Moc02g15610 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g15610
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionPoly(A)-specific ribonuclease
Locationchr2: 11749573 .. 11750760 (-)
RNA-Seq ExpressionMoc02g15610
SyntenyMoc02g15610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAACAGCACAATTCCCAATCAAAGTAGCGGAAATACATTTAGAGTGAAGAAGTCACTGGAAAGAATTGAGGTGACTATTGGAAAGTTACCAGCAACTCCAAGATTCATCCACCAGAATCGAGAGGTTTGGTCTTACAATCTCTGCAAAGAGTTACAGCTGCTGAATGAGTATCTTCTAGAGTTTCAGCAGTCGTCATTGATACAGAGTTTTCAGGTATTGAGTTTCTTAAATCAGGTGGATAATCGAAACTATTTTTTAAGTTTAGCTAGAAAAGGTTTTGACGCTCTTGAGCTCGATCGATGAACCAGAATTTCTTTGATACACTCCGAAAGGTGTTTAGAGGAAGAATTCTATGATTGTCACTGATTCAACGTGAACCGATTGAACACACTCCAACTTGATCTCACACTAGTCGATCAAAATGGACATGTAGTGATGATTTGGAAATTTCATTTTAGAGATTTTGATCACATTAGCGTCATAGAATGCGTTTCTTCACTAGATCCGTTTATGAAGAACAAGGGATTCGATTTCTACAAATTGAAGAAAGACAGAATAGCTACAGAGGATTTCACTGCCATGTTTCTACCGATTTGTCGTAGTGGTAGAATCGAGAGATGGATCACATTTCATGACTTCTACGACATCGCTTATCTTTTGAAGCTGCTCAAAATCAAATCCATACAATTTCTATGGCAACGTTTGTTGTCATAGCTCAGCATCTATTAGGTACTAATGAATCGTCAATCAATTTAAAAGCTTAATTTGATATATTATGGTAAATTTAATATGTTATATTAACTTAATAGGTACTATCAGCGATTTGAAGCATATAGCTCGCTATTGCGATGGATTGCTCGATTGCGACCTAAGGCTAAAAAATTTTGTCAAGCTCCTCGACGGGAAACAAATAAGCATAACACATTCACCGATTCTGATAGTTTACTCACAACTGCAGTATCCACCAAGATGAAGCAAATGTTCAACCTTCCGGTAGAGCAATGTGAAGGGTTTCTCTACGGCTTACCTTACCGGATTCAAGCTTTTCAGCCAATCATGAAGATCATCGATCCGTCCGCCACGACTCCTCCATGTCTGCAACCAAAGTGCTTGATGTTGTCTTTTTGCAGCTTGCCACTGTTTTTCATTGATACTCGATCTAGTAGGTTAATGGTCATTTGTTGA

mRNA sequence

ATGAACAGCACAATTCCCAATCAAAGTAGCGGAAATACATTTAGAGTGAAGAAGTCACTGGAAAGAATTGAGGTGACTATTGGAAAGTTACCAGCAACTCCAAGATTCATCCACCAGAATCGAGAGGTTTGGTCTTACAATCTCTGCAAAGAGTTACAGCTGCTGAATGAGTATCTTCTAGAGTTTCAGCAGTCGTCATTGATACAGAGTTTTCAGAACAAGGGATTCGATTTCTACAAATTGAAGAAAGACAGAATAGCTACAGAGGATTTCACTGCCATCTGCTCAAAATCAAATCCATACAATTTCTATGGCAACGTTTGTTGTCATAGCTCAGCATCTATTAGGTACTATCAGCGATTTGAAGCATATAGCTCGCTATTGCGATGGATTGCTCGATTGCGACCTAAGGCTAAAAAATTTTGTCAAGCTCCTCGACGGGAAACAAATAAGCATAACACATTCACCGATTCTGATAGTTTACTCACAACTGCAGTATCCACCAAGATGAAGCAAATGTTCAACCTTCCGGTAGAGCAATGTGAAGGGTTTCTCTACGGCTTACCTTACCGGATTCAAGCTTTTCAGCCAATCATGAAGATCATCGATCCGTCCGCCACGACTCCTCCATGTCTGCAACCAAAGTGCTTGATGTTGTCTTTTTGCAGCTTGCCACTGTTTTTCATTGATACTCGATCTAGTAGGTTAATGGTCATTTGTTGA

