Moc02g15030 (gene) Bitter gourd (OHB3-1) v2

Overview
NameMoc02g15030
Typegene
OrganismMomordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
DescriptionReverse transcriptase
Locationchr2: 11158396 .. 11160156 (+)
RNA-Seq ExpressionMoc02g15030
SyntenyMoc02g15030
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAGATTCTGGTGCCACCCACAATTTCATGACAGAAACCGAAGCACGTCGGCTGAACTTGCGATGGGATAAAGACCTAGGAAAGATGAAAGCGGTCAACTCGGCGGCCCTCTCCATCATGGGAGTTGCAAAAAAAGTTTCAGTAAAGCTAGGGACATGGAGTGGACAGGTCGACTTTATGATAGTGCAGATGGACGACTTCGACAAAGTATTGGAGATGGAATTTCTATTGGAACACAAAGTCATCCCCATGCACCTCGTTAAGTGCCTGGTTGTAACGGGTTCCCATCCCACAATTGTTCAGACAAGCATCAAACAACCAAGCGGAGCGAAGATGATATCAGCTCTCCAGCTAAATAAAGGTCTCGCTCGTGACGAGCCGACCTTCATGGCCATTCTTGTTGTGGAAGGCGGAAATTCTGAAGAGCCAGCTCCTAGAGAAATCCAACGAGTCTTGGAGGTGTATGCCGATGTAATGCCAGACAACCTGCCCAAAAATCTACCTCCAAGACGGGGGATAGACCATGAGATTGAATTATTACCGGGGGTAAAACCGCCCACCAAGAACGCCTATCGGATGGCTCCTCCCGAGCTAGCCGAACTGAGGAAACAACTTGATGAACTACTGAATGCTGGATTCATCCGCCCGACTAAGGCTCCCTATAGGGCCCCAGTCCTTTTTCAGAAGAAGAAAGACGGAAGTCTCCGCCTATGCATCGACTATCGGGCATTGAACAAACTCACAGTCCGCAACAAGTACCCTTTGCCCATCATCACAGACCTGTTCGATCAACTTCATGAGGCAAAGTACTTCTCAAAGCTCGATCTGCGATCTGGATACTATCAGCTGCGTATCGCAGAAGGAGATGAACCGAAGACGACCGGCATCACTCGGTATGAGGCCTTTGAATTTCTTGTCATGCCCTTCGGTCTTACGAATGCTCCCGCCACCTTCTGTACCTTAATGAACCAAGTGTTTCACGAATATCTCGACAAATTCGTGGTGGTTTACCTAGATGATATAGTCGTCTACAATCCAACCATGGAAGAACACCAGGTACATTTACAGCTTGTTTTCGAGAAGCTCAAACAGAACCAGTTGTATGTTAAATGGGAAAAGTGTTCCTTTGCCCAAGAACGCATCAGTTTCTTGGGTCATGTGATAGAATATGGTCGAATAGGCATGGAGGATGGAAAGGTCAAAGCCATTTAAGAGTGGAAGATTCCGACGTCCATCACAGAACTACGTTCATTCCTCGGATTAGCCAATTACTATAGGCGATTCGTGGAAGGATTCTCGAAACAGACCGAGCCCTTAACAGAATTACTGAAGAAGAACCAGAAGTGGAACTGGACCCCAGAATGTCACGCCGCATTTGAGAGTTTGAAAAAGGTGATGATGGAGGGGCCGGTGTTGGGAATTGCAGACGTCACAAGACCGTTCGAAGTCGAGACTAATGCGTCAGACTTCGCCCTGGGAGGAGTGCTTCTCCAAGACGGTCACCCCATTGCATACGAGAGTCGGAAGTTGAATGATGCTGAAAGGAGGTATGCCGCCTCCAAGAAAGAGATGTTAGCAGTAGTAGTCTACTGCTTGAGGGCCTGGAGGCAATATCTCCTAGGGGCCAAGTTCGTTGTCAAGACCGATAACAGCTCAGTCTGTCACTTCTTCAACCAACCGAAGTTGTCGTCCAAGCAAGCTAGGTGGCAAGAGTACCTTGCCGAGTTCGATTTTCAGTTCGAACATAAGCCCGGTTGA

