Moc03g03390 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc03g03390
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Methylsterol monooxygenase 2-1
Location	chr3: 2546775 .. 2549550 (-)
RNA-Seq Expression	Moc03g03390
Synteny	Moc03g03390

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCGATTCTTGAATCTGCCTGGCTGGTACTGTTTCTGCTTCTTCTTCTTCTTCTTTCCGCCCTACATTTCATCTTCTACTTGTTAATTTGTCGCTGACGCGAGAGTCACACTGGGGTCTAGATGTAATTTTGGGATTAATTGCATCTTTCACAGTTGATCCGAAACAGGATCTTGAAGTTGTACACTGGGGTTCGGATTTTTTACTTTTTAATTGGAGTTGGAGGACCGAATTTGGGTTTCGTCAGAATATGGGTATTGCTGGAGTTTTGAATTACACTATGATCCTTTCTAAATCTGATACTTGTTTTATGGAACTTTTTCCCCACTATGCAGTATCTAATCACAAATTTCAGTGATTTTCAACTGGCTTCTATTGGAACCTTCATAGTCCATGAAAGCGTCTTCTTCTTATTTGGGCTTCCATTTATTTTTCTGGAAAGAACAGGATGGCTGAGCAAGTACAAGATCCAGGTTTACTTCGTCTCTTCACCATATTTTTTTTTAGCCAAGGCTTCATTTCATAACTGCAAGTTCTTATGGATGTTTTTTTTTTTTTTTTTTTTTTAAATTTAGATTTGCCTTCATTAGCTACAGCAGTACTTTAACTAGTATTTTAATAATCCTTTTATTTTATCTGAAACAAAGGAAATAACAGGAAATAATGAGAGGTTTAAACTTTAAACTATGTTTCCCATTAATTATCCTTTGTATCAAGAAGAAAAGAAGGGGGGAGAAAAAAAGAACTGTTTCTCATTGATTACCATGTTTACAATAATGCTGATGCTTCAAGATGCAGTATTAACTACCTATCAGCTAAGTTTTCTACCATACTTAAGAACCAGGCTGAACTTTCAGCACAATAATTATAGCTTCACTAACTCAATAGAGGCCTGACTAACTCTTAATTCTTAATGAATTAGTTTTCAGTTGAGATATGAATTTTTTCACCTTTGTGCAGGCAAAGACTAATAGTCCTGCAGCTCAAGGAAAATGCATTATTCGCCTACTTATGTATCATTGTGGTGTAAATCTGCCACTTATGATTGTTTCATATCCCGTCTTCAAGCAAATGGGCATGAGAAGCAGTCTTCCATTGCCATCCTGGTATCTTCAGGTTCTTTATCTCCTAAAGCATATTTTAGATGTTCTACTTCTATCTTGCTAGGTTTAGCTTACTTTCACCATTTCCTTATCTTGGACCATAACATTAGCCAGTGCCTTCTGACCGAATCTAACTTTTTAGATGGATTTAACTTAGATCGGTAGAGTATTTTTTTTCAAAAGTAGAATTGATAGAATCTAATTTGGTTCGGGTTGAATCTAATTTGGTTCGGGTTTCATTAATGTAGGAAAGTTGTCTTCGGCCAGATACTATTTTACTTTATTGTGGAGGATTTTGTTTTCTACTGGGGACATAGAATTTTGCACACCAAATGGCTGTACAAGAATGTGCATAGCGTGCATCATGAGTAAGTGAAATCTATAATTGAAAATTAATTGAGATTCTGCCTTTTCCCATGATAAAATTGTCTTAGGCTATTTAAGTTGCTTATAAGTGCAGATATGCTACACCTTTTGGACTAACAGCGGAATACGCTCACCCTGCTGAGATTCTGTTCCTTGGTTTTGCTACTGTCATCGGTCCTGCCATTACTGGTCCCCATCTAATGACTCTGTGGTTATGGATGACACTTAGACTGATGGAGACAGTGGAGGCTCATTGTGGTTACCATTTTCCTTGGAGCCCTTCAAACTTCTTACCTTTATATGGGGGGTGAGTCAATTAGGATTTGTAATGTTTTAAAGAGCGTATTGTACTGTAGCTGTTGTTCCTGATATTGGCTTTTGCTCTTGCTCTGCAGTGCTTATTTTCATGATTATCATCATCGGTTGCTTTATACAAAATCTGGCAACTACTCATCAACATTCACTTACATGGACTGGTAGGTTCTGGTTCAAAATTGTCCAACCATTTATTTTGTATACATTATTGCTTCGAAATTAGTAGCATTAAAACCAGCTTTGTGGAACATTGATTTACTTCCGCTTCAATAATTTCACGCCTACTTTCTTAGATCATTGATCTACGTCGTAGTTTTTGAGACCCCCCCTAAACTGATCGCTGCATTTTTACTATGGTGGAGATATGTTAATCAGTGACTTGATGCTCTCACTTTAGGTTGAGATCATGTTAGGAATATTAGTAGGGAAACATTGAGGATATTATTAGGAGTGTTGGGAATATTAGTTGGGAAATATTAGAGATGTTATTAGGATATTTGGAGGGCATACTTTTAGCCTAATGTTTTCTACAATTGGATTACCTCCATTGAGTAAAAAAGGAAAGATAGATCTTTTTTTTTTAATTATTAAGAAAATTTGAATGTGGTGACTCTATGCTTGCTTTCAATTCGATCTTTCAACGAGCAACATGTTGTTACTATTTAGAAGTTTCATCTTCCTTGCATCTGTATCAACAGTCCCTACCATGACAAGTTACCAAATTTGATCATTTTGTGAATGGGGAACTTGGAGAGCCTTCAGATGAGAAAGTCATACCCTACCTATATGCATTTCATGTTTCTGCATGTGCTTTTGCTTCTTGTAATTGGTTATCATTTGTCCATGGGGTATCGATTGATAATGCGCTCTGCATGAAAATAGAAGACGAGCACTGACGAGGATGTTTCGGCTTCTTTGCTTTGGCAGGATATTTGGGACTGATAAGGGTTTCAGAAATTTGGAAGCTATAAAAAAGGCAGAAAATTGA

