Homology
BLAST of Moc03g24700 vs. NCBI nr
Match:
XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])
HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0
Query: 3 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 62
MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS
Sbjct: 1 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 60
Query: 63 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 122
SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG
Sbjct: 61 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 120
Query: 123 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 182
LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT
Sbjct: 121 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 180
Query: 183 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 242
VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 240
Query: 243 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 302
GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT
Sbjct: 241 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 300
Query: 303 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 362
SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT
Sbjct: 301 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 360
Query: 363 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 422
ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM
Sbjct: 361 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 420
Query: 423 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 482
SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 480
Query: 483 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL
Sbjct: 481 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539
BLAST of Moc03g24700 vs. NCBI nr
Match:
XP_022142425.1 (berberine bridge enzyme-like 18 [Momordica charantia])
HSP 1 Score: 963.8 bits (2490), Expect = 6.4e-277
Identity = 469/541 (86.69%), Postives = 497/541 (91.87%), Query Frame = 0
Query: 1 MNMNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 60
MN Y SPLI L LAFIFLSSS W A +KHESFLQCLL+HS + NYSISKVIHTPI
Sbjct: 1 MNKYYSSPLIPLFLAFIFLSSSRFWTLAVADKHESFLQCLLNHSPD-GNYSISKVIHTPI 60
Query: 61 NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDF 120
NSSYSSVLDFSIRNLRFST ETPKPLLI+TPSHVSHIQAAV+CSK YGFQIRTRSGGHDF
Sbjct: 61 NSSYSSVLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDF 120
Query: 121 EGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 180
E LSYVAHL FIIVDLINLRSIS+D+K+N AWV SGATLGELYYSIAQKSRT AFPAG+C
Sbjct: 121 ERLSYVAHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVC 180
Query: 181 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 240
PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGG 240
Query: 241 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGK 300
SFGIVVAWKVKLVPVPATVTLC NRTLEEGAVK+THRWQYVANKLDENLFLGILWLGG
Sbjct: 241 SFGIVVAWKVKLVPVPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGN 300
Query: 301 ITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGF 360
+TSQ GGKTNPVATFFSLFLG+ADELL+ILNTKFPELDLAKKDCIETSWIESTVLMG+GF
Sbjct: 301 VTSQGGGKTNPVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGF 360
Query: 361 PTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGG 420
T++TLEALLSRTPLTN S+KIKSDYVKEP+SEA +QGI +RL++QDIE GN+IFVPYGG
Sbjct: 361 QTKVTLEALLSRTPLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFVPYGG 420
Query: 421 RMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRA 480
RMSQI ESETPFSHRAGYLYKIGYI W +Q ID EKRHL WIRELY YM PFVSKSPRA
Sbjct: 421 RMSQIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSKSPRA 480
Query: 481 AYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPA 540
YANYRDLDIGSN ++GKTSYKQAS WG KYFGNNFNRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 VYANYRDLDIGSNKRYGKTSYKQASTWGFKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 540
Query: 541 L 542
L
Sbjct: 541 L 540
BLAST of Moc03g24700 vs. NCBI nr
Match:
XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])
HSP 1 Score: 882.9 bits (2280), Expect = 1.4e-252
Identity = 427/541 (78.93%), Postives = 480/541 (88.72%), Query Frame = 0
Query: 3 MNYPSPLI--SLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 62
M Y SPLI S SL FIFLSSSSLWA+A +KHESFL CLLDHS + SNYSISKV++TP+
Sbjct: 1 MKYSSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPD-SNYSISKVVYTPL 60
Query: 63 NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDF 122
NSSYSSVLDFSIRNLRFST +TPKPL I+TP HVSH+QAAV+CS+++G QIRTRSGGHDF
Sbjct: 61 NSSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDF 120
Query: 123 EGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 182
EGLS V+HLPFII+DLINLRSI+ID +NTAWVQSGATLGELYYSIA+KSRTLAFPAG C
Sbjct: 121 EGLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGAC 180
Query: 183 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 242
PTVG+GGHFSGGGYGL+LRK+GLAADNVIDAYLVDA+GKF +RE MGEDLFWAIRGGGGG
Sbjct: 181 PTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGG 240
Query: 243 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGK 302
SFGIVVAWKVKLVPVPATVT C NRTLEEGA+K+ HRWQYVANKLDENLFLGIL +GG
Sbjct: 241 SFGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGN 300
Query: 303 ITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGF 362
T+Q G KTNP A+FFSLFLGKADE +ILNT FPEL LAK++C+E+SWIES L+ GF
Sbjct: 301 FTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIES-ALLSAGF 360
Query: 363 PTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGG 422
