Moc03g01710 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc03g01710
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPhosphoinositide phospholipase C
Locationchr3: 1296603 .. 1299090 (-)
RNA-Seq ExpressionMoc03g01710
SyntenyMoc03g01710
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGAAATTACAGGGTCTGTCTCTGCTTCATCAGAAGGTTCAAGATGTCCAAGGACGAGCCCCCCTCCGACGTCAAGGAAGCCTTCCTCAACTACGCCGGCGCCGCCGCCACCATGACCGCCGACCAGCTCCGCTCCTTTCTCCTCGACTTTCAGGGCGATCCCGCGCCCTCGCTCGCCGACGCTCACCGGATCATCGACCAAGTCCTGCACCGGCGCCACCACGTCGTGTCCAAGCTCACCAAGCACACTCTCACTCTTGACGATTTCTACTACTTCCTCTTCTCTGTGGATCTCAATCCCCCAATTTCCGATCAGGTAACTTCAAATTCCCAACAATTCCTGAAATGAAATCTCAGCTTGAATTCTGCCTCAAGTTCTTAGATGTATGTGCTAGTGTTCATCACTTTCAGGTCCATCAGGATATGAGAGCTCCATTGTCCCACTATTACGTTTACACTAGCCACAATACGTACCTGACTGGGAACCAATTGAGCAGTGATTCCAGCGATGTGCCGATTATCAATGCGCTGAAGAGCGGCGTGCGAGTCGTCGAGCTTGATTTGTGGCCCAACTCTGAGGAAGATGACATAGATGTTCTTCATGGAAGGTGAGAGAGTTCCACAATCCAACTGTTTTGTGTATTGAATGAACTTTCTGAAGTTCCTTTTTCCATGTAGGACCTTGACAACACCTGTGGAATTTGTTAGATGTCTGAAATCAATTAAAGAGAATGCTTTCTTAGCATCTCCATATCCTGTAATAATTACTCTAGAAGACCACTTAACTCCTGATCTTCAAGCTAAAGCAGCTGAGGTATCTTCTTTTTTACCCCTCCATTTCGTTACAGTATACGAAGAGAAGTAGTTTTGTTCAAACTATGCTTGTGTTTGTTTGTAGAACATAAAGGAAACATTTGGGGACATGCTGTTCGTTCCTGAATCGGAATATCAACAAGAATTCCCTTCGCCTGATGAATTGAAGTATCGGATCATTATTTCGACAAAACCTCCTAAAGAATACCTCAGGTCCAAAAATGTGAAAGGGGAGAGTTCTTTCACTGATGATGACGAGACTGATGAAGAAGAAGATGGCGATGATACGGTCTGCTTTGCGTTGAAATTTACCACATTTTAGCTGTGTTTTTCTAGTGATTTTCTGGTTCCAGTCTCTTGATGAAGTACACAACTCCATTGGGTTGCAGAGTGAGAGTGAGTCCAGCGAGAGTGATGACAGTGATTACGAATCTTACGCCACGGGTGTATCAGAATACAAACGCCTAATTGCCATTCATGCTGGAAAACCAAAGGGAGGTTTGAAGGAAGCTCTGAAAGTTGGAGCTGATAAGGTTAGACGCCTTAGCTTGAGTGAAAAAGCACTCAAAAAGGCAACTAAATTTCATGGCATGGAAGTCATTAGGTAACATAACACCTCAATTTACTCCAGCAAGAAATGAAATTGAGAGCTTCATTTTCCTCGTACTCACTTACTTTTTACAATGATTTTCCAAACTGACACCAAGCTTGCTCTGTTCTTGCTTCTAGATTCACCCAGAAGAACATCTTAAGGATATATCCGAAGGGAACTCGAGTAAATTCTTCCAACTACAAGCCACAGAATGCTTGGTTACACGGGGCACAGATGGTTGCTTTCAACATGCAGGTTTATTCTTTTCCTTTTGACACAAATCTGGGATCAAATAACAACGCTGGCCTTTTCTTCTGAAACATAAATTCAATTGAGCTATTGCAGGGACACGGAAAATCTCTCAGCTTAATGCGTGGCATGTTCCGATCCAACGGAGGTTGTGGTTATGTGAAAAAGCCCGACTTTCTACTGACGGTTGATCCCGATAGCAAGGTGTTTGATCCTAATGCAATCCTGCCAGTTAAAAGAACTTTGAAGGTAGCGTTTGCAAAACAAACTAGATCTTGTAGCATGTTCCGCTGGACCTGCTCTCAAAAACATTGTCTTGCAAATGACAGGTGAAAGTCTACATGGGTGTTGGCTGGAATTTGGATTTTGATAACACCCACTTTCATTTATACTCCCCTCCCGACTTCTATACACGGGTAAGTTTTAAATGAATGGCAATCTAGCTAAGCCAACTCCATAGCTTAATTGATATCAGTACCAAGAGATTATTCCATGCAAACTGCTAGAATTGCATACCGACCGTGTTAGCCATACGCAGGTTGGAATTGCAGGAGCTCCAGCTGATGAGATAATAAAGAAAACAAGGATCATAGAGGAGACTTGGAGCCCTGTTTGGAACGAAGAGTTCACATTTCCTTTGACTGTTCCTGAACTGGCGTTACTTCGAATCGAAGTGTATAGTTACAACATTTCGGATAAGGATGTTTTTGGAGGACAGAACTGTCTGCCCGTTCCTGAAGTGAGGCCAGGCATCCATGCTGTTCCTCTTTTTGACCGCAAAGGATCGAAGTACAGTTCTGTAAAGCTTCTAATGCGTTTTGAGTTTTCCTGA

mRNA sequence

ATGGGAAATTACAGGGTCTGTCTCTGCTTCATCAGAAGGTTCAAGATGTCCAAGGACGAGCCCCCCTCCGACGTCAAGGAAGCCTTCCTCAACTACGCCGGCGCCGCCGCCACCATGACCGCCGACCAGCTCCGCTCCTTTCTCCTCGACTTTCAGGGCGATCCCGCGCCCTCGCTCGCCGACGCTCACCGGATCATCGACCAAGTCCTGCACCGGCGCCACCACGTCGTGTCCAAGCTCACCAAGCACACTCTCACTCTTGACGATTTCTACTACTTCCTCTTCTCTGTGGATCTCAATCCCCCAATTTCCGATCAGGTCCATCAGGATATGAGAGCTCCATTGTCCCACTATTACGTTTACACTAGCCACAATACGTACCTGACTGGGAACCAATTGAGCAGTGATTCCAGCGATGTGCCGATTATCAATGCGCTGAAGAGCGGCGTGCGAGTCGTCGAGCTTGATTTGTGGCCCAACTCTGAGGAAGATGACATAGATGTTCTTCATGGAAGGACCTTGACAACACCTGTGGAATTTGTTAGATGTCTGAAATCAATTAAAGAGAATGCTTTCTTAGCATCTCCATATCCTGTAATAATTACTCTAGAAGACCACTTAACTCCTGATCTTCAAGCTAAAGCAGCTGAGAACATAAAGGAAACATTTGGGGACATGCTGTTCGTTCCTGAATCGGAATATCAACAAGAATTCCCTTCGCCTGATGAATTGAAGTATCGGATCATTATTTCGACAAAACCTCCTAAAGAATACCTCAGGTCCAAAAATGTGAAAGGGGAGAGTTCTTTCACTGATGATGACGAGACTGATGAAGAAGAAGATGGCGATGATACGAGTGAGAGTGAGTCCAGCGAGAGTGATGACAGTGATTACGAATCTTACGCCACGGGTGTATCAGAATACAAACGCCTAATTGCCATTCATGCTGGAAAACCAAAGGGAGGTTTGAAGGAAGCTCTGAAAGTTGGAGCTGATAAGGTTAGACGCCTTAGCTTGAGTGAAAAAGCACTCAAAAAGGCAACTAAATTTCATGGCATGGAAGTCATTAGATTCACCCAGAAGAACATCTTAAGGATATATCCGAAGGGAACTCGAGTAAATTCTTCCAACTACAAGCCACAGAATGCTTGGTTACACGGGGCACAGATGGTTGCTTTCAACATGCAGGGACACGGAAAATCTCTCAGCTTAATGCGTGGCATGTTCCGATCCAACGGAGGTTGTGGTTATGTGAAAAAGCCCGACTTTCTACTGACGGTTGATCCCGATAGCAAGGTGTTTGATCCTAATGCAATCCTGCCAGTTAAAAGAACTTTGAAGGTGAAAGTCTACATGGGTGTTGGCTGGAATTTGGATTTTGATAACACCCACTTTCATTTATACTCCCCTCCCGACTTCTATACACGGGTTGGAATTGCAGGAGCTCCAGCTGATGAGATAATAAAGAAAACAAGGATCATAGAGGAGACTTGGAGCCCTGTTTGGAACGAAGAGTTCACATTTCCTTTGACTGTTCCTGAACTGGCGTTACTTCGAATCGAAGTGTATAGTTACAACATTTCGGATAAGGATGTTTTTGGAGGACAGAACTGTCTGCCCGTTCCTGAAGTGAGGCCAGGCATCCATGCTGTTCCTCTTTTTGACCGCAAAGGATCGAAGTACAGTTCTGTAAAGCTTCTAATGCGTTTTGAGTTTTCCTGA