mRNA sequence

ATGGTAGATTCTGGTGCCACCCACAATTTCATGACAGAAACCGAAGCACGTCGGCTGAACTTGCGATGGGATAAAGACCTAGGAAAGATGAAAGCGGTCAACTCGGCGGCCCTCTCCATCATGGGAGTTGCAAAAAAAGTTTCAGTAAAGCTAGGGACATGGAGTGGACAGGTCGACTTTATGATAGTGCAGATGGACGACTTCGACAAAGTATTGGAGATGGAATTTCTATTGGAACACAAAGTCATCCCCATGCACCTCGTTAAGTGCCTGGTTGTAACGGGTTCCCATCCCACAATTGTTCAGACAAGCATCAAACAACCAAGCGGAGCGAAGATGATATCAGCTCTCCAGCTAAATAAAGGTCTCGCTCGTGACGAGCCGACCTTCATGGCCATTCTTGTTGTGGAAGGCGGAAATTCTGAAGAGCCAGCTCCTAGAGAAATCCAACGAGTCTTGGAGGTGTATGCCGATACCGAGCCCTTAACAGAATTACTGAAGAAGAACCAGAAGTGGAACTGGACCCCAGAATGTCACGCCGCATTTGAGAGTTTGAAAAAGGTGATGATGGAGGGGCCGGTGTTGGGAATTGCAGACGTCACAAGACCGTTCGAAGTCGAGACTAATGCGTCAGACTTCGCCCTGGGAGGAGTGCTTCTCCAAGACGGTCACCCCATTGCATACGAGAGTCGGAAGTTGAATGATGCTGAAAGGAGGTATGCCGCCTCCAAGAAAGAGATGTTAGCAGTAGTAGTCTACTGCTTGAGGGCCTGGAGGCAATATCTCCTAGGGGCCAAGTTCGTTGTCAAGACCGATAACAGCTCAGTCTGTCACTTCTTCAACCAACCGAAGTTGTCGTCCAAGCAAGCTAGGTGGCAAGAGTACCTTGCCGAGTTCGATTTTCAGTTCGAACATAAGCCCGGTTGA

Coding sequence (CDS)

ATGGTAGATTCTGGTGCCACCCACAATTTCATGACAGAAACCGAAGCACGTCGGCTGAACTTGCGATGGGATAAAGACCTAGGAAAGATGAAAGCGGTCAACTCGGCGGCCCTCTCCATCATGGGAGTTGCAAAAAAAGTTTCAGTAAAGCTAGGGACATGGAGTGGACAGGTCGACTTTATGATAGTGCAGATGGACGACTTCGACAAAGTATTGGAGATGGAATTTCTATTGGAACACAAAGTCATCCCCATGCACCTCGTTAAGTGCCTGGTTGTAACGGGTTCCCATCCCACAATTGTTCAGACAAGCATCAAACAACCAAGCGGAGCGAAGATGATATCAGCTCTCCAGCTAAATAAAGGTCTCGCTCGTGACGAGCCGACCTTCATGGCCATTCTTGTTGTGGAAGGCGGAAATTCTGAAGAGCCAGCTCCTAGAGAAATCCAACGAGTCTTGGAGGTGTATGCCGATACCGAGCCCTTAACAGAATTACTGAAGAAGAACCAGAAGTGGAACTGGACCCCAGAATGTCACGCCGCATTTGAGAGTTTGAAAAAGGTGATGATGGAGGGGCCGGTGTTGGGAATTGCAGACGTCACAAGACCGTTCGAAGTCGAGACTAATGCGTCAGACTTCGCCCTGGGAGGAGTGCTTCTCCAAGACGGTCACCCCATTGCATACGAGAGTCGGAAGTTGAATGATGCTGAAAGGAGGTATGCCGCCTCCAAGAAAGAGATGTTAGCAGTAGTAGTCTACTGCTTGAGGGCCTGGAGGCAATATCTCCTAGGGGCCAAGTTCGTTGTCAAGACCGATAACAGCTCAGTCTGTCACTTCTTCAACCAACCGAAGTTGTCGTCCAAGCAAGCTAGGTGGCAAGAGTACCTTGCCGAGTTCGATTTTCAGTTCGAACATAAGCCCGGTTGA

