Moc02g17520 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g17520
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPectinesterase
Locationchr2: 13106250 .. 13109729 (+)
RNA-Seq ExpressionMoc02g17520
SyntenyMoc02g17520
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACCATTACACTCCAAAATAAGTCCATCTTCCCTCTCTCCAGATCTGCTTCTCTCTTAATTTCCATGGTCTCTCTCCTCGCTCTCTCCCTCTCTCTTTATCTCTCCTCCGCCACCCACCACCGCCGCCTCCAATCTCTCCGCCCTGATATCGCCGCCGCCTGCGCCGCCACCCGTTTCCCGGACCAATGCGGATCCTCCATTTCCCAATCCAACCGCCTCCCGCCAAATCCGACGCCGCTCCAAATCCTCCAAGCCGCCGTCTCGCTCTCGTCGGAGAATCTCCGGACCGCGCAGTCCATGGTCCAGGCAATTCTGGACTCGTCCGCTGACAGCCGGAACCGCAGCGCCGCGGCGCTGGCCTGCCTCGAAGCGCTGGGCTACTCGGAGTATCGAATTTCTCTCGCGAACGACGCCGTTCCACAGGGACGTAACAAGGACGCGCGCGCGTGGCTGAGCGCGTCGTTGCTGTACCAGTCCGGTTGCCGGTCCGGGCTGAAGAACGTGAATGACACGCGCGCGGTGGGTGATACGATGTCGTTTCTTGACTACTTGATTGGGCTGTCGAGCAACGCGCTGAGCATGGCGGTGGCGTACGACGTCTACGGGAACGAGACGGCGGTTTGGCGGCCGCCGAGGACGGAGCGGGACGGGTTCTGGGAGGGTAGTGGGCCCGGCGGGTCGGCGTTCGTCGGCGGCGGGTTTCCGTCGGAGTTGGCGGCGGACGCGACGGTGTGCAAAGATGGGAGCGGCGGGTGTTACAAGACAGTGCAGGAAGCGGTGGATGCGTCGCCGGAGAACGCGGCGGCGCGTAGGTTTGTGATCCACATAAAGGAAGGGGTGTACGAGGAGATCGTGAGAGTGCCATTGAAAAAGAAGAACGTGGTATTTTTTGGAGATGGGATGGGCAAAACCGTCATTTCAGGGTCACTCAATGTGGGCCAGCCTGGGATCTCAACCTACAATTCGGCTACCGTCGGTGAGTTTGTCATTGTTAAAAGCAAAAATTTACGATCGTTTTTTTTTCTTTTTTGGTAGAAAATATTTATGATTGTTATGATCGTTTAGTAGAGTTTGAGAAAAGCAAAATTTATGGTTTTTATGATCGTGGAGTGTATAATACACAAGGTTGTCGGTTTGTGTCAGGATAAAGGGTTTTTTTTAGTGAAAAAATTAATTTTAACCTTAAATCGAGATACAAAGGTATCAACTTTTATCTTCAATTTTCAATTTCAGCCAAACAAATATTGTAATCTTTACTTTAAATTTTTAAAAGCATTACAATTTGATTGATTTTGCAGGAATGAAACTTAATTTTGTTTATATATATAAAAAGTAGATTGATTTTTATTGAGTGGGTAAGTTTTTGGACTTTCTTGATTTTTTTTTATTAATGAGTAGATGAATTAAATTGAATAGGGAGTAAAAACTGGAGTACTTATCTTAATATATGTCTATCTTTTTTTCTCTCTATAATTTTAAATTTACTTCAAAATAGTATACTTAAACATTAAGTTTGATAATAATTTTATATTTAGTTTTTTATTCTTAAAAATATATTTCAACTATTAGCCAAAATTCAAAAACAAAAACTAGTTTTTATTTTTATTTTTTTGGATTGTTTAATAACTATTTCGTTTTTAAAAAATAGGTTTGTTTGGTAACAAATTCAATTTTTTATTTTATGTTTTCAACCTTTTATTTTAAAATTCTATACAGTTTTAAAAATAAAAAAATGAAAATTTATTTTTTAAAAATAGATTTCTGCTTTTGATTTTTTTTCCCTGCTCAACTTTTGTTTTTATACATTATCCCTGACAAAATTCTTTTTTATTGTAAAATTTTTTCTTGTTTAAAATTTTCTCGAGAAAAAATTTGTTTTGATATACTAACTATTATTTCTCTCTCAAAATAAAAAGTTAAAAAGAAAATAGGTTTCAAATGGAGTTTAAATTGTTGCGTTACTTTAAATTTTATGAGCAGTAGTAATAATTTACAGTTTCTAAAAGACTAAAAGAAAAAATAATGGTGAGTTATTTCAGCACTTTTTTTTATTTTTATTTTTTAAATCATTTTCAAGTTCTTAAACTACAGTAGTTATCGATTACTTTGAAGTAATTTTTTGGCTAATCACCCTGTTTCTTAAAAAACACATTTAAAGTAATCCAACTTTTTTTTAATAGCTTTTATACCGAAATAGTAGATTTAAAAGAAATGCTTTTAATTATTTAAAATCACTTAAAATACTATACTAAATATTTTCTAAATTAATAATTGTTTAGGTTGACTTTTTAAAAAAAATTGAAAAGTTATTCCAAATAACCTATAAATGACTTGCACCAAATGAATTAAAGTCAGGGTGGTTAGGTCGAGAAATACCAACTATTTTTTTTGGTTCAAAATATAAATTTAATTTTAAAAATGTTTATTTAAAAAATATGAACAAAATAGTACATAATTAACGCCCTATTTGTTCATTATTTTTATTTTTAAAATCATGCTTGTTTCTTCACAATTTATTTGGTCACGTTTTTTAAATAATACATTTGATTAAACGTAAAAACTTTTTTGAAATCTATTATTTTAAAAATTACAGAAAAAAAAGTAATACATTAATGTTTACAAACTTAATTTTTAAAAACAAAAATGTTATCCAACCAGAAGTAGGCCACAATTATTATATTTCTTTTTTTTTTAATTTTCAAATGTTTCTTTTTAATTTTTAATTTTTTTTTTGAAAATTAGGTTTATAAACAATACTTTTCGTATGAAACAGACTTAAAAATTTGATAGGAAATTATTGCAGGAGTTGCTGGTGACGGATTCATGGCCAGTGGGCTGACCATTGAGAACACAGCAGGGCCTGATGCCCATCAAGCTGTGGCCTTCAGATCAGACAGCGATCTCTCCGTTATCCAAAACTGTGAGTTTATAGGCAACCAAGACACTCTCTACGCCCATTCCCTACGCCAATTCTACAACTCCTGCCGAATCCTAGGCAATGTGGACTTCATCTTTGGAAACTCGGCCTCCATCTTCCAGGACTGCCACATCCTACTCCGCCCTCGGCCCACCGGTGGCGACTACCCCGTCACTGCCCACGGTAGGACCGACCCCGCCCAGTCCACCGGCTTCGTCTTCCTCAATTGCCTTGTTAACGGCACCGAGGAGTTCATGTCCTTGTACTATGCCGATCCTCGGGTGCATAGGACCTTCTTGGGGCGGCCCTGGAAGGAGTATTCGAGGACGGTTTTCATACATTGTGATCTCGAAGCTGTTGTTTCCCCTTTTGGGTGGTCACCGTGGAGCGGGGAGTTTGCTTTGAACACTCTGTTTTATGGGGAGTTTGAGAATTTCGGGATCGGTTCGAATTTGTCCGAGAGAGTCGGGTGGAGTAGCCAGATCCCAGCGGAGTATGTTTCTACTTACTCGGTTGAGAACTTCATTCAAGGAAATGAATGGATTCCATTAAGCTCGTGA