Coding sequence (CDS)

ATGGGAAATTACAGGGTCTGTCTCTGCTTCATCAGAAGGTTCAAGATGTCCAAGGACGAGCCCCCCTCCGACGTCAAGGAAGCCTTCCTCAACTACGCCGGCGCCGCCGCCACCATGACCGCCGACCAGCTCCGCTCCTTTCTCCTCGACTTTCAGGGCGATCCCGCGCCCTCGCTCGCCGACGCTCACCGGATCATCGACCAAGTCCTGCACCGGCGCCACCACGTCGTGTCCAAGCTCACCAAGCACACTCTCACTCTTGACGATTTCTACTACTTCCTCTTCTCTGTGGATCTCAATCCCCCAATTTCCGATCAGGTCCATCAGGATATGAGAGCTCCATTGTCCCACTATTACGTTTACACTAGCCACAATACGTACCTGACTGGGAACCAATTGAGCAGTGATTCCAGCGATGTGCCGATTATCAATGCGCTGAAGAGCGGCGTGCGAGTCGTCGAGCTTGATTTGTGGCCCAACTCTGAGGAAGATGACATAGATGTTCTTCATGGAAGGACCTTGACAACACCTGTGGAATTTGTTAGATGTCTGAAATCAATTAAAGAGAATGCTTTCTTAGCATCTCCATATCCTGTAATAATTACTCTAGAAGACCACTTAACTCCTGATCTTCAAGCTAAAGCAGCTGAGAACATAAAGGAAACATTTGGGGACATGCTGTTCGTTCCTGAATCGGAATATCAACAAGAATTCCCTTCGCCTGATGAATTGAAGTATCGGATCATTATTTCGACAAAACCTCCTAAAGAATACCTCAGGTCCAAAAATGTGAAAGGGGAGAGTTCTTTCACTGATGATGACGAGACTGATGAAGAAGAAGATGGCGATGATACGAGTGAGAGTGAGTCCAGCGAGAGTGATGACAGTGATTACGAATCTTACGCCACGGGTGTATCAGAATACAAACGCCTAATTGCCATTCATGCTGGAAAACCAAAGGGAGGTTTGAAGGAAGCTCTGAAAGTTGGAGCTGATAAGGTTAGACGCCTTAGCTTGAGTGAAAAAGCACTCAAAAAGGCAACTAAATTTCATGGCATGGAAGTCATTAGATTCACCCAGAAGAACATCTTAAGGATATATCCGAAGGGAACTCGAGTAAATTCTTCCAACTACAAGCCACAGAATGCTTGGTTACACGGGGCACAGATGGTTGCTTTCAACATGCAGGGACACGGAAAATCTCTCAGCTTAATGCGTGGCATGTTCCGATCCAACGGAGGTTGTGGTTATGTGAAAAAGCCCGACTTTCTACTGACGGTTGATCCCGATAGCAAGGTGTTTGATCCTAATGCAATCCTGCCAGTTAAAAGAACTTTGAAGGTGAAAGTCTACATGGGTGTTGGCTGGAATTTGGATTTTGATAACACCCACTTTCATTTATACTCCCCTCCCGACTTCTATACACGGGTTGGAATTGCAGGAGCTCCAGCTGATGAGATAATAAAGAAAACAAGGATCATAGAGGAGACTTGGAGCCCTGTTTGGAACGAAGAGTTCACATTTCCTTTGACTGTTCCTGAACTGGCGTTACTTCGAATCGAAGTGTATAGTTACAACATTTCGGATAAGGATGTTTTTGGAGGACAGAACTGTCTGCCCGTTCCTGAAGTGAGGCCAGGCATCCATGCTGTTCCTCTTTTTGACCGCAAAGGATCGAAGTACAGTTCTGTAAAGCTTCTAATGCGTTTTGAGTTTTCCTGA

Protein sequence

MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYVYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPSPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS
Homology
BLAST of Moc03g01710 vs. NCBI nr
Match: XP_022150957.1 (phosphoinositide phospholipase C 4-like isoform X1 [Momordica charantia])

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60
           MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA
Sbjct: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60

Query: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120
           DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV
Sbjct: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120

Query: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180
           YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF
Sbjct: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180

Query: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240
           VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS
Sbjct: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240

Query: 241 PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYES 300
           PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYES
Sbjct: 241 PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYES 300

Query: 301 YATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQ 360
           YATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQ
Sbjct: 301 YATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQ 360

Query: 361 KNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKK 420
           KNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKK
Sbjct: 361 KNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKK 420

Query: 421 PDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIA 480
           PDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIA
Sbjct: 421 PDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIA 480

Query: 481 GAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLP 540
           GAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLP
Sbjct: 481 GAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLP 540

Query: 541 VPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           VPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 VPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 573

BLAST of Moc03g01710 vs. NCBI nr
Match: XP_038894756.1 (phosphoinositide phospholipase C 4-like isoform X1 [Benincasa hispida])

HSP 1 Score: 1052.0 bits (2719), Expect = 1.9e-303
Identity = 520/574 (90.59%), Postives = 548/574 (95.47%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA GAAA MTA+QLRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGAAAHMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH+LTLDDFYY+LFS+DLNPPISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHSLTLDDFYYYLFSMDLNPPISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYE 300
           SP+ELKYRIIISTKPPKEYLRSKNVKGE+SF+ DDETD +EDG DTSESESSESDDSDYE
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSFS-DDETD-DEDGVDTSESESSESDDSDYE 300

Query: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFT 360
           SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATK HGM+VIRFT
Sbjct: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKSHGMDVIRFT 360

Query: 361 QKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420
           QKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNG CGYVK
Sbjct: 361 QKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGSCGYVK 420

Query: 421 KPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGI 480
           KPDFLLTVDP  KVFDPNA LPVKRTLKVKVYMGVGWNLDF++THFHLYSPPDFY R+GI
Sbjct: 421 KPDFLLTVDPHGKVFDPNATLPVKRTLKVKVYMGVGWNLDFESTHFHLYSPPDFYARIGI 480

Query: 481 AGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCL 540
           AGAPADEIIKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNISDKDV GGQNCL
Sbjct: 481 AGAPADEIIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVLGGQNCL 540

Query: 541 PVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           PVPE++PGIHAVPLFDRKG KYSSVKLLMRFEFS
Sbjct: 541 PVPEMKPGIHAVPLFDRKGLKYSSVKLLMRFEFS 572

BLAST of Moc03g01710 vs. NCBI nr
Match: XP_008457370.1 (PREDICTED: phosphoinositide phospholipase C 4-like isoform X1 [Cucumis melo])

HSP 1 Score: 1046.6 bits (2705), Expect = 7.9e-302
Identity = 515/574 (89.72%), Postives = 547/574 (95.30%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA G AA MTA+QLRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH+LTLDDFYY+LFS+DLNPPISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHSLTLDDFYYYLFSMDLNPPISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSS+SSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSNSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYE 300
           SP+ELKYRI+ISTKPPKEYLRSKNVKGE+SF+ DDETD +EDG DTSESESSESDDSDYE
Sbjct: 241 SPEELKYRIVISTKPPKEYLRSKNVKGENSFS-DDETD-DEDGVDTSESESSESDDSDYE 300

Query: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFT 360
           SY TGVSEYKRLIAIHAGKP GGLKEALKVGADKVRRLSLSEKALKKATK HGM+VIRFT
Sbjct: 301 SYTTGVSEYKRLIAIHAGKPNGGLKEALKVGADKVRRLSLSEKALKKATKAHGMDVIRFT 360

