Homology
BLAST of Moc02g15190 vs. NCBI nr
Match:
XP_022157751.1 (probable glucan 1,3-alpha-glucosidase [Momordica charantia])
HSP 1 Score: 1907.1 bits (4939), Expect = 0.0e+00
Identity = 915/915 (100.00%), Postives = 915/915 (100.00%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD
Sbjct: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL
Sbjct: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM
Sbjct: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM
Sbjct: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK
Sbjct: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL
Sbjct: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNLSITDDWTV
Sbjct: 901 TIRKPNLSITDDWTV 915
BLAST of Moc02g15190 vs. NCBI nr
Match:
KAG6604702.1 (putative glucan 1,3-alpha-glucosidase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1783.8 bits (4619), Expect = 0.0e+00
Identity = 856/963 (88.89%), Postives = 898/963 (93.25%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
M +S+LLLLLLF LH TFV PWKK+EFR CNQTPFC+RARA KPGSCSLVAHDVSI+DGD
Sbjct: 1 MTVSYLLLLLLFLLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSISDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTA LLPRNQ D E+Q KPLLLALSVYQDGI+RLRIDEDPSL PPKKRF+VPDVIVDEF
Sbjct: 61 LTAILLPRNQ-DLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFY 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKK+WLQ I+TE IG+DL PSSIVYLSD YEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKIWLQRISTETIGNDLGPSSIVYLSDSYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVKEEGEDWEEKFRGHTD RPYGPQS+SFDVSFYD DFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDTDFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG PAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDEYDIPYDVLWLDIEHTDGKRYFTWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLA KGRRMVTIVDPHIKRDD+FPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
N YGYYFHMAT+EGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NAYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDEATFKNDEAFMVGS L
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEATFKNDEAFMVGSGL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK VSVYLPG+QSWYD RTGT Y+GG+THQLEVSEE IPAFQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQ AEGELY+DDGKSFEFKQGAYIHRRFVFS GKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ P S+S KFSSNCVIERIILLG+SG KSALVEP+N KVDIELGPLHFQTGRRISVL
Sbjct: 841 LNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV-MGVGIFLWLLMQDK-ETKKD---SKK--------GGSGGFGTHS 951
TIRKPNL ITDDWTV +G+GIFLWLL +++ +TKK SK+ G G G+ S
Sbjct: 901 TIRKPNLLITDDWTVTIGIGIFLWLLKENRGKTKKQEGYSKRRLPRIWHSGSGSGSGSGS 960
BLAST of Moc02g15190 vs. NCBI nr
Match:
XP_038900635.1 (probable glucan 1,3-alpha-glucosidase [Benincasa hispida])
HSP 1 Score: 1768.8 bits (4580), Expect = 0.0e+00
Identity = 837/915 (91.48%), Postives = 876/915 (95.74%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
MR+ +LLLL+L +LH TFV PWKK+EFRNCNQTPFC+RARA KPGSCSLVAHDVSINDGD
Sbjct: 1 MRVPYLLLLILLALHLTFVLPWKKEEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTAKLLPRNQ DP+H KPLLLALSVYQDGI+RLR+DEDPSLGP KKRF+VPDVI+DEFL
Sbjct: 61 LTAKLLPRNQ-DPDHPPKPLLLALSVYQDGILRLRVDEDPSLGPSKKRFQVPDVILDEFL 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
+KKLWLQ I+TE IGSDLSPSSIVYLSDGYEAVLR DPFEVFVREKSGKR+LSLNSHGLF
Sbjct: 121 SKKLWLQRISTETIGSDLSPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRILSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVKEEGEDWEEKFRGHTD RPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGK RGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKSRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQ+RIDT WMSEAGIVD+FFFVGPGPKDVVRQYTSVTG AMPQLF
Sbjct: 301 GSGWDAESGISLPSSQSRIDTLWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDE+DIPYDVLWLDIEHTDGKRYFTWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLAAKGR MVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGND+DGWCWPGSSSYLDM
Sbjct: 421 QRKLAAKGRHMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDYDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
NVYGYYF MATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NVYGYYFQMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIR+RYMLLPYFYTLFREANTSGIPVVRPLW+EFPSDE TFKNDEAFMVGSAL
Sbjct: 661 NTELMRDAIRVRYMLLPYFYTLFREANTSGIPVVRPLWLEFPSDEVTFKNDEAFMVGSAL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK+ SVYLPG+QSWYD RTG YRGG+THQLEVSEESIPAFQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKKASVYLPGKQSWYDFRTGATYRGGITHQLEVSEESIPAFQKAGTIIPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQAAEGELY+DDGKSFEFKQGA+IHRRFVFSGGKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSGGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ PI SSS KFSSNCVIERIILLG+S KSALVEP+NRKVDIELGPLHFQT R ISVL
Sbjct: 841 LNVGPIASSSTKFSSNCVIERIILLGHSRSKSALVEPENRKVDIELGPLHFQTARHISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL ITDDWTV
Sbjct: 901 TIRKPNLLITDDWTV 914
BLAST of Moc02g15190 vs. NCBI nr
Match:
XP_023532412.1 (probable glucan 1,3-alpha-glucosidase [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1767.3 bits (4576), Expect = 0.0e+00
Identity = 837/915 (91.48%), Postives = 872/915 (95.30%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
M +S+LLLLLLF LH TFV PWKK+EFR CNQTPFC+RARA KPGSCSLVAHDVSI DGD
Sbjct: 1 MTVSYLLLLLLFLLHLTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSITDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTA LLPRNQ D E+Q KPLLLALSVYQDGI+RLRIDEDPSL PPKKRF+VPDVIVDEF
Sbjct: 61 LTAILLPRNQ-DLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFY 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKK+WLQ I+TE IG+DLSPSSIVYLSD YEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKIWLQRISTETIGNDLSPSSIVYLSDDYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLR+KEEGEDWEEKFRGHTD RPYGPQS+SFDVSFYDADFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRIKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDADFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVD+FFFVGPGPKD+VRQYTSVTG PAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDIVRQYTSVTGTPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDEYDIPYDVLWLDIEHTDGKRYFTWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLA KGRRMVTIVDPHIKRDD+FPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
N YGYYFHMAT+EGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NAYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDEATFKNDEAFMVGS L
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEATFKNDEAFMVGSGL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK VSVYLPG+QSWYD RTGT Y+GG+THQLEVSEE IPAFQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQ AEGELY+DDGKSFEFKQGAYIHRRFVFS GKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ P S+S KFSSNCVIERIILLG+SG KSALVEP+N KVDIELGPLHFQTGRRISVL