mRNA sequence

ATGACCATTACACTCCAAAATAAGTCCATCTTCCCTCTCTCCAGATCTGCTTCTCTCTTAATTTCCATGGTCTCTCTCCTCGCTCTCTCCCTCTCTCTTTATCTCTCCTCCGCCACCCACCACCGCCGCCTCCAATCTCTCCGCCCTGATATCGCCGCCGCCTGCGCCGCCACCCGTTTCCCGGACCAATGCGGATCCTCCATTTCCCAATCCAACCGCCTCCCGCCAAATCCGACGCCGCTCCAAATCCTCCAAGCCGCCGTCTCGCTCTCGTCGGAGAATCTCCGGACCGCGCAGTCCATGGTCCAGGCAATTCTGGACTCGTCCGCTGACAGCCGGAACCGCAGCGCCGCGGCGCTGGCCTGCCTCGAAGCGCTGGGCTACTCGGAGTATCGAATTTCTCTCGCGAACGACGCCGTTCCACAGGGACGTAACAAGGACGCGCGCGCGTGGCTGAGCGCGTCGTTGCTGTACCAGTCCGGTTGCCGGTCCGGGCTGAAGAACGTGAATGACACGCGCGCGGTGGGTGATACGATGTCGTTTCTTGACTACTTGATTGGGCTGTCGAGCAACGCGCTGAGCATGGCGGTGGCGTACGACGTCTACGGGAACGAGACGGCGGTTTGGCGGCCGCCGAGGACGGAGCGGGACGGGTTCTGGGAGGGTAGTGGGCCCGGCGGGTCGGCGTTCGTCGGCGGCGGGTTTCCGTCGGAGTTGGCGGCGGACGCGACGGTGTGCAAAGATGGGAGCGGCGGGTGTTACAAGACAGTGCAGGAAGCGGTGGATGCGTCGCCGGAGAACGCGGCGGCGCGTAGGTTTGTGATCCACATAAAGGAAGGGGTGTACGAGGAGATCGTGAGAGTGCCATTGAAAAAGAAGAACGTGGTATTTTTTGGAGATGGGATGGGCAAAACCGTCATTTCAGGGTCACTCAATGTGGGCCAGCCTGGGATCTCAACCTACAATTCGGCTACCGTCGGAGTTGCTGGTGACGGATTCATGGCCAGTGGGCTGACCATTGAGAACACAGCAGGGCCTGATGCCCATCAAGCTGTGGCCTTCAGATCAGACAGCGATCTCTCCGTTATCCAAAACTGTGAGTTTATAGGCAACCAAGACACTCTCTACGCCCATTCCCTACGCCAATTCTACAACTCCTGCCGAATCCTAGGCAATGTGGACTTCATCTTTGGAAACTCGGCCTCCATCTTCCAGGACTGCCACATCCTACTCCGCCCTCGGCCCACCGGTGGCGACTACCCCGTCACTGCCCACGGTAGGACCGACCCCGCCCAGTCCACCGGCTTCGTCTTCCTCAATTGCCTTGTTAACGGCACCGAGGAGTTCATGTCCTTGTACTATGCCGATCCTCGGGTGCATAGGACCTTCTTGGGGCGGCCCTGGAAGGAGTATTCGAGGACGGTTTTCATACATTGTGATCTCGAAGCTGTTGTTTCCCCTTTTGGGTGGTCACCGTGGAGCGGGGAGTTTGCTTTGAACACTCTGTTTTATGGGGAGTTTGAGAATTTCGGGATCGGTTCGAATTTGTCCGAGAGAGTCGGGTGGAGTAGCCAGATCCCAGCGGAGTATGTTTCTACTTACTCGGTTGAGAACTTCATTCAAGGAAATGAATGGATTCCATTAAGCTCGTGA

Coding sequence (CDS)

ATGACCATTACACTCCAAAATAAGTCCATCTTCCCTCTCTCCAGATCTGCTTCTCTCTTAATTTCCATGGTCTCTCTCCTCGCTCTCTCCCTCTCTCTTTATCTCTCCTCCGCCACCCACCACCGCCGCCTCCAATCTCTCCGCCCTGATATCGCCGCCGCCTGCGCCGCCACCCGTTTCCCGGACCAATGCGGATCCTCCATTTCCCAATCCAACCGCCTCCCGCCAAATCCGACGCCGCTCCAAATCCTCCAAGCCGCCGTCTCGCTCTCGTCGGAGAATCTCCGGACCGCGCAGTCCATGGTCCAGGCAATTCTGGACTCGTCCGCTGACAGCCGGAACCGCAGCGCCGCGGCGCTGGCCTGCCTCGAAGCGCTGGGCTACTCGGAGTATCGAATTTCTCTCGCGAACGACGCCGTTCCACAGGGACGTAACAAGGACGCGCGCGCGTGGCTGAGCGCGTCGTTGCTGTACCAGTCCGGTTGCCGGTCCGGGCTGAAGAACGTGAATGACACGCGCGCGGTGGGTGATACGATGTCGTTTCTTGACTACTTGATTGGGCTGTCGAGCAACGCGCTGAGCATGGCGGTGGCGTACGACGTCTACGGGAACGAGACGGCGGTTTGGCGGCCGCCGAGGACGGAGCGGGACGGGTTCTGGGAGGGTAGTGGGCCCGGCGGGTCGGCGTTCGTCGGCGGCGGGTTTCCGTCGGAGTTGGCGGCGGACGCGACGGTGTGCAAAGATGGGAGCGGCGGGTGTTACAAGACAGTGCAGGAAGCGGTGGATGCGTCGCCGGAGAACGCGGCGGCGCGTAGGTTTGTGATCCACATAAAGGAAGGGGTGTACGAGGAGATCGTGAGAGTGCCATTGAAAAAGAAGAACGTGGTATTTTTTGGAGATGGGATGGGCAAAACCGTCATTTCAGGGTCACTCAATGTGGGCCAGCCTGGGATCTCAACCTACAATTCGGCTACCGTCGGAGTTGCTGGTGACGGATTCATGGCCAGTGGGCTGACCATTGAGAACACAGCAGGGCCTGATGCCCATCAAGCTGTGGCCTTCAGATCAGACAGCGATCTCTCCGTTATCCAAAACTGTGAGTTTATAGGCAACCAAGACACTCTCTACGCCCATTCCCTACGCCAATTCTACAACTCCTGCCGAATCCTAGGCAATGTGGACTTCATCTTTGGAAACTCGGCCTCCATCTTCCAGGACTGCCACATCCTACTCCGCCCTCGGCCCACCGGTGGCGACTACCCCGTCACTGCCCACGGTAGGACCGACCCCGCCCAGTCCACCGGCTTCGTCTTCCTCAATTGCCTTGTTAACGGCACCGAGGAGTTCATGTCCTTGTACTATGCCGATCCTCGGGTGCATAGGACCTTCTTGGGGCGGCCCTGGAAGGAGTATTCGAGGACGGTTTTCATACATTGTGATCTCGAAGCTGTTGTTTCCCCTTTTGGGTGGTCACCGTGGAGCGGGGAGTTTGCTTTGAACACTCTGTTTTATGGGGAGTTTGAGAATTTCGGGATCGGTTCGAATTTGTCCGAGAGAGTCGGGTGGAGTAGCCAGATCCCAGCGGAGTATGTTTCTACTTACTCGGTTGAGAACTTCATTCAAGGAAATGAATGGATTCCATTAAGCTCGTGA

Protein sequence

MTITLQNKSIFPLSRSASLLISMVSLLALSLSLYLSSATHHRRLQSLRPDIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGSAFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPRPTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQIPAEYVSTYSVENFIQGNEWIPLSS
Homology
BLAST of Moc02g17520 vs. NCBI nr
Match: XP_038900555.1 (probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida])

HSP 1 Score: 823.9 bits (2127), Expect = 8.0e-235
Identity = 424/548 (77.37%), Postives = 467/548 (85.22%), Query Frame = 0

Query: 9   SIFPLSRSASLLISMVSLLAL-SLSLYLSSATHHRRLQSLRPDIAAACAATRFPDQCGSS 68
           SIFP   S SL ISM SLL L  L L+ SSA H    Q++ P IAAAC ATRFPDQC SS
Sbjct: 64  SIFPNQTSVSLFISMPSLLFLFFLPLHFSSAAH----QTILPGIAAACGATRFPDQCASS 123

Query: 69  ISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADS--RNRSAAALACLEA 128
           ISQS+RLP NPTPLQI+QAAV LSSENL+ A+SMV+AILDSS  S  RNR+AAAL+CLE 
Sbjct: 124 ISQSSRLPQNPTPLQIIQAAVWLSSENLKIAKSMVKAILDSSEKSPNRNRTAAALSCLEG 183

Query: 129 LGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTMSFLDYL 188
           LGYSEYRISL+NDA+  GRNKDARAWLSASLLYQ+GCRSGLK VNDTR V +TMSFLD L
Sbjct: 184 LGYSEYRISLSNDAIAIGRNKDARAWLSASLLYQTGCRSGLKYVNDTREVDETMSFLDNL 243

Query: 189 IGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGSAFVGGGFPSELAADATV 248
           I LSSNALSMAVAYD++GNET  W PPRTERDGFWE SG       G G P++  A+ATV
Sbjct: 244 IVLSSNALSMAVAYDIFGNETEAWGPPRTERDGFWERSGGTAVVGTGLGIPAKTEANATV 303

Query: 249 CKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGMGKT 308
           CK+GS GCY TVQ AV+A+PEN+A  RFVIHIKEGVYEE VRV L+KKN+VF GDGMGKT
Sbjct: 304 CKEGSEGCYTTVQAAVEAAPENSAV-RFVIHIKEGVYEESVRVGLEKKNLVFLGDGMGKT 363

Query: 309 VISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSVIQN 368
           VI+GSLNVGQPGISTY SATVGV GDGFMAS LTI+NTAGPDAHQAVAFRSDSDLS+I N
Sbjct: 364 VITGSLNVGQPGISTYKSATVGVLGDGFMASRLTIQNTAGPDAHQAVAFRSDSDLSIIHN 423

Query: 369 CEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPR----PTGGDYP 428
           CEFIGNQDTLYAHSLRQFYNSCRI+GNVDFIFGNSASIFQ+CHIL+RPR      G D P
Sbjct: 424 CEFIGNQDTLYAHSLRQFYNSCRIVGNVDFIFGNSASIFQNCHILVRPRQLNPQLGDDNP 483

Query: 429 VTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIHCDL 488
           +TAHGRTDPAQSTGFVF NCLVNGTEEFMSLYYA+P+ H+TFLGRPWKEYSRT+FIHCDL
Sbjct: 484 ITAHGRTDPAQSTGFVFQNCLVNGTEEFMSLYYANPQAHKTFLGRPWKEYSRTIFIHCDL 543

