Moc02g15300 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g15300
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	RNase H domain-containing protein
Location	chr2: 11389807 .. 11390853 (-)
RNA-Seq Expression	Moc02g15300
Synteny	Moc02g15300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGCCAGAAAGGAGTCCACGACGCTCTGATGATAACTACTCTACCAAAAGGAGGCTGAACTTGGACGACCCCCAGGTTGGAGGATCCGAGGATGGGACCAGCCAGTCAAACCCGGAACGTCAGGAGGGGTTGCCTGAGGCGCATGCAATAACGACCCTTGAGCCACTCCAAAAGCAGTTTGCGGTCTTGGAAGACAAGGTAGAGGACATGCTGCAACAAGAGCCCGACGAGGTACCCCTTGTCAGAGACCCGAGAAAAAGGAAGGGCACAGCGCAAAGCGAGATCGAGGAATCAACAAACAGTACAGGGAGCAAGTTGTGGATAGGTGGAAATACCAGGCGACGAACCCGGATTTTCGACTCCAAGAAGATAAAAAAGCAACATAAATCCCAGAACCAAACGGGGGAAAGAGCAACCAGGATAACCGAAACTCTGAGCCGATAGGCCTCGACAAAGGCAAACCGACAGATCAGCCAGAGTCTTCAGAGAAGCGACAGAACCAAAAGGAGAAGGGATTTGACCTCGAAGAACTACTGGATCAAGCCGACTCACCATTCACGGAGGAGATCATGAAAGAGAAGGTCCCTCCAAAATTCAAGCTACCCACGGTGAAACAATTCGACGGGACGACCGACCCAGTGGACCATCTAGATGCTTATCGGGAATGGATGGATATCTATGGGGTGTCAGAGGCGGTCAGGTGCCGGGTGTTCTCGACTACATTAAACGAGTTAGCAAGAATATGGTTCCAATAATTAAAACGAGGGTCAATCTCGTTTCAAGAGCCTGGCCAGAGCATTCGTGACCCAGTTTGTAGGGGGGCAGTGTTGGAGCCGACCTGTGGCTTATCTTTTAACTATTAAACAGAGGACGACAGAGAGTCTACACGACTATGTAGCTCGGTTCAATGAGGAAAAGCTGTAGGTAGAAGGCCTTACAGACGCCGTATCTCTACTGGCCTTCATGTCCGGCGTCAGGGATGAGCATTTGTCATTTTCGTTAGGAAAGAAGACACCGAGTACCTTTTCAGAGGCGCTGAGCTGA

mRNA sequence

ATGACGCCAGAAAGGAGTCCACGACGCTCTGATGATAACTACTCTACCAAAAGGAGGCTGAACTTGGACGACCCCCAGGTTGGAGGATCCGAGGATGGGACCAGCCAGTCAAACCCGGAACGTCAGGAGGGGTTGCCTGAGGCGCATGCAATAACGACCCTTGAGCCACTCCAAAAGCAGTTTGCGGTCTTGGAAGACAAGGTAGAGGACATGCTGCAACAAGAGCCCGACGAGGTACCCCTTGTCAGAGACCCGAGAAAAAGGAAGGGCACAGCGCAAAGCGAGATCGAGGAATCAACAAACAGTACAGGGAGCAAGTTGTGGATAGGCCTCGACAAAGGCAAACCGACAGATCAGCCAGAGTCTTCAGAGAAGCGACAGAACCAAAAGGAGAAGGGATTTGACCTCGAAGAACTACTGGATCAAGCCGACTCACCATTCACGGAGGAGATCATGAAAGAGAAGGTCCCTCCAAAATTCAAGCTACCCACGGTGAAACAATTCGACGGGACGACCGACCCAGTGGACCATCTAGATGCTTATCGGGAATGGATGGATATCTATGGGGTGTCAGAGGCGGTAGAAGGCCTTACAGACGCCGTATCTCTACTGGCCTTCATGTCCGGCGTCAGGGATGAGCATTTGTCATTTTCGTTAGGAAAGAAGACACCGAGTACCTTTTCAGAGGCGCTGAGCTGA

Coding sequence (CDS)