Query: 361 QKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420
           QKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK
Sbjct: 361 QKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420

Query: 421 KPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGI 480
           KPDFL+TVD   KVFDPNA LPVKRTLKVK+YMGVGWNLDF+NTHFHLYSPPDFYTR+GI
Sbjct: 421 KPDFLITVDSHGKVFDPNANLPVKRTLKVKLYMGVGWNLDFENTHFHLYSPPDFYTRIGI 480

Query: 481 AGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCL 540
           AGAPADE+IKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNISDKDVFGGQNCL
Sbjct: 481 AGAPADEVIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVFGGQNCL 540

Query: 541 PVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           PV EV+PGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 PVSEVKPGIHAVPLFDRKGSKYSSVKLLMRFEFS 572

BLAST of Moc03g01710 vs. NCBI nr
Match: XP_038894757.1 (phosphoinositide phospholipase C 4-like isoform X2 [Benincasa hispida])

HSP 1 Score: 1043.1 bits (2696), Expect = 8.8e-301
Identity = 520/586 (88.74%), Postives = 548/586 (93.52%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA GAAA MTA+QLRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGAAAHMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH+LTLDDFYY+LFS+DLNPPISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHSLTLDDFYYYLFSMDLNPPISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDT------------SE 300
           SP+ELKYRIIISTKPPKEYLRSKNVKGE+SF+ DDETD +EDG DT            SE
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGENSFS-DDETD-DEDGVDTYLDEVYNFIGLQSE 300

Query: 301 SESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKA 360
           SESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKA
Sbjct: 301 SESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKA 360

Query: 361 TKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRG 420
           TK HGM+VIRFTQKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRG
Sbjct: 361 TKSHGMDVIRFTQKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRG 420

Query: 421 MFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHL 480
           MFRSNG CGYVKKPDFLLTVDP  KVFDPNA LPVKRTLKVKVYMGVGWNLDF++THFHL
Sbjct: 421 MFRSNGSCGYVKKPDFLLTVDPHGKVFDPNATLPVKRTLKVKVYMGVGWNLDFESTHFHL 480

Query: 481 YSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNI 540
           YSPPDFY R+GIAGAPADEIIKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNI
Sbjct: 481 YSPPDFYARIGIAGAPADEIIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNI 540

Query: 541 SDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           SDKDV GGQNCLPVPE++PGIHAVPLFDRKG KYSSVKLLMRFEFS
Sbjct: 541 SDKDVLGGQNCLPVPEMKPGIHAVPLFDRKGLKYSSVKLLMRFEFS 584

BLAST of Moc03g01710 vs. NCBI nr
Match: XP_011658609.1 (phosphoinositide phospholipase C 4 isoform X1 [Cucumis sativus])

HSP 1 Score: 1039.6 bits (2687), Expect = 9.7e-300
Identity = 514/574 (89.55%), Postives = 543/574 (94.60%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA G AA MTA+ LRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEHLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH LTLDDFYY+LFS+DLNP ISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHCLTLDDFYYYLFSMDLNPSISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYE 300
           SP+ELKYRIIISTKPPKEYL+SKNVKGE+SF+ DDETD +EDG DTSESESSESDDSDYE
Sbjct: 241 SPEELKYRIIISTKPPKEYLKSKNVKGENSFS-DDETD-DEDGVDTSESESSESDDSDYE 300

Query: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFT 360
           SY TGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATK HG +VIRFT
Sbjct: 301 SYTTGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKAHGKDVIRFT 360

Query: 361 QKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420
           QKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK
Sbjct: 361 QKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420

Query: 421 KPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGI 480
           KPDFLLTVD   KVFDP A LPVKRTLKVKVYMGVGWNLDF+NTHFHLYSPPDFYT++GI
Sbjct: 421 KPDFLLTVDAHGKVFDPTANLPVKRTLKVKVYMGVGWNLDFENTHFHLYSPPDFYTKIGI 480

Query: 481 AGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCL 540
           AGAPADE+IKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNISDKDVFGGQNCL
Sbjct: 481 AGAPADELIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVFGGQNCL 540

Query: 541 PVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           PV EV+PGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 PVSEVKPGIHAVPLFDRKGSKYSSVKLLMRFEFS 572

BLAST of Moc03g01710 vs. ExPASy Swiss-Prot
Match: Q944C1 (Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 SV=2)

HSP 1 Score: 685.3 bits (1767), Expect = 6.1e-196
Identity = 345/592 (58.28%), Postives = 439/592 (74.16%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60
           MG+Y+ CL F R+F+M++  P  DV++ F  Y    A M+ +QL+  + +  G+   SL 
Sbjct: 7   MGSYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLE 66

Query: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120
           +A RI+D+VL R+HH ++K T+  LTLDDF Y+LFS DLNPPI+DQVHQ+M APLSHY++
Sbjct: 67  EAERIVDEVLRRKHH-IAKFTRRNLTLDDFNYYLFSTDLNPPIADQVHQNMDAPLSHYFI 126

Query: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180
           +T HN+YLTGNQLSS+ S++PI +AL+ GVRVVELDLWP    DD+ V HGRTLT  V+ 
Sbjct: 127 FTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRG-TDDVCVKHGRTLTKEVKL 186

Query: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240
            +CL+SIK NAF  S YPVIITLEDHLTP LQ K A+ I +TFGDML+  +S+  QEFPS
Sbjct: 187 GKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFPS 246

Query: 241 PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSES------- 300
           P+ELK +I+ISTKPPKEYL + + K + +     ++DE+  G +  +  S++S       
Sbjct: 247 PEELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDEDVWGKEPEDLISTQSDLDKVTS 306

Query: 301 -------DDSDYESYATGVS------EYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLS 360
                  DD +  S  +  S      EYKRLIAIHAGKPKGGL+ ALKV  +K+RRLSLS
Sbjct: 307 SVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRMALKVDPNKIRRLSLS 366

Query: 361 EKALKKATKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGK 420
           E+ L+KA   +G +VIRFTQKN LRIYPKGTR NSSNYKPQ  W+ GAQM+AFNMQG+G+
Sbjct: 367 EQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGYGR 426

Query: 421 SLSLMRGMFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDF 480
           +L LM GMFR+NGGCGYVKKPDFL+   P+ + F P      K+TLKVKV MG GW LDF
Sbjct: 427 ALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLLDF 486

Query: 481 DNTHFHLYSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRI 540
             THF  YSPPDF+ RVGIAGAP DE+++KT+I  +TW+P+WN+EFTFPL VPELALLR+
Sbjct: 487 KKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALLRV 546

Query: 541 EVYSYNISDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           EV+ +++++KD FGGQ CLPV E+R GI AVPLF+RKG KYSS +LLMRFEF
Sbjct: 547 EVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRFEF 596

BLAST of Moc03g01710 vs. ExPASy Swiss-Prot
Match: Q8GV43 (Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 SV=2)

HSP 1 Score: 683.3 bits (1762), Expect = 2.3e-195
Identity = 355/598 (59.36%), Postives = 435/598 (72.74%), Query Frame = 0

Query: 3   NYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA----------------GAAATMTADQLRS 62
           NYR+   + R+FK+++  P  DV++AF  +A                G+   M A+QL S
Sbjct: 18  NYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCS 77

Query: 63  FLLDFQGDPAPSLADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQ 122
           F LD  G+ + ++A+A R+ID+V+ RRHH V++ T+H L LDDF+ FLF  DLNPPI+  
Sbjct: 78  F-LDDHGE-STTVAEAQRLIDEVIRRRHH-VTRFTRHGLDLDDFFNFLFYDDLNPPITPH 137

Query: 123 VHQDMRAPLSHYYVYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDI 182
           VHQDM APLSHY++YT HN+YLTGNQLSSD S+VP+I AL+ GVRV+ELDLWPNS   DI
Sbjct: 138 VHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDI 197

Query: 183 DVLHGRTLTTPVEFVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDM 242
           +VLHGRTLTTPV  ++CLKSI++ AF +SPYPVIITLEDHLTPDLQAK AE   + FG M
Sbjct: 198 NVLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQM 257

Query: 243 LFVPESEYQQEFPSPDELKYRIIISTKPPKEYLRSKN--VKGES---SFTDDDETDEEED 302
           L+ PES+   EFPSP  L +RIIISTKPPKEYL S+N  VK +    S + +DET   E+
Sbjct: 258 LYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRTEE 317