+LEALL+RTPLTN S KIKSDYVKEP+SEA ++GIW+RL S+D+ ++FVPYGG
Sbjct: 361 KIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 420
Query: 423 RMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRA 482
RMSQISESE PFSHRAG LYKIGYI W++Q +D EKRHL+WIRELY YMTPFVSKSPR+
Sbjct: 421 RMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRS 480
Query: 483 AYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPA 542
AY NYRDLDIGSNNK+GKTS+K+ASVWG KYFGNNFNRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 539
BLAST of Moc03g24700 vs. NCBI nr
Match:
XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])
HSP 1 Score: 850.5 bits (2196), Expect = 7.9e-243
Identity = 411/539 (76.25%), Postives = 471/539 (87.38%), Query Frame = 0
Query: 3 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 62
M Y SPLI LSL I +SSS LWASA K++ FLQCLL+HSL+ NY ISKV+++PINS
Sbjct: 1 MKYTSPLILLSLVLIVVSSSPLWASASEQKYQHFLQCLLEHSLD--NYPISKVVYSPINS 60
Query: 63 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 122
SYSSVLDFSIRNLRFS +TPKPLLI+TPSHVSHIQAAVICSKS+G QIRTRSGGHDFEG
Sbjct: 61 SYSSVLDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120
Query: 123 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 182
LSYVA+LPFIIVDLINLRS++ID++++TAWVQSGATLGELYY I +KSRTLAFPAG+CPT
Sbjct: 121 LSYVAYLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPT 180
Query: 183 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 242
VGVGGHFSGGGYG +LRK+GLAADNVIDAYLVDA+GK H+RESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSF 240
Query: 243 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 302
GIVVAWKVKLVPVPATVT CS +R LE A+K+ H+WQYVANKLDENLFLGIL GG IT
Sbjct: 241 GIVVAWKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNIT 300
Query: 303 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 362
+ + G +P ATFF LFLGK DEL++ L+T FPEL L K+DC+ETSWIEST++ G
Sbjct: 301 TTQEGIADPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAATGVQI 360
Query: 363 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 422
+LE LL+RTP T S+KIKSDY+KEP+ EA ++GI++RL +QDI +IFVPYGGRM
Sbjct: 361 VESLEPLLNRTPPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVPYGGRM 420
Query: 423 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 482
SQISESETPFSHRA LYKIGYI GW+EQ ++A+KRH+ WIRE+Y+YMTPFVSKSPR+AY
Sbjct: 421 SQISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHISWIREVYEYMTPFVSKSPRSAY 480
Query: 483 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
ANYRDLDIG+NNK+ KTS KQASVWGLKYFGNNF RLV+VKTKVDPYDFFRHEQSIPAL
Sbjct: 481 ANYRDLDIGANNKYRKTSVKQASVWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPAL 537
BLAST of Moc03g24700 vs. NCBI nr
Match:
XP_004151192.3 (berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical protein Csa_023212 [Cucumis sativus])
HSP 1 Score: 842.4 bits (2175), Expect = 2.1e-240
Identity = 403/539 (74.77%), Postives = 464/539 (86.09%), Query Frame = 0
Query: 3 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 62
M Y SPLI SL I +SSSSLW SA P+KH +FLQCL +HS + +Y ISKV+HTPINS
Sbjct: 1 MKYSSPLIPFSLILIVVSSSSLWVSASPHKHHNFLQCLSEHSSK--SYPISKVVHTPINS 60
Query: 63 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 122
SYSSVL+FSIRNLRFS ETPKPLLI+TPSHVSHIQAAVICSKS+G QIRTRSGGHD+EG
Sbjct: 61 SYSSVLNFSIRNLRFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDYEG 120
Query: 123 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 182
LSYVA PFII+DLINLRS+ ID++ NTAWV+SG TLGELYY I +KSRTLAFPAGICPT
Sbjct: 121 LSYVASHPFIILDLINLRSMKIDVESNTAWVESGTTLGELYYRIGEKSRTLAFPAGICPT 180
Query: 183 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 242
VGVGGHFSGGGYGL+LRK+GLAADNVIDAYLVDA+GK H+RESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSF 240
Query: 243 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 302
GIVVAWK+KLV VPATVT+C+ R L+ +K+ H+WQYVANKLDENLFLGI+ GG IT
Sbjct: 241 GIVVAWKIKLVSVPATVTMCNTYRNLKGDGIKLVHQWQYVANKLDENLFLGIILTGGNIT 300
Query: 303 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 362
+ + G TNP+ATFFSLFLGK +EL++ L+T FPEL L K+DCIETSWIEST++ G T
Sbjct: 301 TTQEGITNPIATFFSLFLGKVNELVATLSTTFPELGLIKQDCIETSWIESTLIASTGVQT 360
Query: 363 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 422
+LE LL+RTP + KIKSDY+KEP+S A ++GIW+RL +QDIE +IF+PYGGRM
Sbjct: 361 VESLEPLLNRTPSNLENEKIKSDYIKEPISIATIEGIWQRLKAQDIETSQLIFIPYGGRM 420
Query: 423 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 482
SQISESETPFSHR G LYKIGYI GW+EQ + A+K+H+ WIRE+Y+YMTPFVSKSPR+AY
Sbjct: 421 SQISESETPFSHRVGNLYKIGYILGWKEQSLKAKKKHISWIREIYEYMTPFVSKSPRSAY 480
Query: 483 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
ANYRDLDIG N K+GKTS KQAS+WGLKYFGNNF RLV+VKTKVDPYDFFRHEQSIP L
Sbjct: 481 ANYRDLDIGVNKKYGKTSVKQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537
BLAST of Moc03g24700 vs. ExPASy Swiss-Prot
Match:
Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)
HSP 1 Score: 540.8 bits (1392), Expect = 1.8e-152
Identity = 271/533 (50.84%), Postives = 364/533 (68.29%), Query Frame = 0
Query: 14 LAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVLDFSIR 73
+ F FLS + A N E+FL+C H +N + K+++T + Y S+L+ +I+
Sbjct: 14 IIFFFLSFHIQISIA--NPRENFLKCFSKHI--PNNVANPKLVYTQHDQLYMSILNSTIQ 73
Query: 74 NLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAHLPFII 133
NLRF + TPKPL+IVTPS+ SHIQA ++CSK G QIRTRSGGHD EG+SY++ +PF++
Sbjct: 74 NLRFISDTTPKPLVIVTPSNNSHIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVV 133
Query: 134 VDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGG 193
VDL N+ SI ID+ TAWV++GATLGE+YY I +K+ L+FP G CPTVGVGGHFSGGG