ATGACGCCAGAAAGGAGTCCACGACGCTCTGATGATAACTACTCTACCAAAAGGAGGCTGAACTTGGACGACCCCCAGGTTGGAGGATCCGAGGATGGGACCAGCCAGTCAAACCCGGAACGTCAGGAGGGGTTGCCTGAGGCGCATGCAATAACGACCCTTGAGCCACTCCAAAAGCAGTTTGCGGTCTTGGAAGACAAGGTAGAGGACATGCTGCAACAAGAGCCCGACGAGGTACCCCTTGTCAGAGACCCGAGAAAAAGGAAGGGCACAGCGCAAAGCGAGATCGAGGAATCAACAAACAGTACAGGGAGCAAGTTGTGGATAGGCCTCGACAAAGGCAAACCGACAGATCAGCCAGAGTCTTCAGAGAAGCGACAGAACCAAAAGGAGAAGGGATTTGACCTCGAAGAACTACTGGATCAAGCCGACTCACCATTCACGGAGGAGATCATGAAAGAGAAGGTCCCTCCAAAATTCAAGCTACCCACGGTGAAACAATTCGACGGGACGACCGACCCAGTGGACCATCTAGATGCTTATCGGGAATGGATGGATATCTATGGGGTGTCAGAGGCGGTAGAAGGCCTTACAGACGCCGTATCTCTACTGGCCTTCATGTCCGGCGTCAGGGATGAGCATTTGTCATTTTCGTTAGGAAAGAAGACACCGAGTACCTTTTCAGAGGCGCTGAGCTGA

Protein sequence

MTPERSPRRSDDNYSTKRRLNLDDPQVGGSEDGTSQSNPERQEGLPEAHAITTLEPLQKQFAVLEDKVEDMLQQEPDEVPLVRDPRKRKGTAQSEIEESTNSTGSKLWIGLDKGKPTDQPESSEKRQNQKEKGFDLEELLDQADSPFTEEIMKEKVPPKFKLPTVKQFDGTTDPVDHLDAYREWMDIYGVSEAVEGLTDAVSLLAFMSGVRDEHLSFSLGKKTPSTFSEALS

Homology

[Close]

BLAST of Moc02g15300 vs. NCBI nr
Match: XP_022158830.1 (uncharacterized protein LOC111025293 [Momordica charantia])

HSP 1 Score: 309.7 bits (792), Expect = 2.1e-80
Identity = 192/349 (55.01%), Postives = 202/349 (57.88%), Query Frame = 0


Query: 1   MTPERSPRRSDDNYSTKRRLNLDDPQVGGSEDGTSQSNPERQEGLPEAHAITTLEPLQKQ 60
           MTPERSPRRSDD+ S KRRLNL DPQVGG EDGTSQ N ERQEGL E  A+TT EP QKQ
Sbjct: 1   MTPERSPRRSDDDCSAKRRLNLGDPQVGGPEDGTSQPNQERQEGLXEXXALTTPEPFQKQ 60

Query: 61  FAVLEDKVEDMLQQEPDEVPLVRDPRKRKGTAQSEIEESTNSTGSKLWIG---------- 120
           FAVLEDK       E DEVPLVRDP+K KG  +S+ EESTNS GSKL IG          
Sbjct: 61  FAVLEDK-------ESDEVPLVRDPKKGKGPTESDTEESTNSVGSKLRIGGNTRQRTRIF 120

Query: 121 ------------------------------LDKGKPTDQPESSEKRQNQKEKGFDLEELL 180
                                         LDKGKP D+PESSEKR + KEKGFDLEELL
Sbjct: 121 DPRKTKKQHKSPAPNGGKSDHDDRNSEPISLDKGKPADRPESSEKRHSHKEKGFDLEELL 180

Query: 181 DQADSPFTEEIMKEKVPPKFKLPTVKQFDGTTDPVDHLDAYREWMDIYGVSEA------- 233
           DQADSPFTEEIM+EKVPPKFKLPTVKQFD TTDPVDHLDAYREWMDIYGVSEA       
Sbjct: 181 DQADSPFTEEIMREKVPPKFKLPTVKQFDXTTDPVDHLDAYREWMDIYGVSEAVRCRVFS 240

[Close]

BLAST of Moc02g15300 vs. NCBI nr
Match: XP_022145129.1 (uncharacterized protein LOC111014646 [Momordica charantia])

HSP 1 Score: 253.1 bits (645), Expect = 2.4e-63
Identity = 150/218 (68.81%), Postives = 152/218 (69.72%), Query Frame = 0


Query: 1   MTPERSPRRSDDNYSTKRRLNLDDPQVGGSEDGTSQSNPERQEGLPEAHAITTLEPLQKQ 60
           MTPERSPRRSDDN S KRRLNLDDPQVGG EDGTSQ N ERQEGLPEAHA+TT EPLQKQ
Sbjct: 1   MTPERSPRRSDDNCSAKRRLNLDDPQVGGPEDGTSQPNLERQEGLPEAHALTTPEPLQKQ 60