mRNA sequence

ATGGCTGCGATTCTTGAATCTGCCTGGCTGTATCTAATCACAAATTTCAGTGATTTTCAACTGGCTTCTATTGGAACCTTCATAGTCCATGAAAGCGTCTTCTTCTTATTTGGGCTTCCATTTATTTTTCTGGAAAGAACAGGATGGCTGAGCAAGTACAAGATCCAGGCAAAGACTAATAGTCCTGCAGCTCAAGGAAAATGCATTATTCGCCTACTTATGTATCATTGTGGTGTAAATCTGCCACTTATGATTGTTTCATATCCCGTCTTCAAGCAAATGGGCATGAGAAGCAGTCTTCCATTGCCATCCTGGAAAGTTGTCTTCGGCCAGATACTATTTTACTTTATTGTGGAGGATTTTGTTTTCTACTGGGGACATAGAATTTTGCACACCAAATGGCTGTACAAGAATGTGCATAGCGTGCATCATGAATATGCTACACCTTTTGGACTAACAGCGGAATACGCTCACCCTGCTGAGATTCTGTTCCTTGGTTTTGCTACTGTCATCGGTCCTGCCATTACTGGTCCCCATCTAATGACTCTGTGGTTATGGATGACACTTAGACTGATGGAGACAGTGGAGGCTCATTGTGGTTACCATTTTCCTTGGAGCCCTTCAAACTTCTTACCTTTATATGGGGGTGCTTATTTTCATGATTATCATCATCGGTTGCTTTATACAAAATCTGGCAACTACTCATCAACATTCACTTACATGGACTGGATATTTGGGACTGATAAGGGTTTCAGAAATTTGGAAGCTATAAAAAAGGCAGAAAATTGA

Coding sequence (CDS)

ATGGCTGCGATTCTTGAATCTGCCTGGCTGTATCTAATCACAAATTTCAGTGATTTTCAACTGGCTTCTATTGGAACCTTCATAGTCCATGAAAGCGTCTTCTTCTTATTTGGGCTTCCATTTATTTTTCTGGAAAGAACAGGATGGCTGAGCAAGTACAAGATCCAGGCAAAGACTAATAGTCCTGCAGCTCAAGGAAAATGCATTATTCGCCTACTTATGTATCATTGTGGTGTAAATCTGCCACTTATGATTGTTTCATATCCCGTCTTCAAGCAAATGGGCATGAGAAGCAGTCTTCCATTGCCATCCTGGAAAGTTGTCTTCGGCCAGATACTATTTTACTTTATTGTGGAGGATTTTGTTTTCTACTGGGGACATAGAATTTTGCACACCAAATGGCTGTACAAGAATGTGCATAGCGTGCATCATGAATATGCTACACCTTTTGGACTAACAGCGGAATACGCTCACCCTGCTGAGATTCTGTTCCTTGGTTTTGCTACTGTCATCGGTCCTGCCATTACTGGTCCCCATCTAATGACTCTGTGGTTATGGATGACACTTAGACTGATGGAGACAGTGGAGGCTCATTGTGGTTACCATTTTCCTTGGAGCCCTTCAAACTTCTTACCTTTATATGGGGGTGCTTATTTTCATGATTATCATCATCGGTTGCTTTATACAAAATCTGGCAACTACTCATCAACATTCACTTACATGGACTGGATATTTGGGACTGATAAGGGTTTCAGAAATTTGGAAGCTATAAAAAAGGCAGAAAATTGA

