Moc03g22510 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g22510
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr3: 15873938 .. 15874381 (+)
RNA-Seq Expression	Moc03g22510
Synteny	Moc03g22510

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGACGATGAAAATCTTAATCCTACTGCGACTATGTCTTCATCGATGAATCAGCCGCAGATCTCACTCGAGCACTACATCAATCCTTACTTCCTTCATCATTTTGATAACACGACTCTGGTTCTTGTATCAAATTTACTTACCGATGAAAATTACACATTGTGGAGTAGGTCAATGCTCATTGCTCTAACTGTGAAGAACAAAGTCGGCTTCATCGATGGTTCCTTATCACGACCTACTGGTGAGCAATTACATTCTTGGATTATCTGCAACAATGTAGTAATTTCTTGGATTCTCAATTCTTTATCGAAAGAAATTTCTGCACGTGTTCTTTTCTTCGATTCTGCATATGAAATCTGGCTTGATCTAAAAGAAAGGTTTCAAAGACAAAATCGCCCTCTTATTTTTCAATTTCGAAGGGATTTGTCAAATTTGGTGTAA

mRNA sequence

ATGGCCGACGATGAAAATCTTAATCCTACTGCGACTATGTCTTCATCGATGAATCAGCCGCAGATCTCACTCGAGCACTACATCAATCCTTACTTCCTTCATCATTTTGATAACACGACTCTGGTTCTTGTATCAAATTTACTTACCGATGAAAATTACACATTGTGGAGTAGGTCAATGCTCATTGCTCTAACTGTGAAGAACAAAGTCGGCTTCATCGATGGTTCCTTATCACGACCTACTGGTGAGCAATTACATTCTTGGATTATCTGCAACAATGTAGTAATTTCTTGGATTCTCAATTCTTTATCGAAAGAAATTTCTGCACGTGTTCTTTTCTTCGATTCTGCATATGAAATCTGGCTTGATCTAAAAGAAAGGTTTCAAAGACAAAATCGCCCTCTTATTTTTCAATTTCGAAGGGATTTGTCAAATTTGGTGTAA

Coding sequence (CDS)

ATGGCCGACGATGAAAATCTTAATCCTACTGCGACTATGTCTTCATCGATGAATCAGCCGCAGATCTCACTCGAGCACTACATCAATCCTTACTTCCTTCATCATTTTGATAACACGACTCTGGTTCTTGTATCAAATTTACTTACCGATGAAAATTACACATTGTGGAGTAGGTCAATGCTCATTGCTCTAACTGTGAAGAACAAAGTCGGCTTCATCGATGGTTCCTTATCACGACCTACTGGTGAGCAATTACATTCTTGGATTATCTGCAACAATGTAGTAATTTCTTGGATTCTCAATTCTTTATCGAAAGAAATTTCTGCACGTGTTCTTTTCTTCGATTCTGCATATGAAATCTGGCTTGATCTAAAAGAAAGGTTTCAAAGACAAAATCGCCCTCTTATTTTTCAATTTCGAAGGGATTTGTCAAATTTGGTGTAA

Protein sequence

MADDENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLIALTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLDLKERFQRQNRPLIFQFRRDLSNLV

Homology

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BLAST of Moc03g22510 vs. NCBI nr
Match: XP_022154919.1 (uncharacterized protein LOC111022065 [Momordica charantia])

HSP 1 Score: 219.2 bits (557), Expect = 2.4e-53
Identity = 109/146 (74.66%), Postives = 125/146 (85.62%), Query Frame = 0


Query: 1   MADDENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSM 60
           M +D++LNPTA     MN   I +E + NPYFLHH DNT+LVLVS+LLTDENYT WSRS+
Sbjct: 1   MEEDDSLNPTAPGFIPMN--PIVVEQHANPYFLHHSDNTSLVLVSDLLTDENYTSWSRSI 60

Query: 61  LIALTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEI 120
           +IALTVKNK+GF+DGS+SRPT  +LHSWIICNNVVISWI NSLSK+ISA VLF DSA+EI
Sbjct: 61  VIALTVKNKIGFVDGSISRPTDGRLHSWIICNNVVISWIFNSLSKKISASVLFSDSAHEI 120

Query: 121 WLDLKERFQRQNRPLIFQFRRDLSNL 147
           WLDLKERFQRQNRP IFQ RR+LSNL
Sbjct: 121 WLDLKERFQRQNRPRIFQLRRELSNL 144

[Close]

BLAST of Moc03g22510 vs. NCBI nr
Match: XP_022152756.1 (uncharacterized protein LOC111020399 [Momordica charantia])

HSP 1 Score: 216.1 bits (549), Expect = 2.0e-52
Identity = 111/147 (75.51%), Postives = 126/147 (85.71%), Query Frame = 0