Protein sequence

MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDFMIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLNKGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYADTEPLTELLKKNQKWNWTPECHAAFESLKKVMMEGPVLGIADVTRPFEVETNASDFALGGVLLQDGHPIAYESRKLNDAERRYAASKKEMLAVVVYCLRAWRQYLLGAKFVVKTDNSSVCHFFNQPKLSSKQARWQEYLAEFDFQFEHKPG
Homology
BLAST of Moc02g15030 vs. NCBI nr
Match: XP_022155185.1 (uncharacterized protein LOC111022320 [Momordica charantia])

HSP 1 Score: 446.4 bits (1147), Expect = 2.0e-121
Identity = 272/544 (50.00%), Postives = 285/544 (52.39%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSGATHNFMTETEARRLNL WDKD GKMKAVNSAAL IMGVAK+VSVKLGTWSG VDF
Sbjct: 441 MVDSGATHNFMTETEARRLNLXWDKDPGKMKAVNSAALPIMGVAKRVSVKLGTWSGLVDF 500

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           +IV+MDDFD VL ++FLLEHKVIPM L KCLVVT S P +VQTSIKQPSG KMISALQL 
Sbjct: 501 VIVRMDDFDVVLGIKFLLEHKVIPMPLAKCLVVTSSDPIVVQTSIKQPSGVKMISALQLK 560

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KG+A+DEPTFMAI V EG NSEE  PREIQRVLE YAD                      
Sbjct: 561 KGIAQDEPTFMAIPVAEGRNSEELVPREIQRVLEAYADVMPDNLPKNLPPRPGIDHEIEL 620

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 621 LPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQKKKDXSLRLCI 680

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 681 DYRALNKLXVRNKXPLPIITDLFDQLHGAKYFSKLDLRSGYYQVFHEYLDKFVVVYLDDI 740

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 741 VVYSPTMEEHQVHLQLVFEKLKQNQLYVKREKCSFAQERISFLGHVIECGRIGMEDGKVK 800

BLAST of Moc02g15030 vs. NCBI nr
Match: KAA0035531.1 (uncharacterized protein E6C27_scaffold285G001970 [Cucumis melo var. makuwa])

HSP 1 Score: 367.1 bits (941), Expect = 1.5e-97
Identity = 224/557 (40.22%), Postives = 262/557 (47.04%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSGATHNF+TE EA+RLNLRW+KD G+MKAVNSAAL I+G+ K+  ++LG WSG VDF
Sbjct: 41  MVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTMIRLGGWSGPVDF 100

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           ++V+MDDFD VL MEFLLEH+VIPM L KCLV+TG  P++VQT ++QP G KMISA+QL 
Sbjct: 101 VVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDLRQPDGLKMISAMQLK 160

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 161 KGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLPKSLPPRRTIDHEIEL 220

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 221 VPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIGPQKLLRNKYPLPIITDLFDRLHGA 280

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 281 KYFSKLDLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHE 340

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 341 YLDKFVVVYLDDIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVI 400

BLAST of Moc02g15030 vs. NCBI nr
Match: TYK08407.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 363.6 bits (932), Expect = 1.7e-96
Identity = 220/545 (40.37%), Postives = 261/545 (47.89%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSG THNF+TE +A+RLNLRW+KD G+MKAVNSAAL I+G+ K+ +++LG WSG VDF
Sbjct: 1   MVDSGVTHNFITEAKAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTTIRLGGWSGLVDF 60

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           ++V++DDFD VL MEFLLEH+VI M L KCLV+TGS P++VQ  ++QP G KMISA+QL 
Sbjct: 61  VVVKIDDFDVVLGMEFLLEHQVILMPLAKCLVITGSTPSVVQVDLRQPDGLKMISAMQLK 120

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 121 KGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLPKSLPPRRMIDHEIEL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRLAKAPYGALVLFQKKKDGNLRSGY 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 YQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDD 300