Protein sequence

MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTNSPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHLMTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKGFRNLEAIKKAEN

Homology

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BLAST of Moc03g03390 vs. NCBI nr
Match: XP_022158130.1 (methylsterol monooxygenase 2-2-like [Momordica charantia])

HSP 1 Score: 550.8 bits (1418), Expect = 6.3e-153
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN
Sbjct: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED
Sbjct: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAEN
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

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BLAST of Moc03g03390 vs. NCBI nr
Match: XP_008457442.1 (PREDICTED: methylsterol monooxygenase 2-2 [Cucumis melo])

HSP 1 Score: 510.4 bits (1313), Expect = 9.4e-141
Identity = 234/262 (89.31%), Postives = 252/262 (96.18%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAA+LESAW YLITNFSDFQLA IG+FI+HESVFFL GLPFI LER GWLSKYKIQAK N
Sbjct: 1   MAALLESAWQYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCI RLL+YH GVNLP+M+VSYPVFK+MGMRS+LPLPSWKVVFGQI+FYFI+ED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
            TLWLWM +R++ETVEAHCGYHFPWSPSNF+PLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 FTLWLWMVVRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAEN
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

[Close]

BLAST of Moc03g03390 vs. NCBI nr
Match: XP_022964698.1 (methylsterol monooxygenase 2-2 [Cucurbita moschata] >XP_022970560.1 methylsterol monooxygenase 2-2 isoform X2 [Cucurbita maxima] >XP_023519298.1 methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo] >XP_023521038.1 methylsterol monooxygenase 2-2-like [Cucurbita pepo subsp. pepo] >KAG6583405.1 Methylsterol monooxygenase 2-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7019171.1 Methylsterol monooxygenase 2-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 235/262 (89.69%), Postives = 252/262 (96.18%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MA +LESAW YLITNFSDFQLA IG+FI+HESVFFL GLPF+FLER GWLSKYKIQAK N
Sbjct: 1   MAELLESAWRYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCI RLL+YH GVNLP+MIVSYPVFK+MGMRS+LPLPSWKVVFGQI+FYFIVED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWM LR++ETVEAHCGY FPWSPSNF+PLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

[Close]

BLAST of Moc03g03390 vs. NCBI nr
Match: XP_022948631.1 (methylsterol monooxygenase 2-2-like [Cucurbita moschata] >KAG6607270.1 Methylsterol monooxygenase 2-2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036949.1 Methylsterol monooxygenase 2-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 508.1 bits (1307), Expect = 4.6e-140
Identity = 234/262 (89.31%), Postives = 253/262 (96.56%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAA+LESAW YLITNFSDFQLA +G+FI+HESVFFL GLPFIFLE+ GWLSKYKIQAKTN
Sbjct: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           S AAQGKCI RLL+YH GVNLP+MIVSYPVFK MGMRS+LPLPSWKVVFGQI+FYFIVED
Sbjct: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWM LR++ETVEAHCGYHFPWSPSNF+PLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

[Close]

BLAST of Moc03g03390 vs. NCBI nr
Match: XP_022998327.1 (methylsterol monooxygenase 2-2-like [Cucurbita maxima])

HSP 1 Score: 506.9 bits (1304), Expect = 1.0e-139
Identity = 234/262 (89.31%), Postives = 252/262 (96.18%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAA+LESAW YLITNFSDFQLA  G+FI+HESVFFL GLPFIFLE+ GWLSKYKIQAKTN
Sbjct: 1   MAALLESAWRYLITNFSDFQLACFGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           S AAQGKCI RLL+YH GVNLP+MIVSYPVFK MGMRS+LPLPSWKVVFGQI+FYFIVED
Sbjct: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWM LR++ETVEAHCGYHFPWSPSNF+PLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

[Close]

BLAST of Moc03g03390 vs. ExPASy Swiss-Prot
Match: Q8VWZ8 (Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV=1)

