Moc03g23150 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g23150 vs. NCBI nr
Match: XP_022142562.1 (subtilisin-like protease SBT5.4 [Momordica charantia])

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 779/779 (100.00%), Postives = 779/779 (100.00%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60
           MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL
Sbjct: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60

Query: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120
           GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW
Sbjct: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120

Query: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180
           NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180

Query: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240
           TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240

Query: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300
           SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300

Query: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360
           LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360

Query: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420
           QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420

Query: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480
           KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480

Query: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540
           VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540

Query: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600
           YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600

Query: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660
           IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660

Query: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720
           KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720

Query: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of Moc03g23150 vs. NCBI nr
Match: XP_038895602.1 (subtilisin-like protease SBT5.4 [Benincasa hispida])

HSP 1 Score: 1295.0 bits (3350), Expect = 0.0e+00
Identity = 632/779 (81.13%), Postives = 707/779 (90.76%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60
           MK+SNSIL +FS    F LLIS AIAAKKSYVV+LGSHSHG+E++E DL+RVV SHHKLL
Sbjct: 1   MKISNSILILFS----FLLLISSAIAAKKSYVVLLGSHSHGIEITEEDLERVVHSHHKLL 60

Query: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120
           GSFLGS EKAKDAIFYSYKK+INGFAA L+EEEA +LA+HPEVAA+LAN+ K LHTTHSW
Sbjct: 61  GSFLGSEEKAKDAIFYSYKKHINGFAATLDEEEATKLAEHPEVAAILANRAKALHTTHSW 120

Query: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180
            FM LEK NGV+P +SPW  A  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGC
Sbjct: 121 EFMHLEK-NGVIPSSSPWRIAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGC 180

Query: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240
            D++PDRVPCNRKLIGAKYFNKGFIAYLK+ NS+ + SS+ NSTRDYEGHGSHTLSTAGG
Sbjct: 181 MDETPDRVPCNRKLIGAKYFNKGFIAYLKSENST-EFSSVINSTRDYEGHGSHTLSTAGG 240

Query: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300
           S+V  ASVFGSG GTAKGGSPKARVAAYK+CWP + GGCFD+DI E FDHAIHDGV+V+S
Sbjct: 241 SYVSGASVFGSGAGTAKGGSPKARVAAYKVCWPLEGGGCFDSDIVEAFDHAIHDGVDVLS 300

Query: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360
           LS+G  PA Y +DSIAIAAFHAVKKGIPVVCSAGNSGPDA TASNTAPWILTVGASTLDR
Sbjct: 301 LSLGSDPAEYSEDSIAIAAFHAVKKGIPVVCSAGNSGPDALTASNTAPWILTVGASTLDR 360

Query: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420
           +FQAPVEL+NG R+ GSSLS AL  +KLYPLITGAQAKA+NA+  DAMLCKPETLDHSK 
Sbjct: 361 EFQAPVELQNGHRYMGSSLSKALQGNKLYPLITGAQAKAKNAAMDDAMLCKPETLDHSKV 420

Query: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480
           KGKILACLRGDNARVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQA 480

Query: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540
           VL YI ST+NPMGYLIPP+AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVNVIAA
Sbjct: 481 VLSYINSTKNPMGYLIPPSAKVNTKPAPTMAAFSSRGPNVISPEIIKPDVTAPGVNVIAA 540

Query: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600
           ++EAVSPTGE FDNRT PFITMSGTSMSCPHV+GL GLLR+LHP+WSPSAIKSAIMTSAR
Sbjct: 541 FSEAVSPTGEAFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSAR 600

Query: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660
           IRDNTMKPMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GYD+
Sbjct: 601 IRDNTMKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYDD 660

Query: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720
           K IRAFSDGP+KCPP+AS+LNFNYPSIGVQN+ G+VTVTR+LKNV TPGVYRAR+  P+G
Sbjct: 661 KTIRAFSDGPFKCPPNASILNFNYPSIGVQNLNGNVTVTRKLKNVSTPGVYRARIMNPDG 720

Query: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           V+VSV+P+ LKF++V EEKSF+LT+ G VP  +VVDG LIWTDG+HFVRSPIVISS LF
Sbjct: 721 VKVSVKPKVLKFERVAEEKSFELTMTGNVPEDQVVDGVLIWTDGKHFVRSPIVISSVLF 773

BLAST of Moc03g23150 vs. NCBI nr
Match: XP_008464322.1 (PREDICTED: subtilisin-like protease SBT5.4 [Cucumis melo])

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 612/779 (78.56%), Postives = 694/779 (89.09%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60
           MK+ NSIL +FS L    L+ISPAIAAKKSY+V+LGSHSHGLEV + DL+RV DSHHKLL
Sbjct: 6   MKIYNSILILFSLL----LVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLL 65

Query: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120
           GS +GS EKA+DAIFYSYK+NINGFAAI++EEEA +LAKHPEVAAVL NK KKLHTTHSW
Sbjct: 66  GSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSW 125

Query: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180
            FM LEK NGV+PP+S W RA  G+D II NLDTGVW ESKSFGE+GIVG+VP+KWKGGC
Sbjct: 126 EFMHLEK-NGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGC 185

Query: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240
           TD +PD V CNRKLIGAKYFNKGF+AYL + N +A   S+ NSTRDY+GHGSHTLSTAGG
Sbjct: 186 TDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTA---SVINSTRDYDGHGSHTLSTAGG 245

Query: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300
           S+V  ASVFG G+GTAKGGSPKARVA+YK+CWP +DGGCF+ADI E FDHAIHD V+V+S
Sbjct: 246 SYVSGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLS 305

Query: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360
           LS+GG PA+Y+DD IAIAAFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGASTLDR
Sbjct: 306 LSLGGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDR 365

Query: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420
           +FQAPVEL+NG  + GSSLS  L  DKLYPLITGA+AKA+NA+A  AMLCKP+TLDHSK 
Sbjct: 366 EFQAPVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKV 425

Query: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480
           KGKIL CLRGD ARVDKGEQAALAGA GMILCND+LSGFETIADPH+LPASH+NYNDGQA
Sbjct: 426 KGKILVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQA 485

Query: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540
           V  YI+ST+NPMG LIPP+AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA
Sbjct: 486 VFSYIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAA 545

Query: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600
           ++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP+WSPSAIKSAIMTSAR
Sbjct: 546 FSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSAR 605

Query: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660
           IRDNT KPMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+E
Sbjct: 606 IRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNE 665

Query: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720
           K I+AFSDGP+KCP SAS+LNFNYPSIGVQN+TGSVT+TR+LKNV TPGVY+ARV  P G
Sbjct: 666 KTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNG 725

Query: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           V+V V+P+ LKF++VGEEK F+L I G VP  +VVDG LIWTDG+HFVRSPIV+SSGLF
Sbjct: 726 VKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of Moc03g23150 vs. NCBI nr
Match: KAE8652826.1 (hypothetical protein Csa_022873 [Cucumis sativus])

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 600/757 (79.26%), Postives = 680/757 (89.83%), Query Frame = 0

Query: 25  IAAKK--SYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKKNI 84
           +AAKK  SY+V+LGSHSHGLEV++ DL+RV DSHHKLLGS  GS EKA++AIFYSYKKNI
Sbjct: 1   MAAKKNISYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNAIFYSYKKNI 60

Query: 85  NGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNGVVPPTSPWWRAN 144
           NGFAAI++EEEAA+LAKHPEVAAVL N+ KKLHTTHSW FM LEK NGV+PP+S W RA 
Sbjct: 61  NGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK-NGVIPPSSAWRRAK 120

Query: 145 FGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRKLIGAKYFNK 204
            G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGCTD + DRVPCNRKLIGAKYFNK
Sbjct: 121 SGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRKLIGAKYFNK 180

Query: 205 GFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFGSGLGTAKGGSPK 264
           GF+AYLK+ N +A    + NSTRDY+GHGSHTLSTAGGS+V  ASVFG G+GTAKGGSPK
Sbjct: 181 GFLAYLKSENLTA---LVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGVGTAKGGSPK 240

Query: 265 ARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDDSIAIAAFHA 324
           ARVAAYK+CWP +DGGCFDADI + FDHAIHD V+V+SLS+GG PA+Y+DD IAI+AFHA
Sbjct: 241 ARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDDGIAISAFHA 300

Query: 325 VKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQRFQGSSLSTA 384
           VKKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DR+FQAPVEL+NG R+ GSSLS  
Sbjct: 301 VKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHRYMGSSLSKG 360

Query: 385 LPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNARVDKGEQAA 444
           L  DKLYPLITGA+AKA+NA+A +A LCKP+TLDHSK KGKIL CLRGD ARVDKGEQAA
Sbjct: 361 LKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTARVDKGEQAA 420

Query: 445 LAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMGYLIPPAAKL 504
           LAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQAV  YI++T+NPMGYLIPP AK+
Sbjct: 421 LAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMGYLIPPTAKV 480

Query: 505 NTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITM 564
           NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA++EAVSPTGEPFDNRT PFITM
Sbjct: 481 NTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFDNRTVPFITM 540

Query: 565 SGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATP 624
           SGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSARIRDNT KPMLDGG+ +LAP+TP
Sbjct: 541 SGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGGSPDLAPSTP 600