Query: 61  FAVLEDKVEDMLQ-----------QEPDEVPLVRDPRKRKGTAQSEIEESTNSTGSKLWI 120
           FAVLEDKVE MLQ           QE DEVPLVRDPRK KG AQSE EESTNS GSKL I
Sbjct: 61  FAVLEDKVEGMLQRMTQVLRQYERQESDEVPLVRDPRKGKGPAQSETEESTNSAGSKLRI 120

Query: 121 G----------------------------------------LDKGKPTDQPESSEKRQNQ 168
           G                                        LDKGKP DQPESSEKR NQ
Sbjct: 121 GGNTRRRTQIFDSQKMKKQHKYSAPNGGRSDHDDRNSEPVSLDKGKPADQPESSEKRHNQ 180

[Close]

BLAST of Moc02g15300 vs. NCBI nr
Match: XP_022159109.1 (uncharacterized protein LOC111025548 [Momordica charantia])

HSP 1 Score: 185.7 bits (470), Expect = 4.7e-43
Identity = 109/201 (54.23%), Postives = 116/201 (57.71%), Query Frame = 0


Query: 109 IGLDKGKPTDQPESSEKRQNQKEKGFDLEELLDQADSPFTEEIMKEKVPPKFKLPTVKQF 168
           I L+KGKP D+PESSEKR NQKEKGFDLEELL QADSPFTEEIM+EKVPPKFKLPTVK F
Sbjct: 47  ISLNKGKPIDRPESSEKRHNQKEKGFDLEELLGQADSPFTEEIMREKVPPKFKLPTVKPF 106

Query: 169 DGTTDPVDHLDAYREWMDIYGVSEAV---------------------------------- 228
           DG T+PVDHLDAYREWMDIYGVS+A+                                  
Sbjct: 107 DGMTEPVDHLDAYREWMDIYGVSDAIRCRVFSTTLNGSARIWFRQLKRGSISSFKSLARA 166

Query: 229 -------------------------------------------EGLTDAVSLLAFMSGVR 233
                                                      EGLTDAVSLLAFMSGVR
Sbjct: 167 FMTQFVGGRCRSRPVAYLLTIKQRTAESLHDYVARFNEEKLQLEGLTDAVSLLAFMSGVR 226

[Close]

BLAST of Moc02g15300 vs. NCBI nr
Match: XP_022142767.1 (uncharacterized protein LOC111012805 [Momordica charantia])

HSP 1 Score: 134.0 bits (336), Expect = 1.6e-27
Identity = 79/124 (63.71%), Postives = 89/124 (71.77%), Query Frame = 0


Query: 109 IGLDKGKPTDQPESSEKRQNQKEKGFDLEELLDQADSPFTEEIMKEKVPPKFKLPTVKQF 168
           I LDKGKP D+PESSEKR NQK+KGFDLEELLDQADSPFTEEIM+EK   +     V +F
Sbjct: 63  ISLDKGKPADRPESSEKRHNQKKKGFDLEELLDQADSPFTEEIMREKRTTESLHDYVTRF 122

Query: 169 DGTTDPVDHLDAYREWMDIYGVSEAVEGLTDAVSLLAFMSGVRDEHLSFSLGKKTPSTFS 228
           +            +E +        VEGLTDAVSLLAF+ GVRDEHLSFS GK+TPSTFS
Sbjct: 123 N------------KEKLQ-------VEGLTDAVSLLAFIFGVRDEHLSFSFGKRTPSTFS 167

Query: 229 EALS 233
           EALS
Sbjct: 183 EALS 167

[Close]

BLAST of Moc02g15300 vs. NCBI nr
Match: XP_022150035.1 (uncharacterized protein LOC111018307 [Momordica charantia])

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-23
Identity = 76/161 (47.20%), Postives = 79/161 (49.07%), Query Frame = 0


Query: 149 EEIMKEKVPPKFKLPTVKQFDGTTDPVDHLDAYREWMDIYGVSEA--------------- 208
           EEIMK KVPPKFKLPTVKQFDGT D VDHLDAYREWMDIYGVSEA               
Sbjct: 2   EEIMKVKVPPKFKLPTVKQFDGTVDLVDHLDAYREWMDIYGVSEAVKCRVFSTTLSGSAR 61

Query: 209 ------------------------------------------------------------ 233
                                                                       
Sbjct: 62  VWFRQLKRGSISSFKSLAKAFVTQFIGGRSRSRPVAYLLTIKQRATESLHDYVARFNEEK 121

[Close]