HSP 1 Score: 461.8 bits (1187), Expect = 5.0e-129
Identity = 211/259 (81.47%), Postives = 236/259 (91.12%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MA+ +ES W YL+T+FSDFQLA IG+F++HESVFFL GLPFIFLER G+LSKYKIQ K N
Sbjct: 1   MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           +PAAQGKCI RLL+YH  VNLPLM+ SYPVF+ MGMRSS PLPSWK V  QILFYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILH+KWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           +TLWLWM LR++ETVEAHCGYHFPWS SNFLPLYGGA FHDYHHRLLYTKSGNYSSTF Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIKK 260
           MDWIFGTDKG+R L+ +K+
Sbjct: 241 MDWIFGTDKGYRRLKTLKE 259

[Close]

BLAST of Moc03g03390 vs. ExPASy Swiss-Prot
Match: Q9ZW22 (Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana OX=3702 GN=SMO2-1 PE=2 SV=2)

HSP 1 Score: 458.4 bits (1178), Expect = 5.5e-128
Identity = 213/271 (78.60%), Postives = 239/271 (88.19%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           M +++ES W YL+T+FSDFQLA IG+FI+HESVFFL GLP+IFLERTG+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           +P AQGKCI RLL+YHC VNLPLM+ SYPVF+ MGM SS PLPSWKVV  QILFYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGG------------AYFHDYHHRLLY 240
           +TLWLWM LR++ETVEAHCGYHFPWSPSNFLPLYGG            A FHDYHHRLLY
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLY 240

Query: 241 TKSGNYSSTFTYMDWIFGTDKGFRNLEAIKK 260
           TKSGNYSSTF YMDWIFGTDKG+R L+A+K+
Sbjct: 241 TKSGNYSSTFVYMDWIFGTDKGYRKLKALKE 271

[Close]

BLAST of Moc03g03390 vs. ExPASy Swiss-Prot
Match: B8B6I2 (Very-long-chain aldehyde decarbonylase GL1-8 OS=Oryza sativa subsp. indica OX=39946 GN=GL18 PE=3 SV=2)

HSP 1 Score: 422.2 bits (1084), Expect = 4.4e-117
Identity = 192/260 (73.85%), Postives = 226/260 (86.92%), Query Frame = 0


Query: 2   AAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTNS 61
           AA LESAW YLIT+FS+FQLASIGTF++HESVFFL GLP +  ER G  SKYKIQ K+N+
Sbjct: 4   AAGLESAWEYLITHFSEFQLASIGTFLLHESVFFLSGLPSLLFERLGLFSKYKIQKKSNT 63

Query: 62  PAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVEDF 121
           P  Q +C++RL++YH  VNLPL I+SY  FK MG+RS+LPLP W VV  Q+LF+F++EDF
Sbjct: 64  PDYQNRCVVRLVLYHVCVNLPLTILSYRTFKFMGLRSTLPLPHWTVVVSQVLFFFVLEDF 123

Query: 122 VFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHLM 181
           +FYWGHR LHTKWLY++VHSVHHEYATPFGLT+EYAHPAEILFLGFATV GPA+TGPHL 
Sbjct: 124 IFYWGHRALHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATVAGPALTGPHLF 183

Query: 182 TLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTYM 241
           TLW+WM LR++ETVEAH GYHFPWSPSNFLPLYGGA FHDYHHR+LYTKSGNYSSTF YM
Sbjct: 184 TLWVWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGAEFHDYHHRVLYTKSGNYSSTFIYM 243

Query: 242 DWIFGTDKGFRNLEAIKKAE 262
           DW+FGTDK +R  +A+++ E
Sbjct: 244 DWLFGTDKDYRKTKALEEKE 263

[Close]

BLAST of Moc03g03390 vs. ExPASy Swiss-Prot
Match: Q69L93 (Very-long-chain aldehyde decarbonylase GL1-8 OS=Oryza sativa subsp. japonica OX=39947 GN=GL1-8 PE=2 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 4.4e-117
Identity = 192/260 (73.85%), Postives = 226/260 (86.92%), Query Frame = 0


Query: 2   AAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTNS 61
           AA LESAW YLIT+FS+FQLASIGTF++HESVFFL GLP +  ER G  SKYKIQ K+N+
Sbjct: 4   AAGLESAWEYLITHFSEFQLASIGTFLLHESVFFLSGLPSLLFERLGLFSKYKIQKKSNT 63