Coding sequence (CDS)

ATGAACAGCACAATTCCCAATCAAAGTAGCGGAAATACATTTAGAGTGAAGAAGTCACTGGAAAGAATTGAGGTGACTATTGGAAAGTTACCAGCAACTCCAAGATTCATCCACCAGAATCGAGAGGTTTGGTCTTACAATCTCTGCAAAGAGTTACAGCTGCTGAATGAGTATCTTCTAGAGTTTCAGCAGTCGTCATTGATACAGAGTTTTCAGAACAAGGGATTCGATTTCTACAAATTGAAGAAAGACAGAATAGCTACAGAGGATTTCACTGCCATCTGCTCAAAATCAAATCCATACAATTTCTATGGCAACGTTTGTTGTCATAGCTCAGCATCTATTAGGTACTATCAGCGATTTGAAGCATATAGCTCGCTATTGCGATGGATTGCTCGATTGCGACCTAAGGCTAAAAAATTTTGTCAAGCTCCTCGACGGGAAACAAATAAGCATAACACATTCACCGATTCTGATAGTTTACTCACAACTGCAGTATCCACCAAGATGAAGCAAATGTTCAACCTTCCGGTAGAGCAATGTGAAGGGTTTCTCTACGGCTTACCTTACCGGATTCAAGCTTTTCAGCCAATCATGAAGATCATCGATCCGTCCGCCACGACTCCTCCATGTCTGCAACCAAAGTGCTTGATGTTGTCTTTTTGCAGCTTGCCACTGTTTTTCATTGATACTCGATCTAGTAGGTTAATGGTCATTTGTTGA

Protein sequence

MNSTIPNQSSGNTFRVKKSLERIEVTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEFQQSSLIQSFQNKGFDFYKLKKDRIATEDFTAICSKSNPYNFYGNVCCHSSASIRYYQRFEAYSSLLRWIARLRPKAKKFCQAPRRETNKHNTFTDSDSLLTTAVSTKMKQMFNLPVEQCEGFLYGLPYRIQAFQPIMKIIDPSATTPPCLQPKCLMLSFCSLPLFFIDTRSSRLMVIC
Homology
BLAST of Moc02g15610 vs. NCBI nr
Match: XP_022154798.1 (probable CCR4-associated factor 1 homolog 6 [Momordica charantia])

HSP 1 Score: 137.5 bits (345), Expect = 1.5e-28
Identity = 118/341 (34.60%), Postives = 138/341 (40.47%), Query Frame = 0

Query: 23  IEVTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEFQ------------------- 82
           +E T GK P  PR +HQNREVWSYNL KEL++LNEYL EF                    
Sbjct: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60

Query: 83  ------------------------------------------------------------ 142
                                                                       
Sbjct: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120

Query: 143 --------------------QSSLIQSFQNKGFDFYKLKKDRIATEDFTA----ICSKSN 202
                                SSL    +NKGFDFYKLK+D IA EDFT     IC    
Sbjct: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180

Query: 203 P------YNFYGNVCCHSSASIRY----YQRFEAYSS-LLRWIARLRPKA---------- 237
                  + FY          I+Y       F A +  LL  ++ L+             
Sbjct: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240

BLAST of Moc02g15610 vs. NCBI nr
Match: XP_022146384.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 132.1 bits (331), Expect = 6.4e-27
Identity = 115/336 (34.23%), Postives = 141/336 (41.96%), Query Frame = 0

Query: 7   NQSSGNTFRVKKSLERIE-VTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEF--- 66
           ++ + N FRV KSLE +E   + K  A PR I  NREVWS NL +EL+LLNEYL EF   
Sbjct: 3   DKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVV 62

Query: 67  ------------------------------------------------------------ 126
                                                                       
Sbjct: 63  VIDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDF 122

Query: 127 -------QQSSLIQSFQNKGFDFYKLKKDRIATEDFTA----ICSKSNPYNFYGNVCCHS 186
                    SSL    +NKGFDFYKLKK  IA EDFT     IC       +   +  H 
Sbjct: 123 DHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKRW---ITFHG 182

