Moc03g02430 (gene) Bitter gourd (OHB3-1) v2

BLAST of Moc03g02430 vs. NCBI nr
Match: XP_022150946.1 (cytosolic enolase 3 [Momordica charantia])

HSP 1 Score: 960.3 bits (2481), Expect = 6.3e-276
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA
Sbjct: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TTY 484
           TTY
Sbjct: 481 TTY 483

BLAST of Moc03g02430 vs. NCBI nr
Match: XP_022949451.1 (cytosolic enolase 3 [Cucurbita moschata] >XP_023525808.1 cytosolic enolase 3 [Cucurbita pepo subsp. pepo] >KAG7036966.1 Cytosolic enolase 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 908.3 bits (2346), Expect = 2.8e-260
Identity = 453/483 (93.79%), Postives = 466/483 (96.48%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKA+PS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVI+EKHGA GCNFG+DGG APNISSF E LDLVQEAIGRTGYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTK+FS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE+TCNALLLKVNQIGTVTEAI+VV LAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TTY 484
           T Y
Sbjct: 481 TMY 483

BLAST of Moc03g02430 vs. NCBI nr
Match: XP_004145264.1 (cytosolic enolase 3 [Cucumis sativus] >KGN65781.1 hypothetical protein Csa_023204 [Cucumis sativus])

HSP 1 Score: 906.4 bits (2341), Expect = 1.1e-259
Identity = 454/480 (94.58%), Postives = 466/480 (97.08%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKAIPS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVITEKHGA GCNFG+DGG APNISSFKE LDLV+EAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK+FSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE TCNALLLKVNQIGTVTEAI+VVKLAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Moc03g02430 vs. NCBI nr
Match: XP_008457408.1 (PREDICTED: cytosolic enolase 3 [Cucumis melo])

HSP 1 Score: 903.7 bits (2334), Expect = 7.0e-259
Identity = 453/480 (94.38%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKAIPS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVITEKHGA  CNFG+DGG APNISSFKE LDLV+EAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK+FSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE TCNALLLKVNQIGTVTEAI+VVKLAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Moc03g02430 vs. NCBI nr
Match: XP_038893436.1 (cytosolic enolase 3 [Benincasa hispida])

HSP 1 Score: 900.6 bits (2326), Expect = 5.9e-258
Identity = 449/480 (93.54%), Postives = 464/480 (96.67%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDK+MLSRKIEEAVNAAVRAKT DPVLFISNHMKKAIPS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKFMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGA KFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGACKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLK VITEKHGA GCNFG+DGG APNISSFKE LDLV+EAIGR GYNERIKIAIDVAATN
Sbjct: 241 HLKVVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAIGRAGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK+FSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK R+ERAIDE TCNALLLKVNQIGTVTEAI+VVK+AK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRMERAIDEFTCNALLLKVNQIGTVTEAIEVVKVAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQ +YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQGIYAGEDWR 480

BLAST of Moc03g02430 vs. ExPASy Swiss-Prot
Match: Q9ZW34 (Cytosolic enolase 3 OS=Arabidopsis thaliana OX=3702 GN=ENO3 PE=1 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 2.8e-226
Identity = 383/472 (81.14%), Postives = 433/472 (91.74%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKA+ S+ITKVKARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKNINEKISEA 128
           IPTVEVDL+TNKGVF ASVPSGD +G YEA++LRDGDKGMYLGNSV KAVKNINEKISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPLYKHIADLS 188
           LIG DP  Q +IDQAM DLD TEKK+ELGANAILAVSIA C+AGAAEK+VPL KH++DLS
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITE 248
           G+ N++LPVPAFTV+SGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVI+E
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 249 KHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATNFCMGTKYD 308
           K+G  GCN G+DGGLAP+ISS KEGL+LV+EAI RTGYN++IKIAID+AATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 309 LDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSSLGLCQVVG 368
           LD K+PNKS QNFKS +DMIDMYKE+C DYPIVSIEDPFD+EDW+HTK FSSLG+CQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 369 GDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVASHRCGETD 428
            DLLMSN  R+ERAI ES+CNALLLKVNQIGTVTEAI+VVK+A+ AQWGVV SHRCGET+
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 429 DTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           D+F++D SVGL+TG IKAGAPCRGER  KYNQLLRIEEELGDQAVYAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of Moc03g02430 vs. ExPASy Swiss-Prot
Match: Q27527 (Enolase OS=Caenorhabditis elegans OX=6239 GN=enol-1 PE=1 SV=3)