Query: 625 FSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNF 684
           F+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+EK I+AFSDGP+KCP SAS+LN 
Sbjct: 601 FAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKCPASASILNL 660

Query: 685 NYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFK 744
           NYPSIGVQN+TGSVTVTR+LKNV TPGVY+ RVR P GV+V V+P+ LKF++VGEEKSF+
Sbjct: 661 NYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFERVGEEKSFE 720

Query: 745 LTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 LTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 753

BLAST of Moc03g23150 vs. NCBI nr
Match: XP_031739111.1 (subtilisin-like protease SBT5.4 [Cucumis sativus])

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 599/766 (78.20%), Postives = 683/766 (89.16%), Query Frame = 0

Query: 14  LLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDA 73
           ++LF L++       +SY+V+LGSHSHGLEV++ DL+RV DSHHKLLGS  GS EKA++A
Sbjct: 1   MVLFCLML-----GFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNA 60

Query: 74  IFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNGVVP 133
           IFYSYKKNINGFAAI++EEEAA+LAKHPEVAAVL N+ KKLHTTHSW FM LEK NGV+P
Sbjct: 61  IFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK-NGVIP 120

Query: 134 PTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRK 193
           P+S W RA  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGCTD + DRVPCNRK
Sbjct: 121 PSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRK 180

Query: 194 LIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFGSGL 253
           LIGAKYFNKGF+AYLK+ N +A    + NSTRDY+GHGSHTLSTAGGS+V  ASVFG G+
Sbjct: 181 LIGAKYFNKGFLAYLKSENLTA---LVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGV 240

Query: 254 GTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDD 313
           GTAKGGSPKARVAAYK+CWP +DGGCFDADI + FDHAIHD V+V+SLS+GG PA+Y+DD
Sbjct: 241 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDD 300

Query: 314 SIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQR 373
            IAI+AFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DR+FQAPVEL+NG R
Sbjct: 301 GIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHR 360

Query: 374 FQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNA 433
           + GSSLS  L  DKLYPLITGA+AKA+NA+A +A LCKP+TLDHSK KGKIL CLRGD A
Sbjct: 361 YMGSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTA 420

Query: 434 RVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMG 493
           RVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQAV  YI++T+NPMG
Sbjct: 421 RVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMG 480

Query: 494 YLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFD 553
           YLIPP AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA++EAVSPTGEPFD
Sbjct: 481 YLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFD 540

Query: 554 NRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGG 613
           NRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSARIRDNT KPMLDGG
Sbjct: 541 NRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGG 600

Query: 614 TDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKC 673
           + +LAP+TPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+EK I+AFSDGP+KC
Sbjct: 601 SPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKC 660

Query: 674 PPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFD 733
           P SAS+LN NYPSIGVQN+TGSVTVTR+LKNV TPGVY+ RVR P GV+V V+P+ LKF+
Sbjct: 661 PASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFE 720

Query: 734 KVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           +VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 RVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of Moc03g23150 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 936.8 bits (2420), Expect = 1.6e-271
Identity = 473/772 (61.27%), Postives = 581/772 (75.26%), Query Frame = 0

Query: 6   SILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLG 65
           S+ ++ S LLL +L  SPA A KKSY+V LGSH+H  ++S A L  V  SH   L SF+G
Sbjct: 17  SLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 76

Query: 66  SSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRL 125
           S E AK+AIFYSYK++INGFAAIL+E EAAE+AKHP+V +V  NKG+KLHTTHSWNFM L
Sbjct: 77  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136

Query: 126 EKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSP 185
            K NGVV  +S W +A +G+DTIIANLDTGVWPESKSF + G  G+VP +WKG C  D  
Sbjct: 137 AK-NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD-- 196

Query: 186 DRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPD 245
             VPCNRKLIGA+YFNKG++AY   L S+A       + RD++GHGSHTLSTA G+FVP 
Sbjct: 197 --VPCNRKLIGARYFNKGYLAY-TGLPSNASY----ETCRDHDGHGSHTLSTAAGNFVPG 256

Query: 246 ASVFGSGLGTAKGGSPKARVAAYKICWPFQDGG-CFDADITEGFDHAIHDGVEVISLSVG 305
           A+VFG G GTA GGSPKARVAAYK+CWP  DG  CFDADI    + AI DGV+V+S SVG
Sbjct: 257 ANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVG 316

Query: 306 GSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQA 365
           G   +Y  D IAI +FHAVK G+ VVCSAGNSGP + T SN APW++TVGAS++DR+FQA
Sbjct: 317 GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQA 376

Query: 366 PVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKI 425
            VELKNGQ F+G+SLS  LPE+K+Y LI+ A A   N +  DA+LCK  +LD  K KGKI
Sbjct: 377 FVELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 436

Query: 426 LACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKY 485
           L CLRGDNARVDKG QAA AGAAGM+LCND+ SG E I+D H+LPAS ++Y DG+ +  Y
Sbjct: 437 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 496

Query: 486 IQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEA 545
           + ST++P GY+  P A LNTKPAP MA+FSSRGPN +TP I+KPD+TAPGVN+IAA+TEA
Sbjct: 497 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 556

Query: 546 VSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDN 605
             PT    DNR  PF T SGTSMSCPH++G+ GLL++LHP+WSP+AI+SAIMT++R R+N
Sbjct: 557 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 616

Query: 606 TMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIR 665
             KPM+D   +    A PFSYGSGH++P  A  PGLVYDLT  DYL+FLCA+GY+   ++
Sbjct: 617 RRKPMVD---ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQ 676

Query: 666 AFSDGP-YKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRV 725
            F++ P Y C   A+LL+FNYPSI V N+TGS+TVTR+LKNVG P  Y AR R+P GVRV
Sbjct: 677 LFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRV 736

Query: 726 SVEPRFLKFDKVGEEKSFKLTIAGV-VPAKRVVDGTLIWTDGEHFVRSPIVI 775
           SVEP+ L F+K GE K F++T+  + V     V G L WTD  H+VRSPIV+
Sbjct: 737 SVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of Moc03g23150 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 4.8e-236
Identity = 434/784 (55.36%), Postives = 554/784 (70.66%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKK---SYVVILGSHSHGLEVSEADLQRVVDSHH 60
           MKL+++    F  LLL   + S  I A K   SYVV  G+HSH  E++E  + RV ++H+
Sbjct: 1   MKLTHNF--SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHY 60

Query: 61  KLLGSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTT 120
             LGSF GS E+A DAIFYSY K+INGFAA L+ + A E++KHPEV +V  NK  KLHTT
Sbjct: 61  DFLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTT 120

Query: 121 HSWNFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWK 180
            SW+F+ LE  N  VP +S W +A FG+DTIIANLDTGVWPESKSF + G+ G +P++WK
Sbjct: 121 RSWDFLGLEH-NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWK 180

Query: 181 GGCTDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLST 240
           G C +       CNRKLIGA+YFNKG+ A +  LNSS D      S RD +GHGSHTLST
Sbjct: 181 GICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLST 240

Query: 241 AGGSFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGG-CFDADITEGFDHAIHDGV 300
           A G FVP  S+FG G GTAKGGSP+ARVAAYK+CWP   G  C+DAD+   FD AIHDG 
Sbjct: 241 AAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGA 300

Query: 301 EVISLSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGAS 360
           +VIS+S+GG P ++F+DS+AI +FHA KK I VVCSAGNSGP   T SN APW +TVGAS
Sbjct: 301 DVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAS 360

Query: 361 TLDRQFQAPVELKNGQRFQGSSL-STALPEDKLYPLITGAQAKAENASAADAMLCKPETL 420
           T+DR+F + + L NG+ ++G SL STALP  K YP++    AKA+NASA DA LCK  +L
Sbjct: 361 TMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSL 420

Query: 421 DHSKAKGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNY 480
           D  K KGKIL CLRG N RV+KG   AL G  GM+L N  ++G + +ADPH+LPA+ +  
Sbjct: 421 DPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTS 480

Query: 481 NDGQAVLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGV 540
            D  AV +YI  T+ P+ ++ P    L  KPAPVMA+FSS+GP+IV P+I+KPD+TAPGV
Sbjct: 481 KDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGV 540

Query: 541 NVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAI 600
           +VIAAYT AVSPT E FD R   F  +SGTSMSCPH++G+AGLL++ +P+WSP+AI+SAI
Sbjct: 541 SVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAI 600

Query: 601 MTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCA 660
           MT+A I D+   P+ +        ATPFS+G+GH++P  AV+PGLVYDL   DYL FLC+
Sbjct: 601 MTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCS 660

Query: 661 IGYDEKKIRAFSDGPYKC-PPSASLLNFNYPSIGVQNMTGS-VTVTRRLKNVGTPGVYRA 720
           +GY+  +I  FS   + C  P  SL+N NYPSI V N+T S VTV+R +KNVG P +Y  
Sbjct: 661 LGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTV 720

Query: 721 RVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA---GVVPAKRVVDGTLIWTDGEHFVRS 775
           +V  P+GV V+V+P  L F KVGE+K+FK+ +    G V AK  V G L+W+D +H VRS
Sbjct: 721 KVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV-AKGYVFGELVWSDKKHRVRS 770