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 301 IVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKI 360

BLAST of Moc02g15030 vs. NCBI nr
Match: KAA0036604.1 (polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 357.5 bits (916), Expect = 1.2e-94
Identity = 225/586 (38.40%), Postives = 263/586 (44.88%), Query Frame = 0

Query: 1    MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
            MVDSGATHNF+TE EA+RLNLRW+KD G+MKAVNSAAL I+G+ K+  ++LG WSG VDF
Sbjct: 448  MVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTMIRLGGWSGPVDF 507

Query: 61   MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
            ++V+MDDFD VL MEFLLEH+VIPM L KCLV+TG  P++VQT ++QP G KMISA+QL 
Sbjct: 508  VVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDLRQPDGLKMISAMQLK 567

Query: 121  KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
            KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 568  KGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLPKSLPPRRMIDHEIEL 627

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 628  VPGPKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQKKKDGSLRLCI 687

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 688  DYRALNKLTVRNKYPLPIITDLFDCLHGAKYFSKLDLRSGYYQVRIAEGDEPKTTCVTRY 747

Query: 301  ------------------------------------------------------------ 309
                                                                        
Sbjct: 748  GAFEFLVMPFGLTNAPDTFCTLMNQVFHEYLDKFVVVYLDDIVVYSTTMEEHRDHLQKVF 807

BLAST of Moc02g15030 vs. NCBI nr
Match: KAA0059106.1 (reverse transcriptase [Cucumis melo var. makuwa])

HSP 1 Score: 356.3 bits (913), Expect = 2.7e-94
Identity = 225/586 (38.40%), Postives = 264/586 (45.05%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSGATHNF+TE EA+RLNLRW+KD G+MKAVNSAAL I+G+ K+  ++LG WSG VDF
Sbjct: 276 MVDSGATHNFITELEAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTMIRLGGWSGLVDF 335

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           ++V+MDDFD VL MEFLL+H+VIPM LVKCLV+TG  P++VQT ++QP G KMISA+QL 
Sbjct: 336 VVVKMDDFDVVLGMEFLLKHQVIPMPLVKCLVITGPTPSVVQTDLRQPDGLKMISAMQLK 395

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 396 KGLSRDEPTFMAIPLKSSENSGETIPKEIMRVLEKYRDVMPDSLPKSLPPRRMIDHEIEL 455

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 456 VPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQRKKDGSLRLCI 515

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 516 DYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVRIAEGDEPKTTCVTRY 575

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 576 GAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVYSTTMEEHRDHLQKVF 635

BLAST of Moc02g15030 vs. ExPASy Swiss-Prot
Match: P04323 (Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 1.0e-27
Identity = 80/224 (35.71%), Postives = 122/224 (54.46%), Query Frame = 0

Query: 111 AKMISALQLNK-GLARDEPTFMAILVVEGGNSEEP-------------APREIQRVLEV- 170
           AK    LQL+K    + E TF+  ++   G    P              P+EI+  L + 
Sbjct: 390 AKANLKLQLDKCEFLKQETTFLGHVLTPDGIKPNPEKIEAIQKYPIPTKPKEIKAFLGLT 449

Query: 171 ---------YAD-TEPLTELLKKNQKWNWT-PECHAAFESLKKVMMEGPVLGIADVTRPF 230
                    +AD  +P+T+ LKKN K + T PE  +AF+ LK ++ E P+L + D T+ F
Sbjct: 450 GYYRKFIPNFADIAKPMTKCLKKNMKIDTTNPEYDSAFKKLKYLISEDPILKVPDFTKKF 509

Query: 231 EVETNASDFALGGVLLQDGHPIAYESRKLNDAERRYAASKKEMLAVVVYCLRAWRQYLLG 290
            + T+ASD ALG VL QDGHP++Y SR LN+ E  Y+  +KE+LA +V+  + +R YLLG
Sbjct: 510 TLTTDASDVALGAVLSQDGHPLSYISRTLNEHEINYSTIEKELLA-IVWATKTFRHYLLG 569