HSP 1 Score: 507.3 bits (1305), Expect = 1.9e-142
Identity = 252/428 (58.88%), Postives = 315/428 (73.60%), Query Frame = 0

Query: 55  ITKVKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSV 114
           ITK+ ARQI DSRG PTVEVDL+T KGVF A+VPSG   G++EA++LRDGDK ++LG  V
Sbjct: 3   ITKIHARQIYDSRGNPTVEVDLFTEKGVFRAAVPSGASTGVHEALELRDGDKAVHLGKGV 62

Query: 115 TKAVKNINEKISEALI--GRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAG 174
            KAV NINEKI+ ALI  G D T Q +ID  M  LD +E K  LGANAIL VS+AV +AG
Sbjct: 63  LKAVSNINEKIAPALIAKGFDVTAQKDIDDFMMALDGSENKGNLGANAILGVSLAVAKAG 122

Query: 175 AAEKDVPLYKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A  K +PLYK+IA+L+G   ++LPVPAF VI+GG HAGN LA+QE MILP+GAS F EA+
Sbjct: 123 AVHKGLPLYKYIAELAGTGKVVLPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFAEAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKI 294
           +MGSE +HHLKA I +++G      GD+GG APNI   KEGLDL+  AI + GY  +I I
Sbjct: 183 RMGSEVYHHLKAEIKKRYGLDATAVGDEGGFAPNIQDNKEGLDLLNTAIDKAGYTGKISI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK P      + SG+ + ++Y+    +YP+VSIED FD++DW
Sbjct: 243 GMDVAASEFFKDGKYDLDFKNPASDSSKWLSGEQLTELYQSFIKEYPVVSIEDAFDQDDW 302

Query: 355 DHTKNFSSLGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAK 414
           D+   F      Q+VG DL ++N  RI+ AID+ +CN LLLKVNQIG+VTE+I+  KL++
Sbjct: 303 DNWGKFHGATSIQLVGDDLTVTNPKRIQTAIDKKSCNCLLLKVNQIGSVTESIEAAKLSR 362

Query: 415 VAQWGVVASHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              WGV+ SHR GET+DTF+AD  VGL+TGQIK GAPCR ERLAKYNQLLRIEEELG  A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLATGQIKTGAPCRSERLAKYNQLLRIEEELGADA 422

Query: 475 VYAGEDWR 481
           VYAG ++R
Sbjct: 423 VYAGHNFR 430

BLAST of Moc03g02430 vs. ExPASy Swiss-Prot
Match: Q4R5L2 (Alpha-enolase OS=Macaca fascicularis OX=9541 GN=ENO1 PE=2 SV=1)

HSP 1 Score: 506.1 bits (1302), Expect = 4.3e-142
Identity = 246/428 (57.48%), Postives = 325/428 (75.93%), Query Frame = 0

Query: 55  ITKVKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSV 114
           I KV AR+I DSRG PTVEVDL+T+KG+F A+VPSG   G+YEA++LRD DK  Y+G  V
Sbjct: 3   ILKVHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGV 62

Query: 115 TKAVKNINEKISEALIGR--DPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAG 174
           +KAV++IN+ I+ AL+ +  + T+Q +ID+ M ++D TE K++ GANAIL VS+AVC+AG
Sbjct: 63  SKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKDVPLYKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A EK VPLY+HIADL+G + +ILPVPAF VI+GG HAGN LA+QE MILP+GA+ F EA+
Sbjct: 123 AVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKI 294
           ++G+E +H+LK VI EK+G    N GD+GG APNI   KEGL+L++ AIG+ GY +++ I
Sbjct: 183 RIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK+P+     + S   + D+YK    DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFFRSGKYDLDFKSPD-DPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDW 302