Sbjct: 134 VDLRNMHSIKIDVHSQTAWVEAGATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGG 193
Query: 194 YGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 253
YG L+R YGLAADN+IDA+LV+ GK +R+SMGEDLFWAIRGGGG +FGI+ AWK+KLV
Sbjct: 194 YGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLV 253
Query: 254 PVPATVTLCSANRTLE-EGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG-GKTNP 313
VP+ T+ S + +E G VK+ ++WQ +A K D++L L ++ IT G KT
Sbjct: 254 AVPSKSTIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTV 313
Query: 314 VATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG--VGFPT-ELTLEA 373
F S+F G D L+ ++N FPEL + K DC E SWI++T+ V F T E
Sbjct: 314 HGYFSSIFHGGVDSLVDLMNKSFPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEI 373
Query: 374 LLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISES 433
LL R+ T+ IK DYVK+P+ E + I ++L +D+ G + PYGG M +ISES
Sbjct: 374 LLDRSAGKKTAFSIKLDYVKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISES 433
Query: 434 ETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDL 493
PF HRAG +Y++ Y A WE+Q + ++H+ W+R +Y++ TP+VS++PR AY NYRDL
Sbjct: 434 AIPFPHRAGIMYELWYTASWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDL 493
Query: 494 DIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
D+G N +Y QA +WG KYFG NFNRLV VKTKVDP +FFR+EQSIP L
Sbjct: 494 DLGKTNHASPNNYTQARIWGEKYFGKNFNRLVKVKTKVDPNNFFRNEQSIPPL 540
BLAST of Moc03g24700 vs. ExPASy Swiss-Prot
Match:
A6P6V9 (Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1)
HSP 1 Score: 533.5 bits (1373), Expect = 2.8e-150
Identity = 270/530 (50.94%), Postives = 363/530 (68.49%), Query Frame = 0
Query: 17 IFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVLDFSIRNLR 76
IF S ++ N E+FL+C + +N + K+++T N Y SVL+ +I NLR
Sbjct: 15 IFFFFSFNIQTSIANPRENFLKCFSQYI--PNNATNLKLVYTQNNPLYMSVLNSTIHNLR 74
Query: 77 FSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAHLPFIIVDL 136
F++ TPKPL+IVTPSHVSHIQ ++CSK G QIRTRSGGHD EG+SY++ +PF+IVDL
Sbjct: 75 FTSDTTPKPLVIVTPSHVSHIQGTILCSKKVGLQIRTRSGGHDSEGMSYISQVPFVIVDL 134
Query: 137 INLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGGYGL 196
N+RSI ID+ TAWV++GATLGE+YY + +K+ L+ AG CPTV GGHF GGGYG
Sbjct: 135 RNMRSIKIDVHSQTAWVEAGATLGEVYYWVNEKNENLSLAAGYCPTVCAGGHFGGGGYGP 194
Query: 197 LLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVP 256
L+R YGLAADN+IDA+LV+ GK +R+SMGEDLFWA+RGGG SFGI+VAWK++LV VP
Sbjct: 195 LMRNYGLAADNIIDAHLVNVHGKVLDRKSMGEDLFWALRGGGAESFGIIVAWKIRLVAVP 254
Query: 257 ATVTLCSANRTLE-EGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVATF 316
+ T+ S + +E VK+ ++WQ +A K D++L L ++ IT +G + T+
Sbjct: 255 KS-TMFSVKKIMEIHELVKLVNKWQNIAYKYDKDLLLMTHFITRNITDNQGKNKTAIHTY 314
Query: 317 F-SLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG--VGFPTE-LTLEALLS 376
F S+FLG D L+ ++N FPEL + K DC + SWI++ + V + T+ E LL
Sbjct: 315 FSSVFLGGVDSLVDLMNKSFPELGIKKTDCRQLSWIDTIIFYSGVVNYDTDNFNKEILLD 374
Query: 377 RTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETP 436
R+ N + KIK DYVK+P+ E+V I ++L +DI G PYGG M +ISES P
Sbjct: 375 RSAGQNGAFKIKLDYVKKPIPESVFVQILEKLYEEDIGAGMYALYPYGGIMDEISESAIP 434
Query: 437 FSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDLDIG 496
F HRAG LY++ YI WE+Q + ++HL WIR +Y++MTP+VSK+PR AY NYRDLDIG
Sbjct: 435 FPHRAGILYELWYICSWEKQ--EDNEKHLNWIRNIYNFMTPYVSKNPRLAYLNYRDLDIG 494
Query: 497 SNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
N+ +Y QA +WG KYFG NF+RLV VKT VDP +FFR+EQSIP L
Sbjct: 495 INDPKNPNNYTQARIWGEKYFGKNFDRLVKVKTLVDPNNFFRNEQSIPPL 539
BLAST of Moc03g24700 vs. ExPASy Swiss-Prot
Match:
Q9SVG5 (Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=3 SV=1)
HSP 1 Score: 531.9 bits (1369), Expect = 8.1e-150
Identity = 269/536 (50.19%), Postives = 369/536 (68.84%), Query Frame = 0
Query: 13 SLAFIFLSSSSLW------ASAFPNKHESFLQCLLDHSLEYSNYSI-SKVIHTPINSSYS 72
S IF ++S+L SA + SFLQCL SL+ ++ +I SKVIHTP ++S+S
Sbjct: 8 SSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL---SLQLNDSNIVSKVIHTPNDTSFS 67
Query: 73 SVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSY 132
SVL SI+N RFS + PKP+LI+TP S +Q+AV C++ +G IRTRSGGHD+EGLSY
Sbjct: 68 SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 127
Query: 133 VAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGV 192
V H PF+I+DL NLRSI++D+ + + WVQ+GAT+GELYY I +K+RTLAFPAG+CPTVGV
Sbjct: 128 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 187
Query: 193 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIV 252
GGHFSGGGYG LLRK+GLAAD+VIDA +VDA G+ R MGED FWAIRGGGG SF +V
Sbjct: 188 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 247
Query: 253 VAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQE 312
++WK+ L+ VP+TVT+ + + E+ A+KI HRWQ+VA+K+ ++LF+ ++ +
Sbjct: 248 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVM--------LQ 307
Query: 313 GGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELT 372
K A+F L+LG LL ++N +FPEL L + DC E SWIES + EL
Sbjct: 308 RYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWF-----AELG 367
Query: 373 LEAL--LSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 432
E + L++ + + K KSD+V+EPM + + +W+RL + E +IF P+GG+MS
Sbjct: 368 EEPINVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMS 427
Query: 433 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 492
+I++ ETPF HR G +Y+I Y+ W D +++++RW+ +YD M+ FV+KSPR AY
Sbjct: 428 EIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYI 487
Query: 493 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 540