BLAST of Moc03g23150 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 668.3 bits (1723), Expect = 1.0e-190
Identity = 375/792 (47.35%), Postives = 494/792 (62.37%), Query Frame = 0

Query: 10  IFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEK 69
           I SS  LF+ L++    +KK Y+V +G+HSHG   + ADL+   DSH+ LLGS  GS EK
Sbjct: 11  ILSSFFLFTFLLAAVNGSKKCYIVYMGAHSHGPSPTSADLELATDSHYDLLGSIFGSREK 70

Query: 70  AKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMN 129
           AK+AI YSY ++INGFAA+LEEEEAA++AK+P V +V  +K  KLHTT SW F+ L +  
Sbjct: 71  AKEAIIYSYNRHINGFAALLEEEEAADIAKNPNVVSVFLSKEHKLHTTRSWEFLGLHRRG 130

Query: 130 GVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTD----DSP 189
                 S W +  FG++TII N+DTGVWPES+SF + G  G+VP+KW+GG          
Sbjct: 131 ----QNSAWQKGRFGENTIIGNIDTGVWPESQSFSDKG-YGTVPSKWRGGLCQINKLPGS 190

Query: 190 DRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPD 249
            +  CNRKLIGA+Y+NK F A+      +  L  + ++ RD+ GHG+HTLSTAGG+FVP 
Sbjct: 191 MKNTCNRKLIGARYYNKAFEAH------NGQLDPLLHTARDFVGHGTHTLSTAGGNFVPG 250

Query: 250 ASVFGSGLGTAKGGSPKARVAAYKICWPFQD-GGCFDADITEGFDHAIHDGVEVISLSVG 309
           A VF  G GTAKGGSP+ARVAAYK+CW   D   C+ AD+    D AI DGV+VI++S G
Sbjct: 251 ARVFAVGNGTAKGGSPRARVAAYKVCWSLTDPASCYGADVLAAIDQAIDDGVDVINVSFG 310

Query: 310 GS----PANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 369
            S        F D I+I AFHA+ K I +V SAGN GP   T +N APW+ T+ ASTLDR
Sbjct: 311 VSYVVTAEGIFTDEISIGAFHAISKNILLVASAGNDGPTPGTVANVAPWVFTIAASTLDR 370

Query: 370 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 429
            F + + + N Q  +G+SL   LP ++ + LI    AK  NA+  DA LC+  TLD +K 
Sbjct: 371 DFSSNLTI-NNQLIEGASLFVNLPPNQAFSLILSTDAKLANATFRDAQLCRRGTLDRTKV 430

Query: 430 KGKILACLR-GDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQ 489
            GKI+ C R G    V +G +A  AGA GMIL N   +G    A+PH+   S VN    +
Sbjct: 431 NGKIVLCTREGKIKSVAEGLEALTAGARGMILNNQMQNGKTLSAEPHVF--STVNTPPRR 490

Query: 490 AVLKYIQSTQNPMGYLIPPAAKLNT------------KPAPVMAAFSSRGPNIVTPEIIK 549
           A  +        +G    P    +T            KPAPVMA+FSSRGPN + P I+K
Sbjct: 491 AKSRPHGVKTTAIGDEDDPLKTGDTIKMSRARTLFGRKPAPVMASFSSRGPNKIQPSILK 550

Query: 550 PDVTAPGVNVIAAYTEAVSPTGEPFDNRTA-PFITMSGTSMSCPHVAGLAGLLRSLHPNW 609
           PDVTAPGVN++AAY+E  S +    DNR    F  + GTSMSCPH +G+AGLL++ HP+W
Sbjct: 551 PDVTAPGVNILAAYSEFASASSLLVDNRRGFKFNVLQGTSMSCPHASGIAGLLKTRHPSW 610

Query: 610 SPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTP 669
           SP+AIKSAIMT+A   DNT +P+ D     LA A  F+YGSGH+RP  A++PGLVYDL+ 
Sbjct: 611 SPAAIKSAIMTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSL 670

Query: 670 DDYLEFLCAIGYDEKKIRAFS-DGPYKCPPSASLLNFNYPSIGVQNM-TGSVTVTRRLKN 729
            DYL FLCA GYD++ I A + +  + C  S S+ + NYPSI + N+    VT+ R + N
Sbjct: 671 TDYLNFLCASGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTN 730

Query: 730 VGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTI--AGVVPAKRVVDGTLIWT 775
           VG P  Y    R P G  ++V P  L F K+GE K+FK+ +  +     ++   G L WT
Sbjct: 731 VGPPSTYTVSTRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWT 786

BLAST of Moc03g23150 vs. ExPASy Swiss-Prot
Match: O65351 (Subtilisin-like protease SBT1.7 OS=Arabidopsis thaliana OX=3702 GN=SBT1.7 PE=1 SV=1)

HSP 1 Score: 575.5 bits (1482), Expect = 9.2e-163
Identity = 336/779 (43.13%), Postives = 463/779 (59.44%), Query Frame = 0

Query: 6   SILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLG 65
           S  A F  L L    +S + + + +Y+V          ++++ +    D H     S L 
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSLR 66

Query: 66  SSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRL 125
           S   + + + Y+Y+  I+GF+  L +EEA  L   P V +VL     +LHTT +  F+ L
Sbjct: 67  SISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 126

Query: 126 EKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSP 185
           ++    + P      A    D ++  LDTGVWPESKS+ + G  G +P+ WKGGC   + 
Sbjct: 127 DEHTADLFP-----EAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTN 186

Query: 186 DRVP-CNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVP 245
                CNRKLIGA++F +G+ + +  ++ S +      S RD +GHG+HT STA GS V 
Sbjct: 187 FTASLCNRKLIGARFFARGYESTMGPIDESKE----SRSPRDDDGHGTHTSSTAAGSVVE 246

Query: 246 DASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVG 305
            AS+ G   GTA+G +P+ARVA YK+CW    GGCF +DI    D AI D V V+S+S+G
Sbjct: 247 GASLLGYASGTARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLG 306

Query: 306 GSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQA 365
           G  ++Y+ D +AI AF A+++GI V CSAGN+GP + + SN APWI TVGA TLDR F A
Sbjct: 307 GGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPA 366

Query: 366 PVELKNGQRFQGSSL--STALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKG 425
              L NG+ F G SL    ALP DKL P I    A    ++A +  LC   TL   K KG
Sbjct: 367 LAILGNGKNFTGVSLFKGEALP-DKLLPFIYAGNA----SNATNGNLCMTGTLIPEKVKG 426

Query: 426 KILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVL 485
           KI+ C RG NARV KG+    AG  GMIL N   +G E +AD HLLPA+ V    G  + 
Sbjct: 427 KIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 486

Query: 486 KYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYT 545
            Y+ +  NP   +      +  KP+PV+AAFSSRGPN +TP I+KPD+ APGVN++AA+T
Sbjct: 487 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 546

Query: 546 EAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIR 605
            A  PTG   D+R   F  +SGTSMSCPHV+GLA LL+S+HP WSP+AI+SA+MT+A   
Sbjct: 547 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 606

Query: 606 DNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKK 665
               KP+LD  T +  P+TPF +G+GH+ P  A +PGL+YDLT +DYL FLCA+ Y   +
Sbjct: 607 YKDGKPLLDIATGK--PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQ 666

Query: 666 IRAFSDGPYKCPPSA--SLLNFNYPSIGVQ-NMTGSVTVTRRLKNVGTPGVYRARV-RQP 725
           IR+ S   Y C PS   S+ + NYPS  V  +  G+   TR + +VG  G Y  +V  + 
Sbjct: 667 IRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSET 726

Query: 726 EGVRVSVEPRFLKFDKVGEEKSFKL--TIAGVVPAKRVVDGTLIWTDGEHFVRSPIVIS 776
            GV++SVEP  L F +  E+KS+ +  T+    P+     G++ W+DG+H V SP+ IS
Sbjct: 727 TGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Moc03g23150 vs. ExPASy Swiss-Prot
Match: O49607 (Subtilisin-like protease SBT1.6 OS=Arabidopsis thaliana OX=3702 GN=SBT1.6 PE=2 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.1e-155
Identity = 315/733 (42.97%), Postives = 443/733 (60.44%), Query Frame = 0

Query: 66  SSEKAKDA-IFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMR 125
           S+E A+++ I + Y    +GF+A++  +EA  L  HP V AV  ++ ++LHTT S  F+ 
Sbjct: 49  STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108

Query: 126 LEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDD- 185
           L+   G+      W  +++G D II   DTG+WPE +SF +  + G +P +W+G C    
Sbjct: 109 LQNQKGL------WSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGA 168

Query: 186 --SPDRVPCNRKLIGAKYFNKG-FIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 245
             SP    CNRK+IGA++F KG   A +  +N + +      S RD +GHG+HT STA G
Sbjct: 169 RFSPRN--CNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAG 228

Query: 246 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 305
                AS+ G   G AKG +PKAR+AAYK+CW  +D GC D+DI   FD A+ DGV+VIS
Sbjct: 229 RHAFKASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVIS 288