Query: 62  PAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVEDF 121
           P  Q +C++RL++YH  VNLPL I+SY  FK MG+RS+LPLP W VV  Q+LF+F++EDF
Sbjct: 64  PDYQNRCVVRLVLYHVCVNLPLTILSYRTFKFMGLRSTLPLPHWTVVVSQVLFFFVLEDF 123

Query: 122 VFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHLM 181
           +FYWGHR LHTKWLY++VHSVHHEYATPFGLT+EYAHPAEILFLGFATV GPA+TGPHL 
Sbjct: 124 IFYWGHRALHTKWLYQHVHSVHHEYATPFGLTSEYAHPAEILFLGFATVAGPALTGPHLF 183

Query: 182 TLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTYM 241
           TLW+WM LR++ETVEAH GYHFPWSPSNFLPLYGGA FHDYHHR+LYTKSGNYSSTF YM
Sbjct: 184 TLWVWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGAEFHDYHHRVLYTKSGNYSSTFIYM 243

Query: 242 DWIFGTDKGFRNLEAIKKAE 262
           DW+FGTDK +R  +A+++ E
Sbjct: 244 DWLFGTDKDYRKTKALEEKE 263

[Close]

BLAST of Moc03g03390 vs. ExPASy Swiss-Prot
Match: B8BIM2 (Very-long-chain aldehyde decarbonylase GL1-11 OS=Oryza sativa subsp. indica OX=39946 GN=GL1-11 PE=3 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 7.0e-115
Identity = 182/260 (70.00%), Postives = 227/260 (87.31%), Query Frame = 0


Query: 2   AAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTNS 61
           A+ L+SAW  L  +F++FQLA++ TF++HE+VFFL GLP +  ER G  +KYKIQ K+N+
Sbjct: 3   ASALDSAWEGLTGSFTEFQLATVVTFLLHETVFFLSGLPSLLFERFGLFAKYKIQKKSNT 62

Query: 62  PAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVEDF 121
           P+ Q +C++RL++YH  VNLP+M++SYP FK MG+RSSLPLP W V+  Q+LFYF++EDF
Sbjct: 63  PSYQNRCVLRLILYHVCVNLPVMVLSYPAFKFMGLRSSLPLPHWTVIVSQVLFYFVLEDF 122

Query: 122 VFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHLM 181
           +FYWGHR LHTKWLYK+VHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL 
Sbjct: 123 IFYWGHRALHTKWLYKHVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLF 182

Query: 182 TLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTYM 241
           TLWLWM LR++ETVEAH GYHFPWSPSNFLPLYGG+ FHDYHHR+LYTKSGNY+STF YM
Sbjct: 183 TLWLWMVLRVLETVEAHSGYHFPWSPSNFLPLYGGSDFHDYHHRVLYTKSGNYASTFVYM 242

Query: 242 DWIFGTDKGFRNLEAIKKAE 262
           DW+FGTDK +RN +AI++ +
Sbjct: 243 DWLFGTDKDYRNAKAIEEKD 262

[Close]

BLAST of Moc03g03390 vs. ExPASy TrEMBL
Match: A0A6J1DUY4 (methylsterol monooxygenase 2-2-like OS=Momordica charantia OX=3673 GN=LOC111024686 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 3.0e-153
Identity = 262/262 (100.00%), Postives = 262/262 (100.00%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN
Sbjct: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED
Sbjct: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAEN
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

[Close]

BLAST of Moc03g03390 vs. ExPASy TrEMBL
Match: A0A1S3C6T9 (methylsterol monooxygenase 2-2 OS=Cucumis melo OX=3656 GN=LOC103497129 PE=3 SV=1)

HSP 1 Score: 510.4 bits (1313), Expect = 4.5e-141
Identity = 234/262 (89.31%), Postives = 252/262 (96.18%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAA+LESAW YLITNFSDFQLA IG+FI+HESVFFL GLPFI LER GWLSKYKIQAK N
Sbjct: 1   MAALLESAWQYLITNFSDFQLACIGSFIIHESVFFLSGLPFILLERAGWLSKYKIQAKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCI RLL+YH GVNLP+M+VSYPVFK+MGMRS+LPLPSWKVVFGQI+FYFI+ED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMLVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
            TLWLWM +R++ETVEAHCGYHFPWSPSNF+PLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 FTLWLWMVVRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAEN
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAEN 262

[Close]