Query: 489 EAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQIPAEYVSTYSVENFIQ 548
           EA+VS  GW PWSG+F L TL+YGEFENF IGSN S+RVGWS+QIPAEYV +YSV+NFIQ
Sbjct: 544 EALVSSSGWMPWSGDFGLKTLYYGEFENFEIGSNSSKRVGWSNQIPAEYVFSYSVQNFIQ 603

Query: 549 GNEWIPLS 550
           GNEWIP S
Sbjct: 604 GNEWIPSS 606

BLAST of Moc02g17520 vs. NCBI nr
Match: XP_038902126.1 (probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida])

HSP 1 Score: 799.7 bits (2064), Expect = 1.6e-227
Identity = 411/560 (73.39%), Postives = 462/560 (82.50%), Query Frame = 0

Query: 8   KSIFPLSRSASLLISMVSLLALS-LSLY-----LSSAT----HHRRLQSLRP----DIAA 67
           K+ FP+S+S  L +SM SL  LS LSL+     LSSA     HH       P     I  
Sbjct: 5   KTHFPISKSTLLFLSMASLSFLSFLSLFSFFLSLSSAARHRHHHLSTHFTNPPSIDGILH 64

Query: 68  ACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSR 127
           AC ATRFPDQCGSS+S S+ LPPNP+PLQILQ+AVSLSS+ L TAQSMV+ ILDSSA + 
Sbjct: 65  ACKATRFPDQCGSSLSHSS-LPPNPSPLQILQSAVSLSSDGLSTAQSMVKQILDSSAKNL 124

Query: 128 NRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTR 187
           NR+ AA  CLE L  S+YRISL NDA+ +GR KDARAWLSASLLY   C S LK  NDT+
Sbjct: 125 NRTTAAKNCLEVLRNSDYRISLTNDALRRGRTKDARAWLSASLLYHYDCWSALKYANDTQ 184

Query: 188 AVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGSAFVGG 247
            V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G G      G
Sbjct: 185 LVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLGFSG 244

Query: 248 GFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKK 307
           GFPS++AADATVCKDG+GGCY TVQEAVDA+PENAAARRFVIHIKEGVYEEIVRVPL+KK
Sbjct: 245 GFPSKMAADATVCKDGAGGCYGTVQEAVDAAPENAAARRFVIHIKEGVYEEIVRVPLEKK 304

Query: 308 NVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVA 367
           NVVF GDGMGKTVI+GSLNVGQPG+STYN+ATVGV GDGFMASGLTI+NTAGPDAHQAVA
Sbjct: 305 NVVFLGDGMGKTVITGSLNVGQPGLSTYNTATVGVLGDGFMASGLTIQNTAGPDAHQAVA 364

Query: 368 FRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRP 427
           FRSDSDLSVIQ+CEFIGNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC IL+RP
Sbjct: 365 FRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEILVRP 424

Query: 428 R----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWK 487
           R      G +  +TA GRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ FLGRPWK
Sbjct: 425 RQLKPEKGENNAITAQGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGRPWK 484

Query: 488 EYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQIPAE 547
           EYSRTVFIHC+LE++V+P GW PWSG+FAL TLFYGEFEN G+GS  SERVGWSSQIPA+
Sbjct: 485 EYSRTVFIHCNLESLVTPAGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQIPAQ 544

Query: 548 YVSTYSVENFIQGNEWIPLS 550
           +V +YSV+NFIQG+EWIP S
Sbjct: 545 HVFSYSVQNFIQGDEWIPSS 563

BLAST of Moc02g17520 vs. NCBI nr
Match: XP_008460902.1 (PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor 51 [Cucumis melo])

HSP 1 Score: 793.9 bits (2049), Expect = 8.9e-226
Identity = 400/566 (70.67%), Postives = 456/566 (80.57%), Query Frame = 0

Query: 8   KSIFPLSRSASLLISMVSL------------LALSLSLYLSSATHHRRLQSL-------- 67
           K  FP+S+S  L +SM SL             +LSLS   S+  HH     L        
Sbjct: 6   KPTFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTP 65

Query: 68  RPDIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILD 127
              I  AC +TRFPDQCGSS+S S+  PPNP+PLQILQ+A++LSS+ L TAQS+V+ ILD
Sbjct: 66  SDQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLVKQILD 125

Query: 128 SSADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLK 187
           SSA + NR+ AA  CLE L  S+YRISL NDA+ +GR KDARAWLSASLLY   C S LK
Sbjct: 126 SSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALK 185

Query: 188 NVNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGG 247
             NDT+ V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G G 
Sbjct: 186 YANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGS 245

Query: 248 SAFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVR 307
                GGFP+++ ADATVCKDGSGGCY ++Q+AVDA+PENAAARRFVIHIKEGVYEEIVR
Sbjct: 246 GLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVR 305

Query: 308 VPLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPD 367
           VPL+KKNVVF GDGMGKTVI+GSLN GQPGISTYN+AT+GV GDGFMASGLTI+NTAGPD
Sbjct: 306 VPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPD 365

Query: 368 AHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDC 427
           AHQAVAFRSDSDLSVIQ+CEFIGNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC
Sbjct: 366 AHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDC 425

Query: 428 HILLRPR----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTF 487
            IL+RPR      G +  +TAHGRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ F
Sbjct: 426 EILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNF 485

Query: 488 LGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWS 547
           LGRPWKEYSRTVFIHC+LEA+VSP GW PWSG+FAL TLFYGEFEN G+GS  SERVGWS
Sbjct: 486 LGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWS 545

Query: 548 SQIPAEYVSTYSVENFIQGNEWIPLS 550
           SQIPA++V +YSV+NFIQG+EWIP S
Sbjct: 546 SQIPAQHVFSYSVQNFIQGDEWIPSS 571

BLAST of Moc02g17520 vs. NCBI nr
Match: XP_004147375.2 (probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146.1 hypothetical protein Csa_005971 [Cucumis sativus])

HSP 1 Score: 792.7 bits (2046), Expect = 2.0e-225
Identity = 406/565 (71.86%), Postives = 457/565 (80.88%), Query Frame = 0

Query: 8   KSIFPLSRSASLLISMVSLLAL----------SLSLYLSSATHHRRLQSLRP-------- 67
           K  FP S+S  L +SM SL  L          SLSL  SSA HH       P        
Sbjct: 6   KPTFPFSKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLPSHFTNYPS 65

Query: 68  -DIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDS 127
             I  AC +TRFPDQCGSS+S S+ LPPNP+PLQILQ+A++LSS+ L TAQS+V+ ILDS
Sbjct: 66  DQILHACKSTRFPDQCGSSLSHSS-LPPNPSPLQILQSAIALSSDGLTTAQSLVKQILDS 125

Query: 128 SADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKN 187
           SA + NR+ AA  CLE L  S+YRISL NDA+  GR KDARAWLSASLLY   C S LK 
Sbjct: 126 SATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWSALKY 185

Query: 188 VNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGS 247
            NDT+ V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G  G 
Sbjct: 186 ANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGSGL 245

Query: 248 AFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRV 307
            F  GGFP+++ ADATVCKDGSGGCY ++Q+AVDA+PENAAARRFVIHIKEGVYEEIVRV
Sbjct: 246 GF-SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRV 305

Query: 308 PLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDA 367
           PL+KKNVVF GDGMGKTVI+GSLNVGQPG+STYN+AT+GV GDGFMASGLTI+NTAGPDA
Sbjct: 306 PLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTAGPDA 365

Query: 368 HQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCH 427
           HQAVAFRSDSDLSVIQ+CEF+GNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC 
Sbjct: 366 HQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 425

Query: 428 ILLRPR----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFL 487
           IL+RPR      G +  VTAHGRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ FL
Sbjct: 426 ILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVHKNFL 485

Query: 488 GRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSS 547
           GRPWKEYSRTVFIHC+LEA+VSP GW PWSG+FAL TLFYGEFEN G GS  SERVGWSS
Sbjct: 486 GRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSS 545

Query: 548 QIPAEYVSTYSVENFIQGNEWIPLS 550
           QIPA++V +YSVENFIQG+EWIP S
Sbjct: 546 QIPAQHVFSYSVENFIQGDEWIPSS 568

BLAST of Moc02g17520 vs. NCBI nr
Match: KAA0040770.1 (putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa] >TYK02086.1 putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa])

HSP 1 Score: 784.3 bits (2024), Expect = 7.1e-223
Identity = 383/503 (76.14%), Postives = 434/503 (86.28%), Query Frame = 0

Query: 51  IAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSA 110
           I  AC +TRFPDQCGSS+S S+ LPPNP+PLQILQ+A++LSS+ L TAQS+V+ ILDSSA
Sbjct: 42  ILHACKSTRFPDQCGSSLSHSS-LPPNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSA 101

Query: 111 DSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVN 170
            + NR+ AA  CLE L  S+YRISL NDA+ +GR KDARAWLSASLLY   C S LK  N
Sbjct: 102 TNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYAN 161

Query: 171 DTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGSAF 230
           DT+ V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G G    
Sbjct: 162 DTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLG 221

Query: 231 VGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPL 290
             GGFP+++ ADATVCKDGSGGCY ++Q+AVDA+PENAAARRFVIHIKEGVYEEIVRVPL
Sbjct: 222 FSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPL 281

Query: 291 KKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQ 350
           +KKNVVF GDGMGKTVI+GSLN GQPGISTYN+AT+GV GDGFMASGLTI+NTAGPDAHQ
Sbjct: 282 EKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQ 341