Query: 355 DHTKNFSSLGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAK 414
              + F++    QVVG DL ++N  RI +A++E +CN LLLKVNQIG+VTE++   KLA+
Sbjct: 303 GAWQKFTASAGIQVVGDDLTVANPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQ 362

Query: 415 VAQWGVVASHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              WGV+ SHR GET+DTF+AD  VGL TGQIK GAPCR ERLAKYNQLLRIEEELG +A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKA 422

Query: 475 VYAGEDWR 481
            +AG ++R
Sbjct: 423 KFAGRNFR 429

BLAST of Moc03g02430 vs. ExPASy Swiss-Prot
Match: P06733 (Alpha-enolase OS=Homo sapiens OX=9606 GN=ENO1 PE=1 SV=2)

HSP 1 Score: 505.8 bits (1301), Expect = 5.6e-142
Identity = 245/428 (57.24%), Postives = 325/428 (75.93%), Query Frame = 0

Query: 55  ITKVKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSV 114
           I K+ AR+I DSRG PTVEVDL+T+KG+F A+VPSG   G+YEA++LRD DK  Y+G  V
Sbjct: 3   ILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGV 62

Query: 115 TKAVKNINEKISEALIGR--DPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAG 174
           +KAV++IN+ I+ AL+ +  + T+Q +ID+ M ++D TE K++ GANAIL VS+AVC+AG
Sbjct: 63  SKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKDVPLYKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A EK VPLY+HIADL+G + +ILPVPAF VI+GG HAGN LA+QE MILP+GA+ F EA+
Sbjct: 123 AVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKI 294
           ++G+E +H+LK VI EK+G    N GD+GG APNI   KEGL+L++ AIG+ GY +++ I
Sbjct: 183 RIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK+P+     + S   + D+YK    DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFFRSGKYDLDFKSPD-DPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDW 302

Query: 355 DHTKNFSSLGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAK 414
              + F++    QVVG DL ++N  RI +A++E +CN LLLKVNQIG+VTE++   KLA+
Sbjct: 303 GAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNQIGSVTESLQACKLAQ 362

Query: 415 VAQWGVVASHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              WGV+ SHR GET+DTF+AD  VGL TGQIK GAPCR ERLAKYNQLLRIEEELG +A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKA 422

Query: 475 VYAGEDWR 481
            +AG ++R
Sbjct: 423 KFAGRNFR 429

BLAST of Moc03g02430 vs. ExPASy Swiss-Prot
Match: Q5R6Y1 (Alpha-enolase OS=Pongo abelii OX=9601 GN=ENO1 PE=2 SV=1)

HSP 1 Score: 504.2 bits (1297), Expect = 1.6e-141
Identity = 244/428 (57.01%), Postives = 325/428 (75.93%), Query Frame = 0

Query: 55  ITKVKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSV 114
           I K+ AR+I DSRG PTVEVDL+T+KG+F A+VPSG   G+YEA++LRD DK  Y+G  V
Sbjct: 3   ILKIHAREIFDSRGNPTVEVDLFTSKGLFRAAVPSGASTGIYEALELRDNDKTRYMGKGV 62

Query: 115 TKAVKNINEKISEALIGR--DPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAG 174
           +KAV++IN+ I+ AL+ +  + T+Q +ID+ M ++D TE K++ GANAIL VS+AVC+AG
Sbjct: 63  SKAVEHINKTIAPALVSKKLNVTEQEKIDKLMIEMDGTENKSKFGANAILGVSLAVCKAG 122

Query: 175 AAEKDVPLYKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEAL 234
           A EK VPLY+HIADL+G + +ILPVPAF VI+GG HAGN LA+QE MILP+GA+ F EA+
Sbjct: 123 AVEKGVPLYRHIADLAGNSEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGAANFREAM 182

Query: 235 KMGSETFHHLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKI 294
           ++G+E +H+LK VI EK+G    N GD+GG APNI   KEGL+L++ AIG+ GY +++ I
Sbjct: 183 RIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEGLELLKTAIGKAGYTDKVVI 242