N RDLD+G ++ Y++ WG+KYF NNF RLV VKT VDP DFF EQSIP
Sbjct: 488 NLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524
BLAST of Moc03g24700 vs. ExPASy Swiss-Prot
Match:
Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)
HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 268/529 (50.66%), Postives = 368/529 (69.57%), Query Frame = 0
Query: 17 IFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSIS-KVIHTPINSSYSSVLDFSIRNL 76
+F + S SA + HE FL+CL S ++ ++ KVIHT +SS+ S+LD SI+N
Sbjct: 12 LFSLNISPLVSAHGSNHEDFLKCL---SYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNP 71
Query: 77 RFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAH-LPFIIV 136
RFS ETPKP+ I+TP S +Q + C++ +G +RTRS GH +EGLSY+A+ PF ++
Sbjct: 72 RFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVI 131
Query: 137 DLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGGY 196
DL NLRSIS+D+ + T WVQ+GAT GELYY I + +++LAFPAGI PTVGVGG FSGGGY
Sbjct: 132 DLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGY 191
Query: 197 GLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 256
G LLRKYGLAADN+IDA +VDASG+ +R++MGED FWAIRGGGG SFG++++WKVKLV
Sbjct: 192 GTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVD 251
Query: 257 VPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVAT 316
VP+T+T+ +T ++ AV+I +WQY A+K+ ++LF+ + T + K A
Sbjct: 252 VPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHAL 311
Query: 317 FFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLSRTP 376
F L++G + LL+++ KFPEL L K+ C E SWIES VL FP +L L +R
Sbjct: 312 FTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIES-VLWFADFPKGESLGVLTNR-E 371
Query: 377 LTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETPFSH 436
T+ S K K D+V+EP+ EA +Q IW+RL++ + G II P+GG+MS+++E ETPF H
Sbjct: 372 RTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPH 431
Query: 437 RAGYLYKIGYIAGWEEQGIDAEK----RHLRWIRELYDYMTPFVSKSPRAAYANYRDLDI 496
R G LY+I Y+A W E+ D K ++L+W+ +Y++MTP+VSKSPR AY N++D+D+
Sbjct: 432 RGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDL 491
Query: 497 GSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 540
G KT Y++ WG+KYF NNF RLV VKT+VDP DFF EQSIP
Sbjct: 492 GMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527
BLAST of Moc03g24700 vs. ExPASy Swiss-Prot
Match:
Q33DQ2 (Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1)
HSP 1 Score: 530.4 bits (1365), Expect = 2.4e-149
Identity = 268/533 (50.28%), Postives = 363/533 (68.11%), Query Frame = 0
Query: 14 LAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSSVLDFSIR 73
+ F FLS + + A N E+FL+C ++ +N + K I+T + Y SVL+ +I+
Sbjct: 14 IIFFFLSFNIQISIA--NPQENFLKCFSEYI--PNNPANPKFIYTQHDQLYMSVLNSTIQ 73
Query: 74 NLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAHLPFII 133
NLRF++ TPKPL+IVTPS+VSHIQA+++CSK G QIRTRSGGHD EGLSY++ +PF I
Sbjct: 74 NLRFTSDTTPKPLVIVTPSNVSHIQASILCSKKVGLQIRTRSGGHDAEGLSYISQVPFAI 133
Query: 134 VDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGG 193
VDL N+ ++ +DI TAWV++GATLGE+YY I + + +FP G CPTVGVGGHFSGGG
Sbjct: 134 VDLRNMHTVKVDIHSQTAWVEAGATLGEVYYWINEMNENFSFPGGYCPTVGVGGHFSGGG 193
Query: 194 YGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLV 253
YG L+R YGLAADN+IDA+LV+ GK +R+SMGEDLFWAIRGGGG +FGI+ AWK+KLV
Sbjct: 194 YGALMRNYGLAADNIIDAHLVNVDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLV 253
Query: 254 PVPATVTLCSANRTLE-EGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG-GKTNP 313
VP+ T+ S + +E G VK+ ++WQ +A K D++L L + IT G KT
Sbjct: 254 VVPSKATIFSVKKNMEIHGLVKLFNKWQNIAYKYDKDLMLTTHFRTRNITDNHGKNKTTV 313
Query: 314 VATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMG--VGFPT-ELTLEA 373
F S+FLG D L+ ++N FPEL + K DC E SWI++T+ V + T E
Sbjct: 314 HGYFSSIFLGGVDSLVDLMNKSFPELGIKKTDCKELSWIDTTIFYSGVVNYNTANFKKEI 373
Query: 374 LLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISES 433
LL R+ T+ IK DYVK+ + E + I ++L +++ G + PYGG M +ISES
Sbjct: 374 LLDRSAGKKTAFSIKLDYVKKLIPETAMVKILEKLYEEEVGVGMYVLYPYGGIMDEISES 433
Query: 434 ETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYANYRDL 493
PF HRAG +Y++ Y A WE+Q + ++H+ W+R +Y++ TP+VS++PR AY NYRDL
Sbjct: 434 AIPFPHRAGIMYELWYTATWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDL 493
Query: 494 DIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
D+G N +Y QA +WG KYFG NFNRLV VKTK DP +FFR+EQSIP L
Sbjct: 494 DLGKTNPESPNNYTQARIWGEKYFGKNFNRLVKVKTKADPNNFFRNEQSIPPL 540
BLAST of Moc03g24700 vs. ExPASy TrEMBL
Match:
A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)
HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0
Query: 3 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 62
MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS
Sbjct: 1 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 60
Query: 63 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 122
SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG
Sbjct: 61 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 120
Query: 123 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 182
LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT
Sbjct: 121 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 180
Query: 183 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 242
VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 240
Query: 243 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 302
GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT
Sbjct: 241 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 300
Query: 303 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 362
SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT
Sbjct: 301 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 360
Query: 363 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 422
ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM
Sbjct: 361 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 420
Query: 423 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 482
SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY
Sbjct: 421 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 480
Query: 483 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL
Sbjct: 481 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 539
BLAST of Moc03g24700 vs. ExPASy TrEMBL
Match:
A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)
HSP 1 Score: 963.8 bits (2490), Expect = 3.1e-277
Identity = 469/541 (86.69%), Postives = 497/541 (91.87%), Query Frame = 0
Query: 1 MNMNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 60
MN Y SPLI L LAFIFLSSS W A +KHESFLQCLL+HS + NYSISKVIHTPI
Sbjct: 1 MNKYYSSPLIPLFLAFIFLSSSRFWTLAVADKHESFLQCLLNHSPD-GNYSISKVIHTPI 60
Query: 61 NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDF 120
NSSYSSVLDFSIRNLRFST ETPKPLLI+TPSHVSHIQAAV+CSK YGFQIRTRSGGHDF
Sbjct: 61 NSSYSSVLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDF 120
Query: 121 EGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 180
E LSYVAHL FIIVDLINLRSIS+D+K+N AWV SGATLGELYYSIAQKSRT AFPAG+C
Sbjct: 121 ERLSYVAHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVC 180
Query: 181 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 240
PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGG
Sbjct: 181 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGG 240
Query: 241 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGK 300
SFGIVVAWKVKLVPVPATVTLC NRTLEEGAVK+THRWQYVANKLDENLFLGILWLGG
Sbjct: 241 SFGIVVAWKVKLVPVPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGN 300
Query: 301 ITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGF 360
+TSQ GGKTNPVATFFSLFLG+ADELL+ILNTKFPELDLAKKDCIETSWIESTVLMG+GF
Sbjct: 301 VTSQGGGKTNPVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGF 360
Query: 361 PTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGG 420
T++TLEALLSRTPLTN S+KIKSDYVKEP+SEA +QGI +RL++QDIE GN+IFVPYGG
Sbjct: 361 QTKVTLEALLSRTPLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFVPYGG 420
Query: 421 RMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRA 480
RMSQI ESETPFSHRAGYLYKIGYI W +Q ID EKRHL WIRELY YM PFVSKSPRA
Sbjct: 421 RMSQIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSKSPRA 480
Query: 481 AYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPA 540
YANYRDLDIGSN ++GKTSYKQAS WG KYFGNNFNRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 VYANYRDLDIGSNKRYGKTSYKQASTWGFKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 540
Query: 541 L 542
L
Sbjct: 541 L 540
BLAST of Moc03g24700 vs. ExPASy TrEMBL
Match:
A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)
HSP 1 Score: 882.9 bits (2280), Expect = 6.9e-253
Identity = 427/541 (78.93%), Postives = 480/541 (88.72%), Query Frame = 0
Query: 3 MNYPSPLI--SLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 62
M Y SPLI S SL FIFLSSSSLWA+A +KHESFL CLLDHS + SNYSISKV++TP+
Sbjct: 1 MKYSSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPD-SNYSISKVVYTPL 60
Query: 63 NSSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDF 122
NSSYSSVLDFSIRNLRFST +TPKPL I+TP HVSH+QAAV+CS+++G QIRTRSGGHDF
Sbjct: 61 NSSYSSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDF 120
Query: 123 EGLSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 182
EGLS V+HLPFII+DLINLRSI+ID +NTAWVQSGATLGELYYSIA+KSRTLAFPAG C
Sbjct: 121 EGLSCVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGAC 180
Query: 183 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 242
PTVG+GGHFSGGGYGL+LRK+GLAADNVIDAYLVDA+GKF +RE MGEDLFWAIRGGGGG
Sbjct: 181 PTVGIGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGG 240
Query: 243 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGK 302
SFGIVVAWKVKLVPVPATVT C NRTLEEGA+K+ HRWQYVANKLDENLFLGIL +GG
Sbjct: 241 SFGIVVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGN 300
Query: 303 ITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGF 362
T+Q G KTNP A+FFSLFLGKADE +ILNT FPEL LAK++C+E+SWIES L+ GF
Sbjct: 301 FTAQGGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIES-ALLSAGF 360
Query: 363 PTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGG 422
+LEALL+RTPLTN S KIKSDYVKEP+SEA ++GIW+RL S+D+ ++FVPYGG
Sbjct: 361 KIGDSLEALLNRTPLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 420
Query: 423 RMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRA 482
RMSQISESE PFSHRAG LYKIGYI W++Q +D EKRHL+WIRELY YMTPFVSKSPR+
Sbjct: 421 RMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRS 480
Query: 483 AYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPA 542
AY NYRDLDIGSNNK+GKTS+K+ASVWG KYFGNNFNRLVHVKTKVDPYDFFRHEQSIP
Sbjct: 481 AYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPT 539
BLAST of Moc03g24700 vs. ExPASy TrEMBL
Match:
A0A6J1DF27 (berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE=3 SV=1)
HSP 1 Score: 833.2 bits (2151), Expect = 6.3e-238
Identity = 402/537 (74.86%), Postives = 471/537 (87.71%), Query Frame = 0
Query: 7 SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 66
SPL LSLAFI LSSSS WA+A KHE+FLQCL HS + S SISKVI+TPIN SYSS
Sbjct: 4 SPLTILSLAFILLSSSSSWAAASAEKHEAFLQCLSHHSADSS--SISKVIYTPINPSYSS 63