Sbjct: 841 LNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL ITDDWTV
Sbjct: 901 TIRKPNLLITDDWTV 914
BLAST of Moc02g15190 vs. NCBI nr
Match:
XP_022947784.1 (probable glucan 1,3-alpha-glucosidase [Cucurbita moschata] >KAG7034833.1 putative glucan 1,3-alpha-glucosidase [Cucurbita argyrosperma subsp. argyrosperma])
HSP 1 Score: 1765.7 bits (4572), Expect = 0.0e+00
Identity = 837/915 (91.48%), Postives = 871/915 (95.19%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
M +S+LLLLLLF LH TFV PWKK+EFR CNQTPFC+RARA KPGSCSLVAHDVSI+DGD
Sbjct: 1 MTVSYLLLLLLFLLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSISDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTA LLPRNQ D E+Q KPLLLALSVYQDGI+RLRIDEDPSL PPKKRF+VPDVIVDEF
Sbjct: 61 LTAILLPRNQ-DLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFY 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKK+WLQ I+TE IG+DL PSSIVYLSD YEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKIWLQRISTETIGNDLGPSSIVYLSDSYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVKEEGEDWEEKFRGHTD RPYGPQS+SFDVSFYD DFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDTDFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG PAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDEYDIPYDVLWLDIEHTDGKRYFTWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLA KGRRMVTIVDPHIKRDD+FPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
N YGYYFHMAT+EGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NAYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDEATFKNDEAFMVGS L
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEATFKNDEAFMVGSGL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK VSVYLPG+QSWYD RTGT Y+GG+THQLEVSEE IPAFQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQ AEGELY+DDGKSFEFKQGAYIHRRFVFS GKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ P S+S KFSSNCVIERIILLG+SG KSALVEP+N KVDIELGPLHFQTGRRISVL
Sbjct: 841 LNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL ITDDWTV
Sbjct: 901 TIRKPNLLITDDWTV 914
BLAST of Moc02g15190 vs. ExPASy Swiss-Prot
Match:
Q9FN05 (Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE=1 SV=1)
HSP 1 Score: 1504.2 bits (3893), Expect = 0.0e+00
Identity = 708/920 (76.96%), Postives = 797/920 (86.63%), Query Frame = 0
Query: 4 SHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGDLTA 63
S L +L L S WKK+EFR+C+QTPFC+RAR+ PG+CSL+ DVSI DGDL A
Sbjct: 3 SLLFVLSLICFCSQTALSWKKEEFRSCDQTPFCKRARSRTPGACSLIVGDVSITDGDLVA 62
Query: 64 KLLPR--NQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFLT 123
KLLP+ NQ D + Q+KPL+L+LSVY+DGIVRL+IDED SL PPKKRF+VPDV+V EF
Sbjct: 63 KLLPKAPNQGDGD-QIKPLILSLSVYKDGIVRLKIDEDHSLNPPKKRFQVPDVVVSEFEE 122
Query: 124 KKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSG--KRVLSLNSHGL 183
KK+WLQ +ATE I D SPSS+VY+SDGYEAV+RHDPFEV+VREKSG +RV+SLNSHGL
Sbjct: 123 KKIWLQKVATETISGDTSPSSVVYVSDGYEAVVRHDPFEVYVREKSGDRRRVVSLNSHGL 182
Query: 184 FDFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPT 243
FDFEQL K EG++WEEKFR HTD RP GPQSISFDVSFYD+ FVYGIPE ATS ALKPT
Sbjct: 183 FDFEQLGRKTEGDNWEEKFRTHTDSRPSGPQSISFDVSFYDSSFVYGIPEHATSFALKPT 242
Query: 244 RGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDV 303
+GPGVE+SEPYRLFNLDVFEY HESPFGLYGSIP M+SHGK TSGFFWLNAAEMQIDV
Sbjct: 243 KGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVSHGKSGKTSGFFWLNAAEMQIDV 302
Query: 304 LGSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQL 363
L +GWDAESGISLPSS +RIDTFWMSEAGIVD+FFFVGP PKDVV+QY SVTG AMPQL
Sbjct: 303 LANGWDAESGISLPSSHSRIDTFWMSEAGIVDTFFFVGPEPKDVVKQYASVTGTSAMPQL 362
Query: 364 FATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVE 423
FAT YHQCRWNY+DEEDV QVDSKFDE+DIPYDVLWLDIEHTDGKRYFTWD LFP+P E
Sbjct: 363 FATGYHQCRWNYKDEEDVAQVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDSVLFPHPEE 422
Query: 424 MQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLD 483
MQ+KLAAKGR+MVTIVDPHIKRDDS+ LHKEA++ GYYVKD++G DFDGWCWPGSSSY+D
Sbjct: 423 MQKKLAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYID 482
Query: 484 MLSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHREL 543
MLSPEIR WWG +FS +NYVGSTPSLY WNDMNEPSVFNGPEVTMPRDALH GGVEHRE+
Sbjct: 483 MLSPEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNGPEVTMPRDALHVGGVEHREV 542
Query: 544 HNVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVP 603
HN YGYYFHMAT++GLV R +GKDRPFVLSRA+F GTQRYGA+WTGDNTA+W+ LRVS+P
Sbjct: 543 HNAYGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIP 602
Query: 604 MIVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGE 663
MI+TLGLTG++FSGAD+GGFFGNPE ELLVRWYQ+GA+YPFFRGHAHHDTKRREPWLFGE
Sbjct: 603 MILTLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGE 662
Query: 664 RNTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSA 723
RNTELMRDAI RY LLPYFYTLFREAN +G+PVVRPLWMEFP DEATF NDEAFMVGS
Sbjct: 663 RNTELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSG 722
Query: 724 LLVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPR 783
LLVQG+YTK + SVYLPG++SWYDLR G Y GG TH+++ EESIPAFQKAGTI+PR
Sbjct: 723 LLVQGVYTKGTTQASVYLPGKESWYDLRNGKTYVGGKTHKMDAPEESIPAFQKAGTIIPR 782
Query: 784 KDRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLT 843
KDRFRRSS+QM NDPYTLVVALN+SQ AEGELY+DDGKSFEF++G+YIHRRFVFS G LT
Sbjct: 783 KDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRRGSYIHRRFVFSKGVLT 842
Query: 844 SLNMAPIGSSSAKFSSNCVIERIILLGY-SGPKSALVEPDNRKVDIELGPLH---FQTGR 903
S N+AP A+ SS C+I+RIILLG+ SGPKSALVEP N+K +IE+GPL
Sbjct: 843 STNLAP---PEARLSSQCLIDRIILLGHSSGPKSALVEPLNQKAEIEMGPLRMGGLVASS 902
Query: 904 RISVLTIRKPNLSITDDWTV 916
VLTIRKP + + DWTV
Sbjct: 903 GTKVLTIRKPGVRVDQDWTV 918
BLAST of Moc02g15190 vs. ExPASy Swiss-Prot
Match:
B9F676 (Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os03g0216600 PE=3 SV=1)
HSP 1 Score: 1317.0 bits (3407), Expect = 0.0e+00
Identity = 633/924 (68.51%), Postives = 740/924 (80.09%), Query Frame = 0
Query: 2 RISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGS----CSLVAHDVSI- 61
R++ LLLLLL S S WKKDEFRNCNQTPFC+RAR P S SL A +++
Sbjct: 11 RVAVLLLLLLAS--SPAARAWKKDEFRNCNQTPFCKRARTRAPHSLDAPLSLDAASLAVA 70
Query: 62 NDGDLTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLG-PPKKRFEVPDVI 121
DG LTA L +L+PLLL LS +RL+IDED S PP +RF+VPDV+
Sbjct: 71 TDGSLTASL------SHPSRLRPLLLRLSALPPHALRLQIDEDYSSNTPPHRRFQVPDVL 130
Query: 122 VDEFLTKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREK-SGKRVLSL 181
+ + + L L T G S LS + V++HDPFE+ VR SG VLS
Sbjct: 131 LPDVEARTLHLPQPKTSAAG-----VSTFALSSDVDVVVKHDPFELTVRRAGSGAPVLSF 190
Query: 182 NSHGLFDFEQLR-VKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPER-AT 241