Query: 1   MADDENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSM 60
           MADD  +N TA+ S+ +    I++E Y NPYFLHH DNT+LVLVS+ LT+ENYT WSRSM
Sbjct: 1   MADD-FINLTASTSTII--ALIAIEQYTNPYFLHHSDNTSLVLVSDPLTNENYTSWSRSM 60

Query: 61  LIALTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEI 120
           LIALTVKNKVGF+DGS+ RPTG+ LHSWIICNNVVISWILNSLSKEISA +LF DSA EI
Sbjct: 61  LIALTVKNKVGFVDGSIVRPTGDLLHSWIICNNVVISWILNSLSKEISASILFSDSAREI 120

Query: 121 WLDLKERFQRQNRPLIFQFRRDLSNLV 148
           WLDLKERF++QNRP IFQ RRDLSNLV
Sbjct: 121 WLDLKERFEKQNRPRIFQLRRDLSNLV 144

[Close]

BLAST of Moc03g22510 vs. NCBI nr
Match: XP_022142327.1 (uncharacterized protein LOC111012468 [Momordica charantia])

HSP 1 Score: 197.6 bits (501), Expect = 7.5e-47
Identity = 92/123 (74.80%), Postives = 111/123 (90.24%), Query Frame = 0


Query: 24  LEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLIALTVKNKVGFIDGSLSRPTGE 83
           LE+Y NPY+LHH DNT+LVLVS+LL + NYT WSRSM+IALTVKNK+GF+DGS+SRP+G 
Sbjct: 36  LENYSNPYYLHHSDNTSLVLVSDLLNENNYTSWSRSMIIALTVKNKIGFVDGSISRPSGA 95

Query: 84  QLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLDLKERFQRQNRPLIFQFRRDL 143
           Q++SW ICNNVVI+W+LNSLSKEISA VLF DSA +IWLDL+ER+QR+NRP IFQ RR+L
Sbjct: 96  QINSWKICNNVVIAWLLNSLSKEISASVLFSDSARDIWLDLQERYQRKNRPRIFQLRREL 155

Query: 144 SNL 147
           SNL
Sbjct: 156 SNL 158

[Close]

BLAST of Moc03g22510 vs. NCBI nr
Match: XP_022154608.1 (uncharacterized protein LOC111021831 [Momordica charantia])

HSP 1 Score: 171.0 bits (432), Expect = 7.6e-39
Identity = 83/143 (58.04%), Postives = 112/143 (78.32%), Query Frame = 0


Query: 4   DENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLIA 63
           D++ +P ++ S S       ++  +NPY+LHH DNT LVLV+  LT+ENY+ WSRSMLIA
Sbjct: 21  DDSSSPNSSSSVS------GVDSVLNPYYLHHTDNTELVLVTQPLTEENYSSWSRSMLIA 80

Query: 64  LTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLD 123
           L++KNK+GFIDGS+SRP GE L +WI  N+VVI+WILNS+SKEIS+ +LF +SA +IW+D
Sbjct: 81  LSIKNKLGFIDGSISRPIGELLPAWIHNNHVVIAWILNSVSKEISSSILFSESARDIWID 140

Query: 124 LKERFQRQNRPLIFQFRRDLSNL 147
           LKERF++ N P IFQ +RDL+ L
Sbjct: 141 LKERFEKSNGPRIFQLKRDLAQL 157

[Close]

BLAST of Moc03g22510 vs. NCBI nr
Match: XP_038887186.1 (uncharacterized protein LOC120077373 [Benincasa hispida])

HSP 1 Score: 169.9 bits (429), Expect = 1.7e-38
Identity = 87/144 (60.42%), Postives = 110/144 (76.39%), Query Frame = 0


Query: 8   NPTAT-MSSSMNQPQIS----LEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLI 67
           +PT++  S+SM   + S    LE Y NPYFLH  D+T+LVLVSNLLT+ NY  WS++M I
Sbjct: 6   DPTSSGNSNSMENVRASLSSFLEQYQNPYFLHPSDSTSLVLVSNLLTESNYDSWSQAMKI 65

Query: 68  ALTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWL 127
            LTVKNK+GFI+G + RP+GE L SWII N +V +WILNSLSKEI A + F DSA EIW 
Sbjct: 66  GLTVKNKLGFINGEIPRPSGELLSSWIIYNGIVTTWILNSLSKEIFASINFSDSAQEIWA 125

Query: 128 DLKERFQRQNRPLIFQFRRDLSNL 147
           DL+ER+QR+NRP +FQ RR++SNL
Sbjct: 126 DLQERYQRKNRPRVFQLRREISNL 149

[Close]