Query: 295 AIDVAATNFCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDW 354
            +DVAA+ F    KYDLDFK+P+     + S   + D+YK    DYP+VSIEDPFD++DW
Sbjct: 243 GMDVAASEFFRSGKYDLDFKSPD-DPSRYISPDQLADLYKSFIKDYPVVSIEDPFDQDDW 302

Query: 355 DHTKNFSSLGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAK 414
              + F++    QVVG DL ++N  RI +A++E +CN LLLKVN+IG+VTE++   KLA+
Sbjct: 303 GAWQKFTASAGIQVVGDDLTVTNPKRIAKAVNEKSCNCLLLKVNRIGSVTESLQACKLAQ 362

Query: 415 VAQWGVVASHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQA 474
              WGV+ SHR GET+DTF+AD  VGL TGQIK GAPCR ERLAKYNQLLRIEEELG +A
Sbjct: 363 ANGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQLLRIEEELGSKA 422

Query: 475 VYAGEDWR 481
            +AG ++R
Sbjct: 423 KFAGRNFR 429

BLAST of Moc03g02430 vs. ExPASy TrEMBL
Match: A0A6J1DD28 (Phosphopyruvate hydratase OS=Momordica charantia OX=3673 GN=LOC111018976 PE=3 SV=1)

HSP 1 Score: 960.3 bits (2481), Expect = 3.1e-276
Identity = 483/483 (100.00%), Postives = 483/483 (100.00%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA
Sbjct: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TTY 484
           TTY
Sbjct: 481 TTY 483

BLAST of Moc03g02430 vs. ExPASy TrEMBL
Match: A0A6J1GC24 (Phosphopyruvate hydratase OS=Cucurbita moschata OX=3662 GN=LOC111452790 PE=3 SV=1)

HSP 1 Score: 908.3 bits (2346), Expect = 1.4e-260
Identity = 453/483 (93.79%), Postives = 466/483 (96.48%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKA+PS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           IN+KISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVI+EKHGA GCNFG+DGG APNISSF E LDLVQEAIGRTGYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTK+FS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE+TCNALLLKVNQIGTVTEAI+VV LAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TTY 484
           T Y
Sbjct: 481 TMY 483

BLAST of Moc03g02430 vs. ExPASy TrEMBL
Match: A0A0A0M0P5 (Phosphopyruvate hydratase OS=Cucumis sativus OX=3659 GN=Csa_1G528460 PE=3 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.2e-260
Identity = 454/480 (94.58%), Postives = 466/480 (97.08%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKAIPS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVITEKHGA GCNFG+DGG APNISSFKE LDLV+EAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHGCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK+FSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE TCNALLLKVNQIGTVTEAI+VVKLAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Moc03g02430 vs. ExPASy TrEMBL
Match: A0A1S3C645 (Phosphopyruvate hydratase OS=Cucumis melo OX=3656 GN=LOC103497104 PE=3 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.4e-259
Identity = 453/480 (94.38%), Postives = 465/480 (96.88%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKAIPS+ITK+KA
Sbjct: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAIPSVITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAV+LRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVELRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           INEKISEALIGRDPTQQYEIDQAMKDLDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTV+SGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVLSGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVITEKHGA  CNFG+DGG APNISSFKE LDLV+EAI R GYNERIKIAIDVAATN
Sbjct: 241 HLKAVITEKHGAHDCNFGEDGGFAPNISSFKEALDLVEEAINRGGYNERIKIAIDVAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK+FSS
Sbjct: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKHFSS 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE TCNALLLKVNQIGTVTEAI+VVKLAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDEFTCNALLLKVNQIGTVTEAIEVVKLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLST QIKAGAPCRGERLAKYNQLLRIEEELGDQA+YAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTCQIKAGAPCRGERLAKYNQLLRIEEELGDQAIYAGEDWR 480

BLAST of Moc03g02430 vs. ExPASy TrEMBL
Match: A0A6J1KFE0 (Phosphopyruvate hydratase OS=Cucurbita maxima OX=3661 GN=LOC111493370 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.1e-257
Identity = 448/483 (92.75%), Postives = 465/483 (96.27%), Query Frame = 0

