Moc03g21070 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g21070
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionMajor facilitator superfamily protein
Locationchr3: 14391377 .. 14393889 (+)
RNA-Seq ExpressionMoc03g21070
SyntenyMoc03g21070
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTGTATCAACAATAAACCAGTCTAAGGGCGGTATTGGGAGTGGCGAAATGTCAAGTTTTATCCGGCAAGTGGTGGTGGGGCGGTGGTTTTCCGTTTTCGCTTCGTTTCTGATAATGTCCGGCGCCGGCGGAGTTTATTTGTTCGCTTATTACTCCAAAGACATAAAATCGACGCTCCGATGTGACCAAACGACGCTCAACAAGATCGGCTTTTACAAAGACCTCGGATCCAACGTCGGCGTCATCGCCGGACTACTGGCGGAGGTGGTGCCGACTTGGTTCCTTCTACTCATCGGCGCCGCCTTCAACTTCCTCGGCTACTTCAAGATATGGCAAGGCGTCACCGGAAAAATCGTGCGCCCGACCGTCGGCTACTTTTGCTTTTACATTATGATCGGTGCAAACTCTATAAACTTCGCTAATACAGGTAATGGTTTTACTCTAATAAATCAATCCACATCTCATAAATTCTTCACCTTTTGATTTTATGTTTAATAATCCATCAACTATTCAAACATGAAAGTGAAAATTTAAAACAAAAAATTCTTCTACTTTGAAAATGCTGCAAACCTGAACTGTGTTCAGATCAGAGCTCGAATTCCCATCCCAATCTGAGCATAGCTTGACTAGATCGAGTTCGAATTTTCCATCCCTATATATTGTACTAAAAAAAACTTTAAAATACTAAATACATCAGAGATCACTTTTTAGAAATTCAAGATTGTCCTATTAGATATAAAATTGAAATTTATAAGACAACTTACCTTCATCTTACTTAATAGTTATTTATAACCATGCAAAAAACATAACAGAACATTTTAATGAAAAGACTTTTAATAAGTTCATAACTACCGTTAAATTCCTTTTAAAGTTAAAAGGAGTCCATTACTGGTATGTGACATATTATTAAAACTTTAAGAATTTACTAGACATACTTTTAAATAGATAATACTTAGCTCTTAAGGACCAACTCATTTGTTTTAATTTCATTCAATCATGCAAGAGCAATTTAAGAATATAATAGACATATTTAAAAATGAATAGTACTTGGTTTGAATAATACTTTGGTTCGTTAACGGAACCAAATCCGATCCTAATAATAATTGAAAAATTGATAACTCATTTGTAACGTTTGGTTACGTTTGGTTGGTACCTGAACAGGAGTGTTGGTGACGTGCGTGAGGAACTTCCCAGAGAGAAGAGGGGTAATGTTGGGCCTACTGAAGGGCTTCGTGGGCCTAAGTGGGGCCATAATGACCCAACTCTACGTCGCCATTTACGGCCACGATACCAAATCTCTGATTCTGCTAATCGGCTGGTTCCCCTCTCTGCTCTCTCTGTTCTTCATCTACACAATCCGCGAACTGAGAGCCGAAAAACACCCAAACGAGTTCAGAGTTTTCGTCCAATTCCTCTGCGTCACCGTCATTTTAGCCGTCTTCCTCACCGTCTTGATCTTCATCCAGAAGCGCGTCCAGCTCGATCAATCAGCCCACATCGCAATCCTCGCCGCAATCTTCGCTCTGCTCTTCCTCCCCCTCCTGATCGCCATCAGAGAAGAATTGGTCCTCTGGAACCTCAAGAAAACCACCACCGGCAATTACTTCACCAGAATCAGAGTCGATCGGCCCGAGCCTCCCACCGCAACCACTTCATCGTCTTCCTCGGTCCAGACCCAGAGCAGCTCCTGCTTCGCCAACATTTTCAACAAGCCCGAGAGAGGCGAAGACTTCACCATCCTCCAAGCGATCTTGAGCGTCGACATGTTCATCCTCTTCATGTCGATGCTCGTCGGGGTCGGCTCGAGCCTGACCGCCATCGATAACCTCGGCCAGATCGGAGAATCCCTCGGCTACCCACACCAGACCATCAACTCCTTCATCTCCCTGGTCAGCATCTTCAACTTCACGGGCAGAATCTTCTCCGGGTTCGTCTCGGAAATCCTGCTCGAGAAGTACCAATTCCCGCGGCCGCTGATGCTGACGCTGATCCTCTTCGTGTCCTGCGTCGGCCATCTGGTAGTGGCGTTCCCGTTCGAGGACTCGCTCTACCTCGCCTCCGTCCTCATCGGGTTCTCCATGGGCTCCCAAGTGCCGCTCTACTTCGCGGTGATTTCGGAGATCTTCGGGCTGAAGCATTACTCGACGCTGTTCAATTTCGGGCAGCTGTCCTGCCCCGTCGGGTCGTACATTCTGAACGTGTTGGTGACCGGGAAGCTGTACGACGAGGTGGCGAAGACGGGGGTTGATCCGAAGGCGTTGCAGTGCCACGGGGTGCATTGTTTCCGGCAATCGTTTACGATTCTGGCGGCGCTGACGTTTGTTGTAGCGGTGCTGACGCTGGTTCTGGTGATGCGGACGAGCGAGTTCTACAGAGGGGATATATACAAGAAGTTCCGGGAGGATATGGACACGTTGAAGACGGAAGTTGAGTTCTATCGAGTTGACGAGAAGAGGACCAAGATTGGGAATTTGTTTGTTGACAAGCATACTATTAATTTCAAGAAGTAA

mRNA sequence

ATGATTGTATCAACAATAAACCAGTCTAAGGGCGGTATTGGGAGTGGCGAAATGTCAAGTTTTATCCGGCAAGTGGTGGTGGGGCGGTGGTTTTCCGTTTTCGCTTCGTTTCTGATAATGTCCGGCGCCGGCGGAGTTTATTTGTTCGCTTATTACTCCAAAGACATAAAATCGACGCTCCGATGTGACCAAACGACGCTCAACAAGATCGGCTTTTACAAAGACCTCGGATCCAACGTCGGCGTCATCGCCGGACTACTGGCGGAGGTGGTGCCGACTTGGTTCCTTCTACTCATCGGCGCCGCCTTCAACTTCCTCGGCTACTTCAAGATATGGCAAGGCGTCACCGGAAAAATCGTGCGCCCGACCGTCGGCTACTTTTGCTTTTACATTATGATCGGTGCAAACTCTATAAACTTCGCTAATACAGGAGTGTTGGTGACGTGCGTGAGGAACTTCCCAGAGAGAAGAGGGGTAATGTTGGGCCTACTGAAGGGCTTCGTGGGCCTAAGTGGGGCCATAATGACCCAACTCTACGTCGCCATTTACGGCCACGATACCAAATCTCTGATTCTGCTAATCGGCTGGTTCCCCTCTCTGCTCTCTCTGTTCTTCATCTACACAATCCGCGAACTGAGAGCCGAAAAACACCCAAACGAGTTCAGAGTTTTCGTCCAATTCCTCTGCGTCACCGTCATTTTAGCCGTCTTCCTCACCGTCTTGATCTTCATCCAGAAGCGCGTCCAGCTCGATCAATCAGCCCACATCGCAATCCTCGCCGCAATCTTCGCTCTGCTCTTCCTCCCCCTCCTGATCGCCATCAGAGAAGAATTGGTCCTCTGGAACCTCAAGAAAACCACCACCGGCAATTACTTCACCAGAATCAGAGTCGATCGGCCCGAGCCTCCCACCGCAACCACTTCATCGTCTTCCTCGGTCCAGACCCAGAGCAGCTCCTGCTTCGCCAACATTTTCAACAAGCCCGAGAGAGGCGAAGACTTCACCATCCTCCAAGCGATCTTGAGCGTCGACATGTTCATCCTCTTCATGTCGATGCTCGTCGGGGTCGGCTCGAGCCTGACCGCCATCGATAACCTCGGCCAGATCGGAGAATCCCTCGGCTACCCACACCAGACCATCAACTCCTTCATCTCCCTGGTCAGCATCTTCAACTTCACGGGCAGAATCTTCTCCGGGTTCGTCTCGGAAATCCTGCTCGAGAAGTACCAATTCCCGCGGCCGCTGATGCTGACGCTGATCCTCTTCGTGTCCTGCGTCGGCCATCTGGTAGTGGCGTTCCCGTTCGAGGACTCGCTCTACCTCGCCTCCGTCCTCATCGGGTTCTCCATGGGCTCCCAAGTGCCGCTCTACTTCGCGGTGATTTCGGAGATCTTCGGGCTGAAGCATTACTCGACGCTGTTCAATTTCGGGCAGCTGTCCTGCCCCGTCGGGTCGTACATTCTGAACGTGTTGGTGACCGGGAAGCTGTACGACGAGGTGGCGAAGACGGGGGTTGATCCGAAGGCGTTGCAGTGCCACGGGGTGCATTGTTTCCGGCAATCGTTTACGATTCTGGCGGCGCTGACGTTTGTTGTAGCGGTGCTGACGCTGGTTCTGGTGATGCGGACGAGCGAGTTCTACAGAGGGGATATATACAAGAAGTTCCGGGAGGATATGGACACGTTGAAGACGGAAGTTGAGTTCTATCGAGTTGACGAGAAGAGGACCAAGATTGGGAATTTGTTTGTTGACAAGCATACTATTAATTTCAAGAAGTAA