Query: 291 AKFVVKTDNSSVCHFFNQPKLSSKQARWQEYLAEFDFQFEHKPG 309
             F + +D+  +   +     +SK  RW+  L+EFDF  ++  G
Sbjct: 570 RHFEISSDHQPLSWLYRMKDPNSKLTRWRVKLSEFDFDIKYIKG 612

BLAST of Moc02g15030 vs. ExPASy Swiss-Prot
Match: P20825 (Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaster OX=7227 GN=pol PE=4 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 7.4e-23
Identity = 60/155 (38.71%), Postives = 96/155 (61.94%), Query Frame = 0

Query: 156 YAD-TEPLTELLKKNQKWNWTP-ECHAAFESLKKVMMEGPVLGIADVTRPFEVETNASDF 215
           YAD  +P+T  LKK  K +    E   AFE LK +++  P+L + D  + F + T+AS+ 
Sbjct: 458 YADIAKPMTSCLKKRTKIDTQKLEYIEAFEKLKALIIRDPILQLPDFEKKFVLTTDASNL 517

Query: 216 ALGGVLLQDGHPIAYESRKLNDAERRYAASKKEMLAVVVYCLRAWRQYLLGAKFVVKTDN 275
           ALG VL Q+GHPI++ SR LND E  Y+A +KE+LA +V+  + +R YLLG +F++ +D+
Sbjct: 518 ALGAVLSQNGHPISFISRTLNDHELNYSAIEKELLA-IVWATKTFRHYLLGRQFLIASDH 577

Query: 276 SSVCHFFNQPKLSSKQARWQEYLAEFDFQFEHKPG 309
             +    N  +  +K  RW+  L+E+ F+ ++  G
Sbjct: 578 QPLRWLHNLKEPGAKLERWRVRLSEYQFKIDYIKG 611

BLAST of Moc02g15030 vs. ExPASy Swiss-Prot
Match: Q9UR07 (Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-11 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-21
Identity = 59/159 (37.11%), Postives = 94/159 (59.12%), Query Frame = 0

Query: 159 TEPLTELLKKNQKWNWTPECHAAFESLKKVMMEGPVLGIADVTRPFEVETNASDFALGGV 218
           T PL  LLKK+ +W WTP    A E++K+ ++  PVL   D ++   +ET+ASD A+G V
Sbjct: 663 THPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAV 722

Query: 219 LLQDG-----HPIAYESRKLNDAERRYAASKKEMLAVVVYCLRAWRQYLLGA--KFVVKT 278
           L Q       +P+ Y S K++ A+  Y+ S KEMLA ++  L+ WR YL      F + T
Sbjct: 723 LSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLA-IIKSLKHWRHYLESTIEPFKILT 782

Query: 279 DNSS-VCHFFNQPKLSSKQ-ARWQEYLAEFDFQFEHKPG 309
           D+ + +    N+ +  +K+ ARWQ +L +F+F+  ++PG
Sbjct: 783 DHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPG 820

BLAST of Moc02g15030 vs. ExPASy Swiss-Prot
Match: P0CT41 (Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-12 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-21
Identity = 59/159 (37.11%), Postives = 94/159 (59.12%), Query Frame = 0

Query: 159 TEPLTELLKKNQKWNWTPECHAAFESLKKVMMEGPVLGIADVTRPFEVETNASDFALGGV 218
           T PL  LLKK+ +W WTP    A E++K+ ++  PVL   D ++   +ET+ASD A+G V
Sbjct: 663 THPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAV 722

Query: 219 LLQDG-----HPIAYESRKLNDAERRYAASKKEMLAVVVYCLRAWRQYLLGA--KFVVKT 278
           L Q       +P+ Y S K++ A+  Y+ S KEMLA ++  L+ WR YL      F + T
Sbjct: 723 LSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLA-IIKSLKHWRHYLESTIEPFKILT 782

Query: 279 DNSS-VCHFFNQPKLSSKQ-ARWQEYLAEFDFQFEHKPG 309
           D+ + +    N+ +  +K+ ARWQ +L +F+F+  ++PG
Sbjct: 783 DHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPG 820