Query: 303 GD-------DTSESESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKV 362
                    D      S+SD  D E+       YKRLI IHAGKPKG +KE +KV  DKV
Sbjct: 318 IQTLESMLFDQDFESKSDSDQEDEEASEDQKPAYKRLITIHAGKPKGTVKEEMKVVVDKV 377

Query: 363 RRLSLSEKALKKATKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFN 422
           RRLSLSE+ L +    +  +V+RFTQ+N+LRIYPKGTR NSSNYKP   W HGAQM+AFN
Sbjct: 378 RRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQMIAFN 437

Query: 423 MQGHGKSLSLMRGMFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGV 482
           MQG+GKSL LM GMFR+NGGCGYVKKP+FL+      +VFDP   LPVK TLKVKVYMG 
Sbjct: 438 MQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKETLKVKVYMGD 497

Query: 483 GWNLDFDNTHFHLYSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPE 542
           GW +DF +THF  YSPPDFYT++ I G PAD   KKT+IIE+ W P+W+EEF+FPLTVPE
Sbjct: 498 GWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFSFPLTVPE 557

Query: 543 LALLRIEVYSYNISDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           LALLRIEV  Y++S+KD FGGQ CLPV E+RPGI +VPL+D+KG K  SV+LLMRF F
Sbjct: 558 LALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKMKSVRLLMRFIF 612

BLAST of Moc03g01710 vs. ExPASy Swiss-Prot
Match: Q944C2 (Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 SV=2)

HSP 1 Score: 664.5 bits (1713), Expect = 1.1e-189
Identity = 332/575 (57.74%), Postives = 430/575 (74.78%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60
           MG+Y++ LC   + +M++  PP DV  AF+ Y    + MTA+QL  FL++ Q +    ++
Sbjct: 5   MGSYKMGLCCSDKLRMNRGAPPQDVVTAFVEYTEGRSHMTAEQLCRFLVEVQDETEVLVS 64

Query: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120
           DA +II+++   RHH ++K  +HTL LDDF+ FLFS DLN PI  +VHQDM +PLSHY++
Sbjct: 65  DAEKIIERITCERHH-ITKFLRHTLNLDDFFSFLFSDDLNHPIDSKVHQDMASPLSHYFI 124

Query: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180
           YTSHN+YLTGNQ++S+ SDVP+I ALK GVR +ELD+WPNS +DDI VLHG   T PVE 
Sbjct: 125 YTSHNSYLTGNQINSECSDVPLIKALKRGVRALELDMWPNSTKDDILVLHGWAWTPPVEL 184

Query: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240
           V+CL+SIKE+AF AS YPVI+TLEDHLTPDLQAKAAE +KE F DM++ PE+   +EFPS
Sbjct: 185 VKCLRSIKEHAFYASAYPVILTLEDHLTPDLQAKAAEMMKEIFMDMVYFPEAGGLKEFPS 244

Query: 241 PDELKYRIIISTKPPKEYLR-SKNVKGESS--FTDDDETDEEEDGDDTSESESSESDDSD 300
           P++LKY+I+ISTKPPK  LR  K+ + ++S   + D   D+E+  ++TSE++ +E D  D
Sbjct: 245 PEDLKYKIVISTKPPKGSLRKDKDSESDASGKASSDVSADDEKTEEETSEAK-NEEDGFD 304

Query: 301 YESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIR 360
            ES       Y RLI I +G  K GLKEAL +    VRRLSL E+  KKAT+ +G EVI+
Sbjct: 305 QESSNLDFLTYSRLITIPSGNAKNGLKEALTIDNGGVRRLSLREQKFKKATEMYGTEVIK 364

Query: 361 FTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGY 420
           FTQKN+LRIYPK TRVNSSNY+P N W++GAQMVAFNMQG+G++L +M GMFR NGGCGY
Sbjct: 365 FTQKNLLRIYPKATRVNSSNYRPYNGWMYGAQMVAFNMQGYGRALWMMHGMFRGNGGCGY 424

Query: 421 VKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRV 480
           VKKPDF++  +   +VF+P A LP+K+TLKVKVYMG GW+  F  T F+ +S P+FYTRV
Sbjct: 425 VKKPDFMMNNNLSGEVFNPKAKLPIKKTLKVKVYMGKGWDSGFQRTCFNTWSSPNFYTRV 484

Query: 481 GIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQN 540
           GI G   D+++KKT+  ++TW P WNEEF F LTVPELALLRIEV+ YN+ +KD F GQ 
Sbjct: 485 GITGVRGDKVMKKTKKEQKTWEPFWNEEFEFQLTVPELALLRIEVHDYNMPEKDDFSGQT 544

Query: 541 CLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           CLPV E+R GI +VPL+DRKG +  SV LLMRF F
Sbjct: 545 CLPVSELRQGIRSVPLYDRKGERLVSVTLLMRFHF 577

BLAST of Moc03g01710 vs. ExPASy Swiss-Prot
Match: Q39033 (Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 SV=1)

HSP 1 Score: 645.2 bits (1663), Expect = 7.0e-184
Identity = 331/584 (56.68%), Postives = 412/584 (70.55%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLADAH 63
           Y+VC CF RRF+ +  E P ++K  F  Y+     MT D L  FL+D Q     +  DA 
Sbjct: 6   YKVCFCFRRRFRYTASEAPREIKTIFEKYS-ENGVMTVDHLHRFLIDVQKQDKATREDAQ 65

Query: 64  RIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPIS-DQVHQDMRAPLSHYYVYT 123
            II+          S L ++ L LD F+ +LF  D NPP++  +VH DM AP+SHY+++T
Sbjct: 66  SIINS-------ASSLLHRNGLHLDAFFKYLFG-DNNPPLALHKVHHDMDAPISHYFIFT 125

Query: 124 SHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVR 183
            HN+YLTGNQLSSD S+VPII+ALK GVRV+ELD+WPNS +DDIDVLHG TLTTPV  ++
Sbjct: 126 GHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIK 185

Query: 184 CLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPE-SEYQQEFPSP 243
           CLK+I+ +AF  S YPV++TLEDHLTPDLQ+K AE + E FG++LF P   E  +EFPSP
Sbjct: 186 CLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSP 245

Query: 244 DELKYRIIISTKPPKEYLRSKNVKGESSFTD--DDET-----------DEEEDGDDTSES 303
           + LK RIIISTKPPKEY   K+V+      D  D+E            ++ E  DD   +
Sbjct: 246 NSLKRRIIISTKPPKEYKEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLDGN 305

Query: 304 ESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKAT 363
           +  + DD + +S      +YK LIAIHAGKPKGG+ E LKV  DKVRRLSLSE+ L+KA 
Sbjct: 306 DDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGITECLKVDPDKVRRLSLSEEQLEKAA 365

Query: 364 KFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGM 423
           + +  +++RFTQ N+LRIYPKGTRV SSNY P   W HGAQMVAFNMQG+G+SL LM+GM
Sbjct: 366 EKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLMQGM 425

Query: 424 FRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLY 483
           FR+NGGCGY+KKPD LL    DS +FDP A LPVK TL+V VYMG GW  DF +THF  Y
Sbjct: 426 FRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHFDQY 485

Query: 484 SPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNIS 543
           SPPDFYTRVGIAG P D ++KKT+ +E+ W P W+E F FPLTVPELALLR+EV+ Y++S
Sbjct: 486 SPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEYDMS 545

Query: 544 DKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           +KD FGGQ CLPV E+  GI A PL  RKG KY SVKLL++ EF
Sbjct: 546 EKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 580

BLAST of Moc03g01710 vs. ExPASy Swiss-Prot
Match: Q9LY51 (Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 6.8e-179
Identity = 330/589 (56.03%), Postives = 414/589 (70.29%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLADAH 63
           Y+VC CF RR++ +    P+++K  F NY+     MT D L  FL+D Q     +  +A 
Sbjct: 6   YKVCFCFRRRYRHTVSVAPAEIKTLFDNYSD-KGLMTTDLLLRFLIDVQKQDKATKEEAQ 65

Query: 64  RIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYVYTS 123
            I++          S L ++ L LD F+ +LF+V  +P  S +VHQDM APLSHY++YT 
Sbjct: 66  DIVNA-------SSSLLHRNGLHLDAFFKYLFAVTNSPLSSLEVHQDMDAPLSHYFIYTG 125