NSHGLFDFE L+ K+EGE WEE+FR HTD RP GPQSI+FDVSFY ADFVYG+PE +T
Sbjct: 191 NSHGLFDFEPLQESKQEGETWEEQFRSHTDTRPRGPQSITFDVSFYGADFVYGLPEHGST 250
Query: 242 SLALKPTRGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNA 301
SLAL+PTRGPG E+SEPYRLFNLDVFEY+HESPFGLYGSIP MI+HG +SGFFWLNA
Sbjct: 251 SLALRPTRGPGAEESEPYRLFNLDVFEYLHESPFGLYGSIPFMIAHGD-GPSSGFFWLNA 310
Query: 302 AEMQIDVLGSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTG 361
AEMQIDVL GWD S + RIDT WM+EAG+VD+FFFVG PKDV++QY SVTG
Sbjct: 311 AEMQIDVLAPGWDGASS----TENGRIDTLWMAEAGVVDAFFFVGSEPKDVIKQYISVTG 370
Query: 362 APAMPQLFATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKA 421
P+MPQ FA AYHQCRWNYRDEEDV VDS FDE+DIPYDVLWLDIEHTDGKRYFTWD +
Sbjct: 371 TPSMPQQFAVAYHQCRWNYRDEEDVAGVDSGFDEHDIPYDVLWLDIEHTDGKRYFTWDHS 430
Query: 422 LFPNPVEMQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWP 481
FPNP MQ K+A KGR+MVTIVDPHIKRD SF LH+EA+ KGYYVKDA G DFDGWCWP
Sbjct: 431 AFPNPEVMQGKIADKGRKMVTIVDPHIKRDSSFHLHEEATAKGYYVKDATGKDFDGWCWP 490
Query: 482 GSSSYLDMLSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQG 541
G+SSY DML+PEIR WW +KFS ENY GSTP+LYIWNDMNEPSVFNGPEVTMPRDA+H G
Sbjct: 491 GASSYPDMLNPEIREWWADKFSYENYKGSTPTLYIWNDMNEPSVFNGPEVTMPRDAVHYG 550
Query: 542 GVEHRELHNVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWD 601
VEHRELHN YGYYFHMATA+GL+KRG+GKDRPFVLSRA FAG+QRYGA+WTGDN+ADWD
Sbjct: 551 DVEHRELHNAYGYYFHMATADGLLKRGEGKDRPFVLSRAFFAGSQRYGAIWTGDNSADWD 610
Query: 602 QLRVSVPMIVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRR 661
L+ S+PM++TLGLTG++FSGAD+GGFFGNPE +LLVRWYQ+GAFYPFFRGHAHHDTKRR
Sbjct: 611 HLKSSIPMVLTLGLTGMTFSGADIGGFFGNPEPDLLVRWYQVGAFYPFFRGHAHHDTKRR 670
Query: 662 EPWLFGERNTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDE 721
EPWLFGER T LMR+AI +RY LLPY+YTLFREA+ +G+PV+RPLW+EFP D+ T+ N E
Sbjct: 671 EPWLFGERRTALMREAIHMRYSLLPYYYTLFREASVTGVPVMRPLWLEFPDDKETYNNGE 730
Query: 722 AFMVGSALLVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQK 781
AFMVG +LL QGIY + K VSVYLPGE+ WYDLR G+PY+GGV+H+LEVSE+SIP+FQ+
Sbjct: 731 AFMVGPSLLAQGIYEEGQKSVSVYLPGEELWYDLRNGSPYKGGVSHKLEVSEDSIPSFQR 790
Query: 782 AGTILPRKDRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFV 841
AG I+PRKDRFRRSSTQMVNDPYTLV+ALN+S AAEGELYVDDGKS++++QGA+IHRRFV
Sbjct: 791 AGAIVPRKDRFRRSSTQMVNDPYTLVIALNSSSAAEGELYVDDGKSYDYQQGAFIHRRFV 850
Query: 842 FSGGKLTSLNMAPIGSSSAKFSSNCVIERIILLGY-SGPKSALVEPDNRKVDIELGPLHF 901
F+ KLTS+N+AP + KFS+ CVIERII+LG SG K A+VEP N +VDIELGP+
Sbjct: 851 FADNKLTSMNIAPKNLGNKKFSTECVIERIIILGVSSGSKKAIVEPGNHEVDIELGPISL 910
Query: 902 QTGRRISVLTIRKPNLSITDDWTV 916
++G T+RKPN+ + DDWT+
Sbjct: 911 RSGSSSVAPTVRKPNVRVVDDWTI 916
BLAST of Moc02g15190 vs. ExPASy Swiss-Prot
Match:
Q94502 (Neutral alpha-glucosidase AB OS=Dictyostelium discoideum OX=44689 GN=modA PE=3 SV=1)
HSP 1 Score: 832.4 bits (2149), Expect = 5.3e-240
Identity = 420/960 (43.75%), Postives = 602/960 (62.71%), Query Frame = 0
Query: 6 LLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRAR-ALKPGSCSLVAHDVSINDGDLTAK 65
++L ++ SL + +F+ C + FC+R R + + G + + + N + + K
Sbjct: 8 IILSIVCSLFIGSIESVDTSKFKTCKDSHFCKRNRVSHEVGVMNEMKSKQNFNIVEGSIK 67
Query: 66 LLPRNQ----DDPEHQLKPLLLA--LSVYQDGIVRLRIDE-DPSLGPPKKRFEVPDVIVD 125
L+ + D E K LL L +Y+ GIVR+R E +P L K+R++V DV++D
Sbjct: 68 LVKQENTIYFDLQEQNQKSNLLTMKLEIYEGGIVRMRAQEKEPLLN--KQRYQVQDVLLD 127
Query: 126 EFLTKKL-WLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNS 185
T + W Q + + + +++ PF++ V + + ++ NS
Sbjct: 128 TIKTVPIQWKQEPSKQSNTFSFKHGE----KECCYVLVQLVPFKLDVYIMN-ELAITTNS 187
Query: 186 HGLFDFEQL------------RVKEEGED-------------------------WEEKFR 245
LF FE + + +EE ++ WEE+F
Sbjct: 188 DNLFHFEPISDKPQPLPPKEKKSEEENKEANQEEDNNNNNNDNNEEQQVSTEGYWEERFG 247
Query: 246 GHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTRGPGVEDSEPYRLFNLDVFE 305
H D +P GP SI D +F + VYGIPE T L+LK T G G+ + +PYRL+NLDVFE
Sbjct: 248 SHQDSKPNGPMSIGMDFTFVGSSHVYGIPEHTTRLSLKSTTGNGINE-QPYRLYNLDVFE 307
Query: 306 YIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVLGSGWDAESGISLPSSQNRI 365
Y + LYG +PLMISH + T G FWLNAAE +D+ ++ P S ++
Sbjct: 308 YEIDKTMALYGHVPLMISH-DTKKTVGVFWLNAAETFVDI--------EDVTTPVSPSK- 367
Query: 366 DTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQ 425
T W+SE+GI+D F+ GP P + +QY +TG A+PQ+F+ YHQC+WNY+ E+DV+Q
Sbjct: 368 KTHWISESGIIDVFYLTGPTPSTIFKQYAYLTGTTALPQMFSLGYHQCKWNYKSEDDVKQ 427
Query: 426 VDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEMQRKLAAKGRRMVTIVDPHI 485
VD+ FDE IPYDV+WLDIEHTDGKRYFTWD FP P +MQ + AK R+MVTIVDPHI
Sbjct: 428 VDNGFDENHIPYDVIWLDIEHTDGKRYFTWDNNNFPTPADMQNIIGAKHRKMVTIVDPHI 487
Query: 486 KRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGEKFSLENYV 545
KRD+++ +H EA+ KGYY+K+ GND+DGWCWPGSSSYLD +PEIR WW +F + Y
Sbjct: 488 KRDNNYYVHSEATSKGYYIKNKDGNDYDGWCWPGSSSYLDFTNPEIRKWWATQFGYDKYK 547
Query: 546 GSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELHNVYGYYFHMATAEGLVKR- 605
GSTP+LYIWNDMNEPSVFNGPEV+M +DA H GG EHR++HN+YGYY+HMA+A+GLV+R
Sbjct: 548 GSTPNLYIWNDMNEPSVFNGPEVSMHKDAKHHGGFEHRDVHNLYGYYYHMASADGLVQRN 607
Query: 606 GDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGG 665
D DRPFVLSRA +AG+QR GA+WTGDN+A W L +S PM++++ L G++FSGADVGG
Sbjct: 608 ADQNDRPFVLSRAFYAGSQRIGAIWTGDNSAQWSHLEISNPMLLSMNLAGITFSGADVGG 667
Query: 666 FFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPY 725
FFGNP+ ELL RWYQ GAF PFFRGHAH D++RREPWLF E T ++R+AI RY LP
Sbjct: 668 FFGNPDAELLTRWYQAGAFQPFFRGHAHLDSRRREPWLFNEPYTTIIREAIVKRYSYLPL 727
Query: 726 FYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSALLVQGIYTKEAKEVSVYLP 785
+YT F + +G PV+RPLW+++P + F D+ +++G +LLV+ + + K + V LP
Sbjct: 728 WYTTFYQNTLNGAPVMRPLWVQYPKEANLFDVDDHYLIGDSLLVKPVTQQSCKTMKVLLP 787
Query: 786 GE---QSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRKDRFRRSSTQMVNDPY 845
G+ + WYD+ T P GV +++ E IP +Q+ G+I+ +K+R RRS+ QM +DPY
Sbjct: 788 GQSVNEIWYDVDTEKPINAGVI-EIDTPLEKIPVYQRGGSIISKKERVRRSTYQMRDDPY 847
Query: 846 TLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTSLNMAPIGSSSAKFSS 905
T+ +AL++S++A+G+LY+DD SF++K+G +++R+F F L+ + + SS +
Sbjct: 848 TIRIALDSSKSAQGQLYIDDEHSFDYKKGKFLYRQFTFKDNVLSFSDAS--NKSSTSYKP 907
Query: 906 NCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVLTIRKPNLSITDDWTV 916
N IE+I++LG P S ++ L F+ +S LTIRKP+L + D+ +
Sbjct: 908 NVTIEKIVILGVQKPHSITCNITGKE------KLSFEYDSTLSKLTIRKPDLLVDTDFII 940
BLAST of Moc02g15190 vs. ExPASy Swiss-Prot
Match:
Q8BHN3 (Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1)
HSP 1 Score: 830.1 bits (2143), Expect = 2.6e-239
Identity = 425/947 (44.88%), Postives = 578/947 (61.03%), Query Frame = 0
Query: 24 KDEFRNCNQTPFCQRARALKPGSCSLVA--HDVSINDGDLTAKLLPRNQDDPEHQLKPLL 83
+ F+ C+++ FC+R R+++PG A + + LT L+ H++ +L
Sbjct: 35 RSNFKTCDESSFCKRQRSIRPGLSPYRALLDTLQLGPDALTVHLI--------HEVTKVL 94