Query: 187 SASIRYYQRFEAYSS--------------LLRWIARLRPKAKKFCQA------------- 237
              I Y  +     S              LL  ++ L+  A ++C               
Sbjct: 183 FYDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMA-RYCDGLLDGNLGLKKLAK 242

BLAST of Moc02g15610 vs. NCBI nr
Match: XP_022153955.1 (putative CCR4-associated factor 1 homolog 8 [Momordica charantia])

HSP 1 Score: 112.8 bits (281), Expect = 4.0e-21
Identity = 92/274 (33.58%), Postives = 120/274 (43.80%), Query Frame = 0

Query: 23  IEVTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEF-------------------- 82
           +E TI      PR + + +EVWS+NL +EL+LLNEY+ EF                    
Sbjct: 1   MEKTIRNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTIDIGWYFGRKILRLSP 60

Query: 83  -QQSSLIQ-------------------------------------SFQNKGFDFYKLKKD 142
            Q+ +++Q                                       +N GFDFYKLKKD
Sbjct: 61  IQRLNILQLGLTLVDQKENVAMTLEFNFRDFDHISDMKGISLLDLFLKNNGFDFYKLKKD 120

Query: 143 RIATEDFTA----ICSKSNP------YNFYGNVCCHSSASIRYYQRFEA-----YSSLLR 202
            I  E+FT     IC           + FY          I++   F A        LL 
Sbjct: 121 GIVPENFTTLFLPICHSGRIERLITFHGFYEIAYLLKRLKIKFIPIFMATFAATAQHLLG 180

Query: 203 WIARLRPKA-------------KKFCQAPRRETNKHNTFTDSDSLLTTAVSTKMKQMFNL 211
            I+ L+  A             KKF +    +      F   DSLLT ++ TKMKQMF L
Sbjct: 181 AISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLLTASIYTKMKQMFKL 240

BLAST of Moc02g15610 vs. NCBI nr
Match: XP_038888808.1 (probable CCR4-associated factor 1 homolog 6 [Benincasa hispida])

HSP 1 Score: 60.8 bits (146), Expect = 1.8e-05
Identity = 35/77 (45.45%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 155 FTDSDSLLTTAVSTKMKQMFNLPVEQCEGFLYGLPYRIQAFQPIMKIIDPSATTPPCLQP 214
           F  SDSLLT +V  KMK +F LP +  EG LYGLP+ I+AF+  + I+ PS ++      
Sbjct: 138 FAGSDSLLTASVYAKMKTIFKLPEQLSEGILYGLPHPIRAFRQPVTIVSPSPSS------ 197

Query: 215 KCLMLSFC-SLPLFFID 231
           +CL+ SF  S P+F  D
Sbjct: 198 RCLVSSFSRSSPIFIGD 208

BLAST of Moc02g15610 vs. NCBI nr
Match: XP_022928830.1 (putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata])

HSP 1 Score: 59.7 bits (143), Expect = 4.0e-05
Identity = 51/174 (29.31%), Postives = 71/174 (40.80%), Query Frame = 0

Query: 66  SLIQSFQNKGFDFYKLKKDRIATEDFTA-------------------------------I 125
           SL    +NKGF+F KL+   IA ++F+                                I
Sbjct: 112 SLDSFLKNKGFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNI 171

Query: 126 CSKSNPYNFYGNVCCHSSASIRYYQRFEAYSSLLRWIARLRPKAKKFCQAPRRETNKHNT 185
            S     + +  +  H   ++   +    YS  L        K  K  Q  R  T     
Sbjct: 172 ISMPTSMSMFAMIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAH--- 231

Query: 186 FTDSDSLLTTAVSTKMKQMFNLPVEQCEGFLYGLPYRIQAFQPIMKIIDPSATT 209
           F  SDSLLT +V  KMK +  +  +  EGFLYGLPY IQ ++P + I+DPS  T
Sbjct: 232 FAGSDSLLTASVYAKMKVIMKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLT 282

BLAST of Moc02g15610 vs. ExPASy TrEMBL
Match: A0A6J1DLA5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=3 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 7.3e-29
Identity = 118/341 (34.60%), Postives = 138/341 (40.47%), Query Frame = 0