Coding sequence (CDS)

ATGATTGTATCAACAATAAACCAGTCTAAGGGCGGTATTGGGAGTGGCGAAATGTCAAGTTTTATCCGGCAAGTGGTGGTGGGGCGGTGGTTTTCCGTTTTCGCTTCGTTTCTGATAATGTCCGGCGCCGGCGGAGTTTATTTGTTCGCTTATTACTCCAAAGACATAAAATCGACGCTCCGATGTGACCAAACGACGCTCAACAAGATCGGCTTTTACAAAGACCTCGGATCCAACGTCGGCGTCATCGCCGGACTACTGGCGGAGGTGGTGCCGACTTGGTTCCTTCTACTCATCGGCGCCGCCTTCAACTTCCTCGGCTACTTCAAGATATGGCAAGGCGTCACCGGAAAAATCGTGCGCCCGACCGTCGGCTACTTTTGCTTTTACATTATGATCGGTGCAAACTCTATAAACTTCGCTAATACAGGAGTGTTGGTGACGTGCGTGAGGAACTTCCCAGAGAGAAGAGGGGTAATGTTGGGCCTACTGAAGGGCTTCGTGGGCCTAAGTGGGGCCATAATGACCCAACTCTACGTCGCCATTTACGGCCACGATACCAAATCTCTGATTCTGCTAATCGGCTGGTTCCCCTCTCTGCTCTCTCTGTTCTTCATCTACACAATCCGCGAACTGAGAGCCGAAAAACACCCAAACGAGTTCAGAGTTTTCGTCCAATTCCTCTGCGTCACCGTCATTTTAGCCGTCTTCCTCACCGTCTTGATCTTCATCCAGAAGCGCGTCCAGCTCGATCAATCAGCCCACATCGCAATCCTCGCCGCAATCTTCGCTCTGCTCTTCCTCCCCCTCCTGATCGCCATCAGAGAAGAATTGGTCCTCTGGAACCTCAAGAAAACCACCACCGGCAATTACTTCACCAGAATCAGAGTCGATCGGCCCGAGCCTCCCACCGCAACCACTTCATCGTCTTCCTCGGTCCAGACCCAGAGCAGCTCCTGCTTCGCCAACATTTTCAACAAGCCCGAGAGAGGCGAAGACTTCACCATCCTCCAAGCGATCTTGAGCGTCGACATGTTCATCCTCTTCATGTCGATGCTCGTCGGGGTCGGCTCGAGCCTGACCGCCATCGATAACCTCGGCCAGATCGGAGAATCCCTCGGCTACCCACACCAGACCATCAACTCCTTCATCTCCCTGGTCAGCATCTTCAACTTCACGGGCAGAATCTTCTCCGGGTTCGTCTCGGAAATCCTGCTCGAGAAGTACCAATTCCCGCGGCCGCTGATGCTGACGCTGATCCTCTTCGTGTCCTGCGTCGGCCATCTGGTAGTGGCGTTCCCGTTCGAGGACTCGCTCTACCTCGCCTCCGTCCTCATCGGGTTCTCCATGGGCTCCCAAGTGCCGCTCTACTTCGCGGTGATTTCGGAGATCTTCGGGCTGAAGCATTACTCGACGCTGTTCAATTTCGGGCAGCTGTCCTGCCCCGTCGGGTCGTACATTCTGAACGTGTTGGTGACCGGGAAGCTGTACGACGAGGTGGCGAAGACGGGGGTTGATCCGAAGGCGTTGCAGTGCCACGGGGTGCATTGTTTCCGGCAATCGTTTACGATTCTGGCGGCGCTGACGTTTGTTGTAGCGGTGCTGACGCTGGTTCTGGTGATGCGGACGAGCGAGTTCTACAGAGGGGATATATACAAGAAGTTCCGGGAGGATATGGACACGTTGAAGACGGAAGTTGAGTTCTATCGAGTTGACGAGAAGAGGACCAAGATTGGGAATTTGTTTGTTGACAAGCATACTATTAATTTCAAGAAGTAA

Protein sequence

MIVSTINQSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDRPEPPTATTSSSSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK
Homology
BLAST of Moc03g21070 vs. NCBI nr
Match: XP_038895750.1 (uncharacterized protein LOC120083915 [Benincasa hispida])

HSP 1 Score: 941.4 bits (2432), Expect = 3.7e-270
Identity = 469/600 (78.17%), Postives = 537/600 (89.50%), Query Frame = 0

Query: 1   MIVSTINQSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTL 60
           MIV+  + S GGIGSGE++ F+RQVVVGRWFS+FASFL+MSGAGGVY+FAYYSKDIK+TL
Sbjct: 1   MIVAAGHSSNGGIGSGELADFVRQVVVGRWFSLFASFLVMSGAGGVYVFAYYSKDIKTTL 60

Query: 61  RCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIV 120
           +CDQTTLNKIGFYKDLGSNVG++AGLLAEV PTW +LLIGAA NFLGYFKIWQ VTGKIV
Sbjct: 61  KCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWLVLLIGAALNFLGYFKIWQAVTGKIV 120

Query: 121 RPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYV 180
            PTVG+FCF+IM+GANS NFANTGVLVTCV+NFPERRGVMLGLLKGFVGLSGAIMTQLY+
Sbjct: 121 HPTVGFFCFFIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTV 240
           AIYGHDT+SL+LLI WFPSL+SL F+YTIRE++  KHPNEFRVFVQFLC+T++L+VFLTV
Sbjct: 181 AIYGHDTRSLVLLIAWFPSLISLIFVYTIREIKFVKHPNEFRVFVQFLCLTILLSVFLTV 240

Query: 241 LIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNL-KKTTTGNYFTRIRVDR 300
           LIFIQKRVQ DQSAHIAI+AAIFALLF+PLLIAIREELVLWN  K+T   N FTRIR++ 
Sbjct: 241 LIFIQKRVQFDQSAHIAIVAAIFALLFVPLLIAIREELVLWNFNKRTLINNPFTRIRIET 300

Query: 301 PEPPTATTS-------SSSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSM 360
           P+  T TT+       SS+  Q Q SSCFANIFNKPERGED+T+LQAI S+DM I+  +M
Sbjct: 301 PQTTTTTTTTTNPPIPSSNQTQIQPSSCFANIFNKPERGEDYTVLQAIFSIDMLIICSTM 360

Query: 361 LVGVGSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFP 420
           L+GVG+SLTAIDNLGQIGES GYP  TIN FISLVSIFNFTGRIFSGFVSEILLEK++FP
Sbjct: 361 LIGVGASLTAIDNLGQIGESQGYPSGTINLFISLVSIFNFTGRIFSGFVSEILLEKFKFP 420