BLAST of Moc02g15030 vs. ExPASy Swiss-Prot
Match: P0CT34 (Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=Tf2-1 PE=3 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.8e-21
Identity = 59/159 (37.11%), Postives = 94/159 (59.12%), Query Frame = 0

Query: 159 TEPLTELLKKNQKWNWTPECHAAFESLKKVMMEGPVLGIADVTRPFEVETNASDFALGGV 218
           T PL  LLKK+ +W WTP    A E++K+ ++  PVL   D ++   +ET+ASD A+G V
Sbjct: 663 THPLNNLLKKDVRWKWTPTQTQAIENIKQCLVSPPVLRHFDFSKKILLETDASDVAVGAV 722

Query: 219 LLQDG-----HPIAYESRKLNDAERRYAASKKEMLAVVVYCLRAWRQYLLGA--KFVVKT 278
           L Q       +P+ Y S K++ A+  Y+ S KEMLA ++  L+ WR YL      F + T
Sbjct: 723 LSQKHDDDKYYPVGYYSAKMSKAQLNYSVSDKEMLA-IIKSLKHWRHYLESTIEPFKILT 782

Query: 279 DNSS-VCHFFNQPKLSSKQ-ARWQEYLAEFDFQFEHKPG 309
           D+ + +    N+ +  +K+ ARWQ +L +F+F+  ++PG
Sbjct: 783 DHRNLIGRITNESEPENKRLARWQLFLQDFNFEINYRPG 820

BLAST of Moc02g15030 vs. ExPASy TrEMBL
Match: A0A6J1DLQ6 (uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022320 PE=4 SV=1)

HSP 1 Score: 446.4 bits (1147), Expect = 9.5e-122
Identity = 272/544 (50.00%), Postives = 285/544 (52.39%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSGATHNFMTETEARRLNL WDKD GKMKAVNSAAL IMGVAK+VSVKLGTWSG VDF
Sbjct: 441 MVDSGATHNFMTETEARRLNLXWDKDPGKMKAVNSAALPIMGVAKRVSVKLGTWSGLVDF 500

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           +IV+MDDFD VL ++FLLEHKVIPM L KCLVVT S P +VQTSIKQPSG KMISALQL 
Sbjct: 501 VIVRMDDFDVVLGIKFLLEHKVIPMPLAKCLVVTSSDPIVVQTSIKQPSGVKMISALQLK 560

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KG+A+DEPTFMAI V EG NSEE  PREIQRVLE YAD                      
Sbjct: 561 KGIAQDEPTFMAIPVAEGRNSEELVPREIQRVLEAYADVMPDNLPKNLPPRPGIDHEIEL 620

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 621 LPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQKKKDXSLRLCI 680

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 681 DYRALNKLXVRNKXPLPIITDLFDQLHGAKYFSKLDLRSGYYQVFHEYLDKFVVVYLDDI 740

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 741 VVYSPTMEEHQVHLQLVFEKLKQNQLYVKREKCSFAQERISFLGHVIECGRIGMEDGKVK 800

BLAST of Moc02g15030 vs. ExPASy TrEMBL
Match: A0A5A7T1S6 (Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold285G001970 PE=4 SV=1)

HSP 1 Score: 367.1 bits (941), Expect = 7.3e-98
Identity = 224/557 (40.22%), Postives = 262/557 (47.04%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSGATHNF+TE EA+RLNLRW+KD G+MKAVNSAAL I+G+ K+  ++LG WSG VDF
Sbjct: 41  MVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTMIRLGGWSGPVDF 100

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           ++V+MDDFD VL MEFLLEH+VIPM L KCLV+TG  P++VQT ++QP G KMISA+QL 
Sbjct: 101 VVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDLRQPDGLKMISAMQLK 160

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 161 KGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLPKSLPPRRTIDHEIEL 220

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 221 VPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIGPQKLLRNKYPLPIITDLFDRLHGA 280

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 281 KYFSKLDLRSGYYQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHE 340

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 341 YLDKFVVVYLDDIVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVI 400