Query: 84 LALSV--YQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFLTKKLWLQSIATEQIGSDLS 143
L L + Q + R+RIDE L P + R+ VPDV+V + T +L + G D +
Sbjct: 95 LVLELQGLQKNMTRIRIDE---LEPRRPRYRVPDVLVADPPTARLSVS-------GRDDN 154
Query: 144 PSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLFDFEQLRV------------ 203
+ Y+ +L PF + + E +LS+N+ GL FE R
Sbjct: 155 SVELTVAEGPYKIILTAQPFRLDLLE-DRSLLLSVNARGLMAFEHQRAPRVPQESKDPAE 214
Query: 204 ------------------------KEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADF 263
K+E WEE F+ H+D +PYGP S+ D S +
Sbjct: 215 GNGAQPEATPGDGDKPEETQEKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEH 274
Query: 264 VYGIPERATSLALKPTRGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRG 323
VYGIPE A SL LK T G EPYRL+NLDVF+Y +P LYGS+P++++H R
Sbjct: 275 VYGIPEHADSLRLKVTEG-----GEPYRLYNLDVFQYELNNPMALYGSVPVLLAHSFHRD 334
Query: 324 TSGFFWLNAAEMQIDV---------LGSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFF 383
G FWLNAAE +D+ G D G + D WMSE+GI+D F
Sbjct: 335 L-GIFWLNAAETWVDISSNTAGKTLFGKMLDYLQG---SGETPQTDIRWMSESGIIDVFL 394
Query: 384 FVGPGPKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVL 443
+GP DV RQY S+TG A+P LF+ YHQ RWNYRDE DV +VD FD++++P DV+
Sbjct: 395 MLGPSVFDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNMPCDVI 454
Query: 444 WLDIEHTDGKRYFTWDKALFPNPVEMQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKK 503
WLDIEH DGKRYFTWD FP P+ M LA+K R++V IVDPHIK D + +H+E
Sbjct: 455 WLDIEHADGKRYFTWDPTRFPQPLNMLEHLASKRRKLVAIVDPHIKVDSGYRVHEELRNH 514
Query: 504 GYYVKDAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEP 563
G YVK G+D++GWCWPGS+SY D +P +R+WW FS +NY GS P+LY+WNDMNEP
Sbjct: 515 GLYVKTRDGSDYEGWCWPGSASYPDFTNPRMRAWWSNMFSFDNYEGSAPNLYVWNDMNEP 574
Query: 564 SVFNGPEVTMPRDALHQGGVEHRELHNVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFA 623
SVFNGPEVTM +DA+H GG EHR++HN+YG Y HMATA+GL++R G +RPFVLSRA F+
Sbjct: 575 SVFNGPEVTMLKDAVHYGGWEHRDIHNIYGLYVHMATADGLIQRSGGIERPFVLSRAFFS 634
Query: 624 GTQRYGAVWTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQL 683
G+QR+GAVWTGDNTA+WD L++S+PM ++L L GLSF GADVGGFF NPE ELLVRWYQ+
Sbjct: 635 GSQRFGAVWTGDNTAEWDHLKISIPMCLSLALVGLSFCGADVGGFFKNPEPELLVRWYQM 694
Query: 684 GAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPYFYTLFREANTSGIPVV 743
GA+ PFFR HAH DT RREPWL + + +RDA+ RY LLP++YTLF +A+ G PV+
Sbjct: 695 GAYQPFFRAHAHLDTGRREPWLLASQYQDAIRDALFQRYSLLPFWYTLFYQAHKEGFPVM 754
Query: 744 RPLWMEFPSDEATFKNDEAFMVGSALLVQGIYTKEAKEVSVYLPG-EQSWYDLRTGTPYR 803
RPLW+++P D +TF ++ FM+G ALL+ + A V VYLPG E+ WYD+++ +
Sbjct: 755 RPLWVQYPEDMSTFSIEDQFMLGDALLIHPVSDAGAHGVQVYLPGQEEVWYDIQSYQKHH 814
Query: 804 GGVTHQLEVSEESIPAFQKAGTILPRKDRFRRSSTQMVNDPYTLVVALNNSQAAEGELYV 863
G T L V+ SIP FQ+ GTI+PR R RRSS M +DP TL VAL+ A+GEL++
Sbjct: 815 GPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKDDPITLFVALSPQGTAQGELFL 874
Query: 864 DDGKSFEFK-QGAYIHRRFVFSGGKLTSLNMAPIGSSSAKFSSNCVIERIILLGYSGPKS 916
DDG +F ++ + ++ RRF FSG L S + P G + IER++++G P +
Sbjct: 875 DDGHTFNYQTRHEFLLRRFSFSGSTLVSSSADPKG----HLETPIWIERVVIMGAGKPAA 934
BLAST of Moc02g15190 vs. ExPASy Swiss-Prot
Match:
P79403 (Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1)
HSP 1 Score: 828.9 bits (2140), Expect = 5.8e-239
Identity = 426/945 (45.08%), Postives = 575/945 (60.85%), Query Frame = 0
Query: 24 KDEFRNCNQTPFCQRARALKPGSCSLVA--HDVSINDGDLTAKLLPRNQDDPEHQLKPLL 83
+ F+ C ++ FC+R R+++PG A + + LT L+ E L+
Sbjct: 35 RSNFKTCEESSFCKRQRSIRPGQSPYRALLDSLQLGPDTLTIHLI------NEVTKVLLV 94
Query: 84 LALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFLTKKLWLQSIATEQIGSDLSPS 143
L L Q + R+RIDE L P + R+ VPDV+V E T +L + G D +
Sbjct: 95 LELQGLQKNMTRIRIDE---LEPRRPRYRVPDVLVAEPPTARLSVS-------GQDDNSV 154
Query: 144 SIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLFDFEQLRV-------------- 203
+ Y+ +L PF + + E +LS+N+ GL +FE R
Sbjct: 155 EVTVAEGPYKIILTARPFRLDLLE-DRSLLLSVNARGLLNFEHQRAPRVSQGSKDPAEGD 214
Query: 204 ----------------------KEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVY 263
K+E WEE F+ H+D +PYGP S+ D S + VY
Sbjct: 215 GAQPEEAPGDGDKPEEIQGKAEKDEPGAWEETFKTHSDSKPYGPTSVGLDFSLPGMEHVY 274
Query: 264 GIPERATSLALKPTRGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTS 323
GIPE A SL LK T G +PYRL+NLDVF+Y +P LYGS+P++++H R
Sbjct: 275 GIPEHADSLRLKVTEG-----GDPYRLYNLDVFQYELYNPMALYGSVPVLLAHSPHRDL- 334
Query: 324 GFFWLNAAEMQIDV---------LGSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFV 383
G FWLNAAE +D+ G D G + D WMSE+GI+D F +
Sbjct: 335 GIFWLNAAETWVDISSNTAGKTLFGKMLDYLQG---SGETPQTDVRWMSESGIIDVFLLL 394
Query: 384 GPGPKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWL 443
GP DV RQY S+TG A+P LF+ YHQ RWNYRDE DV +V+ FD++++P D +WL
Sbjct: 395 GPSVFDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVNQGFDDHNLPCDFIWL 454
Query: 444 DIEHTDGKRYFTWDKALFPNPVEMQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGY 503
DIEH DGKRYFTWD + FP P M LA+K R++V IVDPHIK D S+ +H+E G
Sbjct: 455 DIEHADGKRYFTWDPSRFPQPRTMLEHLASKRRKLVAIVDPHIKVDSSYRVHEELQNLGL 514
Query: 504 YVKDAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSV 563
YVK G+D++GWCWPG++SY D +P++R+WW + F ENY GS+ +LY+WNDMNEPSV
Sbjct: 515 YVKTRDGSDYEGWCWPGAASYPDFTNPKMRAWWADMFRFENYEGSSSNLYVWNDMNEPSV 574
Query: 564 FNGPEVTMPRDALHQGGVEHRELHNVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGT 623
FNGPEVTM +DA H GG EHR+LHN+YG+Y HMATA+GLV R G +RPFVLSRA FAG+
Sbjct: 575 FNGPEVTMLKDAQHYGGWEHRDLHNIYGFYVHMATADGLVLRSGGVERPFVLSRAFFAGS 634
Query: 624 QRYGAVWTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGA 683
QR+GAVWTGDNTA+WD L++S+PM ++LGL G+SF GADVGGFF NPE ELLVRWYQ+GA
Sbjct: 635 QRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGVSFCGADVGGFFKNPEPELLVRWYQMGA 694
Query: 684 FYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRP 743
+ PFFR HAH DT RREPWL + +++RDA+ RY LLP++YTLF +A+ G+PV+R
Sbjct: 695 YQPFFRAHAHLDTGRREPWLLPTQYQDMIRDALGQRYSLLPFWYTLFYQAHREGVPVMRA 754
Query: 744 LWMEFPSDEATFKNDEAFMVGSALLVQGIYTKEAKEVSVYLPGE-QSWYDLRTGTPYRGG 803
LW+ +P D TF D+ F++G ALLV + EA V VYLPG+ + WYD+ + Y G
Sbjct: 755 LWVHYPQDVTTFSIDDEFLLGDALLVHPVTDSEAHGVQVYLPGQGEVWYDVHSYQKYHGP 814
Query: 804 VTHQLEVSEESIPAFQKAGTILPRKDRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDD 863
T L V+ SIP FQ+ GTI+PR R RRSS M +DP TL VAL+ A+GEL++DD
Sbjct: 815 QTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSDCMKDDPITLFVALSPQGTAQGELFLDD 874
Query: 864 GKSFEFKQG-AYIHRRFVFSGGKLTSLNMAPIGSSSAKFSSNCVIERIILLGYSGPKSAL 916
G +F ++ G ++ RRF FSG L S + S F + IER++++G P + +
Sbjct: 875 GHTFNYQTGHEFLLRRFSFSGNTLVSSS----ADSKGHFETPVWIERVVIIGAGKPATVV 934
BLAST of Moc02g15190 vs. ExPASy TrEMBL
Match:
A0A6J1DZ48 (probable glucan 1,3-alpha-glucosidase OS=Momordica charantia OX=3673 GN=LOC111024385 PE=3 SV=1)