Query: 421 RPLMLTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYST 480
           RPLMLT IL +SCVGHL+VAFPFEDSLY+AS++IGFSMGSQVPL+FA+ISEIFGLKHYST
Sbjct: 421 RPLMLTFILLISCVGHLLVAFPFEDSLYIASIIIGFSMGSQVPLHFAMISEIFGLKHYST 480

Query: 481 LFNFGQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVV 540
           LFNFGQLSCP+GSYILNV+VTGKLYDEVA+ G  P    C G HC+ QSF IL+ LTF+V
Sbjct: 481 LFNFGQLSCPIGSYILNVMVTGKLYDEVAQLGSSPNKFHCQGNHCYEQSFLILSGLTFIV 540

Query: 541 AVLTLVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           A+++L+LV RT EFY GDIYKKFREDM+TLKTE+EFYR+DEKRT+IGNL VDKH+INFK+
Sbjct: 541 AMISLILVKRTREFYGGDIYKKFREDMETLKTEMEFYRLDEKRTRIGNLLVDKHSINFKR 600

BLAST of Moc03g21070 vs. NCBI nr
Match: XP_008464809.1 (PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo])

HSP 1 Score: 920.6 bits (2378), Expect = 6.8e-264
Identity = 462/596 (77.52%), Postives = 534/596 (89.60%), Query Frame = 0

Query: 1   MIVSTINQSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTL 60
           MIV+   +S GGI SGE++ FI QVVVGRWFS+FASFL+M+GAGGVYLFAYYS+DIK+TL
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  RCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIV 120
           +CDQTTLN+IGFYKDLGSNVGVIAGLLAEV PTW +LLIGAAFNF+GYFKIWQ VTGKIV
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 RPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYV 180
           RPTV +FCFYIMIGANS NFANTGVLVTCV+NFPERRGVMLGLLKGFVGLSGAIMTQLY+
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTV 240
           AIYGHDTKSLILLI WFPS++SL F+YTIRE++  KHPNEFRVFVQFLCVTV+LA+ LT 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNL-KKTTTGNYFTRIRVD- 300
           LIF+QKRV  DQSAHIAI+AAI ALLF+PLLIAIREE+V+WNL K+T   N F RI ++ 
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 -RPEPPTATTSS-SSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGV 360
            +P+PP  + S+    +QTQ +SCF+NIFNKPERGED+T+LQAI S+DM I+  +ML+GV
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLM 420
           G+SLTAIDNLGQIGE+ GYP +TIN F+SL+SI NFTGRIFSGFVSEILLEK+QFPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNF 480
           LTLIL +S +GHL+VAFPF+DSLYLAS++IGFSMGSQVPL+FA+ISEIFGLKHYSTLFNF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLT 540
           GQLSCP+GSYILNV+VTGKLYDEVA+ G +P    C G HC+ QSF ILA LTF+VA+++
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMIS 540

Query: 541 LVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           L+LV RT EFYRGDIYKKF+EDM+TLKTEVEFYRVDEKRT+IGNL VDKH+INFKK
Sbjct: 541 LILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 596

BLAST of Moc03g21070 vs. NCBI nr
Match: KAA0051898.1 (protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa])

HSP 1 Score: 916.8 bits (2368), Expect = 9.8e-263
Identity = 459/589 (77.93%), Postives = 530/589 (89.98%), Query Frame = 0

Query: 8   QSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTL 67
           +S GGI SGE++ FI QVVVGRWFS+FASFL+M+GAGGVYLFAYYS+DIK+TL+CDQTTL
Sbjct: 33  RSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTL 92

Query: 68  NKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYF 127
           N+IGFYKDLGSNVGVIAGLLAEV PTW +LLIGAAFNF+GYFKIWQ VTGKIVRPTV +F
Sbjct: 93  NRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFF 152

Query: 128 CFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDT 187
           CFYIMIGANS NFANTGVLVTCV+NFPERRGVMLGLLKGFVGLSGAIMTQLY+AIYGHDT
Sbjct: 153 CFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDT 212

Query: 188 KSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKR 247
           KSLILLI WFPS++SL F+YTIRE++  KHPNEFRVFVQFLCVTV+LA+ LT LIF+QKR
Sbjct: 213 KSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKR 272

Query: 248 VQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNL-KKTTTGNYFTRIRVD--RPEPPT 307
           V  DQSAHIAI+AAI ALLF+PLLIAIREE+V+WNL K+T   N F RI ++  +P+PP 
Sbjct: 273 VHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPI 332

Query: 308 ATTSS-SSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAI 367
            + S+    +QTQ +SCF+NIFNKPERGED+T+LQAI S+DM I+  +ML+GVG+SLTAI
Sbjct: 333 HSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAI 392

Query: 368 DNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFV 427
           DNLGQIGE+ GYP +TIN F+SL+SI NFTGRIFSGFVSEILLEK+QFPRPLMLTLIL +
Sbjct: 393 DNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLI 452

Query: 428 SCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPV 487
           S +GHL+VAFPF+DSLYLAS++IGFSMGSQVPL+FA+ISEIFGLKHYSTLFNFGQLSCP+
Sbjct: 453 SSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPI 512

Query: 488 GSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLVMRT 547
           GSYILNV+VTGKLYDEVA+ G +P    C G HC+ QSF ILA LTF+VA+++L+LV RT
Sbjct: 513 GSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRT 572

Query: 548 SEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
            EFYRGDIYKKF+EDM+TLKTEVEFYRVDEKRT+IGNL VDKH+INFKK
Sbjct: 573 REFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 621

BLAST of Moc03g21070 vs. NCBI nr
Match: XP_004147303.3 (uncharacterized protein LOC101202941 [Cucumis sativus] >KGN64782.2 hypothetical protein Csa_013229 [Cucumis sativus])

HSP 1 Score: 915.2 bits (2364), Expect = 2.9e-262
Identity = 455/600 (75.83%), Postives = 531/600 (88.50%), Query Frame = 0

Query: 1   MIVSTINQSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTL 60
           MIV+   +S GGIGSGE+  F+RQ+V GRWFS+FASFL+M+GAGGVYLFAYYS+DIK+TL
Sbjct: 1   MIVAAGQRSNGGIGSGELCDFVRQMVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  RCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIV 120
           +CDQTTLNKIGFYKDLGSNVGV AGLLAEVVPTW LLLIGAAFNF+GYFKIWQ VTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGVFAGLLAEVVPTWLLLLIGAAFNFMGYFKIWQAVTGKIV 120

Query: 121 RPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYV 180
           RPTV +FCFYIMIGANS NFANTGVLVTCV+NFPERRGVMLGLLKGFVGLSGAIMTQ Y+
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQFYI 180

Query: 181 AIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTV 240
           AIYGHDTKSL+LL+ WFPSL+SL F+YTIRE+++ KHPNEFRVF+QFLCVTV+L + LTV
Sbjct: 181 AIYGHDTKSLVLLLAWFPSLISLLFVYTIREIKSVKHPNEFRVFIQFLCVTVLLTILLTV 240

Query: 241 LIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNL-KKTTTGNYFTRIRVDR 300
           +IFIQKR+  DQSAHIAI+AAI ALLF+PLLIAIREE+VLWNL K+T   N FTRI+++ 
Sbjct: 241 IIFIQKRIHFDQSAHIAIVAAILALLFVPLLIAIREEVVLWNLNKRTRISNPFTRIKIET 300

Query: 301 PEPPTATTSSSSS-------VQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSM 360
            +  +   S S+S        QTQ +SCF+ IFNKPERGED+T+LQAI S+DM I+  +M
Sbjct: 301 SQTNSPVDSPSTSQHPHPHPPQTQPTSCFSKIFNKPERGEDYTVLQAIFSIDMLIICFTM 360

Query: 361 LVGVGSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFP 420
           ++GVG+SLTAIDNLGQIGE+  Y  +TIN  +SL+SIFNF GRIFSGFVSEILLEK+QFP
Sbjct: 361 MIGVGASLTAIDNLGQIGEAQAYSSETINLLVSLMSIFNFAGRIFSGFVSEILLEKFQFP 420