Query: 67 VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 126
VL+FS+RNLRFS+ ETPKPLLI+TPS+VSH+QAAVICSK++G QIR RSGGHD EGLSYV
Sbjct: 64 VLNFSVRNLRFSSAETPKPLLIITPSYVSHVQAAVICSKTHGLQIRIRSGGHDIEGLSYV 123
Query: 127 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 186
AHLPFII+DLINL+SI+++I+++TAW+QSGATLGELYY+IAQKSRTLAFPAG+CPTVGVG
Sbjct: 124 AHLPFIIIDLINLKSITVNIENSTAWIQSGATLGELYYNIAQKSRTLAFPAGVCPTVGVG 183
Query: 187 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 246
GHFSGGGYGL+LRKYGLAADNVIDAYL+DA+GKF++RESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 184 GHFSGGGYGLMLRKYGLAADNVIDAYLIDANGKFYDRESMGEDLFWAIRGGGGGSFGIVV 243
Query: 247 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 306
AWKVKLVPVPA VT CS++R L+E + + HRWQ VA KLDENL+ GI+ GG S EG
Sbjct: 244 AWKVKLVPVPAIVTTCSSDRNLDEDTINLIHRWQSVAYKLDENLYFGIILAGGN-ASTEG 303
Query: 307 G--KTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTEL 366
G K NP ATFFSLFLGK+DEL++ILNT FPEL+LAKKDC+ETSWIEST++ T
Sbjct: 304 GVKKPNPTATFFSLFLGKSDELMAILNTNFPELNLAKKDCVETSWIESTLVAATRNQTIE 363
Query: 367 TLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQ 426
+LE+LL+RTPLT ++K+KSDYVKEP+ +A ++GIW+RL +QDIE ++FVPYGGRMS+
Sbjct: 364 SLESLLNRTPLTIENTKLKSDYVKEPIPKATIEGIWERLKAQDIEVPQLVFVPYGGRMSR 423
Query: 427 ISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYAN 486
IS+SETPFSHRA LYKIGYI GW+EQ + AE+RHL WIRE+YDYMTPFVSKSPRA Y N
Sbjct: 424 ISDSETPFSHRARNLYKIGYIVGWKEQSVSAEERHLSWIREIYDYMTPFVSKSPRATYVN 483
Query: 487 YRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
YRDLDIG+NNK+GKTSYKQASVWG KYFGNNFNRLVH+K+KVDP +FF++EQSIP L
Sbjct: 484 YRDLDIGTNNKYGKTSYKQASVWGSKYFGNNFNRLVHIKSKVDPSNFFKNEQSIPPL 537
BLAST of Moc03g24700 vs. ExPASy TrEMBL
Match:
A0A5D3C1A1 (Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1317G00110 PE=3 SV=1)
HSP 1 Score: 828.2 bits (2138), Expect = 2.0e-236
Identity = 394/539 (73.10%), Postives = 457/539 (84.79%), Query Frame = 0
Query: 3 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 62
M Y SPLI SL I +SSSS W SA P+KH++FLQCL +HS + Y ISKV+HTPINS
Sbjct: 1 MKYSSPLIPFSLILILVSSSSSWVSASPHKHQNFLQCLSEHSSK--RYPISKVVHTPINS 60
Query: 63 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 122
SYSSVLDFSIRNL FS ETPKPLLI+TPSHVSHIQAAVICSKS+G QIRTRSGGHDFEG
Sbjct: 61 SYSSVLDFSIRNLLFSKPETPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEG 120
Query: 123 LSYVAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPT 182
LSYVA+ PFIIVDLINLRSI ID++ NTAWV+SGATLGELYY I +KSRTLAFP G+CPT
Sbjct: 121 LSYVAYHPFIIVDLINLRSIKIDVETNTAWVESGATLGELYYRIGEKSRTLAFPGGVCPT 180
Query: 183 VGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSF 242
VGVGGHFSGGGYGL+LRK+GLAADNVIDAYLVDA+GK H+R+SMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKVHDRKSMGEDLFWAIRGGGGGSF 240
Query: 243 GIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKIT 302
GIVVAWK+KLV VPATVT+CS NR L+ +K+ H+WQYVANKLDENLFLGI+ GG IT
Sbjct: 241 GIVVAWKIKLVSVPATVTICSTNRNLKGDGIKLVHQWQYVANKLDENLFLGIMLTGGNIT 300
Query: 303 SQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPT 362
+ + G NP+ATFFSLFLGK D L++ L+ FPEL K+DC+ETSWIEST++ G T
Sbjct: 301 TTQEGIANPIATFFSLFLGKVDILVATLSAIFPELGFTKEDCVETSWIESTLIAATGVGT 360
Query: 363 ELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 422
+LE LL+RTP + K+KSDY KEP+S A ++G+W+RL ++DIE +IF+PYGGRM
Sbjct: 361 VESLEPLLNRTPSNLENGKMKSDYFKEPISIATIEGLWQRLKAKDIETSQLIFIPYGGRM 420
Query: 423 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 482
SQISE ETPFSHRAG LYKIGY W+EQ + A+K+H+ WIR++Y YMTPFVSKSPR+AY
Sbjct: 421 SQISEMETPFSHRAGILYKIGYFVAWKEQSLKAKKKHISWIRDIYKYMTPFVSKSPRSAY 480
Query: 483 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIPAL 542
ANYRDLDIG+N K+GKTS +QAS+WGLKYFGNNF RLV+VKTKVDPYDFFRHEQSIP L
Sbjct: 481 ANYRDLDIGANKKYGKTSVRQASIWGLKYFGNNFKRLVYVKTKVDPYDFFRHEQSIPTL 537
BLAST of Moc03g24700 vs. TAIR 10
Match:
AT4G20820.1 (FAD-binding Berberine family protein )
HSP 1 Score: 531.9 bits (1369), Expect = 5.8e-151
Identity = 269/536 (50.19%), Postives = 369/536 (68.84%), Query Frame = 0
Query: 13 SLAFIFLSSSSLW------ASAFPNKHESFLQCLLDHSLEYSNYSI-SKVIHTPINSSYS 72
S IF ++S+L SA + SFLQCL SL+ ++ +I SKVIHTP ++S+S
Sbjct: 8 SSVLIFFTTSTLLLSIPHPVSANRSNQSSFLQCL---SLQLNDSNIVSKVIHTPNDTSFS 67
Query: 73 SVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSY 132
SVL SI+N RFS + PKP+LI+TP S +Q+AV C++ +G IRTRSGGHD+EGLSY
Sbjct: 68 SVLASSIQNQRFSAPDVPKPVLILTPVQPSDVQSAVKCARRFGIHIRTRSGGHDYEGLSY 127
Query: 133 VAHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGV 192
V H PF+I+DL NLRSI++D+ + + WVQ+GAT+GELYY I +K+RTLAFPAG+CPTVGV
Sbjct: 128 VTHKPFVILDLRNLRSITVDVDNRSVWVQTGATIGELYYEIGKKNRTLAFPAGVCPTVGV 187
Query: 193 GGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIV 252
GGHFSGGGYG LLRK+GLAAD+VIDA +VDA G+ R MGED FWAIRGGGG SF +V
Sbjct: 188 GGHFSGGGYGTLLRKHGLAADHVIDARVVDARGRILERREMGEDFFWAIRGGGGSSFCVV 247
Query: 253 VAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQE 312
++WK+ L+ VP+TVT+ + + E+ A+KI HRWQ+VA+K+ ++LF+ ++ +
Sbjct: 248 LSWKIGLINVPSTVTVFNVTKFSEQSALKIIHRWQFVADKVSDDLFIRVM--------LQ 307
Query: 313 GGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELT 372
K A+F L+LG LL ++N +FPEL L + DC E SWIES + EL
Sbjct: 308 RYKNMVRASFPGLYLGSVKNLLKMVNKEFPELGLEEDDCTEMSWIESVIWF-----AELG 367
Query: 373 LEAL--LSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMS 432
E + L++ + + K KSD+V+EPM + + +W+RL + E +IF P+GG+MS
Sbjct: 368 EEPINVLTKRTRASLAFKAKSDFVQEPMPKTAISKLWRRLQEPEAEHAQLIFTPFGGKMS 427