HSP 1 Score: 1907.1 bits (4939), Expect = 0.0e+00
Identity = 915/915 (100.00%), Postives = 915/915 (100.00%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD
Sbjct: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL
Sbjct: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM
Sbjct: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM
Sbjct: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK
Sbjct: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL
Sbjct: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNLSITDDWTV
Sbjct: 901 TIRKPNLSITDDWTV 915
BLAST of Moc02g15190 vs. ExPASy TrEMBL
Match:
A0A6J1G7X2 (probable glucan 1,3-alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111451543 PE=3 SV=1)
HSP 1 Score: 1765.7 bits (4572), Expect = 0.0e+00
Identity = 837/915 (91.48%), Postives = 871/915 (95.19%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
M +S+LLLLLLF LH TFV PWKK+EFR CNQTPFC+RARA KPGSCSLVAHDVSI+DGD
Sbjct: 1 MTVSYLLLLLLFLLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDVSISDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTA LLPRNQ D E+Q KPLLLALSVYQDGI+RLRIDEDPSL PPKKRF+VPDVIVDEF
Sbjct: 61 LTAILLPRNQ-DLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFY 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKK+WLQ I+TE IG+DL PSSIVYLSD YEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKIWLQRISTETIGNDLGPSSIVYLSDSYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVKEEGEDWEEKFRGHTD RPYGPQS+SFDVSFYD DFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRVKEEGEDWEEKFRGHTDTRPYGPQSVSFDVSFYDTDFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG PAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDEYDIPYDVLWLDIEHTDGKRYFTWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDRALFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLA KGRRMVTIVDPHIKRDD+FPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
N YGYYFHMAT+EGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NAYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREANTSG+PVVRPLWMEFPSDEATFKNDEAFMVGS L
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGVPVVRPLWMEFPSDEATFKNDEAFMVGSGL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK VSVYLPG+QSWYD RTGT Y+GG+THQLEVSEE IPAFQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQ AEGELY+DDGKSFEFKQGAYIHRRFVFS GKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ P S+S KFSSNCVIERIILLG+SG KSALVEP+N KVDIELGPLHFQTGRRISVL
Sbjct: 841 LNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL ITDDWTV
Sbjct: 901 TIRKPNLLITDDWTV 914
BLAST of Moc02g15190 vs. ExPASy TrEMBL
Match:
A0A6J1I0F6 (probable glucan 1,3-alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC111469732 PE=3 SV=1)
HSP 1 Score: 1763.8 bits (4567), Expect = 0.0e+00
Identity = 836/915 (91.37%), Postives = 871/915 (95.19%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
M +S+LLLLLLF LH TFV PWKK+EFR CNQTPFC+RARA KPGSCSLVAHD SI DGD
Sbjct: 1 MTVSYLLLLLLFVLHFTFVLPWKKEEFRTCNQTPFCKRARAFKPGSCSLVAHDASITDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTA LLPRNQ D E+Q KPLLLALSVYQDGI+RLRIDEDPSL PPKKRF+VPDVIVDEF
Sbjct: 61 LTAILLPRNQ-DLENQSKPLLLALSVYQDGILRLRIDEDPSLAPPKKRFQVPDVIVDEFY 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
TKK+WLQ I+TE IG+DLSPSSIVYLSDGYEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 TKKIWLQRISTETIGNDLSPSSIVYLSDGYEAVLRKDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLR+KEEGEDWEEKFRGHTD RPYGPQSISFDVSFYDADFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRIKEEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+HESPFG+YGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYVHESPFGIYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQNRIDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG PAMPQLF
Sbjct: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTPAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDEYDIPYDVLWLDIEHTD KRYFTWD+ALFPNP +M
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEYDIPYDVLWLDIEHTDAKRYFTWDRALFPNPEDM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
QRKLA KGRRMVTIVDPHIKRDD+FPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM
Sbjct: 421 QRKLATKGRRMVTIVDPHIKRDDNFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPEIRSWWGEKFSLENY GSTPSLYIWNDMNEPSVFNGPEVTMPR+ALHQGGVEHRELH
Sbjct: 481 LSPEIRSWWGEKFSLENYAGSTPSLYIWNDMNEPSVFNGPEVTMPRNALHQGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
NVYGYYFHMAT+EGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDN+ADWD LRVSVPM
Sbjct: 541 NVYGYYFHMATSEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNSADWDHLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPETELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIRIRYMLLPYFYTLFREAN+SG+PVVRPLWMEFPSDEATFKNDEAFMVGS L
Sbjct: 661 NTELMRDAIRIRYMLLPYFYTLFREANSSGVPVVRPLWMEFPSDEATFKNDEAFMVGSGL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK VSVYLPG+QSWYD RTGT Y+GG+THQLEVSEE IPAFQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKRVSVYLPGKQSWYDFRTGTAYKGGITHQLEVSEEGIPAFQKAGTIMPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQ AEGELY+DDGKSFEFKQGAYIHRRFVFS GKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQEAEGELYIDDGKSFEFKQGAYIHRRFVFSAGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ P S+S KFSSNCVIERIILLG+SG KSALVEP+N KVDIELGPLHFQTGRRISVL
Sbjct: 841 LNVRPNASASTKFSSNCVIERIILLGHSGSKSALVEPENIKVDIELGPLHFQTGRRISVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL ITDDWTV
Sbjct: 901 TIRKPNLLITDDWTV 914
BLAST of Moc02g15190 vs. ExPASy TrEMBL
Match:
A0A1S3CCU7 (probable glucan 1,3-alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103499585 PE=3 SV=1)
HSP 1 Score: 1718.7 bits (4450), Expect = 0.0e+00
Identity = 811/915 (88.63%), Postives = 857/915 (93.66%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
MR +LLLLLL + H TFV PWKKDEFRNCNQTPFC+RARA KPGSCSLVAHDVSINDGD
Sbjct: 1 MRAPYLLLLLLLASHLTFVLPWKKDEFRNCNQTPFCKRARAFKPGSCSLVAHDVSINDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTAKLLPRNQ DP+H KPLLLALSVYQDGIVRLRIDEDPSLGPPKKRF++PDVIVDEFL
Sbjct: 61 LTAKLLPRNQ-DPDHPSKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFQLPDVIVDEFL 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
+KKLWLQ I+TE IGSDL PSSIVYLSD YEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 SKKLWLQRISTETIGSDLRPSSIVYLSDDYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVK EGEDWEEKFRGHTD RPYGPQSISFDVSFYDADFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRVKNEGEDWEEKFRGHTDTRPYGPQSISFDVSFYDADFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+++SPFGLYGSIP MISHGKLRGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYLNDSPFGLYGSIPFMISHGKLRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQ+ IDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG AMPQLF