Query: 421 RPLMLTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYST 480
           RPLMLTLIL +SC+GHL+VAFPF+DSLY+AS++IGFSMGSQVPL+FA+ISEIFGLKHYST
Sbjct: 421 RPLMLTLILLISCLGHLLVAFPFDDSLYVASIIIGFSMGSQVPLHFAMISEIFGLKHYST 480

Query: 481 LFNFGQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVV 540
           LFNFGQLSCP+GSYILNVLVTGKLYDEVA+ G +P  L C G HC+ +SF ILA LTF+V
Sbjct: 481 LFNFGQLSCPIGSYILNVLVTGKLYDEVARIGSNPNMLHCVGTHCYERSFLILAGLTFMV 540

Query: 541 AVLTLVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           A+++L+LV RT EFYRGDIYKKFREDM+TLKTEVEFYRVDEK+T+IGNL VDKH+I+FKK
Sbjct: 541 AMVSLILVKRTREFYRGDIYKKFREDMETLKTEVEFYRVDEKKTRIGNLLVDKHSIHFKK 600

BLAST of Moc03g21070 vs. NCBI nr
Match: XP_022998867.1 (uncharacterized protein LOC111493395 [Cucurbita maxima])

HSP 1 Score: 887.1 bits (2291), Expect = 8.3e-254
Identity = 455/598 (76.09%), Postives = 520/598 (86.96%), Query Frame = 0

Query: 1   MIVSTINQS-KGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKST 60
           MIVS    S  GGI   E+S FI+QVV GRWFS+FASFLIMSGAGGVYLFA YSKDIKST
Sbjct: 1   MIVSPPPSSINGGICRAELSCFIQQVVTGRWFSLFASFLIMSGAGGVYLFASYSKDIKST 60

Query: 61  LRCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKI 120
           L+CDQTTLNKIGFYKDLGSNVGVIAGLLAEV PTWF++LIGAA NF+GYFKIWQ VTGKI
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWFVILIGAALNFVGYFKIWQAVTGKI 120

Query: 121 VRPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLY 180
           +RPTV +FCFYIM+GANS NFANT VLV+CV+NFPERRGVMLGLLKGFVGLSGAIMTQ+Y
Sbjct: 121 IRPTVEFFCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIY 180

Query: 181 VAIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLT 240
            A Y HDTKSLILL+ WFPSL+S+ F+ TIRE+RA KHPNEFRVFVQFLCVT+ILA+FLT
Sbjct: 181 GAFYDHDTKSLILLLAWFPSLISIIFVKTIREMRAVKHPNEFRVFVQFLCVTIILALFLT 240

Query: 241 VLIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDR 300
            LIF+QK+VQ +Q+AHI I+AAI ALL +PLLIAIREE+++WNL K T GN FTRIR+D 
Sbjct: 241 ALIFVQKKVQFNQTAHITIVAAILALLLVPLLIAIREEIIMWNLNKRTVGNRFTRIRIDN 300

Query: 301 PE--PPTATTSSSSSV---QTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLV 360
           P+  P  + + SSSSV     QS+SCFANIFNKP+RGEDFTI QAI SVDM IL  +ML+
Sbjct: 301 PQSAPSCSASCSSSSVLELPKQSNSCFANIFNKPKRGEDFTIPQAIFSVDMLILLTTMLI 360

Query: 361 GVGSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRP 420
           GVG++LTAIDNLGQIGESL YP +TINSFISLVSIFNFTGRIFSGFVSEILLEK++FPRP
Sbjct: 361 GVGANLTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKFPRP 420

Query: 421 LMLTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLF 480
           +MLTLI  VSCVG+L+VAFPF +SL +AS++IGFS+GSQVPL+FA+ISE FGLKHYSTLF
Sbjct: 421 MMLTLIHLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYSTLF 480

Query: 481 NFGQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAV 540
           NFGQLSCP+GSYILNV+V GKLYDE+A TG +     C G  CF QSFTILA LTFVVA+
Sbjct: 481 NFGQLSCPIGSYILNVMVIGKLYDEMALTGNNASEFHCEGSKCFEQSFTILAGLTFVVAM 540

Query: 541 LTLVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           ++LVLV RT+EFYRGDIY+KFREDMD+LKTE+EFY ++ KR KIGNL  DKH INFKK
Sbjct: 541 VSLVLVRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFKK 598

BLAST of Moc03g21070 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 124.4 bits (311), Expect = 4.3e-27
Identity = 126/537 (23.46%), Postives = 230/537 (42.83%), Query Frame = 0

Query: 29  RWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGFYKDLGSNVGVIAGLLA 88
           +W  + A+  I +  G  + F+ YS  +KS L   Q  LN +    DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 89  EVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYFCFYIMIGANSINFANTGVLVT 148
              P   +L   AA  F+GY   W  +T  I  P    F   ++ G  SI + NT   + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLPYSLVFLCCLLAGL-SICWFNTACFIL 162

Query: 149 CVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDTKSLILLIGWFPSLLSLFFIY- 208
           C+R+FP  R + L L   F G+S A+ +  + AI    +   +LL    P ++S   +Y 
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAINPSSSNLYLLLNSLVPLVVSFAALYP 222

Query: 209 --------TIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKRVQLDQSAHIAIL 268
                   T  +  + +H +     +  L V     + L+             SA +  +
Sbjct: 223 VLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLS--------SSSTSSARLNFI 282

Query: 269 AAIFALLF---LPLLIAIREE-LVLWNLKKTTTGNYFTRIRVDRPEPPTATTSSSSSVQT 328
            A+  L+F    PLL+  R+  L + N +     + +  + +D  +    + SS +  + 
Sbjct: 283 GAVVLLVFPLCAPLLVYARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTGYEH 342

Query: 329 QSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAIDNLGQIGESLGY 388
             +   A   N    G++ +    I  ++ ++ +++   G    L   +NLGQI +SLG 
Sbjct: 343 MGT---AKEGNTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG- 402

Query: 389 PHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFVSCVGH--LVVAF 448
             Q   + +++ S F+F GR+ S    + + ++++  R     + L  + +    L V+ 
Sbjct: 403 --QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSS 462

Query: 449 PFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPVGSYILNVLVT 508
             + +L  A+ LIG S G       ++ S++FG        N    + P+GS +L   + 
Sbjct: 463 SQQTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGS-LLYGYIA 522

Query: 509 GKLYDEVAKTGVDP---KALQCHGVHCFRQSFTILAALTFVVAVLTLVLVMRTSEFY 548
             +Y+  A   + P    ++ C G  C+ ++F     L+ +  V +L L +RT   Y
Sbjct: 523 ASIYEANASPDITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

BLAST of Moc03g21070 vs. ExPASy TrEMBL
Match: A0A1S3CMG9 (uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=4 SV=1)

HSP 1 Score: 920.6 bits (2378), Expect = 3.3e-264
Identity = 462/596 (77.52%), Postives = 534/596 (89.60%), Query Frame = 0

Query: 1   MIVSTINQSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTL 60
           MIV+   +S GGI SGE++ FI QVVVGRWFS+FASFL+M+GAGGVYLFAYYS+DIK+TL
Sbjct: 1   MIVAAGQRSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTL 60

Query: 61  RCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIV 120
           +CDQTTLN+IGFYKDLGSNVGVIAGLLAEV PTW +LLIGAAFNF+GYFKIWQ VTGKIV
Sbjct: 61  QCDQTTLNRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIV 120

Query: 121 RPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYV 180
           RPTV +FCFYIMIGANS NFANTGVLVTCV+NFPERRGVMLGLLKGFVGLSGAIMTQLY+
Sbjct: 121 RPTVAFFCFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYI 180

Query: 181 AIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTV 240
           AIYGHDTKSLILLI WFPS++SL F+YTIRE++  KHPNEFRVFVQFLCVTV+LA+ LT 
Sbjct: 181 AIYGHDTKSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTA 240

Query: 241 LIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNL-KKTTTGNYFTRIRVD- 300
           LIF+QKRV  DQSAHIAI+AAI ALLF+PLLIAIREE+V+WNL K+T   N F RI ++ 
Sbjct: 241 LIFVQKRVHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIES 300