Query: 124 HNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVRC 183
           HN+YLTGNQLSSD S++PII ALK GVRV+ELD+WPNS+ED IDVLHGRTLT+PVE ++C
Sbjct: 126 HNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPVELIKC 185

Query: 184 LKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPES-EYQQEFPSPD 243
           L++I+E+AF  S YPV++TLEDHLTP LQAK AE + + FG+MLF P S E  +EFPSP 
Sbjct: 186 LRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFPSPA 245

Query: 244 ELKYRIIISTKPPKEY--------------LRSKNVKGES--SFTDDDETDEEEDG---D 303
            LK RI+ISTKPPKEY              L  K V G    SF   D + ++ D    D
Sbjct: 246 FLKKRIMISTKPPKEYKAATDDDLVKKGRDLGDKEVWGREVPSFIRRDRSVDKNDSNGDD 305

Query: 304 DTSESESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKA 363
           D  + +  + DD D +       EYK LIAI AGKPKGG+ E LKV  DKVRRLSLSE+ 
Sbjct: 306 DDDDDDDDDDDDGDDKIKKNAPPEYKHLIAIEAGKPKGGITECLKVDPDKVRRLSLSEEQ 365

Query: 364 LKKATKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLS 423
           L+KA++ +  +++RFTQ+N+LR+YPKGTR+ SSNY P  AW HGAQMVAFNMQG G+SL 
Sbjct: 366 LEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQMVAFNMQGLGRSLW 425

Query: 424 LMRGMFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNT 483
           +M+GMFR NGGCGY+KKPD LL     + VFDP A LPVK TL+V +YMG GW  DF +T
Sbjct: 426 VMQGMFRGNGGCGYIKKPDLLL---KSNAVFDPEATLPVKTTLRVTIYMGEGWYYDFPHT 485

Query: 484 HFHLYSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVY 543
           HF  YSPPDFYTRVGIAG PAD ++KKT+ +E+ W P W+E F FPLTVPELALLRIEV+
Sbjct: 486 HFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRIEVH 545

Query: 544 SYNISDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
            Y++S+KD FGGQ CLPV E+R GI AVPL ++ G K  SVKLL+R EF
Sbjct: 546 EYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRLEF 583

BLAST of Moc03g01710 vs. ExPASy TrEMBL
Match: A0A6J1DC68 (Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111018983 PE=4 SV=1)

HSP 1 Score: 1157.5 bits (2993), Expect = 0.0e+00
Identity = 573/573 (100.00%), Postives = 573/573 (100.00%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60
           MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA
Sbjct: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60

Query: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120
           DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV
Sbjct: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120

Query: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180
           YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF
Sbjct: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180

Query: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240
           VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS
Sbjct: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240

Query: 241 PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYES 300
           PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYES
Sbjct: 241 PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYES 300

Query: 301 YATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQ 360
           YATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQ
Sbjct: 301 YATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFTQ 360

Query: 361 KNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKK 420
           KNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKK
Sbjct: 361 KNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVKK 420

Query: 421 PDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIA 480
           PDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIA
Sbjct: 421 PDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGIA 480

Query: 481 GAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLP 540
           GAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLP
Sbjct: 481 GAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCLP 540

Query: 541 VPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           VPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 VPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 573

BLAST of Moc03g01710 vs. ExPASy TrEMBL
Match: A0A1S3C6M6 (Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103497078 PE=4 SV=1)

HSP 1 Score: 1046.6 bits (2705), Expect = 3.8e-302
Identity = 515/574 (89.72%), Postives = 547/574 (95.30%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA G AA MTA+QLRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH+LTLDDFYY+LFS+DLNPPISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHSLTLDDFYYYLFSMDLNPPISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSS+SSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSNSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYE 300
           SP+ELKYRI+ISTKPPKEYLRSKNVKGE+SF+ DDETD +EDG DTSESESSESDDSDYE
Sbjct: 241 SPEELKYRIVISTKPPKEYLRSKNVKGENSFS-DDETD-DEDGVDTSESESSESDDSDYE 300

Query: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFT 360
           SY TGVSEYKRLIAIHAGKP GGLKEALKVGADKVRRLSLSEKALKKATK HGM+VIRFT
Sbjct: 301 SYTTGVSEYKRLIAIHAGKPNGGLKEALKVGADKVRRLSLSEKALKKATKAHGMDVIRFT 360

Query: 361 QKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420
           QKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK
Sbjct: 361 QKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420

Query: 421 KPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGI 480
           KPDFL+TVD   KVFDPNA LPVKRTLKVK+YMGVGWNLDF+NTHFHLYSPPDFYTR+GI
Sbjct: 421 KPDFLITVDSHGKVFDPNANLPVKRTLKVKLYMGVGWNLDFENTHFHLYSPPDFYTRIGI 480

Query: 481 AGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCL 540
           AGAPADE+IKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNISDKDVFGGQNCL
Sbjct: 481 AGAPADEVIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVFGGQNCL 540

Query: 541 PVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           PV EV+PGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 PVSEVKPGIHAVPLFDRKGSKYSSVKLLMRFEFS 572

BLAST of Moc03g01710 vs. ExPASy TrEMBL
Match: A0A0A0LVI7 (Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_1G531180 PE=4 SV=1)

HSP 1 Score: 1039.6 bits (2687), Expect = 4.7e-300
Identity = 514/574 (89.55%), Postives = 543/574 (94.60%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA G AA MTA+ LRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEHLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH LTLDDFYY+LFS+DLNP ISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHCLTLDDFYYYLFSMDLNPSISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYE 300
           SP+ELKYRIIISTKPPKEYL+SKNVKGE+SF+ DDETD +EDG DTSESESSESDDSDYE
Sbjct: 241 SPEELKYRIIISTKPPKEYLKSKNVKGENSFS-DDETD-DEDGVDTSESESSESDDSDYE 300

Query: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFT 360
           SY TGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATK HG +VIRFT
Sbjct: 301 SYTTGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKAHGKDVIRFT 360

Query: 361 QKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420
           QKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK
Sbjct: 361 QKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420

Query: 421 KPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGI 480
           KPDFLLTVD   KVFDP A LPVKRTLKVKVYMGVGWNLDF+NTHFHLYSPPDFYT++GI
Sbjct: 421 KPDFLLTVDAHGKVFDPTANLPVKRTLKVKVYMGVGWNLDFENTHFHLYSPPDFYTKIGI 480

Query: 481 AGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCL 540
           AGAPADE+IKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNISDKDVFGGQNCL
Sbjct: 481 AGAPADELIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKDVFGGQNCL 540

Query: 541 PVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           PV EV+PGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 PVSEVKPGIHAVPLFDRKGSKYSSVKLLMRFEFS 572

BLAST of Moc03g01710 vs. ExPASy TrEMBL
Match: A0A6J1HKD4 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111464902 PE=4 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.3e-299
Identity = 507/574 (88.33%), Postives = 547/574 (95.30%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNY-AGAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP +VK+AF+ Y  G +A MTA+QLRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPEVKDAFIKYTGGTSANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV+SKLT+HTLTLDDFYY+LFS+DLNPP+SDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVISKLTRHTLTLDDFYYYLFSMDLNPPVSDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSE+DDIDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEQDDIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSESDDSDYE 300
           SP+ELKYRIIISTKPPKEYLRSKNVKGE+SF+ DDETD ++DG DTSESES+ESDDSDYE
Sbjct: 241 SPEELKYRIIISTKPPKEYLRSKNVKGETSFS-DDETD-DDDGVDTSESESTESDDSDYE 300

Query: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIRFT 360
           SYATGVSEYKRLIAIHAGKPKGGLKEALKVGA KVRRLSLSEKA+KKATK HGM+VIRFT
Sbjct: 301 SYATGVSEYKRLIAIHAGKPKGGLKEALKVGAAKVRRLSLSEKAVKKATKSHGMDVIRFT 360

Query: 361 QKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGYVK 420
           Q N+LRIYPKGTRVNSSNYKPQ AWLHGAQMVAFNMQGHG+SLSLMRGMFRSNGGCGYVK
Sbjct: 361 QTNMLRIYPKGTRVNSSNYKPQTAWLHGAQMVAFNMQGHGRSLSLMRGMFRSNGGCGYVK 420

Query: 421 KPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRVGI 480
           KPDFLLTVDP  KVFDPNA LPVK+T+KVK+YMGVGWNLDF+ +HFHLYSPPDFYTR+GI
Sbjct: 421 KPDFLLTVDPHGKVFDPNANLPVKKTMKVKIYMGVGWNLDFERSHFHLYSPPDFYTRIGI 480

