Moc02g13180 (gene) Bitter gourd (OHB3-1) v2

Overview


Name	Moc02g13180
Type	gene
Organism	Momordica charantia cv. OHB3-1 (Bitter gourd (OHB3-1) v2)
Description	Methionine--tRNA ligase
Location	chr2: 9651871 .. 9655961 (-)
RNA-Seq Expression	Moc02g13180
Synteny	Moc02g13180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAGCTATCTCTTAAGATCAAGGTAGTTTATGGTCGTGGTATCTCTTATGGCCGAGGTTCCCTTATGGCCAAGGTATCCCTTCTAACCGAGGTACCTCTTACGATCGAGCTATCTGATCTTAATGGTTGAACTATCTCTTATGGTCGATAATTGCTCTTCGAAAAATGGATTCGAGACTTCATACTTTAGTTTATGGTCAAGTTTTCTTATTCGTCCACGTGTCACATCGTTTTTTTATCACCTAACCATTATAATGGTAGGATTTGATTGCAACAATTTTATAAATTTGAAGGATAATATTCTTTTAATTGAAAGTTTCAGAGAACTATTACATTGCATCATTCTTGTGTAATTCAGCTATGATTTTTCACATTTTTCCCCATTTTAACTTTATATTTCCCATTTTAACTTTGCAGAGAAAATCCCACATAGGAAATAAAATTTGGTATGAGTTTGAAGTAAGAACACGGACAATAACCACTGTTGACAAAGTAGAAACCTAGCTAATTTTTTTACCATGGAGATTAAACTTACCTACATTCAAATTGACTAAACTTATCTAGAGAGTTAATACTCAAACACGAACATTTATAGTTTTGAGCCACGCATCTACTTGGCGTCATTTTACGTGAATAACATGTGCAACACTCAAGGACTGTAACATGTTGTCTAGACAAACGACTAGGCCAGAATAGTCACTATATATATATAAATAAAATGTAGATTAAAATCTAAATTTTTAAAACCTCTATACTAGATAAATATTATCTTTTAAGGTAAAAAATCTTTTAAAGATAATTAAAAGTTCGATCTCCCACACTCTACCTTTGTTCAACTATATATATATATATATATAAATAAAGAGAGGTCTATGTACTTTCGATTATTGTATCCTTTTGTTGGTGCTTACTAATTAAATTATAGGAATGAAATCAATTAGTATGACCTGACCTGAACTTAAGAATAACCATCTTCCCTAAAAAGTTAAATTTCATTCTAATTTCCTTACAACCTCATATGGATCGATCTTAATTATTCTCATTCCTTATTTTTTGGGCCCACACTTTTCAATAATTATTTTACCATTTTTACCATTTTTTCACTCATCTACTATCGAAATGCATAACTTTTAAATTAATTGTTTTGCAATTTTACTTTCCCTTTAATTTATTTATTTTTACTACCAATAAAAAATTATACACCTATTATTAGTCAATGTGATTTCATTATAAAATAGAATAAATTTCTATCTAATTTATTATCTCAACTAACGGTAGAGGCTAACTATTTATTGTGGTACAAGGATTTAAAATAAGTAAATAATTGACCTAAAATTATTAATTATGTATAGATTAAATAAATAAGTTTAACATGGGAGAATTAGAAACGCAGGGTTTTTCATGACAGCCAATAGAATTTCGGTCGGTGATTGGTTGATTGGGGATACAACTTTGTCAGATATGCCCATTCCTGTGGGACACGTGGACGGCCGAGATCTTATATCCTTGGATTATATATTTTTTTATTTAATTTTTATTTTGTTGTTGGTTGTCCCTTATGCAAGGGGAGATGTACTGAAATTGTTACACTATTAAGACAACACCAATGGACTCTGCCTCCTACTCCCCAACTTACCAATAACATCAATATATGTAACAAAATCATTTTAATATCTTCATAATTTAATCATATGCCCATCTTCGCAATATAATATTCAATTAAAAAAGCTACATTTAGGCCGAAACGTTCAAGGTTTGTTTTGTAATTTCATCCTATATATATATATCAAGGTGTTAATTTTGTGTTATGGATGTCGAGAGACGCGATTCTAGCCTGCTGACATGTCAACAAATGATTCAACGTGAGTTTTAACGTGGGCTTCATTGGCAACCTACAGATACAATTTTATACCAGTGTGTTGGTGGGCCACATGCACTTTAAAACTTAAGTTAGTATTTAGTTTGAATTTATGTAGGTTGAAGCTCTTTCATGCTTAAAAACAATAACAATATTGTAAAAAATTTTAATCGCAAAAACAGGATAACTAGAATTGTAAAAACAAAAAAAACTCCACTAAACGTTACTAAATTAGAATATTTTTTTTAAAGGAGAGTTATATTCGTTTTGGCACTCAAATACCATCTATTTCTAAAAGAATTCAAAGGTTATGGCATATTTAAAAATGAGAGAGTAACAAAAATATGGTATGAATTTCAAAATTTGTTAATACTTCTAATTTTTTTAAAAATGTTACAAATTTGACTTGGTTTATTTTCAAGTGATAAGCATTTTTTTGGCTTGAATTAAAATAAAATGATGTCATTTGTGTAAAATTTTAAACTTGCCGCTATATTTAAAAGTACTTATTTTAATTTTGTCATTATGTAATTGGCTCATTTTCAAATACACTATTTTATTCTACTAATAGACTCAATAAATAAATAAATAAGAACTCTGTTTTTTTCTTTTTCCCAATAAAATTCTACCTAGATATATCCAGCCTAACTCGCTCTTAAGGACAATTTTGAAAATATTTTGGAACCCTCTGTTTTACTTTTACCCTAAATTAGAAAAATCAAAATAAAAAAGAAAGAAAGAAAAAAAAAACCCTAATATGGAGTTGGTTATCGCCTCGCCTAGAGGTGTCCAATTCTCCTATGGAGACCCGTCTCGAATAAGACGGGGACGAAAATTTGTTTTTCTCCACCACCTAAACAGAGATGAGGATGGAAATATGATCGCCATCCCTGACCTACCTTGTTCGAATTTTCTATTAAGATGTTGTAGACCTGGTATGTTTACATTTTATATGAATAATTAGACTAAAATGAAATATTATGTTTTTTTATTAGGTGTTTTAAGAAATTTTTCTACGAGAAATAAACTATGTAAGTGAGTTTTCATCTTGAAGAAAAACCGTTCGGTTCGGTCGATGTAGATCGAAAGAACGGTTCGATTCGATTGGGTTAAAAAACCAATGGAATGAGCGACCTCCATAGAAGTTCCGGTAGAATAAACCCTTCTTCTTGATTGGCCTACGCGCAGAGAAAGAAAGCCATGGAATGATGGAAATGGGCGCTAGAACAAACATTTCACTCTGTTTCCGGCTCTTAAACCCTTTGCAGGATTCGAAATTCGAAACCCACCCATCTCGGAACCGCCTCCATTTCTGTCGAAATCTCTTCTTGTCCCCTTCTTCGTCAAAACGAGCTGTATTCTGTACCTCTGCAACCGATTCCATTTCCAATTCCCATGGCAGCGAAGCCGCAACAGACCCATTTGTTATCACGACCCCACTTTACTATGTCAATGCTTCCCCTCACATGGGGAGCGCTTACACCACCATCGCCCCATTTCAGGTGCCCCTTTTTTCTCAATCGAAAATCTTCATATTTGTAGTTCTGTTACGTTGTTCGTTTGATTTTTTGCTTTTACAGCTCTTCTTATTGGTGTTTTTTTCACTTTGGAAACTGGTGGGGGCGTCTGTATAATGCTGGCTTTGAACAATTTTGGGTTAAGAAACATATTTGGTCTGCCCGTGCAGTACCTGAACAGTATGTAACATTTTAGTTTAAAATTTATGACGATTTAGTCAACTAATTTCTATAGTTTTAAATTTGTAACGCAGTCCTTATAATGAAAATTTTGGACAAGGTGAAGTATTAATCTATAGCATGTACAATCATATAGTTTAAATGCACATTTGGCCCCTAGATGGTTTATACGAAGTATCAATTTTGAGAAACTTCTCTATTACAACGTTGACAAAAATCTTCATATGCAAGTATTAGTTGTTACATACTAAAGTTAATGGACTAAATTGTTATAAATTTAAACTATATGGACTAAGTTCAACAAAATTTAGGGACTAAATTATTAGTTTGATGAAGTACCAAACCATATGTGTTTTCCAGTAGCTATTTTTCCTGGGGTATGGATGCATTTTACAGTCCTTAGTTCTTCAATTTTCGATATTAATTCTCTTGATTATTATAAGAACATTTGTCAACCTGCAGGATGAGAAAGAAATTCTTGCCAACAATTGTTGTCCTATACACCTAAATCCCTGTGCTTTAAGGAAATAA

