Moc03g20880 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g20880 vs. NCBI nr
Match: XP_020080565.1 (uncharacterized protein LOC109704209 [Ananas comosus])

HSP 1 Score: 157.5 bits (397), Expect = 1.7e-34
Identity = 94/222 (42.34%), Postives = 131/222 (59.01%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK   H LTD Q  +  IRSL    EHMKV+ T ++ IK   DV  ++  +++R+ A
Sbjct: 63  IRELKSAGHTLTDVQQGQAVIRSLPDSWEHMKVNLTYNDNIKTFADVARYVELEDERLGA 122

Query: 79  MRLDNVETYVAGPSSYGNNGYKRKHQEVFKGKRDHRIKKRKQDSGATDHVAKDRGVYVKF 138
            R D V  Y+   SS+  +G       +        I     DSGAT H+A DRG +V++
Sbjct: 123 TR-DPVHAYITESSSHKASG---SGMVLLIDSYSLWI----IDSGATYHIAGDRGAFVEY 182

Query: 139 RRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLSVTVLFKL 198
           RR+ +  +W+YVG NA+V VK IG+CK+ +R   TLL HDVLYAP+I RNL+SV+VL  L
Sbjct: 183 RRVQAGTKWIYVGNNAKVDVKRIGTCKVVLRGRRTLLFHDVLYAPEIRRNLVSVSVLLGL 242

Query: 199 GYTFTLTENSLTLSLNNIHYGYGFIFNGFIVLDVVETISYNY 241
           GY      N + +   +++YG G++ NGF VLD    + YNY
Sbjct: 243 GYGLNFCNNRVDIFYESVYYGTGYLLNGFFVLDCEHNV-YNY 275

BLAST of Moc03g20880 vs. NCBI nr
Match: XP_022889045.1 (uncharacterized protein LOC111404476 [Olea europaea var. sylvestris])

HSP 1 Score: 153.7 bits (387), Expect = 2.5e-33
Identity = 99/268 (36.94%), Postives = 147/268 (54.85%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK   H+L+DEQ V+  IRSL Y+ EH+KV+ T ++ IK   D  CH+  +++R+ A
Sbjct: 42  IRELKLVGHILSDEQQVQASIRSLFYNWEHLKVNLTHNDSIKTFADAACHVELEDERLGA 101

Query: 79  MRLDNVETYVAGPSSYGNNGYKRKHQEVFKG-----------------KRDHRIK----- 138
            +   V+ +VA  SS   +G+KRK +  FKG                 KR+++ K     
Sbjct: 102 AKF-LVQAFVAESSSGKASGFKRK-KNWFKGGKGKGKETRQGPKKNKHKRNNKGKWFGKK 161

Query: 139 ------------------------KRKQDSGATDHVAKDRGVYVKFRRIPSRARWLYVGT 198
                                   K+  DSGA DHV +DR V+V+F R P+  +W+YVG 
Sbjct: 162 KDKSKLKYFNYQVMGHFAQECPELKKIVDSGAIDHVTRDREVFVEFCRFPASTKWIYVGN 221

Query: 199 NARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLSVTVLFKLGYTFTLTENSLTLS 241
           + RV VK IG+CKL++    T+LLHDVL+A  I RNL+ V VL +L +      NS+ ++
Sbjct: 222 DDRVEVKGIGTCKLHLHGGRTILLHDVLFAQGIRRNLIFVVVLLRLRFNLNFCGNSVRIN 281

BLAST of Moc03g20880 vs. NCBI nr
Match: KAG7548157.1 (Integrase catalytic core [Arabidopsis suecica])

HSP 1 Score: 146.4 bits (368), Expect = 4.0e-31
Identity = 93/281 (33.10%), Postives = 142/281 (50.53%), Query Frame = 0

Query: 28  VLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEAMRLDNVETY 87
           +++DEQ V+  +RSL    +HMK+  T ++ +K   D++ +L  +++R+EA R  N   Y
Sbjct: 116 IMSDEQQVQAVLRSLPESWDHMKIQMTHNDNVKTFEDISRYLELEDERLEAARPFNEANY 175

Query: 88  VAGPS-------SYGNNGY-----KRKHQEVFKGKRDHRIKKRKQ--------------- 147
            +  S       ++ N        K++ +   +GK+  R  K K                
Sbjct: 176 ASSSSGMKRKRGNFDNKASEQANPKKQKKHAKRGKKGGRKDKTKMKCYNCDTLGHFAHEC 235