Sbjct: 301 GSGWDAESGISLPSSQSSIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDE+DIPYDVLWLDI+HTDGKRY TWD+ALFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIDHTDGKRYMTWDRALFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Q+KLAAKGRRMVT+VDPH+KR+DSF LHKEASK GYYVKDAAGND+DGWCWPGSSSYLD
Sbjct: 421 QKKLAAKGRRMVTVVDPHVKREDSFTLHKEASKNGYYVKDAAGNDYDGWCWPGSSSYLDA 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPE+RSWWGEKFS +NYVGSTPSLYIWNDMNEPSVF+GPE TMPR+ALH GGVEHRELH
Sbjct: 481 LSPEVRSWWGEKFSFQNYVGSTPSLYIWNDMNEPSVFSGPEGTMPRNALHHGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
N YGYYFHMATAEGLVKRGDG DRPFVLSRA FAGTQ+YG VWTGD++ADWD LRVSVPM
Sbjct: 541 NAYGYYFHMATAEGLVKRGDGNDRPFVLSRAAFAGTQKYGTVWTGDSSADWDFLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTG+SFSGADVGGFFGNPE ELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGVSFSGADVGGFFGNPETELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIR+RYMLLPYFYTLFREANTSGIPV+RPLWMEFPSDE TFKNDEAFMVGSAL
Sbjct: 661 NTELMRDAIRVRYMLLPYFYTLFREANTSGIPVIRPLWMEFPSDEVTFKNDEAFMVGSAL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK+VSVY PG+QSWYD RTGT Y+GGVTHQ+EV EE IP FQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKKVSVYFPGKQSWYDFRTGTIYKGGVTHQIEVFEEGIPTFQKAGTIIPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DRFRRSSTQMVNDPYTLVVALN+SQ AEGELY+DDGKSFEFKQGA+IHRRFVFS GKLTS
Sbjct: 781 DRFRRSSTQMVNDPYTLVVALNSSQTAEGELYIDDGKSFEFKQGAFIHRRFVFSDGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ PI SSS KFSSNC IERIILLG+SG KSALVEP+NRKVDIELGPLHF GRR SVL
Sbjct: 841 LNVGPIASSSTKFSSNCFIERIILLGHSGAKSALVEPENRKVDIELGPLHFLKGRRTSVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL I DDWTV
Sbjct: 901 TIRKPNLLIRDDWTV 914
BLAST of Moc02g15190 vs. ExPASy TrEMBL
Match:
A0A0A0LJZ9 (Gal_mutarotas_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G338840 PE=3 SV=1)
HSP 1 Score: 1705.6 bits (4416), Expect = 0.0e+00
Identity = 805/915 (87.98%), Postives = 858/915 (93.77%), Query Frame = 0
Query: 1 MRISHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGD 60
MR +LLLLLL SLH T V PWKKDEFRNCNQTPFC+RARA K GSCSLVAHDVSINDGD
Sbjct: 1 MRAPYLLLLLLLSLHLTLVLPWKKDEFRNCNQTPFCKRARAFKRGSCSLVAHDVSINDGD 60
Query: 61 LTAKLLPRNQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFL 120
LTAKLLPRNQ DP+H PLLL LSVYQDGIVRLRIDEDPSLGPPKKRF++P+VIVDEFL
Sbjct: 61 LTAKLLPRNQ-DPDHPPNPLLLVLSVYQDGIVRLRIDEDPSLGPPKKRFQLPNVIVDEFL 120
Query: 121 TKKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSGKRVLSLNSHGLF 180
++KLWLQ I+TE IGSDL PSSIVYLSDGYEAVLR DPFEVFVREKSGKRVLSLNSHGLF
Sbjct: 121 SQKLWLQRISTETIGSDLRPSSIVYLSDGYEAVLRQDPFEVFVREKSGKRVLSLNSHGLF 180
Query: 181 DFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPTR 240
DFEQLRVK+EGEDWEEKFRGHTD RP+GPQSISFDVSFYDADFVYGIPE ATSLALKPTR
Sbjct: 181 DFEQLRVKDEGEDWEEKFRGHTDTRPFGPQSISFDVSFYDADFVYGIPEHATSLALKPTR 240
Query: 241 GPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDVL 300
GP VE+SEPYRLFNLDVFEY+H+SPFGLYGSIP MISHGK RGTSGFFWLNAAEMQIDVL
Sbjct: 241 GPDVEESEPYRLFNLDVFEYLHDSPFGLYGSIPFMISHGKSRGTSGFFWLNAAEMQIDVL 300
Query: 301 GSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLF 360
GSGWDAESGISLPSSQ+ IDTFWMSEAGIVD+FFFVGPGPKDVVRQYTSVTG AMPQLF
Sbjct: 301 GSGWDAESGISLPSSQSSIDTFWMSEAGIVDTFFFVGPGPKDVVRQYTSVTGTSAMPQLF 360
Query: 361 ATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVEM 420
ATAYHQCRWNYRDEEDV VDSKFDE+DIPYDVLWLDI+HTDGKRY TWD++LFPNP EM
Sbjct: 361 ATAYHQCRWNYRDEEDVAHVDSKFDEHDIPYDVLWLDIDHTDGKRYMTWDRSLFPNPEEM 420
Query: 421 QRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDM 480
Q+KLAAKGR MVT+VDPH+KR+DSF LHKEASKKGYYVKDAAGND+DGWCWPGSSSYLD
Sbjct: 421 QKKLAAKGRYMVTVVDPHVKREDSFTLHKEASKKGYYVKDAAGNDYDGWCWPGSSSYLDA 480
Query: 481 LSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHRELH 540
LSPE+RSWWGEKFSL+NYVGSTP+LYIWNDMNEPSVF+GPE TMPR+ALH GGVEHRELH
Sbjct: 481 LSPEVRSWWGEKFSLQNYVGSTPTLYIWNDMNEPSVFSGPEGTMPRNALHYGGVEHRELH 540
Query: 541 NVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPM 600
N YGYYFHMAT+EGLVKRGDG DRPFVLSRA FAGTQ+YG VWTGD++A+WD LRVSVPM
Sbjct: 541 NAYGYYFHMATSEGLVKRGDGNDRPFVLSRAAFAGTQKYGTVWTGDSSAEWDYLRVSVPM 600
Query: 601 IVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGER 660
++TLGLTGLSFSGADVGGFFGNPE ELLVRW+QLGAFYPFFRGHAHHDTKRREPWLFGER
Sbjct: 601 VLTLGLTGLSFSGADVGGFFGNPEAELLVRWFQLGAFYPFFRGHAHHDTKRREPWLFGER 660
Query: 661 NTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSAL 720
NTELMRDAIR+RY+LLPYFYTLFREAN +GIPVVRPLWMEFPSDE TFKNDEAFMVGSAL
Sbjct: 661 NTELMRDAIRVRYVLLPYFYTLFREANMTGIPVVRPLWMEFPSDEVTFKNDEAFMVGSAL 720
Query: 721 LVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRK 780
LVQGIYTKEAK+VSVYLPG+QSWYD RTGT Y+GG+THQLEV EESIP FQKAGTI+PRK
Sbjct: 721 LVQGIYTKEAKKVSVYLPGKQSWYDFRTGTIYKGGITHQLEVFEESIPTFQKAGTIIPRK 780
Query: 781 DRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTS 840
DR RRSSTQMVNDPYTLVVALN+SQAAEGELY+DDGKSFEFKQGA+IHRRFVFS GKLTS
Sbjct: 781 DRSRRSSTQMVNDPYTLVVALNSSQAAEGELYIDDGKSFEFKQGAFIHRRFVFSDGKLTS 840
Query: 841 LNMAPIGSSSAKFSSNCVIERIILLGYSGPKSALVEPDNRKVDIELGPLHFQTGRRISVL 900
LN+ PI SSS KFSSNCVIERIILLG+SG KSALVEP+NRKVDIELGPLHF GR SVL
Sbjct: 841 LNVGPIASSSTKFSSNCVIERIILLGHSGAKSALVEPENRKVDIELGPLHFLRGRGSSVL 900
Query: 901 TIRKPNLSITDDWTV 916
TIRKPNL I+DDWTV
Sbjct: 901 TIRKPNLLISDDWTV 914
BLAST of Moc02g15190 vs. TAIR 10
Match:
AT5G63840.1 (Glycosyl hydrolases family 31 protein )
HSP 1 Score: 1504.2 bits (3893), Expect = 0.0e+00
Identity = 708/920 (76.96%), Postives = 797/920 (86.63%), Query Frame = 0
Query: 4 SHLLLLLLFSLHSTFVFPWKKDEFRNCNQTPFCQRARALKPGSCSLVAHDVSINDGDLTA 63
S L +L L S WKK+EFR+C+QTPFC+RAR+ PG+CSL+ DVSI DGDL A
Sbjct: 3 SLLFVLSLICFCSQTALSWKKEEFRSCDQTPFCKRARSRTPGACSLIVGDVSITDGDLVA 62
Query: 64 KLLPR--NQDDPEHQLKPLLLALSVYQDGIVRLRIDEDPSLGPPKKRFEVPDVIVDEFLT 123
KLLP+ NQ D + Q+KPL+L+LSVY+DGIVRL+IDED SL PPKKRF+VPDV+V EF
Sbjct: 63 KLLPKAPNQGDGD-QIKPLILSLSVYKDGIVRLKIDEDHSLNPPKKRFQVPDVVVSEFEE 122
Query: 124 KKLWLQSIATEQIGSDLSPSSIVYLSDGYEAVLRHDPFEVFVREKSG--KRVLSLNSHGL 183
KK+WLQ +ATE I D SPSS+VY+SDGYEAV+RHDPFEV+VREKSG +RV+SLNSHGL
Sbjct: 123 KKIWLQKVATETISGDTSPSSVVYVSDGYEAVVRHDPFEVYVREKSGDRRRVVSLNSHGL 182
Query: 184 FDFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYDADFVYGIPERATSLALKPT 243
FDFEQL K EG++WEEKFR HTD RP GPQSISFDVSFYD+ FVYGIPE ATS ALKPT
Sbjct: 183 FDFEQLGRKTEGDNWEEKFRTHTDSRPSGPQSISFDVSFYDSSFVYGIPEHATSFALKPT 242
Query: 244 RGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGKLRGTSGFFWLNAAEMQIDV 303
+GPGVE+SEPYRLFNLDVFEY HESPFGLYGSIP M+SHGK TSGFFWLNAAEMQIDV
Sbjct: 243 KGPGVEESEPYRLFNLDVFEYDHESPFGLYGSIPFMVSHGKSGKTSGFFWLNAAEMQIDV 302
Query: 304 LGSGWDAESGISLPSSQNRIDTFWMSEAGIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQL 363