BLAST of Moc03g22510 vs. ExPASy TrEMBL
Match: A0A6J1DNP7 (uncharacterized protein LOC111022065 OS=Momordica charantia OX=3673 GN=LOC111022065 PE=4 SV=1)

HSP 1 Score: 219.2 bits (557), Expect = 1.2e-53
Identity = 109/146 (74.66%), Postives = 125/146 (85.62%), Query Frame = 0


Query: 1   MADDENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSM 60
           M +D++LNPTA     MN   I +E + NPYFLHH DNT+LVLVS+LLTDENYT WSRS+
Sbjct: 1   MEEDDSLNPTAPGFIPMN--PIVVEQHANPYFLHHSDNTSLVLVSDLLTDENYTSWSRSI 60

Query: 61  LIALTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEI 120
           +IALTVKNK+GF+DGS+SRPT  +LHSWIICNNVVISWI NSLSK+ISA VLF DSA+EI
Sbjct: 61  VIALTVKNKIGFVDGSISRPTDGRLHSWIICNNVVISWIFNSLSKKISASVLFSDSAHEI 120

Query: 121 WLDLKERFQRQNRPLIFQFRRDLSNL 147
           WLDLKERFQRQNRP IFQ RR+LSNL
Sbjct: 121 WLDLKERFQRQNRPRIFQLRRELSNL 144

[Close]

BLAST of Moc03g22510 vs. ExPASy TrEMBL
Match: A0A6J1DIP8 (uncharacterized protein LOC111020399 OS=Momordica charantia OX=3673 GN=LOC111020399 PE=4 SV=1)

HSP 1 Score: 216.1 bits (549), Expect = 9.9e-53
Identity = 111/147 (75.51%), Postives = 126/147 (85.71%), Query Frame = 0


Query: 1   MADDENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSM 60
           MADD  +N TA+ S+ +    I++E Y NPYFLHH DNT+LVLVS+ LT+ENYT WSRSM
Sbjct: 1   MADD-FINLTASTSTII--ALIAIEQYTNPYFLHHSDNTSLVLVSDPLTNENYTSWSRSM 60

Query: 61  LIALTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEI 120
           LIALTVKNKVGF+DGS+ RPTG+ LHSWIICNNVVISWILNSLSKEISA +LF DSA EI
Sbjct: 61  LIALTVKNKVGFVDGSIVRPTGDLLHSWIICNNVVISWILNSLSKEISASILFSDSAREI 120

Query: 121 WLDLKERFQRQNRPLIFQFRRDLSNLV 148
           WLDLKERF++QNRP IFQ RRDLSNLV
Sbjct: 121 WLDLKERFEKQNRPRIFQLRRDLSNLV 144

[Close]

BLAST of Moc03g22510 vs. ExPASy TrEMBL
Match: A0A6J1CMF8 (uncharacterized protein LOC111012468 OS=Momordica charantia OX=3673 GN=LOC111012468 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.7e-47
Identity = 92/123 (74.80%), Postives = 111/123 (90.24%), Query Frame = 0


Query: 24  LEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLIALTVKNKVGFIDGSLSRPTGE 83
           LE+Y NPY+LHH DNT+LVLVS+LL + NYT WSRSM+IALTVKNK+GF+DGS+SRP+G 
Sbjct: 36  LENYSNPYYLHHSDNTSLVLVSDLLNENNYTSWSRSMIIALTVKNKIGFVDGSISRPSGA 95

Query: 84  QLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLDLKERFQRQNRPLIFQFRRDL 143
           Q++SW ICNNVVI+W+LNSLSKEISA VLF DSA +IWLDL+ER+QR+NRP IFQ RR+L
Sbjct: 96  QINSWKICNNVVIAWLLNSLSKEISASVLFSDSARDIWLDLQERYQRKNRPRIFQLRREL 155

Query: 144 SNL 147
           SNL
Sbjct: 156 SNL 158

[Close]

BLAST of Moc03g22510 vs. ExPASy TrEMBL
Match: A0A6J1DKR8 (uncharacterized protein LOC111021831 OS=Momordica charantia OX=3673 GN=LOC111021831 PE=4 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 3.7e-39
Identity = 83/143 (58.04%), Postives = 112/143 (78.32%), Query Frame = 0


Query: 4   DENLNPTATMSSSMNQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLIA 63
           D++ +P ++ S S       ++  +NPY+LHH DNT LVLV+  LT+ENY+ WSRSMLIA
Sbjct: 21  DDSSSPNSSSSVS------GVDSVLNPYYLHHTDNTELVLVTQPLTEENYSSWSRSMLIA 80