Query: 306 LSVGGS---PANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGAST 365
           +S+GG     + Y+ D IAI ++ A  KGI V  SAGN GP+  + +N APW+ TVGAST
Sbjct: 289 ISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGAST 348

Query: 366 LDRQFQAPVELKNGQRFQGSSLSTALP-EDKLYPLITGAQAKAENASAADAMLCKPETLD 425
           +DR F A   L +G R +G SL   +P   +++P++   ++   +AS     LC   TLD
Sbjct: 349 IDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLD 408

Query: 426 HSKAKGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYN 485
             + +GKI+ C RG + RV KG     AG  GMIL N   +G   + D HL+PA  V  N
Sbjct: 409 PKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSN 468

Query: 486 DGQAVLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVN 545
           +G  +  Y  S  NP+  +      +  KPAPV+A+FS RGPN ++PEI+KPD+ APGVN
Sbjct: 469 EGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVN 528

Query: 546 VIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIM 605
           ++AA+T+AV PTG P D R   F  +SGTSM+CPHV+G A LL+S HP+WSP+ I+SA+M
Sbjct: 529 ILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMM 588

Query: 606 TSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAI 665
           T+  + DN+ + ++D  T +   ATP+ YGSGH+    A++PGLVYD+T DDY+ FLC+I
Sbjct: 589 TTTNLVDNSNRSLIDESTGK--SATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSI 648

Query: 666 GYDEKKIRAFSDGPYKCPPS--ASLLNFNYPSIGV---QNMTG--SVTVTRRLKNVG-TP 725
           GY  K I+  +  P +CP +   S  N NYPSI      N  G  S TV R   NVG   
Sbjct: 649 GYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAE 708

Query: 726 GVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA-----GVVPAKRVVDGTLIWTD 776
            VYRAR+  P GV V+V+P  L F    + +S+ +T+       V+     V G++ W D
Sbjct: 709 AVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD 759

BLAST of Moc03g23150 vs. ExPASy TrEMBL
Match: A0A6J1CN43 (subtilisin-like protease SBT5.4 OS=Momordica charantia OX=3673 GN=LOC111012645 PE=3 SV=1)

HSP 1 Score: 1559.3 bits (4036), Expect = 0.0e+00
Identity = 779/779 (100.00%), Postives = 779/779 (100.00%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60
           MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL
Sbjct: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60

Query: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120
           GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW
Sbjct: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120

Query: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180
           NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC
Sbjct: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180

Query: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240
           TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG
Sbjct: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240

Query: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300
           SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS
Sbjct: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300

Query: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360
           LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR
Sbjct: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360

Query: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420
           QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA
Sbjct: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420

Query: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480
           KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA
Sbjct: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480

Query: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540
           VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA
Sbjct: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540

Query: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600
           YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR
Sbjct: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600

Query: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660
           IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE
Sbjct: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660

Query: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720
           KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG
Sbjct: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720

Query: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF
Sbjct: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 779

BLAST of Moc03g23150 vs. ExPASy TrEMBL
Match: A0A1S3CLB7 (subtilisin-like protease SBT5.4 OS=Cucumis melo OX=3656 GN=LOC103502236 PE=3 SV=1)

HSP 1 Score: 1257.3 bits (3252), Expect = 0.0e+00
Identity = 612/779 (78.56%), Postives = 694/779 (89.09%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLL 60
           MK+ NSIL +FS L    L+ISPAIAAKKSY+V+LGSHSHGLEV + DL+RV DSHHKLL
Sbjct: 6   MKIYNSILILFSLL----LVISPAIAAKKSYIVLLGSHSHGLEVRDEDLERVADSHHKLL 65

Query: 61  GSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSW 120
           GS +GS EKA+DAIFYSYK+NINGFAAI++EEEA +LAKHPEVAAVL NK KKLHTTHSW
Sbjct: 66  GSIIGSDEKARDAIFYSYKRNINGFAAIMDEEEATQLAKHPEVAAVLLNKAKKLHTTHSW 125

Query: 121 NFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGC 180
            FM LEK NGV+PP+S W RA  G+D II NLDTGVW ESKSFGE+GIVG+VP+KWKGGC
Sbjct: 126 EFMHLEK-NGVIPPSSAWRRAKSGKDVIIGNLDTGVWGESKSFGEHGIVGAVPSKWKGGC 185

Query: 181 TDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 240
           TD +PD V CNRKLIGAKYFNKGF+AYL + N +A   S+ NSTRDY+GHGSHTLSTAGG
Sbjct: 186 TDKTPDGVSCNRKLIGAKYFNKGFLAYLNSQNLTA---SVINSTRDYDGHGSHTLSTAGG 245

Query: 241 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 300
           S+V  ASVFG G+GTAKGGSPKARVA+YK+CWP +DGGCF+ADI E FDHAIHD V+V+S
Sbjct: 246 SYVSGASVFGLGVGTAKGGSPKARVASYKVCWPLEDGGCFEADIAEAFDHAIHDRVDVLS 305

Query: 301 LSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDR 360
           LS+GG PA+Y+DD IAIAAFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGASTLDR
Sbjct: 306 LSLGGEPADYYDDGIAIAAFHAVKKGIPVVCSAGNSGPAAQTVSNTAPWILTVGASTLDR 365

Query: 361 QFQAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKA 420
           +FQAPVEL+NG  + GSSLS  L  DKLYPLITGA+AKA+NA+A  AMLCKP+TLDHSK 
Sbjct: 366 EFQAPVELQNGHSYMGSSLSKGLKGDKLYPLITGAEAKAKNATAEVAMLCKPKTLDHSKV 425

Query: 421 KGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQA 480
           KGKIL CLRGD ARVDKGEQAALAGA GMILCND+LSGFETIADPH+LPASH+NYNDGQA
Sbjct: 426 KGKILVCLRGDTARVDKGEQAALAGAVGMILCNDKLSGFETIADPHVLPASHINYNDGQA 485

Query: 481 VLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAA 540
           V  YI+ST+NPMG LIPP+AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA
Sbjct: 486 VFSYIKSTKNPMGSLIPPSAKVNTKPAPSMAAFSSRGPNLISPEIIKPDVTAPGVNIIAA 545

Query: 541 YTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSAR 600
           ++EAVSPTGEPFDNRT PFITMSGTSMSCPHV+GL GLLR+LHP+WSPSAIKSAIMTSAR
Sbjct: 546 FSEAVSPTGEPFDNRTVPFITMSGTSMSCPHVSGLVGLLRTLHPHWSPSAIKSAIMTSAR 605

Query: 601 IRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDE 660
           IRDNT KPMLDGG+ +LAPATPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+E
Sbjct: 606 IRDNTKKPMLDGGSPDLAPATPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNE 665

Query: 661 KKIRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEG 720
           K I+AFSDGP+KCP SAS+LNFNYPSIGVQN+TGSVT+TR+LKNV TPGVY+ARV  P G
Sbjct: 666 KTIQAFSDGPFKCPASASILNFNYPSIGVQNLTGSVTLTRKLKNVSTPGVYKARVMHPNG 725

Query: 721 VRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           V+V V+P+ LKF++VGEEK F+L I G VP  +VVDG LIWTDG+HFVRSPIV+SSGLF
Sbjct: 726 VKVLVKPKVLKFERVGEEKRFELIITGDVPENQVVDGVLIWTDGKHFVRSPIVVSSGLF 776

BLAST of Moc03g23150 vs. ExPASy TrEMBL
Match: A0A0A0LVY8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G171040 PE=3 SV=1)

HSP 1 Score: 1242.3 bits (3213), Expect = 0.0e+00
Identity = 599/766 (78.20%), Postives = 683/766 (89.16%), Query Frame = 0

Query: 14  LLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDA 73
           ++LF L++       +SY+V+LGSHSHGLEV++ DL+RV DSHHKLLGS  GS EKA++A
Sbjct: 1   MVLFCLML-----GFQSYIVLLGSHSHGLEVTDEDLKRVADSHHKLLGSIFGSDEKARNA 60

Query: 74  IFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNGVVP 133
           IFYSYKKNINGFAAI++EEEAA+LAKHPEVAAVL N+ KKLHTTHSW FM LEK NGV+P
Sbjct: 61  IFYSYKKNINGFAAIMDEEEAAQLAKHPEVAAVLPNRAKKLHTTHSWEFMHLEK-NGVIP 120

Query: 134 PTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRK 193
           P+S W RA  G+D IIANLDTGVWPESKSFGE+GIVG VP+KWKGGCTD + DRVPCNRK
Sbjct: 121 PSSAWRRAKSGKDVIIANLDTGVWPESKSFGEHGIVGPVPSKWKGGCTDKTLDRVPCNRK 180

Query: 194 LIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFGSGL 253
           LIGAKYFNKGF+AYLK+ N +A    + NSTRDY+GHGSHTLSTAGGS+V  ASVFG G+
Sbjct: 181 LIGAKYFNKGFLAYLKSENLTA---LVINSTRDYDGHGSHTLSTAGGSYVSGASVFGLGV 240

Query: 254 GTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDD 313
           GTAKGGSPKARVAAYK+CWP +DGGCFDADI + FDHAIHD V+V+SLS+GG PA+Y+DD
Sbjct: 241 GTAKGGSPKARVAAYKVCWPLEDGGCFDADIAQAFDHAIHDRVDVLSLSLGGEPADYYDD 300