BLAST of Moc02g15300 vs. ExPASy TrEMBL
Match: A0A6J1DWY0 (uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025293 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 1.0e-80
Identity = 192/349 (55.01%), Postives = 202/349 (57.88%), Query Frame = 0


Query: 1   MTPERSPRRSDDNYSTKRRLNLDDPQVGGSEDGTSQSNPERQEGLPEAHAITTLEPLQKQ 60
           MTPERSPRRSDD+ S KRRLNL DPQVGG EDGTSQ N ERQEGL E  A+TT EP QKQ
Sbjct: 1   MTPERSPRRSDDDCSAKRRLNLGDPQVGGPEDGTSQPNQERQEGLXEXXALTTPEPFQKQ 60

Query: 61  FAVLEDKVEDMLQQEPDEVPLVRDPRKRKGTAQSEIEESTNSTGSKLWIG---------- 120
           FAVLEDK       E DEVPLVRDP+K KG  +S+ EESTNS GSKL IG          
Sbjct: 61  FAVLEDK-------ESDEVPLVRDPKKGKGPTESDTEESTNSVGSKLRIGGNTRQRTRIF 120

Query: 121 ------------------------------LDKGKPTDQPESSEKRQNQKEKGFDLEELL 180
                                         LDKGKP D+PESSEKR + KEKGFDLEELL
Sbjct: 121 DPRKTKKQHKSPAPNGGKSDHDDRNSEPISLDKGKPADRPESSEKRHSHKEKGFDLEELL 180

Query: 181 DQADSPFTEEIMKEKVPPKFKLPTVKQFDGTTDPVDHLDAYREWMDIYGVSEA------- 233
           DQADSPFTEEIM+EKVPPKFKLPTVKQFD TTDPVDHLDAYREWMDIYGVSEA       
Sbjct: 181 DQADSPFTEEIMREKVPPKFKLPTVKQFDXTTDPVDHLDAYREWMDIYGVSEAVRCRVFS 240

[Close]

BLAST of Moc02g15300 vs. ExPASy TrEMBL
Match: A0A6J1CUA7 (uncharacterized protein LOC111014646 OS=Momordica charantia OX=3673 GN=LOC111014646 PE=4 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 1.2e-63
Identity = 150/218 (68.81%), Postives = 152/218 (69.72%), Query Frame = 0


Query: 1   MTPERSPRRSDDNYSTKRRLNLDDPQVGGSEDGTSQSNPERQEGLPEAHAITTLEPLQKQ 60
           MTPERSPRRSDDN S KRRLNLDDPQVGG EDGTSQ N ERQEGLPEAHA+TT EPLQKQ
Sbjct: 1   MTPERSPRRSDDNCSAKRRLNLDDPQVGGPEDGTSQPNLERQEGLPEAHALTTPEPLQKQ 60

Query: 61  FAVLEDKVEDMLQ-----------QEPDEVPLVRDPRKRKGTAQSEIEESTNSTGSKLWI 120
           FAVLEDKVE MLQ           QE DEVPLVRDPRK KG AQSE EESTNS GSKL I
Sbjct: 61  FAVLEDKVEGMLQRMTQVLRQYERQESDEVPLVRDPRKGKGPAQSETEESTNSAGSKLRI 120

Query: 121 G----------------------------------------LDKGKPTDQPESSEKRQNQ 168
           G                                        LDKGKP DQPESSEKR NQ
Sbjct: 121 GGNTRRRTQIFDSQKMKKQHKYSAPNGGRSDHDDRNSEPVSLDKGKPADQPESSEKRHNQ 180

[Close]

BLAST of Moc02g15300 vs. ExPASy TrEMBL
Match: A0A6J1E1E7 (uncharacterized protein LOC111025548 OS=Momordica charantia OX=3673 GN=LOC111025548 PE=4 SV=1)

HSP 1 Score: 185.7 bits (470), Expect = 2.3e-43
Identity = 109/201 (54.23%), Postives = 116/201 (57.71%), Query Frame = 0


Query: 109 IGLDKGKPTDQPESSEKRQNQKEKGFDLEELLDQADSPFTEEIMKEKVPPKFKLPTVKQF 168
           I L+KGKP D+PESSEKR NQKEKGFDLEELL QADSPFTEEIM+EKVPPKFKLPTVK F
Sbjct: 47  ISLNKGKPIDRPESSEKRHNQKEKGFDLEELLGQADSPFTEEIMREKVPPKFKLPTVKPF 106