Query: 351 AVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHIL 410
           AVAFRSDSDLSVIQ+CEFIGNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC IL
Sbjct: 342 AVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL 401

Query: 411 LRPR----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGR 470
           +RPR      G +  +TAHGRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ FLGR
Sbjct: 402 IRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGR 461

Query: 471 PWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQI 530
           PWKEYSRTVFIHC+LEA+VSP GW PWSG+FAL TLFYGEFEN G+GS  SERVGWSSQI
Sbjct: 462 PWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQI 521

Query: 531 PAEYVSTYSVENFIQGNEWIPLS 550
           PA++V +YSV+NFIQG+EWIP S
Sbjct: 522 PAQHVFSYSVQNFIQGDEWIPSS 543

BLAST of Moc02g17520 vs. ExPASy Swiss-Prot
Match: Q9LXD9 (Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3702 GN=PME51 PE=2 SV=1)

HSP 1 Score: 656.0 bits (1691), Expect = 3.8e-187
Identity = 336/552 (60.87%), Postives = 411/552 (74.46%), Query Frame = 0

Query: 23  MVSLLALSLSLYL-----SSATHHRR-----------LQSLRPDIAAACAATRFPDQCGS 82
           M S+L L  SL+L     SS+ HH               SL   I  AC ATR+PDQC S
Sbjct: 1   MSSILILLFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVS 60

Query: 83  SISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEAL 142
           S+S+  R+PP+P P+QI+ +A+S S +NL+TAQS +++I+DSS  + NR+ AA  CL+ L
Sbjct: 61  SLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLL 120

Query: 143 GYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTMSFLDYLI 202
            YSE+R    + A+ +G+ KDARAW+SA+L+YQ    S LK VNDT  VG+TMSFLD LI
Sbjct: 121 TYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLI 180

Query: 203 GLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPG----GSAFVGGGFPSELAAD 262
            ++SNALSM V+YD +G+  A W  P TERDGFWE +GPG     S  +  GFPS L  D
Sbjct: 181 HVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKED 240

Query: 263 ATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGM 322
            TVCKDG  G YKTVQ+AV+A+PE+   R+FVI I EGVYEE V VP +KKNVVF GDGM
Sbjct: 241 VTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGM 300

Query: 323 GKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSV 382
           GKTVI+GSLN G PGI+TYN+ATVGV GDGFMA  LT +NTAGPDAHQAVAFRSDSD S+
Sbjct: 301 GKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSL 360

Query: 383 IQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPR----PTGG 442
           I+NCEF+GNQDTLYAH LRQFY +CRI GNVDFIFGNSA++FQDC IL+ PR      G 
Sbjct: 361 IENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGE 420

Query: 443 DYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIH 502
              VTA GR DP+QSTGFVFLNCL+NGTEE+M L+ A+P+VH+ FLGRPWK+YSRTVFI 
Sbjct: 421 KNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIG 480

Query: 503 CDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQIPAEYVSTYSVEN 551
           C+LEA+++P GW PWSG+FAL TL+YGE +N G GS+ S+RV WSSQIP E+V  YSV N
Sbjct: 481 CNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVAN 540

BLAST of Moc02g17520 vs. ExPASy Swiss-Prot
Match: Q8L7Q7 (Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3702 GN=PME64 PE=2 SV=2)

HSP 1 Score: 627.1 bits (1616), Expect = 1.9e-178
Identity = 324/567 (57.14%), Postives = 408/567 (71.96%), Query Frame = 0

Query: 13  LSRSASLLI---SMVSLLALSLSLYL----SSATHHR--------------RLQSLRPDI 72
           LSR+  L+I   S + LL +SL +Y     SS  HH                     P I
Sbjct: 31  LSRNGILIIIAASCILLLLISLLIYATVSKSSRNHHNPSHQTPTSDDHPPPETPPSPPPI 90

Query: 73  A---AACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDS 132
           A    AC ATRFPD C +S+S+  ++PP+P P+QI+ +A+S+S ENL++ QS +Q+ILDS
Sbjct: 91  AQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDS 150

Query: 133 SADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKN 192
           SA +RNR+  A  CLE L YS++R    + AV  G  KDARAW+SA+L YQ  C SGLK 
Sbjct: 151 SAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKT 210

Query: 193 VNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGS 252
           VNDT+ V DT++F + L+ L+ NALSM +++D +G++   W  P TERDGFWE +GP   
Sbjct: 211 VNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLG 270

Query: 253 AFVGG----GFPSELAADATVCKDGSGGC-YKTVQEAVDASPENAAARRFVIHIKEGVYE 312
           +  G     GFPS L  D TVCK+G   C YKTVQEAVD++P+     +FVI I+EGVYE
Sbjct: 271 SGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYE 330

Query: 313 EIVRVPLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENT 372
           E VRVP +KKNVVF GDGMGKTVI+GSLNVGQPG++T+ SATVGV GDGFMA  LTIENT
Sbjct: 331 ETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENT 390

Query: 373 AGPDAHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASI 432
           AG DAHQAVAFRSDSD SV++NCEF+GNQDTLYAHSLRQFY  CRI GNVDFIFGNSA++
Sbjct: 391 AGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAV 450

Query: 433 FQDCHILLRPRPT-----GGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPR 492
           FQDC IL+  + +     G +  +TAHGR D +QSTGFVFLNC +NGTEE+M  + A+P 
Sbjct: 451 FQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPE 510

Query: 493 VHRTFLGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSE 546
            H+ FLGRPWKE+SRTVF++C+LE+++SP GW PW+G+FAL TL+YGE++N G GS  S 
Sbjct: 511 GHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSS 570

BLAST of Moc02g17520 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 320.9 bits (821), Expect = 2.9e-86
Identity = 202/531 (38.04%), Postives = 290/531 (54.61%), Query Frame = 0

Query: 23  MVSLLALSLSLYLSSATHHRRLQSLRPDIAAACAATRFPDQCGSSISQSNRLPPNPTPLQ 82
           M   + +++SL L S +H     S   ++ + C  T  P  C   ++Q   +        
Sbjct: 1   MALRILITVSLVLFSLSHTSFGYS-PEEVKSWCGKTPNPQPCEYFLTQKTDVTSIKQDTD 60

Query: 83  ILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEALGYSEYRISLANDAVPQ 142
             + ++ L+ E   TAQS     L S   +    AA   C E    +  +++  +++ P 
Sbjct: 61  FYKISLQLALERATTAQSRTYT-LGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPG 120

Query: 143 GRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVY 202
               D + WLS +L     CR+ L++          +   +Y++ L SN ++  ++  + 
Sbjct: 121 CTKVDKQTWLSTALTNLETCRASLED----------LGVPEYVLPLLSNNVTKLISNTLS 180

Query: 203 GNETAVWRPPRTERDGFWEGSGPGGSAFVGGGFPSELAADATVCKDGSGGCYKTVQEAVD 262
            N+     P  + +DGF     PG    +     +   A+  V +DGSG   KT+QEAV 
Sbjct: 181 LNKVPYNEP--SYKDGFPTWVKPGDRKLL----QTTPRANIVVAQDGSGN-VKTIQEAV- 240

Query: 263 ASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYN 322
           A+   A   R+VI+IK G Y E + V  K KN++F GDG+GKT+I+GS +VG  G +T+ 
Sbjct: 241 AAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVG-GGATTFK 300

Query: 323 SATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQ 382
           SATV V GD F+A  +TI NTAGP+ HQAVA RS SDLSV   C F G QDTLY HS RQ
Sbjct: 301 SATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQ 360

Query: 383 FYNSCRILGNVDFIFGNSASIFQDCHILLRPRPTGGDYPVTAHGRTDPAQSTGFVFLNCL 442
           FY  C I G VDFIFGN+A + Q+C+I  R +P      +TA GRTDP QSTG +  NC 
Sbjct: 361 FYRECDIYGTVDFIFGNAAVVLQNCNIFAR-KPPNRTNTLTAQGRTDPNQSTGIIIHNCR 420

Query: 443 VNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTL 502
           V    +   +  +     +TFLGRPWK+YSRTV+I   L+++++P GW  WSG+FALNTL
Sbjct: 421 VTAASDLKPVQSS----VKTFLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTL 480

Query: 503 FYGEFENFGIGSNLSERVGWSSQ---IPAEYVSTYSVENFIQGNEWIPLSS 551
           +Y E+ N G GS+ + RV W           VS ++V NFI GN W+P ++
Sbjct: 481 YYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPATN 503

BLAST of Moc02g17520 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 312.4 bits (799), Expect = 1.0e-83
Identity = 191/504 (37.90%), Postives = 279/504 (55.36%), Query Frame = 0

Query: 50  DIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSS 109
           D+ A C+ T  P  C   ++ ++   P  +  + L+ ++ L  +    A++    +    
Sbjct: 29  DVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKC 88

Query: 110 ADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNV 169
            D+R + AA   C++    +  +I+   D   +    DA+ WLS +L     CR+G   +
Sbjct: 89  RDTREK-AAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLEL 148

Query: 170 NDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAV-WRPPRTERDGFWEGSGPGGS 229
             T          D ++ L SN +S  +   +  N+    + PP  E+DGF     PG  
Sbjct: 149 GVT----------DIVLPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDR 208

Query: 230 AFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRV 289
             +    P +   +A V KDGSG  +KT++EA+DA+   + + RFVI++K+GVY E   +
Sbjct: 209 KLLQSSTPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NL 268