Query: 314 SIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQR 373
            IAI+AFHAVKKGIPVVCSAGNSGP A T SNTAPWILTVGAST+DR+FQAPVEL+NG R
Sbjct: 301 GIAISAFHAVKKGIPVVCSAGNSGPGAQTVSNTAPWILTVGASTMDREFQAPVELQNGHR 360

Query: 374 FQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNA 433
           + GSSLS  L  DKLYPLITGA+AKA+NA+A +A LCKP+TLDHSK KGKIL CLRGD A
Sbjct: 361 YMGSSLSKGLKGDKLYPLITGAEAKAKNATAEEARLCKPKTLDHSKVKGKILVCLRGDTA 420

Query: 434 RVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMG 493
           RVDKGEQAALAGA GMILCNDELSGFETIADPH+LPASH+NYNDGQAV  YI++T+NPMG
Sbjct: 421 RVDKGEQAALAGAVGMILCNDELSGFETIADPHVLPASHINYNDGQAVFSYIKTTKNPMG 480

Query: 494 YLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFD 553
           YLIPP AK+NTKPAP MAAFSSRGPN+++PEIIKPDVTAPGVN+IAA++EAVSPTGEPFD
Sbjct: 481 YLIPPTAKVNTKPAPTMAAFSSRGPNLISPEIIKPDVTAPGVNIIAAFSEAVSPTGEPFD 540

Query: 554 NRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGG 613
           NRT PFITMSGTSMSCPHV+GL GLLR+LHP WSPSAIKSAIMTSARIRDNT KPMLDGG
Sbjct: 541 NRTVPFITMSGTSMSCPHVSGLVGLLRTLHPQWSPSAIKSAIMTSARIRDNTKKPMLDGG 600

Query: 614 TDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKC 673
           + +LAP+TPF+YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY+EK I+AFSDGP+KC
Sbjct: 601 SPDLAPSTPFAYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYNEKTIQAFSDGPFKC 660

Query: 674 PPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFD 733
           P SAS+LN NYPSIGVQN+TGSVTVTR+LKNV TPGVY+ RVR P GV+V V+P+ LKF+
Sbjct: 661 PASASILNLNYPSIGVQNLTGSVTVTRKLKNVSTPGVYKGRVRHPNGVKVLVKPKVLKFE 720

Query: 734 KVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           +VGEEKSF+LTI G VP  +VVDG LIWTDG+HFVRSPIV+SS LF
Sbjct: 721 RVGEEKSFELTITGDVPEDQVVDGVLIWTDGKHFVRSPIVVSSSLF 757

BLAST of Moc03g23150 vs. ExPASy TrEMBL
Match: A0A5D3CZ66 (Subtilisin-like protease SBT5.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold477G00570 PE=3 SV=1)

HSP 1 Score: 1190.6 bits (3079), Expect = 0.0e+00
Identity = 574/750 (76.53%), Postives = 653/750 (87.07%), Query Frame = 0

Query: 30   SYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLGSSEKAKDAIFYSYKKNINGFAAIL 89
            SYVV+LGSHSHGLEV+E D + VVDSHHKLLGSFL S EKAKD+IFYSYKK+INGFAA L
Sbjct: 755  SYVVLLGSHSHGLEVTEKDFESVVDSHHKLLGSFLRSDEKAKDSIFYSYKKHINGFAATL 814

Query: 90   EEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRLEKMNGVVPPTSPWWRANFGQDTII 149
            ++E+A  LA HPEVAAVL NK K L+TTHSW FM LEK NGVVPP+SPW  A FG+D II
Sbjct: 815  DDEDATRLANHPEVAAVLPNKPKDLYTTHSWEFMHLEK-NGVVPPSSPWRMAKFGKDVII 874

Query: 150  ANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSPDRVPCNRKLIGAKYFNKGFIAYLK 209
            ANLDTGVWPESKSFGE+GI G  P+KWKGGCTD SPD VPCN+KLIGAKYFNKG++ YLK
Sbjct: 875  ANLDTGVWPESKSFGEHGIDGPAPSKWKGGCTDKSPDGVPCNKKLIGAKYFNKGYLEYLK 934

Query: 210  ALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPDASVFGSGLGTAKGGSPKARVAAYK 269
            + NS+ DLSSI NSTRDY+GHGSHTLSTA G++V  ASVFGSG+GTAKGGSPKARVAAYK
Sbjct: 935  SENSTVDLSSIINSTRDYDGHGSHTLSTAAGNYVFGASVFGSGIGTAKGGSPKARVAAYK 994

Query: 270  ICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVGGSPANYFDDSIAIAAFHAVKKGIPV 329
            +CWPF+ GGCFDADITE FDHAIHDGV+V+SLS+GG P  Y +DSIAIA+FHAVKKGIPV
Sbjct: 995  VCWPFEQGGCFDADITEAFDHAIHDGVDVLSLSLGGDPIKYSEDSIAIASFHAVKKGIPV 1054

Query: 330  VCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQAPVELKNGQRFQGSSLSTALPEDKLY 389
            VC+ GNSGP   TASNTAPWILTVGASTLDR+F APV L+NG RF GSS S  L   KLY
Sbjct: 1055 VCAVGNSGPTPKTASNTAPWILTVGASTLDREFYAPVVLQNGHRFMGSSHSKGLTGRKLY 1114

Query: 390  PLITGAQAKAENASAADAMLCKPETLDHSKAKGKILACLRGDNARVDKGEQAALAGAAGM 449
            PLITGAQAKA NA+  DAMLCKPETLDHSK KGKIL CLRG+ AR+DKG+QAALAGA GM
Sbjct: 1115 PLITGAQAKAGNANEDDAMLCKPETLDHSKVKGKILVCLRGETARLDKGKQAALAGAVGM 1174

Query: 450  ILCNDELSGFETIADPHLLPASHVNYNDGQAVLKYIQSTQNPMGYLIPPAAKLNTKPAPV 509
            ILCND+LSG   + D HLLPASH+NY DGQ +L YI S +NPMGYLIPP AK+NTKPAP 
Sbjct: 1175 ILCNDKLSGSSIVPDFHLLPASHINYQDGQVLLSYINSARNPMGYLIPPLAKVNTKPAPT 1234

Query: 510  MAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSC 569
            MA FSSRGPN ++PEIIKPDVTAPGVN+IAA++EA+SPT +  DNRT PFITMSGTSMSC
Sbjct: 1235 MAVFSSRGPNTISPEIIKPDVTAPGVNIIAAFSEAISPTRDASDNRTTPFITMSGTSMSC 1294

Query: 570  PHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGH 629
            PHVAGL GLLR+LHP+WSPSAIKSAIMTS+++RDNT+ PM+DGG+ +LAPATPF+YGSGH
Sbjct: 1295 PHVAGLVGLLRNLHPDWSPSAIKSAIMTSSQVRDNTLNPMIDGGSLDLAPATPFAYGSGH 1354

Query: 630  IRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIRAFSDGPYKCPPSASLLNFNYPSIGV 689
            I P GA+DPGLVYDL+P+DYLEFLCA GYDEK IRAFSD P+KCPP++S+LN NYPSIGV
Sbjct: 1355 INPTGAIDPGLVYDLSPNDYLEFLCASGYDEKTIRAFSDEPFKCPPASSVLNLNYPSIGV 1414

Query: 690  QNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVV 749
            QN+ GSV+VTR+LKNVG+PGVYRA++  P GV VSV+PRFLKF++VGEEKSF+LT+AGVV
Sbjct: 1415 QNLKGSVSVTRKLKNVGSPGVYRAQILHPNGVVVSVKPRFLKFERVGEEKSFELTLAGVV 1474

Query: 750  PAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
            P  RV  G LIW+DG H VRSPIV+SSGLF
Sbjct: 1475 PKDRVGYGALIWSDGRHVVRSPIVVSSGLF 1503

BLAST of Moc03g23150 vs. ExPASy TrEMBL
Match: A0A6J1EG66 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE=3 SV=1)

HSP 1 Score: 1190.3 bits (3078), Expect = 0.0e+00
Identity = 580/777 (74.65%), Postives = 659/777 (84.81%), Query Frame = 0

Query: 4   SNSILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSF 63
           +N I  +F     F  LISP IA KKSY+V+LGSHSHGLEVS  DLQR VDSHHKLLGSF
Sbjct: 5   TNFIFMLFG----FFWLISPVIAVKKSYIVLLGSHSHGLEVSAVDLQRAVDSHHKLLGSF 64

Query: 64  LGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFM 123
           LGSS KAKDAIFYSYK +INGFAAILEEEEA +LAKHPEVAAVL NK K+LHTTHSW  M
Sbjct: 65  LGSSGKAKDAIFYSYKNHINGFAAILEEEEATKLAKHPEVAAVLPNKAKELHTTHSWESM 124

Query: 124 RLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDD 183
            LEK N V+P +SPW +A FG+D IIANLD+GVWPESKSFGE GIVG VP++WKGGC D 
Sbjct: 125 HLEK-NDVIPSSSPWRKARFGEDVIIANLDSGVWPESKSFGEQGIVGGVPSRWKGGCMDK 184