BLAST of Moc03g03390 vs. ExPASy TrEMBL
Match: A0A6J1I374 (methylsterol monooxygenase 2-2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111469499 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 235/262 (89.69%), Postives = 252/262 (96.18%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MA +LESAW YLITNFSDFQLA IG+FI+HESVFFL GLPF+FLER GWLSKYKIQAK N
Sbjct: 1   MAELLESAWRYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCI RLL+YH GVNLP+MIVSYPVFK+MGMRS+LPLPSWKVVFGQI+FYFIVED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWM LR++ETVEAHCGY FPWSPSNF+PLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

[Close]

BLAST of Moc03g03390 vs. ExPASy TrEMBL
Match: A0A6J1HLN4 (methylsterol monooxygenase 2-2 OS=Cucurbita moschata OX=3662 GN=LOC111464703 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.0e-140
Identity = 235/262 (89.69%), Postives = 252/262 (96.18%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MA +LESAW YLITNFSDFQLA IG+FI+HESVFFL GLPF+FLER GWLSKYKIQAK N
Sbjct: 1   MAELLESAWRYLITNFSDFQLACIGSFIIHESVFFLSGLPFVFLERAGWLSKYKIQAKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           SPAAQGKCI RLL+YH GVNLP+MIVSYPVFK+MGMRS+LPLPSWKVVFGQI+FYFIVED
Sbjct: 61  SPAAQGKCISRLLLYHFGVNLPVMIVSYPVFKRMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWM LR++ETVEAHCGY FPWSPSNF+PLYGGAYFHDYHHRLLYTKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYDFPWSPSNFIPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

[Close]

BLAST of Moc03g03390 vs. ExPASy TrEMBL
Match: A0A6J1G9R6 (methylsterol monooxygenase 2-2-like OS=Cucurbita moschata OX=3662 GN=LOC111452253 PE=3 SV=1)

HSP 1 Score: 508.1 bits (1307), Expect = 2.3e-140
Identity = 234/262 (89.31%), Postives = 253/262 (96.56%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MAA+LESAW YLITNFSDFQLA +G+FI+HESVFFL GLPFIFLE+ GWLSKYKIQAKTN
Sbjct: 1   MAALLESAWRYLITNFSDFQLACVGSFIIHESVFFLSGLPFIFLEKAGWLSKYKIQAKTN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           S AAQGKCI RLL+YH GVNLP+MIVSYPVFK MGMRS+LPLPSWKVVFGQI+FYFIVED
Sbjct: 61  SSAAQGKCISRLLLYHFGVNLPVMIVSYPVFKLMGMRSTLPLPSWKVVFGQIIFYFIVED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLY+NVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYQNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           MTLWLWM LR++ETVEAHCGYHFPWSPSNF+PLYGGAYFHDYHHRLL+TKSGNYSSTFTY
Sbjct: 181 MTLWLWMVLRVLETVEAHCGYHFPWSPSNFIPLYGGAYFHDYHHRLLFTKSGNYSSTFTY 240

Query: 241 MDWIFGTDKGFRNLEAIKKAEN 263
           MDWIFGTDKGFRNLEAIKKAE+
Sbjct: 241 MDWIFGTDKGFRNLEAIKKAES 262

[Close]

BLAST of Moc03g03390 vs. TAIR 10
Match: AT2G29390.2 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 467.2 bits (1201), Expect = 8.5e-132
Identity = 213/259 (82.24%), Postives = 239/259 (92.28%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           M +++ES W YL+T+FSDFQLA IG+FI+HESVFFL GLP+IFLERTG+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           +P AQGKCI RLL+YHC VNLPLM+ SYPVF+ MGM SS PLPSWKVV  QILFYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           +TLWLWM LR++ETVEAHCGYHFPWSPSNFLPLYGGA FHDYHHRLLYTKSGNYSSTF Y
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIKK 260
           MDWIFGTDKG+R L+A+K+
Sbjct: 241 MDWIFGTDKGYRKLKALKE 259

[Close]

BLAST of Moc03g03390 vs. TAIR 10
Match: AT1G07420.1 (sterol 4-alpha-methyl-oxidase 2-1 )

HSP 1 Score: 461.8 bits (1187), Expect = 3.6e-130
Identity = 211/259 (81.47%), Postives = 236/259 (91.12%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           MA+ +ES W YL+T+FSDFQLA IG+F++HESVFFL GLPFIFLER G+LSKYKIQ K N
Sbjct: 1   MASFVESGWQYLVTHFSDFQLACIGSFLLHESVFFLSGLPFIFLERQGFLSKYKIQTKNN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           +PAAQGKCI RLL+YH  VNLPLM+ SYPVF+ MGMRSS PLPSWK V  QILFYFI+ED
Sbjct: 61  TPAAQGKCITRLLLYHFSVNLPLMLASYPVFRAMGMRSSFPLPSWKEVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILH+KWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHSKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTY 240
           +TLWLWM LR++ETVEAHCGYHFPWS SNFLPLYGGA FHDYHHRLLYTKSGNYSSTF Y
Sbjct: 181 ITLWLWMVLRVLETVEAHCGYHFPWSLSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVY 240