Query: 148 ---------------------------------DSGATDHVAKDRGVYVKFRRIPSRARW 207
                                            DSGATDHVA+ R  + ++RRIP   RW
Sbjct: 236 TEAKKVLSLKNSRKYAYVSSCVMLAESNPLWIVDSGATDHVARSREAFTEYRRIPKGTRW 295

Query: 208 LYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLSVTVLFKLGYTFTLTEN 248
           LYVG N +V V  IG+C+L+MR   TL+LHDVLYAP+I R+L+SV  L +LG++    + 
Sbjct: 296 LYVGNNTKVAVIGIGTCQLHMRGGKTLILHDVLYAPEIRRDLVSVLALLRLGFSLNFYDM 355

BLAST of Moc03g20880 vs. NCBI nr
Match: KAG7595359.1 (Reverse transcriptase RNA-dependent DNA polymerase [Arabidopsis thaliana x Arabidopsis arenosa])

HSP 1 Score: 142.9 bits (359), Expect = 4.4e-30
Identity = 99/290 (34.14%), Postives = 146/290 (50.34%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK    +++DEQ ++  +RSL    +HM+V  T +  +K   D++ HL  +++R+EA
Sbjct: 99  IRELKNAGQIVSDEQQIQAVLRSLPESWDHMRVQMTHNVNVKTFEDISRHLELEDERLEA 158

Query: 79  MR---LDNVETYVAG-PSSYGNNGYKRKHQEVFK-----GKRDHRIKKRKQ--------- 138
            +     NV + ++G     GN G K  +Q   K      KR  R  K+ +         
Sbjct: 159 AKPFNEANVASSISGLKRKRGNKGNKAPYQTGLKKQNKFAKRGKRGGKKDKTNVKCYNCG 218

Query: 139 -----------------------------------------DSGATDHVAKDRGVYVKFR 198
                                                    DSGATDHVA+DR  +V++R
Sbjct: 219 LLGHFARECTEAKKVSLNSTCKYAYVSSCVMLAESHPLWIVDSGATDHVARDREAFVEYR 278

Query: 199 RIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLSVTVLFKLG 248
           RIP  +RWLYVG N++V V  IG+C+L+MR   TL+LHDVL AP+I R+L+SV  L KLG
Sbjct: 279 RIPKGSRWLYVGNNSKVAVLGIGTCQLHMRGGRTLILHDVLNAPEIRRDLVSVLALLKLG 338

BLAST of Moc03g20880 vs. NCBI nr
Match: TXG60589.1 (hypothetical protein EZV62_015162 [Acer yangbiense])

HSP 1 Score: 141.4 bits (355), Expect = 1.3e-29
Identity = 85/209 (40.67%), Postives = 122/209 (58.37%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK   H L DEQ ++  IRSL    E+MK++ T +  IK  +D++ H+  +++R+EA
Sbjct: 67  IRELKSAGHTLIDEQQIQAVIRSLPNSWENMKINMTHN--IKTFDDISRHVELEDERLEA 126

Query: 79  MRLDNVETYVAGPSSYGNNGYKRKHQE-------VFKGKRDHRIKKRKQDSGATDHVAKD 138
            +    + Y+A  S     G+K   ++         KGK    I+  K++SGATDHVA+D
Sbjct: 127 AKASG-QLYMAESSKRKTKGFKHNGKKGNFQKGNSQKGKWKGPIENFKKNSGATDHVARD 186

Query: 139 RGVYVKFRRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLS 198
           R  +V++ RI    RW+YV  N+RV VK IG+CKL M +   L LHDVLYAP I RNL+S
Sbjct: 187 RAAFVEYHRISQGTRWIYVENNSRVEVKGIGTCKLMMHSGQVLYLHDVLYAPGIRRNLVS 246

Query: 199 VTVLFKLGYTFTLTENSLTLSLNNIHYGY 221
           V VL  LGY        + + LN++  G+
Sbjct: 247 VIVLLGLGYELNFYGVCMDIVLNSVCVGF 272