Query: 481 AGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQNCL 540
           AGAPADEIIKKT I EETWSPVWN+EF+FPLT+PELALLRIEVYSYNISDK+VFGGQNCL
Sbjct: 481 AGAPADEIIKKTDIKEETWSPVWNKEFSFPLTIPELALLRIEVYSYNISDKNVFGGQNCL 540

Query: 541 PVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           PVPEV+PGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 PVPEVKPGIHAVPLFDRKGSKYSSVKLLMRFEFS 572

BLAST of Moc03g01710 vs. ExPASy TrEMBL
Match: A0A1S3C4X8 (Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103497078 PE=4 SV=1)

HSP 1 Score: 1035.4 bits (2676), Expect = 8.9e-299
Identity = 512/582 (87.97%), Postives = 545/582 (93.64%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA-GAAATMTADQLRSFLLDFQGDPAPSL 60
           MGNYRVCLCFIRRFKMSK+EPP DVKEAF+ YA G AA MTA+QLRSFL++FQGD   SL
Sbjct: 1   MGNYRVCLCFIRRFKMSKEEPPPDVKEAFVKYAGGVAANMTAEQLRSFLVEFQGDQTASL 60

Query: 61  ADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYY 120
           ADA RI++ VL RRHHV++KLTKH+LTLDDFYY+LFS+DLNPPISDQVHQDMRAPLSHYY
Sbjct: 61  ADAQRIVEHVLQRRHHVITKLTKHSLTLDDFYYYLFSMDLNPPISDQVHQDMRAPLSHYY 120

Query: 121 VYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVE 180
           +YTSHNTYLTGNQLSS+SSDVPIINALKSGVRVVELDLWPNSEED+IDVLHGRTLTTPVE
Sbjct: 121 IYTSHNTYLTGNQLSSNSSDVPIINALKSGVRVVELDLWPNSEEDEIDVLHGRTLTTPVE 180

Query: 181 FVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFP 240
           FVRCLKSIKENAF+ASPYPVIITLEDHLTPDLQAKAAE IKETFGDMLF PE+EY QEFP
Sbjct: 181 FVRCLKSIKENAFIASPYPVIITLEDHLTPDLQAKAAEYIKETFGDMLFFPEAEYLQEFP 240

Query: 241 SPDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDD--------TSESESS 300
           SP+ELKYRI+ISTKPPKEYLRSKNVKGE+SF+ DDETD+E+   D         SESESS
Sbjct: 241 SPEELKYRIVISTKPPKEYLRSKNVKGENSFS-DDETDDEDGYLDGVYNFVGLQSESESS 300

Query: 301 ESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFH 360
           ESDDSDYESY TGVSEYKRLIAIHAGKP GGLKEALKVGADKVRRLSLSEKALKKATK H
Sbjct: 301 ESDDSDYESYTTGVSEYKRLIAIHAGKPNGGLKEALKVGADKVRRLSLSEKALKKATKAH 360

Query: 361 GMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRS 420
           GM+VIRFTQKN+LRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRS
Sbjct: 361 GMDVIRFTQKNMLRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRS 420

Query: 421 NGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPP 480
           NGGCGYVKKPDFL+TVD   KVFDPNA LPVKRTLKVK+YMGVGWNLDF+NTHFHLYSPP
Sbjct: 421 NGGCGYVKKPDFLITVDSHGKVFDPNANLPVKRTLKVKLYMGVGWNLDFENTHFHLYSPP 480

Query: 481 DFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKD 540
           DFYTR+GIAGAPADE+IKKT I EETWSPVWNEEFTFPLT+PELALLRI+VYSYNISDKD
Sbjct: 481 DFYTRIGIAGAPADEVIKKTEIKEETWSPVWNEEFTFPLTIPELALLRIDVYSYNISDKD 540

Query: 541 VFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEFS 574
           VFGGQNCLPV EV+PGIHAVPLFDRKGSKYSSVKLLMRFEFS
Sbjct: 541 VFGGQNCLPVSEVKPGIHAVPLFDRKGSKYSSVKLLMRFEFS 581

BLAST of Moc03g01710 vs. TAIR 10
Match: AT5G58700.1 (phosphatidylinositol-speciwc phospholipase C4 )

HSP 1 Score: 685.3 bits (1767), Expect = 4.3e-197
Identity = 345/592 (58.28%), Postives = 439/592 (74.16%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60
           MG+Y+ CL F R+F+M++  P  DV++ F  Y    A M+ +QL+  + +  G+   SL 
Sbjct: 7   MGSYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLE 66

Query: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120
           +A RI+D+VL R+HH ++K T+  LTLDDF Y+LFS DLNPPI+DQVHQ+M APLSHY++
Sbjct: 67  EAERIVDEVLRRKHH-IAKFTRRNLTLDDFNYYLFSTDLNPPIADQVHQNMDAPLSHYFI 126

Query: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180
           +T HN+YLTGNQLSS+ S++PI +AL+ GVRVVELDLWP    DD+ V HGRTLT  V+ 
Sbjct: 127 FTGHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRG-TDDVCVKHGRTLTKEVKL 186

Query: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240
            +CL+SIK NAF  S YPVIITLEDHLTP LQ K A+ I +TFGDML+  +S+  QEFPS
Sbjct: 187 GKCLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFPS 246

Query: 241 PDELKYRIIISTKPPKEYLRSKNVKGESSFTDDDETDEEEDGDDTSESESSES------- 300
           P+ELK +I+ISTKPPKEYL + + K + +     ++DE+  G +  +  S++S       
Sbjct: 247 PEELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDEDVWGKEPEDLISTQSDLDKVTS 306

Query: 301 -------DDSDYESYATGVS------EYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLS 360
                  DD +  S  +  S      EYKRLIAIHAGKPKGGL+ ALKV  +K+RRLSLS
Sbjct: 307 SVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRMALKVDPNKIRRLSLS 366

Query: 361 EKALKKATKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGK 420
           E+ L+KA   +G +VIRFTQKN LRIYPKGTR NSSNYKPQ  W+ GAQM+AFNMQG+G+
Sbjct: 367 EQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIAFNMQGYGR 426

Query: 421 SLSLMRGMFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDF 480
           +L LM GMFR+NGGCGYVKKPDFL+   P+ + F P      K+TLKVKV MG GW LDF
Sbjct: 427 ALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCMGDGWLLDF 486

Query: 481 DNTHFHLYSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRI 540
             THF  YSPPDF+ RVGIAGAP DE+++KT+I  +TW+P+WN+EFTFPL VPELALLR+
Sbjct: 487 KKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAVPELALLRV 546

Query: 541 EVYSYNISDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           EV+ +++++KD FGGQ CLPV E+R GI AVPLF+RKG KYSS +LLMRFEF
Sbjct: 547 EVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRFEF 596

BLAST of Moc03g01710 vs. TAIR 10
Match: AT2G40116.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 683.3 bits (1762), Expect = 1.6e-196
Identity = 355/598 (59.36%), Postives = 435/598 (72.74%), Query Frame = 0

Query: 3   NYRVCLCFIRRFKMSKDEPPSDVKEAFLNYA----------------GAAATMTADQLRS 62
           NYR+   + R+FK+++  P  DV++AF  +A                G+   M A+QL S
Sbjct: 18  NYRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCS 77

Query: 63  FLLDFQGDPAPSLADAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQ 122
           F LD  G+ + ++A+A R+ID+V+ RRHH V++ T+H L LDDF+ FLF  DLNPPI+  
Sbjct: 78  F-LDDHGE-STTVAEAQRLIDEVIRRRHH-VTRFTRHGLDLDDFFNFLFYDDLNPPITPH 137

Query: 123 VHQDMRAPLSHYYVYTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDI 182
           VHQDM APLSHY++YT HN+YLTGNQLSSD S+VP+I AL+ GVRV+ELDLWPNS   DI
Sbjct: 138 VHQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDI 197

Query: 183 DVLHGRTLTTPVEFVRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDM 242
           +VLHGRTLTTPV  ++CLKSI++ AF +SPYPVIITLEDHLTPDLQAK AE   + FG M
Sbjct: 198 NVLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQM 257

Query: 243 LFVPESEYQQEFPSPDELKYRIIISTKPPKEYLRSKN--VKGES---SFTDDDETDEEED 302
           L+ PES+   EFPSP  L +RIIISTKPPKEYL S+N  VK +    S + +DET   E+
Sbjct: 258 LYYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRTEE 317