Query: 301 -RPEPPTATTSS-SSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGV 360
            +P+PP  + S+    +QTQ +SCF+NIFNKPERGED+T+LQAI S+DM I+  +ML+GV
Sbjct: 301 SQPKPPIHSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGV 360

Query: 361 GSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLM 420
           G+SLTAIDNLGQIGE+ GYP +TIN F+SL+SI NFTGRIFSGFVSEILLEK+QFPRPLM
Sbjct: 361 GASLTAIDNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLM 420

Query: 421 LTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNF 480
           LTLIL +S +GHL+VAFPF+DSLYLAS++IGFSMGSQVPL+FA+ISEIFGLKHYSTLFNF
Sbjct: 421 LTLILLISSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNF 480

Query: 481 GQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLT 540
           GQLSCP+GSYILNV+VTGKLYDEVA+ G +P    C G HC+ QSF ILA LTF+VA+++
Sbjct: 481 GQLSCPIGSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMIS 540

Query: 541 LVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           L+LV RT EFYRGDIYKKF+EDM+TLKTEVEFYRVDEKRT+IGNL VDKH+INFKK
Sbjct: 541 LILVQRTREFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 596

BLAST of Moc03g21070 vs. ExPASy TrEMBL
Match: A0A5D3BP48 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2042G00080 PE=4 SV=1)

HSP 1 Score: 916.8 bits (2368), Expect = 4.7e-263
Identity = 459/589 (77.93%), Postives = 530/589 (89.98%), Query Frame = 0

Query: 8   QSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTL 67
           +S GGI SGE++ FI QVVVGRWFS+FASFL+M+GAGGVYLFAYYS+DIK+TL+CDQTTL
Sbjct: 33  RSNGGIRSGELADFIGQVVVGRWFSLFASFLVMTGAGGVYLFAYYSRDIKTTLQCDQTTL 92

Query: 68  NKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYF 127
           N+IGFYKDLGSNVGVIAGLLAEV PTW +LLIGAAFNF+GYFKIWQ VTGKIVRPTV +F
Sbjct: 93  NRIGFYKDLGSNVGVIAGLLAEVAPTWLVLLIGAAFNFIGYFKIWQAVTGKIVRPTVAFF 152

Query: 128 CFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDT 187
           CFYIMIGANS NFANTGVLVTCV+NFPERRGVMLGLLKGFVGLSGAIMTQLY+AIYGHDT
Sbjct: 153 CFYIMIGANSQNFANTGVLVTCVKNFPERRGVMLGLLKGFVGLSGAIMTQLYIAIYGHDT 212

Query: 188 KSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKR 247
           KSLILLI WFPS++SL F+YTIRE++  KHPNEFRVFVQFLCVTV+LA+ LT LIF+QKR
Sbjct: 213 KSLILLIAWFPSIVSLIFVYTIREIKFVKHPNEFRVFVQFLCVTVLLALLLTALIFVQKR 272

Query: 248 VQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNL-KKTTTGNYFTRIRVD--RPEPPT 307
           V  DQSAHIAI+AAI ALLF+PLLIAIREE+V+WNL K+T   N F RI ++  +P+PP 
Sbjct: 273 VHFDQSAHIAIVAAILALLFVPLLIAIREEVVIWNLNKRTPISNPFIRIGIESSQPKPPI 332

Query: 308 ATTSS-SSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAI 367
            + S+    +QTQ +SCF+NIFNKPERGED+T+LQAI S+DM I+  +ML+GVG+SLTAI
Sbjct: 333 HSPSTPQHPLQTQPTSCFSNIFNKPERGEDYTVLQAIFSIDMLIICSTMLIGVGASLTAI 392

Query: 368 DNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFV 427
           DNLGQIGE+ GYP +TIN F+SL+SI NFTGRIFSGFVSEILLEK+QFPRPLMLTLIL +
Sbjct: 393 DNLGQIGEAQGYPSETINLFVSLISISNFTGRIFSGFVSEILLEKFQFPRPLMLTLILLI 452

Query: 428 SCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPV 487
           S +GHL+VAFPF+DSLYLAS++IGFSMGSQVPL+FA+ISEIFGLKHYSTLFNFGQLSCP+
Sbjct: 453 SSLGHLLVAFPFDDSLYLASIIIGFSMGSQVPLHFAMISEIFGLKHYSTLFNFGQLSCPI 512

Query: 488 GSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLVMRT 547
           GSYILNV+VTGKLYDEVA+ G +P    C G HC+ QSF ILA LTF+VA+++L+LV RT
Sbjct: 513 GSYILNVMVTGKLYDEVARIGSNPNKSHCVGTHCYEQSFLILAGLTFIVAMISLILVQRT 572

Query: 548 SEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
            EFYRGDIYKKF+EDM+TLKTEVEFYRVDEKRT+IGNL VDKH+INFKK
Sbjct: 573 REFYRGDIYKKFKEDMETLKTEVEFYRVDEKRTRIGNLLVDKHSINFKK 621

BLAST of Moc03g21070 vs. ExPASy TrEMBL
Match: A0A6J1K965 (uncharacterized protein LOC111493395 OS=Cucurbita maxima OX=3661 GN=LOC111493395 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 4.0e-254
Identity = 455/598 (76.09%), Postives = 520/598 (86.96%), Query Frame = 0

Query: 1   MIVSTINQS-KGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKST 60
           MIVS    S  GGI   E+S FI+QVV GRWFS+FASFLIMSGAGGVYLFA YSKDIKST
Sbjct: 1   MIVSPPPSSINGGICRAELSCFIQQVVTGRWFSLFASFLIMSGAGGVYLFASYSKDIKST 60

Query: 61  LRCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKI 120
           L+CDQTTLNKIGFYKDLGSNVGVIAGLLAEV PTWF++LIGAA NF+GYFKIWQ VTGKI
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPTWFVILIGAALNFVGYFKIWQAVTGKI 120

Query: 121 VRPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLY 180
           +RPTV +FCFYIM+GANS NFANT VLV+CV+NFPERRGVMLGLLKGFVGLSGAIMTQ+Y
Sbjct: 121 IRPTVEFFCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIY 180

Query: 181 VAIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLT 240
            A Y HDTKSLILL+ WFPSL+S+ F+ TIRE+RA KHPNEFRVFVQFLCVT+ILA+FLT
Sbjct: 181 GAFYDHDTKSLILLLAWFPSLISIIFVKTIREMRAVKHPNEFRVFVQFLCVTIILALFLT 240

Query: 241 VLIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDR 300
            LIF+QK+VQ +Q+AHI I+AAI ALL +PLLIAIREE+++WNL K T GN FTRIR+D 
Sbjct: 241 ALIFVQKKVQFNQTAHITIVAAILALLLVPLLIAIREEIIMWNLNKRTVGNRFTRIRIDN 300

Query: 301 PE--PPTATTSSSSSV---QTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLV 360
           P+  P  + + SSSSV     QS+SCFANIFNKP+RGEDFTI QAI SVDM IL  +ML+
Sbjct: 301 PQSAPSCSASCSSSSVLELPKQSNSCFANIFNKPKRGEDFTIPQAIFSVDMLILLTTMLI 360

Query: 361 GVGSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRP 420
           GVG++LTAIDNLGQIGESL YP +TINSFISLVSIFNFTGRIFSGFVSEILLEK++FPRP
Sbjct: 361 GVGANLTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKFPRP 420

Query: 421 LMLTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLF 480
           +MLTLI  VSCVG+L+VAFPF +SL +AS++IGFS+GSQVPL+FA+ISE FGLKHYSTLF
Sbjct: 421 MMLTLIHLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYSTLF 480

Query: 481 NFGQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAV 540
           NFGQLSCP+GSYILNV+V GKLYDE+A TG +     C G  CF QSFTILA LTFVVA+
Sbjct: 481 NFGQLSCPIGSYILNVMVIGKLYDEMALTGNNASEFHCEGSKCFEQSFTILAGLTFVVAM 540