Query: 23  IEVTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEFQ------------------- 82
           +E T GK P  PR +HQNREVWSYNL KEL++LNEYL EF                    
Sbjct: 1   MEKTFGKSPTAPRSVHQNREVWSYNLHKELEILNEYLPEFPIVAIDTEFPGIGLLKDNRI 60

Query: 83  ------------------------------------------------------------ 142
                                                                       
Sbjct: 61  YFLSLAGKYFYARKLDDEPGFLRYTPKGVSEEEFYDCLRFNVNRLNILQLGLTLANQNGN 120

Query: 143 --------------------QSSLIQSFQNKGFDFYKLKKDRIATEDFTA----ICSKSN 202
                                SSL    +NKGFDFYKLK+D IA EDFT     IC    
Sbjct: 121 VAMTWEFNFRDFDHISDMEGVSSLDSFLKNKGFDFYKLKRDGIALEDFTTLFLPICRSGR 180

Query: 203 P------YNFYGNVCCHSSASIRY----YQRFEAYSS-LLRWIARLRPKA---------- 237
                  + FY          I+Y       F A +  LL  ++ L+             
Sbjct: 181 IERWITFHGFYDIAYLLKLLKIKYIPISMATFAATAQHLLGTVSDLKHMCRYCDGLLDGD 240

BLAST of Moc02g15610 vs. ExPASy TrEMBL
Match: A0A6J1CXZ5 (Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 3.1e-27
Identity = 115/336 (34.23%), Postives = 141/336 (41.96%), Query Frame = 0

Query: 7   NQSSGNTFRVKKSLERIE-VTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEF--- 66
           ++ + N FRV KSLE +E   + K  A PR I  NREVWS NL +EL+LLNEYL EF   
Sbjct: 3   DKRNDNIFRVMKSLENMEKAIVRKSTAAPRSICHNREVWSLNLREELELLNEYLPEFPVV 62

Query: 67  ------------------------------------------------------------ 126
                                                                       
Sbjct: 63  VIDMEFPGFLWCTPKGISEEEFYHCLRFNVNRLNILQLGLTLADQKGNIAMTWEFNFRDF 122

Query: 127 -------QQSSLIQSFQNKGFDFYKLKKDRIATEDFTA----ICSKSNPYNFYGNVCCHS 186
                    SSL    +NKGFDFYKLKK  IA EDFT     IC       +   +  H 
Sbjct: 123 DHVSDMEDVSSLDSFLKNKGFDFYKLKKYGIAQEDFTTLFLPICRSGQIKRW---ITFHG 182

Query: 187 SASIRYYQRFEAYSS--------------LLRWIARLRPKAKKFCQA------------- 237
              I Y  +     S              LL  ++ L+  A ++C               
Sbjct: 183 FYDITYLLKLLKIKSIPISMATFATTAQHLLGTVSDLKHMA-RYCDGLLDGNLGLKKLAK 242

BLAST of Moc02g15610 vs. ExPASy TrEMBL
Match: A0A6J1DIB1 (putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LOC111021342 PE=4 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.9e-21
Identity = 92/274 (33.58%), Postives = 120/274 (43.80%), Query Frame = 0

Query: 23  IEVTIGKLPATPRFIHQNREVWSYNLCKELQLLNEYLLEF-------------------- 82
           +E TI      PR + + +EVWS+NL +EL+LLNEY+ EF                    
Sbjct: 1   MEKTIRNSMPAPRSVRKKQEVWSHNLHEELELLNEYIPEFLVVTIDIGWYFGRKILRLSP 60

Query: 83  -QQSSLIQ-------------------------------------SFQNKGFDFYKLKKD 142
            Q+ +++Q                                       +N GFDFYKLKKD
Sbjct: 61  IQRLNILQLGLTLVDQKENVAMTLEFNFRDFDHISDMKGISLLDLFLKNNGFDFYKLKKD 120

Query: 143 RIATEDFTA----ICSKSNP------YNFYGNVCCHSSASIRYYQRFEA-----YSSLLR 202
            I  E+FT     IC           + FY          I++   F A        LL 
Sbjct: 121 GIVPENFTTLFLPICHSGRIERLITFHGFYEIAYLLKRLKIKFIPIFMATFAATAQHLLG 180