Query: 184 SPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFV 243
           +PD VPCNRKLIGAKYFN+G +AYLK+ N   +L  I NSTRDY+GHGSHTLSTAGGS+V
Sbjct: 185 TPDGVPCNRKLIGAKYFNQGALAYLKSQNLMEELPLIVNSTRDYKGHGSHTLSTAGGSYV 244

Query: 244 PDASVFGSGLGTAKGGSPKARVAAYKICWPFQD-GGCFDADITEGFDHAIHDGVEVISLS 303
            + SVFGSG+GTAKGGSPKARVAAYK+CWP  + GGCFDAD+ E FDHAIHDGV+V+SLS
Sbjct: 245 SNVSVFGSGIGTAKGGSPKARVAAYKVCWPLPNIGGCFDADVAEAFDHAIHDGVDVLSLS 304

Query: 304 VGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQF 363
           +G  PA Y+DD IAI +FHA+KKGIPVVCSAGNSGP   TASN APWILTVGASTLDR+F
Sbjct: 305 IGSPPAEYYDDIIAIGSFHALKKGIPVVCSAGNSGPSMATASNIAPWILTVGASTLDREF 364

Query: 364 QAPVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKG 423
           QAP+EL NGQRF GSSLS  L   KLYPLITGAQAKA  A A D++LCKPETLDHSK KG
Sbjct: 365 QAPIELGNGQRFTGSSLSNPLAGRKLYPLITGAQAKATTAFAQDSILCKPETLDHSKVKG 424

Query: 424 KILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVL 483
           KIL CLRG ++R+DKG QA LAGA GMILCND LSGFE +AD H+LP SH+NYNDGQAV 
Sbjct: 425 KILVCLRGGSSRIDKGMQALLAGAVGMILCNDRLSGFEIMADLHVLPTSHINYNDGQAVF 484

Query: 484 KYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYT 543
            YI ST+NPMGYLIPP++K+NTKP+P MAAFSSRGPNIV+PEIIKPDVTAPGVN+IAA++
Sbjct: 485 SYIYSTKNPMGYLIPPSSKVNTKPSPTMAAFSSRGPNIVSPEIIKPDVTAPGVNIIAAFS 544

Query: 544 EAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIR 603
            AVSPTGEPFDNR  P+ITMSGTSMSCPHV+G+ GLL++LHP WSP+AIKSAIMTSAR+ 
Sbjct: 545 GAVSPTGEPFDNRAVPYITMSGTSMSCPHVSGIVGLLKALHPEWSPAAIKSAIMTSARVS 604

Query: 604 DNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKK 663
           DNTM  MLDGG+   APATPF YGSGHIRP GA+DPGLVYDL+P+DYLEFLCA GY EK 
Sbjct: 605 DNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCASGYTEKN 664

Query: 664 IRAFSDGPYKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVR 723
           +R F+DG +KCP S S+LNFNYPSIGVQN+TGSVTV+RRLKNVG PGVYR RV+QPEGV+
Sbjct: 665 LRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRVQQPEGVK 724

Query: 724 VSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVDGTLIWTDGEHFVRSPIVISSGLF 780
           VSV+P  LKF+K+GEEK F+LT+ G V   ++  GTLIWTD +HFVRSPIV+SS  F
Sbjct: 725 VSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIVVSSSFF 776

BLAST of Moc03g23150 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 936.8 bits (2420), Expect = 1.1e-272
Identity = 473/772 (61.27%), Postives = 581/772 (75.26%), Query Frame = 0

Query: 6   SILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLG 65
           S+ ++ S LLL +L  SPA A KKSY+V LGSH+H  ++S A L  V  SH   L SF+G
Sbjct: 17  SLQSLSSLLLLVTLFFSPAFALKKSYIVYLGSHAHLPQISSAHLDGVAHSHRTFLASFVG 76

Query: 66  SSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRL 125
           S E AK+AIFYSYK++INGFAAIL+E EAAE+AKHP+V +V  NKG+KLHTTHSWNFM L
Sbjct: 77  SHENAKEAIFYSYKRHINGFAAILDENEAAEIAKHPDVVSVFPNKGRKLHTTHSWNFMLL 136

Query: 126 EKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSP 185
            K NGVV  +S W +A +G+DTIIANLDTGVWPESKSF + G  G+VP +WKG C  D  
Sbjct: 137 AK-NGVVHKSSLWNKAGYGEDTIIANLDTGVWPESKSFSDEG-YGAVPARWKGRCHKD-- 196

Query: 186 DRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVPD 245
             VPCNRKLIGA+YFNKG++AY   L S+A       + RD++GHGSHTLSTA G+FVP 
Sbjct: 197 --VPCNRKLIGARYFNKGYLAY-TGLPSNASY----ETCRDHDGHGSHTLSTAAGNFVPG 256

Query: 246 ASVFGSGLGTAKGGSPKARVAAYKICWPFQDGG-CFDADITEGFDHAIHDGVEVISLSVG 305
           A+VFG G GTA GGSPKARVAAYK+CWP  DG  CFDADI    + AI DGV+V+S SVG
Sbjct: 257 ANVFGIGNGTASGGSPKARVAAYKVCWPPVDGAECFDADILAAIEAAIEDGVDVLSASVG 316

Query: 306 GSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQA 365
           G   +Y  D IAI +FHAVK G+ VVCSAGNSGP + T SN APW++TVGAS++DR+FQA
Sbjct: 317 GDAGDYMSDGIAIGSFHAVKNGVTVVCSAGNSGPKSGTVSNVAPWVITVGASSMDREFQA 376

Query: 366 PVELKNGQRFQGSSLSTALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKGKI 425
            VELKNGQ F+G+SLS  LPE+K+Y LI+ A A   N +  DA+LCK  +LD  K KGKI
Sbjct: 377 FVELKNGQSFKGTSLSKPLPEEKMYSLISAADANVANGNVTDALLCKKGSLDPKKVKGKI 436

Query: 426 LACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVLKY 485
           L CLRGDNARVDKG QAA AGAAGM+LCND+ SG E I+D H+LPAS ++Y DG+ +  Y
Sbjct: 437 LVCLRGDNARVDKGMQAAAAGAAGMVLCNDKASGNEIISDAHVLPASQIDYKDGETLFSY 496

Query: 486 IQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYTEA 545
           + ST++P GY+  P A LNTKPAP MA+FSSRGPN +TP I+KPD+TAPGVN+IAA+TEA
Sbjct: 497 LSSTKDPKGYIKAPTATLNTKPAPFMASFSSRGPNTITPGILKPDITAPGVNIIAAFTEA 556

Query: 546 VSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIRDN 605
             PT    DNR  PF T SGTSMSCPH++G+ GLL++LHP+WSP+AI+SAIMT++R R+N
Sbjct: 557 TGPTDLDSDNRRTPFNTESGTSMSCPHISGVVGLLKTLHPHWSPAAIRSAIMTTSRTRNN 616

Query: 606 TMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKKIR 665
             KPM+D   +    A PFSYGSGH++P  A  PGLVYDLT  DYL+FLCA+GY+   ++
Sbjct: 617 RRKPMVD---ESFKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCAVGYNNTVVQ 676

Query: 666 AFSDGP-YKCPPSASLLNFNYPSIGVQNMTGSVTVTRRLKNVGTPGVYRARVRQPEGVRV 725
            F++ P Y C   A+LL+FNYPSI V N+TGS+TVTR+LKNVG P  Y AR R+P GVRV
Sbjct: 677 LFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNARFREPLGVRV 736

Query: 726 SVEPRFLKFDKVGEEKSFKLTIAGV-VPAKRVVDGTLIWTDGEHFVRSPIVI 775
           SVEP+ L F+K GE K F++T+  + V     V G L WTD  H+VRSPIV+
Sbjct: 737 SVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIVV 774

BLAST of Moc03g23150 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 818.9 bits (2114), Expect = 3.4e-237
Identity = 434/784 (55.36%), Postives = 554/784 (70.66%), Query Frame = 0

Query: 1   MKLSNSILAIFSSLLLFSLLISPAIAAKK---SYVVILGSHSHGLEVSEADLQRVVDSHH 60
           MKL+++    F  LLL   + S  I A K   SYVV  G+HSH  E++E  + RV ++H+
Sbjct: 1   MKLTHNF--SFLLLLLLVHMSSKHILASKDSSSYVVYFGAHSHVGEITEDAMDRVKETHY 60

Query: 61  KLLGSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTT 120
             LGSF GS E+A DAIFYSY K+INGFAA L+ + A E++KHPEV +V  NK  KLHTT
Sbjct: 61  DFLGSFTGSRERATDAIFYSYTKHINGFAAHLDHDLAYEISKHPEVVSVFPNKALKLHTT 120

Query: 121 HSWNFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWK 180
            SW+F+ LE  N  VP +S W +A FG+DTIIANLDTGVWPESKSF + G+ G +P++WK
Sbjct: 121 RSWDFLGLEH-NSYVPSSSIWRKARFGEDTIIANLDTGVWPESKSFRDEGL-GPIPSRWK 180