Query: 1   MEKKQLKEMSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKA 60
           MEKKQLK+MSVQEYLDKYMLSRKIEEAVNAAVRAKT DPVLFISNHMKKA+PS+ITK+KA
Sbjct: 1   MEKKQLKQMSVQEYLDKYMLSRKIEEAVNAAVRAKTPDPVLFISNHMKKAVPSLITKIKA 60

Query: 61  RQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKN 120
           RQILDSRGIPTVEVDL+TNKGVF+ASVPSGDPAGMYEAVDLRDGDKG YLGNSVTKAVKN
Sbjct: 61  RQILDSRGIPTVEVDLHTNKGVFNASVPSGDPAGMYEAVDLRDGDKGTYLGNSVTKAVKN 120

Query: 121 INEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPL 180
           IN+KISEALIGRDPTQQYEIDQAMK+LDTTEKK ELGANAILAVSIAVCRAGAAEK+VPL
Sbjct: 121 INDKISEALIGRDPTQQYEIDQAMKNLDTTEKKGELGANAILAVSIAVCRAGAAEKEVPL 180

Query: 181 YKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240
           YKHIADL+GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH
Sbjct: 181 YKHIADLAGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFH 240

Query: 241 HLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATN 300
           HLKAVI+EKHGA GCNFG+DGG APNISSF E LDLVQEAIGRTGYNERIKIAID+AATN
Sbjct: 241 HLKAVISEKHGAHGCNFGEDGGFAPNISSFTEALDLVQEAIGRTGYNERIKIAIDIAATN 300

Query: 301 FCMGTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSS 360
           FCMGTKYDLDFKAPNK  QNFKSGKDMIDMYKELC DYPIVSIEDPFDREDWDHTK+FS 
Sbjct: 301 FCMGTKYDLDFKAPNKLGQNFKSGKDMIDMYKELCDDYPIVSIEDPFDREDWDHTKHFSG 360

Query: 361 LGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVA 420
           LG+CQVVGGDLLMSNK RIERAIDE+TCNALLLKVNQIGTVTEAI+VV LAK AQW VVA
Sbjct: 361 LGICQVVGGDLLMSNKKRIERAIDETTCNALLLKVNQIGTVTEAIEVVNLAKDAQWAVVA 420

Query: 421 SHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480
           SHRCGETDDTFLAD SVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR
Sbjct: 421 SHRCGETDDTFLADLSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 480

Query: 481 TTY 484
             Y
Sbjct: 481 AMY 483

BLAST of Moc03g02430 vs. TAIR 10
Match: AT2G29560.1 (cytosolic enolase )

HSP 1 Score: 785.8 bits (2028), Expect = 2.0e-227
Identity = 383/472 (81.14%), Postives = 433/472 (91.74%), Query Frame = 0

Query: 9   MSVQEYLDKYMLSRKIEEAVNAAVRAKTLDPVLFISNHMKKAIPSMITKVKARQILDSRG 68
           MSVQEYLDK+MLSRKIE+AVNAAVRAKT DPVLFI+NH+KKA+ S+ITKVKARQILDSRG
Sbjct: 1   MSVQEYLDKHMLSRKIEDAVNAAVRAKTSDPVLFIANHLKKAVSSVITKVKARQILDSRG 60

Query: 69  IPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKAVKNINEKISEA 128
           IPTVEVDL+TNKGVF ASVPSGD +G YEA++LRDGDKGMYLGNSV KAVKNINEKISEA
Sbjct: 61  IPTVEVDLHTNKGVFRASVPSGDSSGTYEAIELRDGDKGMYLGNSVAKAVKNINEKISEA 120

Query: 129 LIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKDVPLYKHIADLS 188
           LIG DP  Q +IDQAM DLD TEKK+ELGANAILAVSIA C+AGAAEK+VPL KH++DLS
Sbjct: 121 LIGMDPKLQGQIDQAMIDLDKTEKKSELGANAILAVSIAACKAGAAEKEVPLCKHLSDLS 180

Query: 189 GKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSETFHHLKAVITE 248
           G+ N++LPVPAFTV+SGGKHA N  AIQEIMILPIGAS+FEEAL+ GSET+HHLKAVI+E
Sbjct: 181 GRANMVLPVPAFTVLSGGKHASNTFAIQEIMILPIGASRFEEALQWGSETYHHLKAVISE 240