BLAST of Moc02g15030 vs. ExPASy TrEMBL
Match: A0A5D3C8W7 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold654G00190 PE=4 SV=1)

HSP 1 Score: 363.6 bits (932), Expect = 8.1e-97
Identity = 220/545 (40.37%), Postives = 261/545 (47.89%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSG THNF+TE +A+RLNLRW+KD G+MKAVNSAAL I+G+ K+ +++LG WSG VDF
Sbjct: 1   MVDSGVTHNFITEAKAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTTIRLGGWSGLVDF 60

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           ++V++DDFD VL MEFLLEH+VI M L KCLV+TGS P++VQ  ++QP G KMISA+QL 
Sbjct: 61  VVVKIDDFDVVLGMEFLLEHQVILMPLAKCLVITGSTPSVVQVDLRQPDGLKMISAMQLK 120

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 121 KGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLPKSLPPRRMIDHEIEL 180

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 181 VPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRLAKAPYGALVLFQKKKDGNLRSGY 240

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 241 YQVRIAEGDEPKTTCVTRYGAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDD 300

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 301 IVVYSTTMEEHRDHLQKVFQKLKENQLYVKREKCSFAQERINFLGHVIECGRIGMEEGKI 360

BLAST of Moc02g15030 vs. ExPASy TrEMBL
Match: A0A5A7SZ91 (Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold191G001280 PE=4 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 5.8e-95
Identity = 225/586 (38.40%), Postives = 263/586 (44.88%), Query Frame = 0

Query: 1    MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
            MVDSGATHNF+TE EA+RLNLRW+KD G+MKAVNSAAL I+G+ K+  ++LG WSG VDF
Sbjct: 448  MVDSGATHNFITEVEAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTMIRLGGWSGPVDF 507

Query: 61   MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
            ++V+MDDFD VL MEFLLEH+VIPM L KCLV+TG  P++VQT ++QP G KMISA+QL 
Sbjct: 508  VVVKMDDFDVVLGMEFLLEHQVIPMPLAKCLVITGPTPSVVQTDLRQPDGLKMISAMQLK 567

Query: 121  KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
            KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 568  KGLSRDEPTFMAIPLKSSENSGETVPKEIMRVLEKYRDVMPDSLPKSLPPRRMIDHEIEL 627

Query: 181  ------------------------------------------------------------ 240
                                                                        
Sbjct: 628  VPGPKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQKKKDGSLRLCI 687

Query: 241  ------------------------------------------------------------ 300
                                                                        
Sbjct: 688  DYRALNKLTVRNKYPLPIITDLFDCLHGAKYFSKLDLRSGYYQVRIAEGDEPKTTCVTRY 747

Query: 301  ------------------------------------------------------------ 309
                                                                        
Sbjct: 748  GAFEFLVMPFGLTNAPDTFCTLMNQVFHEYLDKFVVVYLDDIVVYSTTMEEHRDHLQKVF 807

BLAST of Moc02g15030 vs. ExPASy TrEMBL
Match: A0A5A7UY33 (Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold430G00110 PE=4 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.3e-94
Identity = 225/586 (38.40%), Postives = 264/586 (45.05%), Query Frame = 0

Query: 1   MVDSGATHNFMTETEARRLNLRWDKDLGKMKAVNSAALSIMGVAKKVSVKLGTWSGQVDF 60
           MVDSGATHNF+TE EA+RLNLRW+KD G+MKAVNSAAL I+G+ K+  ++LG WSG VDF
Sbjct: 276 MVDSGATHNFITELEAKRLNLRWEKDAGRMKAVNSAALPIIGLVKRTMIRLGGWSGLVDF 335

Query: 61  MIVQMDDFDKVLEMEFLLEHKVIPMHLVKCLVVTGSHPTIVQTSIKQPSGAKMISALQLN 120
           ++V+MDDFD VL MEFLL+H+VIPM LVKCLV+TG  P++VQT ++QP G KMISA+QL 
Sbjct: 336 VVVKMDDFDVVLGMEFLLKHQVIPMPLVKCLVITGPTPSVVQTDLRQPDGLKMISAMQLK 395

