%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           ++ I+V++E N AWVQ GAT+GELYYRIA+KSK  GFPAGLC +VG+GG+ +GGGYG ++
Sbjct: 143 LKQINVDIESNSAWVQPGATLGELYYRIAEKSKIHGFPAGLCTSVGIGGYMTGGGYGTLM 202

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RK+GLA DNV+D   VD NGKL +R +MGED+FWAIRGGGGASF I++AWKIKLV VP  
Sbjct: 203 RKYGLAGDNVLDVKMVDANGKLLDRAAMGEDLFWAIRGGGGASFGIVLAWKIKLVPVPKT 262

Query: 124 VTVFEITRNLEQNA-TNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDS 183
           VTVF +T+ LEQ+A    + +WQ +++K  +++ I++ LR   +    +G +T   ++  
Sbjct: 263 VTVFTVTKTLEQDARLKTISKWQQISSKIIEEIHIRVVLRAAGN----DGNKTVTMTYLG 322

Query: 184 LFLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLD-RNVAFE 243
            FLG+   LL +M+  FPELGLT++DC EMSWIE+ L   GF  G P+++LL  ++   +
Sbjct: 323 QFLGEKGTLLKVMEKAFPELGLTQKDCTEMSWIEAALFHGGFPTGSPIEILLQLKSPLGK 382

Query: 244 ANGKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAG 303
              KA +D+V EPIP++GL+GI++   E    F  + + PYGG M++IPES IPFPHR G
Sbjct: 383 DYFKATSDFVKEPIPVIGLKGIFKRLIEGNTTF--LNWTPYGGMMSKIPESAIPFPHRNG 442

Query: 304 NLYKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNG 363
            L+KI Y   W E D+ T+ + INWI+++ +YM P+VS NPR AY+NYRDLD G N  N 
Sbjct: 443 TLFKILYYANWLENDK-TSSRKINWIKEIYNYMAPYVSSNPRQAYVNYRDLDFGQNKNNA 502

Query: 364 YTSYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPSL 406
             ++ +A IWG +YFK NF RLV IKTKVDP NFF +EQSIP +
Sbjct: 503 KVNFIEAKIWGPKYFKGNFDRLVKIKTKVDPENFFRHEQSIPPM 539

BLAST of Moc03g24950 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 443.7 bits (1140), Expect = 1.6e-124
Identity = 211/401 (52.62%), Postives = 286/401 (71.32%), Query Frame = 0

Query: 4   IRSIDVNVEKNVAWVQSGATIGELYYRIAQKSKTLGFPAGLCPTVGVGGHFSGGGYGMML 63
           +R +DV+++ N AW  +GATIGE+YYRI +KS+T GFPAGLC ++G+GGH  GG YG M+
Sbjct: 138 LRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIGGHLVGGAYGSMM 197

Query: 64  RKFGLAADNVIDAYFVDVNGKLHNRKSMGEDVFWAIRGGGGASFDIIVAWKIKLVQVPPV 123
           RKFGL ADNV+DA  VD NG++ +R +MGEDVFWAIRGGGG SF +I+AWKIKLV VP  
Sbjct: 198 RKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVILAWKIKLVPVPAT 257

Query: 124 VTVFEITRNLEQNATNLVHRWQYVANKQDDKLLIKITLRGMNSNNHQNGKRTRETSFDSL 183
           VTVF +T+ LEQ+ T ++++W+ +A+K DD L I++ +    S   + G RT   S+ + 
Sbjct: 258 VTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVII-SPASKTTKPGNRTISMSYQAQ 317

Query: 184 FLGQAEELLPIMQNTFPELGLTKEDCIEMSWIESVLHFAGFRGGQPLDVLLDRNVAFEAN 243
           FLG +  LL +MQ +FPELGLTK+DC EMSWI+SV++ AGF      + LL     F+ +
Sbjct: 318 FLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIAGFPNSAAPEALLAGKSLFKNH 377

Query: 244 GKAKTDYVNEPIPIVGLQGIWELFNEEEVEFAQIFFIPYGGKMNEIPESEIPFPHRAGNL 303
            KAK+D+V EPIP+ GL+G+WE F EE+       + PYGG M+ I ESEIPFPHR G L
Sbjct: 378 FKAKSDFVKEPIPVEGLEGLWERFLEEDSPLT--IWNPYGGMMSRISESEIPFPHRNGTL 437

Query: 304 YKIHYSVVWTEEDEDTTQKHINWIRKLESYMTPFVSKNPRAAYINYRDLDIGINNKNGYT 363
           +KI +   W ++ + + ++H+ WIR++ SYM  +VSKNPR AY+NYRDLD+G N   G T
Sbjct: 438 FKIQWLSTW-QDGKVSEERHMKWIREMYSYMEQYVSKNPRQAYVNYRDLDLGTN--EGET 497

Query: 364 SYEKASIWGLRYFKSNFKRLVSIKTKVDPRNFFMNEQSIPS 405
               A  WG +Y+K NF+RLV IK + DP NFF +EQS+P+
Sbjct: 498 D---AREWGAKYYKGNFERLVKIKGEFDPDNFFRHEQSVPT 529