Query: 249 KHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVAATNFCMGTKYD 308
           K+G  GCN G+DGGLAP+ISS KEGL+LV+EAI RTGYN++IKIAID+AATNFC+GTKYD
Sbjct: 241 KNGGLGCNVGEDGGLAPDISSLKEGLELVKEAINRTGYNDKIKIAIDIAATNFCLGTKYD 300

Query: 309 LDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTKNFSSLGLCQVVG 368
           LD K+PNKS QNFKS +DMIDMYKE+C DYPIVSIEDPFD+EDW+HTK FSSLG+CQVVG
Sbjct: 301 LDIKSPNKSGQNFKSAEDMIDMYKEICNDYPIVSIEDPFDKEDWEHTKYFSSLGICQVVG 360

Query: 369 GDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQWGVVASHRCGETD 428
            DLLMSN  R+ERAI ES+CNALLLKVNQIGTVTEAI+VVK+A+ AQWGVV SHRCGET+
Sbjct: 361 DDLLMSNSKRVERAIQESSCNALLLKVNQIGTVTEAIEVVKMARDAQWGVVTSHRCGETE 420

Query: 429 DTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAGEDWR 481
           D+F++D SVGL+TG IKAGAPCRGER  KYNQLLRIEEELGDQAVYAGEDW+
Sbjct: 421 DSFISDLSVGLATGVIKAGAPCRGERTMKYNQLLRIEEELGDQAVYAGEDWK 472

BLAST of Moc03g02430 vs. TAIR 10
Match: AT2G36530.1 (Enolase )

HSP 1 Score: 496.5 bits (1277), Expect = 2.4e-140
Identity = 257/436 (58.94%), Postives = 325/436 (74.54%), Query Frame = 0

Query: 55  ITKVKARQILDSRGIPTVEVDLYTNKGV-FHASVPSGDPAGMYEAVDLRDGDKGMYLGNS 114
           IT VKARQI DSRG PTVEVD++T+ G+   A+VPSG   G+YEA++LRDG    YLG  
Sbjct: 4   ITVVKARQIFDSRGNPTVEVDIHTSNGIKVTAAVPSGASTGIYEALELRDGGSD-YLGKG 63

Query: 115 VTKAVKNINEKISEALIGRDPTQQYEIDQAM-KDLDTTEK-----KAELGANAILAVSIA 174
           V+KAV N+N  I  ALIG+DPTQQ  ID  M  +LD T+      K +LGANAILAVS+A
Sbjct: 64  VSKAVGNVNNIIGPALIGKDPTQQTAIDNFMVHELDGTQNEWGWCKQKLGANAILAVSLA 123

Query: 175 VCRAGAAEKDVPLYKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASK 234
           VC+AGA    +PLYKHIA+L+G   ++LPVPAF VI+GG HAGN LA+QE MILP+GA+ 
Sbjct: 124 VCKAGAVVSGIPLYKHIANLAGNPKIVLPVPAFNVINGGSHAGNKLAMQEFMILPVGAAS 183

Query: 235 FEEALKMGSETFHHLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYN 294
           F+EA+KMG E +HHLK+VI +K+G    N GD+GG APNI   KEGL+L++ AI + GY 
Sbjct: 184 FKEAMKMGVEVYHHLKSVIKKKYGQDATNVGDEGGFAPNIQENKEGLELLKTAIEKAGYT 243

Query: 295 ERIKIAIDVAATNFCMGTK-YDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDP 354
            ++ I +DVAA+ F    K YDL+FK  N +     SG  + D+YK   A+YPIVSIEDP
Sbjct: 244 GKVVIGMDVAASEFYSEDKTYDLNFKEENNNGSQKISGDALKDLYKSFVAEYPIVSIEDP 303

Query: 355 FDREDWDH-TKNFSSLGL-CQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEA 414
           FD++DW+H  K  +  G   Q+VG DLL++N  R+ +AI E +CNALLLKVNQIG+VTE+
Sbjct: 304 FDQDDWEHYAKMTTECGTEVQIVGDDLLVTNPKRVAKAIAEKSCNALLLKVNQIGSVTES 363