Query: 541 LTLVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           ++LVLV RT+EFYRGDIY+KFREDMD+LKTE+EFY ++ KR KIGNL  DKH INFKK
Sbjct: 541 VSLVLVRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFKK 598

BLAST of Moc03g21070 vs. ExPASy TrEMBL
Match: A0A6J1GAT5 (uncharacterized protein LOC111452462 OS=Cucurbita moschata OX=3662 GN=LOC111452462 PE=4 SV=1)

HSP 1 Score: 887.1 bits (2291), Expect = 4.0e-254
Identity = 456/598 (76.25%), Postives = 521/598 (87.12%), Query Frame = 0

Query: 1   MIVSTINQS-KGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKST 60
           MIVS    S  GGI   E+S FI+QVV+GRWFS+FASFLIMSGAGGVYLFA YSKDIKST
Sbjct: 1   MIVSPPPSSINGGICRAELSCFIQQVVMGRWFSLFASFLIMSGAGGVYLFASYSKDIKST 60

Query: 61  LRCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKI 120
           L+CDQTTLNKIGFYKDLGSNVGVIAGLLAEV P WF++LIGAA NF+GYFKIWQ VTGKI
Sbjct: 61  LKCDQTTLNKIGFYKDLGSNVGVIAGLLAEVAPPWFVILIGAALNFVGYFKIWQAVTGKI 120

Query: 121 VRPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLY 180
           +RPTV +FCFYIM+GANS NFANT VLV+CV+NFPERRGVMLGLLKGFVGLSGAIMTQ+Y
Sbjct: 121 IRPTVEFFCFYIMVGANSQNFANTVVLVSCVKNFPERRGVMLGLLKGFVGLSGAIMTQIY 180

Query: 181 VAIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLT 240
            AIY HDTKSLILL+ WFPSL+S+ F+ TIRE+RA KHPNEFRVFVQFLCVTVILA+FLT
Sbjct: 181 GAIYDHDTKSLILLLAWFPSLISIVFVKTIREMRAVKHPNEFRVFVQFLCVTVILALFLT 240

Query: 241 VLIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDR 300
            LIF+QK+V  +Q+AHI I+AAIFALL +PLLIAIREE+++WNL K T GN FTRIR+D 
Sbjct: 241 ALIFVQKKVPFNQTAHITIVAAIFALLLVPLLIAIREEIIMWNLNKRTVGNRFTRIRIDN 300

Query: 301 PE--PPTATTSSSSSV---QTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLV 360
           P+  P  + + SSSSV     QS+SCFA IFNKP+RGEDFTI QAI SVDM IL  +ML+
Sbjct: 301 PQSAPSCSASCSSSSVLEQPKQSNSCFAAIFNKPKRGEDFTIPQAIFSVDMLILLTTMLI 360

Query: 361 GVGSSLTAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRP 420
           GVG++LTAIDNLGQIGESL YP +TINSFISLVSIFNFTGRIFSGFVSEILLEK++FPRP
Sbjct: 361 GVGANLTAIDNLGQIGESLNYPPETINSFISLVSIFNFTGRIFSGFVSEILLEKFKFPRP 420

Query: 421 LMLTLILFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLF 480
           +MLTLIL VSCVG+L+VAFPF +SL +AS++IGFS+GSQVPL+FA+ISE FGLKHYSTLF
Sbjct: 421 MMLTLILLVSCVGYLIVAFPFNNSLCIASIIIGFSLGSQVPLHFAMISEFFGLKHYSTLF 480

Query: 481 NFGQLSCPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAV 540
           NFGQLSCP+GSYILNV+V GKLYDE+A TG +     C G  CF QSFTILA LTFVVA+
Sbjct: 481 NFGQLSCPIGSYILNVMVIGKLYDEMAMTGSNASKFHCEGSKCFEQSFTILAGLTFVVAM 540

Query: 541 LTLVLVMRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFKK 593
           ++LVLV RT+EFYRGDIY+KFREDMD+LKTE+EFY ++ KR KIGNL  DKH INFKK
Sbjct: 541 VSLVLVRRTAEFYRGDIYQKFREDMDSLKTEMEFYPLESKRMKIGNLTFDKHAINFKK 598

BLAST of Moc03g21070 vs. ExPASy TrEMBL
Match: A0A6J1HLR3 (uncharacterized protein LOC111464761 OS=Cucurbita moschata OX=3662 GN=LOC111464761 PE=4 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.5e-242
Identity = 431/591 (72.93%), Postives = 498/591 (84.26%), Query Frame = 0

Query: 1   MIVSTINQSKGGIGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTL 60
           MIVS    S  GIGSGE+ SF+RQVV GRWFS+FASFL+M+GAGGVYLFAYYS+DIK+TL
Sbjct: 1   MIVSATPSSNAGIGSGELGSFVRQVVAGRWFSLFASFLVMAGAGGVYLFAYYSRDIKTTL 60

Query: 61  RCDQTTLNKIGFYKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIV 120
           +CDQTTLNKIGFYKDLGSNVG++AGLLAEV PTW LLLIGAA NF+GYFKIWQGVTGKIV
Sbjct: 61  QCDQTTLNKIGFYKDLGSNVGIVAGLLAEVAPTWVLLLIGAALNFVGYFKIWQGVTGKIV 120

Query: 121 RPTVGYFCFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYV 180
            PTV YFCFYIM+GANS NFANTGVLVTCV+NFPERRGVMLGL KGFVGLSGAIMTQ+Y 
Sbjct: 121 SPTVAYFCFYIMVGANSQNFANTGVLVTCVKNFPERRGVMLGLAKGFVGLSGAIMTQIYT 180

Query: 181 AIYGHDTKSLILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTV 240
           AIYG DT+SLILL+GW PSL+SL FI TIRE++A KHPNEFRVFV FLCV+VILA+FL  
Sbjct: 181 AIYGDDTRSLILLLGWLPSLISLIFISTIREIKAVKHPNEFRVFVHFLCVSVILALFLAA 240

Query: 241 LIFIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDRP 300
           L+FIQKR++ DQSAH A++AAI +LL LPLLIAIREE+VLWNL K T G +         
Sbjct: 241 LVFIQKRIRFDQSAHAAVVAAIASLLLLPLLIAIREEIVLWNLNKRTIGIH--------- 300

Query: 301 EPPTATTSSSSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSL 360
           +P T+    SSS             NKP RGED TI QAI S DM IL ++ML+GVG+SL
Sbjct: 301 QPQTSPNPPSSS-------------NKPSRGEDHTIPQAIFSADMLILCITMLIGVGASL 360

Query: 361 TAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLI 420
           TAIDNL QIGES GYP +TINS I LVSIFNFTGRIFSGFVS+ILLEK++FPRPLMLTL+
Sbjct: 361 TAIDNLRQIGESHGYPSETINSLIVLVSIFNFTGRIFSGFVSDILLEKFKFPRPLMLTLV 420

Query: 421 LFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLS 480
           L VSC+GHL+VAFPF DSLY+AS++IGFS+G+QVPL+FA+ISE+FGLKHYSTLFNFGQL 
Sbjct: 421 LLVSCIGHLLVAFPFNDSLYIASIVIGFSLGAQVPLHFAMISELFGLKHYSTLFNFGQLF 480

Query: 481 CPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLV 540
           CP+GSYILNV+VTG+LYDE+AKT      L C G  C+ QSF ILA LTF VA+++L+LV
Sbjct: 481 CPIGSYILNVMVTGRLYDEMAKTTTGIGGLHCEGHSCYEQSFVILAGLTFFVALVSLILV 540

Query: 541 MRTSEFYRGDIYKKFREDMDTLKTEVEFYRVDEKRTKIGNLFVDKHTINFK 592
            RT EFY+GDIYKKFREDM++LKT++EFY +DEKRT+IGNL VDKH+IN K
Sbjct: 541 ERTREFYKGDIYKKFREDMESLKTKMEFYTLDEKRTRIGNLLVDKHSINIK 569

BLAST of Moc03g21070 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 621.3 bits (1601), Expect = 7.9e-178
Identity = 320/561 (57.04%), Postives = 416/561 (74.15%), Query Frame = 0