Query: 121 KGLARDEPTFMAILVVEGGNSEEPAPREIQRVLEVYAD---------------------- 180
           KGL+RDEPTFMAI +    NS E  P+EI RVLE Y D                      
Sbjct: 396 KGLSRDEPTFMAIPLKSSENSGETIPKEIMRVLEKYRDVMPDSLPKSLPPRRMIDHEIEL 455

Query: 181 ------------------------------------------------------------ 240
                                                                       
Sbjct: 456 VPGAKPPAKNAYRMAPPELAELRKQLDELLNAGFIRPAKAPYGAPVLFQRKKDGSLRLCI 515

Query: 241 ------------------------------------------------------------ 300
                                                                       
Sbjct: 516 DYRALNKLTVRNKYPLPIITDLFDRLHGAKYFSKLDLRSGYYQVRIAEGDEPKTTCVTRY 575

Query: 301 ------------------------------------------------------------ 309
                                                                       
Sbjct: 576 GAFEFLVMPFGLTNAPATFCTLMNQVFHEYLDKFVVVYLDDIVVYSTTMEEHRDHLQKVF 635

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022155185.12.0e-12150.00uncharacterized protein LOC111022320 [Momordica charantia][more]
KAA0035531.11.5e-9740.22uncharacterized protein E6C27_scaffold285G001970 [Cucumis melo var. makuwa][more]
TYK08407.11.7e-9640.37reverse transcriptase [Cucumis melo var. makuwa][more]
KAA0036604.11.2e-9438.40polyprotein [Cucumis melo var. makuwa][more]
KAA0059106.12.7e-9438.40reverse transcriptase [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P043231.0e-2735.71Retrovirus-related Pol polyprotein from transposon 17.6 OS=Drosophila melanogast... [more]
P208257.4e-2338.71Retrovirus-related Pol polyprotein from transposon 297 OS=Drosophila melanogaste... [more]
Q9UR071.8e-2137.11Transposon Tf2-11 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT411.8e-2137.11Transposon Tf2-12 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 24... [more]
P0CT341.8e-2137.11Transposon Tf2-1 polyprotein OS=Schizosaccharomyces pombe (strain 972 / ATCC 248... [more]
Match NameE-valueIdentityDescription
A0A6J1DLQ69.5e-12250.00uncharacterized protein LOC111022320 OS=Momordica charantia OX=3673 GN=LOC111022... [more]
A0A5A7T1S67.3e-9840.22Reverse transcriptase domain-containing protein OS=Cucumis melo var. makuwa OX=1... [more]
A0A5D3C8W78.1e-9740.37Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold65... [more]
A0A5A7SZ915.8e-9538.40Polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold191G001280 P... [more]
A0A5A7UY331.3e-9438.40Reverse transcriptase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold43... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR041577Reverse transcriptase/retrotransposon-derived protein, RNase H-like domainPFAMPF17919RT_RNaseH_2coord: 174..269
e-value: 5.4E-30
score: 103.3
NoneNo IPR availablePFAMPF08284RVP_2coord: 2..82
e-value: 3.3E-6
score: 26.9
NoneNo IPR availableGENE3D3.10.20.370coord: 204..271
e-value: 5.2E-8
score: 34.8
NoneNo IPR availablePANTHERPTHR24559TRANSPOSON TY3-I GAG-POL POLYPROTEINcoord: 160..290
NoneNo IPR availablePANTHERPTHR24559:SF324TRANSPOSON TY3-I GAG-POL POLYPROTEIN-LIKE PROTEINcoord: 160..290
NoneNo IPR availableCDDcd09274RNase_HI_RT_Ty3coord: 205..308
e-value: 3.19992E-51
score: 163.047
NoneNo IPR availableCDDcd00303retropepsin_likecoord: 1..77
e-value: 3.48446E-12
score: 59.6576
IPR043128Reverse transcriptase/Diguanylate cyclase domainGENE3D3.30.70.270coord: 149..203
e-value: 1.3E-8
score: 36.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 1..104
e-value: 1.9E-13
score: 52.2
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 158..305

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Moc02g15030.1Moc02g15030.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003824 catalytic activity