Query: 290 PLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDA 349
            ++KKNV+  GDG+GKT+I+GS +VG  G +T+NSATV   GDGF+A G+T  NTAG   
Sbjct: 269 EIRKKNVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITFRNTAGASN 328

Query: 350 HQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCH 409
            QAVA RS SDLSV   C F   QDTLY HS RQFY  C + G VDFIFGN+A++ Q+C+
Sbjct: 329 EQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCN 388

Query: 410 ILLRPRPTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPW 469
           I  R RP      +TA GR+DP Q+TG +  N  V    +   +  +     +T+LGRPW
Sbjct: 389 IFAR-RPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRPW 448

Query: 470 KEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSS---Q 529
           ++YSRTVF+   L++++ P GW  W G FAL TLFY EF+N G G++ S RV W      
Sbjct: 449 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 504

Query: 530 IPAEYVSTYSVENFIQGNEWIPLS 550
             A   S ++V  F+ G  WIP S
Sbjct: 509 GSASEASKFTVGTFLAGGSWIPSS 504

BLAST of Moc02g17520 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 6.3e-81
Identity = 191/547 (34.92%), Postives = 290/547 (53.02%), Query Frame = 0

Query: 13  LSRSASLLISMVSLLALSLSLYLSSATHHRRLQSLRPDIAAACAATRFPDQCGSSISQSN 72
           +S S+ LLISMV  + + +S+  S       + +    I   CA T + + C  ++ +  
Sbjct: 19  ISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRKDA 78

Query: 73  RLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEALGYSEYR 132
           +      PL++++ A + + + +       Q +++   D R + A    C E + Y+   
Sbjct: 79  K--DTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALD-QCKELMDYAIGE 138

Query: 133 ISLANDAVPQGRNKDA-------RAWLSASLLYQSGCRSGLKNVNDTRAVGDTM-SFLDY 192
           +S + + + +             R WLSA++ ++  C  G +        G+T+   L  
Sbjct: 139 LSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGN--AGETIKKALKT 198

Query: 193 LIGLSSNALSMAVAYDVYGNETAV--WRPPRTERDGFWEGSGPGGSAFVGGGFPSELAAD 252
            + L+ N L+M      Y  +  +      R     F           +     SE+  D
Sbjct: 199 AVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNAPM-SEVKPD 258

Query: 253 ATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGM 312
             V +DGSG  YKT+ EA++  P+      FV+HIKEG+Y+E V+V     ++VF GDG 
Sbjct: 259 IVVAQDGSGQ-YKTINEALNFVPKKKNT-TFVVHIKEGIYKEYVQVNRSMTHLVFIGDGP 318

Query: 313 GKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSV 372
            KTVISGS +  + GI+TY +ATV + GD F+A  +  ENTAG   HQAVA R  +D S+
Sbjct: 319 DKTVISGSKSY-KDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESI 378

Query: 373 IQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPRPTGGDYPV 432
             NC+F G QDTLYAHS RQFY  C I G +DF+FG++A++FQ+C +L+R        P+
Sbjct: 379 FYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPI 438

Query: 433 TAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIHCDLE 492
           TAHGR DP +STGFV   C + G  +    Y A     +T+LGRPWKEYSRT+ ++  + 
Sbjct: 439 TAHGRKDPRESTGFVLQGCTIVGEPD----YLAVKEQSKTYLGRPWKEYSRTIIMNTFIP 498

Query: 493 AVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWS--SQIPAEYVSTYSVENFI 548
             V P GW PW GEF LNTLFY E +N G G+ +++RV W    ++  E +  ++   +I
Sbjct: 499 DFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYI 552

BLAST of Moc02g17520 vs. ExPASy TrEMBL
Match: A0A1S3CD05 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 4.3e-226
Identity = 400/566 (70.67%), Postives = 456/566 (80.57%), Query Frame = 0

Query: 8   KSIFPLSRSASLLISMVSL------------LALSLSLYLSSATHHRRLQSL-------- 67
           K  FP+S+S  L +SM SL             +LSLS   S+  HH     L        
Sbjct: 6   KPTFPISKSTLLFLSMASLSFLSFLSLSFFFFSLSLSSSSSAVRHHHSHSHLPSHFTNTP 65

Query: 68  RPDIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILD 127
              I  AC +TRFPDQCGSS+S S+  PPNP+PLQILQ+A++LSS+ L TAQS+V+ ILD
Sbjct: 66  SDQILHACKSTRFPDQCGSSLSHSSXSPPNPSPLQILQSAIALSSDGLTTAQSLVKQILD 125

Query: 128 SSADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLK 187
           SSA + NR+ AA  CLE L  S+YRISL NDA+ +GR KDARAWLSASLLY   C S LK
Sbjct: 126 SSATNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALK 185

Query: 188 NVNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGG 247
             NDT+ V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G G 
Sbjct: 186 YANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGS 245

Query: 248 SAFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVR 307
                GGFP+++ ADATVCKDGSGGCY ++Q+AVDA+PENAAARRFVIHIKEGVYEEIVR
Sbjct: 246 GLGFSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVR 305

Query: 308 VPLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPD 367
           VPL+KKNVVF GDGMGKTVI+GSLN GQPGISTYN+AT+GV GDGFMASGLTI+NTAGPD
Sbjct: 306 VPLEKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPD 365

Query: 368 AHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDC 427
           AHQAVAFRSDSDLSVIQ+CEFIGNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC
Sbjct: 366 AHQAVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDC 425

Query: 428 HILLRPR----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTF 487
            IL+RPR      G +  +TAHGRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ F
Sbjct: 426 EILIRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNF 485

Query: 488 LGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWS 547
           LGRPWKEYSRTVFIHC+LEA+VSP GW PWSG+FAL TLFYGEFEN G+GS  SERVGWS
Sbjct: 486 LGRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWS 545

Query: 548 SQIPAEYVSTYSVENFIQGNEWIPLS 550
           SQIPA++V +YSV+NFIQG+EWIP S
Sbjct: 546 SQIPAQHVFSYSVQNFIQGDEWIPSS 571

BLAST of Moc02g17520 vs. ExPASy TrEMBL
Match: A0A0A0LQ80 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 9.6e-226
Identity = 406/565 (71.86%), Postives = 457/565 (80.88%), Query Frame = 0

Query: 8   KSIFPLSRSASLLISMVSLLAL----------SLSLYLSSATHHRRLQSLRP-------- 67
           K  FP S+S  L +SM SL  L          SLSL  SSA HH       P        
Sbjct: 6   KPTFPFSKSTLLFLSMASLSFLSFLSLFFFFSSLSLSSSSADHHHHPHPHLPSHFTNYPS 65

Query: 68  -DIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDS 127
             I  AC +TRFPDQCGSS+S S+ LPPNP+PLQILQ+A++LSS+ L TAQS+V+ ILDS
Sbjct: 66  DQILHACKSTRFPDQCGSSLSHSS-LPPNPSPLQILQSAIALSSDGLTTAQSLVKQILDS 125

Query: 128 SADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKN 187
           SA + NR+ AA  CLE L  S+YRISL NDA+  GR KDARAWLSASLLY   C S LK 
Sbjct: 126 SATNLNRTTAAKNCLEVLRNSDYRISLTNDALHHGRTKDARAWLSASLLYHYDCWSALKY 185

Query: 188 VNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGS 247
            NDT+ V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G  G 
Sbjct: 186 ANDTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGSGL 245

Query: 248 AFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRV 307
            F  GGFP+++ ADATVCKDGSGGCY ++Q+AVDA+PENAAARRFVIHIKEGVYEEIVRV
Sbjct: 246 GF-SGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRV 305

Query: 308 PLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDA 367
           PL+KKNVVF GDGMGKTVI+GSLNVGQPG+STYN+AT+GV GDGFMASGLTI+NTAGPDA
Sbjct: 306 PLEKKNVVFLGDGMGKTVITGSLNVGQPGLSTYNTATLGVVGDGFMASGLTIQNTAGPDA 365

Query: 368 HQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCH 427
           HQAVAFRSDSDLSVIQ+CEF+GNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC 
Sbjct: 366 HQAVAFRSDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCE 425

Query: 428 ILLRPR----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFL 487
           IL+RPR      G +  VTAHGRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ FL
Sbjct: 426 ILVRPRQLKPEKGENNAVTAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPQVHKNFL 485

Query: 488 GRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSS 547
           GRPWKEYSRTVFIHC+LEA+VSP GW PWSG+FAL TLFYGEFEN G GS  SERVGWSS
Sbjct: 486 GRPWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGAGSKTSERVGWSS 545

Query: 548 QIPAEYVSTYSVENFIQGNEWIPLS 550
           QIPA++V +YSVENFIQG+EWIP S
Sbjct: 546 QIPAQHVFSYSVENFIQGDEWIPSS 568

BLAST of Moc02g17520 vs. ExPASy TrEMBL
Match: A0A5A7TBR9 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00730 PE=3 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 3.4e-223
Identity = 383/503 (76.14%), Postives = 434/503 (86.28%), Query Frame = 0

Query: 51  IAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSA 110
           I  AC +TRFPDQCGSS+S S+ LPPNP+PLQILQ+A++LSS+ L TAQS+V+ ILDSSA
Sbjct: 42  ILHACKSTRFPDQCGSSLSHSS-LPPNPSPLQILQSAIALSSDGLTTAQSLVKQILDSSA 101