Query: 415 IDVVKLAKVAQWGVVASHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRI 474
           I+ VK++K A WGV+ SHR GET+DTF+AD +VGLSTGQIK GAPCR ERLAKYNQLLRI
Sbjct: 364 IEAVKMSKKAGWGVMTSHRSGETEDTFIADLAVGLSTGQIKTGAPCRSERLAKYNQLLRI 423

Query: 475 EEELGDQAVYAGEDWR 481
           EEELG +A+YAG ++R
Sbjct: 424 EEELGSEAIYAGVNFR 438

BLAST of Moc03g02430 vs. TAIR 10
Match: AT1G74030.1 (enolase 1 )

HSP 1 Score: 476.5 bits (1225), Expect = 2.6e-134
Identity = 240/425 (56.47%), Postives = 309/425 (72.71%), Query Frame = 0

Query: 58  VKARQILDSRGIPTVEVDLYTNKGVFHASVPSGDPAGMYEAVDLRDGDKGMYLGNSVTKA 117
           VKARQI+DSRG PTVEVDL T+  ++ ++VPSG   G+YEA++LRDGDK +Y G  V +A
Sbjct: 54  VKARQIIDSRGNPTVEVDLITD-DLYRSAVPSGASTGIYEALELRDGDKSVYGGKGVLQA 113

Query: 118 VKNINEKISEALIGRDPTQQYEIDQAMKDLDTTEKKAELGANAILAVSIAVCRAGAAEKD 177
           +KNINE ++  LIG D   Q ++D  M +LD T  K++LGANAIL VS++VCRAGA  K 
Sbjct: 114 IKNINELVAPKLIGVDVRNQADVDALMLELDGTPNKSKLGANAILGVSLSVCRAGAGAKG 173

Query: 178 VPLYKHIADLSGKTNLILPVPAFTVISGGKHAGNNLAIQEIMILPIGASKFEEALKMGSE 237
           VPLYKHI + SG   L++PVPAF VI+GG HAGN+LA+QE MILP+GA+ F EA +MGSE
Sbjct: 174 VPLYKHIQETSGTKELVMPVPAFNVINGGSHAGNSLAMQEFMILPVGATSFSEAFQMGSE 233

Query: 238 TFHHLKAVITEKHGAQGCNFGDDGGLAPNISSFKEGLDLVQEAIGRTGYNERIKIAIDVA 297
            +H LK +I  K+G   CN GD+GG APN+   +EGL L+ +AI + GY  +IKI +DVA
Sbjct: 234 VYHTLKGIIKTKYGQDACNVGDEGGFAPNVQDNREGLVLLIDAIEKAGYTGKIKIGMDVA 293

Query: 298 ATNFCM-GTKYDLDFKAPNKSVQNFKSGKDMIDMYKELCADYPIVSIEDPFDREDWDHTK 357
           A+ F M   +YDL+FK       +  S + + D+Y+E   D+PIVSIEDPFD++DW    
Sbjct: 294 ASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADLYREFIKDFPIVSIEDPFDQDDWSSWA 353

Query: 358 NFSSLGLCQVVGGDLLMSNKMRIERAIDESTCNALLLKVNQIGTVTEAIDVVKLAKVAQW 417
           +  S    Q+VG DLL++N  RI  AI + +CNALLLKVNQIGTVTE+I     +K A W
Sbjct: 354 SLQSSVDIQLVGDDLLVTNPKRIAEAIKKQSCNALLLKVNQIGTVTESIQAALDSKAAGW 413

Query: 418 GVVASHRCGETDDTFLADFSVGLSTGQIKAGAPCRGERLAKYNQLLRIEEELGDQAVYAG 477
           GV+ SHR GET+D F+AD SVGL++GQIK GAPCR ERL+KYNQLLRIEEELG+   YAG
Sbjct: 414 GVMVSHRSGETEDNFIADLSVGLASGQIKTGAPCRSERLSKYNQLLRIEEELGN-VRYAG 473

Query: 478 EDWRT 482
           E +R+
Sbjct: 474 EAFRS 476