Query: 181 GGCTDDSPDRVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLST 240
           G C +       CNRKLIGA+YFNKG+ A +  LNSS D      S RD +GHGSHTLST
Sbjct: 181 GICQNQKDATFHCNRKLIGARYFNKGYAAAVGHLNSSFD------SPRDLDGHGSHTLST 240

Query: 241 AGGSFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGG-CFDADITEGFDHAIHDGV 300
           A G FVP  S+FG G GTAKGGSP+ARVAAYK+CWP   G  C+DAD+   FD AIHDG 
Sbjct: 241 AAGDFVPGVSIFGQGNGTAKGGSPRARVAAYKVCWPPVKGNECYDADVLAAFDAAIHDGA 300

Query: 301 EVISLSVGGSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGAS 360
           +VIS+S+GG P ++F+DS+AI +FHA KK I VVCSAGNSGP   T SN APW +TVGAS
Sbjct: 301 DVISVSLGGEPTSFFNDSVAIGSFHAAKKRIVVVCSAGNSGPADSTVSNVAPWQITVGAS 360

Query: 361 TLDRQFQAPVELKNGQRFQGSSL-STALPEDKLYPLITGAQAKAENASAADAMLCKPETL 420
           T+DR+F + + L NG+ ++G SL STALP  K YP++    AKA+NASA DA LCK  +L
Sbjct: 361 TMDREFASNLVLGNGKHYKGQSLSSTALPHAKFYPIMASVNAKAKNASALDAQLCKLGSL 420

Query: 421 DHSKAKGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNY 480
           D  K KGKIL CLRG N RV+KG   AL G  GM+L N  ++G + +ADPH+LPA+ +  
Sbjct: 421 DPIKTKGKILVCLRGQNGRVEKGRAVALGGGIGMVLENTYVTGNDLLADPHVLPATQLTS 480

Query: 481 NDGQAVLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGV 540
            D  AV +YI  T+ P+ ++ P    L  KPAPVMA+FSS+GP+IV P+I+KPD+TAPGV
Sbjct: 481 KDSFAVSRYISQTKKPIAHITPSRTDLGLKPAPVMASFSSKGPSIVAPQILKPDITAPGV 540

Query: 541 NVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAI 600
           +VIAAYT AVSPT E FD R   F  +SGTSMSCPH++G+AGLL++ +P+WSP+AI+SAI
Sbjct: 541 SVIAAYTGAVSPTNEQFDPRRLLFNAISGTSMSCPHISGIAGLLKTRYPSWSPAAIRSAI 600

Query: 601 MTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCA 660
           MT+A I D+   P+ +        ATPFS+G+GH++P  AV+PGLVYDL   DYL FLC+
Sbjct: 601 MTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNFLCS 660

Query: 661 IGYDEKKIRAFSDGPYKC-PPSASLLNFNYPSIGVQNMTGS-VTVTRRLKNVGTPGVYRA 720
           +GY+  +I  FS   + C  P  SL+N NYPSI V N+T S VTV+R +KNVG P +Y  
Sbjct: 661 LGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYTV 720

Query: 721 RVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA---GVVPAKRVVDGTLIWTDGEHFVRS 775
           +V  P+GV V+V+P  L F KVGE+K+FK+ +    G V AK  V G L+W+D +H VRS
Sbjct: 721 KVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNV-AKGYVFGELVWSDKKHRVRS 770

BLAST of Moc03g23150 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 575.5 bits (1482), Expect = 6.6e-164
Identity = 336/779 (43.13%), Postives = 463/779 (59.44%), Query Frame = 0

Query: 6   SILAIFSSLLLFSLLISPAIAAKKSYVVILGSHSHGLEVSEADLQRVVDSHHKLLGSFLG 65
           S  A F  L L    +S + + + +Y+V          ++++ +    D H     S L 
Sbjct: 7   SSTAFFLLLCLGFCHVSSSSSDQGTYIV---------HMAKSQMPSSFDLHSNWYDSSLR 66

Query: 66  SSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMRL 125
           S   + + + Y+Y+  I+GF+  L +EEA  L   P V +VL     +LHTT +  F+ L
Sbjct: 67  SISDSAE-LLYTYENAIHGFSTRLTQEEADSLMTQPGVISVLPEHRYELHTTRTPLFLGL 126

Query: 126 EKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDDSP 185
           ++    + P      A    D ++  LDTGVWPESKS+ + G  G +P+ WKGGC   + 
Sbjct: 127 DEHTADLFP-----EAGSYSDVVVGVLDTGVWPESKSYSDEGF-GPIPSSWKGGCEAGTN 186

Query: 186 DRVP-CNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGGSFVP 245
                CNRKLIGA++F +G+ + +  ++ S +      S RD +GHG+HT STA GS V 
Sbjct: 187 FTASLCNRKLIGARFFARGYESTMGPIDESKE----SRSPRDDDGHGTHTSSTAAGSVVE 246

Query: 246 DASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVISLSVG 305
            AS+ G   GTA+G +P+ARVA YK+CW    GGCF +DI    D AI D V V+S+S+G
Sbjct: 247 GASLLGYASGTARGMAPRARVAVYKVCWL---GGCFSSDILAAIDKAIADNVNVLSMSLG 306

Query: 306 GSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGASTLDRQFQA 365
           G  ++Y+ D +AI AF A+++GI V CSAGN+GP + + SN APWI TVGA TLDR F A
Sbjct: 307 GGMSDYYRDGVAIGAFAAMERGILVSCSAGNAGPSSSSLSNVAPWITTVGAGTLDRDFPA 366

Query: 366 PVELKNGQRFQGSSL--STALPEDKLYPLITGAQAKAENASAADAMLCKPETLDHSKAKG 425
              L NG+ F G SL    ALP DKL P I    A    ++A +  LC   TL   K KG
Sbjct: 367 LAILGNGKNFTGVSLFKGEALP-DKLLPFIYAGNA----SNATNGNLCMTGTLIPEKVKG 426

Query: 426 KILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYNDGQAVL 485
           KI+ C RG NARV KG+    AG  GMIL N   +G E +AD HLLPA+ V    G  + 
Sbjct: 427 KIVMCDRGINARVQKGDVVKAAGGVGMILANTAANGEELVADAHLLPATTVGEKAGDIIR 486

Query: 486 KYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVNVIAAYT 545
            Y+ +  NP   +      +  KP+PV+AAFSSRGPN +TP I+KPD+ APGVN++AA+T
Sbjct: 487 HYVTTDPNPTASISILGTVVGVKPSPVVAAFSSRGPNSITPNILKPDLIAPGVNILAAWT 546

Query: 546 EAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIMTSARIR 605
            A  PTG   D+R   F  +SGTSMSCPHV+GLA LL+S+HP WSP+AI+SA+MT+A   
Sbjct: 547 GAAGPTGLASDSRRVEFNIISGTSMSCPHVSGLAALLKSVHPEWSPAAIRSALMTTAYKT 606

Query: 606 DNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAIGYDEKK 665
               KP+LD  T +  P+TPF +G+GH+ P  A +PGL+YDLT +DYL FLCA+ Y   +
Sbjct: 607 YKDGKPLLDIATGK--PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQ 666

Query: 666 IRAFSDGPYKCPPSA--SLLNFNYPSIGVQ-NMTGSVTVTRRLKNVGTPGVYRARV-RQP 725
           IR+ S   Y C PS   S+ + NYPS  V  +  G+   TR + +VG  G Y  +V  + 
Sbjct: 667 IRSVSRRNYTCDPSKSYSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSET 726

Query: 726 EGVRVSVEPRFLKFDKVGEEKSFKL--TIAGVVPAKRVVDGTLIWTDGEHFVRSPIVIS 776
            GV++SVEP  L F +  E+KS+ +  T+    P+     G++ W+DG+H V SP+ IS
Sbjct: 727 TGVKISVEPAVLNFKEANEKKSYTVTFTVDSSKPSGSNSFGSIEWSDGKHVVGSPVAIS 755

BLAST of Moc03g23150 vs. TAIR 10
Match: AT4G34980.1 (subtilisin-like serine protease 2 )

HSP 1 Score: 552.0 bits (1421), Expect = 7.8e-157
Identity = 315/733 (42.97%), Postives = 443/733 (60.44%), Query Frame = 0

Query: 66  SSEKAKDA-IFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHSWNFMR 125
           S+E A+++ I + Y    +GF+A++  +EA  L  HP V AV  ++ ++LHTT S  F+ 
Sbjct: 49  STEFAEESRIVHVYHTVFHGFSAVVTPDEADNLRNHPAVLAVFEDRRRELHTTRSPQFLG 108

Query: 126 LEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGGCTDD- 185
           L+   G+      W  +++G D II   DTG+WPE +SF +  + G +P +W+G C    
Sbjct: 109 LQNQKGL------WSESDYGSDVIIGVFDTGIWPERRSFSDLNL-GPIPKRWRGVCESGA 168

Query: 186 --SPDRVPCNRKLIGAKYFNKG-FIAYLKALNSSADLSSIKNSTRDYEGHGSHTLSTAGG 245
             SP    CNRK+IGA++F KG   A +  +N + +      S RD +GHG+HT STA G
Sbjct: 169 RFSPRN--CNRKIIGARFFAKGQQAAVIGGINKTVEFL----SPRDADGHGTHTSSTAAG 228