Query: 64  LTVKNKVGFIDGSLSRPTGEQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLD 123
           L++KNK+GFIDGS+SRP GE L +WI  N+VVI+WILNS+SKEIS+ +LF +SA +IW+D
Sbjct: 81  LSIKNKLGFIDGSISRPIGELLPAWIHNNHVVIAWILNSVSKEISSSILFSESARDIWID 140

Query: 124 LKERFQRQNRPLIFQFRRDLSNL 147
           LKERF++ N P IFQ +RDL+ L
Sbjct: 141 LKERFEKSNGPRIFQLKRDLAQL 157

[Close]

BLAST of Moc03g22510 vs. ExPASy TrEMBL
Match: A0A5J5BKC2 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_021321 PE=4 SV=1)

HSP 1 Score: 159.1 bits (401), Expect = 1.4e-35
Identity = 79/132 (59.85%), Postives = 101/132 (76.52%), Query Frame = 0


Query: 18  NQPQISLEHYINPYFLHHFDNTTLVLVSNLLTDENYTLWSRSMLIALTVKNKVGFIDGSL 77
           N  + ++E   NPY+LHH D+   +LVS  LT ENYT WSR+MLIAL+VKNK+GF+DGS+
Sbjct: 17  NSNRSAIEEPSNPYYLHHSDSLRQMLVSQQLTGENYTNWSRAMLIALSVKNKLGFVDGSI 76

Query: 78  SRPTG---EQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLDLKERFQRQNRP 137
             P G     L+SWI  NN+VISWILNS+SKEISA ++F  SA EIWLDL++RFQ++NRP
Sbjct: 77  LEPQGTGNNLLNSWIRNNNIVISWILNSVSKEISASIIFAASAREIWLDLRDRFQQRNRP 136

Query: 138 LIFQFRRDLSNL 147
            IFQ +R+L NL
Sbjct: 137 RIFQLKRELMNL 148

[Close]

BLAST of Moc03g22510 vs. TAIR 10
Match: AT1G21280.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Has 707 Blast hits to 705 proteins in 25 species: Archae - 0; Bacteria - 0; Metazoa - 4; Fungi - 0; Plants - 703; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 65.9 bits (159), Expect = 3.2e-11
Identity = 38/124 (30.65%), Postives = 66/124 (53.23%), Query Frame = 0


Query: 29  NPYFL----HHFDNTTLVLVSNLLTDENYTLWSRSMLIALTVKNKVGFIDGSLSRPT--G 88
           +PY+L    HH  + ++  +S    ++NY  W       L V  K GFIDG+L +P    
Sbjct: 16  SPYYLPPDIHHPSDFSIQKLSK--DEDNYVAWKIRFRSFLRVTKKFGFIDGTLPKPDPFS 75

Query: 89  EQLHSWIICNNVVISWILNSLSKEISARVLFFDSAYEIWLDLKERFQRQNRPLIFQFRRD 147
                W  CN +V+ W++NS++ ++   V++ ++A+++W DL+  F       I+Q RR 
Sbjct: 76  PLYQPWEQCNAMVMYWLMNSMTDKLLESVMYAETAHKMWEDLRRVFVPCVDLKIYQLRRR 135

The following BLAST results are available for this feature:

BLAST of Moc03g22510 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154919.1	2.4e-53	74.66	uncharacterized protein LOC111022065 [Momordica charantia]	[more]
XP_022152756.1	2.0e-52	75.51	uncharacterized protein LOC111020399 [Momordica charantia]	[more]
XP_022142327.1	7.5e-47	74.80	uncharacterized protein LOC111012468 [Momordica charantia]	[more]
XP_022154608.1	7.6e-39	58.04	uncharacterized protein LOC111021831 [Momordica charantia]	[more]
XP_038887186.1	1.7e-38	60.42	uncharacterized protein LOC120077373 [Benincasa hispida]	[more]

BLAST of Moc03g22510 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc03g22510 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DNP7	1.2e-53	74.66	uncharacterized protein LOC111022065 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1DIP8	9.9e-53	75.51	uncharacterized protein LOC111020399 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A6J1CMF8	3.7e-47	74.80	uncharacterized protein LOC111012468 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1DKR8	3.7e-39	58.04	uncharacterized protein LOC111021831 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A5J5BKC2	1.4e-35	59.85	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_021321 PE=4 SV=1	[more]

BLAST of Moc03g22510 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G21280.1	3.2e-11	30.65	CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); Ha...	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029472	Retrotransposon Copia-like, N-terminal	PFAM	PF14244	Retrotran_gag_3	coord: 34..80 e-value: 6.6E-20 score: 70.6
None	No IPR available	PANTHER	PTHR37610:SF53	SUBFAMILY NOT NAMED	coord: 24..145
None	No IPR available	PANTHER	PTHR37610	FAMILY NOT NAMED	coord: 24..145

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g22510.1	Moc03g22510.1	mRNA