Query: 203 WIARLRPKA-------------KKFCQAPRRETNKHNTFTDSDSLLTTAVSTKMKQMFNL 211
            I+ L+  A             KKF +    +      F   DSLLT ++ TKMKQMF L
Sbjct: 181 AISNLKHMARYCDGLLDGNLALKKFAKLLDVKRIGTAHFAGFDSLLTASIYTKMKQMFKL 240

BLAST of Moc02g15610 vs. ExPASy TrEMBL
Match: A0A6J1ESM7 (Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.9e-05
Identity = 51/174 (29.31%), Postives = 71/174 (40.80%), Query Frame = 0

Query: 66  SLIQSFQNKGFDFYKLKKDRIATEDFTA-------------------------------I 125
           SL    +NKGF+F KL+   IA ++F+                                I
Sbjct: 112 SLDSFLKNKGFNFNKLRSCGIAPQEFSRQFKPVIRSGRIQKWITFHGLYDIAYLMKFNNI 171

Query: 126 CSKSNPYNFYGNVCCHSSASIRYYQRFEAYSSLLRWIARLRPKAKKFCQAPRRETNKHNT 185
            S     + +  +  H   ++   +    YS  L        K  K  Q  R  T     
Sbjct: 172 ISMPTSMSMFAMIAAHLLGTVTDLKHMARYSDYLFNGNLGLKKLAKLLQINRVGTAH--- 231

Query: 186 FTDSDSLLTTAVSTKMKQMFNLPVEQCEGFLYGLPYRIQAFQPIMKIIDPSATT 209
           F  SDSLLT +V  KMK +  +  +  EGFLYGLPY IQ ++P + I+DPS  T
Sbjct: 232 FAGSDSLLTASVYAKMKVIMKVEPKIHEGFLYGLPYPIQGYRPPVVIVDPSKLT 282

BLAST of Moc02g15610 vs. ExPASy TrEMBL
Match: A0A6J1IIN1 (Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111474351 PE=3 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.8e-04
Identity = 39/120 (32.50%), Postives = 58/120 (48.33%), Query Frame = 0

Query: 91  FTAICSKSNPYNFYGNVCCHSSASIRYYQRFEAYSS-LLRWIARLRPKAKKFCQAPRRET 150
           FT+I       + +  +  H   ++   +    YS  LL     L+  AK        + 
Sbjct: 168 FTSIICMPTSMSIFAIMAAHLLGTVTDLKHMARYSDYLLNGNLGLKKLAKML------QV 227

Query: 151 NKHNT--FTDSDSLLTTAVSTKMKQMFNLPVEQCEGFLYGLPYRIQAFQPIMKIIDPSAT 208
           N+  T  F  SDSLLT +V  KM+ +  +  +  EGFLYGLPY IQ ++P + I+DP  T
Sbjct: 228 NRIGTAHFAGSDSLLTASVYAKMRVIMKVDPKIHEGFLYGLPYPIQGYRPPVVIVDPKLT 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022154798.11.5e-2834.60probable CCR4-associated factor 1 homolog 6 [Momordica charantia][more]
XP_022146384.16.4e-2734.23putative CCR4-associated factor 1 homolog 8 [Momordica charantia][more]
XP_022153955.14.0e-2133.58putative CCR4-associated factor 1 homolog 8 [Momordica charantia][more]
XP_038888808.11.8e-0545.45probable CCR4-associated factor 1 homolog 6 [Benincasa hispida][more]
XP_022928830.14.0e-0529.31putative CCR4-associated factor 1 homolog 8 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6J1DLA57.3e-2934.60Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111021965 PE=... [more]
A0A6J1CXZ53.1e-2734.23Poly(A)-specific ribonuclease OS=Momordica charantia OX=3673 GN=LOC111015612 PE=... [more]
A0A6J1DIB11.9e-2133.58putative CCR4-associated factor 1 homolog 8 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1ESM71.9e-0529.31Poly(A)-specific ribonuclease OS=Cucurbita moschata OX=3662 GN=LOC111435626 PE=3... [more]
A0A6J1IIN14.8e-0432.50Poly(A)-specific ribonuclease OS=Cucurbita maxima OX=3661 GN=LOC111474351 PE=3 S... [more]
Match NameE-valueIdentityDescription
Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g15610.1Moc02g15610.1mRNA