Query: 303 GD-------DTSESESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKV 362
                    D      S+SD  D E+       YKRLI IHAGKPKG +KE +KV  DKV
Sbjct: 318 IQTLESMLFDQDFESKSDSDQEDEEASEDQKPAYKRLITIHAGKPKGTVKEEMKVVVDKV 377

Query: 363 RRLSLSEKALKKATKFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFN 422
           RRLSLSE+ L +    +  +V+RFTQ+N+LRIYPKGTR NSSNYKP   W HGAQM+AFN
Sbjct: 378 RRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPLIGWTHGAQMIAFN 437

Query: 423 MQGHGKSLSLMRGMFRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGV 482
           MQG+GKSL LM GMFR+NGGCGYVKKP+FL+      +VFDP   LPVK TLKVKVYMG 
Sbjct: 438 MQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLPVKETLKVKVYMGD 497

Query: 483 GWNLDFDNTHFHLYSPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPE 542
           GW +DF +THF  YSPPDFYT++ I G PAD   KKT+IIE+ W P+W+EEF+FPLTVPE
Sbjct: 498 GWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPIWDEEFSFPLTVPE 557

Query: 543 LALLRIEVYSYNISDKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           LALLRIEV  Y++S+KD FGGQ CLPV E+RPGI +VPL+D+KG K  SV+LLMRF F
Sbjct: 558 LALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKMKSVRLLMRFIF 612

BLAST of Moc03g01710 vs. TAIR 10
Match: AT5G58690.1 (phosphatidylinositol-speciwc phospholipase C5 )

HSP 1 Score: 664.5 bits (1713), Expect = 7.9e-191
Identity = 332/575 (57.74%), Postives = 430/575 (74.78%), Query Frame = 0

Query: 1   MGNYRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLA 60
           MG+Y++ LC   + +M++  PP DV  AF+ Y    + MTA+QL  FL++ Q +    ++
Sbjct: 5   MGSYKMGLCCSDKLRMNRGAPPQDVVTAFVEYTEGRSHMTAEQLCRFLVEVQDETEVLVS 64

Query: 61  DAHRIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPISDQVHQDMRAPLSHYYV 120
           DA +II+++   RHH ++K  +HTL LDDF+ FLFS DLN PI  +VHQDM +PLSHY++
Sbjct: 65  DAEKIIERITCERHH-ITKFLRHTLNLDDFFSFLFSDDLNHPIDSKVHQDMASPLSHYFI 124

Query: 121 YTSHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEF 180
           YTSHN+YLTGNQ++S+ SDVP+I ALK GVR +ELD+WPNS +DDI VLHG   T PVE 
Sbjct: 125 YTSHNSYLTGNQINSECSDVPLIKALKRGVRALELDMWPNSTKDDILVLHGWAWTPPVEL 184

Query: 181 VRCLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPESEYQQEFPS 240
           V+CL+SIKE+AF AS YPVI+TLEDHLTPDLQAKAAE +KE F DM++ PE+   +EFPS
Sbjct: 185 VKCLRSIKEHAFYASAYPVILTLEDHLTPDLQAKAAEMMKEIFMDMVYFPEAGGLKEFPS 244

Query: 241 PDELKYRIIISTKPPKEYLR-SKNVKGESS--FTDDDETDEEEDGDDTSESESSESDDSD 300
           P++LKY+I+ISTKPPK  LR  K+ + ++S   + D   D+E+  ++TSE++ +E D  D
Sbjct: 245 PEDLKYKIVISTKPPKGSLRKDKDSESDASGKASSDVSADDEKTEEETSEAK-NEEDGFD 304

Query: 301 YESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKATKFHGMEVIR 360
            ES       Y RLI I +G  K GLKEAL +    VRRLSL E+  KKAT+ +G EVI+
Sbjct: 305 QESSNLDFLTYSRLITIPSGNAKNGLKEALTIDNGGVRRLSLREQKFKKATEMYGTEVIK 364

Query: 361 FTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGMFRSNGGCGY 420
           FTQKN+LRIYPK TRVNSSNY+P N W++GAQMVAFNMQG+G++L +M GMFR NGGCGY
Sbjct: 365 FTQKNLLRIYPKATRVNSSNYRPYNGWMYGAQMVAFNMQGYGRALWMMHGMFRGNGGCGY 424

Query: 421 VKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLYSPPDFYTRV 480
           VKKPDF++  +   +VF+P A LP+K+TLKVKVYMG GW+  F  T F+ +S P+FYTRV
Sbjct: 425 VKKPDFMMNNNLSGEVFNPKAKLPIKKTLKVKVYMGKGWDSGFQRTCFNTWSSPNFYTRV 484

Query: 481 GIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNISDKDVFGGQN 540
           GI G   D+++KKT+  ++TW P WNEEF F LTVPELALLRIEV+ YN+ +KD F GQ 
Sbjct: 485 GITGVRGDKVMKKTKKEQKTWEPFWNEEFEFQLTVPELALLRIEVHDYNMPEKDDFSGQT 544

Query: 541 CLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           CLPV E+R GI +VPL+DRKG +  SV LLMRF F
Sbjct: 545 CLPVSELRQGIRSVPLYDRKGERLVSVTLLMRFHF 577

BLAST of Moc03g01710 vs. TAIR 10
Match: AT3G08510.1 (phospholipase C 2 )

HSP 1 Score: 645.2 bits (1663), Expect = 5.0e-185
Identity = 331/584 (56.68%), Postives = 412/584 (70.55%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLADAH 63
           Y+VC CF RRF+ +  E P ++K  F  Y+     MT D L  FL+D Q     +  DA 
Sbjct: 6   YKVCFCFRRRFRYTASEAPREIKTIFEKYS-ENGVMTVDHLHRFLIDVQKQDKATREDAQ 65

Query: 64  RIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPIS-DQVHQDMRAPLSHYYVYT 123
            II+          S L ++ L LD F+ +LF  D NPP++  +VH DM AP+SHY+++T
Sbjct: 66  SIINS-------ASSLLHRNGLHLDAFFKYLFG-DNNPPLALHKVHHDMDAPISHYFIFT 125

Query: 124 SHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVR 183
            HN+YLTGNQLSSD S+VPII+ALK GVRV+ELD+WPNS +DDIDVLHG TLTTPV  ++
Sbjct: 126 GHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIK 185

Query: 184 CLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPE-SEYQQEFPSP 243
           CLK+I+ +AF  S YPV++TLEDHLTPDLQ+K AE + E FG++LF P   E  +EFPSP
Sbjct: 186 CLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSP 245

Query: 244 DELKYRIIISTKPPKEYLRSKNVKGESSFTD--DDET-----------DEEEDGDDTSES 303
           + LK RIIISTKPPKEY   K+V+      D  D+E            ++ E  DD   +
Sbjct: 246 NSLKRRIIISTKPPKEYKEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLDGN 305

Query: 304 ESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKAT 363
           +  + DD + +S      +YK LIAIHAGKPKGG+ E LKV  DKVRRLSLSE+ L+KA 
Sbjct: 306 DDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGITECLKVDPDKVRRLSLSEEQLEKAA 365

Query: 364 KFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGM 423
           + +  +++RFTQ N+LRIYPKGTRV SSNY P   W HGAQMVAFNMQG+G+SL LM+GM
Sbjct: 366 EKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLMQGM 425

Query: 424 FRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLY 483
           FR+NGGCGY+KKPD LL    DS +FDP A LPVK TL+V VYMG GW  DF +THF  Y
Sbjct: 426 FRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHFDQY 485

Query: 484 SPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNIS 543
           SPPDFYTRVGIAG P D ++KKT+ +E+ W P W+E F FPLTVPELALLR+EV+ Y++S
Sbjct: 486 SPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEYDMS 545

Query: 544 DKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           +KD FGGQ CLPV E+  GI A PL  RKG KY SVKLL++ EF
Sbjct: 546 EKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 580

BLAST of Moc03g01710 vs. TAIR 10
Match: AT3G08510.2 (phospholipase C 2 )

HSP 1 Score: 645.2 bits (1663), Expect = 5.0e-185
Identity = 331/584 (56.68%), Postives = 412/584 (70.55%), Query Frame = 0