Query: 433 QISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAYA 492
+I++ ETPF HR G +Y+I Y+ W D +++++RW+ +YD M+ FV+KSPR AY
Sbjct: 428 EIADYETPFPHRKGNIYEIQYLNYWRG---DVKEKYMRWVERVYDDMSEFVAKSPRGAYI 487
Query: 493 NYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 540
N RDLD+G ++ Y++ WG+KYF NNF RLV VKT VDP DFF EQSIP
Sbjct: 488 NLRDLDLGMYVGVKRSKYEEGKSWGVKYFKNNFERLVRVKTSVDPSDFFCDEQSIP 524
BLAST of Moc03g24700 vs. TAIR 10
Match:
AT5G44440.1 (FAD-binding Berberine family protein )
HSP 1 Score: 530.4 bits (1365), Expect = 1.7e-150
Identity = 268/529 (50.66%), Postives = 368/529 (69.57%), Query Frame = 0
Query: 17 IFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSIS-KVIHTPINSSYSSVLDFSIRNL 76
+F + S SA + HE FL+CL S ++ ++ KVIHT +SS+ S+LD SI+N
Sbjct: 12 LFSLNISPLVSAHGSNHEDFLKCL---SYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNP 71
Query: 77 RFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYVAH-LPFIIV 136
RFS ETPKP+ I+TP S +Q + C++ +G +RTRS GH +EGLSY+A+ PF ++
Sbjct: 72 RFSVSETPKPVSIITPVKASDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVI 131
Query: 137 DLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVGGHFSGGGY 196
DL NLRSIS+D+ + T WVQ+GAT GELYY I + +++LAFPAGI PTVGVGG FSGGGY
Sbjct: 132 DLRNLRSISLDVDNRTGWVQTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGY 191
Query: 197 GLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVP 256
G LLRKYGLAADN+IDA +VDASG+ +R++MGED FWAIRGGGG SFG++++WKVKLV
Sbjct: 192 GTLLRKYGLAADNIIDALVVDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVD 251
Query: 257 VPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEGGKTNPVAT 316
VP+T+T+ +T ++ AV+I +WQY A+K+ ++LF+ + T + K A
Sbjct: 252 VPSTITVFKVQKTSKKEAVRIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHAL 311
Query: 317 FFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTLEALLSRTP 376
F L++G + LL+++ KFPEL L K+ C E SWIES VL FP +L L +R
Sbjct: 312 FTGLYIGPVNNLLALMEEKFPELGLEKEGCEEMSWIES-VLWFADFPKGESLGVLTNR-E 371
Query: 377 LTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRMSQISESETPFSH 436
T+ S K K D+V+EP+ EA +Q IW+RL++ + G II P+GG+MS+++E ETPF H
Sbjct: 372 RTSLSFKGKDDFVQEPIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPH 431
Query: 437 RAGYLYKIGYIAGWEEQGIDAEK----RHLRWIRELYDYMTPFVSKSPRAAYANYRDLDI 496
R G LY+I Y+A W E+ D K ++L+W+ +Y++MTP+VSKSPR AY N++D+D+
Sbjct: 432 RGGNLYEIQYVAYWREEE-DKNKTETDKYLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDL 491
Query: 497 GSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQSIP 540
G KT Y++ WG+KYF NNF RLV VKT+VDP DFF EQSIP
Sbjct: 492 GMYLGKKKTKYEEGKSWGVKYFKNNFERLVRVKTRVDPTDFFCDEQSIP 527
BLAST of Moc03g24700 vs. TAIR 10
Match:
AT5G44400.1 (FAD-binding Berberine family protein )
HSP 1 Score: 524.2 bits (1349), Expect = 1.2e-148
Identity = 267/550 (48.55%), Postives = 370/550 (67.27%), Query Frame = 0
Query: 1 MNMNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPI 60
M ++ P PL S+ + + L + + S+ + + F+ C+ ++ Y + + K P
Sbjct: 1 MGISKPLPLFSILVLYFSLYTITP-TSSLASLQDQFINCVQRNTHVY--FPLEKTFFAPT 60
Query: 61 N--SSYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGH 120
S +S VL+ + +NLRF PKP I +P H SH+QA++ICSK +R RSGGH
Sbjct: 61 KNVSMFSQVLESTAQNLRFLKKSMPKPGFIFSPIHESHVQASIICSKKLRMHLRVRSGGH 120
Query: 121 DFEGLSYVAHL--PFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFP 180
D+EGLSYV+ + PFI++DL +R ++I+I+DN+AWVQSGAT+GELYY IA+KS+ FP
Sbjct: 121 DYEGLSYVSQIDKPFILMDLSKMRQVNINIQDNSAWVQSGATVGELYYRIAEKSKVHGFP 180
Query: 181 AGICPTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRG 240
AG+C ++G+GGH +GG YG ++RKYGL ADNV+DA +VDA+GK +R +MGED FWAIRG
Sbjct: 181 AGLCSSLGIGGHITGGAYGSMMRKYGLGADNVLDAKIVDANGKLLDRAAMGEDTFWAIRG 240
Query: 241 GGGGSFGIVVAWKVKLVPVPATVTLCSANRTLEEG-AVKITHRWQYVANKLDENLFLGIL 300
G GGSFGI++AWK+KLVPVP TVT+ + +TL++ KI +WQ VA+KL E LF+ +L
Sbjct: 241 GAGGSFGIILAWKIKLVPVPKTVTVFTVTKTLQQDVGNKIISKWQRVADKLVEELFIRVL 300
Query: 301 WLGGKITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVL 360
+ + GG ++ +LFLG L++++ FPEL L KDCIE SW+ES
Sbjct: 301 F----NVAGTGGNKTVTTSYNALFLGGKGTLMNVMKKSFPELGLTFKDCIEMSWLESIAY 360
Query: 361 MGVGFPTELTLEALL-SRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNII 420
+ GFPT LL ++P S K KSD+VK P+ E+ +QGI+K+L +DI +I
Sbjct: 361 IS-GFPTHTPTNVLLQGKSPFPKVSFKAKSDFVKTPIPESGLQGIFKKLLKEDIP--LMI 420
Query: 421 FVPYGGRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEK---RHLRWIRELYDYMT 480
+ PYGG M++I ES+ PF HR G L+K+ Y+ W +D++K RH+ WIR+LY YMT
Sbjct: 421 WNPYGGMMAKIPESQIPFPHRKGVLFKVQYVTSW----LDSDKRPSRHINWIRDLYSYMT 480
Query: 481 PFVSKSPRAAYANYRDLDIGSNNKFGKTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDF 540
P+VS +PR AY NYRDLD+G N K KT KQA VWG YF NNFNRL+ +K KVDP +F
Sbjct: 481 PYVSSNPREAYVNYRDLDLGRNTKDVKTCIKQAQVWGANYFKNNFNRLMMIKAKVDPENF 536
Query: 541 FRHEQSIPAL 542
FRHEQSIP +
Sbjct: 541 FRHEQSIPPM 536
BLAST of Moc03g24700 vs. TAIR 10
Match:
AT5G44410.1 (FAD-binding Berberine family protein )
HSP 1 Score: 515.8 bits (1327), Expect = 4.3e-146
Identity = 259/544 (47.61%), Postives = 372/544 (68.38%), Query Frame = 0
Query: 9 LISLSLAFIFLSSSSLWASAFPN------KHESFLQCLLDHSLEYSNYSISKVIHTPINS 68
L+SL + F+ L+ S S FP+ HE+FL+C L H + N S++IHT +
Sbjct: 7 LLSLFIYFLLLNLS---LSHFPSISAQRTNHENFLKC-LSHRI---NEDDSRIIHTSKDP 66
Query: 69 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 128
SY S+L+ SI+N RF +ETPKP+ I+TP + +Q+ + C++ +G IRTRSGGHD+EG
Sbjct: 67 SYFSILNSSIQNPRFFVLETPKPVSIITPVQATDVQSTIKCARLHGIHIRTRSGGHDYEG 126