L +GWDAESGISLPSS +RIDTFWMSEAGIVD+FFFVGP PKDVV+QY SVTG AMPQL
Sbjct: 303 LANGWDAESGISLPSSHSRIDTFWMSEAGIVDTFFFVGPEPKDVVKQYASVTGTSAMPQL 362
Query: 364 FATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIEHTDGKRYFTWDKALFPNPVE 423
FAT YHQCRWNY+DEEDV QVDSKFDE+DIPYDVLWLDIEHTDGKRYFTWD LFP+P E
Sbjct: 363 FATGYHQCRWNYKDEEDVAQVDSKFDEHDIPYDVLWLDIEHTDGKRYFTWDSVLFPHPEE 422
Query: 424 MQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLD 483
MQ+KLAAKGR+MVTIVDPHIKRDDS+ LHKEA++ GYYVKD++G DFDGWCWPGSSSY+D
Sbjct: 423 MQKKLAAKGRKMVTIVDPHIKRDDSYFLHKEATQMGYYVKDSSGKDFDGWCWPGSSSYID 482
Query: 484 MLSPEIRSWWGEKFSLENYVGSTPSLYIWNDMNEPSVFNGPEVTMPRDALHQGGVEHREL 543
MLSPEIR WWG +FS +NYVGSTPSLY WNDMNEPSVFNGPEVTMPRDALH GGVEHRE+
Sbjct: 483 MLSPEIRKWWGGRFSYKNYVGSTPSLYTWNDMNEPSVFNGPEVTMPRDALHVGGVEHREV 542
Query: 544 HNVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVP 603
HN YGYYFHMAT++GLV R +GKDRPFVLSRA+F GTQRYGA+WTGDNTA+W+ LRVS+P
Sbjct: 543 HNAYGYYFHMATSDGLVMREEGKDRPFVLSRAIFPGTQRYGAIWTGDNTAEWEHLRVSIP 602
Query: 604 MIVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGE 663
MI+TLGLTG++FSGAD+GGFFGNPE ELLVRWYQ+GA+YPFFRGHAHHDTKRREPWLFGE
Sbjct: 603 MILTLGLTGITFSGADIGGFFGNPEPELLVRWYQVGAYYPFFRGHAHHDTKRREPWLFGE 662
Query: 664 RNTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSA 723
RNTELMRDAI RY LLPYFYTLFREAN +G+PVVRPLWMEFP DEATF NDEAFMVGS
Sbjct: 663 RNTELMRDAIHTRYTLLPYFYTLFREANVTGVPVVRPLWMEFPQDEATFSNDEAFMVGSG 722
Query: 724 LLVQGIYTKEAKEVSVYLPGEQSWYDLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPR 783
LLVQG+YTK + SVYLPG++SWYDLR G Y GG TH+++ EESIPAFQKAGTI+PR
Sbjct: 723 LLVQGVYTKGTTQASVYLPGKESWYDLRNGKTYVGGKTHKMDAPEESIPAFQKAGTIIPR 782
Query: 784 KDRFRRSSTQMVNDPYTLVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLT 843
KDRFRRSS+QM NDPYTLVVALN+SQ AEGELY+DDGKSFEF++G+YIHRRFVFS G LT
Sbjct: 783 KDRFRRSSSQMDNDPYTLVVALNSSQEAEGELYIDDGKSFEFRRGSYIHRRFVFSKGVLT 842
Query: 844 SLNMAPIGSSSAKFSSNCVIERIILLGY-SGPKSALVEPDNRKVDIELGPLH---FQTGR 903
S N+AP A+ SS C+I+RIILLG+ SGPKSALVEP N+K +IE+GPL
Sbjct: 843 STNLAP---PEARLSSQCLIDRIILLGHSSGPKSALVEPLNQKAEIEMGPLRMGGLVASS 902
Query: 904 RISVLTIRKPNLSITDDWTV 916
VLTIRKP + + DWTV
Sbjct: 903 GTKVLTIRKPGVRVDQDWTV 918
BLAST of Moc02g15190 vs. TAIR 10
Match:
AT3G23640.1 (heteroglycan glucosidase 1 )
HSP 1 Score: 336.3 bits (861), Expect = 8.4e-92
Identity = 178/505 (35.25%), Postives = 272/505 (53.86%), Query Frame = 0
Query: 340 PKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIE 399
P V+ + G MP +A YHQCRW+Y ++ V ++ F + IP DV+W+DI+
Sbjct: 178 PTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDID 237
Query: 400 HTDGKRYFTWDKALFPNPVEMQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVK 459
+ DG R FT+DK FP+P + + L + G + + ++DP IK+++ + ++ SK ++
Sbjct: 238 YMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWIS 297
Query: 460 DAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGE--KFSLENYVGSTPSLYIWNDMNEPSVF 519
A G F G WPG + D + + RSWW K + N V IWNDMNEP+VF
Sbjct: 298 RADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDG-----IWNDMNEPAVF 357
Query: 520 NGPEVTMPRDALHQ-----GGVE-HRELHNVYGYYFHMATAEGLVKRGDGKDRPFVLSRA 579
TMP + +H GGV+ H HNVYG +T EG+ + D RPFVL+RA
Sbjct: 358 KVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGM-ELADKNKRPFVLTRA 417
Query: 580 LFAGTQRYGAVWTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGGFFGNPEIELLVRW 639
F G+QRY A WTGDN ++W+ L +S+ M++ LGL+G SG D+GGF GN L RW
Sbjct: 418 GFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRW 477
Query: 640 YQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPYFYTLFREANTSGI 699
+GA +PF RGH+ T EPW FGE E+ R A++ RY LLP+FYTLF A+T+G
Sbjct: 478 MGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGA 537
Query: 700 PVVRPLWMEFPSDEATFKNDEAFMVGSALLVQGIYTKE-AKEVSVYLPGEQSWYDLRTGT 759
PV P++ P D + F++G L+ + + + E+ LP
Sbjct: 538 PVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILP------------ 597
Query: 760 PYRGGVTHQLEV--SEESIPA-FQKAGTILPRKDRFRRSSTQMVNDPYTLVVALNNSQAA 819
G+ H+ + S +P + + G+I+ ++D TL+V+L+ + A
Sbjct: 598 ---RGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKA 657
Query: 820 EGELYVDDGKSFEFKQGAYIHRRFV 833
+G L+ DDG + + +G ++ ++
Sbjct: 658 KGLLFEDDGDGYGYTKGRFLVTHYI 661
BLAST of Moc02g15190 vs. TAIR 10
Match:
AT3G23640.2 (heteroglycan glucosidase 1 )
HSP 1 Score: 336.3 bits (861), Expect = 8.4e-92
Identity = 178/505 (35.25%), Postives = 272/505 (53.86%), Query Frame = 0
Query: 340 PKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDVLWLDIE 399
P V+ + G MP +A YHQCRW+Y ++ V ++ F + IP DV+W+DI+
Sbjct: 178 PTAVLESLSHAIGTVFMPPKWALGYHQCRWSYMSDKRVAEIAQTFRDKKIPSDVIWMDID 237
Query: 400 HTDGKRYFTWDKALFPNPVEMQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEASKKGYYVK 459
+ DG R FT+DK FP+P + + L + G + + ++DP IK+++ + ++ SK ++
Sbjct: 238 YMDGFRCFTFDKERFPDPSALAKDLHSNGFKAIWMLDPGIKQEEGYYVYDSGSKNDVWIS 297
Query: 460 DAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGE--KFSLENYVGSTPSLYIWNDMNEPSVF 519
A G F G WPG + D + + RSWW K + N V IWNDMNEP+VF
Sbjct: 298 RADGKPFTGEVWPGPCVFPDYTNSKARSWWANLVKEFVSNGVDG-----IWNDMNEPAVF 357
Query: 520 NGPEVTMPRDALHQ-----GGVE-HRELHNVYGYYFHMATAEGLVKRGDGKDRPFVLSRA 579
TMP + +H GGV+ H HNVYG +T EG+ + D RPFVL+RA
Sbjct: 358 KVVTKTMPENNIHHGDDELGGVQNHSHYHNVYGMLMARSTYEGM-ELADKNKRPFVLTRA 417
Query: 580 LFAGTQRYGAVWTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGGFFGNPEIELLVRW 639
F G+QRY A WTGDN ++W+ L +S+ M++ LGL+G SG D+GGF GN L RW
Sbjct: 418 GFIGSQRYAATWTGDNLSNWEHLHMSISMVLQLGLSGQPLSGPDIGGFAGNATPRLFGRW 477
Query: 640 YQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPYFYTLFREANTSGI 699
+GA +PF RGH+ T EPW FGE E+ R A++ RY LLP+FYTLF A+T+G
Sbjct: 478 MGVGAMFPFCRGHSEAGTDDHEPWSFGEECEEVCRAALKRRYQLLPHFYTLFYIAHTTGA 537
Query: 700 PVVRPLWMEFPSDEATFKNDEAFMVGSALLVQGIYTKE-AKEVSVYLPGEQSWYDLRTGT 759
PV P++ P D + F++G L+ + + + E+ LP
Sbjct: 538 PVAAPIFFADPIDSRLRAVENGFLLGPLLIYASTLSSQGSHELQHILP------------ 597
Query: 760 PYRGGVTHQLEV--SEESIPA-FQKAGTILPRKDRFRRSSTQMVNDPYTLVVALNNSQAA 819
G+ H+ + S +P + + G+I+ ++D TL+V+L+ + A
Sbjct: 598 ---RGIWHRFDFADSHPDLPTLYLQGGSIISLAPPHLHVGEFSLSDDLTLLVSLDENGKA 657
Query: 820 EGELYVDDGKSFEFKQGAYIHRRFV 833
+G L+ DDG + + +G ++ ++
Sbjct: 658 KGLLFEDDGDGYGYTKGRFLVTHYI 661
BLAST of Moc02g15190 vs. TAIR 10
Match:
AT3G45940.1 (Glycosyl hydrolases family 31 protein )
HSP 1 Score: 303.9 bits (777), Expect = 4.6e-82
Identity = 225/745 (30.20%), Postives = 346/745 (46.44%), Query Frame = 0
Query: 161 VFVREKSGKRVLSLNSHGLFDFEQLRVKEEGE------DWEEKFRGHTDKRPYGPQSISF 220
V V+E SG ++ + + F F +R + GE +E F K Y S S
Sbjct: 110 VTVQEISGPELILIFTVDPFSF-AVRRRSNGETIFNTSSSDESFGEMVFKDQYLEISTSL 169
Query: 221 --DVSFYDADFVYGIPERATSLALKPTRGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSI 280