Query: 4   YRVCLCFIRRFKMSKDEPPSDVKEAFLNYAGAAATMTADQLRSFLLDFQGDPAPSLADAH 63
           Y+VC CF RRF+ +  E P ++K  F  Y+     MT D L  FL+D Q     +  DA 
Sbjct: 6   YKVCFCFRRRFRYTASEAPREIKTIFEKYS-ENGVMTVDHLHRFLIDVQKQDKATREDAQ 65

Query: 64  RIIDQVLHRRHHVVSKLTKHTLTLDDFYYFLFSVDLNPPIS-DQVHQDMRAPLSHYYVYT 123
            II+          S L ++ L LD F+ +LF  D NPP++  +VH DM AP+SHY+++T
Sbjct: 66  SIINS-------ASSLLHRNGLHLDAFFKYLFG-DNNPPLALHKVHHDMDAPISHYFIFT 125

Query: 124 SHNTYLTGNQLSSDSSDVPIINALKSGVRVVELDLWPNSEEDDIDVLHGRTLTTPVEFVR 183
            HN+YLTGNQLSSD S+VPII+ALK GVRV+ELD+WPNS +DDIDVLHG TLTTPV  ++
Sbjct: 126 GHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGLIK 185

Query: 184 CLKSIKENAFLASPYPVIITLEDHLTPDLQAKAAENIKETFGDMLFVPE-SEYQQEFPSP 243
           CLK+I+ +AF  S YPV++TLEDHLTPDLQ+K AE + E FG++LF P   E  +EFPSP
Sbjct: 186 CLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFPSP 245

Query: 244 DELKYRIIISTKPPKEYLRSKNVKGESSFTD--DDET-----------DEEEDGDDTSES 303
           + LK RIIISTKPPKEY   K+V+      D  D+E            ++ E  DD   +
Sbjct: 246 NSLKRRIIISTKPPKEYKEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKSEAKDDLDGN 305

Query: 304 ESSESDDSDYESYATGVSEYKRLIAIHAGKPKGGLKEALKVGADKVRRLSLSEKALKKAT 363
           +  + DD + +S      +YK LIAIHAGKPKGG+ E LKV  DKVRRLSLSE+ L+KA 
Sbjct: 306 DDDDDDDDEDKSKINAPPQYKHLIAIHAGKPKGGITECLKVDPDKVRRLSLSEEQLEKAA 365

Query: 364 KFHGMEVIRFTQKNILRIYPKGTRVNSSNYKPQNAWLHGAQMVAFNMQGHGKSLSLMRGM 423
           + +  +++RFTQ N+LRIYPKGTRV SSNY P   W HGAQMVAFNMQG+G+SL LM+GM
Sbjct: 366 EKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFNMQGYGRSLWLMQGM 425

Query: 424 FRSNGGCGYVKKPDFLLTVDPDSKVFDPNAILPVKRTLKVKVYMGVGWNLDFDNTHFHLY 483
           FR+NGGCGY+KKPD LL    DS +FDP A LPVK TL+V VYMG GW  DF +THF  Y
Sbjct: 426 FRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGEGWYFDFRHTHFDQY 485

Query: 484 SPPDFYTRVGIAGAPADEIIKKTRIIEETWSPVWNEEFTFPLTVPELALLRIEVYSYNIS 543
           SPPDFYTRVGIAG P D ++KKT+ +E+ W P W+E F FPLTVPELALLR+EV+ Y++S
Sbjct: 486 SPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPELALLRLEVHEYDMS 545

Query: 544 DKDVFGGQNCLPVPEVRPGIHAVPLFDRKGSKYSSVKLLMRFEF 573
           +KD FGGQ CLPV E+  GI A PL  RKG KY SVKLL++ EF
Sbjct: 546 EKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEF 580

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150957.10.0e+00100.00phosphoinositide phospholipase C 4-like isoform X1 [Momordica charantia][more]
XP_038894756.11.9e-30390.59phosphoinositide phospholipase C 4-like isoform X1 [Benincasa hispida][more]
XP_008457370.17.9e-30289.72PREDICTED: phosphoinositide phospholipase C 4-like isoform X1 [Cucumis melo][more]
XP_038894757.18.8e-30188.74phosphoinositide phospholipase C 4-like isoform X2 [Benincasa hispida][more]
XP_011658609.19.7e-30089.55phosphoinositide phospholipase C 4 isoform X1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q944C16.1e-19658.28Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 ... [more]
Q8GV432.3e-19559.36Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 ... [more]
Q944C21.1e-18957.74Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 ... [more]
Q390337.0e-18456.68Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 ... [more]
Q9LY516.8e-17956.03Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1DC680.0e+00100.00Phosphoinositide phospholipase C OS=Momordica charantia OX=3673 GN=LOC111018983 ... [more]
A0A1S3C6M63.8e-30289.72Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103497078 PE=4 SV... [more]
A0A0A0LVI74.7e-30089.55Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_1G531180 PE=4... [more]
A0A6J1HKD42.3e-29988.33Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111464902 P... [more]
A0A1S3C4X88.9e-29987.97Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103497078 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT5G58700.14.3e-19758.28phosphatidylinositol-speciwc phospholipase C4 [more]
AT2G40116.11.6e-19659.36Phosphoinositide-specific phospholipase C family protein [more]
AT5G58690.17.9e-19157.74phosphatidylinositol-speciwc phospholipase C5 [more]
AT3G08510.15.0e-18556.68phospholipase C 2 [more]
AT3G08510.25.0e-18556.68phospholipase C 2 [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001192Phosphoinositide phospholipase C familyPRINTSPR00390PHPHLIPASECcoord: 114..132
score: 70.61
coord: 140..160
score: 50.49
coord: 363..384
score: 60.63
coord: 237..254
score: 49.9
coord: 384..402
score: 54.22
coord: 539..549
score: 39.28
IPR001192Phosphoinositide phospholipase C familyPANTHERPTHR10336PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEINcoord: 1..572
IPR000909Phosphatidylinositol-specific phospholipase C, X domainSMARTSM00148plcx_3coord: 109..253
e-value: 1.9E-70
score: 250.0
IPR000909Phosphatidylinositol-specific phospholipase C, X domainPFAMPF00388PI-PLC-Xcoord: 111..253
e-value: 2.7E-50
score: 169.7
IPR000008C2 domainSMARTSM00239C2_3ccoord: 445..553
e-value: 1.7E-13
score: 60.8
IPR000008C2 domainPFAMPF00168C2coord: 446..543
e-value: 1.9E-10
score: 40.9
IPR000008C2 domainPROSITEPS50004C2coord: 419..555
score: 16.017832
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainSMARTSM00149plcy_3coord: 308..425
e-value: 4.0E-60
score: 215.7
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainPFAMPF00387PI-PLC-Ycoord: 337..423
e-value: 8.1E-28
score: 97.1
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainPROSITEPS50008PIPLC_Y_DOMAINcoord: 338..424
score: 35.829601
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 440..573
e-value: 9.9E-27
score: 95.7
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 445..572
NoneNo IPR availableGENE3D1.10.238.10coord: 20..106
e-value: 2.5E-10
score: 42.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 271..296
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 261..300
NoneNo IPR availablePANTHERPTHR10336:SF187PHOSPHOINOSITIDE PHOSPHOLIPASE Ccoord: 1..572
NoneNo IPR availablePROSITEPS50007PIPLC_X_DOMAINcoord: 109..253
score: 43.078499
NoneNo IPR availableCDDcd00275C2_PLC_likecoord: 443..573
e-value: 4.13731E-35
score: 126.502
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilyGENE3D3.20.20.190Phosphatidylinositol (PI) phosphodiesterasecoord: 107..438
e-value: 7.5E-102
score: 342.7
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilySUPERFAMILY51695PLC-like phosphodiesterasescoord: 113..436
IPR015359Phosphoinositide-specific phospholipase C, EF-hand-like domainPFAMPF09279EF-hand_likecoord: 23..98
e-value: 8.4E-5
score: 23.0
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 8..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc03g01710.1Moc03g01710.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016042 lipid catabolic process
biological_process GO:0048015 phosphatidylinositol-mediated signaling
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0006629 lipid metabolic process
biological_process GO:0007165 signal transduction
cellular_component GO:0048046 apoplast
cellular_component GO:0005886 plasma membrane
cellular_component GO:0005773 vacuole
molecular_function GO:0004565 beta-galactosidase activity
molecular_function GO:0004435 phosphatidylinositol phospholipase C activity
molecular_function GO:0008081 phosphoric diester hydrolase activity