Query: 241 MDWIFGTDKGFRNLEAIKK 260
           MDWIFGTDKG+R L+ +K+
Sbjct: 241 MDWIFGTDKGYRRLKTLKE 259

[Close]

BLAST of Moc03g03390 vs. TAIR 10
Match: AT2G29390.1 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 458.4 bits (1178), Expect = 3.9e-129
Identity = 213/271 (78.60%), Postives = 239/271 (88.19%), Query Frame = 0


Query: 1   MAAILESAWLYLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTN 60
           M +++ES W YL+T+FSDFQLA IG+FI+HESVFFL GLP+IFLERTG+LS YKIQ K+N
Sbjct: 1   MDSLVESGWKYLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSN 60

Query: 61  SPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVED 120
           +P AQGKCI RLL+YHC VNLPLM+ SYPVF+ MGM SS PLPSWKVV  QILFYFI+ED
Sbjct: 61  TPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIED 120

Query: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHL 180
           FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL
Sbjct: 121 FVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHL 180

Query: 181 MTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGG------------AYFHDYHHRLLY 240
           +TLWLWM LR++ETVEAHCGYHFPWSPSNFLPLYGG            A FHDYHHRLLY
Sbjct: 181 ITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGSLILMWESFAYSADFHDYHHRLLY 240

Query: 241 TKSGNYSSTFTYMDWIFGTDKGFRNLEAIKK 260
           TKSGNYSSTF YMDWIFGTDKG+R L+A+K+
Sbjct: 241 TKSGNYSSTFVYMDWIFGTDKGYRKLKALKE 271

[Close]

BLAST of Moc03g03390 vs. TAIR 10
Match: AT2G29390.3 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 458.4 bits (1178), Expect = 3.9e-129
Identity = 209/249 (83.94%), Postives = 232/249 (93.17%), Query Frame = 0


Query: 11  YLITNFSDFQLASIGTFIVHESVFFLFGLPFIFLERTGWLSKYKIQAKTNSPAAQGKCII 70
           YL+T+FSDFQLA IG+FI+HESVFFL GLP+IFLERTG+LS YKIQ K+N+P AQGKCI 
Sbjct: 4   YLVTHFSDFQLACIGSFILHESVFFLSGLPYIFLERTGFLSNYKIQTKSNTPEAQGKCIA 63

Query: 71  RLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFIVEDFVFYWGHRIL 130
           RLL+YHC VNLPLM+ SYPVF+ MGM SS PLPSWKVV  QILFYFI+EDFVFYWGHRIL
Sbjct: 64  RLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFIIEDFVFYWGHRIL 123

Query: 131 HTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITGPHLMTLWLWMTLR 190
           HTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TGPHL+TLWLWM LR
Sbjct: 124 HTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTGPHLITLWLWMMLR 183

Query: 191 LMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSSTFTYMDWIFGTDKG 250
           ++ETVEAHCGYHFPWSPSNFLPLYGGA FHDYHHRLLYTKSGNYSSTF YMDWIFGTDKG
Sbjct: 184 VIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSSTFVYMDWIFGTDKG 243

Query: 251 FRNLEAIKK 260
           +R L+A+K+
Sbjct: 244 YRKLKALKE 252

[Close]

BLAST of Moc03g03390 vs. TAIR 10
Match: AT2G29390.5 (sterol 4-alpha-methyl-oxidase 2-2 )

HSP 1 Score: 384.8 bits (987), Expect = 5.5e-107
Identity = 172/202 (85.15%), Postives = 189/202 (93.56%), Query Frame = 0


Query: 58  KTNSPAAQGKCIIRLLMYHCGVNLPLMIVSYPVFKQMGMRSSLPLPSWKVVFGQILFYFI 117
           K+N+P AQGKCI RLL+YHC VNLPLM+ SYPVF+ MGM SS PLPSWKVV  QILFYFI
Sbjct: 17  KSNTPEAQGKCIARLLLYHCCVNLPLMMASYPVFRFMGMESSFPLPSWKVVSAQILFYFI 76