Query: 111 DSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVN 170
            + NR+ AA  CLE L  S+YRISL NDA+ +GR KDARAWLSASLLY   C S LK  N
Sbjct: 102 TNLNRTTAAKNCLEVLRNSDYRISLTNDALHRGRTKDARAWLSASLLYHYDCWSALKYAN 161

Query: 171 DTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGSAF 230
           DT+ V  TM+FLD LIGLSSNALSM VAYD+YGN+TA W PPRTERDGFWEG G G    
Sbjct: 162 DTQLVDKTMAFLDSLIGLSSNALSMLVAYDIYGNDTAAWTPPRTERDGFWEGGGGGSGLG 221

Query: 231 VGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPL 290
             GGFP+++ ADATVCKDGSGGCY ++Q+AVDA+PENAAARRFVIHIKEGVYEEIVRVPL
Sbjct: 222 FSGGFPAKVTADATVCKDGSGGCYGSIQKAVDAAPENAAARRFVIHIKEGVYEEIVRVPL 281

Query: 291 KKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQ 350
           +KKNVVF GDGMGKTVI+GSLN GQPGISTYN+AT+GV GDGFMASGLTI+NTAGPDAHQ
Sbjct: 282 EKKNVVFLGDGMGKTVITGSLNAGQPGISTYNTATLGVLGDGFMASGLTIQNTAGPDAHQ 341

Query: 351 AVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHIL 410
           AVAFRSDSDLSVIQ+CEFIGNQDTLYAHSLRQFY SC+I GNVDFIFGNSASIFQDC IL
Sbjct: 342 AVAFRSDSDLSVIQDCEFIGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASIFQDCEIL 401

Query: 411 LRPR----PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGR 470
           +RPR      G +  +TAHGRTDPAQSTGFVF NCL+NGT+E+MSLYY+ P+VH+ FLGR
Sbjct: 402 IRPRQLKPEKGENNAITAHGRTDPAQSTGFVFQNCLINGTQEYMSLYYSKPKVHKNFLGR 461

Query: 471 PWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQI 530
           PWKEYSRTVFIHC+LEA+VSP GW PWSG+FAL TLFYGEFEN G+GS  SERVGWSSQI
Sbjct: 462 PWKEYSRTVFIHCNLEALVSPSGWMPWSGDFALKTLFYGEFENSGVGSKTSERVGWSSQI 521

Query: 531 PAEYVSTYSVENFIQGNEWIPLS 550
           PA++V +YSV+NFIQG+EWIP S
Sbjct: 522 PAQHVFSYSVQNFIQGDEWIPSS 543

BLAST of Moc02g17520 vs. ExPASy TrEMBL
Match: A0A0A0LMY9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302140 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.4e-221
Identity = 398/534 (74.53%), Postives = 447/534 (83.71%), Query Frame = 0

Query: 23  MVSLLAL-SLSLYLSSATHHRRLQSLRPDIAAACAATRFPDQCGSSISQSNRLPPNPTPL 82
           M SLL L  L L+ SSA H    Q+L PDIAAAC ATRFPDQC SSISQS+RLP NPT L
Sbjct: 1   MPSLLFLFFLPLHFSSAAH----QTLLPDIAAACNATRFPDQCASSISQSSRLPQNPTSL 60

Query: 83  QILQAAVSLSSENLRTAQSMVQAILDSS--ADSRNRSAAALACLEALGYSEYRISLANDA 142
           QI+QAA+ LSS+NL+TA+SMV+AILDS+    +RNR+  AL+C++ALGYSEYRISL+NDA
Sbjct: 61  QIIQAALYLSSDNLKTAKSMVKAILDSAEKIPNRNRTEKALSCMDALGYSEYRISLSNDA 120

Query: 143 VPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTMSFLDYLIGLSSNALSMAVAY 202
           +P GR KDARAWLSASLLYQ+GCRSGLK VNDTR V  TM FLD L  LSSNALSM VAY
Sbjct: 121 IPLGRIKDARAWLSASLLYQTGCRSGLKYVNDTREVDQTMLFLDNLTVLSSNALSMVVAY 180

Query: 203 DVYGNETAVWRPPRTERDGFWEGSGPGGSAFVGGGFPSELAADATVCKDGSGGCYKTVQE 262
           D +GNETA WRPPRTERDGFWE S   G    G   P++  A+ TVCKDGS GCY+TVQ 
Sbjct: 181 DNFGNETAAWRPPRTERDGFWERSSGTGEVVRGVRIPTKTEANVTVCKDGSEGCYRTVQA 240

Query: 263 AVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGMGKTVISGSLNVGQPGIS 322
           AVDA+P  +A  RFVIHIKEGVYEE VRV ++KKN+VF GDGMGKTVI+GSLNVGQPGIS
Sbjct: 241 AVDAAPGESAV-RFVIHIKEGVYEESVRVGMEKKNLVFLGDGMGKTVITGSLNVGQPGIS 300

Query: 323 TYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHS 382
           TYNSATVGV GDGF+AS LTI+NTAGPDAHQAVAFRSDSDLS+I NCEFI NQDTLYAHS
Sbjct: 301 TYNSATVGVLGDGFIASELTIQNTAGPDAHQAVAFRSDSDLSIIDNCEFISNQDTLYAHS 360

Query: 383 LRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPR----PTGGDYPVTAHGRTDPAQSTG 442
           LRQFYNSCRILGNVDFIFGNSAS+F +CHIL+RP       G +  +TAHGRTDPAQSTG
Sbjct: 361 LRQFYNSCRILGNVDFIFGNSASMFLNCHILIRPHQLNPQLGDENAITAHGRTDPAQSTG 420

Query: 443 FVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSG 502
           FVF NCL+NGTEEFM+LYYA+P+ HRTFLGRPWKEYSRTVFIHC+LEA+VS  GW  WSG
Sbjct: 421 FVFQNCLINGTEEFMALYYANPQRHRTFLGRPWKEYSRTVFIHCELEALVSSSGWMAWSG 480

Query: 503 EFALNTLFYGEFENFGIGSNLSERVGWSSQIPAEYVSTYSVENFIQGNEWIPLS 550
           +  LNTL+YGEFENFG GSN S+RV WS+QIPAEYV +YSV+NFIQGNEWIP S
Sbjct: 481 DIGLNTLYYGEFENFGTGSNSSKRVAWSNQIPAEYVFSYSVQNFIQGNEWIPSS 529

BLAST of Moc02g17520 vs. ExPASy TrEMBL
Match: A0A6J1EV58 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1)

HSP 1 Score: 776.9 bits (2005), Expect = 5.5e-221
Identity = 392/558 (70.25%), Postives = 453/558 (81.18%), Query Frame = 0

Query: 11  FPLSRSASLLISMVSLLALS-LSLY----LSSATHHRRLQSLRP----------DIAAAC 70
           F  ++S  L +SM +L  LS LSL+    LSSA HHR      P          +   AC
Sbjct: 17  FNAAKSTFLFLSMAALSFLSFLSLFFFLSLSSAVHHRHHHHHLPSHFTNSPSSEETFRAC 76

Query: 71  AATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNR 130
            ATRFPDQCGSS+S S  LPPNPT LQI+Q+A+++SS+ L +A+SMV+ ILDSS DS NR
Sbjct: 77  KATRFPDQCGSSLSHSPSLPPNPTTLQIIQSAIAVSSDGLASAKSMVKQILDSSTDSPNR 136

Query: 131 SAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAV 190
           + AA  CLE L +SEYRISL NDAV +GR KDARAW+SASLLY   C S LK  NDT++V
Sbjct: 137 TTAAKNCLEVLRFSEYRISLTNDAVRRGRTKDARAWMSASLLYHYDCWSALKYANDTQSV 196

Query: 191 GDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGSAFVGGGF 250
             TM+FLD LIGL+SNALSM  AYD+ GN+TA W PPRTERDGFWEGS  G      GGF
Sbjct: 197 NKTMAFLDSLIGLTSNALSMLAAYDISGNDTAAWTPPRTERDGFWEGSSGGSGLGFSGGF 256

Query: 251 PSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNV 310
           PS++ A ATVCKDG+GGCYKTVQEAV+A+P+NA  +RFVIHIKEGVY+EIVRVPL+KKNV
Sbjct: 257 PSKVDASATVCKDGAGGCYKTVQEAVNAAPDNAMGKRFVIHIKEGVYDEIVRVPLEKKNV 316

Query: 311 VFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFR 370
           VF GDGMGKTVI+GSLNVGQPGISTYN+ATVGV GDGFMASGLTI+NTAGPDAHQAVAFR
Sbjct: 317 VFLGDGMGKTVITGSLNVGQPGISTYNTATVGVVGDGFMASGLTIQNTAGPDAHQAVAFR 376

Query: 371 SDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPR- 430
           SDSDLSVIQ+CEF+GNQDTLYAHSLRQFY SC+I GNVDFIFGNSAS+FQDC IL+RPR 
Sbjct: 377 SDSDLSVIQDCEFLGNQDTLYAHSLRQFYKSCKIQGNVDFIFGNSASVFQDCEILVRPRQ 436

Query: 431 ---PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEY 490
                G +  VTAHGRTDPAQ+TGFVF NCLVNGT+E+M+LYY+ P+VH+ FLGRPWKEY
Sbjct: 437 LKPEKGENNAVTAHGRTDPAQTTGFVFQNCLVNGTQEYMALYYSKPKVHKNFLGRPWKEY 496