Query: 13  IGSGEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGF 72
           +G+ E   F+     GRWF VFASFLIM+ AG  YLF  YSKDIKSTL  DQTTLN +GF
Sbjct: 1   MGNDETVRFLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGF 60

Query: 73  YKDLGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYFCFYIM 132
           +KDLG+NVGV++GL+AEV PTWF+L IG+A NF+GYF IW  VTGK+ +P V   C YI 
Sbjct: 61  FKDLGANVGVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYIC 120

Query: 133 IGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDTKSLIL 192
           IGANS NFANTG LVTCV+NFPE RGVMLGLLKG+VGLSGAI TQLY AIYGHD+KSLIL
Sbjct: 121 IGANSQNFANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLIL 180

Query: 193 LIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKRVQLDQ 252
           LI W P+ +SL F+Y IRE +  +  NE  VF QFL +++ LA+FL  +   +K+V   +
Sbjct: 181 LIAWLPAAVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSK 240

Query: 253 SAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDRPEPPTATTSSSSS 312
           +A+ A      ALLF+PL +++++EL +WN+ K       + ++V++P+         ++
Sbjct: 241 AAYAASATICCALLFVPLTVSVKQELEVWNMMKLPIEEP-SEVKVEKPKKELDLDQDKAA 300

Query: 313 ----VQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAIDNLGQ 372
                + ++ SCF+ +F+ P RGED+TILQA+LS DM ILF++   G+GSSLTA+DNLGQ
Sbjct: 301 KVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQ 360

Query: 373 IGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFVSCVGH 432
           IGESLGYP+ T++SF+SLVSI+N+ GR+FSGFVSE LL KY+ PRPLM+TL+L +SC GH
Sbjct: 361 IGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGH 420

Query: 433 LVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPVGSYIL 492
           L++AFP   S+Y+AS+L+GFS G+Q+PL FA+ISE+FGLK+YSTLFN GQL+ P+GSYIL
Sbjct: 421 LLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYIL 480

Query: 493 NVLVTGKLYDEVA--------KTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLV 552
           NV VTG LYD+ A         T  D K L C G  C++  F ILAA+TF  A+++L L 
Sbjct: 481 NVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLA 540

Query: 553 MRTSEFYRGDIYKKFREDMDT 562
           +RT EFY+GDIYKKFRE  ++
Sbjct: 541 IRTREFYKGDIYKKFRESPES 560

BLAST of Moc03g21070 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 570.1 bits (1468), Expect = 2.1e-162
Identity = 301/558 (53.94%), Postives = 391/558 (70.07%), Query Frame = 0

Query: 16  GEMSSFIRQVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGFYKD 75
           G M S   Q++ GRWF  F S LIMS AG  Y+F  YS DIK TL  DQTTLN + F+KD
Sbjct: 8   GSMKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKD 67

Query: 76  LGSNVGVIAGLLAEVVPTWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYFCFYIMIGA 135
           LG+NVGV+AGLL EV P WF+LLIGA  NF GYF IW  VT +I +P V + C YI +GA
Sbjct: 68  LGANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGA 127

Query: 136 NSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDTKSLILLIG 195
           NS +FANTG LVTCV+NFPE RGV+LG+LKG+VGLSGAI+TQLY A YG DTK LIL+IG
Sbjct: 128 NSQSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIG 187

Query: 196 WFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKRVQLDQSAH 255
           W P+++S  F+ TIR ++ ++  NE +VF  FL +++ LA FL V+I I K     QS  
Sbjct: 188 WLPAIVSFAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 247

Query: 256 IAILAAIFALLFLPLLIAIREELVLWNLKKTTTG-----NYFT-RIRVDRPEPPTATTSS 315
               A +  LL LP+++ I EE  LW  K+         N  T + ++D  E        
Sbjct: 248 GGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALNDPAPINVVTEKPKLDSSEFKDDDGEE 307

Query: 316 SSSV--QTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAIDNLG 375
           S  V  + ++ SC+  +FN PERG+D+TILQA+ SVDM ILF++ + GVG +LTAIDNLG
Sbjct: 308 SKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLG 367

Query: 376 QIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFVSCVG 435
           QIG SLGYP +++++F+SLVSI+N+ GR+ SG VSEI L KY+FPRPLMLT++L +SC G
Sbjct: 368 QIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAG 427

Query: 436 HLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPVGSYI 495
           HL++AF     LY+ASV+IGF  G+Q PL FA+ISEIFGLK+YSTL+NFG ++ P+GSY+
Sbjct: 428 HLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYL 487

Query: 496 LNVLVTGKLYD--------EVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVL 555
           LNV V G LYD         + KT V+ + L C G  CF+ SF I+AA+T    ++++VL
Sbjct: 488 LNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVL 547

Query: 556 VMRTSEFYRGDIYKKFRE 558
           V+RT +FY+ DIYKKFRE
Sbjct: 548 VIRTKKFYKSDIYKKFRE 565

BLAST of Moc03g21070 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 317.4 bits (812), Expect = 2.5e-86
Identity = 201/554 (36.28%), Postives = 304/554 (54.87%), Query Frame = 0

Query: 24  QVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGFYKDLGSNVGVI 83
           +++  +W ++ AS  I   +G  Y F  YS  +KST   DQ+TL+ +  +KD+G+N GV 
Sbjct: 2   EILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGVF 61

Query: 84  AGLLAEVVPT----------------WFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYF 143
           +GLL     +                W +L +GA   F GYF IW  VTG I +P V   
Sbjct: 62  SGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPLM 121

Query: 144 CFYIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDT 203
           C ++ + A S  F NT  +V+ V NF +  G  +G++KGF+GLSGAI+ QLY  +   D 
Sbjct: 122 CLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGDP 181

Query: 204 KSLILLIGWFPSLLSLFF-----IYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLI 263
            S ILL+   P++LSL       IY       +KH N          V++I+A +L ++I
Sbjct: 182 ASFILLLAVTPTVLSLLVMPLVRIYETSVADDKKHLNGLS------AVSLIIAAYLMIII 241

Query: 264 FIQKRVQLDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDRPEP 323
            ++    L   A+I  L  +  +L LPLLIA R +     ++KT   +Y   I       
Sbjct: 242 ILKNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRD--GMEKTVPHDYSPLI-----SS 301

Query: 324 PTATTSSSSSVQTQSSSCFANIFNKPERG--EDFTILQAILSVDMFILFMSMLVGVGSSL 383
           P ATTS + S +  S         K E G  E+  +LQA+  +  ++LF++M+ G+GS L
Sbjct: 302 PKATTSGNQSSEGDS---------KVEAGLSENLNLLQAMKKLSFWLLFLAMICGMGSGL 361

Query: 384 TAIDNLGQIGESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLI 443
           + I+N+ QIGESL Y    INS +SL SI+NF GR  +G+ S+ LL K  +PRPL++   
Sbjct: 362 STINNIRQIGESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAAT 421

Query: 444 LFVSCVGHLVVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLS 503
           L    +GHL++A  F+ +LY+ SV++G   GSQ  L   + SE+FG++H  T+FN   ++
Sbjct: 422 LGTMSIGHLIIASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVA 481

Query: 504 CPVGSYILNVLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLV 555
            P+GSYI +V + G +YD+ A      +   C+G HCFR SF I+A++ F   ++ +VL 
Sbjct: 482 SPIGSYIFSVRLIGYIYDKTA----SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLF 529

BLAST of Moc03g21070 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 310.1 bits (793), Expect = 3.9e-84
Identity = 192/545 (35.23%), Postives = 307/545 (56.33%), Query Frame = 0

Query: 24  QVVVGRWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGFYKDLGSNVGVI 83
           +++  +W ++ AS  I   AGG Y F  YS  +KST   DQ+TL+ +  +KD+G NVGV+
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 84  AGLLAEVVP--------------TWFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYFCF 143
           +GL+                    W ++LIGA  NF GYF +W  VTG I RP V   C 
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 144 YIMIGANSINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDTKS 203
           ++ I A S+ F NT  +V+ + NF +  G  +G++KGFVGLSGA++ QLY  +   D K+
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 204 LILLIGWFPSLLSLFFIYTIRELRAEKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKRVQ 263
            ILL+   PSLLS+  +  +R  +     +E +       +++I+A +L + I ++  + 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKTST-VDEKKHLDGLSTLSLIIAAYLMITIILKSTLS 243