Query: 169 DGTTDPVDHLDAYREWMDIYGVSEAV---------------------------------- 228
           DG T+PVDHLDAYREWMDIYGVS+A+                                  
Sbjct: 107 DGMTEPVDHLDAYREWMDIYGVSDAIRCRVFSTTLNGSARIWFRQLKRGSISSFKSLARA 166

Query: 229 -------------------------------------------EGLTDAVSLLAFMSGVR 233
                                                      EGLTDAVSLLAFMSGVR
Sbjct: 167 FMTQFVGGRCRSRPVAYLLTIKQRTAESLHDYVARFNEEKLQLEGLTDAVSLLAFMSGVR 226

[Close]

BLAST of Moc02g15300 vs. ExPASy TrEMBL
Match: A0A6J1CNT2 (uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012805 PE=4 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 7.8e-28
Identity = 79/124 (63.71%), Postives = 89/124 (71.77%), Query Frame = 0


Query: 109 IGLDKGKPTDQPESSEKRQNQKEKGFDLEELLDQADSPFTEEIMKEKVPPKFKLPTVKQF 168
           I LDKGKP D+PESSEKR NQK+KGFDLEELLDQADSPFTEEIM+EK   +     V +F
Sbjct: 63  ISLDKGKPADRPESSEKRHNQKKKGFDLEELLDQADSPFTEEIMREKRTTESLHDYVTRF 122

Query: 169 DGTTDPVDHLDAYREWMDIYGVSEAVEGLTDAVSLLAFMSGVRDEHLSFSLGKKTPSTFS 228
           +            +E +        VEGLTDAVSLLAF+ GVRDEHLSFS GK+TPSTFS
Sbjct: 123 N------------KEKLQ-------VEGLTDAVSLLAFIFGVRDEHLSFSFGKRTPSTFS 167

Query: 229 EALS 233
           EALS
Sbjct: 183 EALS 167

[Close]

BLAST of Moc02g15300 vs. ExPASy TrEMBL
Match: A0A6J1D7D2 (uncharacterized protein LOC111018307 OS=Momordica charantia OX=3673 GN=LOC111018307 PE=4 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 5.2e-24
Identity = 76/161 (47.20%), Postives = 79/161 (49.07%), Query Frame = 0


Query: 149 EEIMKEKVPPKFKLPTVKQFDGTTDPVDHLDAYREWMDIYGVSEA--------------- 208
           EEIMK KVPPKFKLPTVKQFDGT D VDHLDAYREWMDIYGVSEA               
Sbjct: 2   EEIMKVKVPPKFKLPTVKQFDGTVDLVDHLDAYREWMDIYGVSEAVKCRVFSTTLSGSAR 61

Query: 209 ------------------------------------------------------------ 233
                                                                       
Sbjct: 62  VWFRQLKRGSISSFKSLAKAFVTQFIGGRSRSRPVAYLLTIKQRATESLHDYVARFNEEK 121

The following BLAST results are available for this feature:

BLAST of Moc02g15300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158830.1	2.1e-80	55.01	uncharacterized protein LOC111025293 [Momordica charantia]	[more]
XP_022145129.1	2.4e-63	68.81	uncharacterized protein LOC111014646 [Momordica charantia]	[more]
XP_022159109.1	4.7e-43	54.23	uncharacterized protein LOC111025548 [Momordica charantia]	[more]
XP_022142767.1	1.6e-27	63.71	uncharacterized protein LOC111012805 [Momordica charantia]	[more]
XP_022150035.1	1.1e-23	47.20	uncharacterized protein LOC111018307 [Momordica charantia]	[more]

BLAST of Moc02g15300 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Moc02g15300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DWY0	1.0e-80	55.01	uncharacterized protein LOC111025293 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1CUA7	1.2e-63	68.81	uncharacterized protein LOC111014646 OS=Momordica charantia OX=3673 GN=LOC111014...	[more]
A0A6J1E1E7	2.3e-43	54.23	uncharacterized protein LOC111025548 OS=Momordica charantia OX=3673 GN=LOC111025...	[more]
A0A6J1CNT2	7.8e-28	63.71	uncharacterized protein LOC111012805 OS=Momordica charantia OX=3673 GN=LOC111012...	[more]
A0A6J1D7D2	5.2e-24	47.20	uncharacterized protein LOC111018307 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]

BLAST of Moc02g15300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 54..74
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..19
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 68..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 68..94
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 117..137
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 28..42
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..54

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g15300.1	Moc02g15300.1	mRNA