Query: 129 LSYVA-HLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICP 188
LSY+A PF+++DL NLRSI++D+ + T WVQSGAT+GELYY I + S++LAFPAG+ P
Sbjct: 127 LSYMAKSRPFVVIDLRNLRSITLDVDNRTGWVQSGATIGELYYEIGKLSKSLAFPAGLYP 186
Query: 189 TVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGS 248
TVG+GG F GGGYG L+RKYGL+ADNVIDA++VDA+G F +R+ MGED FWAIRGGGG S
Sbjct: 187 TVGIGGQFGGGGYGTLMRKYGLSADNVIDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSS 246
Query: 249 FGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKI 308
F +V++WK++L+ VP+ VT+ +T E+ AV I ++WQY+A+K+ +LF I
Sbjct: 247 FSVVLSWKIRLLDVPSVVTVFKVVKTSEKEAVSIINKWQYIADKVPNDLF---------I 306
Query: 309 TSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFP 368
+ +T A+F L+LG +LL+++ KFPEL L +C E SWIES + G
Sbjct: 307 RAMLQKETEVYASFPGLYLGPVSDLLALMKDKFPELGLEIGNCREMSWIESVLWFIKGES 366
Query: 369 TELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGR 428
E +L++ T+ S K K D+++EP+ + +Q +W+R ++ + II P+GG+
Sbjct: 367 ME-----ILAKRKRTSRSFKGKDDFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGK 426
Query: 429 MSQISESETPFSHRAGYLYKIGYIAGW---EEQGIDAEKRHLRWIRELYDYMTPFVSKSP 488
MS+I+++E PF HR G LY+I Y+A W E++ +++LRW+ +Y++MTP+VSKSP
Sbjct: 427 MSEIADNEIPFPHREGNLYEIQYLAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSP 486
Query: 489 RAAYANYRDLDIGSNNKFG-KTSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQS 542
R AY N+RD+D+G KT Y++A VWG+KYF NNF+RLV VKT VDP DFF EQS
Sbjct: 487 RRAYVNFRDIDLGMYLGLNMKTKYEEAKVWGVKYFKNNFDRLVRVKTNVDPMDFFCDEQS 529
BLAST of Moc03g24700 vs. TAIR 10
Match:
AT1G30700.1 (FAD-binding Berberine family protein )
HSP 1 Score: 510.8 bits (1314), Expect = 1.4e-144
Identity = 273/544 (50.18%), Postives = 363/544 (66.73%), Query Frame = 0
Query: 3 MNYPSPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINS 62
M Y L+ + FI+ SSSS S E+F QCL +S + IS I N
Sbjct: 1 MKYALILVLFFVVFIWQSSSSSANS------ETFTQCLTSNS--DPKHPISPAIFFSGNG 60
Query: 63 SYSSVLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEG 122
SYSSVL +IRNLRF+T TPKP LI+ +H SH+QAA+ C K + Q++ RSGGHD++G
Sbjct: 61 SYSSVLQANIRNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDG 120
Query: 123 LSYVAH--LPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGIC 182
LSYV + PF ++D+ NLRS+ +D+ TAWVQ+GA LGE+YY I +KS+TLA+PAGIC
Sbjct: 121 LSYVTYSGKPFFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGIC 180
Query: 183 PTVGVGGHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGG 242
PTVGVGGH SGGGYG ++RKYGL DN IDA +VD +GK +R+ MGEDL+WAI GGGGG
Sbjct: 181 PTVGVGGHISGGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGG 240
Query: 243 SFGIVVAWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLG--ILWLG 302
S+G+V+A+K+ LV VP VT+ +RTLE+ A I HRWQ VA KL + LF+ I +
Sbjct: 241 SYGVVLAYKINLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVN 300
Query: 303 GKITSQEGGKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGV 362
G ++SQ+ +T TF ++FLG LLSILN +FPEL L + DC ETSWI+S VL
Sbjct: 301 GTVSSQKTVRT----TFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQS-VLFWT 360
Query: 363 GFPTELTLEALLSRTPLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPY 422
+ LL R N K KSDYV+EP+S ++ IWK++ ++E + F PY
Sbjct: 361 NIQVGSSETLLLQRNQPVN-YLKRKSDYVREPISRTGLESIWKKM--IELEIPTMAFNPY 420
Query: 423 GGRMSQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSP 482
GG M +IS + TPF +RAG L+KI Y A W ++ + R++ R+LY +MTPFVSK+P
Sbjct: 421 GGEMGRISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNP 480
Query: 483 RAAYANYRDLDIGSNNKFGK-TSYKQASVWGLKYFGNNFNRLVHVKTKVDPYDFFRHEQS 542
R ++ NYRD+D+G N+ GK +SY + +G KYF NF RLV +KT+VD +FFR+EQS
Sbjct: 481 RQSFFNYRDVDLGINSHNGKISSYVEGKRYGKKYFAGNFERLVKIKTRVDSGNFFRNEQS 526
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_022142587.1 | 0.0e+00 | 100.00 | tetrahydrocannabinolic acid synthase-like [Momordica charantia] | [more] |
| XP_022142425.1 | 6.4e-277 | 86.69 | berberine bridge enzyme-like 18 [Momordica charantia] | [more] |
| XP_022152516.1 | 1.4e-252 | 78.93 | berberine bridge enzyme-like 18 [Momordica charantia] | [more] |
| XP_038895508.1 | 7.9e-243 | 76.25 | berberine bridge enzyme-like 28 [Benincasa hispida] | [more] |
| XP_004151192.3 | 2.1e-240 | 74.77 | berberine bridge enzyme-like 18 [Cucumis sativus] >KAE8653316.1 hypothetical pro... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| Q8GTB6 | 1.8e-152 | 50.84 | Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV... | [more] |
| A6P6V9 | 2.8e-150 | 50.94 | Cannabidiolic acid synthase OS=Cannabis sativa OX=3483 GN=CBDAS PE=1 SV=1 | [more] |
| Q9SVG5 | 8.1e-150 | 50.19 | Berberine bridge enzyme-like 18 OS=Arabidopsis thaliana OX=3702 GN=At4g20820 PE=... | [more] |
| Q9FI21 | 2.4e-149 | 50.66 | Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=... | [more] |
| Q33DQ2 | 2.4e-149 | 50.28 | Cannabichromenic acid synthase OS=Cannabis sativa OX=3483 GN=CBCAS PE=1 SV=1 | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1CN68 | 0.0e+00 | 100.00 | tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC1... | [more] |
| A0A6J1CKW8 | 3.1e-277 | 86.69 | berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 P... | [more] |
| A0A6J1DHZ2 | 6.9e-253 | 78.93 | berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 P... | [more] |
| A0A6J1DF27 | 6.3e-238 | 74.86 | berberine bridge enzyme-like 8 OS=Momordica charantia OX=3673 GN=LOC111020224 PE... | [more] |
| A0A5D3C1A1 | 2.0e-236 | 73.10 | Tetrahydrocannabinolic acid synthase-like OS=Cucumis melo var. makuwa OX=1194695... | [more] |