Query: 118 VEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTAEYAHPAEILFLGFATVIGPAITG 177
           +EDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLT+EYAHPAEILFLGFAT++GPA+TG
Sbjct: 77  IEDFVFYWGHRILHTKWLYKNVHSVHHEYATPFGLTSEYAHPAEILFLGFATIVGPALTG 136

Query: 178 PHLMTLWLWMTLRLMETVEAHCGYHFPWSPSNFLPLYGGAYFHDYHHRLLYTKSGNYSST 237
           PHL+TLWLWM LR++ETVEAHCGYHFPWSPSNFLPLYGGA FHDYHHRLLYTKSGNYSST
Sbjct: 137 PHLITLWLWMMLRVIETVEAHCGYHFPWSPSNFLPLYGGADFHDYHHRLLYTKSGNYSST 196

Query: 238 FTYMDWIFGTDKGFRNLEAIKK 260
           F YMDWIFGTDKG+R L+A+K+
Sbjct: 197 FVYMDWIFGTDKGYRKLKALKE 218

The following BLAST results are available for this feature:

BLAST of Moc03g03390 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158130.1	6.3e-153	100.00	methylsterol monooxygenase 2-2-like [Momordica charantia]	[more]
XP_008457442.1	9.4e-141	89.31	PREDICTED: methylsterol monooxygenase 2-2 [Cucumis melo]	[more]
XP_022964698.1	2.1e-140	89.69	methylsterol monooxygenase 2-2 [Cucurbita moschata] >XP_022970560.1 methylsterol...	[more]
XP_022948631.1	4.6e-140	89.31	methylsterol monooxygenase 2-2-like [Cucurbita moschata] >KAG6607270.1 Methylste...	[more]
XP_022998327.1	1.0e-139	89.31	methylsterol monooxygenase 2-2-like [Cucurbita maxima]	[more]

BLAST of Moc03g03390 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VWZ8	5.0e-129	81.47	Methylsterol monooxygenase 2-2 OS=Arabidopsis thaliana OX=3702 GN=SMO2-2 PE=2 SV...	[more]
Q9ZW22	5.5e-128	78.60	Methylsterol monooxygenase 2-1 OS=Arabidopsis thaliana OX=3702 GN=SMO2-1 PE=2 SV...	[more]
B8B6I2	4.4e-117	73.85	Very-long-chain aldehyde decarbonylase GL1-8 OS=Oryza sativa subsp. indica OX=39...	[more]
Q69L93	4.4e-117	73.85	Very-long-chain aldehyde decarbonylase GL1-8 OS=Oryza sativa subsp. japonica OX=...	[more]
B8BIM2	7.0e-115	70.00	Very-long-chain aldehyde decarbonylase GL1-11 OS=Oryza sativa subsp. indica OX=3...	[more]

BLAST of Moc03g03390 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DUY4	3.0e-153	100.00	methylsterol monooxygenase 2-2-like OS=Momordica charantia OX=3673 GN=LOC1110246...	[more]
A0A1S3C6T9	4.5e-141	89.31	methylsterol monooxygenase 2-2 OS=Cucumis melo OX=3656 GN=LOC103497129 PE=3 SV=1	[more]
A0A6J1I374	1.0e-140	89.69	methylsterol monooxygenase 2-2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1HLN4	1.0e-140	89.69	methylsterol monooxygenase 2-2 OS=Cucurbita moschata OX=3662 GN=LOC111464703 PE=...	[more]
A0A6J1G9R6	2.3e-140	89.31	methylsterol monooxygenase 2-2-like OS=Cucurbita moschata OX=3662 GN=LOC11145225...	[more]

BLAST of Moc03g03390 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G29390.2	8.5e-132	82.24	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT1G07420.1	3.6e-130	81.47	sterol 4-alpha-methyl-oxidase 2-1	[more]
AT2G29390.1	3.9e-129	78.60	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.3	3.9e-129	83.94	sterol 4-alpha-methyl-oxidase 2-2	[more]
AT2G29390.5	5.5e-107	85.15	sterol 4-alpha-methyl-oxidase 2-2	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006694	Fatty acid hydroxylase	PFAM	PF04116	FA_hydroxylase	coord: 113..247 e-value: 1.6E-29 score: 102.9
None	No IPR available	PANTHER	PTHR11863	STEROL DESATURASE	coord: 1..260
None	No IPR available	PANTHER	PTHR11863:SF192	BNAC05G05170D PROTEIN	coord: 1..260

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc03g03390.1	Moc03g03390.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016491	oxidoreductase activity