D S Y +G +A + L P +EPY LF DV + + LYGS
Sbjct: 170 PKDASLYG----FGENSQANGIKLVP--------NEPYTLFTEDVSAFNLNT--DLYGSH 229
Query: 281 PLMISHGKLRGTSGFFWLNAAEMQIDVLGSGWDAESGISLPSSQNRIDTFWMSEA----- 340
P+ + LR SG + ++ L + + +D F+ ++
Sbjct: 230 PVYMD---LRNVSGKAYAHSV------------------LLLNSHGMDVFYRGDSLTYKV 289
Query: 341 --GIVDSFFFVGPGPKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQVDSKFD 400
G+ D +FF GP P +VV QYTS+ G PA ++ +HQCRW YR+ V+ V +
Sbjct: 290 IGGVFDFYFFAGPSPLNVVDQYTSLIGRPAPMPYWSLGFHQCRWGYRNVSVVKDVVDNYQ 349
Query: 401 EYDIPYDVLWLDIEHTDGKRYFTWDKALFPNP--VEMQRKLAAKGRRMVTIVDPHIKRDD 460
+ IP DV+W D ++ DG + FT D FP+ + ++ G + V I DP I +
Sbjct: 350 KAKIPLDVIWNDADYMDGYKDFTLDLVNFPHAKLLSFLDRIHKMGMKYVVIKDPGIGVNA 409
Query: 461 SFPLHKEASKKGYYVKDAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGEKFSLENYVGSTP 520
S+ +++ ++K G F WPG + D L+P+ SWWG++ + + P
Sbjct: 410 SYGVYQRGMASDVFIK-YEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDE--IRRFHELVP 469
Query: 521 SLYIWNDMNEPSVFNGPE----VTMPRDALHQGGVEHRELHNVYGYYFHMATAEGLVKRG 580
+W DMNE + T+P A H GV + H++YG+ +AT + L+
Sbjct: 470 IDGLWIDMNEINATGHKASLGFKTIPTSAYHYNGVREYDAHSIYGFSEAIATHKALL-AV 529
Query: 581 DGKDRPFVLSRALFAGTQRYGAVWTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGGF 640
GK RPF+LSR+ F G+ +Y A WTGDN W L+VS+ ++ G+ G+ G+D+ GF
Sbjct: 530 QGK-RPFILSRSTFVGSGQYAAHWTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGF 589
Query: 641 FGNPEIELLVRWYQLGAFYPFFRGHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPYF 700
F EL RW ++GAFYPF R HA + R+E + +G E R+A+ +RY LLP+
Sbjct: 590 FPPTPEELCNRWIEVGAFYPFSRDHADYYAPRKELYQWG-TVAESARNALGMRYKLLPFL 649
Query: 701 YTLFREANTSGIPVVRPLWMEFPSDEATFKNDEAFMVGSALLVQGIYTKEAKEVSVYLPG 760
YTL EA+ SG P+ RPL+ FP + + F++GS+L++ + + +V P
Sbjct: 650 YTLNYEAHMSGAPIARPLFFSFPEFTECYGLSKQFLLGSSLMISPVLEQGKTQVEALFP- 709
Query: 761 EQSWY---DLRTGTPYRGGVTHQLEVSEESIPAFQKAGTILPRKDRFRRSSTQMVNDPYT 820
SWY D+ + G L + ILP + Q+V P
Sbjct: 710 PGSWYHMFDMTQVVVSKNGRLFTLPAPFNVVNVHLYQNAILPMQ--------QVVAFP-- 769
Query: 821 LVVALNNSQAAEGELYVDDGKSFEFKQGAYIHRRFVFSGGKLTSLNM-APIGSSSAK--- 870
A + A G+L++DD + E K G GK T ++ A +G+ S K
Sbjct: 770 ---AGASEGYASGKLFLDDDELPEMKLG----------NGKSTYIDFYASVGNESVKIWS 788
BLAST of Moc02g15190 vs. TAIR 10
Match:
AT1G68560.1 (alpha-xylosidase 1 )
HSP 1 Score: 293.5 bits (750), Expect = 6.2e-79
Identity = 214/775 (27.61%), Postives = 337/775 (43.48%), Query Frame = 0
Query: 161 VFVREKSGKRVLSLNSHGLFDFEQLRVKEEGEDWEEKFRGHTDKRPYGPQSISFDVSFYD 220
+ V+E SG ++ + F F VK E T + Q + S
Sbjct: 119 ITVQEISGSELIFSYTTDPFTF---AVKRRSN--HETLFNTTSSLVFKDQYLEISTSLPK 178
Query: 221 ADFVYGIPERATSLALKPTRGPGVEDSEPYRLFNLDVFEYIHESPFGLYGSIPLMISHGK 280
+YG+ E + + +K + +EPY L+ DV + LYGS P
Sbjct: 179 EASLYGLGENSQANGIK------LVPNEPYTLYTEDVSAINLNT--DLYGSHP------- 238
Query: 281 LRGTSGFFWLNAAEMQIDVLGSGWDAESGISLPSSQNRIDTFWMSEA-------GIVDSF 340
M +D+ G A + L + N +D F+ ++ G+ D +
Sbjct: 239 --------------MYMDLRNVGGKAYAHAVLLLNSNGMDVFYRGDSLTYKVIGGVFDFY 298
Query: 341 FFVGPGPKDVVRQYTSVTGAPAMPQLFATAYHQCRWNYRDEEDVEQVDSKFDEYDIPYDV 400
F GP P +VV QYT + G PA ++ +HQCRW Y + VE V + + IP DV
Sbjct: 299 FIAGPSPLNVVDQYTQLIGRPAPMPYWSLGFHQCRWGYHNLSVVEDVVDNYKKAKIPLDV 358
Query: 401 LWLDIEHTDGKRYFTWDKALFPNP--VEMQRKLAAKGRRMVTIVDPHIKRDDSFPLHKEA 460
+W D +H DG + FT + +P + K+ G + + I DP I + S+ + A
Sbjct: 359 IWNDDDHMDGHKDFTLNPVAYPRAKLLAFLDKIHKIGMKYIVINDPGIGVNASYGTFQRA 418
Query: 461 SKKGYYVKDAAGNDFDGWCWPGSSSYLDMLSPEIRSWWGEKFSLENYVGSTPSLYIWNDM 520
++K G F WPG + D L+P+ SWWG++ ++ + P +W DM
Sbjct: 419 MAADVFIK-YEGKPFLAQVWPGPVYFPDFLNPKTVSWWGDE--IKRFHDLVPIDGLWIDM 478
Query: 521 NEPSVFNGPEVTMPR--------------------------------------------- 580
NE S F T+P
Sbjct: 479 NEVSNFCSGLCTIPEGKQCPSGEGPGWVCCLDCKNITKTRWDDPPYKINATGVVAPVGFK 538
Query: 581 ----DALHQGGVEHRELHNVYGYYFHMATAEGLVKRGDGKDRPFVLSRALFAGTQRYGAV 640
A H GV + H++YG+ +AT +GL+ GK RPF+LSR+ F G+ +Y A
Sbjct: 539 TIATSATHYNGVREYDAHSIYGFSETIATHKGLL-NVQGK-RPFILSRSTFVGSGQYAAH 598
Query: 641 WTGDNTADWDQLRVSVPMIVTLGLTGLSFSGADVGGFFGNPEIELLVRWYQLGAFYPFFR 700
WTGDN W L+VS+ ++ G+ G+ G+D+ GF+ P EL RW ++GAFYPF R
Sbjct: 599 WTGDNQGTWQSLQVSISTMLNFGIFGVPMVGSDICGFYPQPTEELCNRWIEVGAFYPFSR 658
Query: 701 GHAHHDTKRREPWLFGERNTELMRDAIRIRYMLLPYFYTLFREANTSGIPVVRPLWMEFP 760
HA++ + R+E + + + + R+A+ +RY +LP+ YTL EA+ +G P+ RPL+ FP
Sbjct: 659 DHANYYSPRQELYQW-DTVADSARNALGMRYKILPFLYTLNYEAHMTGAPIARPLFFSFP 718
Query: 761 SDEATFKNDEAFMVGSALLVQGIYTKEAKEVSVYLPGEQSWY---DLRTGTPYRGGVTHQ 820
+ N F++GS+ ++ + + EV P SWY D+ + G
Sbjct: 719 EYTECYGNSRQFLLGSSFMISPVLEQGKTEVEALFP-PGSWYHMFDMTQAVVSKNGKRVT 778
Query: 821 LEVSEESIPAFQKAGTILPRKDRFRRSSTQMVNDPYTLVVALNNSQA---AEGELYVDDG 870
L + TILP + S P++LV+A + A G+LY+D+
Sbjct: 779 LPAPLNFVNVHLYQNTILPTQQGGLISKDARTT-PFSLVIAFPAGASEGYATGKLYLDED 838
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
XP_022157751.1 | 0.0e+00 | 100.00 | probable glucan 1,3-alpha-glucosidase [Momordica charantia] | [more] |
KAG6604702.1 | 0.0e+00 | 88.89 | putative glucan 1,3-alpha-glucosidase, partial [Cucurbita argyrosperma subsp. so... | [more] |
XP_038900635.1 | 0.0e+00 | 91.48 | probable glucan 1,3-alpha-glucosidase [Benincasa hispida] | [more] |
XP_023532412.1 | 0.0e+00 | 91.48 | probable glucan 1,3-alpha-glucosidase [Cucurbita pepo subsp. pepo] | [more] |
XP_022947784.1 | 0.0e+00 | 91.48 | probable glucan 1,3-alpha-glucosidase [Cucurbita moschata] >KAG7034833.1 putativ... | [more] |
Match Name | E-value | Identity | Description | |
Q9FN05 | 0.0e+00 | 76.96 | Probable glucan 1,3-alpha-glucosidase OS=Arabidopsis thaliana OX=3702 GN=PSL5 PE... | [more] |
B9F676 | 0.0e+00 | 68.51 | Probable glucan 1,3-alpha-glucosidase OS=Oryza sativa subsp. japonica OX=39947 G... | [more] |
Q94502 | 5.3e-240 | 43.75 | Neutral alpha-glucosidase AB OS=Dictyostelium discoideum OX=44689 GN=modA PE=3 S... | [more] |
Q8BHN3 | 2.6e-239 | 44.88 | Neutral alpha-glucosidase AB OS=Mus musculus OX=10090 GN=Ganab PE=1 SV=1 | [more] |
P79403 | 5.8e-239 | 45.08 | Neutral alpha-glucosidase AB OS=Sus scrofa OX=9823 GN=GANAB PE=1 SV=1 | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1DZ48 | 0.0e+00 | 100.00 | probable glucan 1,3-alpha-glucosidase OS=Momordica charantia OX=3673 GN=LOC11102... | [more] |
A0A6J1G7X2 | 0.0e+00 | 91.48 | probable glucan 1,3-alpha-glucosidase OS=Cucurbita moschata OX=3662 GN=LOC111451... | [more] |
A0A6J1I0F6 | 0.0e+00 | 91.37 | probable glucan 1,3-alpha-glucosidase OS=Cucurbita maxima OX=3661 GN=LOC11146973... | [more] |
A0A1S3CCU7 | 0.0e+00 | 88.63 | probable glucan 1,3-alpha-glucosidase OS=Cucumis melo OX=3656 GN=LOC103499585 PE... | [more] |
A0A0A0LJZ9 | 0.0e+00 | 87.98 | Gal_mutarotas_2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G33... | [more] |