Query: 246 SFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVEVIS 305
                AS+ G   G AKG +PKAR+AAYK+CW  +D GC D+DI   FD A+ DGV+VIS
Sbjct: 229 RHAFKASMSGYASGVAKGVAPKARIAAYKVCW--KDSGCLDSDILAAFDAAVRDGVDVIS 288

Query: 306 LSVGGS---PANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGAST 365
           +S+GG     + Y+ D IAI ++ A  KGI V  SAGN GP+  + +N APW+ TVGAST
Sbjct: 289 ISIGGGDGITSPYYLDPIAIGSYGAASKGIFVSSSAGNEGPNGMSVTNLAPWVTTVGAST 348

Query: 366 LDRQFQAPVELKNGQRFQGSSLSTALP-EDKLYPLITGAQAKAENASAADAMLCKPETLD 425
           +DR F A   L +G R +G SL   +P   +++P++   ++   +AS     LC   TLD
Sbjct: 349 IDRNFPADAILGDGHRLRGVSLYAGVPLNGRMFPVVYPGKSGMSSAS-----LCMENTLD 408

Query: 426 HSKAKGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHVNYN 485
             + +GKI+ C RG + RV KG     AG  GMIL N   +G   + D HL+PA  V  N
Sbjct: 409 PKQVRGKIVICDRGSSPRVAKGLVVKKAGGVGMILANGASNGEGLVGDAHLIPACAVGSN 468

Query: 486 DGQAVLKYIQSTQNPMGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIKPDVTAPGVN 545
           +G  +  Y  S  NP+  +      +  KPAPV+A+FS RGPN ++PEI+KPD+ APGVN
Sbjct: 469 EGDRIKAYASSHPNPIASIDFRGTIVGIKPAPVIASFSGRGPNGLSPEILKPDLIAPGVN 528

Query: 546 VIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWSPSAIKSAIM 605
           ++AA+T+AV PTG P D R   F  +SGTSM+CPHV+G A LL+S HP+WSP+ I+SA+M
Sbjct: 529 ILAAWTDAVGPTGLPSDPRKTEFNILSGTSMACPHVSGAAALLKSAHPDWSPAVIRSAMM 588

Query: 606 TSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPDDYLEFLCAI 665
           T+  + DN+ + ++D  T +   ATP+ YGSGH+    A++PGLVYD+T DDY+ FLC+I
Sbjct: 589 TTTNLVDNSNRSLIDESTGK--SATPYDYGSGHLNLGRAMNPGLVYDITNDDYITFLCSI 648

Query: 666 GYDEKKIRAFSDGPYKCPPS--ASLLNFNYPSIGV---QNMTG--SVTVTRRLKNVG-TP 725
           GY  K I+  +  P +CP +   S  N NYPSI      N  G  S TV R   NVG   
Sbjct: 649 GYGPKTIQVITRTPVRCPTTRKPSPGNLNYPSITAVFPTNRRGLVSKTVIRTATNVGQAE 708

Query: 726 GVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIA-----GVVPAKRVVDGTLIWTD 776
            VYRAR+  P GV V+V+P  L F    + +S+ +T+       V+     V G++ W D
Sbjct: 709 AVYRARIESPRGVTVTVKPPRLVFTSAVKRRSYAVTVTVNTRNVVLGETGAVFGSVTWFD 759

BLAST of Moc03g23150 vs. TAIR 10
Match: AT3G14067.1 (Subtilase family protein )

HSP 1 Score: 545.4 bits (1404), Expect = 7.3e-155
Identity = 344/805 (42.73%), Postives = 468/805 (58.14%), Query Frame = 0

Query: 2   KLS-NSILAIFSSLLLFSLLISPAIAAKKSYVV-ILGSHSHGLEVSEADLQRVVDSHHKL 61
           KLS +SI  +F  LL F    S +    +SY+V +  SH   L  S  +       H  L
Sbjct: 3   KLSLSSIFFVFPLLLCFFSPSSSSSDGLESYIVHVQRSHKPSLFSSHNNW------HVSL 62

Query: 62  LGSFLGSSEKAKDAIFYSYKKNINGFAAILEEEEAAELAKHPEVAAVLANKGKKLHTTHS 121
           L S   S + A   + YSY + ++GF+A L   + A L +HP V +V+ ++ +++HTTH+
Sbjct: 63  LRSLPSSPQPA--TLLYSYSRAVHGFSARLSPIQTAALRRHPSVISVIPDQAREIHTTHT 122

Query: 122 WNFMRLEKMNGVVPPTSPWWRANFGQDTIIANLDTGVWPESKSFGENGIVGSVPTKWKGG 181
             F+   + +G+      W  +N+G+D I+  LDTG+WPE  SF ++G+ G +P+ WKG 
Sbjct: 123 PAFLGFSQNSGL------WSNSNYGEDVIVGVLDTGIWPEHPSFSDSGL-GPIPSTWKGE 182

Query: 182 CTDDSPD--RVPCNRKLIGAKYFNKGFIAYLKALNSSADLSSIKNSTRDYEGHGSHTLST 241
           C +  PD     CNRKLIGA+ F +G++         A   S   S RD EGHG+HT ST
Sbjct: 183 C-EIGPDFPASSCNRKLIGARAFYRGYLTQRNGTKKHAAKES--RSPRDTEGHGTHTAST 242

Query: 242 AGGSFVPDASVFGSGLGTAKGGSPKARVAAYKICWPFQDGGCFDADITEGFDHAIHDGVE 301
           A GS V +AS++    GTA G + KAR+AAYKICW    GGC+D+DI    D A+ DGV 
Sbjct: 243 AAGSVVANASLYQYARGTATGMASKARIAAYKICW---TGGCYDSDILAAMDQAVADGVH 302

Query: 302 VISLSVG--GSPANYFDDSIAIAAFHAVKKGIPVVCSAGNSGPDAFTASNTAPWILTVGA 361
           VISLSVG  GS   Y  DSIAI AF A + GI V CSAGNSGP+  TA+N APWILTVGA
Sbjct: 303 VISLSVGASGSAPEYHTDSIAIGAFGATRHGIVVSCSAGNSGPNPETATNIAPWILTVGA 362

Query: 362 STLDRQFQAPVELKNGQRFQGSSL--STALPEDKLYPLITGAQAKAENASAADAMLCKPE 421
           ST+DR+F A     +G+ F G+SL    +LP+ +L  + +G            + LC P 
Sbjct: 363 STVDREFAANAITGDGKVFTGTSLYAGESLPDSQLSLVYSG---------DCGSRLCYPG 422

Query: 422 TLDHSKAKGKILACLRGDNARVDKGEQAALAGAAGMILCNDELSGFETIADPHLLPASHV 481
            L+ S  +GKI+ C RG NARV+KG    LAG AGMIL N   SG E  AD HL+PA+ V
Sbjct: 423 KLNSSLVEGKIVLCDRGGNARVEKGSAVKLAGGAGMILANTAESGEELTADSHLVPATMV 482

Query: 482 NYNDGQAVLKYIQSTQNP------MGYLIPPAAKLNTKPAPVMAAFSSRGPNIVTPEIIK 541
               G  +  YI+++ +P      +G LI P     + P+P +AAFSSRGPN +TP I+K
Sbjct: 483 GAKAGDQIRDYIKTSDSPTAKISFLGTLIGP-----SPPSPRVAAFSSRGPNHLTPVILK 542

Query: 542 PDVTAPGVNVIAAYTEAVSPTGEPFDNRTAPFITMSGTSMSCPHVAGLAGLLRSLHPNWS 601
           PDV APGVN++A +T  V PT    D R   F  +SGTSMSCPHV+GLA LLR  HP+WS
Sbjct: 543 PDVIAPGVNILAGWTGMVGPTDLDIDPRRVQFNIISGTSMSCPHVSGLAALLRKAHPDWS 602

Query: 602 PSAIKSAIMTSARIRDNTMKPMLDGGTDELAPATPFSYGSGHIRPIGAVDPGLVYDLTPD 661
           P+AIKSA++T+A   +N+ +P+ D  T +   +  F +G+GH+ P  A++PGLVYD+   
Sbjct: 603 PAAIKSALVTTAYDVENSGEPIEDLATGK--SSNSFIHGAGHVDPNKALNPGLVYDIEVK 662

Query: 662 DYLEFLCAIGYDEKKIRAFSDGP--YKCPPSASLL---NFNYPSIGVQ-NMTGSVTVTRR 721
           +Y+ FLCA+GY+   I  F   P  Y    ++ L    + NYPS  V    TG V   +R
Sbjct: 663 EYVAFLCAVGYEFPGILVFLQDPTLYDACETSKLRTAGDLNYPSFSVVFASTGEVVKYKR 722

Query: 722 -LKNVGT--PGVYRARVRQPEGVRVSVEPRFLKFDKVGEEKSFKLTIAGVVPAKRVVD-- 778
            +KNVG+    VY   V+ P  V + V P  L F K      +++T   VV    V    
Sbjct: 723 VVKNVGSNVDAVYEVGVKSPANVEIDVSPSKLAFSKEKSVLEYEVTFKSVVLGGGVGSVP 770