BLAST of Moc03g20880 vs. ExPASy TrEMBL
Match: A0A6P5EGH5 (uncharacterized protein LOC109704209 OS=Ananas comosus OX=4615 GN=LOC109704209 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 8.3e-35
Identity = 94/222 (42.34%), Postives = 131/222 (59.01%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK   H LTD Q  +  IRSL    EHMKV+ T ++ IK   DV  ++  +++R+ A
Sbjct: 63  IRELKSAGHTLTDVQQGQAVIRSLPDSWEHMKVNLTYNDNIKTFADVARYVELEDERLGA 122

Query: 79  MRLDNVETYVAGPSSYGNNGYKRKHQEVFKGKRDHRIKKRKQDSGATDHVAKDRGVYVKF 138
            R D V  Y+   SS+  +G       +        I     DSGAT H+A DRG +V++
Sbjct: 123 TR-DPVHAYITESSSHKASG---SGMVLLIDSYSLWI----IDSGATYHIAGDRGAFVEY 182

Query: 139 RRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLSVTVLFKL 198
           RR+ +  +W+YVG NA+V VK IG+CK+ +R   TLL HDVLYAP+I RNL+SV+VL  L
Sbjct: 183 RRVQAGTKWIYVGNNAKVDVKRIGTCKVVLRGRRTLLFHDVLYAPEIRRNLVSVSVLLGL 242

Query: 199 GYTFTLTENSLTLSLNNIHYGYGFIFNGFIVLDVVETISYNY 241
           GY      N + +   +++YG G++ NGF VLD    + YNY
Sbjct: 243 GYGLNFCNNRVDIFYESVYYGTGYLLNGFFVLDCEHNV-YNY 275

BLAST of Moc03g20880 vs. ExPASy TrEMBL
Match: A0A5C7HUX5 (Uncharacterized protein OS=Acer yangbiense OX=1000413 GN=EZV62_015162 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 6.2e-30
Identity = 85/209 (40.67%), Postives = 122/209 (58.37%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK   H L DEQ ++  IRSL    E+MK++ T +  IK  +D++ H+  +++R+EA
Sbjct: 67  IRELKSAGHTLIDEQQIQAVIRSLPNSWENMKINMTHN--IKTFDDISRHVELEDERLEA 126

Query: 79  MRLDNVETYVAGPSSYGNNGYKRKHQE-------VFKGKRDHRIKKRKQDSGATDHVAKD 138
            +    + Y+A  S     G+K   ++         KGK    I+  K++SGATDHVA+D
Sbjct: 127 AKASG-QLYMAESSKRKTKGFKHNGKKGNFQKGNSQKGKWKGPIENFKKNSGATDHVARD 186

Query: 139 RGVYVKFRRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLS 198
           R  +V++ RI    RW+YV  N+RV VK IG+CKL M +   L LHDVLYAP I RNL+S
Sbjct: 187 RAAFVEYHRISQGTRWIYVENNSRVEVKGIGTCKLMMHSGQVLYLHDVLYAPGIRRNLVS 246

Query: 199 VTVLFKLGYTFTLTENSLTLSLNNIHYGY 221
           V VL  LGY        + + LN++  G+
Sbjct: 247 VIVLLGLGYELNFYGVCMDIVLNSVCVGF 272

BLAST of Moc03g20880 vs. ExPASy TrEMBL
Match: A0A151QKT8 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cajanus cajan OX=3821 GN=KK1_049743 PE=4 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 1.4e-29
Identity = 93/284 (32.75%), Postives = 133/284 (46.83%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELKE  H L+DEQ V+  IRSL    EHMKV+ T +E I   +DV  HL  +++R+EA
Sbjct: 63  IRELKEAGHTLSDEQQVQAVIRSLPGSWEHMKVNLTHNENITTFSDVAKHLELEDERLEA 122

Query: 79  MR-------LDNVETYVAGPSSYGNNGYKRKHQ----EVFKGKRDHRIKKRKQ------- 138
            +        +   T  A P+S     ++RK +    +  KGK+  + K  K        
Sbjct: 123 YKSSGQAYYTEAASTSQAKPNSKKGKFFQRKGKKGGFQPKKGKKQFKKKGNKGGKKKIDL 182

Query: 139 -------------------------------------------------------DSGAT 198
                                                                  DSG+T
Sbjct: 183 KKVKCYACQKMGHFARDCSKQTESEKVLVNYVNSHVSYVSSTVFLTEPISFSWIIDSGST 242

Query: 199 DHVAKDRGVYVKFRRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDI 230
           DHV +DRG ++++RR+P+ +RWLYVG NAR+ VK +G+C+  +R   TL LHDVLY PDI
Sbjct: 243 DHVTRDRGSFIEYRRLPNHSRWLYVGNNARIEVKGLGTCQFTLRGGKTLTLHDVLYTPDI 302

BLAST of Moc03g20880 vs. ExPASy TrEMBL
Match: A0A2U1LF40 (Uncharacterized protein OS=Artemisia annua OX=35608 GN=CTI12_AA497560 PE=4 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 7.6e-28
Identity = 92/253 (36.36%), Postives = 131/253 (51.78%), Query Frame = 0

Query: 6   IRDRLIFKLDTIAISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDV 65
           +R  LI   + IA  EL      LTDE+ V+  I+SL+     MK++ T +  IK  +DV
Sbjct: 56  MRQHLIIMSNMIA--ELNTTGRELTDEEEVQAVIKSLTKKWNDMKLNLTHNGNIKTFDDV 115

Query: 66  TCHLGWKEDRMEAMRLDNVETYVAGPSSYGNNGYKRKHQEVFKGKRDHRIKKRKQDSGAT 125
           + HL  +EDR+ + + D +E YVA  S      +KR      KGK            G T
Sbjct: 116 SRHLELEEDRLGSEQND-IEAYVAESSWRKKFNFKRN-----KGK------------GVT 175

Query: 126 DHVAKDRGVYVKFRRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDI 185
            H+ +DR  +V +RRIP  ++++Y+  + +     I +CKL MR   TL LHDVLYAPD+
Sbjct: 176 YHITRDRAAFVDYRRIPRGSKYIYIRNDTKAEAFGIATCKLKMRGGRTLYLHDVLYAPDV 235

Query: 186 WRNLLSVTVLFKLGYTFTLTENSLTLSLNNIHYGYGFIFNGFIVLDVV-ETISYNYASFS 245
            R+L+SV VL  LGY     +  + L L   +YG G I +GF+VLD V      N   FS
Sbjct: 236 RRSLVSVLVLLNLGYKLVFDDRCVDLYLGITYYGTGHIMDGFMVLDTVNNNCKVNTNCFS 288

Query: 246 LITEGIGSRSRSA 258
            +     + S  A
Sbjct: 296 YVASSSSNVSDDA 288

BLAST of Moc03g20880 vs. ExPASy TrEMBL
Match: A0A6D2J7K8 (Uncharacterized protein OS=Microthlaspi erraticum OX=1685480 GN=MERR_LOCUS22816 PE=4 SV=1)

HSP 1 Score: 133.7 bits (335), Expect = 1.3e-27
Identity = 87/265 (32.83%), Postives = 130/265 (49.06%), Query Frame = 0

Query: 19  ISELKEPDHVLTDEQHVKPDIRSLSYDEEHMKVHFTRDEVIKPLNDVTCHLGWKEDRMEA 78
           I ELK    +++DEQ V+  +RSL    +HM++  T +  +K   D++ HL  +++R+EA
Sbjct: 153 IRELKNAGQIMSDEQQVQAVLRSLPESWDHMRMQMTHNVNVKTFEDISRHLELEDERLEA 212

Query: 79  MRLDNVETYVAGPSSY----GNNGY--------KRKHQEVFKGKRDHRIKKRK------- 138
            +  N   Y    S      GNNG         KR+ + V +GKR  +  K K       
Sbjct: 213 AKPFNEANYANSNSGLKRKRGNNGNKAPDQPDPKRQKKYVKRGKRGGKKDKTKIKCYNCG 272

Query: 139 -----------------------------------------QDSGATDHVAKDRGVYVKF 198
                                                     DSGATDHVA+ R  +V++
Sbjct: 273 SLGHFARECTEAKKVLSLDSTCKYAYVSSCVMLTESHPLWIVDSGATDHVARSREAFVEY 332

Query: 199 RRIPSRARWLYVGTNARVPVKDIGSCKLNMRNECTLLLHDVLYAPDIWRNLLSVTVLFKL 224
           RR+P   RWLYVG N++V V+ IG+C+L+M    TL+LHDVLYAP+I  +L+SV  L KL
Sbjct: 333 RRVPKGTRWLYVGNNSKVAVQGIGTCQLHMSGGKTLILHDVLYAPEIRGDLVSVLALLKL 392