mRNA sequence

ATGGCCGAGCTATCTCTTAAGATCAAGGTAGTTTATGGTCGTGGTATCTCTTATGGCCGAGGTTCCCTTATGGCCAAGGTATCCCTTCTAACCGAGGATTCGAAATTCGAAACCCACCCATCTCGGAACCGCCTCCATTTCTGTCGAAATCTCTTCTTGTCCCCTTCTTCGTCAAAACGAGCTGTATTCTGTACCTCTGCAACCGATTCCATTTCCAATTCCCATGGCAGCGAAGCCGCAACAGACCCATTTGTTATCACGACCCCACTTTACTATGTCAATGCTTCCCCTCACATGGGGAGCGCTTACACCACCATCGCCCCATTTCAGGATGAGAAAGAAATTCTTGCCAACAATTGTTGTCCTATACACCTAAATCCCTGTGCTTTAAGGAAATAA

Coding sequence (CDS)

ATGGCCGAGCTATCTCTTAAGATCAAGGTAGTTTATGGTCGTGGTATCTCTTATGGCCGAGGTTCCCTTATGGCCAAGGTATCCCTTCTAACCGAGGATTCGAAATTCGAAACCCACCCATCTCGGAACCGCCTCCATTTCTGTCGAAATCTCTTCTTGTCCCCTTCTTCGTCAAAACGAGCTGTATTCTGTACCTCTGCAACCGATTCCATTTCCAATTCCCATGGCAGCGAAGCCGCAACAGACCCATTTGTTATCACGACCCCACTTTACTATGTCAATGCTTCCCCTCACATGGGGAGCGCTTACACCACCATCGCCCCATTTCAGGATGAGAAAGAAATTCTTGCCAACAATTGTTGTCCTATACACCTAAATCCCTGTGCTTTAAGGAAATAA

Protein sequence

MAELSLKIKVVYGRGISYGRGSLMAKVSLLTEDSKFETHPSRNRLHFCRNLFLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHMGSAYTTIAPFQDEKEILANNCCPIHLNPCALRK

Homology

[Close]

BLAST of Moc02g13180 vs. NCBI nr
Match: KAG6734771.1 (hypothetical protein I3842_01G286200 [Carya illinoinensis])

HSP 1 Score: 106.3 bits (264), Expect = 2.1e-19
Identity = 59/101 (58.42%), Postives = 65/101 (64.36%), Query Frame = 0


Query: 44  RLHFCRNLFL-------SPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNAS 103
           RLHF R  F        S  SSKRA+FCT  + S  N+   +    PFVITTPLYYVNA 
Sbjct: 31  RLHFRRQPFFVSSTSSSSSMSSKRALFCTCTSSSADNNGLHDGG--PFVITTPLYYVNAP 90

Query: 104 PHMGSAYTTIAP-----FQDEKEILANNCCPIHLNPCALRK 133
           PHMGSAYTTIA      FQDEKE+L NNCCP+HL PC  RK
Sbjct: 91  PHMGSAYTTIAADAIARFQDEKELLDNNCCPMHLKPCVSRK 129

[Close]

BLAST of Moc02g13180 vs. NCBI nr
Match: XP_004142742.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284.1 hypothetical protein Csa_008274 [Cucumis sativus])

HSP 1 Score: 97.4 bits (241), Expect = 9.5e-17
Identity = 52/68 (76.47%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           R RLHFC+NL  F S SSSKRA+FCTS ++   NSH S AATDPFVITTPLYYVNA+PHM
Sbjct: 31  RTRLHFCQNLLSFPSHSSSKRAIFCTSTSN--FNSHDSGAATDPFVITTPLYYVNAAPHM 90

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 91  GSAYTTIA 96

[Close]

BLAST of Moc02g13180 vs. NCBI nr
Match: XP_022133974.1 (methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia])