Query: 491 SRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQIPAEYV 550
           SRTVFIHC+LEA+VSP GW PW+G+FAL TL+YGEFEN G GS  SERV WSSQIPA++V
Sbjct: 497 SRTVFIHCNLEALVSPAGWMPWTGDFALKTLYYGEFENSGTGSKTSERVEWSSQIPAQHV 556

BLAST of Moc02g17520 vs. TAIR 10
Match: AT5G09760.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 656.0 bits (1691), Expect = 2.7e-188
Identity = 336/552 (60.87%), Postives = 411/552 (74.46%), Query Frame = 0

Query: 23  MVSLLALSLSLYL-----SSATHHRR-----------LQSLRPDIAAACAATRFPDQCGS 82
           M S+L L  SL+L     SS+ HH               SL   I  AC ATR+PDQC S
Sbjct: 1   MSSILILLFSLFLFSSPSSSSRHHHHHNSGDTSPVNPSSSLAAQIRLACNATRYPDQCVS 60

Query: 83  SISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEAL 142
           S+S+  R+PP+P P+QI+ +A+S S +NL+TAQS +++I+DSS  + NR+ AA  CL+ L
Sbjct: 61  SLSEQGRVPPDPKPIQIIHSAISFSFQNLKTAQSKIKSIVDSSVGNLNRTNAANTCLQLL 120

Query: 143 GYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTMSFLDYLI 202
            YSE+R    + A+ +G+ KDARAW+SA+L+YQ    S LK VNDT  VG+TMSFLD LI
Sbjct: 121 TYSEHRTQSTDQALTRGKIKDARAWMSAALVYQYDSWSALKYVNDTSQVGETMSFLDGLI 180

Query: 203 GLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPG----GSAFVGGGFPSELAAD 262
            ++SNALSM V+YD +G+  A W  P TERDGFWE +GPG     S  +  GFPS L  D
Sbjct: 181 HVTSNALSMMVSYDNFGDNVASWTYPATERDGFWEKTGPGLGLDPSTGLNLGFPSGLKED 240

Query: 263 ATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGM 322
            TVCKDG  G YKTVQ+AV+A+PE+   R+FVI I EGVYEE V VP +KKNVVF GDGM
Sbjct: 241 VTVCKDGKCG-YKTVQDAVNAAPEDNGMRKFVIKISEGVYEENVIVPFEKKNVVFIGDGM 300

Query: 323 GKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSV 382
           GKTVI+GSLN G PGI+TYN+ATVGV GDGFMA  LT +NTAGPDAHQAVAFRSDSD S+
Sbjct: 301 GKTVITGSLNAGMPGITTYNTATVGVVGDGFMARDLTFQNTAGPDAHQAVAFRSDSDFSL 360

Query: 383 IQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPR----PTGG 442
           I+NCEF+GNQDTLYAH LRQFY +CRI GNVDFIFGNSA++FQDC IL+ PR      G 
Sbjct: 361 IENCEFLGNQDTLYAHGLRQFYKNCRIQGNVDFIFGNSAAVFQDCEILIAPRQINPEKGE 420

Query: 443 DYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIH 502
              VTA GR DP+QSTGFVFLNCL+NGTEE+M L+ A+P+VH+ FLGRPWK+YSRTVFI 
Sbjct: 421 KNAVTAQGRIDPSQSTGFVFLNCLINGTEEYMKLFKANPKVHKNFLGRPWKDYSRTVFIG 480

Query: 503 CDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSSQIPAEYVSTYSVEN 551
           C+LEA+++P GW PWSG+FAL TL+YGE +N G GS+ S+RV WSSQIP E+V  YSV N
Sbjct: 481 CNLEALITPDGWLPWSGDFALKTLYYGESKNTGPGSDRSQRVSWSSQIPDEHVHVYSVAN 540

BLAST of Moc02g17520 vs. TAIR 10
Match: AT5G64640.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 627.1 bits (1616), Expect = 1.3e-179
Identity = 324/567 (57.14%), Postives = 408/567 (71.96%), Query Frame = 0

Query: 13  LSRSASLLI---SMVSLLALSLSLYL----SSATHHR--------------RLQSLRPDI 72
           LSR+  L+I   S + LL +SL +Y     SS  HH                     P I
Sbjct: 31  LSRNGILIIIAASCILLLLISLLIYATVSKSSRNHHNPSHQTPTSDDHPPPETPPSPPPI 90

Query: 73  A---AACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDS 132
           A    AC ATRFPD C +S+S+  ++PP+P P+QI+ +A+S+S ENL++ QS +Q+ILDS
Sbjct: 91  AQIRLACNATRFPDHCVASLSKPGQVPPDPKPVQIIHSAISVSYENLKSGQSKIQSILDS 150

Query: 133 SADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKN 192
           SA +RNR+  A  CLE L YS++R    + AV  G  KDARAW+SA+L YQ  C SGLK 
Sbjct: 151 SAGNRNRTNIATICLEILSYSQHRTESTDIAVTSGDIKDARAWMSAALAYQFDCWSGLKT 210

Query: 193 VNDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEGSGPGGS 252
           VNDT+ V DT++F + L+ L+ NALSM +++D +G++   W  P TERDGFWE +GP   
Sbjct: 211 VNDTKQVVDTITFFEGLVNLTGNALSMMLSFDSFGDDVVSWIRPATERDGFWEKAGPSLG 270

Query: 253 AFVGG----GFPSELAADATVCKDGSGGC-YKTVQEAVDASPENAAARRFVIHIKEGVYE 312
           +  G     GFPS L  D TVCK+G   C YKTVQEAVD++P+     +FVI I+EGVYE
Sbjct: 271 SGTGTEASLGFPSGLTEDVTVCKNGGKDCKYKTVQEAVDSAPDTNRTVKFVIRIREGVYE 330

Query: 313 EIVRVPLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENT 372
           E VRVP +KKNVVF GDGMGKTVI+GSLNVGQPG++T+ SATVGV GDGFMA  LTIENT
Sbjct: 331 ETVRVPFEKKNVVFIGDGMGKTVITGSLNVGQPGMTTFESATVGVLGDGFMARDLTIENT 390

Query: 373 AGPDAHQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASI 432
           AG DAHQAVAFRSDSD SV++NCEF+GNQDTLYAHSLRQFY  CRI GNVDFIFGNSA++
Sbjct: 391 AGADAHQAVAFRSDSDFSVLENCEFLGNQDTLYAHSLRQFYKQCRIQGNVDFIFGNSAAV 450

Query: 433 FQDCHILLRPRPT-----GGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPR 492
           FQDC IL+  + +     G +  +TAHGR D +QSTGFVFLNC +NGTEE+M  + A+P 
Sbjct: 451 FQDCDILIASKHSKLEQGGANNAITAHGRIDASQSTGFVFLNCSINGTEEYMKEFQANPE 510

Query: 493 VHRTFLGRPWKEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSE 546
            H+ FLGRPWKE+SRTVF++C+LE+++SP GW PW+G+FAL TL+YGE++N G GS  S 
Sbjct: 511 GHKNFLGRPWKEFSRTVFVNCNLESLISPDGWMPWNGDFALKTLYYGEYKNTGPGSVRSS 570

BLAST of Moc02g17520 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 312.4 bits (799), Expect = 7.3e-85
Identity = 191/504 (37.90%), Postives = 279/504 (55.36%), Query Frame = 0

Query: 50  DIAAACAATRFPDQCGSSISQSNRLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSS 109
           D+ A C+ T  P  C   ++ ++   P  +  + L+ ++ L  +    A++    +    
Sbjct: 29  DVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKC 88

Query: 110 ADSRNRSAAALACLEALGYSEYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNV 169
            D+R + AA   C++    +  +I+   D   +    DA+ WLS +L     CR+G   +
Sbjct: 89  RDTREK-AAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQTWLSTALTNLDTCRAGFLEL 148

Query: 170 NDTRAVGDTMSFLDYLIGLSSNALSMAVAYDVYGNETAV-WRPPRTERDGFWEGSGPGGS 229
             T          D ++ L SN +S  +   +  N+    + PP  E+DGF     PG  
Sbjct: 149 GVT----------DIVLPLMSNNVSNLLCNTLAINKVPFNYTPP--EKDGFPSWVKPGDR 208

Query: 230 AFVGGGFPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRV 289
             +    P +   +A V KDGSG  +KT++EA+DA+   + + RFVI++K+GVY E   +
Sbjct: 209 KLLQSSTPKD---NAVVAKDGSGN-FKTIKEAIDAA---SGSGRFVIYVKQGVYSE--NL 268

Query: 290 PLKKKNVVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDA 349
            ++KKNV+  GDG+GKT+I+GS +VG  G +T+NSATV   GDGF+A G+T  NTAG   
Sbjct: 269 EIRKKNVMLRGDGIGKTIITGSKSVG-GGTTTFNSATVAAVGDGFIARGITFRNTAGASN 328

Query: 350 HQAVAFRSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCH 409
            QAVA RS SDLSV   C F   QDTLY HS RQFY  C + G VDFIFGN+A++ Q+C+
Sbjct: 329 EQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCN 388

Query: 410 ILLRPRPTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPW 469
           I  R RP      +TA GR+DP Q+TG +  N  V    +   +  +     +T+LGRPW
Sbjct: 389 IFAR-RPRSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGST----KTYLGRPW 448