Query: 264 LDQSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDRPEPPTATTSS 323
           L   A+   LA +  LL  PLL+A+R      +++K  +  Y     VD  E   ATTS 
Sbjct: 244 LPSWANAVTLAVLLVLLSSPLLVAVRAHRD--SIEKPLSSVYSP--LVDNLE---ATTSG 303

Query: 324 SSSVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAIDNLGQI 383
              +  +  S                +LQA+ +VD ++LF++M+ G+GS ++ I+N+ QI
Sbjct: 304 EILMLDEDKS--------------LNLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQI 363

Query: 384 GESLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFVSCVGHL 443
           GESL Y    INS ++L +I+NF GR   G+VS+ LL +  +PRPL++   L    +GHL
Sbjct: 364 GESLRYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHL 423

Query: 444 VVAFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPVGSYILN 503
           ++A  F+ +LY  S+++G   GSQ  L   + SE+FG+KH  T++N   ++ P+GSYI +
Sbjct: 424 IIASGFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFS 483

Query: 504 VLVTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLVMRTSEFYRG 555
           V + G +YD      +  +   C+G HCFR ++ ++A++ F+  +++ VLV RT   YR 
Sbjct: 484 VRLIGYIYDRT----IIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKTIYR- 521

BLAST of Moc03g21070 vs. TAIR 10
Match: AT2G34350.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 297.0 bits (759), Expect = 3.4e-80
Identity = 185/543 (34.07%), Postives = 294/543 (54.14%), Query Frame = 0

Query: 29  RWFSVFASFLIMSGAGGVYLFAYYSKDIKSTLRCDQTTLNKIGFYKDLGSNVGVIAGLLA 88
           +W +  AS  I S +G  Y F  YS  +KS+   DQ+TL+ +  YKD+G+NVG+++GL  
Sbjct: 7   KWVAAAASIWIQSFSGASYTFGIYSSVLKSSQSYDQSTLDTVSVYKDIGANVGILSGLFY 66

Query: 89  EVVPT------------WFLLLIGAAFNFLGYFKIWQGVTGKIVRPTVGYFCFYIMIGAN 148
             V +            W ++ +G    F+GY  IW   +G I RP V   C ++    +
Sbjct: 67  TAVASRKSGNGGFFSGPWLVIFVGLLQWFVGYGFIWMATSGVIPRPPVAMMCLFMFFAGH 126

Query: 149 SINFANTGVLVTCVRNFPERRGVMLGLLKGFVGLSGAIMTQLYVAIYGHDTKSLILLIGW 208
              F NT ++VT VRNF +  G  +G++KG++GLSGAI+ Q+Y    G D ++ ILL+  
Sbjct: 127 CQPFFNTAIVVTAVRNFSDYGGTAVGIMKGYLGLSGAILVQMYHIFCGGDPRNYILLLAV 186

Query: 209 FPSLLSLFFIYTIRELRA-----EKHPNEFRVFVQFLCVTVILAVFLTVLIFIQKRVQLD 268
            PSLL L  +  +R         +KH N          +++I+  +L V+I ++  + + 
Sbjct: 187 VPSLLILTLMPFVRTYDTVIAGDKKHLNGLS------AISLIIVTYLMVVILVENIIGMS 246

Query: 269 QSAHIAILAAIFALLFLPLLIAIREELVLWNLKKTTTGNYFTRIRVDRPEPPTATTSSSS 328
               I     +  LL  PLL+A+R +            + F  +      P T  T+   
Sbjct: 247 MPMKICSFTFLLLLLASPLLVAVRAQ--------REEEHRFLSLDF----PVTERTTLLD 306

Query: 329 SVQTQSSSCFANIFNKPERGEDFTILQAILSVDMFILFMSMLVGVGSSLTAIDNLGQIGE 388
           S +  SSS   ++        D  +L+AI + + ++LF++M+ G+GS L  I+N+ Q+GE
Sbjct: 307 SPKLNSSSDVKDVMT-----NDMNVLEAICTTNFWLLFVAMICGMGSGLATINNIRQMGE 366

Query: 389 SLGYPHQTINSFISLVSIFNFTGRIFSGFVSEILLEKYQFPRPLMLTLILFVSCVGHLVV 448
           SL Y    +NS +SL SI+NF GR  SG++S+  L  + +PRP+ + + L +  +GH+V+
Sbjct: 367 SLRYSTVQLNSLVSLWSIWNFLGRFGSGYISDTYLHSHGWPRPVFMAITLGLMAIGHIVM 426

Query: 449 AFPFEDSLYLASVLIGFSMGSQVPLYFAVISEIFGLKHYSTLFNFGQLSCPVGSYILNVL 508
           A     SLY+ S+L+G + GSQ  L   + SEIFG+ H  T+F    ++ PVGSY  +V 
Sbjct: 427 ASGLLGSLYIGSLLVGLAYGSQWSLMPTITSEIFGVLHMGTIFYTISIASPVGSYFFSVK 486

Query: 509 VTGKLYDEVAKTGVDPKALQCHGVHCFRQSFTILAALTFVVAVLTLVLVMRTSEFYRGDI 555
           V G LYD+VA          C+G HCFR SF I+AA+  + +++ LVL++RT +FY   +
Sbjct: 487 VIGYLYDKVASEDDH----SCYGNHCFRTSFLIMAAMALLGSLVALVLLLRTKKFYATLV 522

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038895750.13.7e-27078.17uncharacterized protein LOC120083915 [Benincasa hispida][more]
XP_008464809.16.8e-26477.52PREDICTED: uncharacterized protein LOC103502605 [Cucumis melo][more]
KAA0051898.19.8e-26377.93protein NUCLEAR FUSION DEFECTIVE 4 [Cucumis melo var. makuwa] >TYJ99888.1 protei... [more]
XP_004147303.32.9e-26275.83uncharacterized protein LOC101202941 [Cucumis sativus] >KGN64782.2 hypothetical ... [more]
XP_022998867.18.3e-25476.09uncharacterized protein LOC111493395 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
F4I9E14.3e-2723.46Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A1S3CMG93.3e-26477.52uncharacterized protein LOC103502605 OS=Cucumis melo OX=3656 GN=LOC103502605 PE=... [more]
A0A5D3BP484.7e-26377.93Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A6J1K9654.0e-25476.09uncharacterized protein LOC111493395 OS=Cucurbita maxima OX=3661 GN=LOC111493395... [more]
A0A6J1GAT54.0e-25476.25uncharacterized protein LOC111452462 OS=Cucurbita moschata OX=3662 GN=LOC1114524... [more]
A0A6J1HLR33.5e-24272.93uncharacterized protein LOC111464761 OS=Cucurbita moschata OX=3662 GN=LOC1114647... [more]
Match NameE-valueIdentityDescription
AT2G28120.17.9e-17857.04Major facilitator superfamily protein [more]
AT2G39210.12.1e-16253.94Major facilitator superfamily protein [more]
AT1G74780.12.5e-8636.28Nodulin-like / Major Facilitator Superfamily protein [more]
AT1G18940.13.9e-8435.23Nodulin-like / Major Facilitator Superfamily protein [more]
AT2G34350.13.4e-8034.07Nodulin-like / Major Facilitator Superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 552..572
NoneNo IPR availablePANTHERPTHR21576UNCHARACTERIZED NODULIN-LIKE PROTEINcoord: 14..563
NoneNo IPR availablePANTHERPTHR21576:SF29PROTEIN NUCLEAR FUSION DEFECTIVE 4coord: 14..563
NoneNo IPR availableCDDcd17354MFS_Mch1p_likecoord: 28..530
e-value: 9.90266E-94
score: 291.077
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 338..547
e-value: 4.2E-12
score: 47.9
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 256..544
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 53..270
IPR010658Nodulin-likePFAMPF06813Nodulin-likecoord: 29..274
e-value: 4.0E-79
score: 265.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g21070.1Moc03g21070.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0022857 transmembrane transporter activity