HSP 1 Score: 93.2 bits (230), Expect = 1.8e-15
Identity = 70/198 (35.35%), Postives = 78/198 (39.39%), Query Frame = 0


Query: 42  RNRLHFCRNLFLSP----SSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASP 101
           R RL+F RNL  SP    SSSKRA+FCT A++   NSH S AATDPFVITTPLYYVNA+P
Sbjct: 34  RTRLNFYRNLLPSPSYSASSSKRAIFCTYASN--CNSHESGAATDPFVITTPLYYVNAAP 93

Query: 102 HMGSAYTTIA-------------------------------------------------- 133
           HMGSAYTTIA                                                  
Sbjct: 94  HMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAY 153

[Close]

BLAST of Moc02g13180 vs. NCBI nr
Match: KAA0066020.1 (methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ligase [Cucumis melo var. makuwa])

HSP 1 Score: 92.0 bits (227), Expect = 4.0e-15
Identity = 51/68 (75.00%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           R RLHF +NL  F S SSSKRA+FCT  T S  NSH + AATDPFVITTPLYYVNA+PHM
Sbjct: 31  RTRLHFYQNLLSFASYSSSKRAIFCT--TTSNFNSHDTGAATDPFVITTPLYYVNAAPHM 90

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 91  GSAYTTIA 96

[Close]

BLAST of Moc02g13180 vs. NCBI nr
Match: XP_008463474.1 (PREDICTED: LOW QUALITY PROTEIN: methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis melo])

HSP 1 Score: 92.0 bits (227), Expect = 4.0e-15
Identity = 51/68 (75.00%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           R RLHF +NL  F S SSSKRA+FCT  T S  NSH + AATDPFVITTPLYYVNA+PHM
Sbjct: 31  RTRLHFYQNLLSFASYSSSKRAIFCT--TTSNFNSHDTGAATDPFVITTPLYYVNAAPHM 90

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 91  GSAYTTIA 96

[Close]

BLAST of Moc02g13180 vs. ExPASy Swiss-Prot
Match: Q9M2T9 (Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=OVA1 PE=2 SV=1)

HSP 1 Score: 62.8 bits (151), Expect = 3.4e-09
Identity = 35/68 (51.47%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           RN   F + L  +   SS KRA++CTS++   +   G     + FV+TTPLYYVNA PHM
Sbjct: 32  RNSFRFSKKLPYYSQFSSGKRALYCTSSSQESTVDEG-----ETFVLTTPLYYVNAPPHM 91

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 92  GSAYTTIA 94

HSP 2 Score: 43.9 bits (102), Expect = 1.6e-03
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 0


Query: 109 FQDEKEILANNCCPIHLNPCALRK 133
           ++DEKE+L NNCCP+H  PC  RK
Sbjct: 199 YKDEKELLENNCCPVHQMPCVARK 222

[Close]

BLAST of Moc02g13180 vs. ExPASy Swiss-Prot
Match: Q9C2H9 (Probable methionine--tRNA ligase, mitochondrial OS=Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) OX=367110 GN=93G11.130 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 2.5e-04
Identity = 22/59 (37.29%), Postives = 39/59 (66.10%), Query Frame = 0


Query: 48  CRNLFLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHMGSAYTTI 107
           CR+ F++P +++R +  TS T +   +  ++    PF +TTP++YVNASPH+G  Y+ +
Sbjct: 31  CRSQFVAPKAARRTL-ATSTTTTTPTAPDNK----PFYVTTPIFYVNASPHVGHMYSMV 84

[Close]

BLAST of Moc02g13180 vs. ExPASy Swiss-Prot
Match: P59081 (Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221 GN=metG PE=3 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.3e-04
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 84  FVITTPLYYVNASPHMGSAYTTIA 108
           F +TTPLYYVNA PH+GSAYTTIA
Sbjct: 5   FSLTTPLYYVNALPHIGSAYTTIA 28

[Close]

BLAST of Moc02g13180 vs. ExPASy Swiss-Prot
Match: O33925 (Methionine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=metG PE=3 SV=1)

HSP 1 Score: 44.7 bits (104), Expect = 9.6e-04
Identity = 18/23 (78.26%), Postives = 21/23 (91.30%), Query Frame = 0


Query: 84  FVITTPLYYVNASPHMGSAYTTI 107
           F ITTP+YYVN+ PH+GSAYTTI
Sbjct: 3   FYITTPIYYVNSEPHIGSAYTTI 25

[Close]

BLAST of Moc02g13180 vs. ExPASy TrEMBL
Match: A0A0A0KQV7 (Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 4.6e-17
Identity = 52/68 (76.47%), Postives = 57/68 (83.82%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           R RLHFC+NL  F S SSSKRA+FCTS ++   NSH S AATDPFVITTPLYYVNA+PHM
Sbjct: 31  RTRLHFCQNLLSFPSHSSSKRAIFCTSTSN--FNSHDSGAATDPFVITTPLYYVNAAPHM 90

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 91  GSAYTTIA 96

[Close]

BLAST of Moc02g13180 vs. ExPASy TrEMBL
Match: A0A6J1BXG8 (Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.7e-16
Identity = 70/198 (35.35%), Postives = 78/198 (39.39%), Query Frame = 0


Query: 42  RNRLHFCRNLFLSP----SSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASP 101
           R RL+F RNL  SP    SSSKRA+FCT A++   NSH S AATDPFVITTPLYYVNA+P
Sbjct: 34  RTRLNFYRNLLPSPSYSASSSKRAIFCTYASN--CNSHESGAATDPFVITTPLYYVNAAP 93

Query: 102 HMGSAYTTIA-------------------------------------------------- 133
           HMGSAYTTIA                                                  
Sbjct: 94  HMGSAYTTIAADAIARFQRLLGKKVIFITGTDEHGEKIATAAAAQGSAPSEHCDAISEAY 153

[Close]

BLAST of Moc02g13180 vs. ExPASy TrEMBL
Match: A0A5D3C1K0 (Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold98G003330 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-15
Identity = 51/68 (75.00%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           R RLHF +NL  F S SSSKRA+FCT  T S  NSH + AATDPFVITTPLYYVNA+PHM
Sbjct: 31  RTRLHFYQNLLSFASYSSSKRAIFCT--TTSNFNSHDTGAATDPFVITTPLYYVNAAPHM 90

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 91  GSAYTTIA 96

[Close]

BLAST of Moc02g13180 vs. ExPASy TrEMBL
Match: A0A1S3CJS7 (Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 1.9e-15
Identity = 51/68 (75.00%), Postives = 55/68 (80.88%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           R RLHF +NL  F S SSSKRA+FCT  T S  NSH + AATDPFVITTPLYYVNA+PHM
Sbjct: 31  RTRLHFYQNLLSFASYSSSKRAIFCT--TTSNFNSHDTGAATDPFVITTPLYYVNAAPHM 90

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 91  GSAYTTIA 96

[Close]

BLAST of Moc02g13180 vs. ExPASy TrEMBL
Match: A0A803P2R1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 2.1e-14
Identity = 56/143 (39.16%), Postives = 66/143 (46.15%), Query Frame = 0


Query: 46  HFCRNLFLSPSS-SKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHMGSAYT 105
           HF RN+   PSS S RAVFC+SA  + +    +E  +DPFV+TTPLYYVNA PHMGSAYT
Sbjct: 38  HFRRNI---PSSFSHRAVFCSSAASNSTPLEPNEVVSDPFVLTTPLYYVNAPPHMGSAYT 97

Query: 106 TIA-------------------------------------------------------PF 133
           TIA                                                        +
Sbjct: 98  TIAADAIARFQLDIAYDKFIRTTDPKHEYIVKEFYSRVLANGDIYRADYEGLYCINCEEY 157

[Close]

BLAST of Moc02g13180 vs. TAIR 10
Match: AT3G55400.1 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 62.8 bits (151), Expect = 2.4e-10
Identity = 35/68 (51.47%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           RN   F + L  +   SS KRA++CTS++   +   G     + FV+TTPLYYVNA PHM
Sbjct: 32  RNSFRFSKKLPYYSQFSSGKRALYCTSSSQESTVDEG-----ETFVLTTPLYYVNAPPHM 91

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 92  GSAYTTIA 94

HSP 2 Score: 43.9 bits (102), Expect = 1.2e-04
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 0


Query: 109 FQDEKEILANNCCPIHLNPCALRK 133
           ++DEKE+L NNCCP+H  PC  RK
Sbjct: 199 YKDEKELLENNCCPVHQMPCVARK 222

[Close]

BLAST of Moc02g13180 vs. TAIR 10
Match: AT3G55400.2 (methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS) )

HSP 1 Score: 62.8 bits (151), Expect = 2.4e-10
Identity = 35/68 (51.47%), Postives = 44/68 (64.71%), Query Frame = 0


Query: 42  RNRLHFCRNL--FLSPSSSKRAVFCTSATDSISNSHGSEAATDPFVITTPLYYVNASPHM 101
           RN   F + L  +   SS KRA++CTS++   +   G     + FV+TTPLYYVNA PHM
Sbjct: 32  RNSFRFSKKLPYYSQFSSGKRALYCTSSSQESTVDEG-----ETFVLTTPLYYVNAPPHM 91

Query: 102 GSAYTTIA 108
           GSAYTTIA
Sbjct: 92  GSAYTTIA 94

HSP 2 Score: 43.9 bits (102), Expect = 1.2e-04
Identity = 15/24 (62.50%), Postives = 19/24 (79.17%), Query Frame = 0


Query: 109 FQDEKEILANNCCPIHLNPCALRK 133
           ++DEKE+L NNCCP+H  PC  RK
Sbjct: 199 YKDEKELLENNCCPVHQMPCVARK 222

The following BLAST results are available for this feature:

BLAST of Moc02g13180 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6734771.1	2.1e-19	58.42	hypothetical protein I3842_01G286200 [Carya illinoinensis]	[more]
XP_004142742.1	9.5e-17	76.47	methionine--tRNA ligase, chloroplastic/mitochondrial [Cucumis sativus] >KGN51284...	[more]
XP_022133974.1	1.8e-15	35.35	methionine--tRNA ligase, chloroplastic/mitochondrial [Momordica charantia]	[more]
KAA0066020.1	4.0e-15	75.00	methionine--tRNA ligase [Cucumis melo var. makuwa] >TYK05823.1 methionine--tRNA ...	[more]
XP_008463474.1	4.0e-15	75.00	PREDICTED: LOW QUALITY PROTEIN: methionine--tRNA ligase, chloroplastic/mitochond...	[more]

BLAST of Moc02g13180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2T9	3.4e-09	51.47	Methionine--tRNA ligase, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=...	[more]
Q9C2H9	2.5e-04	37.29	Probable methionine--tRNA ligase, mitochondrial OS=Neurospora crassa (strain ATC...	[more]
P59081	3.3e-04	83.33	Methionine--tRNA ligase OS=Thermosynechococcus elongatus (strain BP-1) OX=197221...	[more]
O33925	9.6e-04	78.26	Methionine--tRNA ligase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / J...	[more]

BLAST of Moc02g13180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KQV7	4.6e-17	76.47	Methionine--tRNA ligase OS=Cucumis sativus OX=3659 GN=Csa_5G512910 PE=3 SV=1	[more]
A0A6J1BXG8	8.7e-16	35.35	Methionine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111006379 PE=3 SV=1	[more]
A0A5D3C1K0	1.9e-15	75.00	Methionine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3CJS7	1.9e-15	75.00	Methionine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103501636 PE=3 SV=1	[more]
A0A803P2R1	2.1e-14	39.16	Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1	[more]

BLAST of Moc02g13180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G55400.1	2.4e-10	51.47	methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS)	[more]
AT3G55400.2	2.4e-10	51.47	methionyl-tRNA synthetase / methionine--tRNA ligase / MetRS (cpMetRS)	[more]

InterPro

Analysis Name: InterPro Annotations of Bitter gourd (OHB3-1) v2
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014729	Rossmann-like alpha/beta/alpha sandwich fold	GENE3D	3.40.50.620	HUPs	coord: 74..110 e-value: 5.3E-7 score: 31.3
None	No IPR available	PANTHER	PTHR43326	METHIONYL-TRNA SYNTHETASE	coord: 57..107
None	No IPR available	PANTHER	PTHR43326	METHIONYL-TRNA SYNTHETASE	coord: 109..132
None	No IPR available	PANTHER	PTHR43326:SF6	BNAA09G34980D PROTEIN	coord: 57..107
None	No IPR available	PANTHER	PTHR43326:SF6	BNAA09G34980D PROTEIN	coord: 109..132

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Moc02g13180.1	Moc02g13180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006431	methionyl-tRNA aminoacylation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004825	methionine-tRNA ligase activity