Query: 470 KEYSRTVFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWSS---Q 529
           ++YSRTVF+   L++++ P GW  W G FAL TLFY EF+N G G++ S RV W      
Sbjct: 449 RQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVL 504

Query: 530 IPAEYVSTYSVENFIQGNEWIPLS 550
             A   S ++V  F+ G  WIP S
Sbjct: 509 GSASEASKFTVGTFLAGGSWIPSS 504

BLAST of Moc02g17520 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 303.1 bits (775), Expect = 4.4e-82
Identity = 191/547 (34.92%), Postives = 290/547 (53.02%), Query Frame = 0

Query: 13  LSRSASLLISMVSLLALSLSLYLSSATHHRRLQSLRPDIAAACAATRFPDQCGSSISQSN 72
           +S S+ LLISMV  + + +S+  S       + +    I   CA T + + C  ++ +  
Sbjct: 19  ISISSVLLISMVVAVTIGVSVNKSDNAGDEEITTSVKAIKDVCAPTDYKETCEDTLRKDA 78

Query: 73  RLPPNPTPLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEALGYSEYR 132
           +      PL++++ A + + + +       Q +++   D R + A    C E + Y+   
Sbjct: 79  K--DTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRAKMALD-QCKELMDYAIGE 138

Query: 133 ISLANDAVPQGRNKDA-------RAWLSASLLYQSGCRSGLKNVNDTRAVGDTM-SFLDY 192
           +S + + + +             R WLSA++ ++  C  G +        G+T+   L  
Sbjct: 139 LSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQGTQGN--AGETIKKALKT 198

Query: 193 LIGLSSNALSMAVAYDVYGNETAV--WRPPRTERDGFWEGSGPGGSAFVGGGFPSELAAD 252
            + L+ N L+M      Y  +  +      R     F           +     SE+  D
Sbjct: 199 AVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLSQEFPSWMDARARRLLNAPM-SEVKPD 258

Query: 253 ATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKNVVFFGDGM 312
             V +DGSG  YKT+ EA++  P+      FV+HIKEG+Y+E V+V     ++VF GDG 
Sbjct: 259 IVVAQDGSGQ-YKTINEALNFVPKKKNT-TFVVHIKEGIYKEYVQVNRSMTHLVFIGDGP 318

Query: 313 GKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAFRSDSDLSV 372
            KTVISGS +  + GI+TY +ATV + GD F+A  +  ENTAG   HQAVA R  +D S+
Sbjct: 319 DKTVISGSKSY-KDGITTYKTATVAIVGDHFIAKNIAFENTAGAIKHQAVAIRVLADESI 378

Query: 373 IQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPRPTGGDYPV 432
             NC+F G QDTLYAHS RQFY  C I G +DF+FG++A++FQ+C +L+R        P+
Sbjct: 379 FYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAVFQNCTLLVRKPLLNQACPI 438

Query: 433 TAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRTVFIHCDLE 492
           TAHGR DP +STGFV   C + G  +    Y A     +T+LGRPWKEYSRT+ ++  + 
Sbjct: 439 TAHGRKDPRESTGFVLQGCTIVGEPD----YLAVKEQSKTYLGRPWKEYSRTIIMNTFIP 498

Query: 493 AVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWS--SQIPAEYVSTYSVENFI 548
             V P GW PW GEF LNTLFY E +N G G+ +++RV W    ++  E +  ++   +I
Sbjct: 499 DFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKKLSDEEILKFTPAQYI 552

BLAST of Moc02g17520 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 298.5 bits (763), Expect = 1.1e-80
Identity = 193/555 (34.77%), Postives = 293/555 (52.79%), Query Frame = 0

Query: 16  SASLLISMVSLLALSLSL--YLSSATHHRRLQSLRPDIAAACAATRFPDQCGSSISQSNR 75
           S+ LLISMV  + + +SL  +   +     + +    +   CA T +   C  ++ ++ +
Sbjct: 21  SSVLLISMVVAVTVGVSLNKHDGDSKGKAEVNASVKAVKDVCAPTDYRKTCEDTLIKNGK 80

Query: 76  LPPNPT-PLQILQAAVSLSSENLRTAQSMVQAILDSSADSRNRSAAALACLEALGYS--- 135
              N T P+++++ A +++ + +  A    Q I++   DSR R A    C E + Y+   
Sbjct: 81  ---NTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSRTRMALD-QCKELMDYALDE 140

Query: 136 ---------EYRISLANDAVPQGRNKDARAWLSASLLYQSGCRSGLKNVNDTRAVGDTM- 195
                    ++   L ++A+      + R WLSA++ ++  C  G +        G+TM 
Sbjct: 141 LSNSFEELGKFEFHLLDEAL-----INLRIWLSAAISHEETCLEGFQGTQGN--AGETMK 200

Query: 196 SFLDYLIGLSSNALSMAVAYDVYGNETAVWRPPRTERDGFWEG-----SGPGGSAFVGGG 255
             L   I L+ N L++      +  +  +  P    R    EG        G        
Sbjct: 201 KALKTAIELTHNGLAIISEMSNFVGQMQI--PGLNSRRLLAEGFPSWVDQRGRKLLQAAA 260

Query: 256 FPSELAADATVCKDGSGGCYKTVQEAVDASPENAAARRFVIHIKEGVYEEIVRVPLKKKN 315
             S++  D  V +DGSG  YKT+ EA+   P+      FV+HIK G+Y+E V+V     +
Sbjct: 261 AYSDVKPDIVVAQDGSGQ-YKTINEALQFVPKKRNT-TFVVHIKAGLYKEYVQVNKTMSH 320

Query: 316 VVFFGDGMGKTVISGSLNVGQPGISTYNSATVGVAGDGFMASGLTIENTAGPDAHQAVAF 375
           +VF GDG  KT+ISG+ N  + GI+TY +ATV + G+ F+A  +  ENTAG   HQAVA 
Sbjct: 321 LVFIGDGPDKTIISGNKNY-KDGITTYRTATVAIVGNYFIAKNIGFENTAGAIKHQAVAV 380

Query: 376 RSDSDLSVIQNCEFIGNQDTLYAHSLRQFYNSCRILGNVDFIFGNSASIFQDCHILLRPR 435
           R  SD S+  NC F G QDTLY HS RQF+  C I G +DF+FG++A++FQ+C +L+R  
Sbjct: 381 RVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAAAVFQNCTLLVRKP 440

Query: 436 PTGGDYPVTAHGRTDPAQSTGFVFLNCLVNGTEEFMSLYYADPRVHRTFLGRPWKEYSRT 495
                 P+TAHGR DP +STGFVF  C + G  +    Y A     + +LGRPWKEYSRT
Sbjct: 441 LPNQACPITAHGRKDPRESTGFVFQGCTIAGEPD----YLAVKETSKAYLGRPWKEYSRT 500

Query: 496 VFIHCDLEAVVSPFGWSPWSGEFALNTLFYGEFENFGIGSNLSERVGWS--SQIPAEYVS 548
           + ++  +   V P GW PW G+F L TLFY E +N G GS L+ RV W+    +  E + 
Sbjct: 501 IIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGIKTLSEEDIL 555

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038900555.18.0e-23577.37probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida][more]
XP_038902126.11.6e-22773.39probable pectinesterase/pectinesterase inhibitor 51 [Benincasa hispida][more]
XP_008460902.18.9e-22670.67PREDICTED: LOW QUALITY PROTEIN: probable pectinesterase/pectinesterase inhibitor... [more]
XP_004147375.22.0e-22571.86probable pectinesterase/pectinesterase inhibitor 51 [Cucumis sativus] >KGN62146.... [more]
KAA0040770.17.1e-22376.14putative pectinesterase/pectinesterase inhibitor 51 [Cucumis melo var. makuwa] >... [more]
Match NameE-valueIdentityDescription
Q9LXD93.8e-18760.87Probable pectinesterase/pectinesterase inhibitor 51 OS=Arabidopsis thaliana OX=3... [more]
Q8L7Q71.9e-17857.14Probable pectinesterase/pectinesterase inhibitor 64 OS=Arabidopsis thaliana OX=3... [more]
O048872.9e-8638.04Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221491.0e-8337.90Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q3E8Z86.3e-8134.92Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
A0A1S3CD054.3e-22670.67Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499643 PE=3 SV=1[more]
A0A0A0LQ809.6e-22671.86Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302130 PE=3 SV=1[more]
A0A5A7TBR93.4e-22376.14Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold680G00730... [more]
A0A0A0LMY91.4e-22174.53Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G302140 PE=3 SV=1[more]
A0A6J1EV585.5e-22170.25Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111438335 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G09760.12.7e-18860.87Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G64640.11.3e-17957.14Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.17.3e-8537.90Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G27870.14.4e-8234.92Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G05610.11.1e-8034.77Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 46..195
e-value: 1.5E-14
score: 64.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 51..195
e-value: 4.1E-18
score: 66.1
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 24..193
e-value: 7.2E-15
score: 52.7
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 48..198
e-value: 6.9E-20
score: 73.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 50..195
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 242..543
e-value: 5.1E-117
score: 389.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 236..550
e-value: 6.3E-107
score: 359.6
NoneNo IPR availablePANTHERPTHR31707:SF249PECTINESTERASE/PECTINESTERASE INHIBITORcoord: 29..548
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 29..548
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 53..195
e-value: 1.92159E-17
score: 77.4785
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 239..548

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g17520.1Moc02g17520.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity